Device for rapidly separating and storing livestock and poultry manure bacteria

Abstract

The utility model provides a device for quickly separating and storing livestock and poultry manure bacteria, which relates to the field of biological information extraction and comprises a separation tank, an upper cavity is arranged at the upper end in the separation tank, a lower cavity is arranged at the lower end in the separation tank, and an inlet is arranged on the wall of the upper cavity. According to the utility model, a plurality of links such as sample mixing, primary filtering and secondary filtering are integrated in the separating tank, so that the preparation and storage of bacterial suspension can be completed only by simple operations such as unscrewing a bottle cap, adding a solution and inverting the tank body, and by matching with a magnetic stirrer and gravity filtering; by adopting a two-stage filtering structure consisting of a built-in filter plate and a gauze net, a mixed solution containing bacteria can be rapidly separated, the purity of a suspension can be further improved, the interference of impurities on subsequent experiments such as microorganism culture and DNA extraction is effectively reduced, and the accuracy and reliability of experimental results are guaranteed.

Description

Technical Field

[0001] This utility model relates to the field of bioinformatics extraction, and in particular to a device for the rapid separation and preservation of bacteria in livestock and poultry manure. Background Technology

[0002] In the fields of livestock and poultry health monitoring, disease prevention and control, and gut microbiota research, bacterial community analysis in livestock and poultry manure is an important means of obtaining key information. By isolating and preserving fecal bacteria, researchers can conduct in-depth studies on the structure and function of the gut microbiota, providing a scientific basis for disease diagnosis, feed optimization, and improvement of the breeding environment. However, existing technologies for isolating and preserving livestock and poultry manure bacteria have some shortcomings and cannot meet the needs of efficient and precise research.

[0003] Currently, traditional methods for separating bacteria from feces mainly rely on large-scale equipment such as centrifuges and ultrasonic disruptors. While these devices can achieve bacterial separation, they have significant drawbacks: First, the equipment is expensive, with each centrifuge costing a considerable amount and requiring dedicated laboratory space, limiting its application in grassroots testing institutions and small-scale farms. Second, the operation process is complex, requiring professional technicians for sample pretreatment, parameter adjustment, and equipment maintenance, resulting in lengthy separation operations and severely impacting testing efficiency. Furthermore, traditional methods lack an integrated solution from sample collection to separation and preservation. While some devices have separation capabilities, they lack integrated preservation designs, requiring the transfer of the separated bacterial suspension to other containers for preservation solutions, which can easily lead to bacterial contamination or sample loss. Additionally, the filter components of traditional separation equipment are prone to clogging. Once the filter pores are blocked by fecal particles, the machine must be shut down for disassembly and cleaning, resulting in lengthy maintenance times and poor continuous operating capacity, making it difficult to meet the needs of batch sample processing.

[0004] Therefore, it is necessary to provide a new device for the rapid separation and preservation of bacteria in livestock and poultry manure to solve the above-mentioned technical problems. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a device for rapid separation and preservation of bacteria in livestock and poultry manure.

[0006] The present invention provides a device for rapid separation and preservation of bacteria in livestock and poultry manure, comprising: a separation tank, an upper cavity at the upper end of the separation tank, a lower cavity at the lower end of the separation tank, an inlet on the wall of the upper cavity, an outlet on the wall of the lower cavity, an internal filter plate fixedly connected to the middle of the separation tank, and a bottom cover at the bottom of the separation tank.

[0007] Preferably, a combined filter ring is provided at the outlet position, a gauze mesh is installed and connected in the combined filter ring, the outer ring wall of the combined filter ring is provided with external threads, the inner wall of the outlet port is provided with internal threads, and the combined filter ring is threadedly connected to the inner wall of the outlet.

[0008] Preferably, the inner wall of the bottom cover is provided with an internal thread, and the lower end of the separator is provided with an external thread, and the bottom cover is threadedly connected to the lower end of the separator.

[0009] Preferably, a built-in cross is fixedly connected to the inner wall of the separation tank, a central shaft is rotatably connected to the middle of the built-in cross, and a strip scraper is fixedly connected to the upper end of the central shaft.

[0010] Preferably, the built-in cross has an internal mounting cavity, the lower end of the central shaft is rotatably connected to the central cavity wall of the mounting cavity, a main gear is fixedly connected to the central shaft, a rotating hole is rotatably connected inside the built-in cross, one end of the rotating hole is connected to the mounting cavity, a drive shaft is rotatably connected in the rotating hole, and a secondary gear is installed and connected to one end of the drive shaft, the secondary gear meshing with the main gear.

