A protein chromatography system component

Abstract

This utility model discloses a protein chromatography system component. Relating to the field of protein chromatography technology, the component includes: a support column; a turntable rotatably connected to the upper end of the support column; rollers rotatably connected to the outer side of the turntable; four sets of rollers arranged circumferentially and rotatably connected to the outer side of the turntable; a stop block fixedly connected to the upper end of the support column directly above the turntable; a flexible tube fixedly connected between the stop block and the turntable; one end of the flexible tube extending outwards and having a liquid injection tube sleeved at its end; the liquid injection tube fixedly connected inside the chromatography column; a chromatography column sleeved at the bottom of the chromatography column; and a bottom outlet fixedly connected to the bottom of the chromatography column. Through the cooperation of the turntable, rollers, and stop block, the rollers squeeze the flexible tube to generate negative pressure, drawing in buffer solution. This allows for precise control of the buffer solution delivery rhythm, ensuring the stability of the gel column washing effect and helping to improve the sample separation effect within the column.

Description

Technical Field

[0001] This utility model relates to the field of protein chromatography technology, and in particular to a protein chromatography system component. Background Technology

[0002] In the field of protein research, protein chromatography is a crucial separation and analysis technique used to isolate and purify specific proteins from complex biological samples. Its principle is based on the physicochemical interactions between proteins and the chromatography medium, such as adsorption, ion exchange, and size exclusion. However, existing protein chromatography systems often face several problems that urgently need to be addressed in practical operation.

[0003] Traditional protein chromatography systems deliver buffer solutions manually, which requires continuous monitoring and operation by the operator. This increases labor costs to some extent, as the operator needs to manage multiple steps simultaneously, such as sample preparation, sample loading, and buffer delivery, increasing the difficulty and risk of errors.

[0004] Therefore, it is necessary to provide a protein chromatography system component to solve the above-mentioned technical problems. Utility Model Content

[0005] In view of the above situation and to overcome the defects of the existing technology, this utility model provides a protein chromatography system component that can automatically deliver buffer solution by using the cooperation of a turntable, rollers and baffles to generate negative pressure by squeezing the tubing with rollers.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] A protein chromatography system component includes: a support column, a turntable rotatably connected to the upper end of the support column, rollers rotatably connected to the outer side of the turntable, four sets of rollers arranged in a circumferential order and rotatably connected to the outer side of the turntable, a stop block fixedly connected to the upper end of the support column directly above the turntable, a flexible tube fixedly connected between the stop block and the turntable, one end of the flexible tube extending outward and the other end fitted with a liquid injection tube, the liquid injection tube fixedly connected inside a liquid injection layer, a chromatography column fitted at the bottom of the liquid injection layer, and a bottom outlet fixedly connected to the bottom of the chromatography column.

[0008] Preferably, a water outlet is fixedly connected to the bottom of the bottom outlet, and a valve is fixedly connected to the outside of the water outlet. The water outlet allows the buffer solution inside the chromatography column to flow out, and the valve allows the water outlet to be opened or closed as needed.

[0009] Preferably, a connecting rod is fixedly connected to the side of the support column, and a clamping platform is fixedly connected to the end of the connecting rod. The clamping platform has a clamping groove inside, and a telescopic rod slides through the outside of the clamping groove. A clamping pad is fixedly connected to one end of the telescopic rod near the inside of the clamping groove, and a handle is fixedly connected to the other end of the clamping groove. A spring is fixedly connected to the side of the clamping groove at the center of the telescopic rod, and the end of the spring is fixedly connected to the outside of the clamping pad. By pulling the handle, the telescopic rod is moved to the outside of the clamping platform. At this time, the clamping pad also moves with the telescopic rod to the inside of the clamping groove. The chromatography column can then be placed inside the clamping groove. After pulling the handle, the clamping pad will be pushed closer to the center of the clamping groove by the spring until the chromatography column is completely clamped.

[0010] Preferably, a buffer pad is provided on the other side of the clamping groove. The buffer pad and the clamping pad are made of rubber. When clamping the chromatography column, the rubber can play a buffering role, thereby protecting the chromatography column from damage. At the same time, the rubber material can prevent the chromatography column from rotating.

[0011] Preferably, a motor is fixedly connected to the back of the support column, and the motor drive end passes through the support column and is fixedly connected to the turntable. The motor can control the rotation of the turntable, thereby driving the buffer solution into the tubing and into the chromatography column through the injection tube.

[0012] Preferably, a hanging rod is fixedly connected to the middle position of the support column, and a support base plate is fixedly connected to the bottom of the support column. Excess hose pipe can be suspended on the top of the hanging rod to collect the hose pipe. The support base plate can provide support for the entire support column.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] (1) This utility model: By cooperating with the turntable, roller and stop, the roller squeezes the hose to generate negative pressure to draw in the buffer solution, thereby realizing the automatic delivery of the buffer solution. This design does not require complicated external pumping equipment, has a simple structure and low cost, and can accurately control the delivery rhythm of the buffer solution, ensuring the stability of the rinsing effect on the gel column, which helps to improve the separation effect of the sample in the column.

