A disc spring preparation chromatographic column for industrial mass production

Abstract

The utility model discloses a disc spring preparation chromatographic column for industrialization mass production relates to chromatographic column technical field, specifically for a disc spring preparation chromatographic column for industrialization mass production, including mobile support and column pipe, mobile support top is equipped with mobile support fixed disc, and column pipe is equipped with column pipe fixed disc, and mobile support fixed disc is connected with column pipe fixed disc, the movable mounting of compression link is equipped in the column pipe, the compression link inner chamber is equipped with piston connecting rod, this disc spring preparation chromatographic column for industrialization mass production, install several disc spring pieces on piston connecting rod, use multiple disc spring piece superposition combination to provide elastic force, utilize disc spring piece to provide the axial compression function required by industrialization big diameter preparation chromatographic column, replace traditional high -pressure hydraulic oil's drive mode, at least reduce to the half of original equipment height, can avoid high -pressure hydraulic oil's leakage risk simultaneously, improve the use stability of chromatographic column.

Description

Technical Field

[0001] This utility model relates to the field of chromatography column technology, specifically to a disc spring chromatographic column for industrial mass production. Background Technology

[0002] Traditional large-diameter (generally exceeding 100 mm) preparative chromatographic columns employ Dynamic Axial Compression (DAC) technology to prevent bed collapse or eliminate the impact of packing material breakage. Conventional axial compression techniques struggle to produce high-efficiency columns. Only Dynamic Axial Compression (DAC) can achieve this; it is recognized as the only technique capable of maintaining good separation across various column sizes and is currently the best packing technique in preparative chromatography. Advanced DAC preparative column systems can self-pack, maintain column pressure, and self-unpack, combining the functions of a chromatographic column and a packer. Columns packed using DAC fully meet the requirements for bed continuity, homogeneity, stability, and compactness.

[0003] At the same time, the DAC dynamic axial compression preparation column system has a complex design, high manufacturing cost, and large space occupation. For sites with high cleanliness requirements, it also involves the risk of hydraulic oil leakage, posing a significant environmental risk when used in cleanrooms.

[0004] The dynamic axial compression column structure used in mass production is complex and bulky, lacks flexibility, is inconvenient to handle, and has poor process adaptability.

[0005] Traditional spring columns, with diameters of less than 100mm, are only suitable for laboratory use. Industrial mass production requires preparative chromatography column systems with larger diameters. Therefore, we designed a disc spring preparative chromatography column for industrial mass production. Utility Model Content

[0006] To address the shortcomings of existing technologies, this invention provides a disc spring preparative chromatographic column for large-scale industrial production, solving the problems mentioned in the background section.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a disc spring chromatographic column for industrial-scale production, comprising a movable support and a column tube, wherein the movable support is provided with a movable support fixing plate at its top, and the column tube is provided with a column tube fixing plate, and the movable support fixing plate is connected to the column tube fixing plate.

[0008] A compression rod is movably installed inside the column tube. A piston connecting rod is provided inside the compression rod. Several disc springs are installed on the piston connecting rod. A piston connecting plate is threaded onto the bottom end of the piston connecting rod. A piston is provided at the bottom end of the piston connecting plate. A protruding step is provided at one end of the piston connecting plate near the piston. A concave step is provided on the piston. The protruding step and the concave step are adapted to each other. The protruding step is installed into the inner cavity of the concave step to realize the connection between the piston connecting plate and the piston.

[0009] Preferably, the movable support fixing plate and the column tube fixing plate are fixed together by bolts.

[0010] Preferably, the column tube is provided with a column head flange, which is installed to the top of the column tube by a column tube retaining ring.

[0011] Preferably, the outer side of the tail of the column tube is provided with an annular groove, and a quick-connect chain for the tail of the column tube is provided on the outer side of the tail of the column tube and outside the annular groove. The annular groove is adapted to the quick-connect chain for the tail of the column tube, and a tail flange is provided at the bottom end of the column tube. The tail flange is connected to the column tube through the quick-connect chain for the tail of the column tube.

