An electrophoresis analyzer for DNA / RNA quality control

Abstract

This invention discloses an electrophoresis analyzer for DNA / RNA quality control, relating to the field of electrophoresis analysis technology. The invention includes an analysis chamber and an ultraviolet imager fixedly connected to the inner wall of the top of the analysis chamber. It also includes: a limiting part mounted on the analysis chamber; a pop-out part disposed on the analysis chamber; wherein the pop-out part extends into the limiting part, and the limiting part includes a placement component mounted on the analysis chamber; and a stabilizing component disposed on the placement component; wherein the stabilizing component and the placement component are connected. By incorporating the limiting part, this invention solves the problem of difficulty in handling subtle vibrations and fluid disturbances generated by the electric field during electrophoresis, which can easily lead to sample position shifts. This not only causes disordered migration paths of nucleic acid fragments, resulting in detection errors such as molecular weight calculation deviations and concentration misjudgments, but also causes liquid splashing due to sample plate shaking, leading to cross-contamination between wells.

Description

Technical Field

[0001] This invention belongs to the field of electrophoresis analysis technology, and in particular relates to an electrophoresis analyzer for DNA / RNA quality control. Background Technology

[0002] In the medical industry, the quality testing of DNA and RNA is the foundation for downstream experiments such as gene sequencing, molecular cloning, and disease diagnosis. Electrophoresis analysis technology, with its advantages of efficient separation of nucleic acid fragments and accurate determination of molecular weight and concentration, has become a core means of quality inspection. However, traditional electrophoresis analyzers have many technical bottlenecks: on the one hand, the sample fixation method relies on slots or simple clamps, which are easily displaced by electric field forces and vibration interference during electrophoresis, resulting in abnormal fragment migration and signal acquisition deviation, thus producing detection errors and cross-contamination. On the other hand, the sample loading and unloading process is cumbersome, and manual operation is not only inefficient but may also introduce human error and contamination risks. In addition, some instruments use a single detection technology, which is difficult to meet the multi-dimensional analysis needs of complex samples.

[0003] However, existing electrophoresis analyzers are unable to cope with the subtle vibrations and fluid disturbances caused by the electric field during electrophoresis, which can easily lead to sample position shifts. This not only causes disorder in the migration path of nucleic acid fragments, resulting in detection errors such as molecular weight calculation deviations and concentration misjudgments, but also causes liquid splashing due to sample plate shaking, leading to cross-contamination between wells. Utility Model Content

[0004] The purpose of this invention is to provide an electrophoresis analyzer for DNA / RNA quality control. By setting a limiting part, it solves the problem of being unable to cope with the subtle vibrations and fluid disturbances generated by the electric field during electrophoresis, which can easily lead to sample position shift. This not only causes disorder in the migration path of nucleic acid fragments, resulting in detection errors such as molecular weight calculation deviation and concentration misjudgment, but also causes liquid splashing due to sample plate shaking, resulting in cross-contamination between wells.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0006] This utility model is an electrophoresis analyzer for DNA / RNA quality control, including an analysis chamber and an ultraviolet imager fixedly connected to the inner wall of the top of the analysis chamber. It also includes: a limiting part, which is installed on the analysis chamber; and a pop-out part, which is disposed on the analysis chamber; wherein the pop-out part extends into the limiting part.

[0007] Furthermore, the limiting part includes a placement component mounted on the analysis box; and a stabilizing component disposed on the placement component; wherein the stabilizing component and the placement component are connected.

[0008] Furthermore, the pop-out portion includes a limiting component mounted on the analysis box; and an ejection component disposed on the analysis box; wherein the limiting component is located behind the placement component.

[0009] Furthermore, the placement assembly includes a placement box slidably connected to the inner wall of the analysis chamber. A placement plate is fixedly connected to the bottom inner wall of the placement box. A placement groove is formed on the placement plate. An exhaust pipe is provided on the placement plate. The exhaust pipe extends into the placement groove and out of the placement plate. A rubber adsorption pad is fixedly connected to the top of the exhaust pipe. The rubber adsorption pad is located at the central axis of the placement groove, and the exhaust pipe is arranged inside the placement plate.

