A polyacrylamide gel preparation device

Abstract

This utility model discloses a polyacrylamide gel preparation device, including a box body, multiple sets of glass plates, a pressing mechanism, and a rubber pad. The upper part of the box body is open, and the rubber pad is placed at the bottom of the box body. Each glass plate set includes two parallel glass pieces with a glass strip between their edges. When the two glass pieces are glued together, a gap for filling acrylamide solution is formed between them. Multiple sets of glass plates are placed side by side on the rubber pad inside the box body and are pressed tightly against each other. The pressing mechanism presses against the upper edge of the glass plate sets, thereby pressing the glass plate sets firmly onto the rubber pad. This polyacrylamide gel preparation device can significantly reduce gel leakage and can simultaneously prepare multiple polyacrylamide gels of basically the same size, thus effectively avoiding the impact of inconsistent sizes on protein analysis during electrophoresis, and has high practicality.

Description

Technical Field

[0001] This utility model relates to a polyacrylamide gel production device, and belongs to the field of polyacrylamide gel production technology. Background Technology

[0002] Polyacrylamide gel electrophoresis (PAG) is a classic method for protein analysis. Its core step is the preparation of the polyacrylamide gel. The existing method is as follows: 1. Use two glass plates, one of which has glass strips of a certain thickness attached to both sides; 2. Clamp the two glass plates in an electrophoresis tank and fix them to a base with a rubber pad. The bottom of the glass plates needs to be tightly sealed against the rubber pad (some methods also use a rubber strip between the two glass plates to seal the bottom gap. This strip needs to be removed after gelation, otherwise electrophoresis cannot be performed); 3. Pour the prepared acrylamide solution into the gap between the two glass plates and insert a comb (generally, a separating gel is poured first, followed by a water seal. After gelation, the water is removed, then a stacking gel is poured in, and the comb is inserted). After the acrylamide solution cross-links and forms a gel, the comb is removed.

[0003] However, a common problem in the second step is poor sealing of the bottom of the glass plate, causing the acrylamide solution to leak out slowly after being poured in, resulting in gel preparation failure. Additionally, this apparatus can only prepare a maximum of two gels at a time, limiting its capacity. Furthermore, it is difficult to control the amount of separating gel poured in to ensure that the two gels prepared simultaneously are of the same size. If the two gels undergoing electrophoresis at the same time differ significantly in size, it will adversely affect protein analysis. Utility Model Content

[0004] The technical problem to be solved by this utility model is to provide a glue-making device that can greatly reduce glue leakage and simultaneously prepare multiple polyacrylamide gels with each separating gel being roughly the same size.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0006] A polyacrylamide gel making apparatus includes a box body, multiple sets of glass plates, a pressing mechanism, and a rubber pad. The upper part of the box body is open, and the rubber pad is placed at the bottom of the box body. Each set of glass plates includes two parallel pieces of glass with a glass strip between their edges. When the two pieces of glass are glued together, a gap for filling acrylamide solution is formed between them. Multiple sets of glass plates are placed side by side on the rubber pad inside the box body and are pressed tightly against each other. The pressing mechanism presses the upper edge of the glass plate sets, thereby pressing the glass plate sets onto the rubber pad.

[0007] Preferably, the device further includes a pushing mechanism, which is installed on the side of the box and used to press the multiple glass plate groups against each other.

[0008] Preferably, the pushing mechanism includes a piston, a push rod, and a fixed seat. The fixed seat is fixedly installed on the side of the box body. One end of the push rod is connected to the piston and placed inside the box body, and the other end is a screw with a threaded section. A threaded hole that engages with the screw is provided in the middle of the fixed seat. The screw passes through the side wall of the box body and is threadedly connected to the fixed seat and can rotate freely left and right, thereby driving the piston to move freely left and right inside the box body. The extension and retraction direction of the screw is perpendicular to the glass surface of the glass plate assembly. The piston is movably connected to the end of the push rod, and the push rod rotates freely relative to the piston.

[0009] Preferably, the pressing mechanism is a pressure strip, which presses against the upper edges of the glass on both sides.