[0011] Preferably, the main gear and the auxiliary gear mesh with each other, the other end of the drive shaft extends to the outside of the separation tank, and a turntable is installed and connected to the other end of the drive shaft.

[0012] Preferably, a main bottle cap is threadedly connected to the outlet port, and a secondary bottle cap is threadedly connected to the inlet port.

[0013] Compared with related technologies, the device for rapid separation and preservation of bacteria in livestock and poultry manure provided by this utility model has the following beneficial effects:

[0014] 1. This utility model integrates multiple steps such as sample mixing, primary filtration, and secondary filtration into a separation tank. It does not require complicated instruments. Only simple operations such as unscrewing the cap, adding solution, and inverting the tank are needed. With the help of a magnetic stirrer and gravity filtration, bacterial suspension can be prepared and preserved. Operators can quickly get started without professional technical training, which significantly reduces the threshold of experimental operation, saves equipment purchase and training costs, and enables the separation of bacteria from livestock and poultry manure to be carried out efficiently in grassroots laboratories.

[0015] 2. This utility model employs a two-stage filtration structure consisting of a built-in filter plate and a gauze mesh. The pore size of the built-in filter plate can effectively intercept large particles of feces, forming primary filtration. The fine pore size of the gauze mesh at the outlet performs secondary filtration, accurately trapping small impurities. This staged filtration method can quickly separate the mixed liquid containing bacteria and further improve the purity of the suspension. The impurity content of the obtained bacterial suspension is significantly reduced compared to traditional single-stage filtration, effectively reducing the interference of impurities on subsequent experiments such as microbial culture and DNA extraction, and ensuring the accuracy and reliability of experimental results.

[0016] 3. This utility model drives the transmission shaft to rotate by rotating the turntable. The auxiliary gear at one end of the transmission shaft meshes with the main gear on the central shaft, driving the central shaft to rotate, which in turn causes the strip scraper to rotate. This ensures that the filter holes of the built-in filter plate will not become clogged. The clogging problem can be solved without disassembling the device, ensuring that the filtration process is continuous and effectively improving the efficiency of single sample processing. Attached Figure Description

[0017] Figure 1 A schematic diagram of a preferred embodiment of this utility model;

[0018] Figure 2 for Figure 1 The diagram shows the internal structure of the separation tank.

[0019] Figure 3 for Figure 2 The diagram shows the structure of the built-in cross.

[0020] The diagram is labeled as follows: 1. Separator; 2. Upper cavity; 21. Lower cavity; 3. Inlet; 31. Outlet; 4. Internal filter plate; 41. Bottom cover; 5. Combined filter ring; 51. Gauze mesh; 6. Internal cross; 61. Central shaft; 62. Strip scraper; 7. Main gear; 71. Drive shaft; 72. Secondary gear; 8. Turntable; 9. Main cap; 91. Secondary cap. Detailed Implementation

[0021] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0022] Please refer to the following: Figures 1 to 3 A device for rapid separation and preservation of bacteria in livestock and poultry manure includes: a separation tank 1, an upper cavity 2 at the upper end of the separation tank 1, a lower cavity 21 at the lower end of the separation tank 1, an inlet 3 on the wall of the upper cavity 2, an outlet 31 on the wall of the lower cavity 21, an internal filter plate 4 fixedly connected to the middle of the separation tank 1, and a bottom cover 41 at the bottom of the separation tank 1.

[0023] In the specific implementation process, such as Figure 1 and Figure 2 As shown, a combined filter ring 5 is provided at the outlet 31 position, and a gauze mesh 51 is installed and connected in the combined filter ring 5. The outer ring wall of the combined filter ring 5 is provided with external threads, and the inner wall of the outlet 31 is provided with internal threads. The combined filter ring 5 is threadedly connected to the inner wall of the outlet 31.

[0024] It should be noted that: the magnetic stirrer (existing technology) is placed inside the lower cavity 21 beforehand, and then the bottom cap 41 is screwed onto the lower part of the separation tank 1. The auxiliary bottle cap 91 is opened, and the livestock and poultry manure sample is added into the lower cavity 21 through the inlet 3. The corresponding solutions are added sequentially according to a ratio of manure, 1.8 g / L sodium chloride solution, and sterile glycerol preservation solution of 2:1:1. After the addition is complete, with the bottom cap 41 tightly sealed, the separation tank is placed on the magnetic disk (existing technology), and the magnetic stirrer is driven to stir at 1500 rpm / min for 20 minutes to accelerate the dispersion of manure particles and promote the release of bacteria from the manure into the solution.

