Beauveria solid culture inoculation device
By designing a Beauveria bassiana solid culture inoculation device that includes a rotating disc driven by a stirring motor and stirring blades, the problem of uneven mixing of solid culture medium was solved, and a more uniform inoculation effect was achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- GUIZHOU TOBACCO CORP QIANXINAN CORP
- Filing Date
- 2025-06-16
- Publication Date
- 2026-06-02
AI Technical Summary
Existing solid culture inoculation devices do not mix solid culture media evenly enough, resulting in incomplete inoculation.
A solid culture inoculation device for Beauveria bassiana was designed, comprising a box, a mixing cylinder, a turntable, a stirring shaft, and stirring blades. The combined movement of the stirring shaft and the turntable is driven by a stirring motor to achieve thorough stirring of the solid culture medium, and the inoculation solution is evenly distributed through atomizing nozzles.
It achieves uniform stirring of solid culture medium and uniform mixing of inoculation solution, improving the inoculation effect. The device has a simple structure and can be reused.
Smart Images
Figure CN224313499U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the technical field of Beauveria bassiana culture equipment, specifically relating to a Beauveria bassiana solid culture inoculation device. Background Technology
[0002] Beauveria bassiana is an entomopathogenic fungus belonging to the ascomycetes. The main species include Beauveria bassiana and Beauveria bryonis. It usually reproduces asexually by producing conidia. The hyphae have septa and segments. Beauveria bassiana can invade the bodies of more than 200 kinds of insects and mites in 6 orders and 15 families and multiply in large quantities. At the same time, it produces beauveria bassiana (a non-ribosomal polypeptide toxin), oosporin (a benzoquinone toxin), and calcium oxalate crystals. These substances can cause insect poisoning, disrupt metabolism, and lead to death.
[0003] Beauveria bassiana can be cultivated using both liquid and solid-state fermentation methods. During solid-state fermentation, the solid culture medium needs to be stirred to prevent clumping and to ensure adequate inoculation.
[0004] Existing solid culture inoculation devices do not mix solid culture media evenly enough. Utility Model Content
[0005] The technical problem to be solved by this utility model is to provide a Beauveria bassiana solid culture inoculation device to address the shortcomings of the prior art. The device has a simple structure, can fully stir the solid culture medium and inoculation solution, is convenient to discharge, can be reused, and can be widely applied.
[0006] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is: a Beauveria bassiana solid culture inoculation device, characterized in that it includes a box body, a seed mixing cylinder is fixedly installed inside the box body, a turntable is installed on the top of the box body via a bearing, a stirring shaft is vertically eccentrically inserted on the turntable via a bearing, each bearing is provided with a bearing sealing cover, a plurality of stirring blades are equidistantly arranged on one end of the stirring shaft extending into the seed mixing cylinder, a stirring motor for driving the stirring shaft is fixedly installed on the turntable, a rotation motor for driving the turntable is fixedly installed on the top of the box body, an inoculation tank is fixedly installed on the turntable, a connecting pipe is fixedly connected to the side end of the inoculation tank, an annular pipe is fixedly installed at the bottom end of the turntable, the annular pipe communicates with the connecting pipe, and a feed inlet for adding solid culture medium into the seed mixing cylinder is provided on the side wall of the box body, and a sealing plug is provided on the feed inlet.
[0007] The solid culture medium is made by soaking wheat in water at a weight ratio of 1:1.5 and a soaking time of 4 hours. After soaking, it is placed in a heat-resistant plastic bag and sterilized at 100°C for 6-8 hours. After sterilization, it is cooled and ready for use.
[0008] The stirring motor drives the stirring shaft to rotate, and the stirring blades on the stirring shaft rotate to stir the solid culture medium, so that the inoculum and the solid culture medium are mixed evenly.
[0009] The rotating motor drives the turntable to rotate, and the stirring shaft on the turntable rotates around the rotating shaft. This causes the stirring range of the stirring blades to change with the rotation of the turntable, allowing the stirring blades to get closer to the inner wall of the seed mixing cylinder, resulting in more thorough and even mixing.
