Portable preservation box for stem cell culture

Abstract

The utility model discloses a portable preservation box for stem cell culture, which comprises a box body, the inside of a mounting shell is divided into circulation channels through a plurality of groups of placing boxes, and the circulation channels are used for prolonging the forced contact time of air and dry ice; the air inlet end of the air pump communicates with two sets of extraction pipes used for extracting air to flow in the circulation channel. Through the design of the mounting shell, the placement box, the air pump, the extraction pipe, the exhaust pipe and the like, and through the cooperation of the air pump, the extraction pipe and the exhaust pipe, air in the box body can quickly flow in the mounting shell, and the air is forced to be in contact with dry ice in the placement box when flowing in the mounting shell, so that the air is quickly cooled and uniformly circulated; therefore, a cold source can be utilized to the maximum extent, energy waste is reduced, a set low-temperature environment can be recovered in a short time through an active refrigeration mode, the time for exposing stem cells to non-ideal temperature is shortened, and cell activity decline or cell death is avoided.

Description

Technical Field

[0001] This utility model relates to the field of stem cell preservation technology, specifically a portable stem cell culture preservation box. Background Technology

[0002] Portable stem cell culture storage kits are important devices in the biomedical field, primarily used for the short-term storage of stem cells to ensure their viability and stability in vitro. Stem cells are extremely sensitive to temperature and typically require preservation in strictly cryogenic environments to maintain their proliferation and differentiation potential.

[0003] Existing portable preservation boxes mostly use semiconductor refrigeration, dry ice, or ice packs to maintain low temperatures and employ sealed structures to minimize cold air loss. However, in practical use, frequent opening and closing of the preservation box to access test tubes leads to significant cold air leakage and rapid intrusion of external hot air, causing significant internal temperature fluctuations. Once the cold air inside the box is lost, relying solely on the existing refrigeration system is insufficient to restore the set temperature in a short time, exposing stem cells to an undesirable environment that may result in decreased cell activity, metabolic abnormalities, or even death. Therefore, we need to propose a portable preservation box for stem cell culture. Summary of the Invention

[0004] The purpose of this invention is to provide a portable stem cell culture preservation box that forces air into contact with a cold source, allowing the air to cool rapidly and circulate evenly. The temperature inside the preservation box can be restored to the set low temperature environment in a short time, reducing the time that stem cells are exposed to non-ideal temperatures, thereby avoiding cell activity decline or death, and solving the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A portable stem cell culture preservation box includes a box body, on both inner walls of the box body, mounting shells are installed, and multiple sets of inclined placement boxes are fixedly installed inside the mounting shells. Multiple sets of placement boxes are provided in the multiple sets of placement boxes for placing dry ice. The interior of the mounting shell is separated by multiple sets of placement boxes to extend the forced contact time between air and dry ice.

[0007] An air pump is fixedly installed at the bottom of the housing. The air pump's inlet end is connected to two sets of extraction pipes for drawing air to flow in the circulation channel. The extraction pipes are connected to the mounting shell. The air pump's outlet end is connected to an exhaust pipe for discharging air.

[0008] Preferably, a second support plate is fixedly installed on both inner walls of the box body, the bottom of the mounting shell is placed on the top of the second support plate, a mounting seat is fixedly connected to both sides of the mounting shell, and multiple sets of fixing blocks corresponding to the mounting seats are fixedly installed on both inner walls of the box body, and the mounting seats are fixedly installed in the fixing blocks by fixing screws.

[0009] Preferably, the bottom of the mounting housing is connected to a connecting pipe, the connecting pipe is inserted into the second support plate, the lower end of the connecting pipe is inserted into the inside of the extraction tube, and a sealing gasket is provided at the bottom of the connecting pipe, the sealing gasket is pressed against the upper end of the extraction tube.

[0010] Preferably, a fixing plate is fixedly installed on the top of the mounting shell, a protective net is provided inside the fixing plate, and an interception net plate for intercepting dry ice when it falls is fixedly installed inside the mounting shell.

[0011] Preferably, a first support plate is fixedly installed on the inner wall of the box, a test tube placement foam seat for placing test tubes is fixedly installed on the top of the first support plate, and a positioning frame for positioning test tubes is fixedly installed on the inner wall of the box, with multiple sets of insertion holes for inserting test tubes on the positioning frame.

