An extraction device for mass spectrometry analysis and detection of microorganisms

Abstract

This invention discloses an extraction device for mass spectrometry analysis and detection of microorganisms, comprising a first piston cylinder, one end of which is threadedly connected to a first end cap, and the other end of which is threadedly connected to a second end cap. A sampling head is connected to one end of the first end cap. A first piston block is slidably connected inside the first piston cylinder. A second piston cylinder is connected to one end of the second end cap, and a second piston block is connected inside the second piston cylinder. A first threaded rod is provided at one end of the second piston block, and a limit block is connected to the outer side of the first threaded rod. A second threaded rod is rotatably connected inside the first threaded rod. A rectangular outer shell is provided on the outer side of the second piston cylinder, and a rectangular piston block is slidably connected inside the rectangular outer shell. A third threaded rod is threadedly connected inside the rectangular piston block. This invention enables convenient quantitative extraction of microorganisms and facilitates the quantitative dispensing of microorganisms into different containers for parallel analysis, thereby improving the accuracy of microbial detection.

Description

Technical Field

[0001] This utility model relates to the field of microbial extraction technology, and in particular to an extraction device for mass spectrometry analysis and detection of microorganisms. Background Technology

[0002] In the field of microbial detection, mass spectrometry has become a promising analytical method due to its high sensitivity, high resolution, and rapid accuracy. By analyzing specific components (such as proteins and metabolites) in microbial samples using mass spectrometry, precise identification of microbial species and strains can be achieved, playing a crucial role in numerous fields such as clinical diagnosis, food safety testing, and environmental monitoring. However, the sample extraction stage is critical in the actual operation of mass spectrometry for microbial detection. Current techniques typically use syringes to extract microorganisms. However, when using syringes, the amount of microorganisms extracted is usually determined by visually observing the alignment of the liquid level with the graduation line. This method is highly susceptible to extracting too many or too few microorganisms, significantly affecting the accuracy of the detection. Furthermore, after sample extraction, it is often necessary to aliquot the samples into different containers for subsequent processing or parallel analysis of multiple samples. Using syringes also makes it difficult to quantitatively aliquot microorganisms, thus affecting the accuracy of the detection. Utility Model Content

[0003] The purpose of this invention is to provide an extraction device for mass spectrometry analysis and detection of microorganisms, which can conveniently extract microorganisms quantitatively and also conveniently dispense microorganisms into different containers for parallel analysis, thereby improving the accuracy of microbial detection.

[0004] The above-mentioned technical objective of this utility model is achieved through the following technical solution:

[0005] An extraction device for mass spectrometry analysis and detection of microorganisms includes a first piston cylinder, one end of which is threadedly connected to a first end cap, and the other end of which is threadedly connected to a second end cap. A sampling head is connected to the end of the first end cap away from the second end cap. A first piston block is slidably connected inside the first piston cylinder. A second piston cylinder is connected to the end of the second end cap away from the first end cap. The second piston cylinder is rotatably connected to the second piston block.

[0006] The second piston block has a first threaded rod that is threadedly connected to the second piston cylinder at one end away from the second end cover. The outer side of the first threaded rod is slidably connected to a limit block inside the second piston cylinder. The inner side of the first threaded rod is rotatably connected to a second threaded rod. The outer side of the first threaded rod has multiple limit sliding openings that are equally spaced. The inner side of the limit block has multiple threaded blocks that correspond one-to-one with the limit sliding openings and are slidably connected. Each threaded block is threadedly connected to the second threaded rod.

[0007] The outer side of the second piston cylinder is provided with a rectangular outer shell, and a rectangular piston block is slidably connected inside the rectangular outer shell. The inner side of the rectangular piston block is threadedly connected with a third threaded rod that is slidably connected to the rectangular outer shell. A limiting block that is slidably connected to the rectangular outer shell is provided at one end of the third threaded rod near the end cap. A first conduit is connected between the rectangular outer shell and the second end cap. A first one-way valve is provided at one end of the first conduit. A second conduit is connected to the outer side of the rectangular outer shell near the first conduit. A second one-way valve is provided at one end of the second conduit.

