Automatic cell collecting device

Abstract

The utility model belongs to the technical field of cell culture, and particularly relates to an automatic cell collecting device which comprises a rack and a sealing cover arranged on the rack, a normally closed butt joint window is arranged on one side of the sealing cover, and the butt joint window can be connected with a culture box; a culture dish can be placed in the culture box; the first mechanical arm is arranged on the operation platform; the liquid collecting mechanism is arranged on the operation platform, the liquid input mechanism is arranged on the operation platform, a first cover opening assembly is further arranged beside the liquid input mechanism, and the centrifugal collecting mechanism is arranged on the operation platform. By arranging the butt joint window, the first mechanical arm, the liquid collection mechanism, the liquid input mechanism and the centrifugal collection mechanism, the cell collection process can be fully automatically completed, the automation degree is high, the risk of artificial secondary pollution is reduced, meanwhile, a production line can be synthesized with a cell inoculation device, the first mechanical arm is shared, the size of the machine is reduced, and the production cost is reduced. The occupied area is small, the cost is low, and the structure is simpler.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, and in particular relates to an automated cell collection device. Background Technology

[0002] A cell factory is a cell culture device that maximizes the culture surface area within a limited space, thus saving space. It can be used for industrial-scale production such as vaccines, monoclonal antibodies, or biopharmaceuticals, and is particularly suitable for adherent cells. It can also be used for suspension culture, and it does not alter the cell growth kinetics when scaling up from laboratory scale, making scale-up simple, easy, with low contamination risk, and space-saving. The collection device is an important component of a cell factory.

[0003] Most existing automated cell collection devices are single production lines, which occupy a large area and have high costs. Some collection devices are small equipment, but their automation level is not high, and a lot of work needs to be done manually, which can cause secondary pollution. Utility Model Content

[0004] The purpose of this invention is to provide an automated cell collection device, which aims to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides an automated cell collection device, including a frame and a sealing cover disposed on the frame. The sealing cover and the top side of the frame form a closed operating space. An operating platform is provided within the operating space. A normally closed docking window is provided on one side of the sealing cover, which can be connected to an incubator. A culture dish can be placed inside the incubator, and the incubator can provide a culture environment for the culture dish.

[0006] A first robotic arm is mounted on the operating platform and can move the culture dish; the operating platform is also equipped with a cell inoculation device, which shares the first robotic arm with the cell collection device.

[0007] A liquid collection mechanism is located on the operating platform, next to the first robotic arm;

[0008] A liquid input mechanism is provided on the operating platform and next to the first robotic arm; a first lid opening assembly is also provided next to the liquid input mechanism, which is used to open and close the lid of the culture dish;

[0009] A centrifugal collection mechanism, which is located on the operating platform, is used to collect target cells.

[0010] Optionally, the liquid collection mechanism includes a supernatant collection component, a cleaning waste liquid collection component, and a suspension collection component; each of the supernatant collection component, the cleaning waste liquid collection component, and the suspension collection component includes a support base and a collection funnel disposed on the support base, the collection funnel is connected to a conduit, the supernatant collection component is connected to the liquid input mechanism through the conduit, the cleaning waste liquid collection component is connected to a waste liquid tank through the conduit, and the suspension collection component is connected to the centrifugal collection mechanism through the conduit.

[0011] Optionally, the liquid input mechanism includes a cleaning fluid output pipe, a digestive fluid output pipe, and a supernatant output pipe; the other end of the cleaning fluid output pipe is connected to a cleaning fluid bag, the digestive fluid output pipe is connected to a digestive fluid bag, and the supernatant output pipe is connected to the conduit of the supernatant collection assembly.

[0012] Optionally, the centrifugal collection mechanism includes a centrifuge, a second robotic arm, a second capping assembly, a rotating disk, a blow-resuspend assembly, a suspension input assembly, a cleaning fluid pipetting assembly, and a centrifuge bottle pipetting assembly; the second capping assembly, the blow-resuspend assembly, the suspension input assembly, the cleaning fluid pipetting assembly, and the centrifuge bottle pipetting assembly are arranged circumferentially around the rotating disk.

[0013] Optionally, the rotating disk includes a placement rack and a rotating motor connected to the placement rack. The rotating motor can drive the placement rack to rotate. The placement rack has multiple placement positions for placing centrifuge bottles.

