Excrement free DNA extraction kit based on nano magnetic beads

Abstract

The utility model relates to the technical field of DNA extraction, in particular to an excrement free DNA extraction kit based on nano magnetic beads, which comprises a kit body, a test tube storage mechanism is arranged on the inner side of the kit body, and a reagent bottle storage mechanism is arranged on the inner side of the kit body. According to the excrement free DNA extraction kit based on the nano magnetic beads, the interior of the kit body is divided into a reagent bottle storage area and a test tube storage area by a partition plate, classified storage is achieved, a T-shaped groove is in sliding fit with a T-shaped block, the position of a placement plate can be flexibly adjusted, the position of the placement plate can be flexibly adjusted, a clamping block is matched with a clamping groove, and movement of a sliding plate is limited; the position of the placing plate is fixed, an experimenter can conveniently store or take reagent bottles above the placing plate, the test tube storage mechanism adapts to test tubes with different lengths by adjusting the position of a stabilizing plate, and stable fixing of the test tubes is achieved.

Description

Technical Field

[0001] This invention relates to the field of DNA extraction technology, and in particular to a fecal free DNA extraction kit based on nanomagnetic beads. Background Technology

[0002] In fecal cell-free DNA extraction experiments based on magnetic nanobeads, DNA extraction technology is a key step in obtaining genetic information from biological samples. This technology utilizes the specific DNA adsorption properties of magnetic nanobeads to separate cell-free DNA from complex fecal samples, providing important data support for disease diagnosis, microbial research, and other fields. As an important tool for storing reagent bottles and test tubes required for experiments, the rationality of the structural design of the kit directly affects the efficiency and accuracy of the experiment. Therefore, a fecal cell-free DNA extraction kit based on magnetic nanobeads is particularly needed.

[0003] Chinese patent CN222409079U, published on January 28, 2025, discloses a reagent kit. The base structure of this kit can exert an upward force on the test tubes, causing the test tubes to extend above the positioning plate for easy retrieval. However, in practical applications, this reagent kit has a relatively fixed and simple storage structure, lacks an effective storage solution for test tubes of different lengths, and lacks space for storing reagent bottles, making it difficult to meet diverse experimental needs. Utility Model Content

[0004] The purpose of this invention is to provide a fecal cell-free DNA extraction kit based on nanomagnetic beads to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a fecal cell-free DNA extraction kit based on nano-magnetic beads, comprising a box body, a hinge rotatably connected to the outer surface of the box body, a box lid rotatably connected to one side surface of the hinge, a partition plate fixedly connected to the inner surface of the box body, a test tube storage mechanism provided on the inner side of the box body, and a reagent bottle storage mechanism provided on the inner side of the box body.

[0006] The reagent bottle storage mechanism includes a fixing plate, which is installed on the inner surface of the box body. A T-shaped groove is formed on the inner surface of the fixing plate. A T-shaped block is slidably connected to the inner surface of the T-shaped groove. A sliding plate is fixedly connected to one side surface of the T-shaped block. A stabilizing block is fixedly connected to one side surface of the sliding plate. A moving groove is formed on the inner surface of the stabilizing block. A limiting rod is fixedly connected to the inner surface of the moving groove. A moving plate is slidably connected to the outer surface of the limiting rod. A locking block is fixedly connected to the lower surface of the moving plate. A locking groove is formed on the upper surface of the box body. A placement plate is fixedly connected to one side surface of the sliding plate.

[0007] Preferably, one side of the partition plate is provided with two sets of symmetrically distributed reagent bottle storage mechanisms, and the other side of the partition plate is provided with a test tube storage mechanism.

[0008] Preferably, there are two sets of fixed plates and T-shaped blocks, three sets of stabilizing blocks, locking blocks and placement plates are arranged at equal intervals on the outer surface of the sliding plate, and two sets of limiting rods are symmetrically arranged on the inner side of the moving groove.

[0009] Preferably, the surface of the placement plate is provided with multiple sets of anti-slip strips at equal intervals, the card block is slidably connected to the card slot, the moving plate is "L" shaped and is slidably connected to the moving groove, and the placement plate is placed at an angle.

