Annular fluorescence excitation loop for transduction and synergy

Abstract

The utility model discloses an annular fluorescence excitation loop for transduction and synergy. The annular fluorescence excitation loop comprises a slide, a dichroscope, a first reflecting mirror, a second reflecting mirror and a third reflecting mirror which are sequentially arranged to form a closed-loop light path, the dichroscope is arranged on the laser and fluorescence signal emitting side of the slide, and the dichroscope is used for reflecting the incident laser to the first reflector and transmitting the fluorescence signal from the slide; the first reflector is used for receiving the residual laser reflected by the dichroscope and reflecting the residual laser to the second reflector; the second reflecting mirror is used for receiving the residual laser reflected by the first reflecting mirror and reflecting the residual laser to the third reflecting mirror; the third reflector is used for receiving the residual laser reflected by the second reflector and reflecting the residual laser to the slide; after the scheme is adopted, the laser utilization rate can be improved, so that the problem of low laser utilization rate in the prior art is practically solved.

Description

Technical Field

[0001] This utility model relates to the technical field of fluorescence excitation circuits, and in particular to a ring-shaped fluorescence excitation circuit for energy conversion enhancement. Background Technology

[0002] Traditional fluorescence excitation systems often employ a single optical path design, resulting in low laser utilization. The incident laser penetrates the sample slide only once, and the residual laser that does not excite fluorescence is directly lost, leading to resource waste. Furthermore, signal separation relies on filters, requiring multiple sets of bandpass filters to distinguish between laser and fluorescence signals, which leads to light energy attenuation and increases system complexity and maintenance costs. In addition, background noise is significant, and stray laser light remaining on the filters easily forms background interference, affecting detection sensitivity. Utility Model Content

[0003] The purpose of this invention is to provide a ring-shaped fluorescence excitation circuit for improving transduction efficiency, so as to solve the problem of low laser utilization in the prior art.

[0004] To address the aforementioned technical problems, this invention provides a ring-shaped fluorescence excitation circuit for enhancing transduction efficiency, comprising a glass slide, a dichroic mirror, a first reflecting mirror, a second reflecting mirror, and a third reflecting mirror arranged sequentially to form a closed-loop optical path. The dichroic mirror is disposed on the laser and fluorescence signal emission side of the glass slide, and is used to reflect the incident laser to the first reflecting mirror and transmit the fluorescence signal from the glass slide. The first reflecting mirror is used to receive the residual laser reflected by the dichroic mirror and reflect it to the second reflecting mirror. The second reflecting mirror is used to receive the residual laser reflected by the first reflecting mirror and reflect it to the third reflecting mirror. The third reflecting mirror is used to receive the residual laser reflected by the second reflecting mirror and reflect it to the glass slide.

[0005] In one embodiment, the glass slide is made of quartz or optical glass, and the surface of the glass slide is coated with a fluorescent material layer.

[0006] In one embodiment, the surface of the dichroic mirror is at a 45° angle to the laser incident direction.

[0007] In one embodiment, the dichroic mirror covers the laser band (900-1100nm) and the fluorescence signal band (400-800nm).

[0008] In one embodiment, the dichroic mirror has a transmittance of ≥90% for fluorescence wavelength and a reflectance of ≥90% for laser wavelength.

[0009] In one embodiment, the mirror surface of the first reflector is at a 135° angle to the laser incident direction.

[0010] In one embodiment, the mirror surface of the second reflector is at a 45° angle to the laser incident direction.

[0011] In one embodiment, the surface of the third reflector is at a 135° angle to the laser incident direction.

[0012] The beneficial effects of this utility model are as follows:

[0013] 1. Significantly improves laser utilization: Residual laser light is recovered and re-excited through a closed-loop optical path;

[0014] 2. Significantly reduces hardware costs and complexity: Eliminates the need for multiple bandpass filters, reduces light energy loss, and reduces the number of system components;

[0015] 3. Optimize fluorescence signal-to-noise ratio: Increase fluorescence signal intensity, reduce background noise, and improve detection sensitivity;

[0016] 4. Enhanced compatibility and stability: The modular design is compatible with equipment such as fluorescence microscopes and flow cytometers, improving optical path calibration tolerance and reducing maintenance difficulty. Attached Figure Description

[0017] To more clearly illustrate the technical solution of this utility model, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0018] Figure 1 This is a structural schematic diagram provided by an embodiment of the present utility model.

[0019] The attached figures are labeled as follows:

[0020] 1. Glass slide; 2. Dichroic mirror; 3. First reflecting mirror; 4. Second reflecting mirror; 5. Third reflecting mirror. Detailed Implementation

[0021] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments.

[0022] This invention provides a ring-shaped fluorescence excitation circuit for enhancing transducer efficiency. This ring-shaped fluorescence excitation circuit is suitable for fluorescence microscopes, flow cytometers, or high-throughput fluorescence detection equipment. Its implementation is as follows: Figure 1As shown, the optical path includes a glass slide 1, a dichroic mirror 2, a first reflecting mirror 3, a second reflecting mirror 4, and a third reflecting mirror 5 arranged in sequence to form a closed-loop optical path. The dichroic mirror 2 is located on the laser and fluorescence signal emission side of the glass slide 1. The dichroic mirror 2 is used to reflect the incident laser to the first reflecting mirror 3 and transmit the fluorescence signal from the glass slide 1. The first reflecting mirror 3 is used to receive the residual laser reflected by the dichroic mirror 2 and reflect it to the second reflecting mirror 4. The second reflecting mirror 4 is used to receive the residual laser reflected by the first reflecting mirror 3 and reflect it to the third reflecting mirror 5. The third reflecting mirror 5 is used to receive the residual laser reflected by the second reflecting mirror 4 and reflect it back to the glass slide 1.

