Microorganism sorting device on micro-fluidic chip

Abstract

The utility model discloses a microorganism sorting device on a micro-fluidic chip, and relates to the technical field of microorganisms. The sorting device for the microorganisms on the micro-fluidic chip comprises a base, and a sorting mechanism and a liquid conveying mechanism are arranged above the base; the sorting mechanism and the liquid conveying mechanism are arranged in parallel; the device has the technical effects that a sorting box, a curing lamp, a light-gathering cover, a light-transmitting plate, a cleaning frame, a material receiving frame and other parts in the sorting mechanism are matched with one another, precise and efficient microorganism sorting and convenient cleaning and maintenance are achieved, after microorganism suspension enters the sorting box and stands, microorganisms fall into micro holes, and the microorganisms are separated from the micro holes. Subsequently conveyed curing liquid is cured under the action of a light gathering cover and a light transmitting plate through a curing lamp, microorganism suspension in micro holes is precisely fixed, the sorting precision and efficiency are greatly improved, a cleaning frame is in sliding connection with the light gathering cover, a stirring frame is matched, and the cleaning frame can be driven to clean the light gathering cover by manually moving the stirring frame.

Description

Technical Field

[0001] This utility model relates to the field of microbial technology, specifically to a microbial sorting device on a microfluidic chip. Background Technology

[0002] In the field of microbial research and application, microbial sorting is a crucial operation, and its efficiency and accuracy directly affect the quality of subsequent research and applications. Microfluidic chip technology, as an emerging technique, has shown great potential in microbial sorting due to its advantages such as ease of operation, small sample volume, and fast analysis speed.

[0003] Existing microbial sorting devices on microfluidic chips require a focusing mask to concentrate the light from the curing lamp during the curing process. However, impurities and dust easily accumulate inside the focusing mask, affecting the focusing effect and sorting accuracy. Therefore, this paper proposes a microbial sorting device on a microfluidic chip. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a microbial sorting device on a microfluidic chip, which solves the problem that impurities and dust easily accumulate inside the light-gathering cover, affecting the light-gathering effect and the accuracy of sorting.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, this utility model is implemented through the following technical solution: it includes a base, and a sorting mechanism and an infusion mechanism are arranged on the top of the base;

[0008] The sorting mechanism is arranged in parallel with the infusion mechanism;

[0009] The sorting mechanism includes a sorting section and a cleaning section;

[0010] The sorting section includes a focusing cover and a sorting box, and the cleaning section includes a cleaning rack and a receiving frame.

[0011] The bottom surface of the sorting box is fixedly connected to the top surface of the base. A fixing frame is fixedly installed on the outer wall of the sorting box. A curing lamp is fixedly installed on the inner side of the fixing frame. The inner side of the fixing frame is fixedly connected to the top surface of the focusing cover. The inner side of the focusing cover is slidably connected to the outer wall of the cleaning frame. A light-transmitting plate is fixedly installed on the inner side of the sorting box.

[0012] Preferably, the infusion mechanism includes a micro pump, the bottom surface of which is fixedly connected to the top surface of the base, and a connecting pipe is fixedly provided through the outlet end of the micro pump. The connecting pipe extends through the outer wall of the sorting box to the inner side of the sorting box on one side near the sorting box, and a one-way valve is provided on the inner side of the connecting pipe.

[0013] Preferably, a positioning rail is fixedly provided on the bottom surface of the cleaning frame, and a connecting sleeve is slidably sleeved on the outer wall of the positioning rail, with the bottom surface of the connecting sleeve fixedly connected to the top surface of the receiving frame.

[0014] Preferably, a screw is fixedly provided on the outer wall of the positioning rail, the outer wall of the screw extends through the inner side of the connecting sleeve to the outer side of the connecting sleeve, and a nut is threaded on the outer wall of the screw, the side of the nut near the connecting sleeve is in contact with the outer wall of the connecting sleeve.

[0015] Preferably, a positioning rod is slidably provided on the inner side of the cleaning frame, and the outer wall of the positioning rod is fixedly connected to the inner side of the focusing cover. A toggle frame is fixedly provided on the bottom surface of the cleaning frame, and a liquid outlet pipe is fixedly provided through the outer wall of the sorting box, extending to the inner side of the sorting box. A valve is provided on the inner side of the liquid outlet pipe.

[0016] Preferably, a liquid storage tank is fixedly installed on the top surface of the base, and an inlet pipe is fixedly installed through the top surface of the liquid storage tank, extending into the interior of the liquid storage tank. The inlet pipe of the micro pump is fixedly installed through the outer wall of the liquid storage tank, extending into the inner side of the liquid storage tank.

