A kit for simultaneous multiplex amplification of 74 STR loci

Abstract

This invention relates to a reagent kit for simultaneous multiplex amplification of 74 STR loci. The structure includes: a reagent kit body, a sealing cap, a first support block, and a second support block. The reagent kit body is a rectangular box structure with an open top. By installing the first and second support blocks and placing the reagent kit body close to a table, rotating either the first or second support block causes the first and second linkage components to slide in opposite directions, simultaneously causing the first and second support blocks to flip downwards. The first and second support blocks form a support structure, tilting the reagent kit body for easy access by testing personnel without requiring them to bend their necks to observe the interior, thus improving the convenience of the reagent kit body. Rotating the first or second support block again in the opposite direction resets the first and second support blocks and retracts them into the bottom of the reagent kit body, restoring the reagent kit body to a horizontal position.

Description

Technical Field

[0001] This invention relates to the field of reagent kit technology, and in particular to a reagent kit for simultaneous multiplex amplification of 74 STR loci. Background Technology

[0002] STR locus multiplex amplification is an important technique in forensic biological evidence. This technique is based on fluorescently labeled short tandem repeat loci and can simultaneously amplify multiple STR loci. Compared with single locus amplification, STR locus multiplex amplification has significant advantages. Its locus fragments are small and easy to amplify, making it particularly suitable for the detection of trace and degraded samples. It is also characterized by speed, efficiency, accuracy, sensitivity, and large information content. In practical applications, multiplex amplification can be used to simultaneously amplify multiple STR loci on a specific chromosome (such as the Y chromosome or autosomes), and then use a gene analyzer for fluorescence tracking detection to obtain rich genetic information, providing reliable evidence for forensic individual identification, paternity testing, and DNA genealogy construction.

[0003] The current design of the kit, capable of simultaneously amplifying 74 STR loci, has significant limitations. Due to its flat bottom, the internal components (such as reagent tubes and testing tools) are scattered, making it difficult for operators to quickly and accurately identify items because their view is obstructed by the kit's edges. This easily leads to mistakes, affecting the accuracy of test results and potentially causing the entire testing process to fail, resulting in wasted time and reagents. Furthermore, the horizontal design of the kit exacerbates the inconvenience, forcing operators to bend their necks for extended periods to see the internal components. This not only increases fatigue and reduces work efficiency but can also damage neck health, such as causing cervical pain. Therefore, the design of this kit urgently needs optimization in the placement and arrangement of components to improve the convenience and accuracy of the testing process.

[0004] Therefore, to address the above issues, a new kit for simultaneous multiplex amplification of 74 STR loci is proposed. Utility Model Content

[0005] To overcome the problems existing in related technologies, this utility model provides a multiplication amplification kit for 74 STR loci simultaneously. It can install a flip-up support structure at the bottom of the kit, which makes it easy to tilt the kit. The internal items will naturally slide towards the opening, and the testing personnel can quickly take them out without reaching deep or flipping the kit. It is especially suitable for high-frequency, small-batch retrieval scenarios. At the same time, the tilt angle can simulate a natural overhead view, eliminating the need for neck bending and alleviating fatigue caused by long-term operation.

[0006] To achieve the above objectives, the first aspect of this invention provides a kit for simultaneous multiplex amplification of 74 STR loci, comprising:

[0007] The reagent kit body, sealing cap, first support block, and second support block;

[0008] The reagent kit body is a rectangular box structure with an open top. A sealing cap is rotatably connected to the top of the reagent kit body, and a first support block is rotatably connected to the bottom of the reagent kit body. A second support block is provided on one side of the first support block and rotatably connected to the bottom of the reagent kit body. Both the first support block and the second support block are trapezoidal in shape.

