A chromatography device with controllable flow rate and time

Abstract

The utility model discloses a kind of chromatography devices of flow rate and time controllable, belong to medical instrument technical field, including liquid storage bottle, the upper end of liquid storage bottle is provided with inlet, the lower end of liquid storage bottle is provided with pipeline one, the lower end of pipeline one is provided with microfluid pump, the lower end of microfluid pump is provided with chromatography column, the lower end of chromatography column is provided with pipeline two, the lower end of pipeline two is provided with detector, the utility model is by being provided with the structure that liquid flow rate and reaction time can be controlled, the accurate control of liquid flow is realized, controller can automatically adjust the speed of microfluid pump and electromagnetic valve start-stop time according to preset experimental parameter, ensure that liquid flows at the speed set, and complete reaction within predetermined time, this design improves the accuracy and repeatability of experiment, especially suitable for the chromatography experiment that needs accurate control flow rate and time, can effectively improve experimental efficiency and the reliability of data.

Description

Technical Field

[0001] This utility model belongs to the field of medical device technology, specifically relating to a chromatography device with controllable flow rate and time. Background Technology

[0002] Liquid biopsy is an emerging diagnostic tool that enables early cancer screening by detecting potential biomarkers such as circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and extracellular vesicles (EVs) in the blood. In non-invasive screening for gastric cancer, this technique utilizes capillary action and negative pressure to extract a small amount of peripheral blood using a microneedle aspiration device. This method requires only 5–10 ml of blood from the patient and can obtain information covering cancer diagnosis and treatment by detecting the quantity of biomarkers and genetic characteristics in the blood.

[0003] However, in actual use, the chromatography device cannot control the flow rate and reaction time of the liquid when performing biopsies, which will affect the final data of the liquid during detection. Secondly, after the liquid is biopsied, the detection stage is exposed, which is prone to bacterial growth, and the closed detection stage is relatively inconvenient to clean. Utility Model Content

[0004] To address the problems mentioned in the background section, this invention provides a flow rate and time-controllable chromatography device. This device allows for control of the flow rate and reaction time during liquid biopsy and enables the detection stage to be sealed to prevent bacterial growth.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a chromatography device with controllable flow rate and time, comprising a storage bottle, an inlet at the upper end of the storage bottle, a first pipe at the lower end of the storage bottle, a microfluidic pump at the lower end of the first pipe, a chromatography column at the lower end of the microfluidic pump, a second pipe at the lower end of the chromatography column, a detector at the lower end of the second pipe, a controller at the lower side of the detector, a detection platform inside the detector, a sealing plate fixing mechanism on the outer side of the detector, and a solenoid valve on the side of the second pipe.

[0006] Preferably, the sealing plate fixing mechanism includes a sealing plate body, a groove, a fixing hole one, a fixing hole two, and a fixing component. The sealing plate body is slidably connected to the side of the detector. A groove is opened at the upper end of the side of the sealing plate body. Fixing holes one are opened at the upper ends of the other two sides of the sealing plate body. Fixing holes two are opened at the lower ends of the other two sides of the sealing plate body. A fixing component is provided inside the upper end of the side of the detector.

[0007] Preferably, the fixing assembly includes a pull rod, a pull ring, a spring, a limiting plate, a fixing rod, and a circular groove. A circular groove is provided inside the upper side of the detector. A limiting plate is slidably connected inside the circular groove. A fixing rod is provided on one side of the limiting plate, and a pull rod is provided on the other side of the limiting plate. A pull ring is provided at the other end of the pull rod, and a spring is sleeved on the surface of the pull rod inside the circular groove.

[0008] Preferably, the controller is connected to the microfluidic pump via a communication interface and can send control signals to adjust the flow rate of the microfluidic pump. The controller has a built-in flow rate control algorithm that can automatically adjust the rotation speed of the microfluidic pump according to preset experimental parameters, thereby achieving precise control of the liquid flow rate. The user can input the required flow rate value through the controller's interface, and the controller converts the value into a corresponding control signal and sends it to the microfluidic pump to ensure that the liquid flows at the set speed.

[0009] Preferably, the controller is connected to the solenoid valve via a communication interface and can send control signals to control the start and stop of the solenoid valve. The controller has a built-in time control module, which can automatically control the opening and closing of the solenoid valve according to preset time parameters. The user can input the required delay time and reaction time through the controller interface. The controller converts these parameters into corresponding control signals and sends them to the solenoid valve, thereby achieving precise time control of liquid flow.

[0010] Compared with the prior art, the beneficial effects of this utility model are:

[0011] 1. This utility model, by setting up a sealing plate fixing mechanism, can quickly and firmly fix the sealing plate body, ensuring the sealing performance of the detector. The groove design facilitates the manual installation and disassembly of the sealing plate body. Fixing hole one and fixing hole two cooperate with the fixing components to provide a stable fixing effect and prevent the sealing plate from loosening during use. This design not only improves the sealing performance and stability of the equipment, but also facilitates maintenance and cleaning, and extends the service life of the equipment.

