An automated in vitro biosynthesis reaction system

Abstract

The utility model discloses an automatic in-vitro biosynthesis reaction system, comprising: reaction tank subassembly, mix and move subassembly, feed assembly, reaction raw material quantity feedback unit, detection unit and with preceding each part communication connection's control device, reaction tank subassembly has the tank body for accommodating reaction liquid and carries out in-vitro biological reaction, reaction mix and move subassembly is used for realizing the mix and move of reaction liquid, feed assembly has with the feed pipe of tank body intercommunication, with the feed pump intercommunication of feed pipe, reaction raw material quantity feedback unit is used for detecting the raw material quantity in tank body to get raw material quantity parameter, detection unit is used for detecting reaction liquid and / or the gas generated in reaction to get detection parameter, control device has feed module, operation module and mix and move module, is used for realizing the automatic feed, automatic stirring, automatic detection, automatic discharge, automatic cleaning etc.

Description

Technical Field

[0001] This invention relates to the field of in vitro biosynthesis, and in particular to an automated in vitro biosynthesis reaction system. Background Technology

[0002] In vitro biological reactions, such as cell-free in vitro biosynthesis, involve obtaining the essential components required for transcription and translation in cells, adding DNA templates in vitro to maintain gene transcription, protein translation, or metabolic processes, thereby synthesizing the target product. Cell-free synthetic biology (CFSE) removes the cell membrane, allowing direct regulation of intracellular life activities; it removes the natural genome, eliminating non-essential gene regulation and decoupling cell growth from core metabolic regulation; the system is open, with no obstacles to material transport, facilitating the addition of substrates, removal of products, and monitoring and analysis of the process, offering the greatest degree of freedom in engineering. It plays a crucial role in both basic sciences and engineering applications: elucidating life systems such as protein translation mechanisms; and it has broad application potential in structural biology, high-throughput screening, biocatalysis, and biomedicine.

[0003] While existing cell-free in vitro biosynthesis systems offer simpler separation and purification of target products and yield relatively homogeneous products, they all rely on laboratory-scale or U-tube extraction methods, resulting in low yields. To rapidly synthesize large quantities of target products in a short time, the reaction efficiency of the system needs to be significantly improved. Therefore, a novel automated in vitro bioreactor system is urgently needed to address these issues.

[0004] Currently, most in vitro biosynthesis processes utilize open stirred tanks. These rely on ventilation ducts to blow hot and cold air to control the temperature of the reaction solution, disperse gases generated during the reaction to reduce their interference with the reaction, and manually test small samples to control the quality of the reaction solution. This approach is inconvenient, time-consuming, and labor-intensive. Real-time control of the reaction solution quality is impossible, requiring operators to constantly monitor the reaction solution and take appropriate actions. Furthermore, the target products in in vitro biosynthesis are diverse, requiring operators to adjust intervention conditions according to the specific product. The inherent limitations imposed by human intervention easily lead to instability in the overall quality control of the reaction solution. The open, manual operation process also carries the risk of contact exposure and increases the possibility of reaction solution contamination, causing unnecessary negative impacts. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides an automated in vitro biosynthesis reaction system, comprising: a reaction vessel assembly, a mixing assembly, a feeding assembly, a reaction raw material quantity feedback unit, a detection unit, and a control device communicatively connected to the aforementioned components. The reaction vessel assembly has a vessel for containing the reaction liquid for in vitro biosynthesis; the reaction mixing assembly is used to mix the reaction liquid; the feeding assembly has a feed pipe connected to the vessel and a feed pump connected to the feed pipe; the reaction raw material quantity feedback unit is used to detect the amount of raw material in the vessel to obtain raw material quantity parameters; the detection unit is used to monitor the reaction liquid and / or... The gas produced by the reaction is detected to obtain detection parameters; the control device has a feeding module, an operation module, and a mixing module; wherein, the feeding module controls the feeding pump to pump the reaction raw materials into the tank through the feeding pipe; the operation module obtains the raw material quantity parameters and at least determines whether the start-up conditions are met based on the raw material quantity parameters, if so, it generates a start-up signal and sends it to the mixing module; after receiving the start-up signal, the mixing module starts the mixing component to perform mixing for a predetermined time and a predetermined speed; the operation module is also communicatively connected to the detection unit to receive the detection parameters and control the reaction process according to the detection parameters.

