High-throughput culture device for identifying penetrating power of plant root system

Abstract

The utility model relates to the technical field of plant root box test equipment, and discloses a high-flux culture device for identifying the penetrating power of a plant root system, which comprises a lower box body with an open top, and a plurality of first growth units separated in the lower box body; the supporting net is horizontally arranged at the top of the lower box body; the wax sheet is horizontally arranged at the top of the supporting net; the top and the bottom of the upper box body are open, the upper box body is arranged on the lower box body, and the upper box body is divided into a plurality of second growth units corresponding to the first growth units; the first growth unit and the second growth unit are used for being filled with a growth medium; the device system can be used for accurately measuring the penetrating power of a plant root system and quantitatively analyzing the morphological configuration and the penetrating power of the root system, is suitable for batch screening of multiple varieties of crops, can be used for researching adversity stress response of the root system to soil mechanical compaction, moisture, nutrients and the like, meets and adapts to the requirements of agricultural production operation, and has a wide application prospect. The purposes of scientific planting and crop yield guaranteeing are achieved.

Description

Technical Field

[0001] This utility model relates to the technical field of plant root box test equipment, and in particular to a high-throughput culture device for identifying the penetration ability of plant roots. Background Technology

[0002] In recent years, with the promotion and application of reduced-till and no-till technologies, the improvement of agricultural mechanization, and the increase in the number and load capacity of agricultural machinery, farmland soil compaction has become increasingly severe, and soil compaction has become more and more common. Compaction affects soil structure, increases soil mechanical resistance, hinders root growth, reduces root quantity, alters root shape and growth direction, and limits root access to soil water and nutrients. Penetration capacity is an indicator of a plant root's ability to penetrate the soil, and this capacity decreases with increasing soil compaction. Numerous studies have shown that crop varieties with different root traits have varying degrees of adaptability to environmental stress. Therefore, optimizing root traits and functions, and breeding superior crop varieties with different root penetration capabilities, are particularly important to meet the current needs of agricultural production.

[0003] Traditional root research methods, such as digging, root drilling, and root windowing, are time-consuming and labor-intensive, and cause significant damage to root structure and morphology during sample processing, leading to substantial errors in the results. Furthermore, the inability to obtain complete plant root samples severely limits the development of root research. In indoor research, methods such as hydroponics, aeroponics, and net bags are commonly used. While these methods can obtain complete root samples compared to traditional field methods, the morphological characteristics of plant roots grown under these conditions differ greatly from their growth in soil, failing to accurately reflect the plant's growth status in the soil. Therefore, the research results deviate somewhat from actual field measurements and applications. Utility Model Content

[0004] This invention proposes a high-throughput culture device for identifying the root penetration ability of plants, overcoming the shortcomings of the prior art. This high-throughput culture device, while ensuring authentic plant growth, can rapidly obtain complete root samples and accurately measure root penetration ability, making it suitable for batch screening of multiple crop varieties. Simultaneously, this high-throughput culture device can also be used to study root responses to abiotic stresses such as soil compaction, water, and nutrients, meeting and adapting to the needs of agricultural production operations, achieving the goals of scientific planting and ensuring crop yield.

[0005] The technical solution of this utility model is: a high-throughput culture device for identifying the root penetration ability of plants, comprising:

[0006] The lower housing is open at the top, and the lower housing is divided into multiple first growth units;

[0007] The support netting is horizontally installed at the top of the lower housing.

[0008] Wax sheets are horizontally positioned on top of the support mesh;

[0009] The upper chamber is open at the top and bottom and is set on the lower chamber. The upper chamber is divided into multiple second growth units corresponding to the first growth unit. The first and second growth units are used to fill the growth medium.

[0010] In at least one embodiment of this utility model, the wax sheet is made of paraffin wax and petroleum jelly.

[0011] In at least one embodiment of the present invention, a plurality of first growth units and a plurality of second growth units are each fitted with a top-and-bottom open PE plastic bag, the size of which is adapted to the inner cavity of the first and second growth units; the PE plastic bag is used to fill the growth medium.

[0012] In at least one embodiment of the present invention, the growth medium includes: soil, sand or agar.

[0013] In at least one embodiment of the present invention, a plurality of longitudinal slots are evenly provided on the inner walls of the upper box and the lower box, and a partition fence is engaged in the slots. The plurality of partition fences are arranged orthogonally to form a plurality of first growth units and a plurality of second growth units.

[0014] In at least one embodiment of the present invention, the upper port of the lower housing is a boss-shaped structure, and the support mesh and wax sheet are both fastened to the upper port of the lower housing.

