Division-marked petri dish for confrontation culture of actinomyces

Abstract

The utility model belongs to the culture dish technical field, and disclose the partition mark culture dish for actinomycete flat plate confrontation culture, including connecting plate two, the upper end surface of connecting plate two is fixed with the pivot, the outside of pivot is equipped with the magnetic attraction ring, the outside fixed establishment of magnetic attraction ring is equipped with the fixed plate, the lower end surface fixed establishment of fixed plate is equipped with the spring, and the spring is fixedly arranged with connecting plate two, through the magnetic attraction ring, can let fixed plate and connecting plate two quick adhesion or separate, can stabilize and fix the partition mark when adhesion, when separating, it is convenient for inoculating the strain to each partition, and the operation does not need to disassemble the complex component, through the spring, can buffer the contact pressure of fixed plate and flat plate, both avoid crushing the culture medium, and can reset the fixed plate automatically when opening and closing, reduces the manual adjustment step.

Description

Technical Field

[0001] This invention belongs to the field of petri dish technology, specifically a zoned and marked petri dish for confrontation culture of actinomycetes on agar plates. Background Technology

[0002] In the field of actinomycete research, plate confrontation culture is a core experimental method for investigating the interaction between actinomycetes and other microorganisms (such as pathogens and symbiotic bacteria) and screening for antimicrobial active strains. Traditional culture dishes are mostly undivided monolithic structures, requiring manual streaking to divide the experiment, which has problems such as low partitioning accuracy, easy blurring of labels, high risk of cross-contamination between different experimental groups, and large errors in subsequent observation and counting.

[0003] Meanwhile, a culture dish for a biological control plate confrontation experiment is disclosed (announcement number CN206706088U). The culture dish includes a base plate and a side plate that bends and extends from the base plate. The base plate and the side plate enclose a receiving space. The base plate includes an upper surface, at least one scale line extending outward from the center of the upper surface, and a marking portion formed on the scale line.

[0004] The aforementioned culture dish for a biocontrol plate confrontation experiment is prone to damage during transportation and transfer due to the cross lines and marking holes on the surface of glass culture dishes. Furthermore, since glass culture dishes are generally reusable, the presence of cross lines and marking holes reduces their reusability. Therefore, this culture dish can generally only be developed for disposable plastic culture dishes, and its application is not widespread.

[0005] Therefore, in response to the above problems, a zoned labeling culture dish for the confrontation culture of actinomycetes on agar plates is proposed. Utility Model Content

[0006] To address the problems mentioned in the background art, this utility model provides a zoned labeling culture dish for the confrontation culture of actinomycetes, which has the advantages of convenient operation, accurate positioning, good sealing effect, and easy zoned culture.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a partitioned labeling culture dish for actinomycete plate confrontation culture, comprising a second connecting plate, a rotating shaft fixedly mounted on the upper end face of the second connecting plate, a magnetic ring mounted on the outer side of the rotating shaft, a fixing plate fixedly mounted on the outer side of the magnetic ring, and a spring fixedly mounted on the lower end face of the fixing plate, and the spring being fixedly mounted to the second connecting plate. The spring can buffer the contact pressure between the fixing plate and the plate, thus avoiding crushing the culture medium and automatically resetting the fixing plate when opening and closing, reducing manual adjustment steps.

[0008] Preferably, a magnetic column is attached to the inner side of the magnetic ring, a connecting plate is fixedly provided on the upper end face of the magnetic column, and a partition plate is fixedly provided on the outer side of the magnetic column.

[0009] By adopting the above technical solution, the culture dish has a partitioning function through the first partition plate, which can separate different actinomycete strains and avoid mutual interference.

[0010] Preferably, the partition plate has a square groove inside, and the magnetic column is inside the square groove.

[0011] By adopting the above technical solution, the magnetic column is embedded inside the partition plate through the square groove, instead of the traditional external structure of "magnetic column directly fixing the partition plate to the outside".

[0012] Preferably, a partition plate 2 is fixedly provided on the outer side of the connecting plate 2, a slot 1 is provided inside the partition plate 2, and a positioning block is fixedly provided on the outer side of the partition plate 2.

[0013] By adopting the above technical solution, the partitioning function of the petri dish is further enhanced by the second partition plate, and the design of the first slot and the positioning block provides convenience for the assembly and positioning of the petri dish.

[0014] Preferably, a limiting plate is fixedly provided on the lower end face of the first partition plate, a sealing plate is fixedly provided on the lower end face of the limiting plate, and a second slot is provided inside the first partition plate, and the second slot is fitted to the first slot.

[0015] By adopting the above technical solution, the sealing and stability of the petri dish are enhanced through the use of limiting plates and sealing plates.

[0016] Preferably, a culture dish body is fixedly provided on the outside of the sealing plate, and a limiting groove is formed inside the culture dish body, and the limiting groove is fitted to the limiting plate.