[0012] Preferably, the end of the exhaust pipe extends between the first support plate and the positioning frame, and the positioning frame has multiple sets of flow holes for airflow.

[0013] Preferably, it also includes a sealing cover fixedly installed on the top of the box, the bottom of the sealing cover is provided with a sealing gasket, the top of the box is provided with a sealing groove that matches the sealing groove, and the sealing gasket is pressed into the sealing groove.

[0014] Preferably, limit switches are fixedly installed on both inner walls of the box, the sealing cover presses on the contacts of the limit switches, a temperature sensor for detecting the internal temperature of the box is fixedly installed at the bottom of the sealing cover, a controller is fixedly installed at the bottom of the box, and the air pump, temperature sensor and limit switches are all electrically connected to the controller.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] This invention, through the design of a mounting shell, a placement box, an air pump, an extraction pipe, and an exhaust pipe, enables rapid airflow within the mounting shell. This forced airflow through the mounting shell contacts the dry ice in the placement box, resulting in rapid cooling and uniform circulation. This maximizes the utilization of the cold source, reduces energy waste, and, through active cooling, quickly restores the set low-temperature environment, minimizing the time stem cells are exposed to non-ideal temperatures, thus preventing cell activity decline or death. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model;

[0018] Figure 2 This is a partial cross-sectional structural diagram of the present invention;

[0019] Figure 3 This is a schematic diagram of the structure of the box body of this utility model;

[0020] Figure 4 This is one of the structural diagrams of the test tube placement foam seat, positioning frame and mounting shell of this utility model;

[0021] Figure 5 This is the second schematic diagram of the structure of the test tube placement foam seat, positioning frame and mounting shell of this utility model;

[0022] Figure 6 This is a schematic diagram of the internal structure of the mounting shell of this utility model.

[0023] In the diagram: 1. Box body; 2. First support plate; 3. Test tube placement foam seat; 4. Positioning frame; 5. Second support plate; 6. Mounting shell; 7. Fixing block; 8. Mounting seat; 9. Fixing plate; 10. Protective net; 11. Air pump; 12. Extraction tube; 13. Placement box; 14. Placement slot; 15. Sealing cap; 16. Flow hole; 17. Temperature sensor; 18. Controller; 19. Limit switch; 20. Sealing gasket; 21. Sealing groove; 22. Insertion hole; 23. Interceptor plate; 24. Exhaust pipe; 25. Connecting pipe. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] Please see Figure 1-6 This utility model provides a technical solution:

[0026] A portable stem cell culture preservation box includes a box body 1. The inner walls of both sides of the box body 1 are equipped with mounting shells 6. Multiple sets of inclined placement boxes 13 are fixedly installed inside the mounting shells 6. Multiple sets of placement slots 14 for placing dry ice are opened in the multiple sets of placement boxes 13. The interior of the mounting shell 6 is separated by multiple sets of placement boxes 13 to extend the forced contact time between air and dry ice.

[0027] An air pump 11 is fixedly installed at the bottom of the housing 1. The air inlet of the air pump 11 is connected to two sets of extraction pipes 12 for drawing air to flow in the circulation channel. The extraction pipes 12 are connected to the mounting shell 6. The air outlet of the air pump 11 is connected to an exhaust pipe 24 for air discharge.

[0028] It should be noted that dry ice is placed in the placement tank 14 as a cold source. When air flows in the circulation channel, it can prolong the forced contact time between the air and the dry ice, thereby improving the cooling effect on the air.

[0029] In an optional embodiment: a second support plate 5 is fixedly installed on both inner walls of the housing 1, the bottom of the mounting shell 6 is placed on the top of the second support plate 5, mounting bases 8 are fixedly connected to both sides of the mounting shell 6, and multiple sets of fixing blocks 7 corresponding to the mounting bases 8 are fixedly installed on both inner walls of the housing 1, and the mounting bases 8 are fixedly installed in the fixing blocks 7 by fixing screws.

[0030] It should be noted that by using the mounting base 8 in conjunction with the fixing block 7, dry ice can be added into the placement box 13 after the mounting shell 6 is removed.

