High-pressure homogenizing device for protein purification instrument suitable for viscous sample

Abstract

The utility model relates to the technical field of high-pressure homogenate, in particular to a high-pressure homogenate device for a protein purification instrument suitable for a viscous sample, which comprises a control console, a first sliding rod, a first fixed seat and an electric stirring frame, the first sliding rod is arranged on the control console in a sliding manner, and the first fixed seat is rotatably arranged on the first sliding rod; and an electric stirring frame is mounted on the first fixed seat. A first fixing seat is rotated to drive an electric stirring frame, a second fixing seat and all parts above the first fixing seat to rotate, the positions of the first fixing seat and the second fixing seat are adjusted, a second sliding rod is located above a homogenizing cylinder, and then a telescopic rod of an electric push rod is controlled to stretch and retract; when the homogenizer works, the homogenizer drives a second fixing seat, a second sliding rod, a first elastic piece, a mounting plate and a cleaning sponge to move up and down, so that the cleaning sponge automatically cleans the inner wall of the homogenizer, residues in the homogenizer are prevented from being mixed with subsequent materials needing to be homogenized, and the purity of a sample is guaranteed.

Description

Technical Field

[0001] This utility model relates to the field of high-pressure homogenization technology, and in particular to a high-pressure homogenization device for protein purification instruments suitable for viscous samples. Background Technology

[0002] A protein purification instrument is a laboratory device specifically designed to separate and purify target proteins from complex mixtures. Before using a protein purification instrument for protein separation and purification, the original sample needs to be homogenized under high pressure to ensure that the target protein can be effectively extracted and is suitable for subsequent purification steps.

[0003] Patent CN218924493U discloses a homogenizing tank for high-pressure chromatography packing. It includes an installation platform, a tank body, a hydraulic telescopic rod, and a multi-stage softening homogenizing assembly. The tank body is welded to the top of the installation platform via a support frame. The hydraulic telescopic rod passes through one side of the tank body, with its fixed end welded to the top of the installation platform via a reinforcing rod, and its telescopic end located inside the tank body. Using this patent, the homogenizing agent can be introduced into the water receiving seat through a flexible conduit, and homogenization occurs sequentially as the multi-stage softening homogenizing assembly moves. However, the aforementioned prior art requires manual cleaning of the residue inside the tank after homogenization. Manual cleaning is prone to incomplete cleaning, leading to cross-contamination between batches and affecting purity.

[0004] Therefore, there is a need for a high-pressure homogenizer for protein purification instruments with self-cleaning function, suitable for viscous samples. Utility Model Content

[0005] To overcome the shortcomings of existing patents, which require manual cleaning of residues in the container after homogenization, and which are prone to incomplete cleaning leading to cross-contamination between batches and affecting purity, this invention provides a high-pressure homogenizing device for protein purification instruments with self-cleaning function, suitable for viscous samples.

[0006] To address the aforementioned issues, this utility model employs the following technical solution: a high-pressure homogenizing device for protein purification instruments suitable for viscous samples, comprising a control console, a homogenizing cylinder, a first slide rod, an electric push rod, a first fixed base, and an electric stirring frame. The first slide rod is slidably mounted on the control console, and the first fixed base is rotatably mounted on the first slide rod. The electric stirring frame is mounted on the first fixed base. The homogenizing cylinder is held in place on the control console. The electric push rod is mounted on the control console. The first slide rod is mounted on the telescopic rod of the electric push rod. The control console is electrically connected to the electric stirring frame and the electric push rod. The device also includes a second fixed base, a second slide rod, a first elastic element, a mounting plate, and a cleaning sponge. The second fixed base is fixedly connected to the first fixed base. The second slide rod is slidably mounted on the second fixed base. The mounting plate is fixedly connected to the second slide rod, and a cleaning sponge is embedded in the mounting plate. The first elastic element is sleeved on the second slide rod, and both ends of the first elastic element are connected to the second fixed base and the second slide rod, respectively.

[0007] In a preferred embodiment of the present invention, the device further includes a fixed plate, a third slide rod, a locking block, and a second elastic element. The fixed plate is fixedly connected to the control panel. Two third slide rods are slidably arranged on the fixed plate. A locking block is fixedly connected to each of the third slide rods. A second elastic element is sleeved on the third slide rods. The two ends of the second elastic element are respectively connected to the fixed plate and the locking block. The two locking blocks are respectively locked into the first fixed seat and the second fixed seat.