[0017] By adopting the above technical solution, the position of the separator plate can be kept stable in the petri dish through the limiting groove and limiting plate, thereby providing a stable environment for the culture of actinomycetes.

[0018] Preferably, the interior of the culture dish body is provided with a positioning groove, and the positioning groove is fitted to the positioning block.

[0019] By adopting the above technical solution, the positioning groove and positioning block enable the various components of the petri dish to be tightly combined to form a complete whole, thereby improving the overall performance of the petri dish.

[0020] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0021] 1. This utility model uses a magnetic suction ring to allow the fixing plate and the connecting plate to be quickly attached or separated. When attached, it can stably fix the partition markings, and when separated, it is convenient to inoculate the bacterial strains into each partition. The operation does not require disassembling complex parts. The spring can buffer the contact pressure between the fixing plate and the plate, which can not only avoid crushing the culture medium, but also automatically reset the fixing plate when opening and closing, reducing the number of manual adjustment steps.

[0022] 2. This utility model enables the various components of the petri dish to be accurately assembled together through the first slot and the positioning block, thereby improving the efficiency and stability of the petri dish. Through the second slot, the fitting arrangement of the second slot with the first slot further strengthens the connection between the first partition plate and the second partition plate, making the partitioning of the petri dish more secure. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0024] Figure 2 This is a schematic diagram of the installation structure of the limiting groove of this utility model;

[0025] Figure 3 This is a schematic diagram of the installation structure of the partition plate of this utility model;

[0026] Figure 4 This is a schematic diagram of the secondary installation structure of the partition plate of this utility model;

[0027] Figure 5 This utility model Figure 4 Enlarged structural diagram at point A in the middle.

[0028] Figure 6 This is a schematic diagram of the installation structure of the card slot one of this utility model.

[0029] In the diagram: 1. Petri dish body; 2. Partition plate one; 3. Partition plate two; 4. Positioning block; 5. Positioning groove; 6. Limiting groove; 7. Connecting plate one; 8. Magnetic column; 9. Square groove; 10. Limiting plate; 11. Sealing plate; 12. Slot one; 13. Slot two; 14. Magnetic ring; 15. Rotating shaft; 16. Fixing plate; 17. Spring; 18. Connecting plate two. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] The following describes an embodiment of this utility model based on its overall structure.

[0032] like Figures 1 to 6 As shown, this utility model provides a partitioned labeling culture dish for the confrontation culture of actinomycetes, including a second connecting plate 18. A rotating shaft 15 is fixedly provided on the upper end face of the second connecting plate 18. A magnetic suction ring 14 is provided on the outer side of the rotating shaft 15. A fixing plate 16 is fixedly provided on the outer side of the magnetic suction ring 14. A spring 17 is fixedly provided on the lower end face of the fixing plate 16, and the spring 17 is fixedly set with the second connecting plate 18. Through the magnetic suction ring 14, the fixing plate 16 and the second connecting plate 18 can be quickly attached or separated. When attached, the partitioned label can be stably fixed. When separated, it is convenient to inoculate the bacterial species into each partition. The operation does not require disassembling complex parts. Through the spring 17, the contact pressure between the fixing plate 16 and the plate can be buffered, which can not only avoid crushing the culture medium, but also automatically reset the fixing plate 16 when opening and closing, reducing manual adjustment steps.

[0033] In this embodiment, a magnetic column 8 is attached to the inner side of the magnetic ring 14, a connecting plate 7 is fixedly provided on the upper end surface of the magnetic column 8, and a partition plate 2 is fixedly provided on the outer side of the magnetic column 8. Through the partition plate 2, the culture dish has a partitioning function, which can separate different actinomycete strains and avoid mutual interference.

[0034] The partition plate 2 has a square groove 9 inside, and the magnetic column 8 is inside the square groove 9. The magnetic column 8 is embedded inside the partition plate 2 through the square groove 9, instead of the traditional external structure of "magnetic column 8 directly fixing the partition plate to the outside".

[0035] A partition plate 2 3 is fixedly provided on the outer side of the connecting plate 2 18. A slot 12 is provided inside the partition plate 2 3. A positioning block 4 is fixedly provided on the outer side of the partition plate 2 3. The partition plate 2 3 further enhances the partitioning function of the petri dish. The design of the slot 12 and the positioning block 4 provides convenience for the assembly and positioning of the petri dish.

[0036] A limiting plate 10 is fixedly provided on the lower end face of the partition plate 12, and a sealing plate 11 is fixedly provided on the lower end face of the limiting plate 10. A slot 2 13 is opened inside the partition plate 12, and the slot 2 13 is fitted with the slot 12. The limiting plate 10 and the sealing plate 11 enhance the sealing and stability of the petri dish.

[0037] A culture dish body 1 is fixedly provided on the outside of the sealing plate 11. A limiting groove 6 is opened inside the culture dish body 1, and the limiting groove 6 is fitted with the limiting plate 10. Through the limiting groove 6 and the limiting plate 10, the position of the partition plate 2 in the culture dish can be kept stable, thereby providing a stable environment for the culture of actinomycetes.

