A nucleic acid extraction reagent addition device

Abstract

This utility model relates to the field of medical device technology and discloses a nucleic acid extraction reagent addition device, including a support plate. Movable components are fixedly installed on both sides of the top of the support plate, and a fixed bracket is fixedly installed on the top of the support plate. Compared with traditional devices, this device, on the one hand, uses a mechanical structure to move the sample tube below the addition site, achieving precise positioning and reducing human error. On the other hand, it uses a pressure sensing device to determine whether the addition tube is accurately inserted. When the pressure is abnormal, it stops operation in time to avoid collision damage caused by inaccurate insertion, protecting the sample tube and the addition tube, reducing equipment costs. Simultaneously, accurate insertion of the addition tube ensures smooth reagent delivery and addition, guaranteeing the accuracy and stability of reagent addition, improving addition efficiency, and effectively avoiding sample tube crushing damage caused by misalignment, thus providing a guarantee for the smooth conduct of nucleic acid extraction experiments.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and more specifically, to a nucleic acid extraction reagent addition device. Background Technology

[0002] With the rapid development of life sciences, medical diagnostics and other fields, nucleic acid testing has become increasingly widespread. Nucleic acid extraction, as a key preliminary step, is of paramount importance. Traditional manual addition of nucleic acid extraction reagents is not only inefficient but also highly susceptible to human error. Different operators have different techniques, resulting in large deviations in reagent addition and poor experimental repeatability. In the current context of ever-increasing demand for high-throughput, projects such as large-scale infectious disease screening and gene sequencing require the processing of massive amounts of samples, which is difficult to handle manually. In addition, the requirements for detection accuracy are becoming increasingly stringent, and even small errors in reagent addition can mislead diagnostic results. Therefore, nucleic acid extraction reagent addition devices have emerged.

[0003] In the nucleic acid extraction experimental process, the existing nucleic acid extraction reagent addition device has revealed obvious drawbacks. Due to insufficient structural design or positioning accuracy, the precise alignment of the addition tube and the sample tube becomes a major problem in actual operation. Operators face situations where the addition tube cannot be accurately aligned with the sample tube, which can easily lead to collisions between the two. Once a collision occurs, the sample tube will break, and the addition tube will also be damaged. Frequent replacement of broken tubes and damaged addition tubes not only causes the equipment maintenance cost to skyrocket, but also the cleaning and recalibration work after each collision greatly wastes time and seriously affects the overall efficiency of reagent addition. Therefore, improvement and optimization are needed. Utility Model Content

[0004] To overcome the shortcomings of existing technologies, this invention provides a nucleic acid extraction reagent addition device, which has the advantage of precise reagent addition.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a nucleic acid extraction reagent addition device, comprising a support plate, movable components fixedly installed on both sides of the top of the support plate, a fixed bracket fixedly installed on the top of the support plate, a reagent addition component fixedly installed inside the fixed bracket, the reagent addition component including an electric telescopic rod fixedly installed inside the fixed bracket with its output end penetrating the fixed bracket, a pressure plate fixedly installed at the output end of the electric telescopic rod, guide rods fixedly installed around the bottom of the pressure plate, a first pressure sensor fixedly installed at the end of the guide rod, an installation plate fixedly installed on the outer surface of the guide rod with both ends of the guide rod penetrating inside the installation plate, a storage box fixedly installed on the top of the installation plate, an addition tube fixedly installed on the bottom side of the installation plate with the addition tube penetrating the installation plate and communicating with the storage box, a reaction container placement box fixedly installed on the top of the movable components, guide openings corresponding to the guide rods on the outer surface of the reaction container placement box, a second pressure sensor fixedly installed inside the guide openings, and a test tube groove corresponding to the addition tube on the outer surface of the reaction container placement box.

[0006] As a preferred embodiment of this utility model, the movable component includes connecting plates fixedly installed on both sides of the top of the support plate. A rack is fixedly installed on the top of the connecting plate. A groove is formed on the outer surface of the connecting plate. A placement plate is movably installed on the inner side of the two racks. A connecting rod is fixedly installed around the bottom side of the placement plate. A gear is rotatably installed on the outer surface of the connecting rod, and the gear meshes with the rack. A ball bearing is rolled at the bottom end of the connecting rod.

[0007] As a preferred embodiment of this utility model, a flow guide groove is fixedly installed on the inner wall of the adding pipe, a flow limiting block is fixedly installed on the inner side of the flow guide groove, and the bottom of the adding pipe is conical.

