Purification process of oas1-cas12a rec fusion protein

By optimizing the matching of the encoding gene of the OAS1-Cas12aREC fusion protein with the E. coli translation system and optimizing the purification reagents, the problems of insufficient yield and purity in the existing technology were solved, and efficient and stable purification results were achieved.

CN122128336APending Publication Date: 2026-06-02HAINAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HAINAN UNIV
Filing Date
2026-02-05
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing technologies are insufficient to effectively improve the purification yield and purity of OAS1-Cas12aREC fusion protein, and their stability is inadequate during expression and purification.

Method used

By optimizing the coding gene of the OAS1-Cas12aREC fusion protein to match the translation system of E. coli, the translation bottleneck was eliminated. The composition of reagents in the purification process was also optimized, including the components of bacterial lysis buffer, affinity equilibration buffer and affinity elution buffer. The protein was purified using ultrasonic disruption and affinity chromatography techniques.

Benefits of technology

It improved the yield and purity of the OAS1-Cas12aREC fusion protein, enhanced its stability during expression and purification, prevented protein oxidation and denaturation, and achieved efficient purification results.

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Abstract

This application relates to the field of biotechnology and discloses a purification process for the OAS1-Cas12aREC fusion protein, specifically including its encoding gene, expression vector, and purification process. This application achieves optimal matching between the encoding gene of the OAS1-Cas12aREC fusion protein and the E. coli translation system by targeted optimization, eliminating translational bottlenecks and unfavorable elements, thereby increasing the yield and purity of the OAS1-Cas12aREC fusion protein. Furthermore, it optimizes the reagents used in the protein purification process to further promote the yield and purity of the OAS1-Cas12aREC fusion protein, while also preventing protein oxidation and stabilizing the protein structure, making it less prone to denaturation.
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