[0008] By adopting the above technical solution, in use, the first and second end caps are connected to the piston cylinder. Rotating the first threaded rod, since it is threadedly connected to the second piston cylinder, causes the first threaded rod to move relative to the second piston cylinder, thereby causing the second piston block to slide relative to the second piston cylinder. This draws gas from the first piston cylinder into the second piston cylinder, creating negative pressure. This causes the first piston block to move relative to the first piston cylinder, drawing microorganisms through the sampling head into the first piston cylinder. When the first threaded rod drives the limiting block to abut against the second piston cylinder, the extraction stops. By turning the second threaded rod relative to the first threaded rod, the second threaded rod drives the threaded block to slide relative to the limiting slide, thereby causing the limiting block to slide relative to the first threaded rod. Changing the position of the limiting block changes the distance the first threaded rod moves when the limiting block abuts against the second piston cylinder, thus changing the amount of gas extracted from the first piston cylinder. The method changes the extraction volume to quantitatively extract microorganisms. During quantitative dispensing, pushing the third threaded rod causes a rectangular piston block to slide relative to the rectangular outer shell. Gas in the rectangular shell passes through the first one-way valve and then through the first conduit into the first piston cylinder, causing the first piston block to slide relative to the first piston cylinder. Microorganisms are then discharged from the first piston cylinder through the sampling head. When the limiting block abuts against the rectangular outer shell, pushing the third threaded rod stops. Then, pulling the third threaded rod moves the rectangular piston block, extracting gas through the second conduit and then through the second one-way valve into the rectangular outer shell. By repeatedly pushing and pulling the third threaded rod, microorganisms in the first piston cylinder are quantitatively dispensed. Rotating the third threaded rod moves it relative to the rectangular piston block, causing the limiting block to move. Changing the distance the rectangular piston block can move changes the amount of gas entering the first piston cylinder, thus altering the dispensing volume of microorganisms.

[0009] A further feature of this invention is that both the first piston cylinder and the second piston cylinder are transparent.

[0010] A further feature of this invention is that: multiple limiting strips are equally spaced on the inner side of the first piston cylinder, and multiple limiting slots corresponding to and slidably connected to the limiting strips are opened on the outer side of the first piston block.

[0011] A further feature of this invention is that a first knob is provided at the outer end of the first threaded rod away from the second piston block, and a second knob is provided at the outer end of the second threaded rod away from the second piston block.

[0012] A further feature of this invention is that a scale line is provided on the outer side of the second piston cylinder.

[0013] A further feature of this invention is that a third knob is provided at one end of the third threaded rod away from the limiting block, and a return spring is provided on the outer side of the third threaded rod between the third knob and the rectangular outer shell.

[0014] By adopting the above technical solution, the third knob can be easily reset, thereby resetting the rectangular piston block.

[0015] In summary, this utility model has the following beneficial effects: Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a partial sectional view of the present invention;

[0018] Figure 3 This demonstrates the connection relationship between the first end cap, the first piston cylinder, and the second end cap of this utility model;

[0019] Figure 4 This is to demonstrate the limiting slide and threaded locking block of this utility model.

[0020] In the diagram: 1. First piston cylinder; 2. First end cap; 3. Sampling head; 4. First piston block; 5. Limiting slot; 6. Limiting strip; 7. Second end cap; 8. Second piston cylinder; 9. Second piston block; 10. First threaded rod; 11. Limiting slide; 12. First knob; 13. Second threaded rod; 14. Second knob; 15. Limiting block; 16. Threaded block; 17. Rectangular outer shell; 18. Rectangular piston block; 19. Third threaded rod; 20. Third knob; 21. Return spring; 22. Limiting block; 23. First guide tube; 24. First one-way valve; 25. Second guide tube; 26. Second one-way valve; 27. Scale line. Detailed Implementation

[0021] The present invention will be further described in detail below with reference to the accompanying drawings.

[0022] In the description of this utility model, it should be understood that the terms "upper", "lower", "left", "right", "front", "rear", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0023] Furthermore, terms such as "horizontal" and "vertical" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0024] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set up," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, a direct connection, or an indirect connection through an intermediate medium; or they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0025] Example, refer to Figure 1-4 An extraction device for mass spectrometry analysis and detection of microorganisms includes a first piston cylinder 1, a first end cap 2 threadedly connected to the lower end of the first piston cylinder 1, a second end cap 7 threadedly connected to the upper end of the first piston cylinder 1, and a sampling head 3 connected to the middle of the lower end of the first end cap 2, which allows for easy disassembly and installation of the first end cap 2 and the second end cap 7.

