A device for preparing electrophoresis gel compatible with multiple protein detection

Abstract

The utility model belongs to the technical field of preparation device, especially kind of electrophoresis gel preparation device of compatible multiple protein detection, including preparation bucket body, the preparation bucket body is used for the preparation of electrophoresis gel, pivot, pivot and preparation bucket body inside stirring structure detachable connection, cleaning assembly, cleaning assembly is used for the preparation bucket body inside wall attachment carries out the cleaning, telescopic component, telescopic component and pivot are fixed, and be used for connecting cleaning assembly, this application drives the rod, first bevel gear, second bevel gear, threaded rod rotation in turn by driving part, threaded rod helps the threaded groove to drive the rod body along the shell movement, and the rod body moves in turn and makes the brush hair contact the inner wall of preparation bucket body, synchronously, pivot drives the brush hair rotation, and the brush hair cleans up the preparation bucket body inner wall attachment, reduces the material quantity change and the concentration change, guarantees the use effect of final preparation product.

Description

Technical Field

[0001] This invention belongs to the field of preparation device technology, and particularly relates to an electrophoretic gel preparation device that is compatible with the detection of multiple proteins. Background Technology

[0002] An electrophoresis gel preparation device compatible with multiple protein detection can adjust the gel formulation and operating parameters according to different experimental needs.

[0003] For example, Chinese patent CN218077812U discloses an electrophoretic gel preparation device that solves the problem that existing devices do not have a simple gel extraction structure, making it difficult to extract the gel manually and causing it to easily break.

[0004] When using this device, during the process of pouring agarose or polyacrylamide powder into the tank, some of the material will adhere to the inner wall of the tank. This will cause changes in the amount of material, which in turn will affect the concentration and thus the final product's performance. Utility Model Content

[0005] The purpose of this invention is to provide an electrophoresis gel preparation device compatible with the detection of multiple proteins, in order to solve the problems mentioned in the background art, including:

[0006] Prepare a barrel for preparing electrophoretic gels;

[0007] The rotating shaft is detachably connected to the stirring structure inside the preparation tank.

[0008] A cleaning component, used to clean the deposits adhering to the inner wall of the preparation tank;

[0009] A telescopic assembly, fixed to the rotating shaft and used to connect the cleaning assembly, allows the cleaning assembly to be in two states:

[0010] First state: The cleaning components are tightly attached to the inner wall of the preparation tank;

[0011] Second phase: Clean the components away from the inner wall of the preparation tank.

[0012] Preferably, the rotating shaft has a groove inside.

[0013] Preferably, the telescopic component includes:

[0014] A drive component, which is fixed to the rotating shaft, is used to provide power to the telescopic assembly;

[0015] A rotating rod, which is fixed to a driving component, passes through a rotating shaft and extends into the groove;

[0016] The first bevel gear is fixed to the rotating rod and is disposed in the groove.

[0017] The second bevel gear meshes with the first bevel gear, and the second bevel gear is disposed in the groove;

[0018] A threaded rod is fixed to a second bevel gear, and the threaded rod passes through a rotating shaft and is rotatably connected to the rotating shaft;

[0019] The housing is fixed to the outer wall of the rotating shaft, the threaded rod is disposed inside the housing, and the inner wall of the housing is polygonal.

[0020] Preferably, the cleaning component includes:

[0021] A rod body, wherein the rod body is disposed inside the housing and slidably connected to the inner wall of the housing, the rod body being polygonal and adapted to the housing;

[0022] A threaded groove is formed inside the rod body, and the threaded groove is threadedly connected to the threaded rod.

[0023] The plate body and the rod body are fixed together;

[0024] The brush bristles are fixed to the plate body and are used to clean the inner wall of the preparation barrel.

[0025] Preferably, the inner wall of the preparation barrel is fixedly connected with a protrusion, and the cleaning component can contact the protrusion when it is in the first state.

[0026] This application uses a drive component to sequentially rotate a rotating rod, a first bevel gear, a second bevel gear, and a threaded rod. The threaded rod, through its threaded groove, moves its body along the shell, while simultaneously moving the plate and brush bristles. This causes the brush bristles to contact the inner wall of the preparation tank. At the same time, the rotating shaft drives the brush bristles to rotate, and the brush bristles clean the deposits on the inner wall of the preparation tank, reducing material quantity and concentration variations and ensuring the final product's performance. Attached Figure Description

[0027] Figure 1 This is a structural diagram of the barrel body for the present invention;

[0028] Figure 2 This is a partial structural diagram of the present invention;

[0029] Figure 3 This is a cross-sectional view of the telescopic component of this utility model;

[0030] Figure 4 This is a cross-sectional view of the cleaning component of this utility model.