[0031] In an optional embodiment: the bottom of the mounting housing 6 is connected to a connecting pipe 25, the connecting pipe 25 is inserted into the second support plate 5, the lower end of the connecting pipe 25 is inserted into the inside of the extraction tube 12, and a sealing gasket is provided at the bottom of the connecting pipe 25, the sealing gasket is pressed against the upper end of the extraction tube 12.

[0032] It should be noted that the connection pipe 25 allows the mounting housing 6 and the extraction pipe 12 to be separated. The sealing gasket ensures a good seal between the connection pipe 25 and the extraction pipe 12 after the mounting housing 6 is installed.

[0033] In an optional embodiment: a fixing plate 9 is fixedly installed on the top of the mounting shell 6, a protective net 10 is provided inside the fixing plate 9, and an interception net plate 23 for intercepting dry ice when it falls is fixedly installed inside the mounting shell 6.

[0034] It should be noted that the protective net 10 can prevent foreign objects from falling into the interior of the mounting shell 6, and can also prevent dry ice from falling from the mounting shell 6 into the interior of the box 1 when the storage box is bumped during transportation. The intercepting mesh plate 23 can prevent dry ice from clogging the connecting pipe 25.

[0035] In an optional embodiment: a first support plate 2 is fixedly installed on the inner wall of the box 1, a test tube placement foam seat 3 for placing test tubes is fixedly installed on the top of the first support plate 2, and a positioning frame 4 for positioning test tubes is fixedly installed on the inner wall of the box 1. The positioning frame 4 has multiple sets of insertion holes 22 for inserting test tubes.

[0036] It should be noted that the positioning frame 4 serves to block the air inside the box 1, which can reduce the leakage of cold air when taking it out or placing it. The test tube can be inserted into the test tube placement foam seat 3 through the insertion hole 22.

[0037] In an optional embodiment, the end of the exhaust pipe 24 extends between the first support plate 2 and the positioning frame 4, and the positioning frame 4 has a plurality of flow holes 16 for airflow.

[0038] It should be noted that the air inside the housing 1 can flow through the flow hole 16.

[0039] In an optional embodiment, a sealing cover 15 is also included, which is fixedly installed on the top of the housing 1. A sealing gasket 20 is provided at the bottom of the sealing cover 15. A sealing groove 21 that is adapted to the sealing groove 21 is opened on the top of the housing 1. The sealing gasket 20 is pressed into the sealing groove 21.

[0040] It should be noted that the sealing gasket 20, together with the sealing groove 21, is used to seal the space between the box body 1 and the sealing cover 15, reducing the leakage of cold air. In addition, a telescopic handle is installed on the top of the sealing cover 15, which can be used to move and carry the storage box.

[0041] In an optional embodiment: limit switches 19 are fixedly installed on both inner walls of the housing 1, and a sealing cover 15 presses on the contacts of the limit switches 19. A temperature sensor 17 for detecting the internal temperature of the housing 1 is fixedly installed at the bottom of the sealing cover 15. A controller 18 is fixedly installed at the bottom of the housing 1. The air pump 11, the temperature sensor 17 and the limit switches 19 are all electrically connected to the controller 18.

[0042] It should be noted that by linking the limit switch 19, temperature sensor 17, air pump 11 and controller 18, the internal temperature of the chamber 1 can be automatically cooled after the test tube is picked up or placed, so that the internal temperature of the chamber 1 remains stable, creating a better preservation environment for the stem cells inside the chamber 1, so as to facilitate the short-term storage of stem cells. A power supply for powering the limit switch 19, temperature sensor 17, air pump 11 and controller 18 can be installed inside the chamber 1.

[0043] The usage process of this utility model is as follows: When it is necessary to place the test tube, open the sealing cap 15, reset the contacts of the limit switch 19, insert the test tube into the test tube placement foam seat 3 through the insertion hole 22, and fix the test tube through the test tube placement foam seat 3 and the positioning frame 4.