[0026] A first piston block 4 is slidably connected inside the first piston cylinder 1. Two limiting strips 6 are equally spaced inside the first piston cylinder 1. Two limiting slots 5, which correspond one-to-one with the limiting strips 6 and are slidably connected, are equally spaced on the outer side of the first piston block 4. This can improve the stability of the first piston block 4 when sliding in the first piston cylinder 1. The upper end of the second end cap 7 is connected to a vertically arranged second piston cylinder 8. A scale line 27 is provided on the outer side of the second piston cylinder 8. Both the first piston cylinder 1 and the second piston cylinder 8 are transparent. The amount of air drawn into the first piston cylinder 1 can be easily observed to determine the amount of microorganisms drawn.

[0027] A second piston block 9 is rotatably and slidably connected inside the second piston cylinder 8. A first threaded rod 10, threadedly connected to the second piston cylinder 8, is vertically arranged at the upper end of the second piston block 9. A second threaded rod 13, also vertically arranged, is rotatably connected inside the first threaded rod 10. A limiting block 15 is movably connected to the outside of the first threaded rod 10 inside the first piston cylinder 1. Two equally spaced limiting sliding openings 11 are provided on the outside of the first threaded rod 10. Two equally spaced limiting sliding openings 11 are provided inside the limiting block 15. Each of the threaded blocks 16, which are slidably connected to the first threaded rod 13, is threadedly connected to the second threaded rod 13. A first knob 12 is provided at the upper end of the outer side of the first threaded rod 10, and a second knob 14 is provided at the upper end of the second threaded rod 13. This allows for easy adjustment of the position of the limiting block 15, thereby adjusting the distance that the second piston block 9 can move relative to the second piston cylinder 8, changing the amount of gas extracted from the first piston cylinder 1, and thus changing the movement of the first piston block 4, thereby increasing the amount of microorganisms extracted, which facilitates quantitative extraction.

[0028] A rectangular outer shell 17 is vertically arranged on the outer side of the second piston cylinder 8. A rectangular locking block is slidably connected inside the outer shell of the rectangle. A third threaded rod 19 is threadedly connected inside the rectangular locking block and rotates and slides with the rectangular outer shell 17. A limiting block 22 is provided at the lower end of the third threaded rod 19. A third knob 20 is provided at the upper end of the third threaded rod 19. A return spring 21 is provided between the third knob 20 and the rectangular outer shell 17 on the outer side of the third threaded rod 19, which can easily adjust the rectangular piston block 18 to return to its original position. A first conduit 23 is connected between the right side of the lower end of the rectangular outer shell 17 and the second end cap 7. A first one-way valve 24 is provided at the upper end of the first conduit 23. A second conduit 25 is connected to the left side of the lower end of the rectangular outer shell 17. A second one-way valve 26 is provided at the upper end of the second conduit 25, which can easily quantitatively dispense the microorganisms in the first piston cylinder 1 and can also easily adjust the dispensing amount as needed.

[0029] Usage: In use, connect the first end cap 2 and the second end cap 7 to the piston cylinder. By rotating the first knob 12, the first threaded rod 10 is rotated. Since the first threaded rod 10 is threadedly connected to the second piston cylinder 8, it moves relative to the second piston cylinder 8, thereby causing the second piston block 9 to slide relative to the second piston cylinder 8. This draws gas from the first piston cylinder 1 into the second piston cylinder 8, creating negative pressure. This causes the first piston block 4 to move relative to the first piston cylinder 1. The first piston block 4 then causes the limiting jaw 5 to slide relative to the limiting strip 6, drawing microorganisms through the sampling head 3 into the first piston cylinder 1. When the first threaded rod 10 drives the limiting block 15 to abut against the second piston cylinder 8, extraction stops. By rotating the second knob 14, the second threaded rod 13 rotates relative to the first threaded rod 10. The second threaded rod 13 drives the threaded block 16 to slide relative to the limiting slide 11, thereby driving the limiting block 15 to slide relative to the first threaded rod 10. Changing the position of the limiting block 15 changes the distance the first threaded rod 10 drives the piston block to move when the limiting block 15 abuts against the second piston cylinder 8. This changes the amount of gas extracted from the first piston cylinder 1. Changing the distance the first piston block 4 moves changes the extraction amount, thus allowing for quantitative extraction of microorganisms.

