A cell factory device

Abstract

The utility model relates to cell culture technical field discloses a kind of cell factory device, including plug body and the cover disc and culture tray of stacked arrangement, cover disc is located the top of culture tray, culture tray includes culture bottom plate, culture side plate and communicating part, culture side plate is arranged along the edge of culture bottom plate, and one end of culture side plate is connected with culture bottom plate and is enclosed to form culture cavity, and the other end of culture side plate is connected with culture tray, and culture tray seals culture cavity, culture bottom plate is equipped with through-hole, and communicating part is located in culture cavity, and one end of communicating part is connected in through-hole, and the other end of communicating part is formed with flow port between culture tray, cover disc is equipped with liquid injection hole, and the position of liquid injection hole is correspondingly set with the position of communicating part, and liquid injection hole is communicated with culture cavity by flow port, and plug body is sealedly connected with one end of communicating part away from cover disc.The cell factory device of the utility model improves the control of flatness of cell factory device, and ensures that cell growth is evenly distributed.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a cell factory device. Background Technology

[0002] Existing cell factory devices consist of a cover plate, an intermediate layer, and a culture substrate, which are stacked sequentially. Each of these components requires three corresponding molds for injection molding. Due to the large number of injection molds, significant cumulative assembly errors occur after assembly, making it impossible to guarantee the flatness of each layer of the cell factory device. This results in uneven cell growth distribution, or even areas where no cells grow. Utility Model Content

[0003] The aim is to solve at least one of the technical problems existing in the prior art. This utility model provides a cell factory device that improves the control of the flatness of the cell factory device and ensures uniform cell growth distribution.

[0004] To achieve the above objectives, this utility model provides a cell factory device, including a plug and stacked cover plates and culture trays. The cover plate is located above the culture trays. The culture tray includes a culture base plate, culture side plates, and a connecting portion. The culture side plates are disposed along the edge of the culture base plate. One end of the culture side plate is connected to the culture base plate to form a culture cavity. The other end of the culture side plate is connected to the culture tray. The culture tray seals the culture cavity. The culture base plate has a through hole. The connecting portion is located inside the culture cavity. One end of the connecting portion is connected to the through hole. The other end of the connecting portion forms a flow outlet with the culture tray. The cover plate has an injection hole. The position of the injection hole corresponds to the position of the connecting portion. The injection hole communicates with the culture cavity through the flow outlet. The plug is sealed to the end of the connecting portion away from the cover plate.

[0005] As a preferred embodiment, the culture trays are provided in multiple ways, and the multiple culture trays are stacked and connected. Adjacent culture trays are connected through the connecting portion. The plug is connected to the culture tray furthest from the cover plate. One end of the connecting portion has the flow port between it and another culture tray.

[0006] As a preferred embodiment, the plug body includes a plug body, one end of which protrudes outward to form a snap ring, the snap ring snapping with the end of the connecting portion facing the cover plate, and the edge of the other end of the plug body is provided with a notch and a snap portion, the snap portion protruding outward along the periphery, the snap portion snapping with the end of the connecting portion away from the cover plate.

[0007] As a preferred embodiment, the plug body further includes an elastic ring, which is sleeved on the outer peripheral surface of the plug body and has an interference fit with the inner peripheral wall of the communicating portion.

[0008] As a preferred embodiment, the bottom surface of the culture base plate opposite to the culture chamber is connected to a plurality of support protrusions, and the height a of the support protrusions is less than the height b of the culture chamber.

[0009] As a preferred embodiment, the end of the culture side plate facing the cover plate is provided with a positioning groove, the shape of which corresponds to the bottom shape of the cover plate and the shape of the culture base plate.

[0010] As a preferred embodiment, the positioning groove is provided with a connecting surface facing the cover plate, the connecting surface is provided with a welding protrusion facing the cover plate, the cover plate is provided with a first welding groove corresponding to the position, the welding protrusion is connected to the first welding groove, and one side of the lower surface of the culture base plate is provided with a second welding groove for connecting with the welding protrusion, the second welding groove is positioned corresponding to the welding protrusion.

[0011] As a preferred embodiment, the two sides of the welding protrusion are contact planes, and the contact planes are provided with concave and convex corrugations.

[0012] As a preferred embodiment, the bottom surface of the cover plate is provided with scale lines, and the scale lines are integrally formed with the cover plate.

