Apparatus for producing canine feline interferon complex peptide
By designing a centrifuge device that includes components such as a base, motor, and disc, centrifugal force is used to automatically separate solid impurities from cell fluid, solving the problems of low separation efficiency and high contamination risk in existing technologies, and achieving efficient and stable solid-liquid separation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HARBIN TAIYUAN BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-06-16
- Publication Date
- 2026-05-29
AI Technical Summary
Existing centrifugation devices are difficult to effectively separate solid impurities such as cell debris and organelles from target complex peptides when processing canine and feline cell fluids, resulting in complex manual operation and easy contamination.
A preparation device comprising a base, motor, disc, limiting port, through port, centrifuge tube, insert, top cover, and valves was designed. It automatically separates solid impurities in cell fluid using centrifugal force and achieves solid-liquid separation through the automatic opening and closing of the valves, simplifying operation and improving separation efficiency.
This method enables rapid and stable separation of solid impurities from cell fluid, yielding high-purity interfering viral complex peptide solutions while reducing the complexity of manual operations and the risk of contamination.
Smart Images
Figure CN224293539U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of preparation device technology, and particularly relates to a preparation device for producing interfering viral complex peptides in dogs and cats. Background Technology
[0002] Canine and feline interferon complex peptides are artificially designed synthetic or recombinant peptides that mimic or enhance the antiviral effects of animal interferon. They are used to combat viral infections in dogs and cats. The preparation equipment for canine and feline interferon complex peptides includes bioreactors, filtration equipment, and centrifugation equipment.
[0003] Existing centrifugation devices, when processing canine and feline cell lysates to obtain interfering viral complex peptides, can achieve preliminary solid-liquid separation in the cell lysate using centrifugal force, causing the precipitate (containing cell debris, organelles, and other solid impurities) to separate from the supernatant containing the target complex peptide in the centrifuge tube. However, the precipitated cell debris, organelles, and other solid impurities still coexist with the supernatant containing the target complex peptide in the centrifuge tube, requiring secondary transfer and separation by manual pouring or siphoning, which is time-consuming, labor-intensive, and prone to recontamination. Therefore, improvements are needed. To address this, a device for preparing interfering viral complex peptides from canines and cats is proposed. Utility Model Content
[0004] The purpose of this invention is to provide a device for preparing interfering viral complex peptides produced by dogs and cats, so as to solve the problems mentioned in the background art.
[0005] To achieve the above objectives, the present invention adopts the following technical solution: a device for preparing interfering viral complex peptides produced by dogs and cats, comprising a base, a motor fixedly installed in the middle of the base, a disc fixedly installed at the end of the motor output shaft, a uniformly distributed limiting port on the side of the disc, and a through port on each side of the limiting port. The side of the disc, the limiting port, and the through port are all inclined. A centrifuge tube is provided on the through port. The centrifuge tube is a through-type tube. An insert is fixedly connected to the top of the centrifuge tube. The size of the insert is adapted to the size of the limiting port. A top cover is threadedly connected to the top of the centrifuge tube. A valve one is provided at the bottom of the centrifuge tube. A bottom cover is threadedly connected to the bottom of the centrifuge tube. A valve two is provided at the opening of the bottom cover.
[0006] As a further description of the above solution: by setting up a base, motor, disc, limiting port, through port, centrifuge tube, insert block, top cover, valve one, bottom cover, and valve two working together, it is possible to quickly and directly separate canine and feline cell fluid into solid and liquid components. Centrifugal force is used to quickly cause cell debris, organelles and other solid impurities to settle and be isolated, efficiently obtaining a clear solution containing the target interfering viral complex peptide, which is highly practical.
[0007] Preferably, each of the limiting ports is provided with a rubber frame, and a damping mechanism is provided between the insert block and the rubber frame.
[0008] As a further description of the above scheme: each limiting port is equipped with a rubber frame, and damping is provided between the insertion block and the rubber frame, which can improve the stability of the centrifugation process of the centrifuge tube.
