Portable nucleic acid detection device

Abstract

The utility model provides a portable nucleic acid detection device which comprises a shell, a containing space is arranged in the shell, an amplification reaction module, a fluorescence detection module, a nucleic acid extraction module and a control module are assembled in the containing space, the amplification reaction module and the fluorescence detection module are arranged in the lower area of the containing space side by side, and the control module is arranged in the containing space. The nucleic acid extraction module and the control module are both located in the upper area of the containing space. According to the utility model, the total volume of the whole device is effectively reduced, the portability of the device is improved, and the popularization and application of the nucleic acid detection device in an instant field detection scene are facilitated.

Description

Technical Field

[0001] This utility model belongs to the field of nucleic acid testing equipment design technology, specifically relating to a portable nucleic acid testing device. Background Technology

[0002] Nucleic acid testing, as a core technology in the field of molecular diagnostics, has important applications in pathogen identification and gene mutation detection. Current mainstream real-time fluorescence PCR (qPCR) detection equipment relies on complex thermal cycling systems, sophisticated optical modules, and multi-level drive control units. This results in bulky equipment with high power consumption, and stringent requirements for the operating environment and conditions, limiting its widespread application in point-of-care testing scenarios.

[0003] In recent years, LAMP (Loop-Mediated Isothermal Amplification) technology has attracted widespread attention due to its advantages such as rapid reaction and low device dependence; Argonaute proteins have shown great potential in the field of molecular recognition due to their high specificity in nucleic acid recognition. However, the integration of LAMP and Argonaute systems in POCT devices is still in the experimental verification and system optimization stage, facing several engineering challenges such as temperature control coordination, module collaboration, and signal acquisition adaptation, and a mature commercial integrated platform has not yet been formed. Furthermore, existing nucleic acid detection devices adapted to LAMP-Argonaute nucleic acid detection systems have an unreasonable internal component layout, resulting in a large overall size and insufficient portability, which is not conducive to their widespread application in point-of-care testing scenarios. Utility Model Content

[0004] Therefore, the technical problem to be solved by this utility model is to provide a portable nucleic acid testing device to solve the problem that the layout of the internal components of the existing nucleic acid testing device is unreasonable, resulting in an overall large size of the device, which is not portable enough and not conducive to its promotion and application in real-time on-site testing scenarios.

[0005] To address the aforementioned problems, this utility model provides a portable nucleic acid detection device, including a shell with an accommodating space inside. An amplification reaction module, a fluorescence detection module, a nucleic acid extraction module, and a control module are assembled within the accommodating space. The amplification reaction module and the fluorescence detection module are arranged side-by-side in the lower region of the accommodating space, while the nucleic acid extraction module and the control module are both located in the upper region of the accommodating space.

[0006] In some embodiments, the accommodating space is further provided with a heat insulation component, which is located between the amplification reaction module and the fluorescence detection module, and the heat insulation component has a through hole for the detection optical path of the fluorescence detection module to pass through.

[0007] In some embodiments, the housing includes a front shell, a rear shell, and a base, which are detachable and assembled into a single unit to form the sealed accommodating space.

[0008] In some embodiments, the amplification reaction module is located on the side of the fluorescence detection module closer to the front shell, and the front shell has an openable and closable door, the position of which corresponds to the sample loading port of the amplification reaction module.

[0009] In some embodiments, an air outlet is formed on the rear shell that connects the accommodating space with the external environment, and an air inlet is formed on the base that connects the accommodating space with the external environment, and the position of the air inlet corresponds to the position of the temperature control component of the amplification reaction module.

[0010] In some embodiments, the accommodating space is further provided with a temperature-controlled fan, which can drive air from the external environment into the accommodating space through the air inlet and discharge the air from the accommodating space through the air outlet; and / or, a HEPA air filter is provided on the inner side wall of the rear shell at a position corresponding to the air outlet.

[0011] In some embodiments, a control display screen is provided on the upper region of the front housing.

[0012] In some embodiments, the front housing has a tilted plane, and the control display screen is mounted on the tilted plane.

[0013] In some embodiments, the rear cover is provided with a power switch, a USB port, a network interface, and a power interface.

