A cell culture device for biological experiments
By designing a cell culture device with a central column, elastic flaps, and flexible scrapers, the problems of liquid splashing and positional displacement during reagent mixing in traditional cell culture devices have been solved. This achieves uniform reagent distribution and stable support, improving the accuracy and efficiency of experiments.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- THE FIRST AFFILIATED HOSPITAL OF HAINAN MEDICAL UNIV
- Filing Date
- 2025-06-25
- Publication Date
- 2026-05-26
AI Technical Summary
Existing cell culture devices require manual mixing after adding CCK8 reagent, which can easily lead to liquid splashing or contamination. Furthermore, they lack precise positioning and stable support for the cell culture plate, increasing experimental errors.
A cell culture device was designed, comprising a culture dish body, a cover, and a distribution component. The distribution component consists of a central column, an elastic flap, and a flexible scraper. The uniform distribution of reagents is achieved by rotating the cover, and the initial distribution is optimized by a conical guide head and a guide groove. The device stability is ensured by combining an anti-slip base and a suction cup.
It achieves uniform distribution of reagents, reduces experimental errors, minimizes reagent waste, and improves the reliability and accuracy of experimental results. The device is also highly stable and easy to operate.
Smart Images
Figure CN224280310U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to a cell culture device for biological experiments. Background Technology
[0002] In biological experiments, cell proliferation and toxicity analysis are important methods for studying cell biological behavior. The CCK8 (Cell Counting Kit-8) reagent is widely used in cell proliferation and toxicity analysis experiments due to its ease of use and accurate results. Its basic principle is that the tetrazolium salt WST-8 in the reagent is reduced by dehydrogenases in the cell mitochondria under the action of the electron carrier 1-Methoxy PMS to generate a yellow formazan product. The amount of formazan produced is directly proportional to the number of viable cells, thus enabling quantitative analysis of cell proliferation or toxicity.
[0003] However, in actual experimental operations, existing cell culture devices have some shortcomings that affect experimental efficiency and the accuracy of results. For example, traditional cell culture devices often employ an open design or a simple closed design. After adding CCK8 reagent, manual mixing is required. Improper operation during this process can easily lead to liquid splashing or contamination, thus adversely affecting the reliability of experimental data. In addition, existing devices lack precise positioning and stable support for cell culture plates, which can easily cause positional shifts or uneven liquid distribution when operating multi-well plates, further increasing experimental errors.
[0004] Therefore, it is necessary to design a cell culture device specifically designed for CCK8 experiments to solve the above problems and improve the convenience, accuracy and reliability of experimental operations. Utility Model Content
[0005] The purpose of this invention is to provide a cell culture device for biological experiments.
[0006] The technical solution adopted in this utility model is as follows:
[0007] A cell culture device for biological experiments includes a culture dish body and a cover detachably connected to its top. A distribution component for uniformly distributing reagents is installed at the bottom of the cover. The distribution component includes a central column fixedly connected to the inner wall of the cover. A plurality of elastic tabs are arranged in a circumferential array around the outer ring of the central column. A flexible scraper is connected to the end of each elastic tab. An anti-slip base is provided at the bottom of the culture dish body.
[0008] Specifically, a conical guide head is fixedly connected to the end of the central column away from the cover, and multiple guide grooves are opened on the outer ring of the conical guide head along its circumference.
[0009] Furthermore, the elastic lever is fixedly connected at the gap between two adjacent guide channels.
[0010] Furthermore, the surface of the elastic lever is provided with multiple ventilation holes along its length.
[0011] Furthermore, the inner wall of the culture dish body is provided with an annular groove, and the end of the flexible scraper contacts the inner wall of the annular groove.
[0012] Furthermore, the bottom of the anti-slip base is connected to multiple suction cups, which are connected to the anti-slip base by threads.
[0013] Preferably, the petri dish body is made of a transparent material.
[0014] Preferably, the outer ring of the cover is provided with multiple transverse anti-slip protrusions.
[0015] Compared with the prior art, the present invention has the following beneficial effects:
[0016] (1) This utility model utilizes the coordinated action of the central column, elastic paddle and flexible scraper in the distribution component. When the cover rotates, the elastic paddle rotates synchronously with the central column, driving the flexible scraper to move along the inner wall of the culture dish body, effectively dispersing the reagent evenly to the bottom of the culture dish body, avoiding the problem of local reagent accumulation caused by improper operation during traditional manual mixing, and reducing experimental errors. At the same time, the flexible scraper can scrape off the reagent residue on the inner wall during the movement, reducing reagent waste.