[0044] After placing the test tube, close the sealing cap 15. When the sealing cap 15 is closed, it presses the contact point, triggering a signal. Upon receiving the trigger signal, the controller 18 starts the air pump 11. The air pump 11 draws air into the mounting shell 6 through the extraction pipe 12. The air enters the mounting shell 6 after passing through the protective net 10. As the air flows through the circulation channel, it is forced to come into contact with dry ice for rapid cooling. After cooling, the air is discharged into the interior of the chamber 1 through the extraction pipe 12 to cool the interior of the chamber 1. The air inside the chamber 1 can pass through the circulation hole 16 and re-enter through the protective net 10. The air pump 11 is circulated and cooled inside the housing 6, so that the chamber 1 can be restored to the set low temperature environment in a short time, reducing the time that stem cells are exposed to non-ideal temperatures, thereby avoiding cell activity decline or death. When cooling the chamber 1, the temperature inside the chamber 1 is detected by the temperature sensor 17 and the data is fed back to the controller 18. When the temperature sensor 17 detects that the temperature inside the chamber 1 has dropped to the set value, the controller 18 stops the air pump 11, so that the internal temperature of the chamber 1 remains stable, creating a better preservation environment for the stem cells inside the chamber 1, so as to facilitate short-term storage of stem cells.

[0045] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A portable stem cell culture preservation box, characterized in that: Includes a housing (1), on both sides of the inner wall of the housing (1) are installed with mounting shells (6), and multiple sets of inclined placement boxes (13) are fixedly installed inside the mounting shells (6). Multiple sets of placement slots (14) for placing dry ice are opened in the multiple sets of placement boxes (13). The interior of the mounting shells (6) is separated by multiple sets of placement boxes (13) to extend the forced contact time between air and dry ice. An air pump (11) is fixedly installed at the bottom of the box (1). The air pump (11) has two sets of extraction pipes (12) for drawing air to flow in the circulation channel. The extraction pipes (12) are connected to the mounting shell (6). The air pump (11) has an exhaust pipe (24) for air discharge.

2. The portable stem cell culture preservation box according to claim 1, characterized in that: The inner walls of both sides of the box (1) are fixedly installed with a second support plate (5). The bottom of the mounting shell (6) is placed on the top of the second support plate (5). The two sides of the mounting shell (6) are fixedly connected with mounting seats (8). The inner walls of both sides of the box (1) are fixedly installed with multiple sets of fixing blocks (7) corresponding to the mounting seats (8). The mounting seats (8) are fixedly installed in the fixing blocks (7) by fixing screws.

3. The portable stem cell culture preservation box according to claim 2, characterized in that: The bottom of the mounting housing (6) is connected to a connecting pipe (25), which is inserted into the second support plate (5). The lower end of the connecting pipe (25) is inserted into the inside of the extraction tube (12). A sealing gasket is provided at the bottom of the connecting pipe (25), and the sealing gasket is pressed against the upper end of the extraction tube (12).

4. The portable stem cell culture preservation box according to claim 1, characterized in that: A fixing plate (9) is fixedly installed on the top of the mounting shell (6), a protective net (10) is provided inside the fixing plate (9), and an interception net plate (23) for intercepting dry ice when it falls is fixedly installed inside the mounting shell (6).

5. A portable stem cell culture preservation box according to claim 1, characterized in that: The inner wall of the box (1) is fixedly installed with a first support plate (2), and the top of the first support plate (2) is fixedly installed with a test tube placement foam seat (3) for placing test tubes. The inner wall of the box (1) is fixedly installed with a positioning frame (4) for positioning test tubes, and the positioning frame (4) has multiple sets of insertion holes (22) for inserting test tubes.

6. A portable stem cell culture preservation box according to claim 5, characterized in that: The end of the exhaust pipe (24) extends between the first support plate (2) and the positioning frame (4), and the positioning frame (4) has multiple sets of flow holes (16) for air flow.

7. A portable stem cell culture preservation box according to claim 1, characterized in that: It also includes a sealing cover (15) fixedly installed on the top of the box (1), the bottom of the sealing cover (15) is provided with a sealing gasket (20), the top of the box (1) is provided with a sealing groove (21) that is compatible with the sealing groove (21), and the sealing gasket (20) is pressed into the sealing groove (21).

8. A portable stem cell culture preservation box according to claim 7, characterized in that: Limit switches (19) are fixedly installed on both inner walls of the box (1). The sealing cover (15) presses on the contacts of the limit switch (19). A temperature sensor (17) for detecting the internal temperature of the box (1) is fixedly installed at the bottom of the sealing cover (15). A controller (18) is fixedly installed at the bottom of the box (1). The air pump (11), temperature sensor (17) and limit switch (19) are all electrically connected to the controller (18).

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