[0009] Furthermore, both sides of the first support block and the second support block are fixedly connected with spur gears, and the inner wall of the reagent kit body is symmetrically rotatably connected with spur gears. The spur gears on both sides of the first support block are meshed with a first linkage component that is slidably connected to the inner wall of the reagent kit body, and the spur gears on both sides of the second support block are meshed with a second linkage component that is slidably connected to the inner wall of the reagent kit body. Both the first linkage component and the second linkage component are meshed with the spur gears on the inner wall of the reagent kit body.

[0010] Furthermore, the first linkage assembly consists of a first rack, a second rack, and a connecting rod;

[0011] The first support block has a first rack connected to the two sides by spur gears. The first rack has a second rack on one side that is connected to the spur gear on the inner wall of the reagent kit body. A connecting rod is fixedly connected to one side of the first rack. The side of the second rack near the first support block is fixedly connected to the connecting rod.

[0012] Furthermore, both the first support block and the second support block have rounded corners at their edges, and both the first support block and the second support block have symmetrically formed actuation grooves.

[0013] Furthermore, the top of the first support block and the second support block are symmetrically provided with first limiting holes, and the first support block and the second support block are symmetrically installed with limiting plugs that are slidably connected to the inner wall of the reagent kit body. The limiting plugs are slidably connected to the first limiting holes, and the limiting plugs are symmetrically fixedly connected with reset springs that are fixedly connected to the inner wall of the reagent kit body.

[0014] Furthermore, the surfaces of the first support block and the second support block near the limiting plug are symmetrically provided with second limiting holes, and the limiting plug slides through the second limiting holes.

[0015] Furthermore, a first magnetic strip is fixedly connected to the surface of the main body of the reagent kit, and a second magnetic strip is fixedly connected to the inner wall of the sealing cap. The first magnetic strip and the second magnetic strip are attracted to each other, and a silicone pad is fixedly connected to the adsorption surface of the second magnetic strip.

[0016] Furthermore, the surface of the silicone pad is wavy.

[0017] The technical solution provided by this utility model can include the following beneficial effects:

[0018] In this example, by installing the first and second support blocks, the reagent kit body is brought close to the table. Rotating the first or second support block causes the first and second linkage components to slide in opposite directions, causing the first and second support blocks to flip downwards simultaneously. The first and second support blocks form a support structure, tilting the reagent kit body, which makes it easier for testing personnel to quickly pick up the items without having to bend their necks to observe the inside, thus improving the convenience of the reagent kit body. Rotating the first or second support block in the opposite direction again resets the first and second support blocks and puts them into the bottom of the reagent kit body, restoring the reagent kit body to a horizontal position.

[0019] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit the present invention. Attached Figure Description

[0020] The above and other objects, features and advantages of the present invention will become more apparent from the accompanying drawings, in which like reference numerals generally represent like parts.

[0021] Figure 1 This is a schematic diagram of the overall structure shown in an embodiment of the present invention;

[0022] Figure 2 This is a schematic diagram of the main structure of the reagent kit at one angle, as shown in one embodiment of this utility model;

[0023] Figure 3 This is a schematic diagram of the main structure of the reagent kit from another angle, as shown in an embodiment of this utility model;

[0024] Figure 4 This is a schematic diagram showing the positions of the first linkage component and the second linkage component according to an embodiment of the present invention;

[0025] Figure 5 This is a schematic diagram of the structure of the first linkage component and the second linkage component shown in an embodiment of the present invention;

[0026] Figure 6 This is a schematic diagram showing the position of the limiting plug in an embodiment of the present invention;

[0027] Figure 7 This is a schematic diagram of the limiting plug structure shown in an embodiment of the present utility model;

[0028] Figure 8 This is a schematic diagram of the silicone pad structure shown in an embodiment of the present invention.