[0031] The markings in the diagram are as follows:

[0032] 100. Barrel body; 200. Rotating shaft; 210. Groove; 300. Telescopic assembly; 310. Drive component; 320. Rotating rod; 330. First bevel gear; 340. Second bevel gear; 350. Housing; 360. Threaded rod; 400. Cleaning assembly; 410. Rod body; 420. Threaded groove; 430. Plate body; 440. Brush bristles; 500. Raised strip. Detailed Implementation

[0033] The technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application are within the scope of protection of this application.

[0034] like Figure 1 As shown, this embodiment provides an electrophoresis gel preparation device compatible with the detection of multiple proteins, including: a preparation tank 100, a rotating shaft 200, a cleaning component 400, and a telescopic component 300.

[0035] The preparation tank 100 is used for preparing electrophoretic gels, and the preparation tank 100 can be the heating component disclosed in publication number CN218077812U. The preparation tank 100 is a conventional structure, such as a cylindrical shape with an internal cavity. The preparation tank 100 contains a conventional stirring structure for stirring the gel. This stirring structure is driven by a driving device located at the bottom of the cavity, which is also a conventional technology.

[0036] The rotating shaft 200 and the stirring structure inside the preparation tank 100 are detachably connected, so that when the stirring structure inside the preparation tank 100 rotates, it can drive the rotating shaft 200 to rotate synchronously.

[0037] The stirring structure inside the preparation tank 100 can be the turntable disclosed in announcement number CN218077812U. When it is not necessary to clean the inner wall of the preparation tank 100, the rotating shaft 200 can be disassembled.

[0038] The cleaning component 400 is used to clean the adhering substances on the inner wall of the preparation tank 100, thereby reducing the amount of material adhering to the inner wall of the tank when agarose or polyacrylamide powder is poured into the tank, reducing material quantity and concentration changes, and ensuring the final product's performance.

[0039] The telescopic component 300 is fixed to the rotating shaft 200 and is used to connect the cleaning component 400. The telescopic component 300 can change the position of the cleaning component 400, allowing the cleaning component 400 to be in two states: First state: The cleaning component 400 is in close contact with the inner wall of the preparation barrel 100. In this state, the cleaning component 400 can clean the deposits on the inner wall of the preparation barrel 100. Second state: The cleaning component 400 is away from the inner wall of the preparation barrel 100. In this state, the cleaning component 400 will not have frictional contact with the inner wall of the preparation barrel 100, thereby solving the problem of additional energy consumption caused by frictional contact.

[0040] In order for the telescopic component 300 to be able to change the position of the cleaning component 400, such as Figure 3 As shown, the rotating shaft 200 has a groove 210 inside, which is used to accommodate some components of the telescopic assembly 300, and the telescopic assembly 300 can complete vertical transmission within the groove 210. Figure 3 As shown, the telescopic assembly 300 includes: a drive component 310, a rotating rod 320, a first bevel gear 330, a second bevel gear 340, a housing 350, and a threaded rod 360. The drive component 310 is fixed to the rotating shaft 200. The drive component 310 drives the rotating rod 320 to rotate. The drive component 310 can be a motor or a manual knob. When the drive component 310 is a motor, it increases the automation of the device, allowing the telescopic assembly 300 to be driven by an external controller. When the drive component 310 is a manual knob, it can only drive the rotating rod 320 to rotate when the machine is stopped, but this is more energy-efficient. The choice should be made based on actual needs. The rotating rod 320 is a long, cylindrical shape, fixed to the drive component 310, and located on the rotating shaft 200, extending into the groove 210. The first bevel gear 330 is fixed to the rotating rod 320, so that the first bevel gear 330 can be driven to rotate when the rotating rod 320 rotates. The first bevel gear 330 is disposed in the groove 210. The second bevel gear 340 meshes with the first bevel gear 330, so that when the first bevel gear 330 is driven to rotate, it can drive the second bevel gear 340 to rotate. The second bevel gear 340 is disposed in the groove 210, and it can convert the rotation provided by the first bevel gear 330 into a vertical rotation. The threaded rod 360 is fixed to the second bevel gear 340, so that when the second bevel gear 340 rotates, it drives the threaded rod 360 to rotate. The threaded rod 360 is rotatably connected to the rotating shaft 200, and it can transmit the power provided by the second bevel gear 340, which is perpendicular to the direction of the rotating shaft 200 (e.g., ...). Figure 3The power source is located in the vertical direction. The housing 350 is polygonal in shape and is connected to the rotating shaft 200. The threaded rod 360 is located inside the housing. The outer walls of the housing 350 and the rotating shaft 200 are fixed together. The inner wall of the housing 350 is also polygonal. The housing 350 provides a certain amount of space for the movement of the cleaning assembly 400.