[0012] Preferably, a guide ring is sleeved on the middle of the outer side of the compression rod, the outer wall of the guide ring is in contact with the inner wall of the column tube, and a fastening flange is installed at the end of the compression rod, and the fastening flange is connected to the column head flange by bolts.

[0013] Preferably, a retaining ring is fixedly connected to the surface of the column tube.

[0014] Preferably, the bottom of the movable support is provided with self-locking casters.

[0015] This invention provides a disc spring preparative chromatographic column for large-scale industrial production, which has the following characteristics:

[0016] Beneficial effects:

[0017] 1. This disc spring preparation chromatography column for large-scale industrial production uses several disc springs installed on the piston connecting rod. The multiple disc springs are stacked and combined to provide elastic force. The disc springs provide the axial compression function required for industrial large-diameter preparation chromatography columns, replacing the high-pressure hydraulic oil drive mode. This reduces the height of the equipment to at least half of the original height, while avoiding the risk of high-pressure hydraulic oil leakage and improving the stability of the chromatography column.

[0018] 2. This disc spring-loaded chromatographic column for industrial mass production utilizes a convex step design on the piston connecting plate and a concave step design on the piston. The convex step is installed into the inner cavity of the concave step to achieve a quick and convenient connection between the piston connecting plate and the piston. After aligning the convex step with the concave step, rotating 90° completes the connection and installation. To disassemble, continue rotating 90° to separate the piston connecting plate and the piston. This facilitates practical applications in industrial mass production and improves the ease of column assembly.

[0019] 3. This disc spring preparative chromatography column for industrial-scale production, by adding a movable support, can be directly connected to the column packer, or easily transferred to different work sites after column packing, fully adapting to different application scenarios and improving the practicality of this disc spring preparative chromatography column for industrial-scale production. Attached Figure Description

[0020] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0021] Figure 2 This is a structural schematic diagram from another perspective of the present invention;

[0022] Figure 3 This is a schematic diagram of the front structure of this utility model;

[0023] Figure 4 This is a cross-sectional structural diagram of the column tube of this utility model;

[0024] Figure 5 This is a schematic diagram of the front of the column tube of this utility model.

[0025] In the diagram: 1. Movable support; 2. Movable support fixing plate; 3. Column tube fixing plate; 4. Column tube; 5. Column head flange; 6. Fastening flange; 7. Compression rod; 8. Bag fixing ring; 9. Column tail quick-release chain; 10. Column tube retaining ring; 11. Guide ring; 12. Disc spring; 13. Piston connecting plate; 14. Piston; 15. Column tail flange; 16. Piston connecting rod. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0027] Example 1: Refer to Figures 1-5This utility model provides a disc spring-loaded chromatographic column for industrial mass production, comprising a movable support 1 and a column tube 4. The bottom of the movable support 1 is equipped with self-locking casters for moving the movable support 1, and the top of the movable support 1 is provided with a movable support fixing plate 2. The column tube 4 is provided with a column tube fixing plate 3, and the movable support fixing plate 2 is connected to the column tube fixing plate 3.

[0028] A compression rod 7 is movably installed inside the column tube 4. A piston connecting rod 16 is located inside the compression rod 7, and several disc springs 12 are mounted on the piston connecting rod 16. Multiple disc springs 12 are stacked and combined to provide elastic force. The disc springs 12 provide the axial compression function required for industrial large-diameter preparative chromatography columns, replacing the high-pressure hydraulic oil drive mode. Furthermore, the load-deformation relationship of the disc springs is linearly adjustable, the preload compensation is effective and stable, the deformation energy per unit volume is large, and the disc springs have stronger buffering and shock absorption capabilities, making them suitable for high-load applications requiring precise preload or anti-loosening. Preparative chromatography columns are used in… During use, the packing may break or collapse, requiring precise adjustment and stabilization of the column bed. Using disc springs to prevent the column bed from loosening perfectly utilizes the characteristics of disc springs. The bottom end of the piston connecting rod 16 is threaded with a piston connecting plate 13. The bottom end of the piston connecting plate 13 is provided with a piston 14, and the piston connecting plate 13 is provided with a protruding step near the piston 14. The piston 14 is provided with a concave step, and the protruding step and the concave step are adapted to each other. The protruding step is installed into the inner cavity of the concave step to realize the connection between the piston connecting plate 13 and the piston 14.

