Automatic sampling device of fermentation tank

Abstract

The utility model relates to the technical field of biological fermentation equipment, in particular to an automatic sampling device of a fermentation tank, which comprises a sampling pipe, an electric diaphragm valve, a peristaltic pump, a connecting pipe, a controller, an air inlet component and a plurality of groups of sampling components, the electric diaphragm valve is arranged on the sampling pipe, the air inlet assembly and the sampling assemblies are connected with the connecting pipe, the controller is connected with the electric diaphragm valve, the peristaltic pump, the air inlet assembly and the sampling assemblies, the air inlet assembly comprises an air inlet pipe and an air pinch valve, the air inlet pipe is communicated with the connecting pipe, and the air pinch valve is arranged on the air inlet pipe. In this way, the technical problems that manual sampling is adopted in the sampling mode in the prior art, the fermentation process needs to be frequently interrupted, and the contamination risk is increased are solved.

Description

Technical Field

[0001] This utility model relates to the field of biological fermentation equipment technology, and in particular to an automatic sampling device for fermentation tanks. Background Technology

[0002] With the booming development of the bio-fermentation industry, fermentation technology is widely used in many fields such as food, medicine, bioenergy, and agricultural biopharmaceuticals. As the core equipment in the bio-fermentation process, the fermenter plays a crucial role in providing a suitable environment for microbial growth and metabolism, enabling microorganisms to transform raw materials into target fermentation products. The fermentation process is a complex and dynamic system; the growth and reproduction of microorganisms, the generation and accumulation of metabolites, and other processes all evolve continuously with the passage of fermentation time and changes in environmental conditions.

[0003] To accurately monitor the fermentation process and ensure it proceeds as expected, ultimately yielding high-quality, high-yield fermentation products, real-time and accurate sampling and testing of the fermentation broth in the fermenter is essential. Regular sampling and analysis allow for timely understanding of key parameters such as changes in the quantity and types of microorganisms in the fermentation broth, the concentration and types of metabolites, and substrate consumption. This provides a scientific basis for optimizing and adjusting the fermentation process, such as rationally controlling operating conditions like fermentation temperature, pH, dissolved oxygen levels, and feed rate.

[0004] However, the sampling methods in the existing technology use manual sampling, which requires frequent interruptions to the fermentation process and increases the risk of contamination. Utility Model Content

[0005] The purpose of this invention is to provide an automatic sampling device for fermenters, which aims to solve the technical problem that the sampling method in the prior art uses manual sampling, which requires frequent interruption of the fermentation process and increases the risk of bacterial contamination.

[0006] To achieve the above objectives, this utility model employs an automatic sampling device for a fermenter, comprising a sampling tube, an electric diaphragm valve, a peristaltic pump, a connecting pipe, a controller, an air intake assembly, and multiple sampling assemblies. The sampling tube is placed inside the fermenter to be sampled. One end of the connecting pipe is connected to the sampling tube via the peristaltic pump. The electric diaphragm valve is mounted on the sampling tube. The air intake assembly and multiple sampling assemblies are all connected to the connecting pipe. The controller is connected to the electric diaphragm valve, the peristaltic pump, the air intake assembly, and the multiple sampling assemblies.

[0007] The air intake assembly includes an air intake pipe and an air clamp valve. The air intake pipe is connected to the connecting pipe, and the air clamp valve is disposed on the air intake pipe.

[0008] The air intake assembly further includes an air sterilization filter, which is disposed on the air intake pipe.

[0009] The sampling assembly includes a silicone tube, a quick connector, and a sampling bottle. One end of the silicone tube is connected to the connecting tube, and a discharge clamp valve is provided on the silicone tube. The sampling bottle is connected to the silicone tube through the quick connector.

[0010] The automatic sampling device for the fermentation tank also includes a water bath with a cooling function, which is used to hold multiple sampling bottles.