[0014] In some embodiments, the base has multiple anti-slip pads and / or casters with brakes on its bottom side.

[0015] This utility model provides a portable nucleic acid detection device. The heavier and larger amplification reaction module is positioned in the lower region of the accommodating space, while the relatively smaller fluorescence detection module is also positioned in the lower region of the accommodating space. This close proximity minimizes the overall space occupied by the amplification reaction module and fluorescence detection module, thus reducing the lower volume of the outer casing. The heavier amplification reaction module in the lower region also lowers the device's center of gravity, making it more stable. Simultaneously, the nucleic acid extraction module and control module are positioned in the upper region of the accommodating space, facilitating the transfer of liquids from the various accommodating tubes of the nucleic acid extraction chip within the amplification reaction module. The components in the upper region are relatively smaller in mass and volume, which is beneficial for heat dissipation control. This effectively reduces the overall size of the device, improving its portability and making it more suitable for widespread application in real-time on-site testing scenarios. Attached Figure Description

[0016] Figure 1 This is a front view schematic diagram of the portable nucleic acid detection device according to an embodiment of the present invention;

[0017] Figure 2 for Figure 1 Right view of the portable nucleic acid testing device in the image;

[0018] Figure 3 for Figure 1 Rear view of the portable nucleic acid testing device in the image;

[0019] Figure 4 yes Figure 1 A bottom view of a portable nucleic acid testing device in China;

[0020] Figure 5 yes Figure 1 A 3D view of a portable nucleic acid testing device.

[0021] The reference numerals in the attached figures are as follows:

[0022] 1. Outer shell; 11. Front shell; 12. Rear shell; 13. Base; 14. Chamber door; 2. Amplification reaction module; 31. Air outlet; 32. Air inlet; 4. Fluorescence detection module; 5. Nucleic acid extraction module; 6. Heat insulation component; 72. Control display screen; 81. Power switch; 82. USB port; 83. Network interface; 84. Power interface; 9. Anti-slip feet. Detailed Implementation

[0023] See also Figures 1 to 5As shown in the figure, according to an embodiment of the present invention, a portable nucleic acid detection device is provided, including a shell 1. The shell 1 has an accommodating space (not labeled in the figure). An amplification reaction module 2, a fluorescence detection module 4, a nucleic acid extraction module 5, and a control module (not shown in the figure, not labeled) are assembled in the accommodating space. The amplification reaction module 2 and the fluorescence detection module 4 are arranged side by side in the lower region of the accommodating space, while the nucleic acid extraction module 5 and the control module are both located in the upper region of the accommodating space. The aforementioned amplification reaction module 2, fluorescence detection module 4, nucleic acid extraction module 5, and control module can all adopt relevant functional components in the prior art. The present invention does not improve the structure and implementation of the aforementioned components, nor does it intend to protect them. The present invention only optimizes the layout of the aforementioned functional modules in the accommodating space to make more reasonable use of the accommodating space in the shell 1, thereby achieving a compact overall structure of the device, reducing the device size, and improving portability.

[0024] In this technical solution, the heavier and larger amplification reaction module 2 is placed in the lower region of the accommodating space, while the relatively smaller fluorescence detection module 4 is also placed in the lower region of the accommodating space. This close proximity reduces the overall space occupied by the amplification reaction module 2 and the fluorescence detection module 4, thus reducing the lower volume of the outer casing 1. The heavier amplification reaction module 2 being located in the lower region also lowers the center of gravity of the device, making it more stable. Meanwhile, placing the nucleic acid extraction module 5 and the control module in the upper region of the accommodating space facilitates the transfer of liquids from the various accommodating tubes of the nucleic acid extraction chip within the amplification reaction module 2. The components in the upper region are relatively small in weight and volume (the main structure of the nucleic acid extraction module 5 is a liquid suction chamber and a magnetic sleeve structure, which is a slender structure that moves up and down), which is beneficial for heat dissipation of the control components. This effectively reduces the overall volume of the entire device, improves its portability, and facilitates the widespread application of this nucleic acid detection device in real-time on-site testing scenarios.

