Microfluidic reagent card convenient to operate

Abstract

The utility model discloses a microfluidic reagent card convenient to operate, which relates to the technical field of microfluidic reagent cards and comprises a lower cover and a replaceable component, the replaceable component is arranged in the lower cover and comprises a sample adding port arranged at the lower right corner of the lower cover, a hollow tube is fixedly connected with an opening at the bottom end of the sample adding port, and the lower end of the hollow tube is provided with a through hole. A hollow-out pipe is arranged at the upper end and the lower end of the hollow-out pipe, a limiting column is fixedly connected between the pipe edges of the upper end and the lower end of the hollow-out pipe, a filter membrane frame is inserted into the side face of the hollow-out pipe, a notch is formed in the outer circle of the filter membrane frame, a filter membrane core is fixed in the filter membrane frame, the section of the sample adding opening is of a funnel-shaped structure, and a hydrophobic coating is additionally arranged on the inner wall of the sample adding opening. According to the microfluidic reagent card convenient to operate, the lower cover structure of a GeneXpert microfluidic nucleic acid detection card box in the prior art is improved, a circular sample adding opening in the prior art is improved into a funnel-shaped structure, and a hydrophobic coating is additionally arranged on the inner wall of the sample adding opening, so that the problem of wall hanging residues during sample adding can be effectively solved.

Description

Technical Field

[0001] This utility model relates to the field of microfluidic reagent card technology, specifically a convenient microfluidic reagent card. Background Technology

[0002] The GeneXpert microfluidic nucleic acid detection cartridge mainly consists of a disposable microfluidic cartridge that carries the entire reaction process of the sample and nucleic acid reagents, and an instrument that works with the cartridge to complete the extraction, amplification and detection actions, ultimately realizing a fully automated nucleic acid analysis function of "sample in - result out".

[0003] The GeneXpert microfluidic nucleic acid detection cartridge mainly consists of a lid, reagent chamber, base, and reaction tube on the side. The lid structure includes an upper cover and a lower cover, which are connected by a hinge. The center of the lower cover is the insertion port for the plunger on the instrument, which, together with the piston inside the cartridge, forms a plunger pump assembly. The vents for each reagent chamber are distributed in a circle around the plunger inlet. The sample dispensing port is located in the lower right corner. Through the sample dispensing port, the filter membrane fixed at the bottom of the sample chamber can be observed to filter sample impurities. However, the sample dispensing port of the GeneXpert microfluidic nucleic acid detection cartridge has a circular structure, which makes it easy for sample residue to adhere to the wall. Furthermore, the filter membrane equipped with the sample dispensing port is fixed, which cannot flexibly change the pore size filter membrane for different sample types such as sputum and blood. A single fixed filter membrane is prone to clogging or loss of effective components. Utility Model Content

[0004] The purpose of this invention is to provide a convenient microfluidic reagent card to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a convenient microfluidic reagent card, comprising a lower cover and a replaceable component. The lower cover contains the replaceable component, which includes a sample dispensing port located at the lower right corner of the lower cover. A perforated tube is fixedly connected to the bottom opening of the sample dispensing port, and a limiting post is fixedly connected between the upper and lower ends of the perforated tube. A filter membrane frame is inserted into the side of the perforated tube, and a notch is provided on the outer circumference of the filter membrane frame. A filter membrane core is fixed inside the filter membrane frame.

[0006] Furthermore, the sample inlet has a funnel-shaped cross-section, and a hydrophobic coating is added to the inner wall of the sample inlet.

[0007] Furthermore, a plunger inlet is provided in the middle of the lower cover, and air holes are distributed in an array on the outer circle of the plunger inlet.

[0008] Furthermore, a sealing ring is provided at the bottom of the lower cover, and a locking block protrudes from the edge of the lower cover.

[0009] Furthermore, a hinge is connected to the side of the lower cover, and an upper cover is rotatably mounted on the outside of the hinge.

[0010] Furthermore, the upper cover has through holes corresponding to the lower cover, and the upper surface of the through holes is covered with a reagent chamber sealing film.

[0011] Furthermore, a slot is provided at the edge of the upper cover to fit tightly with the card block, and a sealing ring is provided at the upper right corner of the upper cover to fit tightly with the sample dispensing port.