[0017] (2) In this utility model, the design of the conical guide head and the guide groove further optimizes the initial distribution effect of the reagent. When the reagent is dropped into the main body of the culture dish, the inclined surface of the conical guide head can guide the reagent into the circumferentially opened guide groove. The guide groove guides the reagent to be evenly dispersed along the groove to the bottom of the main body of the culture dish, realizing the initial uniform distribution of the reagent. This provides a more uniform initial state for the secondary mixing of the elastic lever, reduces the dispersion of experimental data, and ensures the reliability of cell proliferation or toxicity analysis results in the CCK8 experiment.
[0018] (3) In this utility model, the contact and cooperation between the annular groove on the inner wall of the petri dish and the flexible scraper effectively solves the problem of reagent residue. The end of the flexible scraper slides along the inner wall of the annular groove to accurately scrape off the reagent residue in the groove, reducing reagent waste and avoiding the risk of deterioration or contamination of the residual reagent due to long-term exposure, further ensuring the accuracy of the experimental results.
[0019] (4) In this utility model, the threaded connection design of the anti-slip base and the suction cup improves the stability and adaptability of the device. The suction cup can be adjusted in position or replaced with different types according to the material and roughness of the experimental table surface to ensure that the device is tightly attached to the table surface. The threaded connection makes the disassembly and replacement of the suction cup simple and does not require additional tools.
[0020] (5) In this invention, the main body of the culture dish is made of transparent material. Experimenters can directly observe the distribution of reagents and cell growth inside the culture dish through the transparent wall. This intuitive observation method avoids the damage to the experimental environment caused by frequent opening of the lid for inspection, and at the same time facilitates the real-time recording of experimental data (such as taking pictures or observing under a microscope). Attached Figure Description
[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model.
[0022] Figure 2 This is a partial enlarged view of the distribution component of this utility model.
[0023] Figure 3 This is a schematic diagram of the bottom structure of the petri dish body of this utility model.
[0024] The component names corresponding to the reference numerals in the attached drawings are as follows:
[0025] 1. Petri dish body; 2. Cover; 3. Distribution assembly; 4. Central column; 5. Elastic paddle; 6. Flexible scraper; 7. Conical guide head; 8. Guide groove; 9. Ventilation hole; 10. Annular groove; 11. Anti-slip base; 12. Suction cup; 13. Lateral anti-slip protrusion. Detailed Implementation
[0026] The following is in conjunction with the appendix Figure 1 To be continued Figure 3 The specific embodiments of this utility model are described in detail with reference to the component numbers marked in the accompanying drawings.
[0027] like Figure 1As shown, the cell culture device of this invention includes a culture dish body 1 and a cover 2. The cover 2 is detachably connected to the top of the culture dish body 1. This detachable connection can be achieved through threads, snaps, or other common connection methods, facilitating quick assembly or disassembly of the device for operation. A distribution component 3 is installed at the bottom of the cover 2, which is used to achieve uniform distribution of reagents during sample addition. An anti-slip base 11 is provided at the bottom of the culture dish body 1. The bottom of the anti-slip base 11 is connected to multiple suction cups 12 by threads. These suction cups 12 can firmly adhere to the experimental table when the device is placed, enhancing overall stability and effectively preventing reagent spillage or contamination caused by accidental collisions. In addition, the outer ring of the cover 2 is provided with multiple transverse anti-slip protrusions 13. These protrusions not only increase the friction when handling the device, but also prevent the cover 2 from accidentally slipping off the culture dish body 1. The transverse anti-slip protrusions 13 are integrally molded from the same material as the cover 2, with a width of approximately 3mm to 5mm and a height of approximately 1mm to 2mm. Their surface can be designed with fine textures to further increase the coefficient of friction.