[0008] As a preferred technical solution of this utility model, the fixed bracket has rectangular sliding grooves on both sides inside, and extension blocks are fixedly installed on both sides of the lower pressure plate, with the extension blocks extending to the inside of the rectangular sliding grooves.

[0009] As a preferred embodiment of this utility model, a flexible buffer block is fixedly installed on the top of the extension block, and the flexible buffer block is located inside the rectangular groove.

[0010] As a preferred embodiment of this utility model, a flexible pad is fixedly installed on the inner side of the test tube groove, and the flexible pad is in the shape of a ring.

[0011] As a preferred embodiment of this utility model, a flexible buffer rod is fixedly installed on the side of the placement plate, and a baffle is fixedly installed on the top of the bearing plate and located on one side of one end of the two racks.

[0012] As a preferred embodiment of this utility model, a base is fixedly installed at the bottom of the support plate, and the base is fixedly installed around the bottom of the support plate.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0014] 1. This utility model, when the guide rod is accurately inserted into the inner side of the guide port, will cause the first pressure sensor to contact the second pressure sensor, thereby enabling the external delivery mechanism to deliver the reagents required for nucleic acid extraction into the storage box. Simultaneously, the adding tube is aligned with the test tube slot, and then the reagent is added to the inside of the sample test tube through the adding tube. Compared to traditional devices, this device, on the one hand, uses a mechanical structure to move the sample test tube below the adding position, achieving precise positioning and reducing human error; on the other hand, it uses pressure sensors to determine whether the adding tube is accurately inserted, stopping operation promptly when the pressure is abnormal to avoid collision damage caused by inaccurate insertion, protecting the sample test tube and the adding tube, reducing equipment costs. Furthermore, accurate insertion of the adding tube ensures smooth reagent delivery and addition, guaranteeing the accuracy and stability of reagent addition, improving addition efficiency, and effectively avoiding sample test tube damage caused by misalignment, thus providing a guarantee for the smooth conduct of nucleic acid extraction experiments.

[0015] 2. This utility model allows the bottom connecting rod to move when the staff pulls the placement plate, which in turn causes multiple gears to move in a specific direction. At the same time, the ball bearings roll in the sliding groove, which together facilitates the convenient movement of the reaction vessel placement box and provides a wide field of vision for the test tube slots. Compared with traditional devices, this device greatly facilitates the placement of sample test tubes, reduces operational obstacles, and improves the efficiency of preliminary preparation. Furthermore, the four connecting rods, gears, and ball bearings work together to form a stable support, which greatly increases the stability during the movement, reduces the risk of sample damage caused by shaking, and ensures the smooth conduct of the experiment. Attached Figure Description

[0016] Figure 1 This is a frontal three-dimensional appearance structural diagram of the present utility model;

[0017] Figure 2 This is a schematic diagram of the fixed bracket structure of this utility model;

[0018] Figure 3 This is a schematic cross-sectional view of the present invention.

[0019] Figure 4 A schematic diagram of the reagent addition component structure of this utility model;

[0020] Figure 5 This utility model Figure 4 Enlarged view of point A in the middle;

[0021] Figure 6 This is a schematic diagram of the flexible buffer block structure of this utility model;

[0022] Figure 7 This is a schematic diagram of the slide groove structure of this utility model;

[0023] Figure 8 This is a schematic diagram of the reaction vessel placement box structure of this utility model.