[0010] Preferably, a first pulley is fixedly installed on the top end of the stirring shaft and the end of the output shaft of the stirring motor, and the two first pulleys are connected by a first belt drive. A rotating shaft is fixedly installed at the center of the turntable, and a second pulley is fixedly installed on the top end of the rotating shaft and the end of the output shaft of the rotating motor. The two second pulleys are connected by a second belt drive, and the installation position of the first pulley is lower than the installation position of the second pulley.
[0011] Preferably, the inoculation tank is equipped with a delivery pump connected to a connecting pipe, and multiple atomizing nozzles are evenly fixedly installed at the bottom end of the annular pipe. The delivery pump transports the inoculation solution from the inoculation tank into the annular pipe, and finally the atomizing nozzles spray it into the inoculation tank, so that the inoculation solution is evenly distributed.
[0012] Preferably, ventilation interfaces are embedded in the side walls of the box located on opposite sides of the seed mixing cylinder. Each ventilation interface is equipped with a solenoid valve; one interface is an inlet, and the other is an outlet. The inlet can be connected to high-temperature steam for high-temperature sterilization of the box, or to sterile air for air circulation within the seed mixing cylinder.
[0013] Preferably, a discharge cylinder is embedded at the bottom of the seed mixing cylinder, and discharge pipes are fixedly inserted on both sides of the discharge cylinder. A receiving tray is provided on the bottom of the box body directly below the outlet of the discharge pipe. An electric push rod is fixedly installed at the bottom of the discharge cylinder. A sealing plate is fixedly installed at the top of the output shaft of the electric push rod. A sealing ring is provided between the outer periphery of the sealing plate and the inner wall of the discharge cylinder. The top surface of the sealing plate is convex.
[0014] The electric pusher moves the sealing disc up and down within the discharge cylinder. When the electric pusher extends to its maximum stroke, the top edge of the sealing disc is flush with the bottom of the mixing cylinder, disconnecting the discharge cylinder from the mixing cylinder. After inoculation, the output shaft of the electric pusher retracts to its minimum stroke, and the top surface of the sealing disc is higher than the interface between the discharge pipe and the discharge cylinder. The solid culture medium and inoculum in the mixing cylinder can then flow from the mixing cylinder through the discharge cylinder into the discharge pipe, and from the discharge pipe into the receiving tray for further processing.
[0015] Preferably, the bottom of the box is provided with sealing doors on both sides to keep the inside of the box sealed during seed mixing and inoculation.
[0016] This utility model has the following advantages compared with the prior art:
[0017] This invention features a turntable mounted on the top of the housing. A rotating motor drives the turntable to rotate. An eccentrically inserted stirring shaft is mounted on the turntable, with stirring blades evenly spaced on the stirring shaft. A stirring motor is fixedly mounted on the turntable, driving the stirring shaft to rotate. This causes the stirring shaft to revolve around the rotating shaft while rotating on its own axis, expanding the stirring range and allowing the stirring blades to move closer to the inner wall of the mixing cylinder, resulting in a more thorough and uniform stirring effect.
[0018] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the structure of this utility model.
[0020] Explanation of reference numerals in the attached figures:
[0021] Detailed Implementation
[0022] To make the above-mentioned objectives, features, and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a full understanding of this utility model. However, this utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed below.
[0023] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly attached to the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.
[0024] like Figure 1As shown, this utility model provides a Beauveria bassiana solid culture inoculation device, including a box body 1, a seed mixing cylinder 2 fixedly installed inside the box body 1, a turntable 3 mounted on the top of the box body 1 via bearings, a stirring shaft 4 vertically and eccentrically inserted on the turntable 3 via bearings, each bearing being provided with a bearing sealing cover, a plurality of stirring blades 5 evenly arranged on one end of the stirring shaft 4 extending into the seed mixing cylinder 2, a stirring motor 6 for driving the stirring shaft 4 fixedly installed on the turntable 3, a rotation motor 7 for driving the turntable 3 fixedly installed on the top of the box body 1, an inoculation tank 8 fixedly installed on the turntable 3, a connecting pipe fixedly connected to the side end of the inoculation tank 8, an annular pipe 9 fixedly installed at the bottom end of the turntable 3, the annular pipe 9 communicating with the connecting pipe, and a feed inlet 13 for adding solid culture medium into the seed mixing cylinder 2 provided on the side wall of the box body 1, the feed inlet 13 being provided with a sealing plug.