[0009] Preferably, the size of the centrifuge tube is adapted to the size of the through-hole.
[0010] As a further description of the above solution: the size of the centrifuge tube is matched with the size of the through-hole, which can further improve the stability of the centrifugation process.
[0011] Preferably, both the centrifuge tube and the side of the bottom cover are provided with graduations.
[0012] As a further description of the above scheme: both the centrifuge tubes and the bottom caps are equipped with graduations on their sides, which makes it easy to replace centrifuge tubes and bottom caps of different heights according to the volume of canine and feline cell fluid.
[0013] Preferably, the base is provided with a timer and a controller, and the controller is electrically connected to the timer and the motor respectively.
[0014] As a further description of the above solution: a timer and a controller are installed on the base. The controller is electrically connected to the timer and the motor respectively, which facilitates the control of the centrifugation time of the canine and cat cell solution.
[0015] In summary, compared with the prior art, the beneficial effects of this utility model are: by setting up a base, motor, disc, limiting port, through port, centrifuge tube, insert block, top cover, valve one, bottom cover, and valve two working together, it can quickly and directly separate canine and cat cell fluid into solid and liquid components, and use centrifugal force to quickly cause cell debris, organelles and other solid impurities to settle and be isolated, efficiently obtaining a clear solution containing target interfering viral complex peptides, which is highly practical. Attached Figure Description
[0016] Figure 1 This is a three-dimensional structural diagram of the present invention;
[0017] Figure 2 This is a structural diagram of the disk body of this utility model;
[0018] Figure 3 This is a structural diagram of the centrifuge tube, insert, top cover, and bottom cover of this utility model;
[0019] Figure 4 This is a structural diagram of the centrifuge tube and valve of this utility model;
[0020] Figure 5 This is a structural diagram of the bottom cover and valve of this utility model.
[0021] Legend:
[0022] 1. Base; 2. Motor; 3. Disc; 4. Limiting port; 5. Through port; 6. Centrifuge tube; 7. Insert block; 8. Top cover; 9. Valve 1; 10. Bottom cover; 11. Valve 2. Detailed Implementation
[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.
[0024] Please see Figure 1-5 This utility model provides a technical solution:
[0025] A device for preparing interfering viral complex peptides produced by dogs and cats includes a base 1, a motor 2 fixedly installed in the middle of the base 1, a disc 3 fixedly installed at the end of the output shaft of the motor 2, a uniformly distributed limiting port 4 on the side of the disc 3, and a through port 5 on each side of the disc 3 at the limiting port 4. The side of the disc 3, the limiting port 4, and the through port 5 are all set in an inclined shape. A centrifuge tube 6 is set on the through port 5. The centrifuge tube 6 is set in a through-type configuration. An insert 7 is fixedly connected to the top of the centrifuge tube 6. The size of the insert 7 is adapted to the size of the limiting port 4. A top cover 8 is threadedly connected to the top of the centrifuge tube 6. A valve 9 is set at the bottom of the centrifuge tube 6. A bottom cover 10 is threadedly connected to the bottom of the centrifuge tube 6. A valve 11 is set at the opening of the bottom cover 10.
[0026] When in use, the base 1 is used to support the entire device;
[0027] When performing solid-liquid separation of canine and feline cell fluid, the operator injects the canine and feline cell fluid into centrifuge tube 6. Valve 9 seals the bottom of centrifuge tube 6 to prevent leakage of canine and feline cell fluid. Then, the top cover 8 is threaded to the top of centrifuge tube 6 to seal its top. Centrifuge tube 6 is then passed through the through-hole 5 of disc 3, and the insert 7 is inserted into the limiting port 4. After that, the bottom cover 10 is threaded to the bottom of centrifuge tube 6. When the bottom cover 10 is connected in place, valve 9 and valve 11 fit together perfectly.