[0010] Preferably, the test tube preservation mechanism includes a base, which is installed on the inner surface of the box body. A test tube slot is formed on the upper surface of the base, and a test tube is slidably connected to the inner surface of the test tube slot. A fixing slot is formed on the inner surface of the box body, and a fixing block is slidably connected to the inner surface of the fixing slot. A stabilizing plate is slidably connected to the outer surface of the fixing block. A limiting slot is formed on the inner surface of the stabilizing plate. A spring is fixedly connected to one side surface of the fixing block, and a connecting rod is fixedly connected to one side surface of the fixing block. A limit block is fixedly connected to one end surface of the connecting rod. A limit slot is formed on the inner surface of the stabilizing plate, and a stabilizing slot is formed on the surface of the stabilizing plate. A lifting block is fixedly connected to the upper surface of the stabilizing plate.

[0011] Preferably, the stabilizing plate is provided in three sets, and the fixing groove is provided in multiple sets at equal intervals, and in three rows.

[0012] Preferably, the limiting groove is slidably connected to the fixing block, multiple sets of stabilizing grooves and test tube grooves are provided, the stabilizing grooves and test tube grooves correspond to each other, and the outer dimension of the limiting block matches the inner dimension of the limiting groove.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: This fecal cell-free DNA extraction kit based on nano-magnetic beads has a separator plate that divides the internal part of the box into a reagent bottle storage area and a test tube storage area, achieving classified storage. The sliding fit between the T-shaped groove and the T-shaped block allows for flexible adjustment of the position of the placement plate. The locking block and the locking groove cooperate with each other to restrict the movement of the sliding plate and fix the position of the placement plate, making it convenient for experimental personnel to store or retrieve the reagent bottles on the placement plate. The test tube storage mechanism adapts to test tubes of different lengths by adjusting the position of the stabilizing plate, achieving stable fixation of the test tubes. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the appearance and structure of this utility model;

[0015] Figure 2 This is a schematic diagram of the reagent bottle storage mechanism of this utility model;

[0016] Figure 3 This is a schematic diagram of the test tube preservation mechanism of this utility model;

[0017] Figure 4 This is a schematic diagram of the structure of the limiting block and the limiting groove of this utility model.

[0018] In the diagram: 1. Box body; 2. Hinge; 3. Box lid; 4. Divider plate; 5. Test tube storage mechanism; 501. Base; 502. Test tube slot; 503. Test tube; 504. Fixing slot; 505. Fixing block; 506. Stabilizing plate; 507. Limiting slot; 508. Spring; 509. Connecting rod; 510. Limiting block; 511. Limiting slot; 512. Stabilizing slot; 513. Lifting block; 6. Reagent bottle storage mechanism; 601. Fixing plate; 602. T-shaped slot; 603. T-shaped block; 604. Sliding plate; 605. Stabilizing block; 606. Moving slot; 607. Limiting rod; 608. Moving plate; 609. Locking block; 610. Locking groove; 611. Placement plate; 612. Anti-slip strip. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figure 1-4 This utility model provides a technical solution: a fecal free DNA extraction kit based on nano-magnetic beads, including a box body 1, a hinge 2 rotatably connected to the outer surface of the box body 1, a box cover 3 rotatably connected to one side surface of the hinge 2, a partition plate 4 fixedly connected to the inner surface of the box body 1, a test tube storage mechanism 5 provided inside the box body 1, and a reagent bottle storage mechanism 6 provided inside the box body 1.

[0021] The reagent bottle storage mechanism 6 includes a fixing plate 601, which is installed on the inner surface of the box body 1. A T-shaped groove 602 is formed on the inner surface of the fixing plate 601. A T-shaped block 603 is slidably connected to the inner surface of the T-shaped groove 602. A sliding plate 604 is fixedly connected to one side surface of the T-shaped block 603. A stabilizing block 605 is fixedly connected to one side surface of the sliding plate 604. A moving groove 606 is formed on the inner surface of the stabilizing block 605. A limiting rod 607 is fixedly connected to the inner surface of the moving groove 606. A moving plate 608 is slidably connected to the outer surface of the limiting rod 607. A locking block 609 is fixedly connected to the lower surface of the moving plate 608. A locking groove 610 is formed on the upper surface of the box body 1. A placement plate 611 is fixedly connected to one side surface of the sliding plate 604. With the above structure, when it is necessary to place… When placing or removing reagent bottles, pull the sliding plate 604, and the T-shaped block 603 slides in the T-shaped groove 602, thereby adjusting the placement plate 611 to the appropriate position. After determining the position of the placement plate 611, move the moving plate 608 to slide in the moving groove 606. As the moving plate 608 moves, the locking block 609 fixedly connected below it gradually approaches the locking groove 610 above the box 1. Then, move the sliding plate 604 downward, and the locking block 609 will lock into the locking groove 610, restricting the sliding plate 604 and preventing it from moving further, thus fixing the position of the placement plate 611. At this time, the reagent bottle can be placed on the placement plate 611 for storage or removal. After storage or removal is completed, move the sliding plate 604 upward, so that the locking block 609 disengages from the locking groove 610, and then push the sliding plate 604 back to its original position to prepare for the next use.