[0010] Preferably, the outer side of the box is provided with scale lines that can display the liquid level inside the box.

[0011] Preferably, the box body is provided with a pressing device on both the front and rear sides. The pressing device includes an upper pressing plate, a lower pressing plate and two screws. The upper pressing plate presses against the pressing strip, the lower pressing plate is placed on the bottom side of the box body, and the two screws are respectively vertically opposite to each other and pass through the upper pressing plate and the lower pressing plate. The lower end of the screw has a fixed end and the upper end is threaded with a top knob.

[0012] Compared with the prior art, the advantages of this utility model are: the polyacrylamide gel making device adopts a piston-type pushing mechanism to press multiple sets of glass plates for simultaneous gel making, which can significantly reduce gel leakage, and can simultaneously prepare multiple polyacrylamide gels of basically the same size, thereby effectively avoiding the impact on protein analysis caused by inconsistent sizes during electrophoresis, and has high practicality. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0014] Figure 1 This is a top view of the present invention without the pressing mechanism and press device installed on one side;

[0015] Figure 2 This is a partial sectional view of the side of this utility model. Detailed Implementation

[0016] The technical solutions in the embodiments of this utility model will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0017] like Figure 1 , Figure 2 The apparatus shown includes a polyacrylamide gel making device, comprising a box body 1, multiple sets of glass plates, a pressing mechanism, and a rubber pad. The upper part of the box body 1 is open, and the rubber pad is placed at the bottom of the box body 1. The glass plate sets include two parallel glass pieces 2, with a glass strip 3 between the edges of the two glass pieces 2. When the two glass pieces 2 are glued together, a gap is formed between the glass pieces for filling acrylamide solution. The multiple sets of glass plates are placed side by side on the rubber pad inside the box body 1 and are pressed against each other. The pressing mechanism presses the upper edge of the glass plate sets and then presses the glass plate sets onto the rubber pad.

[0018] In this embodiment, to facilitate the clamping of the glass plate assemblies, the device further includes a pushing mechanism. The pushing mechanism is installed on the side of the housing 1 and is used to clamp multiple glass plate assemblies together. Further, to facilitate stable pushing and clamping of the glass 2, the pushing mechanism includes a piston 4, a push rod 5, and a fixed seat 6. The fixed seat 6 is fixedly installed on the side of the housing 1. One end of the push rod 5 is connected to the piston 4 and placed inside the housing 1, while the other end is a screw with a threaded section. A threaded hole is provided in the middle of the fixed seat to engage with the screw thread. The screw passes through the side wall of the housing and is threadedly connected to the fixed seat 6, allowing it to rotate freely left and right. This drives the piston 4 to move freely left and right within the housing 1. The extension and retraction direction of the screw is perpendicular to the glass surface of the glass plate assembly. The piston 4 is movably connected to the end of the push rod 5, allowing the push rod to rotate freely relative to the piston. The other end of the screw extends out of the fixed seat 6 and is connected to an end knob 7. Subsequently, in practical applications, by turning the screw through the end knob 7, the screw, i.e. the push rod, can rotate freely relative to the piston and move linearly. When it moves towards the glass side, it can press multiple sets of glass plates together, thereby pressing and fixing the glass plates together. The glass plates are completely bonded together without gaps, thus ensuring the stability of the gelation process.

[0019] In this embodiment, the pressing mechanism is a pressing strip 8. The pressing strip 8 presses against the upper two edges of the glass 2, thereby pressing the glass 2 against the rubber pad, keeping the bottom of the glass plate in close contact with the rubber pad, maintaining the seal between the two, and preventing the bottom from leaking glue. To further press the pressing strip, pressing devices are provided on both the front and rear sides of the box body. The pressing device includes an upper pressing plate 9, a lower pressing plate 10, and two screws 11. The upper pressing plate 9 presses against the pressing strip 8, and the lower pressing plate 10 is placed on the bottom side of the box body 1. The two screws 11 are respectively vertically opposite each other and pass through the upper pressing plate 9 and the lower pressing plate 10. The lower end of the screw 11 has a fixed end, and the upper end is threaded with a top knob 12. By simultaneously rotating the top knob 12 at the upper end of the two screws, the knob rotates on the screw and moves downward to press the upper pressing plate 9, thereby pressing the upper pressing plate 9 and the lower pressing plate 10 together, and pressing the pressing strip 8 against the upper end of the glass plate.