[0010] Furthermore, the stabilizing component includes a gas collecting pipe connected to the exhaust pipe, a push plate slidably connected to the inner wall of the gas collecting pipe, a ratchet fixedly connected to the front side of the push plate, and a limiting member provided on the gas collecting pipe; wherein, the ratchet is provided with a plurality of ratchet blocks arranged in a linear array on the ratchet, the limiting member includes a limiting block one provided on the ratchet, the limiting block one being adapted to the ratchet, a sliding rod one passing through the gas collecting pipe, the sliding rod one being fixedly connected to the limiting block one, a connecting plate fixedly connected to the right side of the sliding rod one, a spring one sleeved on the outer wall of the sliding rod one, the left side of the spring one being fixedly connected to the gas collecting pipe, and the right side of the spring one being fixedly connected to the connecting plate; wherein, the limiting block one is triangular and adapted to the ratchet blocks on the ratchet.

[0011] Furthermore, the limiting component includes a slide rod two fixedly connected to the inner rear wall of the analyzer, a semi-circular block one fixedly connected to the front side of the slide rod two, a semi-circular block two disposed on the slide rod two, the slide rod two passing through the semi-circular block two, the semi-circular block two slidably connected to the slide rod two, a spring two sleeved on the outer wall of the slide rod two, the front side of the spring two fixedly connected to the semi-circular block two, and the rear side of the spring two fixedly connected to the analyzer; wherein, both the semi-circular block one and the semi-circular block two are semi-circular blocks.

[0012] Furthermore, the ejection assembly includes two spring slide rods disposed within the placement box. Each of the two spring slide rods has a limiting block two on its side closest to each other. Two pushing members are disposed on the rear side of the placement box. Each spring slide rod includes a slide rod and a spring, with the spring sleeved on the slide rod and the slide rod passing through the limiting block two. Each pushing member includes a damper fixedly connected to the inner rear wall of the analysis chamber. The front side of the damper contacts the placement box. A spring three is sleeved on the outer wall of the damper. The rear side of the spring three is fixedly connected to the analysis chamber, and the front side of the spring three contacts the placement box. The two pushing members are arranged in a mirror image.

[0013] This utility model has the following beneficial effects:

[0014] 1. By setting a limiting part, the DNA or RNA quality inspection sample after electrophoresis needs to be placed in the placement box. First, align the sample with the placement slot on the placement plate, then place it on the placement slot and make contact with the rubber adsorption pad. Then press down, at which point the rubber adsorption pad will be squeezed downwards. After squeezing, the air inside the rubber adsorption pad will be squeezed into the exhaust pipe. At this time, the air in the exhaust pipe will flow into the gas collection pipe through the pipe. At this time, the push plate in the gas collection pipe will be pushed forward by the air pressure. When the push plate is pushed, the ratchet will also move forward. When the ratchet moves forward, the limiting block 1 will be pushed to the left. Slide bar 1 will also move accordingly. When slide bar 1 moves, the connecting plate will pull spring 1 accordingly. As slide bar 1 moves, limit block 1 can smoothly pass through the toothed block on the ratchet. Due to the angle of limit block 1, limit block 1 will not slide in the opposite direction after passing through the ratchet. After the air in the rubber adsorption pad is discharged, to prevent the sample displacement caused by insufficient pressure, the ratchet can be manually pulled to make the push plate slide in the gas collecting tube, thereby increasing the gas pressure in the exhaust tube, thus increasing the adsorption force on the sample, ensuring that the sample remains stable during electrophoresis analysis, avoiding detection errors, cross-contamination or instrument damage caused by displacement, and effectively improving the accuracy and repeatability of the detection results.

[0015] 2. By setting a pop-out section, after the sample is placed, the placement box is pushed into the analysis chamber. During the push, the second limiting block on the placement box will first contact the first semicircular block. After the first semicircular block contacts the second limiting block, with continued pushing, the second limiting block will slide on the slide rod of the spring slide rod. During the sliding, the second limiting block will compress the spring on the spring slide rod. As it is compressed, the second limiting block will contract until it passes the first semicircular block. After passing the first semicircular block, the placement box is restricted. After the sample on the placement box is fixed, the ultraviolet imager is started. After the detection is completed, the placement box is pushed inward again, so that the second limiting block passes the second semicircular block on the slide rod. With the push of the placement box, the damper and spring... The third spring will be compressed and then released from the placement box. At this time, the damper and the third spring will push the placement box outward. The second limiting block on the placement box will move outward with the second semicircular block. As the second semicircular block moves, it will come into contact with the first semicircular block. After contact, the first and second semicircular blocks will form a circle. At this time, the third spring and the damper will continue to push the second limiting block to contract through the first and second semicircular blocks, thus achieving the effect of pushing the placement box out. This achieves a seamless connection of the entire process from sample loading, testing to removal, without the need for manual disassembly of parts. It can avoid detection deviations caused by installation errors and simplify operation through an automated push-pull mechanism, reduce human contact interference, and make the testing process more efficient and safe.