[0006] Specifically, the inner wall of the tank is uniformly provided with a plurality of protruding structures; and / or the tank is provided with a plurality of stirring blades, which may or may not be rotatable; the non-rotatable stirring blades are arranged at intervals along the inner peripheral wall of the tank, and preferably, the stirring blades are provided with a plurality of flow holes, and the spacing between each adjacent flow hole is equal.

[0007] Specifically, the hybrid assembly includes a large gear, a small gear, and a drive device. The large gear is radially fixed around the outside of the reaction vessel via a gear connecting plate. The small gear meshes with the large gear and is mounted on the rotating shaft of the drive device. The drive device completes the hybridization by driving the small gear to rotate.

[0008] Specifically, the reaction material quantity feedback unit has a weighing sensor installed at the bottom of the tank, and the weighing sensor is communicatively connected to the operating module.

[0009] Specifically, the detection unit includes: a reaction condition detection unit and / or a reaction liquid parameter detection unit; the reaction condition detection unit includes: a reaction liquid temperature detection component that obtains a reaction liquid temperature parameter by detecting the reaction liquid temperature and / or a gas detection component that obtains an ethanol gas concentration parameter by detecting the concentration of ethanol gas generated in the reaction within the tank; the reaction liquid parameter detection unit includes: a liquid detection component that obtains a reaction liquid physicochemical parameter by detecting the reaction liquid physicochemical parameter, and / or an online quantitative detection device for reaction products that obtains a reaction product content parameter by detecting the content of reaction products generated in the reaction liquid; preferably, the operation module further determines whether the start-up conditions are met based on the reaction liquid temperature parameter and the ethanol gas concentration parameter.

[0010] Specifically, the operating module also determines the activity of the reaction solution based on its physicochemical parameters:

[0011] When the physicochemical parameters of the reaction solution do not exceed the preset activity range, the reaction solution is effective and the reaction continues; when the physicochemical parameters of the reaction solution exceed the preset activity range, the reaction solution is ineffective.

[0012] Furthermore, if the duration of the reaction solution's activity failure does not exceed the preset failure time range, the reaction does not fail and continues; if it exceeds the preset failure time range, the reaction fails.

[0013] Specifically, the operating module also adjusts the operating conditions based on the reaction liquid temperature parameter and / or the ethanol gas concentration parameter:

[0014] When the temperature parameter of the reaction solution and / or the concentration parameter of the ethanol gas do not meet the preset temperature range and / or preset ethanol concentration range, the operation module activates and regulates the corresponding temperature control component and gas regulation component.

[0015] Furthermore, if the duration of the non-compliance does not exceed the corresponding preset temperature non-compliance automatic recovery time range and / or preset ethanol concentration non-compliance automatic recovery time range, the reaction continues; if the duration of the non-compliance exceeds the corresponding preset temperature automatic recovery time range and / or preset gas concentration automatic recovery time range, the reaction stops.

[0016] Furthermore, if the duration of the non-compliance does not exceed the corresponding preset temperature non-compliance stop time range and / or preset ethanol concentration non-compliance stop time range, the reaction will automatically recover and continue; if the duration of the non-compliance exceeds the corresponding preset temperature non-compliance stop time range and / or preset ethanol concentration non-compliance stop time range, it is necessary to determine whether the physicochemical parameters of the reaction solution meet the preset activity range. If they do, the reaction continues; if they do not, the reaction fails.