[0015] (2) This utility model: The clamping platform set on the side of the support column can move the telescopic rod and clamping pad by pulling the handle. With the elastic effect of the spring, the chromatography column can be easily clamped and fixed. The clamping pad and the buffer pad are made of rubber, which can not only play a buffering role and protect the chromatography column from damage during the clamping process, but also effectively prevent the chromatography column from rotating and ensure the stability of the chromatography process. Attached Figure Description

[0016] Figure 1A front view schematic diagram of a protein chromatography system component provided by this utility model;

[0017] Figure 2 for Figure 1 Enlarged schematic diagram of the structure at point A;

[0018] Figure 3 A frontal view structural schematic diagram of a protein chromatography system component provided by this utility model;

[0019] Figure 4 This is a schematic diagram of the rear view of a protein chromatography system component provided by this utility model.

[0020] The corresponding names of the reference numerals in the attached drawings are as follows: 1. Support column; 2. Connecting rod; 3. Clamping platform; 4. Clamping groove; 5. Buffer pad; 6. Clamping pad; 7. Telescopic rod; 8. Spring; 9. Handle; 10. Chromatography column; 11. Injection layer; 12. Injection tube; 13. Hose; 14. Turntable; 15. Stop block; 16. Roller; 17. Hanging rod; 18. Support base plate; 19. Bottom outlet; 20. Water outlet; 21. Valve; 22. Motor. Detailed Implementation

[0021] The present invention will be further described below with reference to the accompanying drawings and embodiments. The embodiments of the present invention include, but are not limited to, the following embodiments. Example

[0022] like Figure 1-4As shown, this utility model provides a protein chromatography system component, including: a support column 1, a turntable 14 rotatably connected to the upper end of the support column 1, rollers 16 rotatably connected to the outer side of the turntable 14, four sets of rollers 16 arranged circumferentially and rotatably connected to the outer side of the turntable 14, a stop block 15 fixedly connected to the upper end of the support column 1 directly above the turntable 14, a flexible tube 13 fixedly connected between the stop block 15 and the turntable 14, one end of the flexible tube 13 extending outward and the other end fitted with an injection tube 12, the injection tube 12 fixedly connected inside the chromatography column 11, a chromatography column 10 fitted at the bottom of the chromatography column 11, and a bottom outlet 19 fixedly connected to the bottom of the chromatography column 10. The swollen gel suspension is slowly poured into the chromatography column 10 while the bottom outlet 19 of the chromatography column 10 is opened. Allow the buffer solution to flow out, allowing the gel to settle naturally within the column. During the packing process, care should be taken to avoid air bubbles and layer breaks. A glass rod can be used to gently stir the gel suspension to help it settle evenly. Before adding the sample, connect the other end of the tubing 13 to the bottle containing the buffer solution. Rotate the turntable 14, causing the roller 16 to rotate. When the roller 16 reaches the top of the turntable 14, a stop 15 is fixedly connected to the top of the turntable 14, causing the roller 16 to compress the tubing 13, moving the gas inside the tubing forward. After the roller 16 is removed, the compressed portion of the tubing 13 quickly returns to its original shape, creating a negative pressure inside the tubing 13. Under this negative pressure, the buffer solution is drawn into the tubing 13. As the roller 16 continues to rotate, the buffer solution is delivered, allowing it to pass rapidly through the chromatography column 11. The sample enters the chromatography column 10, where the gel column is flushed to ensure that the buffer solution inside the gel column is consistent with the loading buffer, thus ensuring effective separation of the sample within the column. Then, the bottom outlet 19 is closed, and the prepared protein sample is slowly added to the top of the gel column using a syringe or micropipette. The bottom outlet 19 is then opened, allowing the protein sample to enter the gel column. When the protein sample is tangent to the gel surface, the rotation of the turntable 14 drives the eluent through the tubing 13 into the chromatography column 10. The eluent is then collected at the bottom of the outlet 19 at specific volumes or time intervals. The collected eluent can be used for subsequent analysis and identification. Example

[0023] The bottom outlet 19 is fixedly connected to a water outlet 20, and a valve 21 is fixedly connected to the outside of the water outlet 20. The water outlet 20 allows the buffer solution inside the chromatography column 10 to flow out. The valve 21 allows the water outlet 20 to be opened or closed as needed. Example

[0024] A connecting rod 2 is fixedly connected to the side of the support column 1. A clamping platform 3 is fixedly connected to the end of the connecting rod 2. A clamping groove 4 is provided inside the clamping platform 3. A telescopic rod 7 slides through the outside of the clamping groove 4. A clamping pad 6 is fixedly connected to one end of the telescopic rod 7 near the inside of the clamping groove 4. A handle 9 is fixedly connected to the other end of the clamping groove 4. A spring 8 is fixedly connected to the side of the clamping groove 4 at the center position of the telescopic rod 7. The end of the spring 8 is fixedly connected to the outside of the clamping pad 6. By pulling the handle 9, the telescopic rod 7 is moved to the outside of the clamping platform 3. At this time, the clamping pad 6 also moves to the inside of the clamping groove 4 along with the telescopic rod 7. At this time, the chromatography column 10 can be placed inside the clamping groove 4. Then, stop pulling the handle 9. At this time, under the action of the spring 8, the clamping pad 6 will be pushed closer to the center position of the clamping groove 4 until the chromatography column 10 is completely clamped. Example