[0029] The correspondence between the labels and component names in the attached figures is as follows:

[0030] 1. Reagent kit body; 2. Sealing cap; 3. First support block; 4. Second support block;

[0031] 5. First linkage component; 6. Second linkage component; 7. Actuating groove;

[0032] 8. First limiting hole; 9. Limiting insert; 10. Return spring; 11. Second limiting hole;

[0033] 12. First magnetic strip; 13. Second magnetic strip; 14. Silicone pad. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model. The preferred embodiments of this utility model will now be described in more detail with reference to the accompanying drawings. Although the preferred embodiments of this utility model are shown in the drawings, it should be understood that this utility model can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to make this utility model more thorough and complete, and to fully convey the scope of this utility model to those skilled in the art.

[0035] The terminology used in this invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular forms “a,” “the,” and “the” used in this invention and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise. It should also be understood that the term “and / or” as used herein refers to and includes any or all possible combinations of one or more of the associated listed items.

[0036] It should be understood that although the terms "first," "second," "third," etc., may be used in this invention to describe various information, this information should not be limited to these terms. These terms are only used to distinguish information of the same type from one another. For example, without departing from the scope of this invention, first information may also be referred to as second information, and similarly, second information may also be referred to as first information. Thus, features defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0037] Designing a kit with a tilted structure that can amplify 74 STR loci is currently the primary technical problem that engineers need to solve.

[0038] To address the aforementioned issues, this utility model provides a reagent kit for simultaneous multiplex amplification of 74 STR loci. This structure allows for the installation of a flip-up support structure at the bottom of the kit, facilitating tilting the kit. The internal components naturally slide towards the opening, allowing operators to quickly retrieve the kit without reaching in or flipping it. This is particularly suitable for high-frequency, small-batch retrieval scenarios. Furthermore, the tilt angle simulates a natural overhead view, eliminating the need for neck bending and alleviating fatigue from prolonged operation.

[0039] The technical solution of the present invention (Embodiment 1) is described in detail below with reference to the accompanying drawings.

[0040] Figure 1 This is a schematic diagram of the overall structure shown in an embodiment of the present invention; Figure 2 This is a schematic diagram of the main structure of the reagent kit at one angle, as shown in one embodiment of this utility model; Figure 3 This is a schematic diagram of the main structure of the reagent kit from another angle, as shown in an embodiment of this utility model; Figure 4 This is a schematic diagram showing the positions of the first linkage component and the second linkage component according to an embodiment of the present invention; Figure 5 This is a schematic diagram of the structure of the first linkage component and the second linkage component shown in an embodiment of the present invention; Figure 6 This is a schematic diagram showing the position of the limiting plug in an embodiment of the present invention; Figure 7 This is a schematic diagram of the limiting plug structure shown in an embodiment of the present utility model; Figure 8 This is a schematic diagram of the silicone pad structure shown in an embodiment of the present invention.

[0041] See Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8 This kit simultaneously performs multiplex amplification on 74 STR loci, specifically including:

[0042] The reagent kit consists of: 1. main body; 2. sealing cap; 3. first support block; and 4. second support block.

[0043] The reagent kit body 1 is a rectangular box structure with an open top. A sealing cap 2 is rotatably connected to the top of the reagent kit body 1. A first support block 3 is rotatably connected to the bottom of the reagent kit body 1. A second support block 4 is provided on one side of the first support block 3 and is rotatably connected to the bottom of the reagent kit body 1. Both the first support block 3 and the second support block 4 are trapezoidal in shape.

[0044] Specifically, both sides of the first support block 3 and the second support block 4 are fixedly connected with spur gears, and the inner wall of the reagent kit body 1 is symmetrically rotatably connected with spur gears. The spur gears on both sides of the first support block 3 are meshed with a first linkage component 5 that is slidably connected to the inner wall of the reagent kit body 1. The spur gears on both sides of the second support block 4 are meshed with a second linkage component 6 that is slidably connected to the inner wall of the reagent kit body 1. Both the first linkage component 5 and the second linkage component 6 are meshed with the spur gears on the inner wall of the reagent kit body 1.