[0016] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a partial cross-sectional view of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 3 This is a partial cross-sectional view of the limiting part of this utility model;

[0021] Figure 4 This is a partial cross-sectional view of the pop-out section of this utility model;

[0022] Figure 5 This utility model Figure 3 A magnified structural diagram of A in the middle;

[0023] Figure 6 This utility model Figure 1 A magnified structural diagram of B in the diagram.

[0024] The attached diagram lists the components represented by each number as follows:

[0025] 111. Analyzer; 112. Ultraviolet Imager; 2. Limiting Part; 21. Placement Assembly; 211. Placement Box; 212. Placement Plate; 213. Placement Slot; 214. Exhaust Pipe; 215. Rubber Adsorption Pad; 22. Stabilizing Assembly; 221. Gas Collection Pipe; 222. Push Plate; 223. Ratchet; 224. Limiting Block 1; 225. Slide Rod 1; 226. Connecting Plate; 227. Spring 1; 3. Pop-out Part; 31. Limiting Assembly; 311. Slide Rod 2; 312. Semicircular Block 1; 313. Semicircular Block 2; 314. Spring 2; 32. Push-out Assembly; 321. Spring Slide Rod; 322. Limiting Block 2; 323. Damper; 324. Spring 3. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Please see Figure 1-6 As shown, this utility model is an electrophoresis analyzer for DNA / RNA quality control, including an analysis chamber 111 and an ultraviolet imager 112 fixedly connected to the inner wall of the top of the analysis chamber 111. It also includes: a limiting part 2, mounted on the analysis chamber 111; and a pop-out part 3, disposed on the analysis chamber 111; wherein the pop-out part 3 extends into the limiting part 2, the limiting part 2 including a placement component 21, mounted on the analysis chamber 111; and a stabilizing component 22, disposed on the placement component 21. The placement assembly 21 is connected to the placement component 21, which includes a placement box 211 slidably connected to the inner wall of the analysis chamber 111. A placement plate 212 is fixedly connected to the bottom inner wall of the placement box 211. A placement groove 213 is provided on the placement plate 212. An exhaust pipe 214 is provided on the placement plate 212, extending into the placement groove 213 and out of the placement plate 212. A rubber adsorption pad 215 is fixedly connected to the top of the exhaust pipe 214. The rubber adsorption pad 215 is located at the central axis of the placement groove 213, and the exhaust pipe 214 is arranged in the placement groove 213. Inside plate 212, stabilizing component 22 includes an air collecting pipe 221 connected to exhaust pipe 214. A push plate 222 is slidably connected to the inner wall of the air collecting pipe 221. A ratchet 223 is fixedly connected to the front side of the push plate 222. A limiting member is provided on the air collecting pipe 221. The ratchet 223 has several ratchet blocks arranged in a linear array. The limiting member includes a limiting block 224 on the ratchet 223, which is adapted to the ratchet 223. A sliding rod 225 passes through the air collecting pipe 221, and the sliding rod 225 is connected to the limiting block 224. Block 224 is fixedly connected, and a connecting plate 226 is fixedly connected to the right side of slide rod 225. Spring 227 is sleeved on the outer wall of slide rod 225. The left side of spring 227 is fixedly connected to gas collecting pipe 221, and the right side of spring 227 is fixedly connected to connecting plate 226. The limiting block 224 is triangular and matches the ratchet on ratchet 223. By setting the limiting part 2, the sample is kept stable during electrophoresis analysis, avoiding detection errors, cross-contamination or instrument damage caused by displacement, and effectively improving the accuracy and repeatability of the detection results.