[0012] 2. This utility model achieves precise control of liquid flow by setting up a structure that can control the liquid flow rate and reaction time. The controller can automatically adjust the speed of the microfluidic pump and the start and stop time of the solenoid valve according to the preset experimental parameters to ensure that the liquid flows at the set speed and completes the reaction within the predetermined time. This design improves the accuracy and repeatability of the experiment and is particularly suitable for chromatography experiments that require precise control of flow rate and time, which can effectively improve experimental efficiency and data reliability. Attached Figure Description

[0013] Figure 1 This is a perspective view of the present utility model;

[0014] Figure 2 This is a perspective view of the sealing plate fixing mechanism of this utility model;

[0015] Figure 3 This is an enlarged view of the fixing component of this utility model;

[0016] In the diagram: 1. Storage bottle; 2. Inlet; 3. Pipeline 1; 4. Microfluidic pump; 5. Chromatography column; 6. Pipeline 2; 7. Detector; 8. Controller; 9. Sealing plate fixing mechanism; 91. Sealing plate body; 92. Pull groove; 93. Fixing hole 1; 94. Fixing hole 2; 95. Fixing assembly; 951. Pull rod; 952. Pull ring; 953. Spring; 954. Limiting plate; 955. Fixing rod; 956. Circular groove; 10. Solenoid valve; 11. Detection stage. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Please see Figure 1-3 The present invention provides the following technical solution: a chromatography device with controllable flow rate and time, comprising a storage bottle 1, an inlet 2 at the upper end of the storage bottle 1, a pipe 3 at the lower end of the storage bottle 1, a microfluidic pump 4 at the lower end of the pipe 3, a chromatography column 5 at the lower end of the microfluidic pump 4, a pipe 6 at the lower end of the chromatography column 5, a detector 7 at the lower end of the pipe 6, a controller 8 at the lower end of the side of the detector 7, a detection platform 11 inside the detector 7, a sealing plate fixing mechanism 9 on the outside of the detector 7, and a solenoid valve 10 on the side of the pipe 6.

[0019] Specifically, the sealing plate fixing mechanism 9 includes a sealing plate body 91, a groove 92, a first fixing hole 93, a second fixing hole 94, and a fixing component 95. The sealing plate body 91 is slidably connected to the side of the detector 7. The upper side of the sealing plate body 91 has a groove 92. The upper sides of the other two sides of the sealing plate body 91 have first fixing holes 93. The lower sides of the other two sides of the sealing plate body 91 have second fixing holes 94. The fixing component 95 is installed inside the upper side of the detector 7.

[0020] By adopting the above technical solution, the sealing plate fixing mechanism 9 can quickly and firmly fix the sealing plate body 91, ensuring the sealing of the detector 7. The design of the groove 92 facilitates the manual installation and disassembly of the sealing plate body 91. The fixing hole 1 93 and fixing hole 2 94 cooperate with the fixing component 95 to provide a stable fixing effect and prevent the sealing plate from loosening during use.

[0021] Specifically, the fixing component 95 includes a pull rod 951, a pull ring 952, a spring 953, a limiting plate 954, a fixing rod 955, and a circular groove 956. A circular groove 956 is formed inside the upper side of the detector 7. The limiting plate 954 is slidably connected inside the circular groove 956. A fixing rod 955 is provided on one side of the limiting plate 954, and a pull rod 951 is provided on the other side of the limiting plate 954. A pull ring 952 is provided at the other end of the pull rod 951. A spring 953 is fitted inside the circular groove 956 on the surface of the pull rod 951.

[0022] By adopting the above technical solution, the fixing component 95 utilizes the elasticity of the spring 953 to enable the fixing rod 955 to be stably inserted into the fixing hole 1 93 and the fixing hole 2 94, thereby achieving rapid fixing of the sealing plate body 91. The pull ring 952 facilitates the operator to pull the pull rod 951, thereby achieving rapid release of the fixing rod 955 and improving the maintenance and cleaning efficiency of the equipment.

[0023] Specifically, the controller 8 is connected to the microfluidic pump 4 via a communication interface and can send control signals to adjust the flow rate of the microfluidic pump 4. The controller 8 has a built-in flow rate control algorithm that can automatically adjust the rotation speed of the microfluidic pump 4 according to preset experimental parameters, thereby achieving precise control of the liquid flow rate. The user can input the required flow rate value through the interface of the controller 8, and the controller 8 converts the value into a corresponding control signal and sends it to the microfluidic pump 4 to ensure that the liquid flows at the set speed.

[0024] By adopting the above technical solution, the controller 8 can accurately control the flow rate of the microfluidic pump 4 to meet the flow rate requirements under different experimental conditions. Users can easily set the flow rate parameters through the interface of the controller 8. The controller 8 automatically adjusts the rotation speed of the microfluidic pump 4 according to the preset algorithm to ensure that the liquid flows at the set speed, thereby improving the accuracy and repeatability of the experiment.