[0011] This utility model discloses an automatic sampling device for a fermenter. In practical use, the sampling cycle and sampling volume are pre-set in the controller. Upon reaching the first sampling time, the electric diaphragm valve opens, and compressed air is blown through the air inlet assembly to the sampling tube, expelling the fermentation liquid inside to ensure the sample represents the actual condition at that time. After the set time is reached, the air inlet assembly closes, the clamp valve corresponding to one of the sampling bottles opens, and the peristaltic pump starts. Under the action of the peristaltic pump, the fermentation liquid in the fermenter flows along the sampling tube, the electric diaphragm valve, the connecting pipe, and the discharge clamp valve into the corresponding sampling tube. Inside the bottle, the peristaltic pump simultaneously calculates the cumulative value. Once the peristaltic pump reaches the set value, the electric diaphragm valve closes, the air intake assembly opens, and the fermentation liquid from the connecting tube is pumped into the corresponding sampling bottle. Then, the discharge clamp valve closes, the electric diaphragm valve opens, and the air intake assembly pumps the fermentation liquid from the sampling tube back into the fermenter. This process completes sampling while simultaneously emptying the entire sampling device to avoid interference with subsequent sampling. This cycle continues until multiple sampling bottles have been sampled. This method solves the technical problem in existing technologies where manual sampling requires frequent interruptions to the fermentation process, increasing the risk of contamination. Attached Figure Description

[0012] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a schematic diagram of the structure of the automatic sampling device for fermentation tanks of this utility model.

[0014] 1-Sampling tube, 2-Electric diaphragm valve, 3-Peristaltic pump, 4-Connecting tube, 5-Controller, 6-Silicone tube, 7-Quick connector, 8-Sampling bottle, 9-Discharge clamp valve, 10-Air inlet pipe, 11-Air clamp valve, 12-Air sterilization filter, 13-Water bath with cooling function, 14-Fermentation tank. Detailed Implementation

[0015] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0016] Please see Figure 1 , Figure 1 This is a schematic diagram of the automatic sampling device for the fermenter 14 of this utility model.

[0017] This utility model provides an automatic sampling device for a fermenter 14, including a sampling tube 1, an electric diaphragm valve 2, a peristaltic pump 3, a connecting pipe 4, a controller 5, an air intake assembly, and multiple sampling assemblies. The sampling tube 1 is placed inside the fermenter 14 to be sampled. One end of the connecting pipe 4 is connected to the sampling tube 1 through the peristaltic pump 3. The electric diaphragm valve 2 is disposed on the sampling tube 1. The air intake assembly and multiple sampling assemblies are all connected to the connecting pipe 4. The controller 5 is connected to the electric diaphragm valve 2, the peristaltic pump 3, the air intake assembly, and multiple sampling assemblies.

[0018] The sampling assembly includes a silicone tube 6, a quick connector 7, and a sampling bottle 8. One end of the silicone tube 6 is connected to the connecting pipe 4. A discharge clamp valve 9 is provided on the silicone tube 6. The sampling bottle 8 is connected to the silicone tube 6 through the quick connector 7.

[0019] In this specific embodiment, during actual use, the sampling cycle and sampling volume are pre-set in the controller 5. Upon reaching the first sampling time, the electric diaphragm valve 2 opens, and compressed air is blown through the air intake assembly to the sampling tube 1, expelling the fermentation broth inside, ensuring that the sample represents the actual condition at that time. After the set time is reached, the air intake assembly closes, the clamp valve corresponding to one of the sampling bottles 8 opens, and the peristaltic pump 3 starts. Under the action of the peristaltic pump 3, the fermentation broth in the fermentation tank 14 flows along the sampling tube 1, the electric diaphragm valve 2, the connecting pipe 4, and the discharge clamp valve 9 into the corresponding sampling bottle 8. Simultaneously, the peristaltic pump 3 calculates the cumulative value; after the peristaltic pump 3 accumulates to the set value, the electric diaphragm valve 2 closes, the air intake component opens, and the fermentation liquid from the connecting pipe 4 is pumped into the corresponding sampling bottle 8. Then, the discharge clamp valve 9 closes, the electric diaphragm valve 2 opens, and the air intake component pumps the fermentation liquid in the sampling pipe 1 back into the fermentation tank 14. In this way, while completing the sampling, the fermentation liquid in the entire sampling device is emptied to avoid interference with the next sampling. This cycle continues until multiple sampling bottles 8 have been sampled. This method solves the technical problem in the prior art where manual sampling is used, which requires frequent interruptions of the fermentation process and increases the risk of contamination.

[0020] The air intake assembly includes an air intake pipe 10 and an air clamp valve 11. The air intake pipe 10 is connected to the connecting pipe 4, and the air clamp valve 11 is disposed on the air intake pipe 10.