[0028] The ejection part 3 includes a limiting component 31, which is mounted on the analysis box 111; and an ejection component 32, which is disposed on the analysis box 111. The limiting component 31 is located behind the placement component 21 and includes a second slide rod 311 fixedly connected to the inner rear wall of the analysis box 111. A semicircular block 312 is fixedly connected to the front of the slide rod 311, and a semicircular block 313 is disposed on the slide rod 311. The slide rod 311 passes through the semicircular block 313, and the semicircular block 313 is slidably connected to the slide rod 311. A second spring 314 is sleeved on the outer wall of the slide rod 311. The front of the spring 314 is fixedly connected to the semicircular block 313, and the rear of the spring 314 is fixedly connected to the analysis box 111. Both the semicircular block 312 and the semicircular block 313 are semicircular. The ejection component 32 includes two spring slide rods 321 disposed within the placement box 211. Each spring slide bar 321 has a limiting block 322 on one side that is close to each other, and two pushers are provided on the rear side of the placement box 211. The spring slide bar 321 includes a slide bar and a spring, with the spring sleeved on the slide bar and the slide bar passing through the limiting block 322. The pushers include a damper 323 fixedly connected to the inner rear wall of the analysis box 111. The front side of the damper 323 contacts the placement box 211, and a spring 324 is sleeved on the outer wall of the damper 323. The rear side of the spring 324 is fixedly connected to the analysis box 111, and the front side of the spring 324 contacts the placement box 211. The two pushers are arranged in a mirror image. By setting the pop-out part 3, a seamless connection is achieved in the entire process of sample loading, detection and removal. There is no need to manually disassemble parts. This can avoid detection deviation caused by installation error, and the automated push-pull mechanism simplifies operation, reduces human contact interference, and makes the detection process more efficient and safe.

[0029] One specific application of this embodiment is as follows: During use, the electrophoresis-treated DNA or RNA quality control sample needs to be placed in the placement box 211. First, align the sample with the placement slot 213 on the placement plate 212, then place it on the placement slot 213 and make contact with the rubber adsorption pad 215. Then, press down. At this time, the rubber adsorption pad 215 will be squeezed downwards. After squeezing, the air inside the rubber adsorption pad 215 will be squeezed into the exhaust pipe 214. The air in the exhaust pipe 214 will then flow through the pipe into the gas collecting pipe 221. At this time, the push plate 222 in the gas collecting pipe 221 will be pushed forward by the air pressure. When the push plate 222 is pushed, the ratchet 223 will also move forward. As the ratchet 223 moves forward... Limiting block 224 is pushed to the left. As limiting block 224 is pushed to the left, sliding rod 225 also moves accordingly. When sliding rod 225 moves, connecting plate 226 pulls spring 227. With the movement of sliding rod 225, limiting block 224 can smoothly pass through the teeth on ratchet 223. Due to the angle of limiting block 224, it will not slide backward after passing ratchet 223. After the air in the rubber adsorption pad 215 is expelled, to prevent sample displacement due to insufficient pressure, ratchet 223 can be manually pulled to slide push plate 222 within gas collecting pipe 221, thereby increasing the air pressure in exhaust pipe 214 and thus increasing the adsorption force on the sample. After the sample is placed, place box 211... The sample is pushed into the analysis box 111. During this process, the second limiting block 322 on the placement box 211 first contacts the first semicircular block 312. After the first semicircular block 312 contacts the second limiting block 322, with continued pushing, the second limiting block 322 slides on the slide bar on the spring slide bar 321. During this sliding, the second limiting block 322 compresses the spring on the spring slide bar 321. As it compresses, the second limiting block 322 contracts until it passes the first semicircular block 312. After passing the first semicircular block 312, the placement box 211 is effectively restricted. After the sample on the placement box 211 is fixed, the ultraviolet imager 112 is activated. The ultraviolet imager 112 here is a LUV-270A. The high-intensity darkroom UV analyzer developed and manufactured by Yang Instruments Co., Ltd. can be used to observe and photograph DNA and RNA electrophoresis gel samples and to detect proteins and nucleotides. It uses a sealed, high-quality ABS plastic integrated darkroom, eliminating the need for operation in a dark room. It has UV gel cutting devices on both sides, a UV-protected observation window, and a specially treated UV filter for a clear background without lamp shadows. It also includes a fixed camera port and frame, compatible with most SLR cameras on the market. After detection, pushing the placement box 211 inward again causes the limiting block 322 to pass through the semi-circular block 313 on the slide rod 311. As the placement box 211 is pushed, the damper 323 and spring 324 are compressed, and then the placement box 211 is released.At this time, the damper 323 and spring 324 push the placement box 211 outward. The limiting block 322 on the placement box 211 moves outward along with the semicircular block 313. As the semicircular block 313 moves, it comes into contact with the first semicircular block 312. After contact, the first and second semicircular blocks form a circle. The spring 324 and damper 323 continue to push the limiting block 322, causing it to contract and pass through the semicircular blocks 312 and 313, thus pushing the placement box 211 outward.