[0015] In at least one embodiment of the present invention, a fastener seat is provided on the outer side wall of the upper box, and a movable fastener that cooperates with the fastener seat is provided on the outer side wall of the lower box; the fastener seat is used to receive the movable fastener so that the upper box and the lower box are locked together.

[0016] In at least one embodiment of the present invention, the bottom of the lower housing has a plurality of drainage holes, which are evenly arranged.

[0017] This invention enables the application of a high-throughput culture device for identifying the root penetration ability of multi-genotype roots in root trait analysis, dynamic observation, identification, screening, and its resistance to water, nutrients, salt, alkali, and heavy metal stress.

[0018] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0019] 1. This utility model comprises a lower box internally divided into multiple first growth units, a support net and wax sheets mounted on the lower box, and an upper box internally divided into multiple second growth units mounted on the lower box. In use, for different plants, a suitable growth medium is first added to the multiple first growth units in the lower box. Then, the support net is installed, and wax sheets of appropriate thickness are placed on the support net. The upper box is then placed, and growth medium is added to the upper box before planting. When the plants are harvested and root sampling and testing are required in the upper and lower boxes, the roots in both boxes can be cut off with a knife to separate the upper and lower boxes. Complete root samples can then be extracted from the first and second growth units. Compared with existing technologies, this high-throughput cultivation device can quickly obtain complete root samples while ensuring the actual growth of the plants, accurately measuring the root penetration ability of plants, and ensuring the accuracy of research results in field applications.

[0020] 2. This invention features a detachable first and second growth unit, formed by interlocking gratings, within the upper and lower housings. During plant cultivation, the growth medium is filled into a PE plastic bag. When sampling the plant roots, the first and second growth units can be quickly disassembled, and the plant roots can be rapidly extracted to obtain high-quality root trait parameters. This device provides an efficient and accurate research platform for studying the genetic diversity of root traits and simultaneously breeding varieties with different root penetration abilities.

[0021] 3. This utility model has a wide range of applications. It can be used to conduct research on the root penetration ability of a large number of plant genotypes and varieties in a relatively small space. It is particularly suitable for the analysis and screening of root penetration ability traits of multiple different genotypes or varieties of the same crop. At the same time, it can be used to study the root system's response to soil mechanical compaction, water, nutrients and other abiotic stresses, so as to meet and adapt to the needs of agricultural production operations and achieve the goal of scientific planting and ensuring crop yield. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the device structure of this utility model.

[0023] Figure 2 This is a schematic diagram of the assembly of the device of this utility model.

[0024] Figure 3 This is a top view of the upper housing of this utility model.

[0025] Figure 4 This is a schematic diagram of the fence of this utility model.

[0026] Explanation of reference numerals in the attached figures:

[0027] 1. Lower box; 11. First growth unit; 12. Card slot; 13. Fence; 14. Drainage hole; 2. Support net; 3. Wax sheet; 4. Upper box; 41. Second growth unit; 5. Fastener; 51. Movable buckle; 6. PE plastic bag. Detailed Implementation

[0028] The accompanying drawings in this invention are not strictly drawn to scale; the specific dimensions and quantity of each structure can be determined according to actual needs. The drawings described in this invention are merely structural schematic diagrams.

[0029] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the described embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0030] Unless otherwise defined, the technical or scientific terms used herein shall have the ordinary meaning understood by one of ordinary skill in the art to which this invention pertains. The terms "first," "second," and similar terms used in this invention do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as "comprising" or "including" mean that the element or object preceding the word encompasses the elements or objects listed following the word and their equivalents, without excluding other elements or objects. Terms such as "inner," "outer," "upper," "lower," "far," "near," "front," and "rear" are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0031] Combination Figures 1 to 4 As shown, a high-throughput culture device for identifying the root penetration ability of plants includes:

[0032] The top of the lower box 1 is open, and the lower box 1 is divided into multiple first growth units 11.

[0033] The support net 2 is horizontally set at the top of the lower box 1; the support net 2 is only used to support the wax sheet 3, and the mesh setting of the support net 2 should not affect the plant roots from passing through.

[0034] Wax sheet 3 is horizontally positioned on top of support net 2.

[0035] The upper box 4 is open at the top and bottom and is set on the lower box 1. The upper box 4 is divided into multiple second growth units 41 corresponding to the first growth unit 11. The first growth unit 11 and the second growth unit 41 are used to fill the growth medium.