[0025] The external threads on the outer wall of the combined filter ring 5 and the internal threads on the inner wall of the outlet port 31 form a precise fit, ensuring a tight connection and easy disassembly. The gauze mesh 51 allows the bacterial suspension to pass through smoothly while effectively intercepting impurity particles with a diameter greater than 1mm.

[0026] refer to Figure 1 and Figure 2 As shown, the inner wall of the bottom cover 41 is provided with an internal thread, and the lower end of the separation tank 1 is provided with an external thread. The bottom cover 41 is threadedly connected to the lower end of the separation tank 1.

[0027] It should be noted that the inner wall of the bottom cover 41 and the threaded connection between the inner wall and the lower end of the tank opening of the separator 1 adopt a double-thread design. Compared with a single-thread design, the tightening efficiency is doubled and the sealing performance is better. The detachable bottom cover 41 allows the magnetic stirrer to be conveniently added.

[0028] refer to Figure 2 and Figure 3 As shown, an internal cross 6 is fixedly connected to the inner wall of the separation tank 1, a central shaft 61 is rotatably connected to the middle position of the internal cross 6, and a strip scraper 62 is fixedly connected to the upper end of the central shaft 61.

[0029] It should be noted that the length of the strip scraper 62 is matched with the diameter of the built-in filter plate 4 to ensure that it can fully cover the surface of the filter plate when rotating.

[0030] The built-in filter plate 4 is made of porous stainless steel, which provides high-efficiency filtration while ensuring rigidity and strength.

[0031] refer to Figure 3As shown, the built-in cross 6 has an internal mounting cavity. The lower end of the central shaft 61 is rotatably connected to the central cavity wall of the mounting cavity. A main gear 7 is fixedly connected to the central shaft 61. A rotating hole is rotatably connected inside the built-in cross 6. One end of the rotating hole is connected to the mounting cavity. A drive shaft 71 is rotatably connected in the rotating hole. A secondary gear 72 is installed and connected to one end of the drive shaft 71. The secondary gear 72 meshes with the main gear 7.

[0032] It should be noted that rotating the turntable 8 drives the drive shaft 71 to rotate. The auxiliary gear 72 at one end of the drive shaft 71 meshes with the main gear 7 on the central shaft 61, driving the central shaft 61 to rotate, which in turn causes the strip scraper 62 to rotate, ensuring that the filter holes of the built-in filter plate 4 will not become clogged.

[0033] refer to Figure 1 and Figure 3 As shown, the main gear 7 and the secondary gear 72 mesh with each other, and the other end of the drive shaft 71 extends to the outside of the separation tank 1. A turntable 8 is installed and connected to the other end of the drive shaft 71.

[0034] It should be noted that the turntable 8 is located outside the processing tank 1 for easy operation by staff.

[0035] refer to Figure 1 and Figure 2 As shown, the main bottle cap 9 is threadedly connected to the port of outlet 31, and the auxiliary bottle cap 91 is threadedly connected to the port of inlet 3.

[0036] It should be noted that the main cap 9 and the secondary cap 91 are designed with anti-slip textures on their surfaces to facilitate unscrewing and tightening.

[0037] Open the secondary bottle cap 91, and put the livestock and poultry manure sample into the lower cavity 21 through the inlet 3. Add the corresponding solutions in sequence according to the ratio of manure, 1.8g / L sodium chloride solution and sterile glycerol preservation solution 2:1:1 to complete the addition work.

[0038] Open the main bottle cap 9, and the suspension flows out from the outlet 31, so that the gauze mesh 51 in the combined filter ring 5 at the outlet 31 can directly perform secondary filtration.

[0039] The working principle of the device for rapid separation and preservation of bacteria in livestock and poultry manure provided by this utility model is as follows: A magnetic stirrer (existing technology) is placed inside the lower cavity 21 beforehand, and then the bottom cap 41 is screwed onto the lower part of the separation tank 1. The auxiliary cap 91 is opened, and the livestock and poultry manure sample is added into the lower cavity 21 through the inlet 3. The corresponding solutions are added sequentially according to a ratio of manure, 1.8 g / L sodium chloride solution, and sterile glycerol preservation solution of 2:1:1. After the addition is complete, with the bottom cap 41 tightly sealed, the separation tank is placed on the magnetic disk (existing technology), and the magnetic stirrer is driven to stir at 1500 rpm / min for 20 minutes to accelerate the dispersion of manure particles and promote the release of bacteria from the manure into the solution.

