Cell resuscitation instrument capable of realizing accurate temperature control

Abstract

The utility model discloses a cell resuscitation instrument capable of realizing accurate temperature control, which comprises a shell, a plurality of heating modules for cell resuscitation are arranged in the upper space of the shell at intervals, and each heating module comprises a heating groove for placing a cryopreservation tube. The temperature detection module is used for detecting temperature; the main control unit is used for triggering the heating module to heat the cryopreservation tube; the main control unit is electrically connected with the temperature detection module to realize timing control and temperature control of cell resuscitation; and a waste heat isolation device for blocking a heat source of the heated cryopreservation pipe is arranged in the heating tank. According to the structure, temperature control protection is achieved through the temperature detection module and the main control unit, accurate temperature control is achieved under the cooperation of the waste heat isolation device, multiple samples can be treated at the same time through the multiple heating modules arranged at intervals, the high-throughput experiment requirement is met, operation is easy in the whole cell resuscitation process, and sample pollution is avoided.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and in particular to a cell resuscitation device that can achieve precise temperature control. Background Technology

[0002] Cell resuscitation is a crucial step in cell culture, aiming to restore cryopreserved cells to a physiological state for subsequent experiments or applications. Traditional cell resuscitation methods typically employ water baths. However, due to water's high heat capacity, this method presents several inconveniences, including slow heating and cooling rates, imprecise temperature control, and high energy consumption for heating. Furthermore, the water bath method is cumbersome and prone to contamination of the cryopreserved cell samples due to improper handling. To address these issues, hot air heating has been employed. However, hot air heating suffers from low heat transfer efficiency due to air's poor thermal conductivity, resulting in slow heating rates and high energy consumption. Additionally, the equipment is bulky, and hot air heating can generate noise pollution. Both traditional water baths and existing hot air heating methods struggle to process multiple samples simultaneously, leading to low experimental efficiency and failing to meet the demands of high-throughput experiments. Utility Model Content

[0003] To solve the above-mentioned technical problems, this utility model provides a cell resuscitation device that can achieve precise temperature control.

[0004] The technical solution of this utility model is as follows: it includes a shell, and the upper space of the shell is provided with a plurality of heating modules for cell resuscitation. The heating module includes a heating tank for placing cryovials, a temperature detection module for detecting temperature, and a main control unit for triggering the heating module to heat the cryovials. The main control unit is electrically connected to the temperature detection module to realize timed control and temperature control of cell resuscitation. The heating tank is provided with a residual heat isolation device to block the heat source of the heated cryovials.

[0005] Preferably, the waste heat isolation device adopts an ejection detachment structure to eject the cryopreservation tube from the heating tank.

[0006] Preferably, the ejection and release structure includes a top pin and a locking device. The top pin is driven to move by the elastic deformation of the elastic element, and the bottom of the top pin passes through the bottom of the heating tank and is connected to the locking device.

[0007] Preferably, the locking device includes a magnetic snap connected to the bottom of the top pin and an electromagnet spaced below the magnetic snap. When the magnetic snap moves down to close with the electromagnet, the main control unit controls the electromagnet to be energized and generate electromagnetic force. The electromagnet and the magnetic snap are stably attracted to each other under the action of electromagnetic force.

[0008] Preferably, a positioning sensor for detecting the position of the magnetic clasp is provided on one side of the electromagnet, and the positioning sensor is electrically connected to the main control unit.

[0009] Preferably, the temperature detection module uses temperature sensors, and several temperature sensors are embedded on the periphery of the heating tank, and all of the temperature sensors are electrically connected to the main control unit.

[0010] Preferably, the mounting bracket includes an upper mounting plate with a plurality of mounting holes, and the lower end of the heating groove has a mounting boss extending vertically downward, the outer side wall of the mounting boss being tightly fitted with the inner side wall of the mounting hole.

[0011] Preferably, the heating module includes a heating element and a heat conduction block disposed above the heating element, and the heating groove is a cavity structure formed by processing the heat conduction block.

[0012] Preferably, the heat conduction block is made of aluminum material with a high thermal conductivity.