[0025] The solid culture medium is made by soaking wheat in water at a weight ratio of 1:1.5 and a soaking time of 4 hours. After soaking, it is placed in a heat-resistant plastic bag and sterilized at 100°C for 6-8 hours. After sterilization, it is cooled and ready for use.
[0026] The stirring motor 6 drives the stirring shaft 4 to rotate, and the stirring blades 5 on the stirring shaft 4 rotate to stir the solid culture medium, so that the inoculum and the solid culture medium are mixed evenly.
[0027] The rotating motor 7 drives the turntable 3 to rotate, and the stirring shaft 4 on the turntable 3 rotates around the rotating shaft 11, so that the stirring range of the stirring blade 5 changes with the rotation of the turntable 3. The stirring blade 5 can be closer to the inner wall of the seed mixing cylinder 2, and the stirring is more thorough and uniform.
[0028] In this embodiment, a first pulley is fixedly installed on the top end of the stirring shaft 4 and the end of the output shaft of the stirring motor 6. The two first pulleys are connected by a first belt 10. A rotating shaft 11 is fixedly installed at the center of the turntable 3. A second pulley is fixedly installed on the top end of the rotating shaft 11 and the end of the output shaft of the rotating motor 7. The two second pulleys are connected by a second belt 12. The installation position of the first pulley is lower than the installation position of the second pulley.
[0029] In this embodiment, the inoculation tank 8 is equipped with a delivery pump connected to the connecting pipe, and multiple atomizing nozzles are uniformly fixedly installed at the bottom end of the annular pipe 9. The delivery pump delivers the inoculation solution in the inoculation tank 8 to the annular pipe 9, and finally sprays it into the inoculation tank 8 through the atomizing nozzles, so that the inoculation solution is evenly distributed.
[0030] In this embodiment, ventilation ports 14 are embedded in the side walls of the box 1 located on opposite sides of the seed mixing cylinder 2. Each ventilation port 14 is equipped with a solenoid valve. One ventilation port 14 is an inlet, and the other is an outlet. The inlet can be connected to high-temperature steam for high-temperature sterilization of the box 1, or to sterile air to allow air circulation within the seed mixing cylinder 2.
[0031] In this embodiment, a discharge cylinder 15 is embedded at the bottom of the seed mixing cylinder 2, and discharge pipes 16 are fixedly inserted on both sides of the discharge cylinder 15. A receiving plate 17 is provided on the bottom of the box body 1 directly below the outlet of the discharge pipe 16. An electric push rod 18 is fixedly installed at the bottom of the discharge cylinder 15. A sealing plate 19 is fixedly installed at the top of the output shaft of the electric push rod 18. A sealing ring is provided between the outer periphery of the sealing plate 19 and the inner wall of the discharge cylinder 15. The top surface of the sealing plate 19 is convex.
[0032] The electric push rod 18 can move the sealing disc 19 up and down inside the discharge cylinder 15. When the electric push rod 18 extends to its maximum stroke, the top edge of the sealing disc 19 is flush with the bottom end of the seed mixing cylinder 2, disconnecting the connection between the discharge cylinder 15 and the seed mixing cylinder 2. After the seed mixing and inoculation are completed, the output shaft of the electric push rod 18 retracts to its minimum stroke, and the top surface of the sealing disc 19 is higher than the interface between the discharge pipe 16 and the discharge cylinder 15. The solid culture medium and inoculum in the seed mixing cylinder 2 can enter the discharge pipe 16 from the seed mixing cylinder 2 through the discharge cylinder 15, and then be transferred from the discharge pipe 16 to the receiving tray 17 for subsequent processing.
[0033] In this embodiment, the bottom two sides of the box 1 are provided with sealing doors to keep the inside of the box 1 sealed during seed mixing and inoculation.
[0034] When in use, fill the inoculation tank 8 with inoculation solution and add a certain amount of solid culture medium into the seed mixing cylinder 2 through the feed inlet 13.
[0035] Start the stirring motor 6 to drive the stirring shaft 4 to rotate. The stirring blades 5 on the stirring shaft 4 rotate to stir the solid culture medium, so that the inoculum and solid culture medium are mixed evenly.