[0025] Specifically, in a specific embodiment not shown, the aforementioned amplification reaction module 2 is equipped with a constant temperature heating component (e.g., a constant temperature heating plate that can be controlled to rotate) and corresponding components such as a temperature control sensor. In specific applications, the nucleic acid extraction chip will be placed and positioned on the top surface of the aforementioned constant temperature heating component for LAMP isothermal amplification and Argonaute cleavage reaction. The aforementioned fluorescence detection module 4 includes a fluorescence excitation light source, a filter, and a photoelectric collector to detect the fluorescence signal of Argonaute cleavage. The aforementioned nucleic acid extraction module 5 is used for sample lysis, magnetic bead adsorption, and elution, and integrates a magnetic rod assembly, a blow-and-suction device, and a sample loading module. The aforementioned modules can be processed in an orderly manner according to the known LAMP-Argonaute nucleic acid detection process under the control instructions of the control module. In addition, the aforementioned control module includes a corresponding main control motherboard and a corresponding power supply module, etc. As these are conventional technologies in the field, they will not be described in detail here.

[0026] In some embodiments, a heat insulation component 6 is further provided in the accommodating space. The heat insulation component 6 is located between the amplification reaction module 2 and the fluorescence detection module 4, and a through hole is formed on the heat insulation component 6 to allow the detection optical path of the fluorescence detection module to pass through.

[0027] In this technical solution, the heat insulation component 6 is disposed between the amplification reaction module 2 and the fluorescence detection module 4, which can effectively prevent the heat of the amplification reaction module 2 during operation from being conducted to the fluorescence detection module 4, thereby improving the detection accuracy of the fluorescence detection module 4.

[0028] In one specific embodiment, the outer shell 1 includes a front shell 11, a rear shell 12, and a base 13. The front shell 11, the rear shell 12, and the base 13 can be detachably assembled into a single unit to form the sealed accommodating space. Specifically, the aforementioned front shell 11 is an integrated cover structure with a front wall, a top wall, and two side walls, while the main body of the aforementioned rear shell 12 and the base 13 is a flat plate structure. Thus, the three are detachably connected into a single unit by snaps or screws, which facilitates the assembly of the aforementioned functional modules within the accommodating space.

[0029] In some embodiments, the amplification reaction module 2 is located on the side of the fluorescence detection module 4 near the front shell 11, which facilitates the placement of the nucleic acid extraction chip inside the amplification reaction module 2. The front shell 11 has an openable and closable door 14, the position of which corresponds to the sample loading port of the amplification reaction module 2. In a specific embodiment, one side of the door 14 is hinged to the front shell 11, while the other side of the door 14 is detachably connected to the front shell 11 through a snap-fit ​​structure, so as to keep the aforementioned accommodating space relatively sealed during nucleic acid extraction, amplification and detection, and to form a certain degree of isolation from the external environment.

[0030] In some embodiments, the rear shell 12 has an air outlet 31 that connects the accommodating space with the external environment, and the base 13 has an air inlet 32 ​​that connects the accommodating space with the external environment. The position of the air inlet 32 ​​corresponds to the position of the temperature control component (including the aforementioned constant temperature heating component) of the amplification reaction module 2. Furthermore, the accommodating space is also provided with a temperature control fan (not shown in the figure, not labeled), which can drive air from the external environment into the accommodating space through the air inlet 32 ​​and exhaust it from the accommodating space through the air outlet 31.

[0031] In this technical solution, an air inlet 32 ​​corresponding to the position of the temperature control component is set on the base 13, so that the external airflow can enter the housing space under the drive of the temperature control fan to dissipate heat from the various components inside, especially the temperature control component, and ensure that the temperature control component can be reliably and accurately adjusted.

[0032] In some embodiments, a HEPA air filter is provided on the inner sidewall of the rear housing 12 at a position corresponding to the air outlet 31, so as to filter the airflow discharged from the containment space and prevent aerosols from spreading into the external environment and causing environmental pollution and infection risks.

[0033] See details Figure 1 As shown, in some embodiments, a control display screen 72 is provided on the upper part of the front shell 11. The user (operator) can control the operation of the nucleic acid detection device by operating the control display screen 72. It can also serve as an interface for adjusting nucleic acid detection parameters and displaying detection results. This utility model does not particularly limit the specific implementation of the control display screen 72. In principle, it can adopt relevant technical solutions in the prior art to achieve its functional requirements. This utility model sets the control display screen 72 in the upper part of the front shell 11, which can facilitate the operation of the user and the observation of relevant parameters, and has better user comfort.