[0013] As a preferred embodiment, the diameter of the injection hole is set at 11mm-12mm.

[0014] Compared with the prior art, the cell factory device of this utility model has the following advantages: a cover plate is placed on top of a culture dish, and the edge of the cover plate is connected to the edge of the culture dish to achieve a seal on the culture dish. The cover plate has an injection hole for injecting culture medium into the culture dish, and the injection hole can cooperate with the cover to achieve a seal. The culture dish includes a culture base plate, culture side plates, and a connecting portion. The culture side plates are connected to the culture base plate and enclose a culture chamber for cell culture. The culture base plate has a through hole, and the connecting portion is connected to the through hole. The connecting portion is located inside the culture chamber and protrudes from the culture base plate, so that the culture medium can be retained in the culture chambers of each layer after injection. When injecting the culture medium, the culture medium is injected into the culture chamber through the injection hole and the flow outlet. A plug is connected to the end of the connecting portion away from the cover plate to achieve a seal on the culture dish. This invention requires only two sets of molds for the cover plate and the culture plate during injection molding, reducing production costs. At the same time, the bottom culture plate, combined with the plug, forms a base, avoiding the need to weld the culture plate to the plate produced by another mold, reducing cumulative assembly errors, thereby improving the control of the flatness of the cell factory device and ensuring uniform cell growth distribution. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present utility model.

[0016] Figure 2 This is a schematic diagram of the structure of an embodiment of the present invention when there is only one culture tray.

[0017] Figure 3 This is a schematic diagram of the component disassembly structure of an embodiment of this utility model.

[0018] Figure 4 This is a schematic diagram of the plug body in an embodiment of the present invention.

[0019] Figure 5 This is a schematic diagram of the component disassembly structure of the plug body according to an embodiment of the present invention.

[0020] Figure 6 This is a cross-sectional view of the plug body according to an embodiment of the present invention.

[0021] Figure 7 This is a top view of the overall structure of an embodiment of this utility model.

[0022] Figure 8 This is an embodiment of the present utility model. Figure 7 Cross-sectional view at point DD.

[0023] Figure 9 This is an embodiment of the present utility model. Figure 8 A magnified structural diagram of point A in the middle.

[0024] Figure 10 This is an embodiment of the present utility model. Figure 9 A magnified structural diagram at point B in the middle.

[0025] Figure 11 This is an embodiment of the present utility model. Figure 9 A magnified structural diagram at point C.

[0026] Figure 12 This is a schematic diagram of the structure of the cover plate in an embodiment of this utility model.

[0027] Figure 13 This is a schematic diagram of the structure of the culture tray in an embodiment of this utility model.

[0028] Figure 14 This is a schematic diagram of the bottom structure of the culture tray in an embodiment of this utility model.

[0029] In the picture:

[0030] 10. Plug body; 11. Plug body; 12. Snap-fit ​​ring; 13. Notch; 14. Snap-fit ​​part; 15. Elastic ring;

[0031] 20. Cover plate; 21. First welding groove; 22. Scale line; 23. Injection hole;

[0032] 30. Culture tray; 31. Culture base plate; 32. Second welding groove; 33. Support boss; 34. Through hole; 35. Culture side plate; 36. Positioning groove; 37. Connecting surface; 38. Welding protrusion; 39. Contact plane; 40. Connecting part; 41. Culture chamber; 42. Flow outlet; 43. Marking part;

[0033] 50. Gas-liquid pipeline; 51. Sealing cap; 52. Vent cap. Detailed Implementation

[0034] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit its scope.

[0035] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and "outer" used to indicate the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0036] In the description of this utility model, it should be understood that the terms "connected," "linked," and "fixed," etc., used in this utility model should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or a welded connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly defined. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0037] like Figures 1 to 14As shown, a preferred embodiment of the cell factory device of this utility model includes a plug 10 and a stacked cover plate 20 and a culture tray 30. The cover plate 20 is located above the culture tray 30. The culture tray 30 includes a culture base plate 31, a culture side plate 35, and a connecting portion 40. The culture side plate 35 is arranged along the edge of the culture base plate 31. One end of the culture side plate 35 is connected to the culture base plate 31 to form a culture cavity 41. The other end of the culture side plate 35 is connected to the culture tray 30. The culture tray 30 seals the culture cavity 41. The culture base plate 31 is provided with a through hole 34. The connecting portion 40 is located in the culture cavity 41. One end of the connecting portion 40 is connected to the through hole 34. The other end of the connecting portion 40 and the culture tray 30 form a flow outlet 42. The cover plate 20 is provided with an injection hole 23. The position of the injection hole 23 corresponds to the position of the connecting portion 40. The injection hole 23 communicates with the culture cavity 41 through the flow outlet 42. The plug 10 is sealed to the end of the connecting portion 40 away from the cover plate 20.