[0030] When quantitatively dispensing microorganisms, pushing the third knob 20 causes the third threaded rod 19 to slide the rectangular piston block 18 relative to the rectangular outer shell 17, compressing the return spring 21. The gas in the rectangular outer shell 17 passes through the first one-way valve 24 and then enters the first piston cylinder 1 through the first conduit 23, causing the first piston block 4 to slide relative to the first piston cylinder 1, dispensing the microorganisms from the first piston cylinder 1 through the sampling head 3. When the limiting block 22 abuts against the rectangular outer shell 17, pushing the third threaded rod 19 stops, and then the return spring 21 releases the gas. The third knob 20 is reset, which in turn moves the rectangular piston block 18, drawing gas through the second conduit 25 and then through the second one-way valve 26 into the rectangular outer shell 17. By repeatedly pushing and pulling the third threaded rod 19, the microorganisms in the first piston cylinder 1 are quantitatively dispensed. By rotating the third threaded rod 19, the third threaded rod 19 moves relative to the rectangular piston block 18, which moves the limiting block 22. Changing the distance that can move the rectangular piston block 18 changes the amount of gas entering the first piston cylinder 1, thereby changing the amount of microorganisms dispensed.

[0031] This specific embodiment is merely an explanation of the present utility model and is not intended to limit the present utility model. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but as long as they are within the scope of the claims of the present utility model, they are protected by patent law.

Claims

1. An extraction device for mass spectrometry analysis and detection of microorganisms, comprising a first piston cylinder (1), characterized in that: One end of the first piston cylinder (1) is threadedly connected to a first end cap (2), and the other end of the first piston cylinder (1) is threadedly connected to a second end cap (7). The end of the first end cap (2) away from the second end cap (7) is connected to a sampling head (3). The first piston cylinder (1) is slidably connected to a first piston block (4). The end of the second end cap (7) away from the first end cap (2) is connected to a second piston cylinder (8). The second piston cylinder (8) is rotatably connected to a second piston block (9). The second piston block (9) is provided with a first threaded rod (10) that is threaded to the second piston cylinder (8) at one end away from the second end cap (7). The outer side of the first threaded rod (10) is slidably connected to a limit block (15) inside the second piston cylinder (8). The inner side of the first threaded rod (10) is rotatably connected to a second threaded rod (13). The outer side of the first threaded rod (10) has multiple limit sliding openings (11) that are equally spaced. The inner side of the limit block (15) has multiple threaded blocks (16) that correspond one-to-one with the limit sliding openings (11) and are slidably connected. Each threaded block (16) is threadedly connected to the second threaded rod (13). A rectangular shell (17) is provided on the outside of the second piston cylinder (8). A rectangular piston block (18) is slidably connected inside the rectangular shell (17). A third threaded rod (19) is slidably connected to the rectangular shell (17) inside the rectangular piston block (18). A limit block (22) is provided at one end of the third threaded rod (19) near the second end cap (7). A first conduit (23) is connected between the rectangular shell (17) and the second end cap (7). A first check valve (24) is provided at one end of the first conduit (23). A second conduit (25) is connected to the outside of the rectangular shell (17) near the first conduit (23). A second check valve (26) is provided at one end of the second conduit (25).

2. The extraction device for mass spectrometry analysis and detection of microorganisms according to claim 1, characterized in that: Both the first piston cylinder (1) and the second piston cylinder (8) are transparent.

3. The extraction device for mass spectrometry analysis and detection of microorganisms according to claim 1, characterized in that: The inner side of the first piston cylinder (1) is provided with multiple limiting strips (6) at equal intervals, and the outer side of the first piston block (4) is provided with multiple limiting slots (5) that correspond one-to-one with the limiting strips (6) and are slidably connected.

4. The extraction device for mass spectrometry analysis and detection of microorganisms according to claim 1, characterized in that: A first knob (12) is provided at the outer end of the first threaded rod (10) away from the second piston block (9), and a second knob (14) is provided at the outer end of the second threaded rod (13) away from the second piston block (9).

5. The extraction device for mass spectrometry analysis and detection of microorganisms according to claim 1, characterized in that: The outer side of the second piston cylinder (8) is provided with scale lines (27).

6. The extraction device for mass spectrometry analysis and detection of microorganisms according to claim 1, characterized in that: A third knob (20) is provided at one end of the third threaded rod (19) away from the limiting block (22), and a return spring (21) is provided on the outer side of the third threaded rod (19) between the third knob (20) and the rectangular outer shell (17).

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