[0028] Further integration Figure 2 The distribution component 3 includes a central column 4, elastic levers 5, flexible scrapers 6, a conical guide head 7, flow channels 8, and vent holes 9. The central column 4 is fixedly connected to the center of the inner wall of the cover 2, serving as the core support structure of the distribution component 3. A conical guide head 7 is fixedly connected to the end of the central column 4 away from the cover 2. Multiple flow channels 8 are circumferentially formed on the outer ring of the conical guide head 7. These flow channels 8 are designed to guide the reagent to the bottom of the culture dish body 1 during reagent dripping and achieve initial uniform distribution through the tilt angle of the flow channels 8. Elastic levers 5 are fixedly connected in the gap between adjacent flow channels 8. The elastic levers 5 are arranged in a circumferential array along the central column 4, and a flexible scraper 6 is connected to the end of each elastic lever 5. The end of the flexible scraper 6 contacts the annular groove 10 on the inner wall of the petri dish body 1. When the cover 2 rotates, the elastic lever 5 drives the flexible scraper 6 to move along the inner wall of the annular groove 10, thereby scraping away residual reagents and reintroducing them into the bottom area of the petri dish body 1, effectively reducing reagent waste. Furthermore, the surface of the elastic lever 5 has multiple vent holes 9 along its length. These vent holes 9 allow airflow during rotation, preventing uneven reagent distribution due to airflow blockage. The number of flow channels 8 is 6 to 8, with an inclination angle between 30° and 45°. The specific values are designed based on the diameter of the petri dish body 1 and the viscosity of the reagent to ensure optimal flow guidance.
[0029] The inner wall of the culture dish body 1 is provided with an annular groove 10, which serves to provide a contact surface for the flexible scraper 6, ensuring that the flexible scraper 6 can closely adhere to the inner wall of the culture dish body 1 during rotation. The depth and width of the annular groove 10 are precisely designed to ensure the effective scraping function of the flexible scraper 6 without affecting the overall structural strength of the culture dish body 1. The culture dish body 1 is made of polystyrene, allowing researchers to directly observe the distribution of reagents and the cell culture status. The wall thickness of the culture dish body 1 is controlled between 2mm and 4mm to ensure sufficient mechanical strength while maintaining good optical transparency.
[0030] like Figure 3 As shown, the bottom of the anti-slip base 11 is connected to multiple suction cups 12 via threads. The number of suction cups 12 is 4 to 6, and the diameter is 20mm to 30mm. They are evenly distributed on the edge area of the anti-slip base 11. The threaded connection between the suction cups 12 and the anti-slip base 11 allows the suction cups 12 to be easily replaced or adjusted to adapt to different laboratory table surfaces with varying materials and roughness.
[0031] The anti-slip base 11 is made of ABS and is about 5mm to 8mm thick. It can not only support the weight of the petri dish body 1 and the reagents inside, but also effectively absorb external vibrations to prevent reagents from spilling or becoming contaminated due to external interference.
[0032] The operation process of this utility model is as follows: Remove the cover 2 from the culture dish body 1 and add an appropriate amount of cell culture medium or other reagents into the culture dish body 1. Then, reinstall the cover 2 onto the culture dish body 1, so that the distribution component 3 is located at the top of the culture dish body 1. Next, the experimenter applies force to the outer ring of the cover 2 by hand, and rotates the cover 2 clockwise or counterclockwise about 3 to 5 times through the good friction provided by the transverse anti-slip protrusions 13. As the cover 2 rotates, the elastic lever 5 in the distribution component 3 rotates accordingly, and the flexible scraper 6 connected to its end moves along the inner wall of the annular groove 10, scraping away the reagent remaining on the inner wall of the culture dish body 1 and redistributing it to the bottom area. At the same time, the guide groove 8 on the conical guide head 7 initially guides the dripped reagent, making it flow evenly to the bottom of the culture dish body 1. The vent holes 9 on the surface of the elastic lever 5 allow air circulation and avoid uneven reagent distribution caused by airflow blockage. Throughout the operation, the anti-slip base 11 and suction cup 12 work together to ensure the device remains stable on the lab bench, preventing reagent spillage or contamination due to accidental collisions. The rotation speed is controlled within the range of 10 to 15 rpm to ensure uniform reagent distribution and avoid excessive air bubbles.
[0033] This invention achieves uniform reagent distribution and efficient utilization. In cell culture experiments, uniform reagent distribution is a key factor in ensuring the accuracy and reliability of experimental results. Traditional manual sample addition methods often suffer from localized accumulation, which can easily lead to experimental errors. This invention, through the design of the distribution component 3, particularly the combination of the elastic lever 5 and the flexible scraper 6, enables uniform reagent distribution and residue removal during rotation, improving experimental efficiency and reducing reagent waste.