[0022] Furthermore, two sets of symmetrically distributed reagent bottle storage mechanisms 6 are provided on one side of the partition plate 4, and a test tube storage mechanism 5 is provided on the other side of the partition plate 4. Through the above structure, when in use, the partition plate 4 clearly divides the internal space of the box 1 into a reagent bottle storage area and a test tube storage area. Experimenters can place the reagent bottles in the reagent bottle storage mechanism 6 in an orderly manner and place the test tubes 503 in the test tube storage mechanism 5, so as to realize the classified storage of reagent bottles and test tubes 503, avoid mutual interference, reduce errors in retrieval, and facilitate the experimenters to quickly locate the required items.

[0023] Furthermore, two sets of fixing plates 601 and T-shaped blocks 603 are provided, and three sets of stabilizing blocks 605, locking blocks 609, and placement plates 611 are provided at equal intervals on the outer surface of the sliding plate 604. Two sets of limiting rods 607 are symmetrically provided on the inner side of the moving groove 606. With the above structure, during use, the two sets of fixing plates 601 and T-shaped blocks 603 form a double-track support structure to ensure the stability of the sliding plate 604 during movement. The three sets of equally spaced placement plates 611 increase the number of reagent bottles that can be stored. The two sets of symmetrically provided limiting rods 607 ensure that the moving plate 608 will not deviate when sliding, so that the locking blocks 609 can be accurately locked into the slots 610.

[0024] Furthermore, the surface of the placement plate 611 is provided with multiple sets of anti-slip strips 612 at equal intervals. The locking block 609 is slidably connected to the locking groove 610. The moving plate 608 is L-shaped and is slidably connected to the moving groove 606. The placement plate 611 is placed at an angle. With the above structure, when in use, the anti-slip strips 612 increase the friction between the reagent bottle and the placement plate 611 to prevent the reagent bottle from sliding. The tilted placement plate 611 uses gravity to make the reagent bottle automatically adhere to the lower side of the plate surface.

[0025] Furthermore, the test tube preservation mechanism 5 includes a base 501, which is installed on the inner surface of the box body 1. A test tube groove 502 is formed on the upper surface of the base 501. A test tube 503 is slidably connected to the inner surface of the test tube groove 502. A fixing groove 504 is formed on the inner surface of the box body 1. A fixing block 505 is slidably connected to the inner surface of the fixing groove 504. A stabilizing plate 506 is slidably connected to the outer surface of the fixing block 505. A limiting groove 507 is formed on the inner surface of the stabilizing plate 506. A spring 508 is fixedly connected to one side surface of the fixing block 505. A connecting rod 509 is fixedly connected to one side surface of the stabilizing plate 506. A limiting block 510 is fixedly connected to one end surface of the connecting rod 509. A limiting groove 511 is formed on the inner surface of the stabilizing plate 506, and a stabilizing groove 512 is formed on the surface of the stabilizing plate 506. A lifting block 513 is fixedly connected to the upper surface of the stabilizing plate 506. With the above structure, when no test tube 503 is placed, the stabilizing plate 506 is in the initial position, the spring 508 is in the natural state, and the fixing block 505 is located in the fixing groove 504. When the lifting block 513 is pulled upward, the stabilizing plate 506 is moved upward. When the plate moves upward, the stabilizing plate 506 will drive the fixing block 505 to move. During the movement of the fixing block 505 with the stabilizing plate 506, the fixing block 505 is resisted by the inner wall of the fixing groove 504, causing the fixing block 505 to compress the spring 508. The spring 508 stores elastic potential energy. The experimenter can pull the stabilizing plate 506 to a suitable position according to the length of the test tube 503. At this time, the spring 508 is in a compressed state, providing power for subsequent fixation. When the stabilizing plate 506 moves to the suitable position, the lifting block 513 is released, and the spring 508 returns to its original state. The resulting elastic force pushes the fixing block. 505 moves into the fixing groove 504, causing the fixing block 505 to pop out and snap into the corresponding fixing groove 504. The fixing block 505 drives the limiting block 510 to move through the connecting rod 509. The limiting block 510 slides in the limiting groove 511, restricting the movement of the fixing block 505 and ensuring that the fixing block 505 can only slide in a straight line in the limiting groove 507 without rotation. This avoids affecting the normal operation and stability of the spring 508 due to rotation. After the stabilizing plate 506 is fixed, the test tube 503 is passed through the stabilizing groove 512 on the surface of the stabilizing plate 506 and placed in the test tube groove 502.