[0025] A buffer pad 5 is provided on the other side of the clamping groove 4. The buffer pad 5 and the clamping pad 6 are made of rubber. When clamping the chromatography column 10, the rubber can play a buffering role, thereby protecting the chromatography column 10 from damage. At the same time, the rubber material can prevent the chromatography column 10 from rotating. Example

[0026] A motor 22 is fixedly connected to the back of the support column 1. The drive end of the motor 22 passes through the support column 1 and is fixedly connected to the turntable 14. The motor 22 can control the rotation of the turntable 14, thereby driving the buffer solution into the tubing 13 and into the chromatography column 10 through the injection tube 12. Example

[0027] A hanging rod 17 is fixedly connected to the middle position of the support column 1, and a support base plate 18 is fixedly connected to the bottom of the support column 1. The excess pipe of the hose 13 can be suspended on the top of the hanging rod 17 to collect the hose 13 pipe. The support base plate 18 can provide support for the entire support column 1.

[0028] During use, before adding the sample, connect the other end of the tubing 13 to the bottle containing the buffer solution. Start the motor 22, which drives the turntable 14 to rotate. The turntable 14 then drives the roller 16 to rotate. When the roller 16 reaches the top of the turntable 14, the presence of the stop 15 causes the roller 16 to squeeze the tubing 13, moving the gas inside the tubing 13 forward. After the roller 16 moves away, the compressed portion of the tubing 13 quickly returns to its original shape, creating a negative pressure inside the tubing 13. Under this negative pressure, the buffer solution is drawn into the tubing 13. As the roller 16 continues to rotate, the buffer solution quickly passes through the chromatography column 11 and enters the interior of the chromatography column 10, flushing the gel column inside the chromatography column 10. The buffer solution inside the gel column is made consistent with the loading buffer to ensure the separation effect of the sample in the column. After rinsing, the valve 21 corresponding to the bottom outlet 19 is closed. The prepared protein sample is slowly added to the top of the gel column using a syringe or micropipette. The valve 21 corresponding to the bottom outlet 19 is opened, and the protein sample enters the gel column. When the protein sample is tangent to the gel surface, the turntable 14 is rotated by the motor 22, which drives the eluent through the tubing 13 and the injection tube 12 into the chromatography column 10. The eluent is collected at the outlet 20 at the lower end of the bottom outlet 19. The eluent is collected at certain volumes or time intervals and is used for subsequent analysis and identification.

[0029] The above embodiments are merely one of the preferred embodiments of this utility model and should not be used to limit the scope of protection of this utility model. Any modifications or refinements made to the main design concept and spirit of this utility model that are not of substantial significance, but solve the same technical problem as this utility model, should be included within the scope of protection of this utility model.

Claims

1. A protein chromatography system component, characterized in that, include: A support column (1) is rotatably connected to the upper end of the support column (1). A roller (16) is rotatably connected to the outer side of the turntable (14). The roller (16) has four sets arranged in a circular order and rotatably connected to the outer side of the turntable (14). A stop block (15) is fixedly connected to the upper end of the support column (1) at the position directly above the turntable (14). A hose (13) is fixedly connected between the stop block (15) and the turntable (14). One end of the hose (13) extends outward and the end is fitted with an injection tube (12). The injection tube (12) is fixedly connected inside the injection layer (11). A chromatography column (10) is fitted at the bottom of the injection layer (11). A bottom outlet (19) is fixedly connected to the bottom of the chromatography column (10).

2. A protein chromatography system component according to claim 1, characterized in that, The bottom outlet (19) is fixedly connected to a water outlet (20), and a valve (21) is fixedly connected to the outside of the water outlet (20).

3. A protein chromatography system component according to claim 1, characterized in that, The support column (1) is fixedly connected to a connecting rod (2) on its side. The end of the connecting rod (2) is fixedly connected to a clamping platform (3). The clamping platform (3) is provided with a clamping groove (4) inside. A telescopic rod (7) slides through the outside of the clamping groove (4). A clamping pad (6) is fixedly connected to one end of the telescopic rod (7) near the inside of the clamping groove (4). A handle (9) is fixedly connected to the other end of the clamping groove (4). A spring (8) is fixedly connected to the side of the clamping groove (4) at the center of the telescopic rod (7). The end of the spring (8) is fixedly connected to the outside of the clamping pad (6).

4. A protein chromatography system component according to claim 3, characterized in that, A buffer pad (5) is provided on the other side of the clamping groove (4), and the buffer pad (5) and the clamping pad (6) are made of rubber.

5. A protein chromatography system component according to claim 1, characterized in that, A motor (22) is fixedly connected to the back of the support column (1), and the driving end of the motor (22) passes through the support column (1) and is fixedly connected to the turntable (14).

6. A protein chromatography system component according to claim 1, characterized in that, A hanging rod (17) is fixedly connected to the middle position of the support column (1), and a support base plate (18) is fixedly connected to the bottom of the support column (1).

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