Brain slice fixing device for killed medical experiment mice
By using a gear-driven and elastic block-designed fixation device, the problems of uneven clamping and flexible protection in mouse brain slice fixation devices were solved, achieving uniform fixation and tissue protection of the mouse brain, thus improving slice quality and experimental accuracy.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- KUNMING MEDICAL UNIVERSITY
- Filing Date
- 2025-05-27
- Publication Date
- 2026-05-12
AI Technical Summary
Existing mouse brain slice fixation devices are difficult to achieve uniform and stable clamping, resulting in tissue deformation and poor fixation effect. Furthermore, they lack flexible protection and are prone to damaging fragile nerve tissue.
The bevel gear transmission structure, consisting of a transmission cylinder, transmission gear, and receiving gear, combined with auxiliary limiting components and elastic block design, achieves synchronous limiting and flexible protection, adapts to the irregular shape of the mouse brain, and monitors the fixing force through a pressure sensor.
This method achieves uniform and stable fixation of the mouse brain, reduces tissue deformation, improves section quality and experimental accuracy, protects fragile nerve tissue, and ensures fixation effectiveness while avoiding damage.
Smart Images
Figure CN224231390U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of medical experiments, and more specifically, to a device for fixing brain slices after euthanizing medical experimental mice. Background Technology
[0002] In the field of medical research, analyzing slices of the brains of laboratory mice is an important means of exploring neural structures and pathological mechanisms.
[0003] However, existing mouse brain slice fixation devices struggle to achieve uniform and stable clamping of the mouse brain. The lack of synchronization between different clamping components easily leads to uneven stress on the brain during fixation, causing tissue deformation and affecting slice quality. Furthermore, due to the irregular shape of the mouse brain, a single fixation method cannot adapt to its complex morphology, resulting in poor fixation effects and even brain tissue slippage or displacement during slicing, reducing experimental accuracy and repeatability. In addition, existing devices mostly employ rigid fixation methods, lacking flexible protection for brain tissue and easily causing damage to fragile neural tissue. How to invent a brain slice fixation device for euthanized medical laboratory mice to improve these problems has become an urgent issue for those skilled in the art. Utility Model Content
[0004] To overcome the above shortcomings, this utility model provides a device for fixing brain slices after the sacrifice of medical experimental mice, which aims to improve the problem that existing mouse brain slice fixing devices are difficult to achieve uniform and stable clamping of mouse brains.
[0005] This invention is achieved as follows: a device for fixing brain slices after euthanasia of medical laboratory mice, comprising...
[0006] A placement platform; a brain limiting mechanism, the brain limiting mechanism including a synchronous limiting component disposed inside the placement platform, the synchronous limiting component including a transmission cylinder, the transmission cylinder being limited and installed through one end inside the placement platform, a transmission gear being fixedly installed at one end of the transmission cylinder inside the placement platform, receiving gears being meshed on both sides of the transmission gear, connecting cylinders being fixedly connected at one end of the receiving gears near both sides of the placement platform, a first screw being threadedly driven inside the connecting cylinder, and a transmission plate being driven externally to the first screw;
[0007] An auxiliary limiting component is connected above the synchronous limiting component, and the auxiliary limiting component is installed on the transmission plate.
[0008] In a preferred embodiment of this utility model, the synchronous limiting component further includes a transmission rod, which is fixedly connected to the end of the transmission gear away from the transmission cylinder. Multiple extension gears are equally spaced around the outside of the transmission rod, and receiving gears are respectively meshed and driven on both sides of the extension gears. Connecting cylinders are respectively fixedly connected to the ends of the receiving gears near the two sides of the placement platform.
[0009] In a preferred embodiment of this utility model, the ends of the connecting cylinder are respectively limited to rotate through both sides of the placement platform, and the transmission gear, receiving gear, and receiving gear are all bevel gears.
[0010] In a preferred embodiment of this utility model, one end of the first screw is threadedly inserted into the interior of the connecting cylinder, and a limiting rod is slidably inserted into the outer side of the other end face of the first screw. Multiple sets of the limiting rods are evenly distributed around the screw, and the other ends of the multiple sets of limiting rods are fixedly connected to the inner side of the transmission plate.