[0020] In this embodiment, the outer side of the box 1 is provided with a scale line that can display the liquid level in the box, so as to facilitate the filling of glue and water according to the scale line indication.

[0021] In the actual gel preparation, the main steps are as follows: First, place the glass plate group (several groups can be placed if the box can accommodate them) into the box 1, rotate the top rod 5 to drive the piston 4 to move linearly and then hold the glass plate 2 in place, then put down the pressure strip 8 and press the pressure strip 8 with the pressing device to press down on both sides of the glass plate 2, so that the bottom of the glass plate is in close contact with the gel pad; Second, pour the prepared acrylamide solution into the gap between the two glass plates in each group, and control the volume of the added acrylamide solution by the position relationship between the liquid level and the scale line, so that the size of each separating gel is basically the same; Third, add an appropriate amount of water into the gap between the two glass plates in each group, and at the same time pour water into the box, so that the water level in the box is close to the height of the separating gel and water in the gap between the glass plates. In this way, even if there is a small gap between the bottom of the glass plate and the gel pad, the water inside the box will ensure that the separating gel does not leak out; fourth, after the separating gel gels, remove the water on top, pour in the building block gel, insert the comb, and after the building block gel gels, the glass plate and polyacrylamide gel can be placed in the electrophoresis tank for use, thus completing the gel preparation process.

[0022] It should be emphasized that the above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model in any way. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model shall still fall within the scope of the technical solution of the present utility model.

Claims

1. A polyacrylamide gel preparation apparatus, characterized in that: The device includes a box body, multiple sets of glass plates, a pressing mechanism, and a rubber pad. The box body has an opening at the top, and the rubber pad is placed at the bottom of the box body. Each set of glass plates includes two parallel pieces of glass with a glass strip between their edges. When the two pieces of glass are glued together, a gap is formed between them for filling with acrylamide solution. Multiple sets of glass plates are placed side by side on the rubber pad inside the box body and are pressed tightly against each other. The pressing mechanism presses the upper edge of the glass plate sets, thereby pressing the glass plate sets firmly onto the rubber pad.

2. The polyacrylamide gel preparation apparatus according to claim 1, characterized in that: The device also includes a pushing mechanism, which is installed on the side of the box and used to press the multiple glass plate groups against each other.

3. The polyacrylamide gel preparation apparatus according to claim 2, characterized in that: The pushing mechanism includes a piston, a push rod, and a fixed seat. The fixed seat is fixedly installed on the side of the box body. One end of the push rod is connected to the piston and placed inside the box body, and the other end is a screw with a threaded section. A threaded hole that engages with the screw is provided in the middle of the fixed seat. The screw passes through the side wall of the box body and is threadedly connected to the fixed seat and can rotate freely left and right, thereby driving the piston to move freely left and right inside the box body. The extension and retraction direction of the screw is perpendicular to the glass surface of the glass plate assembly. The piston is movably connected to the end of the push rod, and the push rod rotates freely relative to the piston.

4. The polyacrylamide gel preparation apparatus according to claim 1, characterized in that: The pressing mechanism is a pressure strip, which presses against the upper edges of the glass on both sides.

5. The polyacrylamide gel preparation apparatus according to claim 4, characterized in that: The box body is equipped with a clamping device on both the front and rear sides. The clamping device includes an upper pressure plate, a lower pressure plate and two screws. The upper pressure plate is pressed against the pressure strip, and the lower pressure plate is placed on the bottom side of the box body. The two screws are respectively vertically opposite to each other and pass through the upper pressure plate and the lower pressure plate. The lower end of the screw has a fixed end and the upper end is threaded with a top knob.

6. The apparatus for preparing polyacrylamide gel according to claim 1, characterized in that: The outer side of the box is equipped with scale lines that can display the liquid level inside the box.

Images