Metal bath capable of preventing cross contamination

Abstract

The utility model provides a cross contamination prevention metal bath which comprises a metal bath body and a heating template which is arranged in the metal bath body and is provided with a centrifugal tube hole site upwards, and a hemispherical cover plate is hinged to the upper portion of the metal bath body. The hemispherical cover plate is provided with a centrifugal tube plug pressure applying assembly which penetrates through and extends to the upper part of the hemispherical cover plate. According to the cross contamination prevention metal bath, the sample pressing plate is additionally arranged on the metal bath, the sample pressing plate can firmly press a centrifugal tube cover plug through adjustment of the lead screw on the nut, and the situation that the cover plug bounces off due to the fact that steam pressure in a tube is increased is effectively prevented. Therefore, small molecule DNA / RNA fragments in the positive sample cannot be splashed to form aerosol due to the fact that the cover plug is bounced off, and other negative samples cannot be polluted, so that false positive results of negative sample detection results are avoided, the accuracy and reliability of DNA / RNA detection are greatly improved, it is ensured that the experimental results truly reflect sample conditions, and the accuracy and reliability of DNA / RNA detection are improved. And an accurate basis is provided for medical diagnosis.

Description

Technical Field

[0001] This utility model relates to the field of medical equipment technology, specifically to a metal bath that prevents cross-contamination. Background Technology

[0002] DNA / RNA are very light molecules. In the process of testing a certain DNA / RNA in a sample, DNA / RNA needs to be extracted manually. Centrifuge tubes with snap-on caps are used in the extraction process, and high-temperature incubation equipment is used in the manual operation.

[0003] Currently, the metal bath equipment used in laboratories only has heating devices and lacks sample protection devices. The temperature is usually set at 100℃. When centrifuge tubes containing extracts and samples are incubated, the caps of the centrifuge tubes may pop open due to the increased vapor pressure inside the tubes. During this process, small DNA / RNA fragments from very light positive samples may splash out, forming aerosols that contaminate other negative samples that have been exposed due to vapor pressure. This can lead to false positives in the test results of negative samples and increase the risk of aerosol contamination in the laboratory. Summary of the Invention

[0004] To address the shortcomings of existing equipment, this invention adds a sample pressure plate. During centrifuge tube incubation, external force is used to press down the cap, preventing the centrifuge tube cap from popping open due to vapor pressure. This avoids cross-contamination of DNA / RNA during extraction and reduces the risk of aerosol contamination in the laboratory.

[0005] The technical solution of this utility model is implemented as follows:

[0006] A cross-contamination-preventing metal bath includes a metal bath body and a heating template with upward-facing centrifuge tube openings disposed inside it. A hemispherical cover plate is hinged above the metal bath body. A centrifuge tube plug pressure assembly is provided on the hemispherical cover plate, extending through it and above it. The centrifuge tube plug pressure assembly includes a sample pressure plate at the bottom for abutting against the centrifuge tube plug and a lead screw disposed above the sample pressure plate. The lead screw is threaded into a nut disposed on the hemispherical cover plate and extends above the hemispherical cover plate. Force is applied to the lead screw to make it feed linearly and drive the sample pressure plate to rise or fall.

[0007] Furthermore, the sample pressure plate is a circular plate with an arc-shaped slope at its outer edge that fits the inner wall of the hemispherical cover plate.

[0008] Furthermore, it also includes a cooling fan located between the lead screw and the sample pressure plate; a bracket consisting of multiple L-shaped ribs arranged in an equidistant ring is provided below the lead screw, the bottom of the bracket is fixed to the top of the sample pressure plate, and the cooling fan is located below the bracket.

[0009] Furthermore, the top wall of the hemispherical cover plate has an air inlet, the side wall of the hemispherical cover plate has an air outlet, and the sample pressure plate has ventilation holes. The operation of the cooling fan causes air to enter the hemispherical cover plate from the top and pass through the ventilation holes to cool the centrifuge tube and be discharged from the air outlet.

[0010] Furthermore, the air inlet and air outlet are provided with a backstop plate a and a backstop plate b, respectively. The movement direction of the backstop plate a is towards the inside of the hemispherical cover plate, and the movement direction of the backstop plate b is towards the outside of the hemispherical cover plate.

[0011] Furthermore, a handle is provided above the lead screw.

[0012] The beneficial effects of the technical solution provided in this application are as follows:

[0013] This cross-contamination-preventing metal bath incorporates a sample clamping plate. Adjustment via a screw on a nut secures the plate, firmly holding the centrifuge tube cap in place and preventing the cap from popping open due to increased vapor pressure inside the tube. This prevents small DNA / RNA fragments from positive samples from splashing and forming aerosols, thus avoiding contamination of other negative samples. This significantly improves the accuracy and reliability of DNA / RNA testing, ensuring that experimental results accurately reflect the sample's condition and providing precise evidence for medical diagnosis. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the cross-contamination prevention metal bath of this utility model;

[0016] Figure 2 This is a half-sectional view of the rear side of the metal bath for preventing cross-contamination according to this utility model;

[0017] Figure 3 This is a top view of the cross-contamination prevention metal bath of this utility model.