[0045] Specifically, the first linkage assembly 5 consists of a first rack, a second rack, and a connecting rod;

[0046] The first support block 3 has a first rack connected to the two sides by a spur gear. The first rack has a second rack on one side that is connected to the spur gear on the inner wall of the reagent kit body 1. A connecting rod is fixedly connected to one side of the first rack. The side of the second rack near the first support block 3 is fixedly connected to the connecting rod.

[0047] Specifically, the edges of the first support block 3 and the second support block 4 are rounded, and the first support block 3 and the second support block 4 are symmetrically provided with actuation grooves 7.

[0048] Specifically, the first support block 3 and the second support block 4 are symmetrically provided with first limiting holes 8 at their top ends. The first support block 3 and the second support block 4 are symmetrically installed with limiting plugs 9 that are slidably connected to the inner wall of the reagent kit body 1. The limiting plugs 9 are slidably connected to the first limiting holes 8. The limiting plugs 9 are symmetrically fixedly connected with reset springs 10 that are fixedly connected to the inner wall of the reagent kit body 1.

[0049] Specifically, the first support block 3 and the second support block 4 are symmetrically provided with second limiting holes 11 on the surfaces near the limiting plug 9, and the limiting plug 9 slides through the second limiting holes 11.

[0050] Specifically, a first magnetic strip 12 is fixedly connected to the surface of the main body 1 of the reagent kit, and a second magnetic strip 13 is fixedly connected to the inner wall of the sealing cap 2. The first magnetic strip 12 and the second magnetic strip 13 are attracted to each other, and a silicone pad 14 is fixedly connected to the adsorption surface of the second magnetic strip 13.

[0051] Specifically, the surface of the silicone pad 14 is wavy.

[0052] In this embodiment, how to switch the main body 1 of the reagent kit between a tilted state and a horizontal state is described, in conjunction with... Figures 1 to 5 The specific implementation method is as follows: Place the reagent kit body 1 close to the table, insert your finger into the actuating groove 7, and drive the first support block 3 or the second support block 4 to flip, causing the first linkage component 5 and the second linkage component 6 to slide in opposite directions, causing the first support block 3 and the second support block 4 to flip downwards at the same time. The first support block 3 and the second support block 4 form a support structure, tilting the reagent kit body 1, which makes it convenient for the testing personnel to quickly pick up the items without having to bend their necks to observe the inside, thus improving the convenience of the reagent kit body 1. Rotate the first support block 3 or the second support block 4 in the opposite direction again, reset the first support block 3 and the second support block 4 and put them into the bottom of the reagent kit body 1, and the reagent kit body 1 returns to the horizontal placement state.

[0053] In this embodiment, how to limit the first support block 3 and the second support block 4, combined with Figure 6 and Figure 7 The specific implementation method is as follows: the first support block 3 and the second support block 4 are rotated into the bottom end of the reagent kit body 1, the reset spring 10 squeezes the limiting plug 9 into the first limiting hole 8 to limit the first support block 3 and the second support block 4 horizontally, ensuring that the reagent kit body 1 is in a horizontal position. The first support block 3 and the second support block 4 are flipped downwards, and the limiting plug 9 enters the second limiting hole 11 to limit the first support block 3 and the second support block 4 vertically, ensuring that the reagent kit body 1 is in an inclined setting state.

[0054] In this embodiment, how to improve the adsorption effect of the reagent kit body 1 and the sealing cap 2, combined with Figure 8 The specific implementation method is as follows: when the reagent kit body 1 is closed, the sealing cap 2 is close to the reagent kit body 1, the second magnetic strip 13 is close to the first magnetic strip 12, the compressed silicone pad 14 fills the gap, compensates for the uneven parts of the surfaces of the first magnetic strip 12 and the second magnetic strip 13, increases the actual contact area, improves the adsorption effect, and can also buffer vibration and reduce the risk of the second magnetic strip 13 falling off due to impact.