[0024] In the diagram: 1. Support plate; 2. Fixed bracket; 3. Reagent adding assembly; 301. Electric telescopic rod; 302. Lower pressure plate; 303. Guide rod; 304. Mounting plate; 305. First pressure sensor; 306. Storage box; 307. Addition tube; 308. Flow guide groove; 309. Flow limiting block; 310. Reaction vessel placement box; 311. Test tube trough; 312. Guide port; 313. Second pressure sensor; 4. Moving assembly; 401. Rack; 402. Connecting plate; 403. Slide groove; 404. Connecting rod; 405. Ball bearing; 406. Gear; 407. Placement plate; 5. Flexible buffer rod; 6. Baffle; 7. Extension block; 8. Flexible buffer block; 9. Rectangular slide groove; 10. Flexible pad; 11. Base. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] like Figures 1 to 8As shown, this utility model provides a nucleic acid extraction reagent adding device, including a support plate 1. Movable components 4 are fixedly installed on both sides of the top of the support plate 1. A fixed bracket 2 is fixedly installed on the top of the support plate 1. A reagent adding component 3 is fixedly installed inside the fixed bracket 2. The reagent adding component 3 includes an electric telescopic rod 301 fixedly installed inside the fixed bracket 2, with the output end of the electric telescopic rod 301 penetrating through the fixed bracket 2. A lower pressure plate 302 is fixedly installed at the output end of the electric telescopic rod 301. Guide rods 303 are fixedly installed around the bottom of the lower pressure plate 302. A first pressure sensor 305 is fixedly installed at the end of the guide rod 303. The guide rod 303 has a... A mounting plate 304 is fixedly mounted on the surface, and both ends of the guide rod 303 pass through the interior of the mounting plate 304. A storage box 306 is fixedly mounted on the top of the mounting plate 304. An addition tube 307 is fixedly mounted on the bottom side of the mounting plate 304 and passes through the mounting plate 304 and communicates with the storage box 306. A reaction container placement box 310 is fixedly mounted on the top of the moving component 4. Guide openings 312 are opened around the outer surface of the reaction container placement box 310 and correspond to the guide rod 303. A second pressure sensor 313 is fixedly mounted inside the guide openings 312. A test tube groove 311 is opened on the outer surface of the reaction container placement box 310 and corresponds to the addition tube 307.

[0027] When the staff places the sample tubes one by one inside the test tube slot 311, and then moves the reaction vessel placement box 310 and the test tube slot 311 to below the fixed bracket 2 via the moving component 4, the electric telescopic rod 301 is activated via the external controller. When the electric telescopic rod 301 is activated, the output shaft will drive the lower pressure plate 302 to gradually move downwards. Then, the lower pressure plate 302 will drive the guide rod 303 to gradually move downwards. At the same time, the guide rod 303 will drive the mounting plate 304 to move downwards together. When the first pressure sensor 305 receives a pressure value greater than the set value, it will... The electric telescopic rod 301 stops operating. Since the pressure value is greater than the set value, it means that the guide rod 303 is not accurately inserted into the guide port 312. At the same time, the adding tube 307 is not aligned with the test tube slot 311. When the guide rod 303 is accurately inserted into the inside of the guide port 312, the first pressure sensor 305 will contact the second pressure sensor 313, thereby allowing the external delivery mechanism to deliver the reagents required for nucleic acid extraction into the storage box 306. At the same time, the adding tube 307 is now aligned with the test tube slot 311, and then the reagent is added to the inside of the sample test tube through the adding tube 307.

[0028] When the guide rod 303 is accurately inserted into the guide port 312, the first pressure sensor 305 will contact the second pressure sensor 313, thereby enabling the external delivery mechanism to deliver the reagents required for nucleic acid extraction into the storage tank 306. Simultaneously, the addition tube 307 is aligned with the test tube slot 311, and then the reagent is added to the inside of the sample test tube through the addition tube 307. Compared to traditional devices, this device, on the one hand, uses a mechanical structure to move the sample test tube below the addition point, achieving precise positioning and reducing human error; on the other hand, it uses pressure sensors to determine whether the addition tube is accurately inserted, stopping operation promptly when the pressure is abnormal to avoid collision damage caused by inaccurate insertion, protecting the sample test tube and the addition tube, reducing equipment costs. Furthermore, accurate insertion of the addition tube ensures smooth reagent delivery and addition, guaranteeing the accuracy and stability of reagent addition, improving addition efficiency, and effectively avoiding sample test tube damage caused by misalignment, thus providing a guarantee for the smooth conduct of nucleic acid extraction experiments.

[0029] The movable component 4 includes connecting plates 402 fixedly installed on both sides of the top of the support plate 1. A rack 401 is fixedly installed on the top of the connecting plate 402. A groove 403 is opened on the outer surface of the connecting plate 402. A placement plate 407 is movably installed on the inner side of the two racks 401. A connecting rod 404 is fixedly installed around the bottom side of the placement plate 407. A gear 406 is rotatably installed on the outer surface of the connecting rod 404, and the gear 406 meshes with the rack 401. A ball bearing 405 is rolled at the bottom end of the connecting rod 404.

[0030] When the staff pulls the placement plate 407 outward with external force, it will cause the connecting rods 404 around the bottom to shift. When the connecting rods 404 shift, they will drive the four gears 406 to move in the direction of meshing with the rack 401. Each rack 401 is equipped with two gears 406. At the same time, the connecting rods 404 will drive the ball bearings 405 at the bottom to roll inside the slide groove 403. This makes it easier to move the reaction vessel placement box 310, thereby increasing the field of view of the test tube slot 311 and making it easier to place sample test tubes. The four connecting rods 404, gears 406 and ball bearings 405 can also increase the stability during movement.