[0028] The motor 2 in the middle of the base 1 is started. The motor 2 drives the disc 3 to rotate at high speed. Centrifugal force is used to make solid impurities such as cell debris and organelles in the canine and cat cell fluid settle quickly. During the settling of solid impurities, valve 1 9 and valve 2 11 are automatically opened by force. After opening, the solid impurities are deposited in the bottom cover 10 during centrifugation. The clear solution containing the target interfering virus complex peptide remains in the centrifuge tube 6, realizing solid-liquid separation. When centrifugation is completed, valve 1 9 and valve 2 11 automatically close under their own elasticity, effectively isolating the precipitated impurities in the bottom cover 10 and preventing them from mixing back into the supernatant in the centrifuge tube 6.
[0029] Then remove the bottom cover 10 from the bottom of the centrifuge tube 6, and the centrifuge tube 6 can be taken out from the plate 3. After taking it out, the clear solution containing the target interfering viral complex peptide in the centrifuge tube 6 can be taken directly.
[0030] This device for preparing interfering viral complex peptides in dogs and cats can quickly and directly separate solid and liquid components from canine and feline cells. It uses centrifugal force to rapidly settle and isolate solid impurities such as cell debris and organelles, efficiently obtaining a clear solution containing the target interfering viral complex peptide, making it highly practical.
[0031] Each limiting port 4 is provided with a rubber frame, and the insertion block 7 is provided with damping between the rubber frame and the rubber frame;
[0032] Each limiting port 4 is equipped with a rubber frame, and the insertion block 7 is damped between the rubber frame and the insertion block 7. When the operator inserts the insertion block 7 of the centrifuge tube 6 into the limiting port 4, the damping force between the rubber frame and the insertion block 7 can effectively reduce the shaking of the centrifuge tube 6 during high-speed rotation, thereby improving the stability of the centrifugation process of the centrifuge tube 6.
[0033] The size of centrifuge tube 6 is compatible with the size of the through-hole 5;
[0034] The size of centrifuge tube 6 is compatible with the size of the through-hole 5, which can further improve the stability of the centrifugation process of centrifuge tube 6.
[0035] Both the centrifuge tubes 6 and the bottom cover 10 have graduations on their sides;
[0036] Both the centrifuge tube 6 and the bottom cover 10 have graduations on their sides. Operators can select the appropriate height of the centrifuge tube 6 and the bottom cover 10 by observing the graduations according to the actual volume of the canine and cat cell solution, which improves the adaptability of the device to different processing volumes.
[0037] A timer and a controller are provided on the base 1, and the controller is electrically connected to the timer and the motor 2 respectively;
[0038] The base 1 is equipped with a timer and a controller. The controller is electrically connected to the timer and the motor 2, respectively, to facilitate the control of the centrifugation time of the canine and cat cell solution.
[0039] Working principle:
[0040] When in use, the base 1 is used to support the entire device;
[0041] When performing solid-liquid separation of canine and feline cell fluid, the operator injects the canine and feline cell fluid into centrifuge tube 6. Valve 9 seals the bottom of centrifuge tube 6 to prevent leakage of canine and feline cell fluid. Then, the top cover 8 is threaded to the top of centrifuge tube 6 to seal its top. Centrifuge tube 6 is then passed through the through-hole 5 of disc 3, and the insert 7 is inserted into the limiting port 4. After that, the bottom cover 10 is threaded to the bottom of centrifuge tube 6. When the bottom cover 10 is connected in place, valve 9 and valve 11 fit together perfectly.