[0017] (III) Beneficial Effects

[0018] This invention provides a microbial sorting device on a microfluidic chip. It has the following advantages:

[0019] (I) This microbial sorting device on a microfluidic chip, in which the sorting mechanism includes a sorting box, curing lamp, focusing cover, light-transmitting plate, cleaning rack, and receiving frame, works together to achieve precise and efficient microbial sorting and convenient cleaning and maintenance. When the microbial suspension enters the sorting box and settles, the microorganisms fall into the micro-holes. The subsequently transported curing liquid is cured by the curing lamp through the focusing cover and light-transmitting plate, accurately fixing the microbial suspension in the micro-holes, greatly improving the sorting accuracy and efficiency. The cleaning rack is slidably connected to the focusing cover. With the help of the toggle frame, the cleaning rack can be manually moved to clean the focusing cover, avoiding dust and impurities from affecting the focusing effect and ensuring that the curing lamp effectively cures the liquid. The impurities generated during cleaning fall into the receiving frame. The receiving frame is easy to install and disassemble through a combination structure of positioning rail, connecting sleeve, screw and nut, which facilitates the cleaning of impurities.

[0020] (II) This microfluidic chip-based microbial sorting device achieves precise liquid delivery control and reliable performance through a rationally designed structure and component configuration. The micropump precisely regulates the delivery volume and speed of the microbial suspension, solidified liquid, and solidified lysis solution, ensuring uniform liquid distribution within the sorting chamber and smooth solidification and lysis processes, thereby improving the success rate and accuracy of microbial sorting. A one-way valve inside the connecting tube effectively prevents liquid backflow, avoids interference between different liquids, and ensures the accuracy and reliability of the sorting process. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0022] Figure 2 This is a schematic diagram of the sorting mechanism of this utility model;

[0023] Figure 3 This is a schematic diagram of the internal structure of the sorting mechanism of this utility model;

[0024] Figure 4 This is a schematic diagram of the infusion mechanism of this utility model;

[0025] Figure 5 This utility model Figure 3 A magnified structural diagram of region A in the middle.

[0026] In the diagram: 1. Base; 2. Sorting mechanism; 21. Fixing frame; 22. Focusing cover; 23. Sorting box; 24. Curing lamp; 25. Cleaning rack; 26. Actuating rack; 27. Liquid outlet pipe; 28. Light-transmitting plate; 29. ​​Positioning rod; 210. Material receiving frame; 211. Screw; 212. Connecting sleeve; 213. Nut; 214. Positioning rail; 3. Liquid delivery mechanism; 31. Liquid storage tank; 32. Liquid inlet pipe; 33. Micro pump; 34. Connecting pipe. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] Please see Figure 1-5 The present invention provides a technical solution: including a base 1, and a sorting mechanism 2 and an infusion mechanism 3 are arranged on the top of the base 1;

[0029] The sorting mechanism 2 and the infusion mechanism 3 are arranged in parallel.

[0030] Sorting facility 2 includes a sorting department and a cleaning department;

[0031] The sorting section includes a focusing cover 22 and a sorting box 23, and the cleaning section includes a cleaning frame 25 and a receiving frame 210.

[0032] The bottom surface of the sorting box 23 is fixedly connected to the top surface of the base 1. A fixing frame 21 is fixedly installed on the outer wall of the sorting box 23. A curing lamp 24 is fixedly installed on the inner side of the fixing frame 21. The inner side of the fixing frame 21 is fixedly connected to the top surface of the focusing cover 22. The inner side of the focusing cover 22 is slidably connected to the outer wall of the cleaning frame 25. A light-transmitting plate 28 is fixedly installed on the inner side of the sorting box 23. A positioning rail 214 is fixedly installed on the bottom surface of the cleaning frame 25. A connecting sleeve 212 is slidably fitted on the outer wall of the positioning rail 214. The bottom surface of the connecting sleeve 212 is fixedly connected to the top surface of the receiving frame 210. The outer wall of the positioning rail 214 is fixedly equipped with a light-transmitting plate 28. A screw 211 is provided, the outer wall of the screw 211 extends through the inner side of the connecting sleeve 212 to the outer side of the connecting sleeve 212, a nut 213 is threaded on the outer wall of the screw 211, the side of the nut 213 near the connecting sleeve 212 contacts the outer wall of the connecting sleeve 212, a positioning rod 29 is slidably provided on the inner side of the cleaning frame 25, the outer wall of the positioning rod 29 is fixedly connected to the inner side of the focusing cover 22, a toggle frame 26 is fixedly provided on the bottom surface of the cleaning frame 25, and a liquid outlet pipe 27 is fixedly provided through the outer wall of the sorting box 23 and extends to the inner side of the sorting box 23, and a valve is provided on the inner side of the liquid outlet pipe 27;

[0033] The infusion mechanism 3 includes a micro pump 33, the bottom surface of which is fixedly connected to the top surface of the base 1. A connecting pipe 34 is fixedly provided through the outlet end of the micro pump 33. The side of the connecting pipe 34 near the sorting box 23 is fixedly provided through the outer wall of the sorting box 23 and extends to the inside of the sorting box 23. A one-way valve is provided inside the connecting pipe 34. A liquid storage tank 31 is fixedly provided on the top surface of the base 1. An inlet pipe 32 is fixedly provided through the top surface of the liquid storage tank 31 and extends to the inside of the liquid storage tank 31. The inlet pipe of the micro pump 33 is fixedly provided through the outer wall of the liquid storage tank 31 and extends to the inside of the liquid storage tank 31.