[0021] In this specific embodiment, the air inlet pipe 10 is used to supply air to the sampling pipe 1 and the connecting pipe 4, and the air clamp valve 11 is used to control the opening and closing of the air inlet pipe 10.

[0022] Secondly, the air intake assembly also includes an air sterilization filter 12, which is disposed on the air intake pipe 10.

[0023] In this specific embodiment, the air sterilization filter 12 is used to filter and sterilize the gas entering the sampling tube 1 and the connecting tube 4.

[0024] Meanwhile, the automatic sampling device of the fermenter 14 also includes a water bath 13 with a cooling function, which is used to place multiple sampling bottles 8.

[0025] In this specific embodiment, the water bath 13 with cooling function is used to place multiple sampling bottles 8 and always keeps the sample below 4°C, which can ensure the integrity of the sample during the storage period.

[0026] Using the automatic sampling device for a fermenter 14 according to this utility model, in specific use, the sampling cycle and sampling volume are preset in the controller 5. Upon reaching the first sampling time, the electric diaphragm valve 2 opens, and compressed air is blown through the air inlet assembly to the sampling tube 1, expelling the fermentation liquid inside to ensure that the sample represents the actual situation at that time. After the set time is reached, the air inlet assembly closes, the clamp valve corresponding to one of the sampling bottles 8 opens, and the peristaltic pump 3 starts. Under the action of the peristaltic pump 3, the fermentation liquid in the fermenter 14 flows along the sampling tube 1, the electric diaphragm valve 2, the connecting pipe 4, and the discharge clamp valve 9 into the corresponding [sampling tube 14]. While sampling bottle 8 is being sampled, peristaltic pump 3 calculates the cumulative value. Once the cumulative value of peristaltic pump 3 reaches the set value, electric diaphragm valve 2 closes, air intake assembly opens, and the fermentation liquid from connecting pipe 4 is pumped into the corresponding sampling bottle 8. Then, discharge clamp valve 9 closes, electric diaphragm valve 2 opens, and air intake assembly pumps the fermentation liquid in sampling pipe 1 back into fermentation tank 14. In this way, while sampling is completed, the fermentation liquid in the entire sampling device is emptied to avoid interference with the next sampling. This cycle continues until multiple sampling bottles 8 have been sampled. This method solves the technical problem in the prior art where manual sampling is required, which necessitates frequent interruptions of the fermentation process and increases the risk of contamination.

[0027] The above-disclosed embodiments are merely preferred embodiments of the present utility model and should not be construed as limiting the scope of the present utility model. Those skilled in the art can understand that implementing all or part of the above-described embodiments and making equivalent changes in accordance with the claims of the present utility model are still within the scope of the utility model.

Claims

1. An automatic sampling device for a fermenter, characterized in that, The device includes a sampling tube, an electric diaphragm valve, a peristaltic pump, a connecting tube, a controller, an air intake assembly, and multiple sampling assemblies. The sampling tube is placed inside the fermenter to be sampled. One end of the connecting tube is connected to the sampling tube via the peristaltic pump. The electric diaphragm valve is mounted on the sampling tube. The air intake assembly and multiple sampling assemblies are all connected to the connecting tube. The controller is connected to the electric diaphragm valve, the peristaltic pump, the air intake assembly, and the multiple sampling assemblies.

2. The automatic sampling device for fermenters as described in claim 1, characterized in that, The air intake assembly includes an air intake pipe and an air clamp valve. The air intake pipe is connected to the connecting pipe, and the air clamp valve is disposed on the air intake pipe.

3. The automatic sampling device for fermenters as described in claim 2, characterized in that, The air intake assembly also includes an air sterilization filter, which is disposed on the air intake pipe.

4. The automatic sampling device for fermenters as described in claim 3, characterized in that, The sampling assembly includes a silicone tube, a quick connector, and a sampling bottle. One end of the silicone tube is connected to the connecting tube, and a discharge clamp valve is provided on the silicone tube. The sampling bottle is connected to the silicone tube through the quick connector.

5. The automatic sampling device for fermenters as described in claim 4, characterized in that, The automatic sampling device for the fermenter also includes a water bath with a cooling function, which is used to hold multiple sampling bottles.

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