[0042] The motor 2 in the middle of the base 1 is started. The motor 2 drives the disc 3 to rotate at high speed. Centrifugal force is used to make solid impurities such as cell debris and organelles in the canine and cat cell fluid settle quickly. During the settling of solid impurities, valve 1 9 and valve 2 11 are automatically opened by force. After opening, the solid impurities are deposited in the bottom cover 10 during centrifugation. The clear solution containing the target interfering virus complex peptide remains in the centrifuge tube 6, realizing solid-liquid separation. When centrifugation is completed, valve 1 9 and valve 2 11 automatically close under their own elasticity, effectively isolating the precipitated impurities in the bottom cover 10 and preventing them from mixing back into the supernatant in the centrifuge tube 6.
[0043] Then remove the bottom cover 10 from the bottom of the centrifuge tube 6, and the centrifuge tube 6 can be taken out from the plate 3. After taking it out, the clear solution containing the target interfering viral complex peptide in the centrifuge tube 6 can be taken directly.
[0044] This device for preparing interfering viral complex peptides in dogs and cats can quickly and directly separate solid and liquid components from dog and cat cell fluids. It uses centrifugal force to quickly settle and isolate solid impurities such as cell debris and organelles, efficiently obtaining a clear solution containing the target interfering viral complex peptide. It is highly practical.
[0045] in,
[0046] Each limiting port 4 is equipped with a rubber frame, and the insertion block 7 is damped between the rubber frame and the insertion block 7. When the operator inserts the insertion block 7 of the centrifuge tube 6 into the limiting port 4, the damping force between the rubber frame and the insertion block 7 can effectively reduce the shaking of the centrifuge tube 6 during high-speed rotation, thereby improving the stability of the centrifuge tube 6 during the centrifugation process.
[0047] The size of centrifuge tube 6 is compatible with the size of the through-hole 5, which can further improve the stability of the centrifugation process of centrifuge tube 6;
[0048] Both the centrifuge tube 6 and the bottom cover 10 have graduations on their sides. Operators can select the appropriate height of the centrifuge tube 6 and the bottom cover 10 by observing the graduations according to the actual volume of the canine and cat cell solution, which improves the adaptability of the device to different processing volumes.
[0049] The base 1 is equipped with a timer and a controller. The controller is electrically connected to the timer and the motor 2, respectively, to facilitate the control of the centrifugation time of the canine and cat cell solution.
[0050] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.
Claims
1. A device for preparing interfering viral complex peptides produced by dogs and cats, comprising a base (1), characterized in that, A motor (2) is fixedly installed in the middle of the base (1). A disc (3) is fixedly installed at the end of the output shaft of the motor (2). The disc (3) has evenly distributed limiting ports (4) on its side. The disc (3) has through ports (5) on the side of the limiting ports (4). The side of the disc (3), the limiting ports (4), and the through ports (5) are all set in an inclined shape. A centrifuge tube (6) is set on the through port (5). The centrifuge tube (6) is set in a through-type. A plug (7) is fixedly connected to the top of the centrifuge tube (6). The size of the plug (7) is adapted to the size of the limiting port (4). A top cover (8) is threaded to the top of the centrifuge tube (6). A valve one (9) is set at the bottom of the centrifuge tube (6). A bottom cover (10) is threaded to the bottom of the centrifuge tube (6). A valve two (11) is set at the opening of the bottom cover (10).
2. The apparatus for preparing interfering viral complex peptides produced by dogs and cats according to claim 1, characterized in that, Each of the limiting ports (4) is provided with a rubber frame, and the insertion block (7) is provided with damping between the rubber frame and the rubber frame.
3. The apparatus for preparing interfering viral complex peptides produced by dogs and cats according to claim 1, characterized in that, The size of the centrifuge tube (6) is adapted to the size of the through-hole (5).
4. The apparatus for preparing interfering viral complex peptides produced by dogs and cats according to claim 1, characterized in that, The centrifuge tube (6) and the bottom cover (10) are both provided with scales on their sides.
5. The apparatus for preparing interfering viral complex peptides produced by dogs and cats according to claim 1, characterized in that, The base (1) is equipped with a timer and a controller, and the controller is electrically connected to the timer and the motor (2) respectively.