[0038] The cell factory apparatus of this invention includes a cover plate 20 placed over a culture tray 30, with its edge connected to the edge of the culture tray 30 for sealing the culture tray 30 from above. The cover plate 20 has an injection hole 23 for injecting culture medium into the culture tray 30, and the injection hole 23 cooperates with the cover to achieve a seal. The culture tray 30 includes a culture base plate 31, culture side plates 35, and a connecting portion 40. The culture side plates 35 are connected to the culture base plate 31 and enclose a culture chamber 41 for cell culture. The culture base plate 31 has a through hole 34, and the connecting portion 40 is connected to the through hole 34. The connecting portion 40 is located within the culture chamber 41 and protrudes from the culture base plate 31, allowing the culture medium to be retained in each layer of the culture chamber 41 after injection. During injection of culture medium, the culture medium is injected into the culture chamber 41 through the injection hole 23 and the flow outlet 42. The plug 10 is connected to the end of the connecting portion 40 away from the cover plate 20 to achieve a seal on the culture tray 30. This invention requires only two sets of molds for the cover plate 20 and the culture tray 30 during injection molding, reducing production costs. Simultaneously, the bottommost culture tray 30, combined with the plug 10, forms a base, avoiding the need to weld the culture tray 30 to a tray produced by another mold, reducing accumulated assembly errors, and thus improving the control over the flatness of the cell factory device, ensuring uniform cell growth distribution.

[0039] As one embodiment, such as Figures 1 to 3 As shown, the injection port 23 is threaded, allowing it to mate with a threaded cap. The cap can be a vent cap 52 or a sealing cap 51, enabling either ventilation or sealing in the cell factory. Alternatively, the injection port 23 can be connected to the gas-liquid tubing 50 via threads, allowing for direct aseptic liquid transfer and efficient, rapid culture and amplification. The vent cap 52, sealing cap 51, and gas-liquid tubing 50 are existing technologies and will not be described further here.

[0040] As one embodiment, such as Figure 9 As shown, the height H of the flow port 42 is set at 2mm-3mm. Preferably, the height H of the flow port 42 is set at 2.5mm. When liquid enters the cell factory, it can be evenly distributed in each culture tray 30 through the flow port 42.

[0041] In one embodiment, the thickness of the cover plate 20 and the culture plate 30 is set at 2.8 mm to meet the requirements of injection molding and support strength.

[0042] Furthermore, such as Figure 1 or Figure 3 As shown, multiple culture trays 30 are provided, stacked and connected together. Adjacent culture trays 30 are connected through a connecting part 40. A plug 10 is connected to the culture tray 30 furthest from the cover plate 20. One end of the connecting part 40 has a flow port 42 between it and another culture tray 30. The culture base plate 31 of the culture tray 30 is connected to the culture side plate 35 of the next culture tray 30 to achieve the stacking connection of multiple culture trays 30, thereby expanding the culture space and meeting different cell culture needs. The plug 10 is connected to the culture tray 30 furthest from the cover plate 20 to achieve the sealing of the culture tray 30. Culture medium is injected into adjacent culture trays 30 through the flow port 42, and adjacent culture trays 30 are connected through the connecting part 40.

[0043] As one embodiment, such as Figure 1 As shown, the culture tray 30 can be set to single layer, double layer, 4 layers, 5 layers, 10 layers, 20 layers, 40 layers, etc., and the number of culture trays 30 can be set according to actual needs.

[0044] Furthermore, such as Figures 4 to 6 As shown, the plug body 10 includes a plug body 11. One end of the plug body 11 protrudes peripherally to form a snap-fit ​​ring 12, which snaps into the end of the connecting portion 40 facing the cover plate 20. The other end of the plug body 11 has a notch 13 and a snap-fit ​​portion 14. The snap-fit ​​portion 14 protrudes peripherally and snaps into the end of the connecting portion 40 away from the cover plate 20. The snap-fit ​​ring 12 and the snap-fit ​​portion 14 snap into both ends of the connecting portion 40 to achieve the connection between the plug body 11 and the connecting portion 40, preventing it from falling off during use. The notch 13 at one end of the plug body 11 provides a certain elastic deformation space during snap-fit, preventing damage to the plug body 10 during snap-fit.

