Stem cell cryopreservation tube

Abstract

The utility model discloses a stem cell cryopreservation tube, and belongs to the technical field of cryopreservation tubes. Comprising an outer tube; the sealing cover is used for sealing the top opening of the outer pipe; the movable rod is connected to the bottom of the sealing cover through a connecting piece, and the vertex of the movable rod rotates around a shaft along the axis of the sealing cover; the balancing weight is fixed at the bottom of the movable rod; the inner wall of the outer pipe is provided with a spiral flow channel which is spirally downward; a liquid inlet and a liquid outlet which are communicated with the top and the bottom of the spiral flow channel are formed in the top and the bottom of the outer wall of the outer pipe respectively, and the outer wall of the inner pipe is attached to the inner wall of the outer pipe; a circle of the top of the inner pipe is provided with a second limit used for clamping the top of the outer pipe. The utility model provides a stem cell cryopreservation tube which accelerates unfreezing of stem cells in a hand-cranking mode and is simple in structure and low in cost.

Description

Technical Field

[0001] This utility model relates to the technical field of cryopreservation tubes, and more specifically, to a stem cell cryopreservation tube. Background Technology

[0002] Cryopreservation tubes are specialized containers used in laboratories for the cryopreservation of biological samples.

[0003] Patent application number 202121014223.5 discloses a cryopreservation tube for mesenchymal stem cells, including a tube body, a rotating rod inside the tube body, a spiral stirring blade on the rotating rod, the lower end of the rotating rod being rotatably connected to the inside of the tube body via a bearing, and a tube cap being threaded to the top end of the tube body.

[0004] The aforementioned patent uses a motor and gears to drive a stirring plate on a rotating rod to accelerate the thawing of cells in cryopreservation tubes during cell thawing. However, the structure is too complex, requires electrical operation, and is cumbersome to operate. Utility Model Content

[0005] To overcome the technical problems in the prior art described above, this invention provides a stem cell cryopreservation tube.

[0006] To solve the above-mentioned technical problems, the technical solution of this utility model is as follows:

[0007] A stem cell cryopreservation tube, comprising:

[0008] outer tube;

[0009] A cap is used to seal the top opening of the outer tube;

[0010] The movable rod is connected to the bottom of the cover via a connector, and the apex of the movable rod rotates around the axis of the cover.

[0011] The counterweight is fixed to the bottom of the movable rod.

[0012] During the thawing process, shaking allows the movable rod to stir the cell fluid in the outer tube, and the counterweight increases its weight during this process.

[0013] Preferably, the outer tube is made of polypropylene.

[0014] Preferably, the connector is a nylon rope or a universal joint.

[0015] Preferably, the universal joint is a hook.

[0016] Preferably, the cap and the top of the outer tube are threaded together.

[0017] Preferably, the inner wall of the outer tube is provided with a downward spiral flow channel; the top and bottom of the outer wall of the outer tube are respectively provided with an inlet and an outlet communicating with the top and bottom of the spiral flow channel, wherein,

[0018] It also includes an inner tube, the outer wall of which is fitted together with the inner wall of the outer tube; a second limiting device is provided around the top of the inner tube for engaging the top of the outer tube.

[0019] Preferably, there is a gap between the bottom of the inner tube and the bottom of the inner wall of the outer tube; the spiral flow channel communicates with the gap.

[0020] Preferably, a first limit is provided on the upper part of the inner wall of the inner tube, and a limit ring is detachably installed on the first limit. A limit hole is provided in the middle of the limit ring, and the movable rod passes through the limit hole.

[0021] The swing amplitude of the movable rod is limited by a limiting hole in the middle of the limiting ring to prevent the configuration block from colliding with the pipe wall.

[0022] Preferably, an ultrafiltration membrane is provided at the bottom of the limiting ring, the top opening of the ultrafiltration membrane is fixed to the bottom of the limiting ring, the limiting hole communicates with the interior of the ultrafiltration membrane, and the counterweight is located inside the ultrafiltration membrane.

[0023] Filtration and purification are achieved by setting up an ultrafiltration membrane.

[0024] Preferably, the ultrafiltration membrane material is an organic polymer membrane.

[0025] Compared with the prior art, the beneficial effects of this utility model's technical solution are:

[0026] This invention provides a stem cell cryopreservation tube, in which a movable rod is installed inside and a weight is applied to its bottom. The movable rod is manually cranked to agitate the stem cell fluid, thereby accelerating the thawing process. Specifically, it also includes:

[0027] 1) Limiting ring; The limiting hole in the middle of the limiting ring can reduce the swing amplitude of the moving rod. Through reasonable size setting, it can prevent the counterweight from hitting the tube wall during the shaking process.