[0026] Furthermore, the stabilizer plate 506 is provided in three sets, and the fixing groove 504 is provided in multiple sets at equal intervals and in three rows. With the above structure, the three sets of stabilizer plates 506 can be freely adjusted during use. Together with the three rows of multiple sets of equally spaced fixing grooves 504, it provides a variety of adaptation options for test tubes 503 of different lengths, thereby improving the versatility and practicality of the reagent kit.

[0027] Furthermore, the limiting groove 507 is slidably connected to the fixing block 505, and multiple sets of stabilizing grooves 512 and test tube grooves 502 are provided. The stabilizing grooves 512 and test tube grooves 502 correspond to each other, and the outer dimension of the limiting block 510 matches the inner dimension of the limiting groove 511. With the above structure, in use, multiple sets of corresponding stabilizing grooves 512 and test tube grooves 502 can fix multiple test tubes 503 at the same time and achieve precise positioning of the test tubes 503. The limiting block 510 and the limiting groove 511 engage to prevent the fixing block 505 from shifting or rotating, thereby enhancing the overall structural stability.

[0028] Working principle: When test tube 503 is not placed, the stabilizing plate 506 is in its initial position, the spring 508 is in its natural state, and the fixing block 505 is located in the fixing groove 504. When the lifting block 513 is pulled upward, causing the stabilizing plate 506 to move upward, the stabilizing plate 506 will move the fixing block 505. During the movement of the fixing block 505 with the stabilizing plate 506, the fixing block 505 is resisted by the inner wall of the fixing groove 504, causing the fixing block 505 to compress the spring 508. The spring 508 stores elastic potential energy. The experimenter can pull the stabilizing plate 506 according to the length of the test tube 503. When the plate 506 reaches the appropriate position, the spring 508 is compressed, providing power for subsequent fixing. After the stabilizing plate 506 moves to the appropriate position, the lifting block 513 is released, and the spring 508 returns to its original state. The resulting elastic force pushes the fixing block 505 into the fixing groove 504, causing the fixing block 505 to pop out and engage with the corresponding fixing groove 504. The fixing block 505, through the connecting rod 509, drives the limiting block 510 to move. The limiting block 510 slides within the limiting groove 511, restricting the movement of the fixing block 505 and ensuring that the fixing block 505 can only slide in a straight line within the limiting groove 507. Rotation may occur. To prevent the rotation from affecting the normal operation and stability of the spring 508, after the stabilizing plate 506 is fixed, the test tube 503 is passed through the stabilizing groove 512 on the surface of the stabilizing plate 506 and placed in the test tube slot 502. When it is necessary to place or remove the reagent bottle, pull the sliding plate 604, and the T-shaped block 603 slides in the T-shaped groove 602, thereby driving the placement plate 611 to adjust to the appropriate position. After the position of the placement plate 611 is determined, move the moving plate 608 to slide in the moving groove 606. As the moving plate 608 moves, the locking block 609 fixedly connected below it gradually moves closer to the box body. Move the upper slot 610, then move the sliding plate 604 downwards. The locking block 609 will lock into the slot 610, restricting the sliding plate 604 and preventing it from moving further, thus fixing the position of the placement plate 611. At this time, the reagent bottle can be placed on the placement plate 611 for storage or retrieval. After storage or retrieval, move the sliding plate 604 upwards to disengage the locking block 609 from the slot 610, and then push the sliding plate 604 back to its original position to prepare for the next use. This completes the use of a fecal free DNA extraction kit based on nanomagnetic beads.