[0055] The present invention has been described in detail above with reference to the accompanying drawings. In the above embodiments, the descriptions of each embodiment have different focuses; for parts not described in detail in a certain embodiment, please refer to the relevant descriptions of other embodiments. Those skilled in the art should also understand that the actions and modules involved in the specification are not necessarily essential to the present invention. Furthermore, it is understood that the steps in the method of the present invention embodiments can be adjusted, combined, and deleted according to actual needs, and the structure in the device of the present invention embodiments can be combined, divided, and deleted according to actual needs.

[0056] The various embodiments of the present invention have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A kit for simultaneous multiplex amplification of 74 STR loci, characterized in that, include: The reagent kit body (1), sealing cap (2), first support block (3), and second support block (4); The reagent kit body (1) is a rectangular box structure with an open top. A sealing cap (2) is rotatably connected to the top of the reagent kit body (1). A first support block (3) is rotatably connected to the bottom of the reagent kit body (1). A second support block (4) is provided on one side of the first support block (3) and rotatably connected to the bottom of the reagent kit body (1). Both the first support block (3) and the second support block (4) are trapezoidal in shape.

2. The kit for simultaneous multiplex amplification of 74 STR loci according to claim 1, characterized in that: Both sides of the first support block (3) and the second support block (4) are fixedly connected with flat gears. The inner wall of the reagent kit body (1) is symmetrically connected with flat gears. The flat gears on both sides of the first support block (3) are meshed with a first linkage component (5) that is slidably connected to the inner wall of the reagent kit body (1). The flat gears on both sides of the second support block (4) are meshed with a second linkage component (6) that is slidably connected to the inner wall of the reagent kit body (1). Both the first linkage component (5) and the second linkage component (6) are meshed with the flat gears on the inner wall of the reagent kit body (1).

3. The kit for simultaneous multiplex amplification of 74 STR loci according to claim 2, characterized in that: The first linkage assembly (5) consists of a first rack, a second rack, and a connecting rod; The first support block (3) has a first rack connected to the two sides by a flat gear meshing. The first rack has a second rack on one side that meshes with the flat gear on the inner wall of the reagent kit body (1). A connecting rod is fixedly connected to one side of the first rack. The side of the second rack near the first support block (3) is fixedly connected to the connecting rod.

4. The kit for simultaneous multiplex amplification of 74 STR loci according to claim 2, characterized in that: The edges of the first support block (3) and the second support block (4) are rounded, and the first support block (3) and the second support block (4) are symmetrically provided with actuation grooves (7).

5. The kit for simultaneous multiplex amplification of 74 STR loci according to claim 2, characterized in that: The first support block (3) and the second support block (4) are symmetrically provided with first limiting holes (8) at their top ends. The first support block (3) and the second support block (4) are symmetrically provided with limiting plugs (9) that are slidably connected to the inner wall of the reagent kit body (1). The limiting plugs (9) are slidably connected to the first limiting holes (8). The limiting plugs (9) are symmetrically fixedly connected with reset springs (10) that are fixedly connected to the inner wall of the reagent kit body (1).

6. The kit for simultaneous multiplex amplification of 74 STR loci according to claim 5, characterized in that: The first support block (3) and the second support block (4) are symmetrically provided with second limiting holes (11) on the surface near the limiting plug (9), and the limiting plug (9) slides through the second limiting hole (11).

7. The kit for simultaneous multiplex amplification of 74 STR loci according to claim 1, characterized in that: The main body (1) of the reagent kit is fixedly connected to a first magnetic strip (12), and the inner wall of the sealing cap (2) is fixedly connected to a second magnetic strip (13). The first magnetic strip (12) and the second magnetic strip (13) are attracted to each other, and a silicone pad (14) is fixedly connected to the adsorption surface of the second magnetic strip (13).

8. The kit for simultaneous multiplex amplification of 74 STR loci according to claim 7, characterized in that: The surface of the silicone pad (14) is wavy.

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