[0028] 2) Ultrafiltration membrane; Ultrafiltration membranes trap macromolecules, colloids, particles and bacteria in the upper part for purification.

[0029] 3) Set up an outer tube and an inner tube combination, and set up a spiral flow channel, liquid inlet and liquid outlet; if the outer tube is placed directly in the cryopreservation solution such as liquid nitrogen, the stem cell cryopreservation effect in the tube may be inconsistent due to insufficient liquid level. The cryopreservation solution is circulated using a spiral flow channel to ensure that the stem cell cryopreservation effect in all positions in the tube is consistent. Attached Figure Description

[0030] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0031] Figure 1 This is an exploded structural diagram of Embodiment 1 of the present invention;

[0032] Figure 2 This is an exploded structural diagram of Embodiment 2 of the present invention;

[0033] Figure 3 This is a schematic diagram of the structure of Embodiment 3 of this utility model;

[0034] Figure 4 This is a schematic diagram of the internal cross-sectional structure of Embodiment 3 of this utility model.

[0035] The markings in the diagram are as follows: 1. Outer tube; 2. Inner tube; 3. Cap; 4. Movable rod; 5. Counterweight; 6. Limiting ring; 7. Ultrafiltration membrane; 8. First limit; 9. Second limit; 10. Spiral flow channel; 11. Liquid inlet; 12. Liquid outlet; 13. Nylon rope. Detailed Implementation

[0036] To better understand the purpose, structure, and function of this utility model, the technical solution of this utility model will be further described in detail below with reference to the accompanying drawings and specific preferred embodiments.

[0037] In the description of this utility model, it should be understood that the terms "left side," "right side," "upper part," "lower part," etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. "First," "second," etc., do not indicate the importance of the components, and therefore should not be construed as a limitation of this utility model. The specific dimensions used in the embodiments are only for illustrating the technical solution and do not limit the scope of protection of this utility model. It is understandable that some well-known structures and their descriptions may be omitted in the accompanying drawings for those skilled in the art.

[0038] Unless otherwise expressly specified and limited, the terms "installation," "setting," "connection," and "fixation" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0039] Example 1:

[0040] like Figure 1 and Figure 4 As shown, this application provides a stem cell cryopreservation tube, comprising: an outer tube 1; a cap 3 for sealing the top opening of the outer tube 1; a movable rod 4 connected to the bottom of the cap 3 via a connector, and the apex of the movable rod 4 rotating around the axis of the cap 3; and a counterweight 5 fixed to the bottom of the movable rod 4.

[0041] Specifically, the connector is a nylon rope 13 or a universal joint.

[0042] Furthermore, the universal joint can be a hook. For example, a vertical ring can be fixed at the bottom of the cover 3. The ring can be connected to the bottom of the cover 3 by a swivel joint. Then, a hook can be set on the top of the movable rod 4 and fitted onto the ring. This can achieve the swing direction requirement of the movable rod 4. Alternatively, a ball bearing universal joint can be used to achieve the same effect.

[0043] Specifically, such as Figure 1 As shown, for easy disassembly, the cap 3 and the top of the outer tube 1 are threaded together.

[0044] In the above embodiments, when thawing is required, the outer tube 1 is shaken, and under the action of the counterweight 5, the movable rod 4 can swing in the outer tube 1 through the nylon rope 13, thereby accelerating thawing by stirring.

[0045] Example 2:

[0046] The difference from the above embodiments is that, based on the above embodiments, as follows: Figure 2 As shown, the inner wall of the outer tube 1 is provided with a spiral flow channel 10 that spirals downwards; the top and bottom of the outer wall of the outer tube 1 are respectively provided with an inlet 11 and an outlet 12 communicating with the top and bottom of the spiral flow channel 10, wherein,

[0047] It also includes an inner tube 2, the outer wall of the inner tube 2 and the inner wall of the outer tube 1 are fitted together; a second limiting 9 is provided around the top of the inner tube 2 for locking the top of the outer tube 1.