[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A fecal cell-free DNA extraction kit based on magnetic nanobeads, comprising a housing (1), characterized in that: The outer surface of the box (1) is rotatably connected to a hinge (2), and one side surface of the hinge (2) is rotatably connected to a box lid (3). The inner surface of the box (1) is fixedly connected to a partition plate (4). The inner side of the box (1) is provided with a test tube storage mechanism (5) and a reagent bottle storage mechanism (6). The reagent bottle storage mechanism (6) includes a fixing plate (601), which is installed on the inner surface of the box (1). A T-shaped groove (602) is provided on the inner surface of the fixing plate (601). A T-shaped block (603) is slidably connected to the inner surface of the T-shaped groove (602). A sliding plate (604) is fixedly connected to one side surface of the T-shaped block (603). A stabilizing block (605) is fixedly connected to one side surface of the sliding plate (604). The inner surface of the stabilizing block (605) is provided with a moving groove (606), the inner surface of the moving groove (606) is fixedly connected with a limiting rod (607), the outer surface of the limiting rod (607) is slidably connected with a moving plate (608), the lower surface of the moving plate (608) is fixedly connected with a locking block (609), the upper surface of the box (1) is provided with a locking groove (610), and one side surface of the sliding plate (604) is fixedly connected with a placement plate (611).

2. The fecal cell-free DNA extraction kit based on magnetic nanobeads according to claim 1, characterized in that: Two sets of symmetrically distributed reagent bottle storage mechanisms (6) are provided on one side of the partition plate (4), and a test tube storage mechanism (5) is provided on the other side of the partition plate (4).

3. The fecal cell-free DNA extraction kit based on magnetic nanobeads according to claim 1, characterized in that: Two sets of the fixed plate (601) and T-shaped block (603) are provided. Three sets of the stabilizing block (605), the locking block (609) and the placement plate (611) are provided at equal intervals on the outer surface of the sliding plate (604). Two sets of the limiting rod (607) are symmetrically provided on the inner side of the moving groove (606).

4. The fecal cell-free DNA extraction kit based on magnetic nanobeads according to claim 1, characterized in that: The surface of the placement plate (611) is provided with multiple sets of anti-slip strips (612) at equal intervals. The card block (609) is slidably connected to the card slot (610). The moving plate (608) is "L" shaped and is slidably connected to the moving groove (606). The placement plate (611) is placed at an angle.

5. The fecal cell-free DNA extraction kit based on magnetic nanobeads according to claim 1, characterized in that: The test tube preservation mechanism (5) includes a base (501), which is installed on the inner surface of the box (1). A test tube slot (502) is provided on the upper surface of the base (501). A test tube (503) is slidably connected to the inner surface of the test tube slot (502). A fixing slot (504) is provided on the inner surface of the box (1). A fixing block (505) is slidably connected to the inner surface of the fixing slot (504). A stabilizing plate (506) is slidably connected to the outer surface of the fixing block (505). The inner surface of (506) is provided with a limiting groove (507), a spring (508) is fixedly connected to one side surface of the fixing block (505), a connecting rod (509) is fixedly connected to one side surface of the fixing block (505), a limiting block (510) is fixedly connected to one end surface of the connecting rod (509), a limiting groove (511) is provided on the inner surface of the stabilizing plate (506), a stabilizing groove (512) is provided on the surface of the stabilizing plate (506), and a lifting block (513) is fixedly connected to the upper surface of the stabilizing plate (506).

6. The fecal cell-free DNA extraction kit based on magnetic nanobeads according to claim 5, characterized in that: The stabilizing plate (506) is provided in three sets, and the fixing groove (504) is provided in multiple sets at equal intervals and in three rows.

7. The fecal cell-free DNA extraction kit based on magnetic nanobeads according to claim 5, characterized in that: The limiting groove (507) is slidably connected to the fixing block (505). Multiple sets of the stabilizing groove (512) and the test tube groove (502) are provided. The stabilizing groove (512) and the test tube groove (502) correspond to each other. The outer dimension of the limiting block (510) matches the inner dimension of the limiting groove (511).

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