[0045] As one embodiment, such as Figures 4 to 6 As shown, multiple notches 13 are provided and spaced apart circumferentially along the connecting portion 40 to provide a larger space for elastic deformation during snap-fitting, preventing damage to the plug 10 during snap-fitting.

[0046] As one embodiment, such as Figures 4 to 6 As shown, multiple snap-fit ​​portions 14 are provided, and notches 13 are located between adjacent snap-fit ​​portions 14. The snap-fit ​​portions 14 are snapped into the connecting portion 40, thereby improving the connection stability between the plug body 10 and the connecting portion 40.

[0047] Furthermore, such as Figures 4 to 6 As shown, the plug body 10 also includes an elastic ring 15, which is sleeved on the outer peripheral surface of the plug body 11. The elastic ring 15 is press-fitted with the inner peripheral wall of the connecting portion 40. The elastic ring 15 is located between the snap-fit ​​portion 14 and the snap-fit ​​ring 12, and is press-fitted with the inner peripheral wall of the connecting portion 40, thereby improving the sealing effect between the plug body 10 and the connecting portion 40.

[0048] As one embodiment, such as Figures 4 to 6 As shown, the plug body 11 is made of ABS material, and the elastic ring 15 is made of TPE material. ABS material has good impact resistance and chemical resistance, while TPE has good elasticity and adhesion, which allows the elastic ring 15 to be well wrapped and fused to the plug body 11 to form a whole.

[0049] As one embodiment, such as Figures 4 to 6 As shown, the culture tray 30 is generally glossy and transparent. The elastic ring 15 on the plug body 10 is press-fitted with the inner wall of the connecting part 40 by 0.15mm on one side. When the plug body 10 is installed on the bottommost culture tray 30, it can form a very good sealing effect and will not fall off or leak. The plug body 10 has 4 snap-fit ​​parts 14 and 4 U-shaped notches 13, which form effective elastic contraction when the plug body 10 is installed to prevent the culture tray 30 from being squeezed. The snap-fit ​​parts 14 are effectively snapped into one end of the connecting part 40 and will not fall off or loosen.

[0050] Furthermore, such as Figure 14 As shown, a plurality of support protrusions 33 are connected to the bottom surface of the culture base plate 31 opposite to the culture chamber 41. The height 'a' of the support protrusions 33 is less than the height 'b' of the culture chamber 41. The support protrusions 33 support the culture trays 30, reducing the support area of ​​the support surface and making it easier to control the flatness of the support surface. By controlling the support protrusions 33, the levelness can be adjusted so that the cell factory is in a flat state when placed on a horizontal platform, which is conducive to uniform cell growth and prevents local cell stagnation. When multiple culture trays 30 are stacked, the support protrusions 33 can be located within the culture chamber 41 after the culture trays 30 are stacked.

[0051] As one embodiment, such as Figure 14 As shown, three support bosses 33 are provided in a triangular arrangement to improve the stability of the support.

[0052] As one embodiment, such as Figure 14As shown, the height of the support boss 33 is set between 1mm and 3mm. Preferably, the support boss 33 is 1.5mm high, so that the support boss 33 can meet the support requirements and reduce the space occupied by the culture chamber 41.

[0053] As one embodiment, such as Figures 1 to 3 As shown, reinforcing ribs are provided on the outer periphery of the culture side plate 35 and the outer periphery of the injection hole 23 of the cover plate 20. The reinforcing ribs on the outer periphery of the injection hole 23 enhance the strength of the injection hole 23, compensating for the inherent low strength of the small diameter, and preventing the injection hole 23 from cracking when inserting or removing tubing and installing the cover. At the same time, the reinforcing ribs on the culture side plate 35 improve the supporting strength of the culture plate 30.

[0054] Furthermore, such as Figures 8 to 11 As shown, the end of the culture side plate 35 facing the cover plate 20 is provided with a positioning groove 36. The shape of the positioning groove 36 corresponds to the bottom surface shape of the cover plate 20 and the shape of the culture base plate 31. The positioning groove 36 in the culture side plate 35 is provided to facilitate the positioning of the stacked connection between the culture tray 30 and the cover plate 20, thereby improving the assembly stability of the stacked structure.