[0014] Optionally, the centrifugal collection mechanism further includes a waste liquid collector and a solid waste collector; the second robotic arm can pick up the centrifuge bottle and pour the waste liquid into the waste liquid collector.

[0015] Optionally, the blow-and-suspension assembly includes a first base and a syringe; the first base is provided with a first lifting module, the first lifting module is provided with a pushing component, the pushing component is connected to the syringe, and the pushing component is used to push the syringe.

[0016] Optionally, one end of the docking window is connected to the operating space, and the other end is connected to the incubator. A barcode scanner is provided in the docking window, which is used to identify and input the information of the culture dish.

[0017] Optionally, the centrifuge bottle pipetting assembly includes a second base and a second lifting module disposed on the second base; the second lifting module is provided with a pipetting funnel, which is disposed above the centrifuge bottle.

[0018] Optionally, it also includes a petri dish transport assembly; which is disposed on the frame, and the operating platform has an opening for the petri dish to pass through. The petri dish transport assembly is disposed below the opening. The petri dish transport assembly includes a third lifting module, a pushing module, and a conveyor belt; the first robotic arm places the used petri dish into the third lifting module through the opening and lowers it to the same height as the conveyor belt, and the pushing module pushes the petri dish onto the conveyor belt for transport.

[0019] Compared with the prior art, the above-mentioned one or more technical solutions in the automated cell collection device provided by the present invention have at least one of the following technical effects:

[0020] By setting up a docking window, a first robotic arm, a liquid collection mechanism, a liquid input mechanism, and a centrifugal collection mechanism, the cell collection process can be completed fully automatically without human intervention. The high degree of automation reduces the risk of secondary contamination. At the same time, the cell inoculation device and the cell collection device are integrated into a production line, sharing the first robotic arm, which saves the machine's volume, occupies less space, has lower cost, and a simpler structure. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the structure of this utility model.

[0023] Figure 2 This is a partial structural diagram of the present utility model.

[0024] Figure 3 This is a partial structural diagram of the present utility model.

[0025] Figure 4 This is a schematic diagram of the structure of this utility model from another angle.

[0026] Figure 5 This is a schematic diagram of the docking window and incubator structure.

[0027] Figure 6 This is a schematic diagram of the structure of the blow-and-suspension assembly.

[0028] Figure 7 This is a schematic diagram of the petri dish transport assembly.

[0029] The following are the labeling elements in the figure:

[0030] 100. Frame; 110. Sealing cover; 120. Operating platform; 121. Opening; 130. Docking window; 131. Barcode scanner;

[0031] 200. Incubator; 210. Petri dish;

[0032] 300. The first robotic arm;

[0033] 400. Liquid collection mechanism; 410. Supernatant collection assembly; 420. Cleaning waste liquid collection assembly; 430. Suspension collection assembly; 431. Support base; 432. Collection funnel;

[0034] 500. Liquid input mechanism; 510. Cleaning fluid output pipe; 520. Digestive fluid output pipe; 530. Supernatant output pipe; 540. Cleaning fluid bag; 550. Digestive fluid bag;

[0035] 610. Centrifuge; 620. Second robotic arm; 630. Second cap opening assembly; 640. Rotary disc; 641. Placement rack; 642. Rotary motor; 643. Centrifuge bottle; 650. Blow-and-resuspend assembly; 651. First base; 652. Syringe; 653. First lifting module; 654. Pushing assembly; 660. Suspension input assembly; 670. Cleaning fluid pipetting assembly; 680. Centrifuge bottle pipetting assembly; 681. Second base; 682. Second lifting module; 683. Pipetting funnel; 691. Waste liquid collector; 692. Solid waste collector;

[0036] 710. First lid opening assembly; 720. Settling box; 730. Petri dish transport assembly; 731. Third lifting module; 732. Pushing module; 733. Conveyor belt; 740. Large continuous centrifuge; 750. Small continuous centrifuge; 760. Tube filling equipment; 770. Bag filling equipment;

[0037] 800. Cell inoculation device. Detailed Implementation

[0038] The embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the embodiments of the present invention, and should not be construed as limiting the present invention.

[0039] In the description of the embodiments of this utility model, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the embodiments of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0040] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of embodiments of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0041] In this embodiment of the invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention according to the specific circumstances.