[0036] As an alternative embodiment, wax sheet 3 is made of paraffin wax and petroleum jelly in a specific ratio; specifically, the wax sheet is made of paraffin wax and petroleum jelly in a certain ratio by weight according to the needs of the test.

[0037] As an alternative embodiment, multiple first growth units 11 and multiple second growth units 41 are each fitted with a top-and-bottom open PE plastic bag 6, the size of which is adapted to the inner cavity of the first growth unit 11 and the second growth unit 41; the PE plastic bag 6 is used to fill the growth medium.

[0038] As an alternative embodiment, the growth medium includes soil, sand, or agar.

[0039] As an alternative embodiment, multiple longitudinal slots 12 are evenly provided on the inner walls of the upper box 4 and the lower box 1. A grid 13 is engaged in the slot 12. The multiple grids 13 are arranged orthogonally to form multiple first growth units 11 and multiple second growth units 41. After placing wax sheets 3 of different hardness on the support net 2, the gap between the wax sheets 3 and the lower box 1 is filled with sealant. The upper box 4 is then fastened onto the lower box 1, and the gap between the wax sheet material and the upper box 4 and the grid 13 is filled with sealant. The grid 13 is composed of 2mm thick plastic plates. The grid 13 is cut at half its height, and the four cut grid plates 13 are staggered along the cut to form a culture unit.

[0040] As an alternative embodiment, the upper port of the lower housing 1 is a boss-shaped structure, and the support mesh 2 and wax sheet 3 are both snapped onto the upper port of the lower housing; the upper housing 4 is fastened to the lower housing 1, making the connection more stable.

[0041] As an alternative embodiment, the outer side wall of the upper box 4 is provided with a fastener seat 5, and the outer side wall of the lower box 1 is provided with a movable fastener 51 that cooperates with the fastener seat 5. The fastener seat 5 is used to receive the movable fastener 51 so that the upper box 4 and the lower box 1 are locked together. The movable fastener 51 fixes the lower box 1 and the upper box 4 by pressing and locking, and uses a locking mechanism to ensure the safety and stability of the device. After the plant is cultivated, the fastener is fixed. When it is necessary to sample and test the root system in the upper box 4 and the lower box 1 when the plant is harvested, the fastener only needs to be opened and the root system of the upper and lower boxes 1 can be cut with a knife to separate the upper box 4 and the lower box 1.

[0042] As an alternative embodiment, the bottom of the lower box 1 has multiple drainage holes 14, which are evenly arranged. When used for plant cultivation, excess water in the lower box 1 can be drained through the drainage holes 14, while also allowing the cultivated plants to be ventilated.

[0043] As an alternative embodiment, four brake casters are fixed to the bottom of the lower housing 1 to facilitate the movement and braking of the device, ensuring the device's mobility, stability and safety.

[0044] This invention enables the application of a high-throughput culture device for identifying the root penetration ability of multi-genotype roots in root trait analysis, dynamic observation, identification, screening, and its resistance to water, nutrients, salt, alkali, and heavy metal stress.

[0045] Specific implementation examples of this utility model:

[0046] Conduct research on root penetration ability of maize with multiple genotypes (varieties). Select maize varieties with different genotypes in my country to study the diversity of maize root traits and root penetration ability. Based on the collected root data, conduct comprehensive analysis to identify representative varieties with different root penetration abilities and their root trait characteristics. This data will be used to study the response of maize varieties with different root penetration abilities to different soil compaction stresses.

[0047] like Figure 1 As shown, a support net 2 is installed on the lower box 1 for placing anti-root penetration wax sheets 3. Multiple evenly arranged slots 12 are installed on the inner side wall of the lower box 1. The multiple slots 12 extend through the inner side wall of the lower box 1 to the bottom of the lower box 1 for engaging the partition fence 13. Four partition fences 13 form the first growth unit 11, and a PE plastic bag 6 with the same length and width as the first growth unit 11 is placed there.

[0048] The upper box 4 and the lower box 1 used in conjunction with the upper box 4 are provided. Multiple slots 12 are installed on the inner side wall of the upper box 4, extending through the inner side wall of the upper box 4 to the lower part of the upper box 4, for engaging the partition fence 13. One side of the partition fence 13 is connected to the slot 12 inside the upper box 4, and the other side is engaged with the slot 12 at the corresponding position on the opposite side of the upper box 4. The four partition fences 13 form the second growth unit 41, and a PE plastic bag 6 with the same length and width as the second growth unit 41 is placed inside.