[0013] Preferably, the top pin is made of a heat-resistant material, and the elastic element is located between the top boss of the top pin and the bottom of the heating groove.

[0014] The beneficial technical effects of this utility model are:

[0015] This structure features several resuscitation devices spaced apart on a mounting frame. It incorporates multiple independent heating modules for each sample, with individual temperature and time control for each module. Real-time monitoring of the temperature and resuscitation status of each sample is achieved through a main control unit and sensors embedded in the heating tank. Furthermore, each heating module is equipped with a residual heat isolation device to automatically eject the heated cryovials, thereby blocking and reducing residual heat transfer and preventing inaccurate temperature control due to residual heat effects. Simultaneously, this structure enables simultaneous processing of multiple samples to meet the high-throughput sample resuscitation needs of modern biological experiments.

[0016] Moreover, the cell resuscitation process is carried out in a sealed heating tank, which effectively avoids the pollution problems associated with the traditional water bath method. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall appearance of this utility model;

[0018] Figure 2 This is a schematic diagram of the internal structure of the casing of this utility model;

[0019] Figure 3 This is a cross-sectional schematic diagram of the specific structure of the resuscitation device of this utility model;

[0020] Figure 4 This is a schematic diagram of the relevant structure of the mounting bracket of this utility model;

[0021] The components include: 1. Shell; 11. Outer shell; 12. Top cover; 121. Test tube hole; 2. Resuscitation device; 21. Heating module; 211. Heating element; 212. Heat conduction block; 22. Heating tank; 23. Temperature sensor; 3. Residual heat isolation device; 31. Top pin; 32. Magnetic buckle; 33. Electromagnet; 34. Positioning sensor; 4. Mounting bracket; 41. Upper mounting plate; 411. Mounting hole; 42. Second mounting plate; 421. Airflow hole; 43. Lower mounting plate; 5. Heat dissipation device; 6. Control panel. Detailed Implementation

[0022] In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0023] like Figures 1 to 3 As shown, the present invention provides a cell resuscitation device capable of precise temperature control, comprising a housing 1, which encloses an upper space and a lower space. The upper space is provided with a plurality of resuscitation devices 2 arranged at intervals. The resuscitation devices 2 are controlled by a main control unit for timing and temperature control, and include a heating module 21 and a heating tank 22 through which heat is conducted. Cell cryopreservation containers can be placed in the heating tank 22 to complete cell resuscitation.

[0024] The heating module includes a heating element 211 and a heat conduction block 212 disposed above the heating element 211. The heating groove 22 is a cavity structure formed by processing the heat conduction block 212. The heat conduction block 212 is made of a high thermal conductivity material, which can quickly and evenly transfer the heat generated by the heating element 211 to the heating groove 22, ensuring that the cells in the cell cryopreservation container are thawed at a suitable temperature.

[0025] In this embodiment, the heat conduction block 212 is made of aluminum.

[0026] The heating element 211 is electrically connected to the main control unit, and the bottom of the heat conduction block 212 matches the heating element 211.

[0027] The temperature detection module uses temperature sensors 23. Several temperature sensors 23 are embedded on the periphery of the heating tank 22 to monitor the temperature uniformity in the heating tank 22 in real time. Several temperature sensors 23 are electrically connected to the main control unit.

[0028] In this embodiment, the cell cryopreservation container is a cryopreservation tube, and the heating tank 22 is a cylindrical cavity. The outer wall of the cryopreservation tube is in contact with the inner wall of the heating tank 22 to achieve effective heat conduction between the heating tank 22 and the cryopreservation tube.

[0029] Furthermore, to better coordinate with the main control unit to achieve timed and temperature control of the cell cryopreservation container and avoid temperature overshoot caused by residual heat, thereby affecting the accuracy of temperature control, in this embodiment, a residual heat isolation device 3 is provided inside the heating tank 22. The temperature sensor 23 monitors the temperature of the heating tank 22 in real time and transmits the signal to the main control unit. When the cell cryopreservation container is heated to the set temperature, the main control unit cuts off the heating power supply through the control circuit and simultaneously triggers the residual heat isolation device 3 to block the cryopreservation tube from the heat source.