[0042] In one embodiment of this utility model, according to Figure 1-7As shown, the system includes a frame 100 and a sealing cover 110 mounted on the frame 100. The sealing cover 110 and the top side of the frame 100 form a closed operating space. An operating platform 120 is provided within the operating space. A normally closed docking window 130 is provided on one side of the sealing cover 110, which can be connected to an incubator 200. The incubator 200 can hold a culture dish 210 and provides a culture environment for the culture dish 210. A first robotic arm 300 is mounted on the operating platform 120 and can move the culture dish 210. The operating platform 120... The system also includes a cell seeding device 800, which shares a first robotic arm 300 with the cell collection device; a liquid collection mechanism 400, which is located on the operating platform 120 and next to the first robotic arm 300; a liquid input mechanism 500, which is located on the operating platform 120 and next to the first robotic arm 300; a first cap opening assembly 710, which is used to open and close the cap of the culture dish 210, is also located next to the liquid input mechanism 500; and a centrifugation collection mechanism, which is located on the operating platform 120 and is used to collect target cells.

[0043] Specifically, by setting up a docking window 130, a first robotic arm 300, a liquid collection mechanism 400, a liquid input mechanism 500, and a centrifugal collection mechanism, the cell collection process can be completed fully automatically without human intervention. The high degree of automation reduces the risk of secondary contamination caused by human error. At the same time, the cell inoculation device 800 and the cell collection device are integrated into a production line, sharing the first robotic arm 300, which saves the machine's volume, occupies less space, has lower cost, and a simpler structure.

[0044] In another embodiment of this utility model, according to Figure 1-3 As shown, one end of the docking window 130 is connected to the operating space, and the other end is connected to the incubator 200. A barcode scanner 131 is installed inside the docking window 130. The barcode scanner 131 is used to identify and input information of the culture dish 210.

[0045] In another embodiment of this utility model, according to Figure 2-6As shown, the liquid collection mechanism 400 includes a supernatant collection component 410, a cleaning waste liquid collection component 420, and a suspension collection component 430. Each of these components includes a support base 431 and a collection funnel 432 mounted on the support base 431. The collection funnel 432 is connected to a conduit. The supernatant collection component 410 is connected to a liquid input mechanism 500 via the conduit, the cleaning waste liquid collection component 420 is connected to a waste liquid tank via the conduit, and the suspension collection component 430 is connected to a centrifugal collection mechanism via the conduit. The liquid input mechanism 500 includes a cleaning liquid output pipe 510, a digestive liquid output pipe 520, and a supernatant output pipe 530. The other end of the cleaning liquid output pipe 510 is connected to a cleaning liquid bag 540, the digestive liquid output pipe 520 is connected to a digestive liquid bag 550, and the supernatant output pipe 530 is connected to the conduit of the supernatant collection component 410. It also includes a settling chamber 720, which is used to provide a stable settling environment.

[0046] Specifically, the culture incubator 200, after cultivation, is transported by a transfer trolley to the docking window 130. The incubator 200 has a telescopic device that allows the support plate carrying the culture dish 210 to extend out of the incubator 200 and into the docking window 130. The first robotic arm 300 can directly lift the culture dish 210 from the support plate, first transport it to the first capping assembly 710 to open the cap of the culture dish 210, then transport it to the supernatant collection assembly 410 to pour out the supernatant, move it to the cleaning solution output pipe 510 to add cleaning solution, and then move it to the... The washing liquid collection assembly 420 is used to pour out the washing liquid, which is then moved to the digestion liquid output tube 520 to add digestion liquid. After that, it is moved to the first cap opening assembly 710 to close the cap and placed in the settling box 720 to stand. The digestion liquid causes the cells to separate from the inner wall of the culture dish 210. Then, it is moved to the first cap opening assembly 710 to open the cap, and moved to the supernatant output tube 530 to add supernatant, which stops the digestion of cells in the culture dish 210. Finally, it is moved to the suspension collection assembly 430 to pour out the suspension in the culture dish 210.