[0038] The petri dish body 1 has a positioning groove 5 inside, and the positioning groove 5 is fitted with the positioning block 4. Through the positioning groove 5 and the positioning block 4, the various parts of the petri dish can be tightly combined to form a complete whole, which improves the overall performance of the petri dish.

[0039] The working principle and procedure of using zone-labeled culture dishes for confrontation culture of actinomycetes:

[0040] Before using the petri dish, it needs to be cleaned and disinfected to ensure a sterile culture environment. At the same time, prepare the actinomycete strain and a suitable culture medium for its growth. Connect the connecting plate 2 18 to the petri dish body 1. The partition plate 2 3 on the outside of the connecting plate 2 18 corresponds to the positioning groove 5 inside the petri dish body 1. Insert the positioning block 4 into the positioning groove 5 to achieve the positioning and installation of the partition plate 2 3. Insert the magnetic column 8 into the square groove 9 inside the partition plate 1 2, so that the magnetic column 8 is aligned with the inner side of the magnetic ring 14. At this point, the separator plate 2 is attracted to the magnetic ring 14 on the connecting plate 2 18 via the magnetic column 8. During the connection process, the spring 17 acts as a buffer and stabilizer, ensuring a tight connection. The limiting plate 10 on the lower end face of the separator plate 2 is aligned with the limiting groove 6 inside the culture dish body 1 and inserted. At the same time, the sealing plate 11 is tightly fitted to the culture dish body 1, providing a sealing effect. Furthermore, the slot 2 13 inside the separator plate 2 fits into the slot 12 inside the separator plate 2 3, further enhancing the sealing effect. The internal partitioning stability of the petri dish is enhanced. After assembly, the interior of the petri dish is divided into different regions by partition plates 1-2 and 2-3. Different actinomycete species can be inoculated into each region according to experimental needs. This partitioning design effectively avoids mutual interference between different species and facilitates the observation and study of the confrontational relationships between different actinomycetes. The inoculated petri dishes are placed in a suitable culture environment, such as a constant temperature incubator, to provide suitable temperature, humidity, and other conditions for the growth of actinomycetes. During the culture process, due to the good sealing and partitioning structure of the petri dish, the culture conditions in each region are relatively independent and stable. The petri dishes are observed regularly, and the growth of actinomycetes in each region is recorded, including colony morphology, growth rate, and confrontational relationships. By observing the growth status of actinomycetes in different regions, the interaction and competition between actinomycetes can be studied in depth. After the experiment, the petri dishes are properly disposed of, such as being disinfected and discarded or washed and reused, to prevent the species from contaminating the environment.

[0041] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0042] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A zoned labeling culture dish for actinomycete plate confrontation culture, comprising a connecting plate two (18), characterized in that: The upper end face of the connecting plate 2 (18) is fixedly provided with a rotating shaft (15), and a magnetic ring (14) is provided on the outer side of the rotating shaft (15). A fixing plate (16) is fixedly provided on the outer side of the magnetic ring (14), and a spring (17) is fixedly provided on the lower end face of the fixing plate (16). The spring (17) is fixedly set with the connecting plate 2 (18).

2. The partitioned labeling culture dish for actinomycete confrontation culture according to claim 1, characterized in that: The magnetic ring (14) is fitted with a magnetic column (8) on its inner side. A connecting plate (7) is fixedly provided on the upper end face of the magnetic column (8). A partition plate (2) is fixedly provided on the outer side of the magnetic column (8).

3. The partitioned labeling culture dish for actinomycete confrontation culture according to claim 2, characterized in that: The partition plate 1 (2) has a square groove (9) inside, and the magnetic column (8) is inside the square groove (9).

4. The partitioned labeling culture dish for actinomycete confrontation culture according to claim 1, characterized in that: The outer side of the connecting plate 2 (18) is fixedly provided with a partition plate 2 (3), the partition plate 2 (3) is provided with a slot 1 (12), and the outer side of the partition plate 2 (3) is fixedly provided with a positioning block (4).

5. The partitioned labeling culture dish for actinomycete confrontation culture according to claim 2, characterized in that: The lower end face of the partition plate (2) is fixedly provided with a limiting plate (10), and the lower end face of the limiting plate (10) is fixedly provided with a sealing plate (11). The partition plate (2) is provided with a slot (13) inside, and the slot (13) fits into the slot (12).

6. The partitioned labeling culture dish for actinomycete confrontation culture according to claim 5, characterized in that: The outer side of the sealing plate (11) is fixedly provided with a petri dish body (1), and the petri dish body (1) has a limiting groove (6) inside, and the limiting groove (6) is fitted with the limiting plate (10).

7. The partitioned labeling culture dish for actinomycete confrontation culture according to claim 6, characterized in that: The petri dish body (1) has a positioning groove (5) inside, and the positioning groove (5) is fitted with the positioning block (4).

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