[0036] At the same time, the rotating motor 7 is started, which drives the turntable 3 to rotate. The stirring shaft 4 on the turntable 3 rotates around the rotating shaft 11, so that the stirring range of the stirring blade 5 changes with the rotation of the turntable 3. The stirring blade 5 can be closer to the inner wall of the seed mixing cylinder 2, and the stirring is more thorough and uniform.
[0037] At the same time, the delivery pump inside the inoculation tank 8 is started, and the inoculation solution is delivered to the annular pipe 9 through the delivery pump. Finally, it is sprayed into the inoculation tank 8 by the atomizing nozzle to make the inoculation solution evenly distributed.
[0038] After the seed treatment and inoculation are completed, turn off the mixing motor 6, the rotating motor 7, and the delivery pump.
[0039] When the electric push rod 18 is activated, the output shaft of the electric push rod 18 retracts to its minimum stroke. The top surface of the sealing plate 19 is higher than the interface between the discharge pipe 16 and the discharge cylinder 15. The solid culture medium and inoculum in the mixing cylinder 2 can enter the discharge pipe 16 from the mixing cylinder 2 through the discharge cylinder 15, and then be transferred from the discharge pipe 16 to the receiving tray 17 for subsequent processing.
[0040] The above description is merely a preferred embodiment of this utility model and does not constitute any limitation on this utility model. Any simple modifications, alterations, and equivalent changes made to the above embodiments based on the technical essence of this utility model shall still fall within the protection scope of this utility model.
Claims
1. A Beauveria bassiana solid culture inoculation device, characterized in that, The device includes a housing (1), a seed mixing cylinder (2) is fixedly installed inside the housing (1), a turntable (3) is installed on the top of the housing (1) via a bearing, a stirring shaft (4) is vertically eccentrically inserted on the turntable (3) via a bearing, a plurality of stirring blades (5) are equidistantly arranged on one end of the stirring shaft (4) extending into the seed mixing cylinder (2), a stirring motor (6) for driving the stirring shaft (4) to rotate is fixedly installed on the turntable (3), a rotating motor (7) for driving the turntable (3) to rotate is fixedly installed on the top of the housing (1), an inoculation tank (8) is fixedly installed on the turntable (3), a connecting pipe is fixedly connected to the side end of the inoculation tank (8), an annular pipe (9) is fixedly installed at the bottom end of the turntable (3), the annular pipe (9) is connected to the connecting pipe, and an inlet (13) for adding solid culture medium into the seed mixing cylinder (2) is provided on the side wall of the housing (1).
2. The Beauveria bassiana solid culture inoculation device according to claim 1, characterized in that, A first pulley is fixedly installed on the top of the stirring shaft (4) and the end of the output shaft of the stirring motor (6). The two first pulleys are connected by a first belt (10). A rotating shaft (11) is fixedly installed at the center of the turntable (3). A second pulley is fixedly installed on the top of the rotating shaft (11) and the end of the output shaft of the rotating motor (7). The two second pulleys are connected by a second belt (12). The installation position of the first pulley is lower than the installation position of the second pulley.
3. The Beauveria bassiana solid culture inoculation device according to claim 1, characterized in that, The inoculation tank (8) is equipped with a delivery pump connected to the connecting pipe, and multiple atomizing nozzles are uniformly fixedly installed at the bottom end of the annular pipe (9).
4. The Beauveria bassiana solid culture inoculation device according to claim 1, characterized in that, Ventilation ports (14) are installed on the side walls of the box (1) located on opposite sides of the seed mixing cylinder (2), and solenoid valves are provided on the ventilation ports (14).
5. The Beauveria bassiana solid culture inoculation device according to claim 1, characterized in that, The bottom end of the seed mixing cylinder (2) is fitted with a discharge cylinder (15), and discharge pipes (16) are fixedly inserted on both sides of the discharge cylinder (15). A receiving tray (17) is provided on the bottom end of the box body (1) directly below the outlet of the discharge pipe (16). An electric push rod (18) is fixedly installed at the bottom end of the discharge cylinder (15). A sealing disc (19) is fixedly installed at the top end of the output shaft of the electric push rod (18). A sealing ring is provided between the outer periphery of the sealing disc (19) and the inner wall of the discharge cylinder (15). The top surface of the sealing disc (19) is convex.
6. The Beauveria bassiana solid culture inoculation device according to claim 1, characterized in that, The box (1) has sealed doors on both sides of its bottom end.