[0030] In this embodiment, the waste heat isolation device 3 adopts an ejection and release structure to eject the cryopreservation tube from the heating tank 22. The waste heat isolation device 3 includes a top pin 31 and a locking device. The top pin 31 is driven to move by the elastic deformation of the elastic element. The bottom of the top pin passes through the bottom of the heating tank and is connected to the locking device.

[0031] In this embodiment, the elastic element is a spring, which stores and releases energy through elastic deformation, and drives the top pin 31 to move when releasing energy. The spring is sleeved on the upper part of the top pin 31, and the spring is located between the top boss of the top pin 31 and the bottom of the heating groove 22.

[0032] The top pin 31 is made of heat-resistant material to effectively block the transfer of residual heat to the cell cryopreservation container and ensure precise temperature control.

[0033] The locking device includes a magnetic snap 32 connected to the bottom of the top pin 31 and an electromagnet 33 spaced below the magnetic snap 32. In use, the cryopreservation tube is placed in the heating tank 22 and downward pressure is applied to move the magnetic snap 32 downwards until it closes with the electromagnet 33. At this time, the elastic element is in a compressed state. The main control unit controls the electromagnet 33 to be energized and generate electromagnetic force. The magnetic snap 32 and electromagnet 33 are firmly attracted under the action of electromagnetic force, ensuring that the top pin 31 is locked. At the same time, the heating element 211 continuously heats the cryopreservation tube through the heating tank 22. When the internal temperature of the heating tank 22 reaches the preset value and the set working time is completed, the main control unit immediately cuts off the power to the electromagnet 33, and the electromagnetic force disappears. When the spring resets under the action of elastic potential energy, it drives the top pin 31 to move, pushing the heated cryopreservation tube out of the heating tank 22 so that the operator can remove it in time. This blocks and reduces the transfer of residual heat, avoiding the problem of inaccurate temperature control caused by residual heat effect.

[0034] Furthermore, a positioning sensor 34 is provided on one side of the electromagnet 33 to detect whether the magnetic snap fastener 32 has reached a specific position and to provide timely feedback to the main control unit. In this embodiment, the positioning sensor 34 is a slotted switch. When the magnetic snap fastener 32 moves down and closes with the electromagnet 33, one end of the magnetic snap fastener 32 is inserted into the slotted switch. The main control unit controls the electromagnet 33 to be energized in a timely manner through the received electrical signal from the slotted switch.

[0035] In this embodiment, a mounting bracket 4 is provided inside the housing 1. The mounting bracket 4 includes an upper mounting plate 41, a second mounting plate 42 and a lower mounting plate 43 from top to bottom.

[0036] Several of the aforementioned resuscitation devices 2 are arranged in a matrix on an upper mounting plate 41, which has several mounting holes 411 for the top pins 31 to pass through, such as... Figure 4 To ensure the stable and precise positioning of the heating tank 22, a vertically downward extending mounting boss is provided at the lower end of the heating tank 22. The mounting boss is cylindrical and integrally formed with the heating tank 22. After the mounting boss is installed through the mounting hole 411, the outer wall of the mounting boss fits tightly with the inner wall of the mounting hole 411, thereby providing a solid and reliable mechanical support for the heating tank 22 to prevent the heating tank 22 from shaking or shifting during operation.

[0037] Several electromagnets 33 and slotted switches are disposed on the second mounting plate 42.

[0038] The lower space is equipped with a heat dissipation device 5, which pops out after the cryopreservation tube has finished heating. The heat dissipation device 5 is used to dissipate the residual heat inside the housing 1 in a timely manner to prevent excessive heat accumulation and ensure the stable operation of the internal components of the instrument and the continuous optimization of overall performance. In this embodiment, the heat dissipation device 5 is a cooling fan, which is mounted on the lower mounting plate 43. The second mounting plate 42 is provided with airflow holes 421 for heat circulation to cooperate with the cooling fan to dissipate heat.

[0039] The housing 1 includes an outer shell 11 and an upper cover 12. The outer shell 11 has a ventilation opening on the side corresponding to the cooling fan. The upper cover 12 has a plurality of test tube holes 121 that match each heating tank 22 respectively. The cryopreservation tube passes through the test tube holes 121 and is placed in the corresponding heating tank 22.

