A rapid detection device for biomolecular markers

Abstract

This utility model discloses a rapid detection device for biomolecular markers, comprising a main body with several symmetrically arranged insertion slots on its upper and lower surfaces. A through hole is formed within the main body, connecting the upper and lower insertion slots. An installation groove is formed on the inner wall of each insertion slot, and an elastic compression plate is fixedly connected within the installation groove. An outer tube is inserted into the upper insertion slot, and an outlet tube is inserted into the lower insertion slot. An inner tube is disposed within the outer tube, and an outwardly expanding cone is fixedly connected to the upper end of the outer tube. A limiting flange is fixedly connected within the through hole. By inserting the outer tube and outlet tube into the insertion slots from their respective upper and lower ends, the elastic compression plate is compressed and deformed upon insertion. The elastic compression plate, possessing a certain elasticity, conforms to and compresses the outer walls of the outer tube and outlet tube, generating friction. This friction is used to relatively fix the outer tube and outlet tube within the insertion slots, making disassembly and installation more convenient.

Description

Technical Field

[0001] This utility model relates to a rapid detection device, and more particularly to a rapid detection device for biomolecular markers. Background Technology

[0002] Currently, the detection of biomolecular markers is mainly based on enzyme chain immunoassay (ELISA). For example, biomolecular markers used for disease diagnosis are analyzed and quantified using this method. The principle of ELISA is basically to fix antibodies or antigens on the surface of the wells of a 96-well plate, bind to the corresponding antigens or antibodies in the sample, then introduce secondary antibodies, add substrates, and react to produce chemiluminescence or fluorescence. Finally, an enzyme-linked immunosorbent assay (ELISA) reader is usually used to detect the chemiluminescence or fluorescence signal produced by the 96-well plate.

[0003] For example, Chinese Patent Publication No. CN202221388957.4 discloses a rapid detection device for biomolecular markers, comprising a main body with several through holes; an inlet tube inserted into the upper part of the through holes via a connecting structure; an outlet tube adapted to the inlet tube and inserted into the bottom of the through holes via a connecting structure; a filter membrane disposed between the inlet tube and the outlet tube via an installation structure; and a control system. The inlet tube includes an outer tube, an inner tube inserted into the outer tube via a connecting rod, and two optical fibers disposed inside the inner canister. By using an inlet tube and an outlet tube respectively positioned at the top and bottom, high throughput is achieved, allowing for the simultaneous detection of multiple biomolecular markers. The device is simple to operate, highly automated, and has high detection efficiency.

[0004] The aforementioned rapid detection device for biomolecular markers has some problems in use. First, the connection structure of the threaded mounting base and the mounting groove, which installs the outlet tube and the inlet tube at the bottom of the through hole, requires frequent twisting of the tube body during installation and disassembly, which is extremely inconvenient. Second, a channel needs to be formed between the inner tube and the aforementioned outer tube for the addition of detection reagents, but the gap at the upper end of the channel is small, which requires extreme care during the addition process. Utility Model Content

[0005] The purpose of this invention is to provide a rapid detection device for biomolecular markers to solve the existing problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a rapid detection device for biomolecular markers, comprising a main body, wherein a plurality of insertion slots are symmetrically opened on the upper and lower surfaces of the main body, a through hole is opened in the main body, the through hole connects the upper and lower insertion slots, an installation groove is opened on the inner wall of the insertion slot, an elastic extrusion piece is fixedly connected in the installation groove, an outer tube is inserted into the upper insertion slot, an outlet tube is inserted into the lower insertion slot, an inner tube is provided inside the outer tube, and an outwardly expanding cone head is fixedly connected to the upper end of the outer tube.

[0007] Preferably, a limiting flange is fixedly connected inside the through hole, a mesh plate is placed inside the through hole and above the limiting flange, and a filter membrane is placed inside the through hole and above the mesh plate.

[0008] Preferably, a connecting piece is fixedly connected to the inner wall of the outer tube, and the other side of the connecting piece is fixedly connected to the inner tube.