[0012] Furthermore, the bottom of the lower cover is fastened to the reagent kit by a sealing ring, and the bottom of the reagent kit is connected to a base.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] 1. This utility model improves the fixed filter membrane of the GeneXpert cartridge into a drawer-type replaceable filter membrane core structure. The filter membrane frame is inserted into the side of the hollow tube and locked in place by the cooperation of the limiting post and the notch. This allows the filter membrane with different pore sizes to be flexibly changed for different sample types such as sputum and blood (e.g., 5μm filter membrane to block blood cells, 40μm filter membrane to filter sputum mucoprotein), avoiding clogging or loss of effective components caused by a single fixed filter membrane. In addition, the filter membrane core can be inserted and removed separately after clogging, without discarding the whole cartridge, reducing the detection cost.

[0015] 2. This utility model improves upon the existing GeneXpert microfluidic nucleic acid detection cartridge's lower cover structure by changing the existing circular sample dispensing port to a funnel-shaped structure and adding a hydrophobic coating to the inner wall of the sample dispensing port, which can effectively solve the problem of sample residue sticking to the wall during sample dispensing. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the device of this utility model;

[0017] Figure 2 This is a schematic diagram of the split structure of the device of this utility model;

[0018] Figure 3 This is a schematic diagram of the replaceable component structure of this utility model.

[0019] In the diagram: 1. Lower cover; 2. Replaceable component; 201. Sample dispensing port; 202. Hollow tube; 203. Limiting column; 204. Filter membrane frame; 205. Notch; 206. Filter membrane core; 3. Piston inlet; 4. Vent; 5. Sealing ring; 6. Locking block; 7. Hinge; 8. Upper cover; 9. Through hole; 10. Slot; 11. Sealing ring; 12. Reagent kit; 13. Base. Detailed Implementation

[0020] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0021] like Figures 1 to 3 As shown, a convenient microfluidic reagent card includes a lower cover 1 and a replaceable component 2. The lower cover 1 has the replaceable component 2 built in. The replaceable component 2 includes a sample dispensing port 201 opened at the lower right corner of the lower cover 1. A hollow tube 202 is fixedly connected to the bottom opening of the sample dispensing port 201. A limiting post 203 is fixedly connected between the upper and lower ends of the hollow tube 202. A filter membrane frame 204 is inserted into the side of the hollow tube 202. A notch 205 is provided on the outer circle of the filter membrane frame 204. A filter membrane core 206 is fixed inside the filter membrane frame 204.

[0022] The specific operation is as follows: This application improves the fixed filter membrane of the GeneXpert cartridge to a drawer-type replaceable filter membrane core 206 structure. The filter membrane frame 204 is inserted into the side of the hollow tube 202 and is locked and limited by the cooperation of the limiting post 203 and the notch 205. This allows the filter membrane with different pore sizes to be flexibly replaced for different sample types such as sputum and blood, avoiding clogging or loss of effective components caused by a single fixed filter membrane. In addition, the filter membrane core 206 can be inserted and removed separately after clogging, without discarding the whole card, thus reducing the detection cost.

[0023] like Figures 1 to 2 As shown, the sample inlet 201 has a funnel-shaped cross-section and a hydrophobic coating on the inner wall of the sample inlet 201. The lower cover 1 has a plunger inlet 3 in the middle and air holes 4 are distributed in an array on the outer circle of the plunger inlet 3. The bottom of the lower cover 1 is provided with a sealing ring 5 and a locking block 6 protrudes from the edge of the lower cover 1. The side of the lower cover 1 is connected to a hinge 7 and an upper cover 8 is rotatably installed on the outside of the hinge 7. The upper cover 8 has through holes 9 corresponding to the lower cover 1 and the upper surface of the through holes 9 is covered with a reagent chamber sealing film. The edge of the upper cover 8 is provided with a slot 10 that fits tightly with the locking block 6, and the upper right corner of the upper cover 8 is provided with a sealing ring 11 that fits tightly with the sample inlet 201. The bottom of the lower cover 1 is fastened to the reagent kit 12 by the sealing ring 5, and the bottom of the reagent kit 12 is connected to a base 13.