[0040] The outer casing 11 is also equipped with a control panel 6, which is electrically connected to the main control unit. The cell resuscitation parameters of each cryopreservation tube can be understood and set through the control panel 6.

[0041] In use, the cryopreservation tubes are placed in the heating tank 22. The recovery time and temperature are set via the control panel 6. The main control unit controls the heating element 211 to heat the heating tank 22. The temperature sensor 23 monitors the temperature inside the heating tank 22 in real time and feeds the temperature signal back to the main control unit. The main control unit adjusts the power of the heating element 211 according to the set temperature to maintain the temperature inside the heating tank 22 at the set value. When the set recovery time is reached, the main control unit triggers a stop heating operation. At the same time, the residual heat isolation device 3 activates, i.e., the spring pushes the top pin 31 to push the cryopreservation tubes out of the heating tank 22 so that the operator can retrieve them in time, while blocking the heat source to ensure the accuracy of the heating process.

[0042] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A cell thawing device capable of precise temperature control, characterized in that: The device includes a housing (1), and the upper space of the housing (1) is provided with several heating modules (21) for cell resuscitation. The heating module (21) includes a heating tank (22) for placing cryopreservation tubes, a temperature detection module for detecting temperature, and a main control unit for triggering the heating module (21) to heat the cryopreservation tubes. The main control unit is electrically connected to the temperature detection module to realize the timing control and temperature control of cell resuscitation. The heating tank (22) is provided with a residual heat isolation device (3) to block the heat source of the cryopreservation tubes after heating.

2. The cell recovery instrument capable of precise temperature control according to claim 1, wherein: The waste heat isolation device (3) adopts an ejection structure to eject the cryopreservation tube from the heating tank (22).

3. The cell resuscitator capable of precise temperature control according to claim 2, wherein: The ejection and release structure includes a top pin (31) and a locking device. The top pin (31) is driven to move by the elastic deformation of the elastic element. The bottom of the top pin (31) passes through the bottom of the heating groove (22) and is connected to the locking device.

4. The cell resuscitator capable of precise temperature control according to claim 3, wherein: The locking device includes a magnetic snap (32) connected to the bottom of the top pin (31) and an electromagnet (33) spaced below the magnetic snap (32). When the magnetic snap (32) moves down to close with the electromagnet (33), the main control unit controls the electromagnet (33) to be energized and generate electromagnetic force. The electromagnet (33) and the magnetic snap (32) are stably attracted to each other under the action of electromagnetic force.

5. The cell resuscitator capable of precise temperature control according to claim 4, wherein: The electromagnet (33) is provided with a positioning sensor (34) on one side for detecting the position of the magnetic clasp (32), and the positioning sensor (34) is electrically connected to the main control unit.

6. The cell resuscitator capable of precise temperature control according to claim 1, wherein: The temperature detection module uses a temperature sensor (23), and several temperature sensors (23) are embedded on the periphery of the heating tank (22), and several of the temperature sensors (23) are electrically connected to the main control unit.

7. The cell resuscitator capable of precise temperature control according to claim 6, wherein: The housing (1) is provided with a mounting bracket (4), which includes an upper mounting plate (41). The upper mounting plate (41) is provided with a plurality of mounting holes (411). The lower end of the heating groove (22) is provided with a mounting boss extending vertically downward. The outer side wall of the mounting boss is in close contact with the inner side wall of the mounting hole (411). 8.The cell resuscitator capable of precise temperature control according to claim 1, wherein: The heating module (21) includes a heating element (211) and a heat conduction block (212) disposed above the heating element (211). The heating groove (22) is a cavity structure formed by processing the heat conduction block (212).

9. The cell resuscitator capable of precise temperature control according to claim 8, wherein: The heat conduction block (212) is made of aluminum material with high thermal conductivity.

10. The cell resuscitator capable of precise temperature control according to claim 3, wherein: The top pin (31) is made of heat-resistant material, and the elastic element is located between the top boss of the top pin (31) and the bottom of the heating groove (22).

Images