[0029] The movable support fixing plate 2 and the column tube fixing plate 3 are fixed together by bolts. The column tube 4 is equipped with a column head flange 5. The column head flange 5 is installed to the top of the column tube 4 by a column tube retaining ring 10. The column tube 4 is provided with a column tube retaining plate for the column head flange 5.

[0030] An annular groove is provided on the outer side of the bottom end of the column tube 4, and a quick-connect chain 9 is provided on the outer side of the bottom end of the column tube 4 and outside the annular groove. The annular groove is compatible with the quick-connect chain 9. A column tail flange 15 is provided at the bottom end of the column tube 4, and the column tail flange 15 is installed at the bottom end of the column tube 4 via the quick-connect chain 9.

[0031] A guide ring 11 is sleeved on the middle of the outer side of the compression rod 7. The outer wall of the guide ring 11 contacts the inner wall of the column tube 4. The guide ring 11 ensures that the compression rod 7 and the piston connecting rod 16 are in the vertical direction, thereby ensuring that the elastic force of the disc spring 12 is always kept in the vertical direction.

[0032] The end of the compression rod 7 is fitted with a fastening flange 6, and the fastening flange 6 is connected to the column head flange 5 by bolts.

[0033] The surface of the column tube 4 is fixedly connected with a bag fixing ring 8 for fixing the packing bag.

[0034] In summary, for the disc spring preparative chromatography column used in large-scale industrial production, the installation process begins by aligning the column tube fixing plate 3 on the column tube 4 with the movable support fixing plate 2 on the movable support 1, and then connecting the column tube fixing plate 3 and the movable support fixing plate 2 using bolts. Finally, the column head flange 5 is fixed to the end of the column tube 4 using the column tube retainer 10.

[0035] Next, place the tail flange 15 at the tail end of the column tube 4, and use the tail quick-release chain 9 to fix the tail flange 15 to the tail of the column tube 4. Pass the piston connecting rod 16 through the compression rod 7, and install the required number of disc springs 12 into the head of the piston connecting rod 16. Then, fix the piston connecting plate 13 to the head of the piston connecting rod 16 and rotate the threaded connection. At this time, the compression rod 7, piston connecting rod 16, disc springs 12 and piston connecting plate 13 are assembled together.

[0036] After aligning the convex step on the piston connecting plate 13 with the concave step on the piston 14, rotate 90° to connect them. If disassembly is required, continue rotating 90° to separate the piston connecting plate 13 and the piston 14. Before installing the packing, a conduit needs to be connected to the corresponding orifice on the piston 14. Then, pass the conduit through the piston connecting rod 16 and the compression rod 7 in sequence, and connect it to the outlet of the external infusion pump.

[0037] Next, screw the guide ring 11 into the middle position of the compression rod 7 to ensure that the compression rod 7 is always in a vertically downward compressed state during the column loading process and always maintains coaxiality with the column tube 4. Then screw the fastening flange 6 into the head of the compression rod 7 to complete the assembly of the disc spring preparation chromatography column.

[0038] For the chromatographic packing operation, first push the column tube 4, with the column tail flange 15 already installed, together with the movable support 1, onto the column packer and connect and secure it.

[0039] Then, hang the assembled compression rod 7, fastening flange 6, guide ring 11, piston connecting rod 16, disc spring 12, piston connecting plate 13, and piston 14 together onto the central shaft of the column loading machine.

[0040] Beforehand, thoroughly mix the chromatographic packing material and homogenizing solvent according to the specified ratio, and then transfer the homogenizing solution into column tube 4.

[0041] Start the column loading machine. The central shaft of the column loading machine presses down, driving the compression rod 7 to move vertically downwards and make close contact with the disc spring 12. It continues to move downwards, squeezing the disc spring 12, piston connecting plate 13, and piston 14 in sequence. The piston 14 moves downwards to squeeze the homogenized solution and discharge the homogenized solvent. The remaining filler is gradually compressed to form a packing column bed. Continue to maintain the compression pressure value and stabilize the column bed for about 1 hour. Then, screw the fastening flange 6 downwards and align it with the column head flange 5 before connecting and fixing it with bolts.