[0049] like Figure 2 As shown, when the high-throughput root penetration device is used to conduct plant root penetration tests, the support plate is a mesh support net 2, and a 4mm thick wax sheet 3 is placed on it. The wax sheet 3 is mixed with paraffin wax and petroleum jelly in a ratio of 6:4 (by weight). The gap between the wax sheet 3 and the lower box 2 is filled with sealant. The upper box 4 is then placed on the lower box 1, and the gap between the wax sheet 3 and the upper box 4, as well as the partition 13, is filled with sealant.

[0050] like Figure 1 As shown, the upper box 4 and lower box 1 are fixed by multiple fasteners 5 and movable buckles 51 to ensure the safety and stability of the device. After the plant is cultivated, the fasteners are fixed. When it is necessary to sample and test the root system in the upper box 4 and lower box 1 during the harvest, simply open the movable buckle 51 and cut the root system of the upper box 4 and lower box 1 with a knife to separate the upper box 4 and lower box 1.

[0051] In the plant root penetration ability experiment, the growth medium within the growth unit was soil. The soil bulk density was set at 1.2 g / cm³. -3 Each growing unit was filled with 1.75 kg of dry soil. Before filling, the fertilizer was thoroughly mixed with the soil. The fertilizers were urea, superphosphate, and potassium chloride, with dosages of N: 250 mg / kg. -1 Soil, P2O5: 250mg kg -1 Soil, K2O: 150 mg / kg -1 Soil. Uniformly sized corn seeds of each tested variety were disinfected with a 10% H₂O₂ solution for 30 minutes, rinsed thoroughly with distilled water, and then soaked overnight in saturated CaSO₄. The soaked seeds were sown into the growing units, three seeds per variety. Seven days after emergence, seedlings were thinned to one plant per seedling. A handheld soil moisture meter was used to regularly measure soil moisture content, maintaining it at 70% of field capacity. The location and orientation of the growing boxes were periodically changed to minimize the impact of the greenhouse environment on corn growth.

[0052] The above embodiments are merely specific implementations of this utility model patent, used to illustrate the technical solution of this utility model patent, and not to limit it. The protection scope of this utility model patent is not limited thereto. Although this utility model patent has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that any person skilled in the art can still modify or easily conceive of changes to the technical solutions described in the foregoing embodiments, or make equivalent substitutions for some of the technical features within the technical scope disclosed in this utility model; and these modifications, changes, or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions implemented by this utility model patent, and should all be covered within the protection scope of this utility model.

Claims

1. A high-throughput culture device for identifying the root penetration ability of plants, characterized in that, include: The lower box (1) is open at the top, and the lower box (1) is divided into multiple first growth units (11). Support net (2) is horizontally set on top of the lower box (1); Wax sheet (3) is horizontally set on top of support net (2); The upper box (4) is open at the top and bottom. The upper box (4) is set on the lower box (1). The upper box (4) is divided into multiple second growth units (41) corresponding to the first growth unit (11). The first growth unit (11) and the second growth unit (41) are used to fill the growth medium.

2. The high-throughput culture device for identifying the root penetration ability of plants as described in claim 1, characterized in that, Each of the first growth units (11) and the second growth units (41) is fitted with a top-and-bottom open PE plastic bag (6), the size of which is adapted to the inner cavity of the first growth unit (11) and the second growth unit (41); the PE plastic bag (6) is used to fill the growth medium.

3. The high-throughput culture device for identifying the root penetration ability of plants as described in claim 1, characterized in that, The growth medium includes soil, sand, or agar.

4. The high-throughput culture device for identifying the root penetration ability of plants as described in claim 1, characterized in that, Multiple longitudinal slots (12) are evenly provided on the inner walls of the upper box (4) and the lower box (1). A partition fence (13) is engaged in the slot (12). The multiple partition fences (13) are arranged orthogonally to form multiple first growth units (11) and multiple second growth units (41).

5. The high-throughput culture device for identifying the root penetration ability of plants as described in claim 1, characterized in that, The upper port of the lower box (1) is in the shape of a boss, and the support net (2) and wax sheet (3) are both fastened to the upper port of the lower box.

6. The high-throughput culture device for identifying plant root penetration ability as described in claim 1, characterized in that, The upper box (4) is provided with a buckle seat (5) on its outer side wall, and the lower box (1) is provided with a movable buckle (51) that cooperates with the buckle seat (5) on its outer side wall; the buckle seat (5) is used to receive the movable buckle (51) so that the upper box (4) and the lower box (1) are locked together.

7. The high-throughput culture device for identifying plant root penetration ability as described in claim 1, characterized in that, The bottom of the lower housing (1) has multiple drainage holes (14), which are evenly arranged.

Images