[0031] When the staff pulls the placement plate 407, it causes the bottom connecting rod 404 to shift, which in turn causes multiple gears 406 to move in a specific direction. At the same time, the ball bearing 405 rolls in the slide 403, which together facilitates the convenient movement of the reaction vessel placement box 310 and provides a wide field of vision for the test tube slot 311. Compared with traditional devices, this device greatly facilitates the placement of sample test tubes, reduces operational obstacles, and improves the efficiency of preliminary preparation. Furthermore, the four connecting rods 404, gears 406, and ball bearing 405 work together to form a stable support, which greatly increases the stability during the movement, reduces the risk of sample loss due to shaking, and ensures the smooth conduct of the experiment.

[0032] The inner wall of the adding pipe 307 is fixedly installed with a flow guide 308, and a flow limiting block 309 is fixedly installed on the inner side of the flow guide 308. The bottom of the adding pipe 307 is conical.

[0033] The reagent flows into the inner side of the guide channel 308, and then the guide channel 308 will buffer the flowing reagent to prevent the reagent from being added directly into the sample tube and causing sample splashing. The flow limiting block 309 can effectively limit and block the reagent, so that it can only flow downward through the guide channel 308.

[0034] The fixed bracket 2 has rectangular grooves 9 on both sides inside, and extension blocks 7 are fixedly installed on both sides of the lower pressure plate 302, with the extension blocks 7 extending to the inside of the rectangular grooves 9.

[0035] By extending the extension block 7 to the inside of the rectangular slide groove 9, the rectangular slide groove 9 can limit the extension block 7 when the lower pressure plate 302 moves downward, thereby increasing its stability during movement.

[0036] Among them, a flexible buffer block 8 is fixedly installed on the top of the extension block 7, and the flexible buffer block 8 is located inside the rectangular slide 9.

[0037] A flexible buffer block 8 is fixedly installed on the top of the extension block 7, and the flexible buffer block 8 is located inside the rectangular slide 9. When the lower pressure plate 302 rises, the flexible buffer block 8 can effectively buffer the movement and prevent the lower pressure plate 302 from colliding directly with the inner side of the fixed bracket 2 and causing damage.

[0038] A flexible pad 10 is fixedly installed on the inner side of the test tube trough 311, and the flexible pad 10 is in the shape of a ring.

[0039] The flexible pad 10 is ring-shaped and installed inside the test tube slot 311, thereby flexibly wrapping the sample test tube inside the test tube slot 311 and enhancing its safety.

[0040] The flexible buffer rod 5 is fixedly installed on the side of the placement plate 407, and the baffle 6 is fixedly installed on the top of the bearing plate 1 and is located on one side of one end of the two racks 401.

[0041] The movement of the placement plate 407 will cause the flexible buffer rod 5 to move, and then the baffle 6 will limit the movement of the moving component 4 to prevent it from sliding to the outside. At the same time, the flexible buffer rod 5 can prevent the placement plate 407 from directly hitting the baffle 6, and effectively relieve the force through the flexible buffer rod 5.

[0042] The support plate 1 has a base 11 fixedly installed at its bottom, and the base 11 is fixedly installed around the bottom of the support plate 1.

[0043] By fixing the base 11 to the bottom of the support plate 1 around the perimeter, the stability of the entire device can be effectively increased, avoiding the situation that is easily affected by external physical factors.

[0044] Working principle and usage process of this utility model:

[0045] When the staff places the sample tubes one by one inside the test tube slot 311, and then moves the reaction vessel placement box 310 and the test tube slot 311 to below the fixed bracket 2 via the moving component 4, the electric telescopic rod 301 is activated via the external controller. When the electric telescopic rod 301 is activated, the output shaft will drive the lower pressure plate 302 to gradually move downwards. Then, the lower pressure plate 302 will drive the guide rod 303 to gradually move downwards. At the same time, the guide rod 303 will drive the mounting plate 304 to move downwards together. When the first pressure sensor 305 receives a pressure value greater than the set value, it will... The electric telescopic rod 301 stops operating. Since the pressure value is greater than the set value, it means that the guide rod 303 is not accurately inserted into the guide port 312. At the same time, the adding tube 307 is not aligned with the test tube slot 311. When the guide rod 303 is accurately inserted into the inside of the guide port 312, the first pressure sensor 305 will contact the second pressure sensor 313, thereby allowing the external delivery mechanism to deliver the reagents required for nucleic acid extraction into the storage box 306. At the same time, the adding tube 307 is now aligned with the test tube slot 311, and then the reagent is added to the inside of the sample test tube through the adding tube 307.