[0055] Furthermore, such as Figures 8 to 11 As shown, the positioning groove 36 has a connecting surface 37 facing the cover plate 20, and the connecting surface 37 has a welding protrusion 38 protruding towards the cover plate 20. The cover plate 20 has a corresponding first welding groove 21, and the welding protrusion 38 is connected to the first welding groove 21. The lower surface of the culture base plate 31 has a second welding groove 32 for connecting with the welding protrusion 38, and the second welding groove 32 is positioned corresponding to the welding protrusion 38. The welding protrusion 38 is ultrasonically welded in the first welding groove 21 and in the second welding groove 32. Ultrasonic welding is performed under pressure, where ultrasonic waves cause the material surfaces to rub against each other and generate heat, thereby melting and bonding the materials together at the joint. After welding, the ultrasonic waves melt the welding protrusion 38, firmly bonding the upper and lower layers together to achieve a fixed connection between the culture plates 30 and between the culture plates 30 and the cover plate 20.

[0056] Furthermore, such as Figures 8 to 11 As shown, the two sides of the welding protrusion 38 are contact planes 39, which are located on the connecting surface 37. That is, the contact plane 39 is part of the connecting surface 37. The contact plane 39 is provided with concave and convex corrugations, which increases the roughness and improves the ultrasonic welding effect. In addition, the ultrasonic debris generated during the welding process enters the corrugations and fuses, avoiding the debris from affecting the cleanliness and culture effect of the cell factory.

[0057] As one embodiment, such as Figures 8 to 11As shown, the welding bump 38 is triangular, with two protruding and intersecting welding surfaces set at an included angle of 70°, and the distance between the other ends of the intersecting welding surfaces is 0.85mm. The height of the welding bump 38 is set at 0.6mm, and the top of the intersecting welding surfaces is rounded to achieve better ultrasonic welding between the culture trays 30 and between the culture trays 30 and the cover plate 20.

[0058] Furthermore, such as Figure 7 and Figure 12 As shown, the bottom surface of the cover plate 20 has graduation lines 22, which are integrally formed with the cover plate 20. The cover plate 20 is injection molded with the graduation lines 22 built-in, eliminating the need for additional processing of the graduation lines 22. This allows for quick and intuitive observation of the liquid volume and precise control of the liquid volume. The open surface of the cover plate 20 allows for quick and effective observation of cell growth, enabling timely replenishment and replacement of liquid. During liquid addition, the cell factory is placed on its side, meaning the graduation lines 22 extend along the height direction, preventing the culture medium from filling the entire cell factory. If the cell factory were placed upright for liquid addition, the liquid volume in each layer would be uneven, and the actual liquid volume in each culture tray 30 would be unknown. After placing the cell factory on its side, add the predetermined amount of liquid through the injection hole 23. Under the influence of gravity, the culture medium will be evenly distributed in each layer, making the liquid volume in each layer consistent. At this time, it is easy to know whether the liquid volume added to each layer has reached the predetermined value through the scale line 22. When the liquid volume reaches the predetermined value, slowly stand the cell factory upright. The culture medium will flow in the current layer and fill each layer, which is convenient for cell adhesion culture.

[0059] Furthermore, such as Figure 8 As shown, the diameter of the injection well 23 is set at 11mm-12mm. The injection well 23 in the cell factory has a small diameter, with an inner diameter of 11mm-12mm. When used with sterile tubing, it minimizes the impact on cells and reduces the shear force generated by the disturbance of the culture medium during the addition and pouring of liquids, thus better protecting the cells.

[0060] As one embodiment, such as Figure 14 As shown, a marking part 43 is provided on the side of the culture base plate 31 facing away from the culture chamber 41. The marking part 43 can be flexibly changed to different requirements and can be designed according to different needs. The marking is designed on the edge side, so the liquid will not come into contact with this plane during cell factory culture, so it will not cause residue and will not affect the observation of cell growth.