[0018] In the diagram: 10 Metal bath body, 11 Control panel; 20 Heating template; 30 Hemispherical cover, 31 Air inlet, 32 Air outlet, 33 No-reverse plate a, 34 No-reverse plate b; 40 Centrifuge tube plug pressure assembly, 41 Lead screw, 42 Nut, 43 Sample pressure plate, 44 Cooling fan, 45 Bracket, 46 Handle. Detailed Implementation

[0019] The technical solution of this utility model will be clearly and completely described below with reference to its embodiments. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0020] Reference Figure 1-3 A cross-contamination-preventing metal bath includes a metal bath body 10 and a heating template 20 with upward-facing centrifuge tube openings disposed inside it. A hemispherical cover plate 30 is hinged above the metal bath body 10. A centrifuge tube plug pressure assembly 40 extends through the hemispherical cover plate 30 and extends above it. The centrifuge tube plug pressure assembly 40 includes a sample pressure plate 43 at its bottom for abutting against the centrifuge tube plug and a lead screw 41 disposed above the sample pressure plate 43. The lead screw 41 is threadedly connected to a nut 42 disposed on the hemispherical cover plate 30 and extends above the hemispherical cover plate 30. Force is applied to the lead screw 41 to feed it linearly and drive the sample pressure plate 43 to rise or fall. The lead screw 41 is threadedly connected to the nut 42, and the sample pressure plate 43 is located at the bottom of the lead screw 41 for abutting against the centrifuge tube plug. By rotating the lead screw 41, the sample pressure plate 43 can be raised or lowered, thereby applying or releasing pressure to the centrifuge tube plug. The key function of this design is to effectively prevent the centrifuge tube caps from popping open due to increased internal vapor pressure during high-temperature incubation. This avoids the splashing of DNA / RNA fragments from positive samples, preventing cross-contamination and ensuring the accuracy and reliability of experimental results. Simultaneously, this simple and easy-to-operate centrifuge tube cap pressure assembly 40 not only improves the convenience of experimental operations but also enhances the practicality and safety of the equipment, providing better protection for the laboratory environment and reducing the risk of aerosol contamination. Its working principle is based on the threaded transmission mechanism of the lead screw 41 and nut 42. When force is applied to the top of the lead screw 41, the lead screw 41 will perform a linear threaded feed motion within the nut 42. Since the lead screw 41 is connected to the sample pressure plate 43, the sample pressure plate 43 will move up and down as the lead screw 41 rotates. When pressure needs to be applied to the centrifuge tube stopper, rotating the lead screw 41 clockwise causes it to move downwards under the constraint of the nut 42, lowering the sample pressure plate 43 and pressing it against the centrifuge tube stopper. Conversely, rotating the lead screw 41 counterclockwise causes it to move upwards, raising the sample pressure plate 43 and releasing the pressure on the centrifuge tube stopper. This precise mechanical transmission design allows operators to easily control the position of the sample pressure plate 43, ensuring that appropriate pressure is always applied to the stopper during centrifuge tube incubation, effectively preventing the stopper from popping open and thus preventing cross-contamination.

[0021] In some embodiments, the sample pressure plate 43 is a circular plate with an arc-shaped slope at its outer edge that fits the inner wall of the hemispherical cover plate 30. The arc-shaped slope design allows the sample pressure plate 43 to smoothly fit against the inner wall of the hemispherical cover plate 30 during its ascent, avoiding wear caused by shape mismatch and extending the service life of the equipment.

[0022] In some embodiments, a cooling fan 44 is also included, located between the lead screw 41 and the sample pressure plate 43. Below the lead screw 41 is a bracket 45 composed of multiple L-shaped ribs arranged in an equidistant ring. The bottom of the bracket 45 is fixed to the top of the sample pressure plate 43, and the cooling fan 44 is positioned below the bracket 45. The main function of the cooling fan 44 is to rapidly reduce the thermal pressure inside the centrifuge tubes through air cooling after the metal bath stops heating. This helps prevent the centrifuge tube caps from popping open due to excessive internal pressure, avoiding sample leakage and cross-contamination. Simultaneously, the cooling fan 44 also accelerates the dissipation of heat inside the equipment, shortens the cooling time, and improves experimental efficiency.

[0023] In some embodiments, the hemispherical cover plate 30 has an air inlet 31 on its top wall and an air outlet 32 ​​on its side wall. The sample pressure plate 43 has ventilation holes. The operation of the cooling fan 44 allows air to enter the hemispherical cover plate 30 from the top, pass through the ventilation holes to cool the centrifuge tubes, and exit from the air outlet 32. The addition of air holes / outlets provides an airflow path. After the metal bath equipment completes the heating process and stops heating, the cooling fan 44 starts operating. External air enters the equipment from the air inlet 31 on the top wall of the hemispherical cover plate 30. The airflow generated by the cooling fan 44 acts directly on the surface of the centrifuge tubes through the ventilation holes on the sample pressure plate 43, accelerating the dissipation of heat inside the centrifuge tubes, thereby reducing the temperature and pressure inside the centrifuge tubes. This air-cooling method can quickly reduce the pressure inside the centrifuge tubes, preventing the centrifuge tube lid from popping open due to excessive pressure. Subsequently, the hot air is discharged from the air outlet 32 ​​on the side wall of the hemispherical cover plate 30, forming a complete air circulation path.