[0046] When the staff pulls the placement plate 407 outward with external force, it will cause the connecting rods 404 around the bottom to shift. When the connecting rods 404 shift, they will drive the four gears 406 to move in the direction of meshing with the rack 401. Each rack 401 is equipped with two gears 406. At the same time, the connecting rods 404 will drive the ball bearings 405 at the bottom to roll inside the slide groove 403. This makes it easier to move the reaction vessel placement box 310, thereby increasing the field of view of the test tube slot 311 and making it easier to place sample test tubes. The four connecting rods 404, gears 406 and ball bearings 405 can also increase the stability during movement.

[0047] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0048] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid extraction reagent adding device, comprising a support plate (1), characterized in that: Movable components (4) are fixedly installed on both sides of the top of the support plate (1). A fixed bracket (2) is fixedly installed on the top of the support plate (1). A reagent adding component (3) is fixedly installed inside the fixed bracket (2). The reagent adding component (3) includes an electric telescopic rod (301) fixedly installed inside the fixed bracket (2), and the output end of the electric telescopic rod (301) passes through the fixed bracket (2). A lower pressure plate (302) is fixedly installed at the output end of the electric telescopic rod (301). A guide rod (303) is fixedly installed around the bottom of the lower pressure plate (302). A first pressure sensor (305) is fixedly installed at the end of the guide rod (303). An mounting plate (304) is fixedly installed on the outer surface of the guide rod (303). Both ends of the guide rod (303) pass through the interior of the mounting plate (304). A storage box (306) is fixedly installed on the top of the mounting plate (304). An addition tube (307) is fixedly installed on the bottom side of the mounting plate (304) and the addition tube (307) passes through the mounting plate (304) and communicates with the storage box (306). A reaction container placement box (310) is fixedly installed on the top of the moving component (4). A guide opening (312) is opened around the outer surface of the reaction container placement box (310) and corresponds to the guide rod (303). A second pressure sensor (313) is fixedly installed inside the guide opening (312). A test tube groove (311) is opened on the outer surface of the reaction container placement box (310) and corresponds to the addition tube (307).

2. The nucleic acid extraction reagent addition device according to claim 1, characterized in that: The moving component (4) includes connecting plates (402) fixedly installed on both sides of the top of the support plate (1). A rack (401) is fixedly installed on the top of the connecting plate (402). A groove (403) is opened on the outer surface of the connecting plate (402). A placement plate (407) is movably installed on the inner side of the two racks (401). A connecting rod (404) is fixedly installed around the bottom side of the placement plate (407). A gear (406) is rotatably installed on the outer surface of the connecting rod (404), and the gear (406) meshes with the rack (401). A ball bearing (405) is rolled at the bottom end of the connecting rod (404).

3. The nucleic acid extraction reagent addition device according to claim 1, characterized in that: The inner wall of the adding tube (307) is fixedly installed with a flow guide groove (308), and a flow limiting block (309) is fixedly installed inside the flow guide groove (308), and the bottom of the adding tube (307) is conical.

4. The nucleic acid extraction reagent addition device according to claim 1, characterized in that: The fixed bracket (2) has rectangular grooves (9) on both sides inside. The lower pressure plate (302) has extension blocks (7) fixedly installed on both sides and the extension blocks (7) extend to the inside of the rectangular grooves (9).

5. The nucleic acid extraction reagent addition device according to claim 4, characterized in that: A flexible buffer block (8) is fixedly installed on the top of the extension block (7), and the flexible buffer block (8) is located inside the rectangular groove (9).

6. The nucleic acid extraction reagent addition device according to claim 1, characterized in that: A flexible pad (10) is fixedly installed on the inner side of the test tube groove (311), and the flexible pad (10) is in the shape of a ring.

7. The nucleic acid extraction reagent addition device according to claim 2, characterized in that: A flexible buffer rod (5) is fixedly installed on the side of the placement plate (407), and a baffle (6) is fixedly installed on the top of the bearing plate (1) and located on one side of one end of the two racks (401).

8. The nucleic acid extraction reagent addition device according to claim 1, characterized in that: A base (11) is fixedly installed at the bottom of the support plate (1), and the base (11) is fixedly installed around the bottom of the support plate (1).

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