[0061] In summary, this utility model embodiment provides a cell factory device. A cover plate 20 is placed over a culture tray 30, and the edge of the cover plate 20 is connected to the edge of the culture tray 30 to seal the culture tray 30 from above. The cover plate 20 has an injection hole 23 for injecting culture medium into the culture tray 30. The injection hole 23 can cooperate with the cover to seal the injection hole 23. The culture tray 30 includes a culture base plate 31, a culture side plate 35, and a connecting part 40. The culture side plate 35 is connected to the culture base plate 31 and surrounds it to form a culture cavity 41 for cell culture. The culture base plate 31 has a through hole 34, and the connecting part 40 is connected to the through hole 34. The connecting part 40 is located inside the culture cavity 41 and protrudes from the culture base plate 31 so that the culture medium can be retained in the culture cavities 41 after injection. When injecting culture medium, the culture medium is injected into the culture cavity 41 through the injection hole 23 and the flow port 42. The plug 10 is connected to the end of the connecting portion 40 away from the cover plate 20 to achieve a seal on the culture tray 30. This invention requires only two sets of molds for the cover plate 20 and the culture tray 30 during injection molding, reducing production costs. Simultaneously, the bottommost culture tray 30, combined with the plug 10, forms a base, avoiding the need to weld the culture tray 30 to a tray produced by another mold, reducing accumulated assembly errors, and thus improving the control over the flatness of the cell factory device, ensuring uniform cell growth distribution.

[0062] The above are merely preferred embodiments of this utility model. It should be noted that, for those skilled in the art, several improvements and substitutions can be made without departing from the technical principles of this utility model, and these improvements and substitutions should also be considered within the protection scope of this utility model.

Claims

1. A cell factory device, characterized in that: The device includes a plug and stacked cover plate and culture tray. The cover plate is located above the culture tray. The culture tray includes a culture base plate, a culture side plate, and a connecting portion. The culture side plate is disposed along the edge of the culture base plate. One end of the culture side plate is connected to the culture base plate to form a culture cavity. The other end of the culture side plate is connected to the culture tray. The culture tray seals the culture cavity. The culture base plate has a through hole. The connecting portion is located inside the culture cavity. One end of the connecting portion is connected to the through hole. The other end of the connecting portion forms a flow outlet with the culture tray. The cover plate has an injection hole. The position of the injection hole corresponds to the position of the connecting portion. The injection hole communicates with the culture cavity through the flow outlet. The plug is sealed to the end of the connecting portion away from the cover plate.

2. The cell factory apparatus according to claim 1, characterized in that: The culture trays are provided in multiple ways, and the multiple culture trays are stacked and connected. Adjacent culture trays are connected through the connecting part. The plug is connected to the culture tray furthest from the cover plate. One end of the connecting part has the flow port between it and another culture tray.

3. The cell factory device according to claim 1, characterized in that: The plug body includes a plug body, one end of which protrudes outward to form a snap ring. The snap ring snaps with the end of the connecting part facing the cover plate. The edge of the other end of the plug body is provided with a notch and a snap part. The snap part protrudes outward along the periphery and snaps with the end of the connecting part away from the cover plate.

4. The cell factory apparatus according to claim 3, characterized in that: The plug body also includes an elastic ring, which is sleeved on the outer peripheral surface of the plug body and is interference-fitted with the inner peripheral wall of the connecting portion.

5. The cell factory apparatus according to claim 1, characterized in that: The bottom surface of the culture base plate opposite to the culture chamber is connected to a plurality of support bosses, the height a of which is less than the height b of the culture chamber.

6. The cell factory apparatus according to claim 1, characterized in that: The culture side plate has a positioning groove at one end facing the cover plate, and the shape of the positioning groove corresponds to the shape of the bottom surface of the cover plate and the shape of the culture base plate.

7. The cell factory apparatus according to claim 6, characterized in that: The positioning groove has a connecting surface facing the cover plate, the connecting surface has a welding protrusion facing the cover plate, the cover plate has a first welding groove corresponding to the position, the welding protrusion is connected to the first welding groove, the lower surface of the culture base plate has a second welding groove for connecting with the welding protrusion, and the second welding groove is positioned corresponding to the welding protrusion.

8. The cell factory apparatus according to claim 7, characterized in that: The welding protrusion has two contact planes on both sides, and the contact planes are provided with concave and convex ripples.

9. The cell factory apparatus according to claim 1, characterized in that: The bottom surface of the cover plate is provided with scale lines, which are integrally formed with the cover plate.

10. The cell factory apparatus according to claim 1, characterized in that: The diameter of the injection hole is set at 11mm-12mm.

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