[0023] In application, the fluorescence and laser emitted from the glass slide 1 will be directed to the dichroic mirror 2. The emitted fluorescence will pass through the dichroic mirror 2 and be emitted out, while the emitted laser will be reflected by the dichroic mirror 2 to the first reflecting mirror 3. The first reflecting mirror 3 will then reflect the residual laser to the second reflecting mirror 4, and the second reflecting mirror 4 will then reflect the residual laser to the third reflecting mirror 5. Finally, the third reflecting mirror 5 will reflect the residual laser back to the glass slide 1, thereby realizing the reuse of the residual laser and effectively solving the problem of low laser utilization in existing technologies.

[0024] Preferably, in this embodiment, the glass slide 1 is made of quartz or optical glass, and the surface of the glass slide 1 is coated with a fluorescent material layer.

[0025] like Figure 1 As shown, in this embodiment, the surface of the dichroic mirror 2 is set at a 45° angle to the laser incident direction.

[0026] Preferably, in this embodiment, the dichroic mirror 2 is configured to cover the laser band (900-1100nm) and the fluorescence signal band (400-800nm).

[0027] Preferably, in this embodiment, the transmittance of the dichroic mirror 2 is ≥90% for fluorescence wavelength and the reflectance of the dichroic mirror 2 is ≥90% for laser wavelength.

[0028] like Figure 1 As shown, in this embodiment, the mirror surface of the first reflector 3 is set at a 135° angle to the laser incident direction.

[0029] After adopting the above configuration, the central axis of the first reflector 3 and the central axis of the dichroic mirror 2 share the same vertical axis, and the mirror surface forms a 135° angle with the horizontal plane, which is used to reflect the laser from the dichroic mirror 2 to the horizontal direction.

[0030] like Figure 1 As shown, in this embodiment, the mirror surface of the second reflector 4 is set at a 45° angle to the laser incident direction.

[0031] After adopting the above configuration, the central axis of the second reflector 4 is collinear with the optical path axis of the laser reflected by the first reflector 3, and the mirror surface is at a 45° angle to the horizontal plane, which is used to reflect the horizontally incident laser to the vertical upward direction.

[0032] like Figure 1 As shown, in this embodiment, the mirror surface of the third reflector 5 is set at a 135° angle to the laser incident direction.

[0033] After adopting the above configuration, the central axis of the third reflector 5 and the optical path axis of the laser reflected by the second reflector 4 are on the same vertical axis, and the mirror surface forms a 135° angle with the horizontal plane, which is used to reflect the vertically upward laser back to the incident surface of the glass slide 1.

[0034] The above description is the preferred embodiment of this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of this utility model, and these improvements and modifications are also considered to be within the protection scope of this utility model.

Claims

1. A ring-shaped fluorescence excitation circuit for enhancing transducer efficiency, characterized in that, It includes a glass slide, a dichroic mirror, a first reflecting mirror, a second reflecting mirror, and a third reflecting mirror arranged in sequence to form a closed-loop optical path; The dichroic mirror is disposed on the laser and fluorescence signal emitting side of the glass slide. The dichroic mirror is used to reflect the incident laser to the first reflecting mirror and transmit the fluorescence signal from the glass slide. The first reflector is used to receive the residual laser light reflected by the dichroic mirror and reflect it to the second reflector; The second reflector is used to receive the residual laser light reflected by the first reflector and reflect it to the third reflector; The third reflector is used to receive the residual laser light reflected by the second reflector and reflect it back onto the glass slide.

2. The ring-shaped fluorescence excitation circuit according to claim 1, characterized in that, The glass slide is made of quartz or optical glass, and the surface of the glass slide is coated with a fluorescent material layer.

3. The ring-shaped fluorescence excitation circuit according to claim 1, characterized in that, The surface of the dichroic mirror is at a 45° angle to the laser incident direction.

4. The ring-shaped fluorescence excitation circuit according to claim 1, characterized in that, The dichroic mirror covers both the laser band and the fluorescence signal band. The laser wavelength is 900-1100nm; The fluorescence signal wavelength is 400-800nm.

5. The ring-shaped fluorescence excitation circuit according to claim 1, characterized in that, The transmittance of the dichroic mirror is ≥90% for fluorescence wavelength, and the reflectance of the dichroic mirror is ≥90% for laser wavelength.

6. The ring-shaped fluorescence excitation circuit according to claim 1, characterized in that, The mirror surface of the first reflector forms a 135° angle with the laser incident direction.

7. The ring-shaped fluorescence excitation circuit according to claim 1, characterized in that, The mirror surface of the second reflector is at a 45° angle to the laser incident direction.

8. The ring-shaped fluorescence excitation circuit according to claim 1, characterized in that, The surface of the third reflecting mirror is at a 135° angle to the laser incident direction.

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