[0011] In a preferred embodiment of this utility model, the auxiliary limiting component includes a second threaded rod, which is threadedly driven inside the first screw and threaded through the transmission plate. A base plate is fixedly connected to the bottom of the transmission plate, and the base plate is slidably embedded in the bottom of the placement platform.
[0012] In a preferred embodiment of this utility model, a handle is fixedly connected to the outer end of the second threaded rod, and the handle is located on the outside of the transmission plate.
[0013] In a preferred embodiment of this utility model, a limiting shell is fixedly connected to the top of the transmission plate, and an elastic block is slidably provided at the middle of one end of the limiting shell near the placement platform. A push plate is fixedly connected to the outer end of the elastic block, and the push plate makes limiting contact with the mouse brain.
[0014] In a preferred embodiment of this utility model, protrusions are respectively provided on both sides of one end of the elastic block located inside the limiting shell. The elastic block cooperates with the protrusions to limit sliding within the limiting shell, and a spring is provided between the elastic block and the inside of the limiting shell.
[0015] In a preferred embodiment of this utility model, a silicone sleeve is fitted onto the outside of the push plate.
[0016] In a preferred embodiment of this invention, a pressure sensor is provided inside the elastic block, and the pressure sensor abuts against the spring.
[0017] The beneficial effects of this utility model are as follows: The brain slice fixation device obtained by the above design of this utility model for euthanized medical experimental mice has synchronous and precise positioning during use: through the bevel gear transmission structure of transmission cylinder, transmission gear and receiving gear, the synchronous driving of the push plates on both sides is realized, which can uniformly limit and fix the mouse brain, avoid tissue deformation caused by asynchronous clamping in traditional devices, and ensure the integrity and quality of the slice.
[0018] Adaptive and flexible adjustment: To accommodate the irregular shape of the mouse brain, the second threaded rod can be rotated by adjusting the handle individually, which enables independent control of a single push plate. This allows for precise adaptation to mouse brains of different shapes, improving the versatility and practicality of the fixation device.
[0019] Flexible tissue protection: The push plate is fitted with a silicone sleeve, and a spring is set between the elastic block and the limiting shell. This flexible structure design can effectively buffer external forces when fixing the mouse brain, reduce damage to fragile nerve tissue, and improve the effectiveness of experimental samples.
[0020] Real-time pressure monitoring: The pressure sensor inside the elastic block can monitor the pressure applied to the mouse brain in real time during the fixation process, which allows the experimenter to adjust the fixation force according to the pressure data, avoiding the problem of tissue damage due to excessive pressure or insecure fixation due to insufficient pressure. Attached Figure Description
[0021] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.
[0022] Figure 1 This is a schematic diagram of one side of the structure provided by an embodiment of the present invention;
[0023] Figure 2 A schematic diagram of the internal structure provided for an embodiment of this utility model;
[0024] Figure 3 A schematic diagram of the synchronous limiting component structure provided for an embodiment of this utility model;
[0025] Figure 4 A schematic diagram of the auxiliary limiting component structure provided for an embodiment of this utility model.
[0026] In the diagram: 100 - Placement platform; 200 - Brain limiting mechanism; 210 - Synchronous limiting component; 211 - Transmission cylinder; 212 - Transmission gear; 213 - Transmission rod; 214 - Extension gear; 215 - Receiving gear; 216 - Connecting cylinder; 217 - First screw; 218 - Transmission plate; 219 - Limiting rod; 220 - Auxiliary limiting component; 221 - Second threaded rod; 222 - Handle; 223 - Base plate; 224 - Push plate; 225 - Elastic block; 226 - Spring; 227 - Limiting shell. Detailed Implementation
[0027] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.
[0028] Please see Figure 1 and Figure 2 This utility model provides a technical solution: a device for fixing brain slices after euthanasia of medical laboratory mice, comprising...
[0029] Placement platform 100; brain limiting mechanism 200, the brain limiting mechanism 200 includes a synchronous limiting component 210, the synchronous limiting component 210 is disposed inside the placement platform 100, the synchronous limiting component 210 includes a transmission cylinder 211, the transmission cylinder 211 is limited and installed through one end inside the placement platform 100, the end of the transmission cylinder 211 inside the placement platform 100 is fixedly installed with a transmission gear 212, the two sides of the transmission gear 212 are respectively meshed with receiving gears 215, the ends of the receiving gears 215 near the two sides of the placement platform 100 are respectively fixed A connecting cylinder 216 is connected, and a first screw 217 is installed inside the connecting cylinder 216. A transmission plate 218 is connected to the outside of the first screw 217. An auxiliary limiting component 220 is connected above the synchronous limiting component 210 and is installed on the transmission plate 218. For the irregular shape of the mouse brain, the second threaded rod 221 can be rotated by adjusting the handle 222, which can realize the independent control of a single push plate 224, thereby accurately adapting to mouse brains of different shapes and improving the versatility and practicality of the fixing device.