[0034] In one specific embodiment, the front shell 11 has an inclined plane, and the control display screen 72 is assembled on the inclined plane, which can further improve the user's ease of operation of the control display screen 72. In one specific embodiment, the tilt angle of the aforementioned inclined plane is about 65° (the tilt angle with the horizontal plane).

[0035] In some embodiments, the rear cover 12 is provided with a power switch 81, a USB port 82, a network interface 83, and a power interface 84. In this technical solution, the power switch 81, USB port 82, network interface 83, and power interface 84 are all located on the rear cover 12, that is, on the back of the detection device, which can ensure the maximum area design of the aforementioned control display screen 72 and higher space utilization.

[0036] In some embodiments, the bottom side of the base 13 is provided with multiple anti-slip pads 9 and / or casters with brakes (not shown in the figure), which facilitates the rapid transport and deployment of the detection device and further improves the portability of the detection device.

[0037] In one specific embodiment, the outer dimensions of the aforementioned housing 1 are length * width * height = 229 * 128 * 311 (unit: mm), which is suitable for portable applications.

[0038] It will be readily understood by those skilled in the art that the aforementioned advantageous methods can be freely combined and superimposed without conflict.

[0039] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model. The above are only preferred embodiments of this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of this utility model, and these improvements and modifications should also be considered within the protection scope of this utility model.

Claims

1. A portable nucleic acid detection device, characterized by, The application relates to a nucleic acid amplification and detection device, which comprises a shell (1) with a containing space in the shell (1), an amplification reaction module (2), a fluorescence detection module (4), a nucleic acid extraction module (5) and a control module assembled in the containing space, wherein the amplification reaction module (2) and the fluorescence detection module (4) are arranged side by side in a lower region of the containing space, and the nucleic acid extraction module (5) and the control module are both arranged in an upper region of the containing space.

2. The portable nucleic acid detection device according to claim 1, wherein The containing space is further provided with a heat insulation piece (6) between the amplification reaction module (2) and the fluorescence detection module (4), and the heat insulation piece (6) is provided with a through hole for the detection light path of the fluorescence detection module.

3. The portable nucleic acid detection device of claim 1, wherein The shell (1) comprises a front shell (11), a rear shell (12) and a base (13), and the front shell (11), the rear shell (12) and the base (13) are detachably assembled into an integrated whole to form a closed containing space.

4. The portable nucleic acid detection device according to claim 3, wherein The amplification reaction module (2) is arranged on a side of the fluorescence detection module (4) close to the front shell (11), the front shell (11) is provided with an openable and closable door (14), and the position of the door (14) corresponds to the position of a sample loading port of the amplification reaction module (2).

5. The portable nucleic acid detection device according to claim 3, wherein The rear shell (12) is provided with an air outlet (31) penetrating through the containing space and the external environment, the base (13) is provided with an air inlet (32) penetrating through the containing space and the external environment, and the position of the air inlet (32) corresponds to the position of a temperature control component of the amplification reaction module (2).

6. The portable nucleic acid detection device according to claim 5, wherein The containing space is further provided with a temperature control fan, which can drive air in the external environment into the containing space through the air inlet (32) and discharge the air out of the containing space through the air outlet (31). The inner side wall of the rear shell (12) is provided with a HEPA air filter at a position corresponding to the air outlet (31).

7. The portable nucleic acid detection device of claim 3, wherein A control display screen (72) is arranged on an upper region of the front shell (11).

8. The portable nucleic acid detection device of claim 7, wherein, The front shell (11) has an inclined plane, and the control display screen (72) is assembled on the inclined plane.

9. The portable nucleic acid detection device of claim 3, wherein The rear shell (12) is provided with a power switch (81), a USB socket (82), a network interface (83) and a power supply interface (84).

10. The portable nucleic acid detection device of claim 3, wherein The bottom side of the base (13) is provided with a plurality of anti-skid foot pads (9) and / or brake universal wheels.

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