[0034] In use, firstly, a microbial suspension is delivered into the storage tank 31. Then, the micropump 33 is turned on, drawing the microbial suspension into the sorting box 23. After the microbial suspension enters the sorting box 23, it is allowed to stand. The microorganisms in the suspension can fall into the micro-holes inside the sorting box 23. After standing for a period of time, the solidified liquid is delivered into the storage tank 31. The micropump 33 is then turned on again, delivering the solidified liquid into the sorting box 23. Due to its higher concentration, the solidified liquid can enter the micro-holes inside the sorting box 23. When the curing lamp 24 is turned on, the curing liquid can be cured through the light-concentrating cover 22 and the light-transmitting plate 28, thereby curing the microbial suspension inside the micropores. After curing, the valve is opened and the uncured liquid is discharged through the liquid outlet pipe 27. After the discharge is completed, the valve is closed and the curing lysis liquid is transported into the liquid storage tank 31. The curing lysis liquid can be transported into the sorting box 23 through the micro pump 33, thereby melting the solidified microorganisms. After melting, it can be discharged through the liquid outlet pipe 27, thereby achieving the purpose of sorting microorganisms.

[0035] During use, the focusing cover 22 can be cleaned to prevent dust from affecting the focusing effect. Cleaning is done by manually controlling the movement of the actuating frame 26. When the actuating frame 26 moves, it can drive the cleaning frame 25 to move. During the movement of the cleaning frame 25, the focusing cover 22 can be cleaned. The impurities generated during cleaning will fall into the inside of the receiving frame 210. After cleaning, the receiving frame 210 can be removed by manually unscrewing the nut 213 from the outer wall of the screw 211. After unscrewing, the receiving frame 210 can be removed. During installation, it can be installed through the positioning rail 214 and the connecting sleeve 212. After installation, the nut 213 is connected to the screw 211 to fix the receiving frame 210.

[0036] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0037] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A microbial sorting device on a microfluidic chip, comprising a base (1), characterized in that: A sorting mechanism (2) and an infusion mechanism (3) are provided above the base (1); The sorting mechanism (2) is arranged in parallel with the infusion mechanism (3); The sorting mechanism (2) includes a sorting section and a cleaning section; The sorting section includes a focusing cover (22) and a sorting box (23), and the cleaning section includes a cleaning rack (25) and a receiving box (210); The bottom surface of the sorting box (23) is fixedly connected to the top surface of the base (1). A fixing frame (21) is fixedly installed on the outer wall of the sorting box (23). A curing lamp (24) is fixedly installed on the inner side of the fixing frame (21). The inner side of the fixing frame (21) is fixedly connected to the top surface of the light-concentrating cover (22). The inner side of the light-concentrating cover (22) is slidably connected to the outer wall of the cleaning frame (25). A light-transmitting plate (28) is fixedly installed on the inner side of the sorting box (23).

2. The microbial sorting device on a microfluidic chip according to claim 1, characterized in that: The infusion mechanism (3) includes a micro pump (33), the bottom surface of which is fixedly connected to the top surface of the base (1), and a connecting pipe (34) is fixedly provided through the outlet end of the micro pump (33). The connecting pipe (34) extends through the outer wall of the sorting box (23) to the inner side of the sorting box (23) on one side near the sorting box (23), and a one-way valve is provided on the inner side of the connecting pipe (34).

3. The microbial sorting device on a microfluidic chip according to claim 1, characterized in that: The bottom surface of the cleaning frame (25) is fixedly provided with a positioning rail (214), and a connecting sleeve (212) is slidably sleeved on the outer wall of the positioning rail (214). The bottom surface of the connecting sleeve (212) is fixedly connected to the top surface of the receiving frame (210).

4. The microbial sorting device on a microfluidic chip according to claim 3, characterized in that: A screw (211) is fixedly installed on the outer wall of the positioning rail (214). The outer wall of the screw (211) extends through the inner side of the connecting sleeve (212) to the outer side of the connecting sleeve (212). A nut (213) is threaded on the outer wall of the screw (211). The side of the nut (213) near the connecting sleeve (212) contacts the outer wall of the connecting sleeve (212).

5. The microbial sorting device on a microfluidic chip according to claim 1, characterized in that: A positioning rod (29) is slidably provided on the inner side of the cleaning rack (25). The outer wall of the positioning rod (29) is fixedly connected to the inner side of the focusing cover (22). A toggle frame (26) is fixedly provided on the bottom surface of the cleaning rack (25). A liquid outlet pipe (27) is fixedly provided through the outer wall of the sorting box (23) and extends to the inner side of the sorting box (23). A valve is provided on the inner side of the liquid outlet pipe (27).

6. The microbial sorting device on a microfluidic chip according to claim 2, characterized in that: A liquid storage tank (31) is fixedly installed on the top surface of the base (1). An inlet pipe (32) is fixedly installed through the top surface of the liquid storage tank (31) and extends into the interior of the liquid storage tank (31). The inlet pipe of the micro pump (33) is fixedly installed through the outer wall of the liquid storage tank (31) and extends into the inner side of the liquid storage tank (31).

Images