[0030] Please see Figure 3 and Figure 4The synchronous limiting assembly 210 also includes a transmission rod 213, which is fixedly connected to the end of the transmission gear 212 away from the transmission cylinder 211. Multiple extension gears 214 are evenly sleeved on the outside of the transmission rod 213. Receiving gears 215 are meshed on both sides of each extension gear 214. Connecting cylinders 216 are fixedly connected to the ends of the receiving gears 215 near both sides of the placement platform 100. The ends of the connecting cylinders 216 are respectively limited to rotate through both sides of the placement platform 100. The transmission gear 212, receiving gear 215, and receiving gear 216 are all bevel gears, which facilitate transmission.
[0031] One end of the first screw 217 is threaded into the interior of the connecting cylinder 216. A limit rod 219 is slidably inserted into the outer side of the other end face of the first screw 217. Multiple sets of limit rods 219 are evenly spaced around the screw, and the other ends of these limit rods 219 are fixedly connected to the inner side of the transmission plate 218. To ensure that the first screw 217 moves axially when the connecting cylinder 216 rotates, the two should be connected by a one-way thread. Since the connecting cylinders 216 on both sides rotate synchronously via bevel gear transmission, to allow both first screws 217 to move inwards to drive the push plate 224 closer to the mouse brain, the thread directions of the connecting cylinders 216 and the first screw 217 on both sides should be opposite. That is, the left connecting cylinder 216 and the first screw 217 use right-hand threads, while the right side uses left-hand threads. Thus, when the connecting cylinders 216 rotate synchronously, both first screws 217 can simultaneously translate inwards.
[0032] The auxiliary limiting component 220 includes a second threaded rod 221. The second threaded rod 221 is threadedly driven inside the first screw 217. The second threaded rod 221 threadedly passes through a transmission plate 218. A base plate 223 is fixedly connected to the bottom of the transmission plate 218. The base plate 223 is slidably embedded in the bottom of the placement platform 100. A handle 222 is fixedly connected to the outer end of the second threaded rod 221. The handle 222 is located outside the transmission plate 218. To accommodate the irregular shape of the mouse brain, the handle 222 can be individually adjusted to rotate the second threaded rod 221, enabling independent control of a single push plate 224. This allows for precise adaptation to mouse brains of different shapes, improving the versatility and practicality of the fixing device.
[0033] A limiting shell 227 is fixedly connected to the top of the transmission plate 218. An elastic block 225 is slidably positioned at the middle of one end of the limiting shell 227 near the placement platform 100. A push plate 224 is fixedly connected to the outer end of the elastic block 225, and the push plate 224 engages with the mouse brain for limiting contact. Protrusions are provided on both sides of one end of the elastic block 225 inside the limiting shell 227. The elastic block 225 slides within the limiting shell 227 in conjunction with the protrusions. A spring 226 is positioned between the elastic block 225 and the inside of the limiting shell 227. A silicone sleeve is fitted over the push plate 224. This flexible structure design effectively buffers external forces when fixing the mouse brain, reducing damage to fragile nerve tissue and improving the validity of the experimental sample.
[0034] The elastic block 225 is equipped with a pressure sensor, which abuts against the spring 226. The pressure sensor can be electrically connected to an alarm light or other alarm device to avoid excessive compression of the mouse brain, which would affect the slice's quality.
[0035] Working Principle: Synchronous Limiting Operation: Rotating the transmission cylinder 211 drives the transmission gear 212 to rotate. Since the transmission gear 212 meshes with the receiving gears 215 on both sides, and both are bevel gears, according to the bevel gear transmission principle, the rotation of the transmission gear 212 will synchronously drive the receiving gears 215 on both sides to rotate. When the receiving gears 215 rotate, they drive the connecting cylinder 216 to rotate. The first screw 217 inside the connecting cylinder 216 is threaded with the connecting cylinder 216. While the connecting cylinder 216 rotates, the first screw 217 moves axially, thereby driving the transmission plate 218 to move, ultimately causing the push plates 224 on both sides to move closer or further apart synchronously, which is used to initially limit the mouse brain placed on the placement platform 100.