[0047] In another embodiment of this utility model, according to Figure 2-6As shown, the centrifugal collection mechanism includes a centrifuge 610, a second robotic arm 620, a second capping assembly 630, a rotating disk 640, a resuspension assembly 650, a suspension input assembly 660, a cleaning fluid transfer assembly 670, and a centrifuge bottle transfer assembly 680. The second capping assembly 630, the resuspension assembly 650, the suspension input assembly 660, the cleaning fluid transfer assembly 670, and the centrifuge bottle transfer assembly 680 are arranged circumferentially around the rotating disk 640. The rotating disk 640 includes a placement rack 641 and a rotation motor 642 connected to the placement rack 641. The rotation motor 642 can drive the placement rack 641 to rotate. The placement rack 641 has multiple placement positions for corresponding placement of centrifuge bottles 643. The centrifugal collection mechanism also includes a waste liquid collector 691 and a solid waste collector 692. The second robotic arm 620 can pick up the centrifuge bottles 643 and pour the waste liquid into the waste liquid collector 691. The blow-and-resuspend assembly 650 includes a first base 651 and a syringe 652; the first base 651 is provided with a first lifting module 653, the first lifting module 653 is provided with a pushing assembly 654, the pushing assembly 654 is connected to the syringe 652, and the pushing assembly 654 is used to push the syringe 652. The centrifuge bottle pipetting assembly 680 includes a second base 681 and a second lifting module 682 provided on the second base 681; the second lifting module 682 is provided with a pipetting funnel 683, which is located above the centrifuge bottle 643.

[0048] Specifically, the suspension input component 660 and the suspension collection component 430 are connected via conduits. The second robotic arm 620 pre-moves empty centrifuge bottles 643 to the second capping component 630 to open the caps and place them back on the rotating disk 640. The suspension input component 660 quantitatively adds the suspension to the centrifuge bottles 643. The rotating disk 640 rotates, adding the suspension to all the centrifuge bottles 643 on the rotating disk 640 in equal portions. The second robotic arm 620 picks up the centrifuge bottles 643, moves them to the second capping component 630 to close the caps, and places them in the centrifuge 610 for centrifugation. After centrifugation, the second robotic arm 620 sequentially removes the centrifuge bottles 643, moves them to the second capping component 630 to open the caps, pours the waste liquid into the waste liquid collector 691, and places them on the rotating disk. The rotating disk rotates. Cleaning solution is added sequentially through the cleaning solution pipetting assembly 670. The turntable rotates, and the liquid is resuspended by the resuscitation assembly 650. The turntable rotates again, and the liquid is transferred sequentially from three centrifuge bottles 643 into one centrifuge bottle 643 using the centrifuge bottle pipetting assembly 680 and the second robotic arm 620. The empty waste bottle is capped and placed in the solid waste collector 692. The second robotic arm 620 moves the full centrifuge bottle 643 to the second capping assembly 630, caps it, and places it in the centrifuge 610 for centrifugation. After centrifugation, the second robotic arm 620 removes the centrifuge bottle 643, transports it to the second capping assembly 630 for opening, and pours the waste liquid into the waste liquid collector 691. Finally, the bottle is moved back to the second capping assembly 630, capped, and placed on the rotating tray 640, completing cell collection. Subsequent aliquoting is then performed.

[0049] In another embodiment of this utility model, according to Figure 2 and 7 As shown, it also includes a culture dish transport assembly 730; it is mounted on the frame 100, and the operating platform 120 has an opening 121 through which the culture dish 210 can pass. The culture dish transport assembly 730 is located below the opening 121. The culture dish transport assembly 730 includes a third lifting module 731, a pushing module 732, and a conveyor belt 733. The first robotic arm 300 places the empty culture dish 210 into the third lifting module 731 through the opening 121 and lowers it to the same height as the conveyor belt 733. The pushing module 732 then pushes the culture dish 210 onto the conveyor belt 733 for transport. Specifically, the first robotic arm 300 places the culture dish 210 with the emptied suspension into the opening 121, the third lifting module 731 catches the culture dish 210 and lowers it to the conveyor belt 733, and the pushing module 732 pushes the culture dish 210 onto the conveyor belt 733 for transport.

[0050] Furthermore, the push module 732 includes a push plate and a linear screw module.

[0051] In another embodiment of this utility model, such as Figure 1As shown, it also includes a continuous flow collection system, which includes a large continuous centrifuge 740 and a small continuous centrifuge 750, as well as a tube filling device 760 and a bag filling device 770.