[0009] Preferably, a pressing flange is fixedly connected to the bottom of the outer tube, and the pressing flange cooperates with the limiting flange.

[0010] Compared with the prior art, the beneficial effects of this utility model are:

[0011] 1. By inserting the outer tube and the outlet tube into the insertion slot from the top and bottom respectively, the elastic compression plate will deform after insertion. The elastic compression plate itself has a certain elasticity and will conform to the outer wall of the outer tube and the outlet tube to generate a certain friction. The friction is used to fix the outer tube and the outlet tube relatively inside the insertion slot, which is more convenient to disassemble and install than the existing device.

[0012] 2. The diameter of the outer tube is increased by the outer expansion cone fixed to the upper end of the outer tube, while the upper end of the inner tube is higher than the upper end of the outer expansion cone, which makes it easier for the operator to inject the test liquid from the gap between the outer tube and the inner tube into the through hole through the outer expansion cone.

[0013] 3. The filter membrane and screen are placed directly inside the through hole. By using the extrusion flange and the limiting flange to work together, the filter membrane and screen can be squeezed and fixed inside the through hole, preventing the filter membrane and screen from shaking or moving during the testing process. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0015] Figure 2 This is a cross-sectional view of the present invention;

[0016] Figure 3 For the present utility model Figure 2 Enlarged view of point A in the middle;

[0017] Figure 4This is a schematic diagram of the outer tube structure of this utility model.

[0018] In the diagram: 1. Main body; 101. Insertion groove; 102. Through hole; 103. Limiting flange; 104. Mounting groove; 105. Elastic extrusion piece; 2. Outer tube; 201. Inner tube; 202. Connecting piece; 203. Extrusion flange; 204. Outwardly expanding cone; 3. Outlet tube; 4. Filter membrane; 5. Mesh plate. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figure 1-4 This utility model provides a technical solution: a rapid detection device for biomolecular markers, including a main body 1. The main body 1 has a plurality of insertion slots 101 symmetrically opened on the upper and lower surfaces. The main body 1 has a through hole 102, which connects the upper and lower insertion slots 101. The inner wall of the insertion slot 101 has an installation slot 104. An elastic extrusion piece 105 is fixedly connected in the installation slot 104. An outer tube 2 is inserted into the upper insertion slot 101, and an outlet tube 3 is inserted into the lower insertion slot 101. An inner tube 201 is provided inside the outer tube 2, and an outwardly expanding cone 204 is fixedly connected to the upper end of the outer tube 2.

[0021] In this embodiment, the outer tube 2 and the outlet tube 3 are inserted into the insertion slot 101 from the upper and lower ends, respectively. After insertion, the outer walls of the outer tube 2 and the outlet tube 3 compress the elastic compression plate 105, causing the elastic compression plate 105 to deform. The elastic compression plate 105 is made of elastic material. The deformation of the elastic compression plate 105 compresses the outer tube 2 and the outlet tube 3, thereby using friction to fix the outer tube 2 and the outlet tube 3 relatively inside the insertion slot 101. Insertion and removal require a certain force to overcome friction. Compared with existing devices, disassembly and removal are more efficient. Installation is more convenient, but the stability is somewhat reduced. However, no large shaking or impact is required during the detection process, which can meet the normal use of the device. The inner tube 201 is located inside the outer tube 2. An optical fiber for detection is inserted into the inner tube 201 to determine whether to collect chemiluminescence or fluorescence signals. The diameter of the outer tube 204, which is fixed to the upper end of the outer tube 2, is enlarged. At the same time, the upper end of the inner tube 201 is higher than the upper end of the outer tube 204, which makes it easier for the operator to add the detection liquid from the gap between the outer tube 2 and the inner tube 201 into the through hole 102 through the outer tube 204.