[0040] When the filter holes of the built-in filter plate 4 become clogged, the rotating disc 8 drives the drive shaft 71 to rotate. The auxiliary gear 72 at one end of the drive shaft 71 meshes with the main gear 7 on the central shaft 61, driving the central shaft 61 to rotate. This causes the strip scraper 62 to rotate as well, ensuring that the filter holes of the built-in filter plate 4 will not become clogged, thus ensuring that the primary filtration can proceed normally.

[0041] After the mixing process is completed, the separation tank 1 is inverted. The primary fecal suspension in the lower cavity 21 undergoes preliminary filtration through the built-in filter plate 4 under gravity, effectively intercepting large particles in the feces. The mixed liquid containing bacteria flows through the filter holes into the upper cavity 2, completing the primary filtration and forming a primary fecal suspension. Then, the main bottle cap 9 is opened, and the suspension flows out from the outlet 31. At this time, the gauze mesh 51 in the combined filter ring 5 at the outlet 31 plays its role. Its 1mm×1mm pore size further intercepts residual fine feed residue, particulate impurities, etc., resulting in a purer fecal suspension. The combined filter ring 5 is connected to the outlet 31 by external threads, which facilitates timely disassembly and replacement of the gauze mesh 51 according to usage, ensuring the filtration effect.

[0042] After secondary filtration, the fecal suspension can be directly collected into a sterile container. Due to the addition of sterile glycerol preservative, this suspension can be directly used for subsequent molecular biological analyses such as microbial culture and DNA extraction, or for cryopreservation. The entire device can rapidly obtain fecal bacterial suspensions that meet experimental requirements, significantly improving sample processing efficiency.

[0043] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A device for the rapid separation and preservation of bacteria in livestock and poultry manure, characterized in that, include: The separation tank (1) has an upper cavity (2) at the upper end and a lower cavity (21) at the lower end. The upper cavity (2) has an inlet (3) on its wall and the lower cavity (21) has an outlet (31) on its wall. An internal filter plate (4) is fixedly connected to the middle of the separation tank (1). The bottom of the separation tank (1) has a bottom cover (41).

2. The device for rapid separation and preservation of bacteria in livestock and poultry manure according to claim 1, characterized in that, A combined filter ring (5) is provided at the outlet (31), and a gauze mesh (51) is installed and connected in the combined filter ring (5). The outer ring wall of the combined filter ring (5) is provided with an external thread, and the inner wall of the outlet (31) is provided with an internal thread. The combined filter ring (5) is threadedly connected to the inner wall of the outlet (31).

3. The device for rapid separation and preservation of bacteria in livestock and poultry manure according to claim 1, characterized in that, The inner wall of the bottom cover (41) is provided with an internal thread, and the lower end of the separation tank (1) is provided with an external thread. The bottom cover (41) is threadedly connected to the lower end of the separation tank (1).

4. The device for rapid separation and preservation of bacteria in livestock and poultry manure according to claim 1, characterized in that, An internal cross (6) is fixedly connected to the inner wall of the separation tank (1). A central shaft (61) is rotatably connected to the middle position of the internal cross (6). A strip scraper (62) is fixedly connected to the upper end of the central shaft (61).

5. The apparatus for rapid separation and preservation of bacteria in livestock and poultry manure according to claim 4, characterized in that, The built-in cross (6) has an internal mounting cavity. The lower end of the central shaft (61) is rotatably connected to the central cavity wall of the mounting cavity. A main gear (7) is fixedly connected to the central shaft (61). A rotating hole is rotatably connected inside the built-in cross (6). One end of the rotating hole is connected to the mounting cavity. A drive shaft (71) is rotatably connected in the rotating hole. A secondary gear (72) is installed and connected to one end of the drive shaft (71). The secondary gear (72) meshes with the main gear (7).

6. The apparatus for rapid separation and preservation of bacteria in livestock and poultry manure according to claim 5, characterized in that, The main gear (7) meshes with the secondary gear (72), and the other end of the drive shaft (71) extends to the outside of the separation tank (1). A turntable (8) is installed and connected to the other end of the drive shaft (71).

7. The apparatus for rapid separation and preservation of bacteria in livestock and poultry manure according to claim 1, characterized in that, The outlet (31) is threaded with a main cap (9), and the inlet (3) is threaded with a secondary cap (91).

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