[0036] Shape adaptation adjustment: When encountering an irregularly shaped mouse brain, if there is a problem with the fixation after initial positioning, the handle 222 can be rotated. The handle 222 drives the second threaded rod 221 to rotate inside the first screw 217. Since the thread of the second threaded rod 221 passes through the transmission plate 218, the rotation of the second threaded rod 221 will cause the transmission plate 218 to move along the axial direction of the second threaded rod 221, thereby achieving precise adjustment of the position of the single push plate 224, so that the push plate 224 can fit the irregular surface of the mouse brain and complete the precise fixation.
[0037] Flexible protection and pressure monitoring: When the push plate 224 contacts the mouse brain for fixation, the push plate 224 receives a reaction force, which is transmitted to the spring 226 through the elastic block 225. The spring 226 undergoes elastic deformation, acting as a buffer to prevent rigid compression damage to brain tissue. Simultaneously, the pressure sensor inside the elastic block 225 monitors the pressure on the spring 226 in real time and feeds the pressure data back to the experimenters. The experimenters can adjust the fixation force based on the data to ensure effective fixation while protecting brain tissue.
[0038] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.
Claims
1. A device for fixing brain slices after euthanizing a medical laboratory mouse, characterized in that, include Placement platform; A brain limiting mechanism includes a synchronous limiting component disposed inside the placement platform. The synchronous limiting component includes a transmission cylinder, which is limited and installed inside one end of the placement platform. A transmission gear is fixedly installed at one end of the transmission cylinder inside the placement platform. Receiving gears are respectively meshed on both sides of the transmission gear. Connecting cylinders are respectively fixedly connected to one end of the receiving gears near the two sides of the placement platform. A first screw is threaded inside the connecting cylinder, and a transmission plate is externally connected to the first screw. An auxiliary limiting component is connected above the synchronous limiting component, and the auxiliary limiting component is installed on the transmission plate.
2. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 1, characterized in that: The synchronous limiting component also includes a transmission rod, which is fixedly connected to the end of the transmission gear away from the transmission cylinder. Multiple extension gears are equally spaced around the outside of the transmission rod. Receiving gears are respectively meshed and driven on both sides of the extension gears. Connecting cylinders are respectively fixedly connected to the ends of the receiving gears near the two sides of the placement platform.
3. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 2, characterized in that: The ends of the connecting cylinder are respectively limited to rotate through both sides of the placement platform, and the transmission gear, receiving gear and receiving gear are all bevel gears.
4. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 2, characterized in that: One end of the first screw is threaded into the inside of the connecting cylinder, and a limit rod is slidably inserted into the outer side of the other end face of the first screw. Multiple sets of the limit rods are evenly distributed around the screw, and the other ends of the multiple sets of limit rods are fixedly connected to the inner side of the transmission plate.
5. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 4, characterized in that: The auxiliary limiting component includes a second threaded rod, which is threadedly driven inside the first screw. The second threaded rod is threaded through the transmission plate. A base plate is fixedly connected to the bottom of the transmission plate, and the base plate is slidably embedded in the bottom of the placement platform.
6. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 5, characterized in that: A handle is fixedly connected to the outer end of the second threaded rod, and the handle is located on the outside of the transmission plate.
7. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 5, characterized in that: The top of the transmission plate is fixedly connected to a limiting shell. An elastic block is slidably provided at the middle of one end of the limiting shell near the placement platform. A push plate is fixedly connected to the outer end of the elastic block. The push plate makes limiting contact with the mouse brain.
8. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 7, characterized in that: The elastic block is provided with protrusions on both sides of one end inside the limiting shell. The elastic block slides within the limiting shell in conjunction with the protrusions. A spring is provided between the elastic block and the inside of the limiting shell.
9. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 8, characterized in that: The push plate is fitted with a silicone sleeve.
10. The device for fixing brain slices after euthanasia of a medical laboratory mouse as described in claim 8, characterized in that: A pressure sensor is installed inside the elastic block, and the pressure sensor abuts against the spring.