[0025] Specifically, the controller 8 is connected to the solenoid valve 10 via a communication interface and can send control signals to control the start and stop of the solenoid valve 10. The controller 8 has a built-in time control module, which can automatically control the opening and closing of the solenoid valve 10 according to preset time parameters. The user can input the required delay time and reaction time through the interface of the controller 8. The controller 8 converts these parameters into corresponding control signals and sends them to the solenoid valve 10, thereby achieving precise time control of liquid flow.

[0026] By adopting the above technical solution, the controller 8 can accurately control the start and stop time of the solenoid valve 10 to meet the time control requirements under different experimental conditions. Users can easily set the delay time and reaction time through the interface of the controller 8. The controller 8 automatically controls the opening and closing of the solenoid valve 10 according to the preset parameters to ensure accurate time control of liquid flow and improve the accuracy and repeatability of the experiment.

[0027] The microfluidic pump 4 in this invention is a previously disclosed technology, and the selected model is Harvard Apparatus PHD Ultra.

[0028] The detector 7 in this invention is based on existing publicly available technology, and the selected model is Agilent 1260 Infinity II UV.

[0029] The controller 8 in this invention is a publicly available technology, and the selected model is Arduino Mega 2560.

[0030] The solenoid valve 10 in this utility model is a publicly available technology, and the selected model is Solenoid Valve12VDC.

[0031] The working principle and usage process of this utility model are as follows: When using this utility model, the liquid to be processed is first added to the storage bottle 1 through the inlet 2. The required flow rate and time parameters are set by the controller 8. The controller 8 automatically adjusts the speed of the microfluidic pump 4 according to the preset algorithm to ensure that the liquid passes through the chromatography column 5 at the set speed for separation. At the same time, the controller 8 automatically controls the opening and closing of the solenoid valve 10 according to the preset time parameters to ensure precise time control of the liquid flow. The separated liquid enters the detector 7 through the pipe 6 for detection. The detection result is displayed on the detection stage 11. The sealing plate fixing mechanism 9 ensures the sealing of the detector 7 to prevent liquid leakage. This design not only improves the automation level of the chromatography device, but also improves the accuracy and repeatability of the experiment. It is particularly suitable for chromatography experiments that require precise control of flow rate and time.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A flow rate and time controllable chromatography device comprising a reservoir bottle (1), characterized in that: The upper end of the storage bottle (1) is provided with an inlet (2), the lower end of the storage bottle (1) is provided with a pipe (3), the lower end of the pipe (3) is provided with a microfluidic pump (4), the lower end of the microfluidic pump (4) is provided with a chromatography column (5), the lower end of the chromatography column (5) is provided with a pipe (6), the lower end of the pipe (6) is provided with a detector (7), the lower end of the side of the detector (7) is provided with a controller (8), the inside of the detector (7) is provided with a detection platform (11), the outside of the detector (7) is provided with a sealing plate fixing mechanism (9), and the side of the pipe (6) is provided with a solenoid valve (10).

2. The flow rate and time controllable chromatography device according to claim 1, characterized in that: The sealing plate fixing mechanism (9) includes a sealing plate body (91), a groove (92), a fixing hole one (93), a fixing hole two (94), and a fixing component (95). The side of the detector (7) is slidably connected to the sealing plate body (91). The upper side of the sealing plate body (91) is provided with a groove (92). The upper sides of the other two sides of the sealing plate body (91) are provided with fixing holes one (93). The lower sides of the other two sides of the sealing plate body (91) are provided with fixing holes two (94). The upper side of the detector (7) is provided with a fixing component (95).

3. The flow rate and time controllable chromatography device according to claim 2, characterized in that: The fixing component (95) includes a pull rod (951), a pull ring (952), a spring (953), a limiting plate (954), a fixing rod (955), and a circular groove (956). The detector (7) has a circular groove (956) inside its upper side. The limiting plate (954) is slidably connected inside the circular groove (956). A fixing rod (955) is provided on one side of the limiting plate (954), and a pull rod (951) is provided on the other side of the limiting plate (954). A pull ring (952) is provided at the other end of the pull rod (951). A spring (953) is sleeved inside the circular groove (956) on the surface of the pull rod (951).

4. The flow rate and time controllable chromatography device of claim 1, wherein: The controller (8) is connected to the microfluidic pump (4) through a communication interface and can send control signals to adjust the flow rate of the microfluidic pump (4). The controller (8) has a built-in flow rate control algorithm, which can automatically adjust the rotation speed of the microfluidic pump (4) according to the preset experimental parameters, thereby achieving precise control of the liquid flow rate. The user can input the required flow rate value through the interface of the controller (8), and the controller (8) converts the value into a corresponding control signal and sends it to the microfluidic pump (4) to ensure that the liquid flows at the set speed.

5. The flow rate and time controllable chromatography device of claim 1, wherein: The controller (8) is connected to the solenoid valve (10) through a communication interface and can send control signals to control the start and stop of the solenoid valve (10). The controller (8) has a built-in time control module, which can automatically control the opening and closing of the solenoid valve (10) according to the preset time parameters. The user can input the required delay time and reaction time through the interface of the controller (8). The controller (8) converts these parameters into corresponding control signals and sends them to the solenoid valve (10), thereby realizing precise time control of liquid flow.

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