[0008] In a preferred embodiment of the present invention, the device further includes a pressure cylinder, pressure plates, a pressure pump, and a discharge pipe. The pressure pump is installed on the homogenizing cylinder, the pressure cylinder is placed on the control console, the pressure cylinder is connected to the pressure pump, five pressure plates are fixedly connected inside the pressure cylinder, and the discharge pipe is connected to the pressure cylinder.

[0009] In a preferred embodiment of this utility model, the pressure plate has multiple through holes.

[0010] In a preferred embodiment of this utility model, a placement plate is also included, and the placement plate is provided on the control panel.

[0011] In a preferred embodiment of this utility model, the edges of the card block are rounded.

[0012] Compared with the prior art, the present invention has the following technical effects: 1. By rotating the first fixed seat, the electric stirring frame, the second fixed seat and all the components above it are rotated, so that the positions of the first fixed seat and the second fixed seat are reversed, so that the second slide rod is located above the homogenizing cylinder. Then, the extension and retraction of the electric push rod is controlled to drive the second fixed seat, the second slide rod, the first elastic element, the mounting plate and the cleaning sponge to move up and down, so that the cleaning sponge automatically cleans the inner wall of the homogenizing cylinder, thereby avoiding the mixing of the residue in the homogenizing cylinder with the material to be homogenized later, and ensuring the purity of the sample.

[0013] 2. The second elastic element rebounds and drives the third slide bar and the locking block to move upward, so that the locking block is locked into the adjacent fixed seat, thereby locking the two fixed seats in position and preventing the angle of the two fixed seats from easily shifting during use.

[0014] 3. By starting the pressure pump, the pressure pump draws liquid from the homogenizing cylinder, so that the liquid is drawn into the pressure cylinder and separated through the through holes on multiple pressure plates, thereby breaking up the aggregated particles and making the solid particles in the suspension more uniformly dispersed. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention.

[0016] Figure 2 This is a three-dimensional structural diagram of the control console, homogenizing cylinder, and first slide bar of this utility model.

[0017] Figure 3 This is a three-dimensional cross-sectional view of the components of this utility model, such as the electric push rod and the electric stirring rack.

[0018] Figure 4 This is a three-dimensional structural diagram of the second slide bar and the first elastic element of this utility model.

[0019] Figure 5 This is a three-dimensional structural diagram of the components of this utility model, including the placement plate, fixing plate, and third sliding rod.

[0020] Figure 6 This is a three-dimensional cross-sectional view of the card block and the second elastic element of this utility model.

[0021] Figure 7 This is a three-dimensional structural diagram of the control console, pressure cylinder, and pressure plate of this utility model.

[0022] Figure 8 This is a three-dimensional structural diagram of the pressure plate, pressure pump, and discharge pipe of this utility model.

[0023] The components in the attached diagram are labeled as follows: 1. Control console, 2. Homogenizing cylinder, 3. First slide bar, 4. Electric push rod, 5. First fixed base, 6. Electric mixing rack, 7. Second fixed base, 8. Second slide bar, 9. First elastic element, 10. Mounting plate, 11. Cleaning sponge, 12. Placement plate, 13. Fixed plate, 14. Third slide bar, 15. Clamping block, 16. Second elastic element, 17. Pressure cylinder, 18. Pressure plate, 19. Pressure pump, 20. Discharge pipe. Detailed Implementation

[0024] Although this invention may be described with respect to a particular application or industry, those skilled in the art will recognize its broader applicability. Those skilled in the art will understand that terms such as "above," "below," "upward," "downward," etc., are used to describe the drawings and not to indicate a limitation on the scope of the invention as defined by the appended claims. Any numerical designations such as "first" or "second" are merely illustrative and not intended to limit the scope of the invention in any way.