[0024] In some embodiments, the air inlet 31 and air outlet 32 ​​are equipped with anti-reverse plates a33 and b34, respectively. Anti-reverse plate a33 moves towards the inside of the hemispherical cover 30, while anti-reverse plate b34 moves towards the outside of the hemispherical cover 30. The main function of this design is to ensure that when the cooling fan 44 is operating, air can only enter the hemispherical cover 30 from the air inlet 31 and exit from the air outlet 32, forming a unidirectional airflow path. Anti-reverse plates a33 and b34 prevent backflow of air, avoiding the entry of pollutants from the outside air into the equipment, further reducing the risk of cross-contamination. Simultaneously, this unidirectional airflow design improves heat dissipation efficiency, ensuring that the heat and pressure inside the centrifuge tubes can be rapidly reduced, preventing the centrifuge tube caps from popping open due to excessive pressure, and ensuring the accuracy and safety of the experiment.

[0025] In some embodiments, a handle 46 is provided above the lead screw 41. The main function of the handle 46 is to provide the operator with a more convenient and labor-saving operating method, making it easier to apply force to the lead screw 41. Through the handle 46, the operator can more easily rotate the lead screw 41, thereby precisely controlling the up and down movement of the sample pressure plate 43, ensuring that the sample pressure plate 43 can accurately press or release the centrifuge tube plug.

[0026] In this cross-contamination-preventing metal bath, a temperature sensor and a buzzer can be further integrated and electrically connected to the control panel 11 on the metal bath body 10. Additionally, the cooling fan 44 is also electrically connected to the control panel 11. The main function of this design is to monitor the internal temperature of the equipment in real time during the experiment and automatically stop the cooling fan 44 when the temperature drops to a set safe value. Simultaneously, an alarm signal is emitted via a buzzer, prompting the operator that it is safe to open the incubator and remove the samples. The temperature sensor accurately detects temperature changes inside the metal bath and feeds the data back to the control panel. When the temperature drops to the set value, the control panel issues a command to stop the cooling fan 44 to avoid unnecessary energy consumption and equipment wear. At the same time, the buzzer's alarm clearly indicates to the operator that the equipment has cooled to a safe temperature, the pressure inside the centrifuge tubes has decreased, and the temperature of the heating element has dropped. Opening the incubator at this point will not cause the centrifuge tube caps to pop open due to pressure difference, nor will it cause injury to the operator due to high temperature. This function not only improves the safety of experimental operations but also effectively reduces the risk of aerosol contamination in the laboratory, ensuring the accuracy and reliability of experimental results.

[0027] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A cross-contamination-preventing metal bath, comprising a metal bath body (10) and a heating template (20) disposed therein with upward-facing centrifuge tube openings, wherein a hemispherical cover plate (30) is hinged above the metal bath body (10); characterized in that, The hemispherical cover plate (30) is provided with a centrifuge tube plug pressure assembly (40) extending through and above it. The centrifuge tube plug pressure assembly (40) includes a sample pressure plate (43) at the bottom for abutting the centrifuge tube plug and a lead screw (41) provided above the sample pressure plate (43). The lead screw (41) is threaded into a nut (42) provided on the hemispherical cover plate (30) and extends above the hemispherical cover plate (30). Force is applied to the lead screw (41) to make its straight thread feed and drive the sample pressure plate (43) to rise or fall.

2. The anti-cross-contamination metal bath as described in claim 1, characterized in that, The sample pressure plate (43) is a circular plate with an arc-shaped slope at its outer edge that fits the inner wall of the hemispherical cover plate (30).

3. The anti-cross-contamination metal bath as described in claim 1, characterized in that, It also includes a cooling fan (44) located between the lead screw (41) and the sample pressure plate (43); a bracket (45) composed of multiple L-shaped ribs arranged in annular equidistant arrangement is provided below the lead screw (41), the bottom of the bracket (45) is fixed to the top of the sample pressure plate (43), and the cooling fan (44) is located below the bracket (45).

4. The anti-cross-contamination metal bath as described in claim 3, characterized in that, The top wall of the hemispherical cover plate (30) is provided with an air inlet (31), the side wall of the hemispherical cover plate (30) is provided with an air outlet (32), the sample pressure plate (43) is provided with ventilation holes, and the operation of the cooling fan (44) causes air to enter the hemispherical cover plate (30) from the top and pass through the ventilation holes to cool the centrifuge tube and be discharged from the air outlet (32).

5. The anti-cross-contamination metal bath as described in claim 4, characterized in that, The air inlet (31) and air outlet (32) are provided with anti-reverse plate a (33) and anti-reverse plate b (34). The movement direction of anti-reverse plate a (33) is towards the inside of the hemispherical cover plate (30), and the movement direction of anti-reverse plate b (34) is towards the outside of the hemispherical cover plate (30).

6. The anti-cross-contamination metal bath as described in claim 1, characterized in that, A handle (46) is provided above the lead screw (41).

Images