[0052] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of this utility model. It should not be construed that the specific implementation of this utility model is limited to these descriptions. For those skilled in the art, the architectural form of this utility model can be flexibly varied without departing from its concept, and a series of products can be derived. Any simple deductions or substitutions should be considered as falling within the patent protection scope defined by the submitted claims.

Claims

1. An automated cell collection device, characterized in that, The device includes a frame and a sealing cover mounted on the frame. The sealing cover and the top side of the frame form a closed operating space. An operating platform is provided in the operating space. One side of the sealing cover has a normally closed docking window that can be connected to an incubator. The incubator can hold petri dishes and provide a culture environment for the petri dishes. A first robotic arm is mounted on the operating platform and can move the culture dish; the operating platform is also equipped with a cell inoculation device, which shares the first robotic arm with the cell collection device. A liquid collection mechanism is located on the operating platform, next to the first robotic arm; A liquid input mechanism is provided on the operating platform and next to the first robotic arm; a first lid opening assembly is also provided next to the liquid input mechanism, which is used to open and close the lid of the culture dish; A centrifugal collection mechanism, which is located on the operating platform, is used to collect target cells.

2. The automated cell collection device according to claim 1, characterized in that, The liquid collection mechanism includes a supernatant collection component, a cleaning waste liquid collection component, and a suspension collection component; each of the supernatant collection component, the cleaning waste liquid collection component, and the suspension collection component includes a support base and a collection funnel disposed on the support base. The collection funnel is connected to a conduit. The supernatant collection component is connected to the liquid input mechanism through the conduit. The cleaning waste liquid collection component is connected to a waste liquid tank through the conduit. The suspension collection component is connected to the centrifugal collection mechanism through the conduit.

3. The automated cell collection device according to claim 2, characterized in that, The liquid input mechanism includes a cleaning fluid output pipe, a digestive fluid output pipe, and a supernatant output pipe; the other end of the cleaning fluid output pipe is connected to a cleaning fluid bag, the digestive fluid output pipe is connected to a digestive fluid bag, and the supernatant output pipe is connected to the conduit of the supernatant collection assembly.

4. The automated cell collection device according to claim 1, characterized in that, The centrifugal collection mechanism includes a centrifuge, a second robotic arm, a second cap opening assembly, a rotating disk, a blow-resuspend assembly, a suspension input assembly, a cleaning fluid pipetting assembly, and a centrifuge bottle pipetting assembly; the second cap opening assembly, the blow-resuspend assembly, the suspension input assembly, the cleaning fluid pipetting assembly, and the centrifuge bottle pipetting assembly are arranged circumferentially around the rotating disk.

5. The automated cell collection device according to claim 4, characterized in that, The rotating disk includes a placement rack and a rotating motor connected to the placement rack. The rotating motor can drive the placement rack to rotate. The placement rack has multiple placement positions for placing centrifuge bottles.

6. The automated cell collection device according to claim 5, characterized in that, The centrifugal collection mechanism also includes a waste liquid collector and a solid waste collector; the second robotic arm can pick up the centrifuge bottle and pour the waste liquid into the waste liquid collector.

7. The automated cell collection device according to claim 4, characterized in that, The blow-and-suspension assembly includes a first base and a syringe; the first base is provided with a first lifting module, the first lifting module is provided with a pushing component, the pushing component is connected to the syringe, and the pushing component is used to push the syringe.

8. The automated cell collection device according to claim 1, characterized in that, One end of the docking window is connected to the operating space, and the other end is connected to the incubator. A barcode scanner is provided in the docking window, which is used to identify and record the information of the culture dish.

9. The automated cell collection device according to claim 5, characterized in that, The centrifuge bottle pipetting assembly includes a second base and a second lifting module disposed on the second base; The second lifting module is equipped with a pipetting funnel, which is located above the centrifuge bottle.

10. The automated cell collection device according to claim 1, characterized in that, It also includes a petri dish transport assembly and a settling box; the petri dish transport assembly and the settling box are mounted on the frame, the settling box is used to provide a stable settling environment, the operating platform is provided with an opening for the petri dish to pass through, the petri dish transport assembly is located below the opening, the petri dish transport assembly includes a third lifting module, a pushing module and a conveyor belt; the first robotic arm places the used petri dish into the third lifting module through the opening and lowers it to the same height as the conveyor belt, the pushing module pushes the petri dish onto the conveyor belt for transport.

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