[0025] Example 1: A high-pressure homogenizer for protein purification instruments suitable for viscous samples, see [reference]. Figures 1-8 As shown, the device includes a control console 1, a homogenizing cylinder 2, a first sliding rod 3, an electric push rod 4, a first fixed base 5, and an electric mixing frame 6. The first sliding rod 3 is slidably mounted on the upper part of the control console 1, and the first fixed base 5 is rotatably mounted on the upper end of the first sliding rod 3. The electric mixing frame 6 is bolted to the first fixed base 5. The homogenizing cylinder 2 is held in place on the upper part of the control console 1. When the electric mixing frame 6 is started, its mixing components rotate within the homogenizing cylinder 2. The electric push rod 4 is bolted to the upper part of the control console 1, and the first sliding rod 3 is mounted on the telescopic rod of the electric push rod 4. The control console 1 and the electric mixing frame 6... The electric push rod 4 is electrically connected and also includes a second fixed seat 7, a second slide rod 8, a first elastic element 9, a mounting plate 10, and a cleaning sponge 11. The second fixed seat 7 is welded to the rear side of the first fixed seat 5. The second slide rod 8 is slidably mounted on the second fixed seat 7. The mounting plate 10 is fixedly connected to the bottom end of the second slide rod 8. The cleaning sponge 11 is embedded in the mounting plate 10. The first elastic element 9 is sleeved on the second slide rod 8. The two ends of the first elastic element 9 are respectively connected to the second fixed seat 7 and the second slide rod 8. When the mounting plate 10 is inside the homogenizing cylinder 2, the cleaning sponge 11 can contact the inner wall of the homogenizing cylinder 2.

[0026] See Figure 1 , Figure 4 and Figure 5 As shown, it also includes a placement plate 12. The control panel 1 is equipped with a placement plate 12 to hold a container to collect water droplets flowing down the cleaning sponge 11.

[0027] When using this device, first pour the liquid to be homogenized into the homogenizing cylinder 2. Then, start the electric stirring frame 6 via the control panel 1 to homogenize the liquid. After the stirring is complete, turn off the electric stirring frame 6 and remove the slurry. Then, add water into the homogenizing cylinder 2 and start the electric push rod 4. This will extend the telescopic rod of the electric push rod 4, causing the first fixed base 5 and the electric stirring frame 6 to move upwards, allowing the electric stirring frame 6 to leave the homogenizing cylinder 2. Subsequently, by rotating the first fixed base 5, the first fixed base 5 will drive the electric stirring frame 6, the second fixed base 7, and all components above them to rotate, thus reversing the positions of the first fixed base 5 and the second fixed base 7. At the same time, the second fixed base 7 will drive the second sliding rod 8, the first elastic element 9, the mounting plate 10, and the cleaning sponge 11. Move the second slide bar 8 above the homogenizing cylinder 2, then control the extension and retraction of the electric push rod 4 to move the first slide bar 3, the first fixed seat 5, and the second fixed seat 7 up and down. The second fixed seat 7 moves the second slide bar 8, the first elastic element 9, the mounting plate 10, and the cleaning sponge 11 up and down. When the mounting plate 10 contacts the bottom of the homogenizing cylinder 2, the second slide bar 8 is squeezed and the first elastic element 9 is compressed, so that the cleaning sponge 11 automatically cleans the inner wall of the homogenizing cylinder 2, thereby achieving an automatic cleaning effect. This prevents the residue in the homogenizing cylinder 2 from mixing with the materials to be homogenized later, ensuring the purity of the sample. After cleaning, operate the electric push rod 4 and rotate the first fixed seat 5 to reset all components.

[0028] Example 2: Based on Example 1, refer to Figure 1 and Figure 6 As shown, it also includes a fixed plate 13, a third slide rod 14, a locking block 15, and a second elastic element 16. The fixed plate 13 is fixedly connected to the upper part of the control panel 1. The third slide rod 14 is slidably arranged on both the left and right sides of the fixed plate 13. The locking block 15 is fixedly connected to the top of the third slide rod 14. The second elastic element 16 is sleeved on the third slide rod 14. The two ends of the second elastic element 16 are respectively connected to the fixed plate 13 and the locking block 15. The two locking blocks 15 are respectively locked into the first fixed seat 5 and the second fixed seat 7.

[0029] See Figure 6 As shown, the top edge of the locking block 15 is rounded to facilitate its insertion into the first fixing seat 5 and the second fixing seat 7.

[0030] When the first fixed seat 5 and the second fixed seat 7 rotate, the first fixed seat 5 and the second fixed seat 7 respectively press the locking block 15 on themselves. The locking block 15 then drives the third slide rod 14 to move downward, and the second elastic element 16 is compressed accordingly. After the first fixed seat 5 and the second fixed seat 7 exchange positions, the second elastic element 16 rebounds and drives the third slide rod 14 and the locking block 15 to move upward, so that the locking block 15 is locked into the adjacent fixed seat, thereby locking the two fixed seats in position and preventing the angle of the two fixed seats from easily shifting during use.