[0042] Disconnect the connection between the column packer's central shaft and the compression rod 7, and the packed disc spring preparative chromatographic column can be removed from the column packer, completing the entire column packing process.

[0043] If it is necessary to change the packing material, the disc spring preparative chromatography column, along with the movable support 1, must first be pushed onto the column packer and fixedly connected.

[0044] Start the column loading machine. The central shaft of the column loading machine presses down, slightly pressing down on the compression rod 7, so that the connecting bolts between the fastening flange 6 and the column head flange 5 can be easily removed.

[0045] Slowly raise the central shaft of the column loading machine, disconnect the central shaft from the compression rod 7, remove the fastening flange 6 from the compression rod, and then reconnect the compression rod 7 to the central shaft of the column loading machine.

[0046] Loosen the quick-connect chain 9 at the column tail, remove the column tail flange 15, insert the end of the column tube 4 into the bag with the packing bag opening facing upwards, and secure the packing bag to the bag fixing ring 8.

[0047] Start the column loading machine, compress the compression rod 7 downwards, squeeze the piston 14, and completely drive the packing from the tail of the column tube 4 into the packing bag, thus completing the packing unloading work.

[0048] Finally, the following points should be noted: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installation", "connection", and "linkage" should be interpreted broadly, and can be mechanical or electrical connections, or internal connections between two components, or direct connections. "Up", "down", "left", "right", etc. are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may change.

[0049] Secondly: The accompanying drawings of the embodiments disclosed in this utility model only involve the structures involved in the embodiments disclosed in this utility model. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of this utility model can be combined with each other.

[0050] Finally: The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A disc spring preparative chromatography column for industrial-scale production, comprising a movable support (1) and a column tube (4), characterized in that: The movable support (1) is provided with a movable support fixing plate (2) at its top, and a column tube fixing plate (3) is provided on the column tube (4), and the movable support fixing plate (2) is connected to the column tube fixing plate (3). A compression rod (7) is movably installed inside the column tube (4). A piston connecting rod (16) is provided in the inner cavity of the compression rod (7). Several disc springs (12) are installed on the piston connecting rod (16). A piston connecting plate (13) is threadedly installed at the bottom end of the piston connecting rod (16). A piston (14) is provided at the bottom end of the piston connecting plate (13). A protruding step is provided at one end of the piston connecting plate (13) near the piston (14). A concave step is provided on the piston (14). The protruding step and the concave step are adapted to each other.

2. The disc spring preparative chromatographic column for industrial-scale production according to claim 1, characterized in that: The movable support fixing plate (2) and the column tube fixing plate (3) are fixed together by bolts.

3. The disc spring preparative chromatographic column for industrial-scale production according to claim 1, characterized in that: The column tube (4) is provided with a column head flange (5), which is installed to the top of the column tube (4) by a column tube retaining ring (10).

4. A disc spring preparative chromatographic column for industrial-scale production according to claim 1, characterized in that: The column tube (4) has an annular groove on the outer side of its tail, and a quick-release chain (9) is provided on the outer side of the tail of the column tube (4) and outside the annular groove. The annular groove is adapted to the quick-release chain (9), and a tail flange (15) is provided at the bottom end of the column tube (4). The tail flange (15) is connected to the column tube (4) through the quick-release chain (9).

5. A disc spring preparative chromatographic column for industrial-scale production according to claim 1, characterized in that: The compression rod (7) is fitted with a guide ring (11) on the middle of its outer side. The outer wall of the guide ring (11) is in contact with the inner wall of the column tube (4). The end of the compression rod (7) is fitted with a fastening flange (6), and the fastening flange (6) is connected to the column head flange (5) by bolts.

6. A disc spring preparative chromatographic column for industrial-scale production according to claim 1, characterized in that: The column tube (4) is fixedly connected to a bag fixing ring (8).

7. A disc spring preparative chromatographic column for industrial-scale production according to claim 1, characterized in that: The bottom of the movable support (1) is equipped with self-locking casters.

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