[0024] The specific operation is as follows: This application improves the structure of the lower cover 1 of the GeneXpert microfluidic nucleic acid detection cartridge based on the existing technology. The circular sample dispensing port 201 of the existing technology is improved into a funnel-shaped structure, and a hydrophobic coating is added to the inner wall of the sample dispensing port 201, which can effectively solve the problem of residue on the wall when the sample is dispensed.

[0025] It is worth noting that this application is an improvement and optimization of the lower cover 1 structure of the GeneXpert microfluidic nucleic acid detection cartridge. Its working principle is the same as that of the cartridge, which can be summarized as follows: Sample addition: The sample is injected into the cartridge, and the filter membrane filters out impurities; Lysis: The instrument drives the sample to mix with the lysis buffer, and the cells are broken to release nucleic acid; Amplification: The nucleic acid is amplified by real-time fluorescent PCR in a sealed reaction tube; Detection: The optical module analyzes the fluorescence signal and automatically outputs the results.

[0026] Working Principle: This application improves upon the existing GeneXpert microfluidic nucleic acid detection cartridge's lower cover 1 structure by modifying the existing circular sample inlet 201 into a funnel-shaped structure and adding a hydrophobic coating to the inner wall of the sample inlet 201. This effectively solves the problem of sample residue adhering to the wall during sample addition. This application improves the GeneXpert cartridge's fixed filter membrane into a drawer-type replaceable filter membrane core 206 structure. The filter membrane frame 204 is inserted from the side of the hollow tube 202 and locked in place by the cooperation of the limiting post 203 and the notch 205. This allows for flexible replacement of filter membranes with different pore sizes for different sample types such as sputum and blood (e.g., a 5μm filter membrane to block blood cells, and a 40μm filter membrane to filter sputum mucoproteins), avoiding clogging or loss of effective components caused by a single fixed filter membrane. In addition, the filter membrane core 206 can be individually inserted and removed for replacement after clogging, eliminating the need to discard the entire cartridge and reducing testing costs.

[0027] The embodiments of this utility model are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the utility model to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical applications of this utility model, and to enable those skilled in the art to understand this utility model and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A user-friendly microfluidic reagent card, characterized in that, The device includes a lower cover (1) and a replaceable component (2). The lower cover (1) contains the replaceable component (2). The replaceable component (2) includes a sample inlet (201) located at the lower right corner of the lower cover (1). A perforated tube (202) is fixedly connected to the bottom opening of the sample inlet (201). A limiting post (203) is fixedly connected between the upper and lower ends of the perforated tube (202). A filter membrane frame (204) is inserted into the side of the perforated tube (202). A notch (205) is provided on the outer circle of the filter membrane frame (204). A filter membrane core (206) is fixed inside the filter membrane frame (204).

2. The convenient microfluidic reagent card according to claim 1, characterized in that, The sample inlet (201) has a funnel-shaped cross-section, and a hydrophobic coating is added to the inner wall of the sample inlet (201).

3. The convenient microfluidic reagent card according to claim 2, characterized in that, The lower cover (1) has a plunger inlet (3) in the middle, and the plunger inlet (3) has air holes (4) arranged in an array on the outer circle.

4. The convenient microfluidic reagent card according to claim 3, characterized in that, The bottom of the lower cover (1) is provided with a sealing ring (5), and the edge of the lower cover (1) has a protruding locking block (6).

5. The convenient microfluidic reagent card according to claim 4, characterized in that, The lower cover (1) is connected to a hinge (7) on its side, and an upper cover (8) is rotatably mounted on the outside of the hinge (7).

6. The convenient microfluidic reagent card according to claim 5, characterized in that, The upper cover (8) has through holes (9) corresponding to the lower cover (1), and the upper surface of the through holes (9) is covered with a reagent chamber sealing film.

7. A convenient microfluidic reagent card according to claim 6, characterized in that, The upper cover (8) has a slot (10) at its edge that fits tightly with the card block (6), and a sealing ring (11) is provided at the upper right corner of the upper cover (8) that fits tightly with the sample inlet (201).

8. A convenient microfluidic reagent card according to claim 7, characterized in that, The bottom cover (1) is fastened to the reagent kit (12) by a sealing ring (5), and the bottom of the reagent kit (12) is connected to a base (13).

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