[0031] See Figure 1 , Figure 7 and Figure 8 As shown, it also includes a pressure cylinder 17, a pressure plate 18, a pressure pump 19, and a discharge pipe 20. The pressure pump 19 is bolted to the bottom of the homogenizing cylinder 2. The pressure cylinder 17 is placed on the control console 1. The pressure cylinder 17 is connected to the pressure pump 19. Five pressure plates 18 are fixedly connected at even intervals inside the pressure cylinder 17. The discharge pipe 20 is connected to the lower part of the pressure cylinder 17.

[0032] See Figure 8 As shown, the pressure plate 18 has multiple through holes.

[0033] After mixing is complete, the pressure pump 19 is started to draw liquid from the homogenizing cylinder 2, so that the liquid is drawn into the pressure cylinder 17 and separated through the through holes on the multiple pressure plates 18, thereby breaking up the agglomerated particles and making the solid particles in the suspension more evenly dispersed. Finally, the liquid is discharged from the discharge pipe 20.

[0034] Although the present invention has been described with reference to exemplary embodiments, it should be understood that the present invention is not limited to the disclosed exemplary embodiments. The scope of the following claims should be given the broadest interpretation in order to cover all variations and equivalent structures and functions.

Claims

1. A high-pressure homogenizing device for protein purification instruments suitable for viscous samples, comprising a control console (1), a homogenizing cylinder (2), a first slide rod (3), an electric push rod (4), a first fixed seat (5), and an electric stirring rack (6), wherein the first slide rod (3) is slidably mounted on the control console (1), the first fixed seat (5) is rotatably mounted on the first slide rod (3), the electric stirring rack (6) is mounted on the first fixed seat (5), the homogenizing cylinder (2) is clamped on the control console (1), the electric push rod (4) is mounted on the control console (1), the first slide rod (3) is mounted on the telescopic rod of the electric push rod (4), and the control console (1) is electrically connected to the electric stirring rack (6) and the electric push rod (4), characterized in that, It also includes a second fixed seat (7), a second slide rod (8), a first elastic element (9), a mounting plate (10), and a cleaning sponge (11). The second fixed seat (7) is fixedly connected to the first fixed seat (5). The second slide rod (8) is slidably arranged on the second fixed seat (7). The mounting plate (10) is fixedly connected to the second slide rod (8). The cleaning sponge (11) is embedded in the mounting plate (10). The first elastic element (9) is sleeved on the second slide rod (8). The two ends of the first elastic element (9) are respectively connected to the second fixed seat (7) and the second slide rod (8).

2. A high-pressure homogenizer for protein purification instruments suitable for viscous samples as described in claim 1, characterized in that, It also includes a fixed plate (13), a third slide rod (14), a locking block (15), and a second elastic element (16). The fixed plate (13) is fixedly connected to the control panel (1). Two third slide rods (14) are slidably arranged on the fixed plate (13). The locking block (15) is fixedly connected to the third slide rod (14). The second elastic element (16) is sleeved on the third slide rod (14). The two ends of the second elastic element (16) are respectively connected to the fixed plate (13) and the locking block (15). The two locking blocks (15) are respectively locked into the first fixed seat (5) and the second fixed seat (7).

3. A high-pressure homogenizer for protein purification instruments suitable for viscous samples as described in claim 2, characterized in that, It also includes a pressure cylinder (17), a pressure plate (18), a pressure pump (19), and a discharge pipe (20). The pressure pump (19) is installed on the homogenizing cylinder (2), and the pressure cylinder (17) is placed on the control console (1). The pressure cylinder (17) is connected to the pressure pump (19). Five pressure plates (18) are fixedly connected inside the pressure cylinder (17), and the discharge pipe (20) is connected to the pressure cylinder (17).

4. A high-pressure homogenizer for protein purification instruments suitable for viscous samples as described in claim 3, characterized in that, The pressure plate (18) has multiple through holes.

5. A high-pressure homogenizer for protein purification instruments suitable for viscous samples according to claim 4, characterized in that, It also includes a placement plate (12), which is set on the control panel (1).

6. A high-pressure homogenizer for protein purification instruments suitable for viscous samples as described in claim 5, characterized in that, The edges of the card block (15) are rounded.

Images