[0034] To gain a deeper understanding of the working principle of this invention, the functions and cooperative relationships of its components are described in detail below. The central column 4, as the core support structure of the distribution assembly 3, is fixedly connected to the center of the inner wall of the cover 2, ensuring the stability of the entire distribution assembly 3 during rotation. A conical guide head 7 is fixedly connected to the end of the central column 4 away from the cover 2. Multiple guide grooves 8 are circumferentially formed on the outer ring of the conical guide head 7. The inclination angle and width of the guide grooves 8 effectively guide the flow path of the reagent during dripping, ensuring uniform distribution of the reagent in the initial stage. There are 6 to 8 guide grooves 8 to ensure that the reagent covers the entire bottom area of the culture dish body 1.
[0035] The elastic paddle 5 is fixedly connected to the gap between two adjacent guide grooves 8, and is evenly arranged along the circumference of the central column 4 to form a complete annular structure. The elastic paddle 5 is made of medical-grade silicone, which has good elasticity and wear resistance, and can deform appropriately during rotation, thereby driving the flexible scraper 6 to move along the inner wall of the culture dish body 1. The elastic paddle 5 has a thickness of 0.8 mm to 1.2 mm, and its surface has multiple vent holes 9 along its length. The diameter of the vent holes 9 is 0.5 mm to 1 mm, and the spacing is 3 mm to 5 mm. The design of the vent holes 9 not only allows air circulation, but also reduces airflow resistance during rotation, ensuring more uniform reagent distribution.
[0036] A flexible scraper 6, made of soft and wear-resistant polyurethane with a thickness of 0.5 mm to 1 mm, is attached to the end of the elastic lever 5. The end of the flexible scraper 6 contacts an annular groove 10 on the inner wall of the culture dish body 1. The annular groove 10 has a depth of 1 mm to 2 mm and a width of 3 mm to 5 mm. The design of the annular groove 10 ensures a tight fit between the flexible scraper 6 and the inner wall without affecting the overall structural strength of the culture dish body 1. When the lid 2 rotates, the flexible scraper 6 slides along the inner wall of the annular groove 10, precisely scraping away residual reagents and reintroducing them into the bottom area of the culture dish body 1. This design not only reduces reagent waste but also avoids the risk of deterioration or contamination due to prolonged exposure of residual reagents. The end of the flexible scraper 6 can be designed as a wedge or an arc to further optimize the scraping effect and reduce movement resistance.
[0037] The outer ring of the cover 2 is provided with multiple horizontal anti-slip protrusions 13, which increases the friction when holding the hand and prevents accidental slippage.
[0038] This invention is reasonably designed and easy to operate. It performs well in terms of uniform reagent distribution, reducing reagent waste and improving device stability. It can meet the needs of CCK8 experiments and has high practicality and promotion value.
[0039] The present invention can be well realized by following the above embodiments.
Claims
1. A cell culture device for biological experiments, characterized by comprising: The petri dish includes a main body (1) and a cover (2) detachably connected to its top. The bottom of the cover (2) is equipped with a distribution component (3) for uniformly distributing reagents. The distribution component (3) includes a central column (4) fixedly connected to the inner wall of the cover (2). The outer ring of the central column (4) is arranged with multiple elastic paddles (5) in a circumferential array. Each elastic paddle (5) is connected to a flexible scraper (6) at its end. The bottom of the petri dish (1) is provided with an anti-slip base (11).
2. The cell culture device for biological experiments according to claim 1, wherein The central column (4) is fixedly connected to a conical guide head (7) at one end away from the cover (2), and the outer ring of the conical guide head (7) is provided with multiple guide grooves (8) along its circumference.
3. The cell culture device for biological experiments according to claim 2, wherein The elastic paddle (5) is fixedly connected at the gap between two adjacent guide grooves (8).
4. The cell culture device for biological experiments according to any one of claims 1 to 3, characterized in that, The surface of the elastic paddle (5) has multiple ventilation holes (9) along its length.
5. The cell culture device for biological experiments according to claim 4, wherein The inner wall of the culture dish body (1) is provided with an annular groove (10), and the end of the flexible scraper (6) contacts the inner wall of the annular groove (10).
6. The cell culture device for biological experiments according to claim 5, wherein The bottom of the anti-slip base (11) is connected to a plurality of suction cups (12), and the suction cups (12) are connected to the anti-slip base (11) by threads.
7. The cell culture device for biological experiments according to claim 6, wherein The main body (1) of the culture dish is made of transparent material.
8. The cell culture device for biological experiments according to claim 7, wherein The outer ring of the cover (2) is provided with multiple transverse anti-slip protrusions (13).