[0030] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0031] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. An electrophoresis analyzer for DNA / RNA quality control, comprising an analysis chamber (111) and an ultraviolet imager (112) fixedly connected to the inner wall of the top of the analysis chamber (111), characterized in that, Also includes: A limiting part (2) is installed on the analysis box (111); as well as Pop-out section (3), said pop-out section (3) is disposed on the analysis box (111); The pop-out part (3) extends into the limiting part (2).

2. The electrophoresis analyzer for DNA / RNA quality control according to claim 1, characterized in that, The limiting part (2) includes a placement component (21) mounted on the analysis box (111); and A stabilizing component (22) is disposed on the placement component (21); The stabilizing component (22) and the placement component (21) are connected.

3. The electrophoresis analyzer for DNA / RNA quality control according to claim 2, characterized in that, The pop-out portion (3) includes a limiting component (31) mounted on the analysis box (111); and An ejection component (32) is disposed on the analysis box (111); The limiting component (31) is located behind the placement component (21).

4. The electrophoresis analyzer for DNA / RNA quality control according to claim 3, characterized in that, The placement assembly (21) includes a placement box (211) slidably connected to the inner wall of the analysis box (111). A placement plate (212) is fixedly connected to the bottom inner wall of the placement box (211). A placement groove (213) is provided on the placement plate (212). An exhaust pipe (214) is provided on the placement plate (212). The exhaust pipe (214) extends into the placement groove (213) and extends out of the placement plate (212). A rubber adsorption pad (215) is fixedly connected to the top of the exhaust pipe (214). Among them, the rubber adsorption pad (215) is located at the central axis of the placement groove (213), and the exhaust pipe (214) is arranged in the placement plate (212).

5. The electrophoresis analyzer for DNA / RNA quality control according to claim 4, characterized in that, The stabilizing component (22) includes a gas collecting pipe (221) connected to the exhaust pipe (214), a push plate (222) is slidably connected to the inner wall of the gas collecting pipe (221), a ratchet (223) is fixedly connected to the front side of the push plate (222), and a limiting member is provided on the gas collecting pipe (221); Among them, a number of thorn blocks are provided on the thorn (223), and the thorn blocks are arranged in a linear array on the thorn (223).

6. The electrophoresis analyzer for DNA / RNA quality control according to claim 5, characterized in that, The limiting component (31) includes a slide rod two (311) fixedly connected to the inner wall of the rear side of the analyzer (111). A semicircular block one (312) is fixedly connected to the front side of the slide rod two (311). A semicircular block two (313) is provided on the slide rod two (311). The slide rod two (311) passes through the semicircular block two (313). The semicircular block two (313) is slidably connected to the slide rod two (311). A spring two (314) is sleeved on the outer wall of the slide rod two (311). The front side of the spring two (314) is fixedly connected to the semicircular block two (313). The rear side of the spring two (314) is fixedly connected to the analyzer (111). Among them, semicircular block one (312) and semicircular block two (313) are both semicircular blocks.

7. The electrophoresis analyzer for DNA / RNA quality control according to claim 6, characterized in that, The ejection assembly (32) includes two spring slide rods (321) disposed in the placement box (211). Each of the two spring slide rods (321) is provided with a limit block (322) on the side that is close to each other. Two pushers are provided on the rear side of the placement box (211). Among them, the spring slide rod (321) includes a slide rod and a spring, the spring is sleeved on the slide rod, and the slide rod passes through the limiting block two (322).

8. The electrophoresis analyzer for DNA / RNA quality control according to claim 7, characterized in that, The limiting component includes a limiting block (224) disposed on the ratchet (223), the limiting block (224) being adapted to the ratchet (223), a sliding rod (225) passing through the air collecting pipe (221), the sliding rod (225) being fixedly connected to the limiting block (224), a connecting plate (226) being fixedly connected to the right side of the sliding rod (225), a spring (227) being sleeved on the outer wall of the sliding rod (225), the left side of the spring (227) being fixedly connected to the air collecting pipe (221), and the right side of the spring (227) being fixedly connected to the connecting plate (226); Among them, the limiting block 1 (224) is a triangular block and is adapted to the ratchet block on the ratchet (223).

9. The electrophoresis analyzer for DNA / RNA quality control according to claim 8, characterized in that, The pusher includes a damper (323) fixedly connected to the inner wall of the rear side of the analyzer (111). The front side of the damper (323) is in contact with the placement box (211). A spring (324) is sleeved on the outer wall of the damper (323). The rear side of the spring (324) is fixedly connected to the analyzer (111), and the front side of the spring (324) is in contact with the placement box (211). The two actuators are set in a mirror image.

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