[0022] In order to fix the filter membrane 4 and the mesh plate 5, the device adopts the following technical solution: a limiting flange 103 is fixedly connected inside the through hole 102, a mesh plate 5 is placed inside the through hole 102 and above the limiting flange 103, a filter membrane 4 is placed inside the through hole 102 and above the mesh plate 5, a connecting piece 202 is fixedly connected to the inner wall of the outer tube 2, the other side of the connecting piece 202 is fixedly connected to the inner tube 201, and a pressing flange 203 is fixedly connected to the bottom of the outer tube 2, the pressing flange 203 and the limiting flange 103 cooperate with each other.

[0023] After the outer tube 2 is inserted into the insertion slot 101, the extrusion flange 203 is inserted into the through hole 102, and the filter membrane 4 and the mesh plate 5 are placed directly inside the through hole 102. By using the cooperation of the extrusion flange 203 and the limiting flange 103, the filter membrane 4 and the mesh plate 5 can be squeezed and fixed inside the through hole 102, so as to avoid the filter membrane 4 and the mesh plate 5 shaking or moving during the detection process. The inner tube 201 is fixed inside the outer tube 2 by the connecting piece 202. Nanoparticles are put into the through hole 102. The surface of the particles is fixed with specific antibodies or specific antigens for specific detection of antigens or antibodies. In other implementations, micron particles can also be used, which will not be elaborated here. During use, the sample, elution buffer, secondary antibody, elution buffer and substrate are added to the outward expanding cone 204 in sequence. After a period of reaction, an optical fiber light source is activated by the external control system, and the chemiluminescence or fluorescence signal collected by another optical fiber is detected at the same time to detect biomolecular markers.

[0024] The working principle and usage process of this utility model are as follows: During use, the mesh plate 5 and filter membrane 4 are sequentially placed into the through hole 102. Then, a certain pushing force is manually applied to the outer tube 2 and the outlet tube 3, causing the outer tube 2 and the outlet tube 3 to compress the elastic extrusion plate 105 and insert it into the insertion groove 101. The frictional force applied by the elastic extrusion plate 105 fixes the outer tube 2 and the outlet tube 3 inside the insertion groove 101. Nanoparticles are placed into the through hole 102. Specific antibodies or specific antigens are fixed on the surface of the particles for specific detection of antigens or antibodies. Samples, elution buffer, secondary antibody, elution buffer, and substrate are sequentially added to the outward expanding cone 204. After a period of reaction, two optical fibers are inserted into the inner tube 201. An external control system activates one optical fiber light source, simultaneously detecting the chemiluminescence or fluorescence signal collected by the other optical fiber, thereby detecting biomolecular markers.

[0025] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A rapid detection device for biomolecular markers, comprising a main body (1), characterized in that: The main body (1) has several symmetrical insertion slots (101) on its upper and lower surfaces. The main body (1) has a through hole (102) inside, which connects the upper and lower insertion slots (101). The inner wall of the insertion slot (101) has an installation slot (104). An elastic extrusion piece (105) is fixedly connected in the installation slot (104). An outer tube (2) is inserted into the upper insertion slot (101), and an outlet tube (3) is inserted into the lower insertion slot (101). An inner tube (201) is provided inside the outer tube (2), and an outwardly expanding cone head (204) is fixedly connected to the upper end of the outer tube (2).

2. The rapid detection device for biomolecular markers according to claim 1, characterized in that: A limiting flange (103) is fixedly connected inside the through hole (102). A mesh plate (5) is placed inside the through hole (102) and above the limiting flange (103). A filter membrane (4) is placed inside the through hole (102) and above the mesh plate (5).

3. The rapid detection device for biomolecular markers according to claim 1, characterized in that: A connecting piece (202) is fixedly connected to the inner wall of the outer tube (2), and the other side of the connecting piece (202) is fixedly connected to the inner tube (201).

4. The rapid detection device for biomolecular markers according to claim 2, characterized in that: The outer tube (2) is fixedly connected to a pressing flange (203) below, and the pressing flange (203) cooperates with the limiting flange (103).

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