[0048] To ensure consistent stem cell cryopreservation results throughout the tube, stem cells are cryopreserved by circulating the cryopreservation solution within the spiral flow channel 10. This is done in conjunction with a circulation pump, allowing the cryopreservation solution, such as liquid nitrogen, to enter through the inlet 11, flow through the spiral flow channel 10, and exit through the outlet 12. Compared to directly immersing the cryopreservation tube in the cryopreservation solution, there is no need to consider whether the liquid level completely exceeds the height of the stem cell solution in the tube.

[0049] Furthermore, such as Figure 4 As shown, there is a gap between the bottom of the inner tube 2 and the bottom of the inner wall of the outer tube 1; the spiral flow channel 10 is connected to the gap. Considering that the bottom of the cryopreservation tube is usually circular, the spiral flow channel 10 is difficult to cover the entire inside of the tube. Therefore, there is a gap between the bottom of the inner tube 2 and the bottom of the inner wall of the outer tube 1 to ensure that the stem cell fluid at the bottom of the inner tube 2 can be effectively frozen.

[0050] Furthermore, such as Figure 2 As shown, a first limiter 8 is provided on the upper part of the inner wall of the inner tube 2. A limiter ring 6 is detachably installed on the first limiter 8. A limiter hole is provided in the middle of the limiter ring 6. The movable rod 4 passes through the limiter hole. The limiter hole in the middle of the limiter ring 6 can reduce the swing amplitude of the movable rod 4. Through reasonable size setting, the counterweight 5 is prevented from hitting the tube wall during the shaking process.

[0051] Example 3:

[0052] The difference from the above embodiments is that, based on the above embodiments, as follows: Figure 3 and Figure 4 As shown, an ultrafiltration membrane 7 is provided at the bottom of the limiting ring 6. The top opening of the ultrafiltration membrane 7 is fixed to the bottom of the limiting ring 6. The limiting hole communicates with the interior of the ultrafiltration membrane 7, and the counterweight 5 is located inside the ultrafiltration membrane 7.

[0053] More specifically, the ultrafiltration membrane material is an organic polymer membrane.

[0054] The ultrafiltration membrane 7 can trap macromolecules, colloids, particles and bacteria in the upper part for purification. When pouring out the thawed cell fluid, open the cap 3, take out the movable rod 4 and the counterweight 5, and then take out the limiting ring 6 and the ultrafiltration membrane 7. The purified stem cell fluid is left in the inner tube 2.

[0055] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating this utility model, and are not intended to limit the implementation of this utility model. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.

Claims

1. A stem cell cryopreservation tube, characterized in that, include: Outer tube (1); A cap (3) is used to seal the top opening of the outer tube (1); The movable rod (4) is connected to the bottom of the cover (3) via a connector, and the apex of the movable rod (4) rotates around the axis along the axis of the cover (3); The counterweight (5) is fixed to the bottom of the movable rod (4).

2. The stem cell cryopreservation tube according to claim 1, characterized in that, The connector is a nylon rope (13) or a universal joint.

3. The stem cell cryopreservation tube according to claim 2, characterized in that, The cap (3) and the outer tube (1) are threaded together at the top.

4. The stem cell cryopreservation tube according to claim 3, characterized in that, The inner wall of the outer tube (1) is provided with a spiral flow channel (10) that spirals downwards; the top and bottom of the outer wall of the outer tube (1) are respectively provided with an inlet (11) and an outlet (12) communicating with the top and bottom of the spiral flow channel (10), wherein, It also includes an inner tube (2), the outer wall of the inner tube (2) and the inner wall of the outer tube (1) are fitted together; a second limiter (9) is provided around the top of the inner tube (2) for engaging the top of the outer tube (1).

5. The stem cell cryopreservation tube according to claim 4, characterized in that, There is a gap between the bottom of the inner tube (2) and the bottom of the inner wall of the outer tube (1); the spiral flow channel (10) is connected to the gap.

6. The stem cell cryopreservation tube according to claim 5, characterized in that, The inner tube (2) has a first limit (8) on the upper part of its inner wall. A limit ring (6) is detachably installed on the first limit (8). A limit hole is provided in the middle of the limit ring (6), and the movable rod (4) passes through the limit hole.

7. The stem cell cryopreservation tube according to claim 6, characterized in that, The bottom of the limiting ring (6) is provided with an ultrafiltration membrane (7), and the top opening of the ultrafiltration membrane (7) is fixed to the bottom of the limiting ring (6). The limiting hole is connected to the inside of the ultrafiltration membrane (7), and the counterweight (5) is located inside the ultrafiltration membrane (7).

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