[0041] like Figure 4 As shown, the cleaning assembly 400 includes: a rod 410, a threaded groove 420, a plate 430, and bristles 440. The rod 410 is elongated and disposed within the housing 350, slidingly connected to the inner wall of the housing 350. The rod 410 has a polygonal shape and is adapted to fit the housing 350. The rod 410 can slide along the inner wall of the housing 350, and due to the polygonal shape, there is no relative rotation between the rod 410 and the housing 350. The rod 410 can move stably along the length of the housing 350. A threaded groove 420 is formed inside the rod 410, and the threaded groove 420 is threadedly connected to the threaded rod 360. When the threaded rod 360 rotates, the threaded groove 420 can drive the rod 410 to move along the length direction of the housing 350 with the power of the threaded rod 360. The plate 430 is fixed to the rod 410. When the rod 410 moves, the plate 430 will also move synchronously, and the plate 430 can install the bristles 440. The bristles 440 are fixed to the plate 430. The bristles 440 are used to clean the inner wall of the preparation barrel 100, and the movement of the plate 430 allows the bristles 440 to switch between a first state and a second state.

[0042] In further proposals, such as Figure 1 As shown, a protruding strip 500 is fixedly connected to the inner wall of the preparation barrel 100. The protruding strip 500 is elongated. When the cleaning component 400 is in the first state, it can contact the protruding strip 500. When the brush bristles 440 clean the agarose or polyacrylamide powder adhering to the inner wall of the barrel, the agarose or polyacrylamide powder may also adhere to the surface of the brush bristles 440. By setting the protruding strip 500, when the brush bristles 440 are in close contact with the inner wall of the preparation barrel 100, they will collide with the protruding strip 500 in stages. At this time, the brush bristles 440 will generate a certain vibration, thereby shaking off the agarose or polyacrylamide powder adhering to the surface of the brush bristles 440, reducing the amount of agarose or polyacrylamide powder adhering to the surface of the brush bristles 440, and at the same time reducing the change in material amount and concentration, ensuring the use effect of the final prepared product.

[0043] The embodiments of this application have been described above with reference to the accompanying drawings. Unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other. This application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.

Claims

1. An electrophoretic gel preparation device compatible with multiple protein detection methods, characterized in that, include: A preparation tank (100) is used for the preparation of electrophoretic gels, and a stirring structure is provided inside the tank. A rotating shaft (200) is detachably connected to the stirring structure inside the preparation tank (100); A cleaning component (400) is used to clean the deposits on the inner wall of the preparation barrel (100); A telescopic assembly (300) is fixed to a rotating shaft (200) and is used to connect a cleaning assembly (400). The telescopic assembly (300) enables the cleaning assembly (400) to have a first state and a second state. In the first state, the cleaning assembly (400) is in close contact with the inner wall of the preparation barrel (100). In the second state, the cleaning assembly (400) is away from the inner wall of the preparation barrel (100).

2. The electrophoretic gel preparation apparatus compatible with multiple protein detection according to claim 1, characterized in that, The rotating shaft (200) has a groove (210) inside.

3. The electrophoretic gel preparation apparatus compatible with multiple protein detection according to claim 2, characterized in that, The telescopic assembly (300) includes: A drive unit (310) is fixed to a rotating shaft (200) and the drive unit (310) is used to provide power to the telescopic assembly (300); A rotating rod (320) is fixed to a driving member (310). The rotating rod (320) passes through the rotating shaft (200) and extends into the groove (210). The first bevel gear (330) is fixed to the rotating rod (320), and the first bevel gear (330) is disposed in the groove (210); The second bevel gear (340) meshes with the first bevel gear (330), and the second bevel gear (340) is disposed in the groove (210); A threaded rod (360) is fixed to a second bevel gear (340), and the threaded rod (360) passes through a rotating shaft (200) and is rotatably connected to the rotating shaft (200); The outer wall of the housing (350) and the rotating shaft (200) are fixed together, the threaded rod (360) is disposed inside the housing (350), and the inner wall of the housing (350) is polygonal.

4. The electrophoretic gel preparation apparatus compatible with multiple protein detection according to claim 3, characterized in that, The cleaning component (400) includes: A rod (410) is disposed inside a housing (350) and slidably connected to the inner wall of the housing (350). The rod (410) is polygonal and adapted to the housing (350). A threaded groove (420) is formed inside the rod body (410), and the threaded groove (420) is threadedly connected to the threaded rod (360); Plate (430), wherein the plate (430) and the rod (410) are fixed together; Brush bristles (440) are fixed to the plate (430) and are used to clean the inner wall of the preparation barrel (100).

5. The electrophoretic gel preparation apparatus compatible with multiple protein detection according to claim 1, characterized in that, The inner wall of the preparation barrel (100) is fixedly connected with a protrusion (500), and the cleaning component (400) can contact the protrusion (500) when it is in the first state.

6. The electrophoretic gel preparation apparatus compatible with multiple protein detection according to claim 3, characterized in that, The drive unit (310) is a motor or a manual knob.

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