[0017] Specifically, the reaction liquid temperature detection component includes a reaction liquid temperature sensor installed on the feed pipe 8 near the tank. The reaction liquid temperature sensor sends the reaction liquid temperature parameters to the operating module in real time to determine whether to perform temperature control adjustment.

[0018] Specifically, the gas detection assembly includes: a main gas exchange pipe, a suction pipe, and an ethanol gas concentration detection device; the first end of the main gas exchange pipe extends into the reaction vessel through a sealed bearing, and the second end of the main gas exchange pipe is located outside the reaction vessel; a portion of the suction pipe is nested inside the main gas exchange pipe, with one end extending out of the reaction vessel from the second end of the main gas exchange pipe and connected to the ethanol gas concentration detection device, and the other end protruding from the first end of the main gas exchange pipe and located inside the reaction vessel; preferably, the suction pipe extends upwards out of the first end; the ethanol gas concentration detection device extracts gas from the vessel through the suction pipe and inputs it into the ethanol gas concentration detection device to detect the ethanol gas concentration in the extracted gas; the ethanol sensor in the ethanol gas concentration detection device sends the ethanol gas concentration parameter to the operating module to determine whether to adjust the ethanol gas concentration.

[0019] Specifically, the ethanol gas concentration detection device includes a suction component, a first suction tube, a transfer detection chamber, and a second suction tube connected in sequence; wherein, the transfer detection chamber includes a detection cavity and an ethanol sensor, the gas drawn out by the suction tube is introduced from the second suction tube, and the gas detected by the detection cavity is discharged through the discharge port, preferably through the suction component.

[0020] Specifically, the liquid detection assembly includes: a sealed detection housing, multiple physicochemical sensors, and a liquid detection pipeline; the detection housing is used to contain the reaction liquid from the tank as a detection sample, and the probes of the sensors are inserted to detect the sample; the liquid detection pipeline connects the detection housing and the tank to form a circulating liquid detection path, and the detection sample enters the detection housing from the liquid detection pipeline and then flows back to the tank; the physicochemical sensors send the physicochemical parameters of the reaction liquid to the operating module to determine whether to pause, continue, or end the mixing.

[0021] Specifically, the sensor is a dissolved oxygen sensor, pH sensor, turbidity sensor, conductivity sensor, reaction solution concentration sensor, reaction solution temperature sensor, reaction solution flow sensor, or liquid level sensor.

[0022] Specifically, the online quantitative detection device for reaction products includes: a detection chamber, an optical device, a detection unit, a sample flow pipeline, and a sample detection tube; the detection chamber has a detection port; the optical device includes a light source located in the detection chamber, which is used to excite the reaction liquid from the container to generate light to be detected; the sample detection tube is used to contain the reaction liquid, at least a portion of which is placed in the detection chamber and is a transparent portion, where the reaction liquid is excited by the light source to generate the light to be detected; the detection unit collects the light to be detected through the detection port and converts it into an electrical signal; the electrical signal transmits the reaction product content parameter to the operating module to determine whether to pause, continue, or terminate the mixing; the sample flow pipeline has an inlet section and an outlet section respectively connected to the sample detection tube, whereby the reaction liquid enters the sample detection tube from the inlet section and flows through the transparent portion, and then exits from the outlet section.

[0023] Specifically, it also includes a gas sterilization unit, which includes an ultraviolet lamp installed outside the reaction vessel assembly and inside a housing surrounding the reaction vessel assembly. The ultraviolet lamp is controlled by the operation module and remains on during the hybridization process.

[0024] Specifically, the tank is equipped with an inspection door, which is fitted with a magnetic door sensor. The magnetic door sensor sends a magnetic door signal to the operating module, which then determines whether the start-up conditions are met based on the magnetic door signal.

[0025] Specifically, it also includes a discharge assembly, which has a discharge pipe connected to the tank body and a discharge pump connected to the discharge pipe.

[0026] Specifically, a portion of the discharge pipe extends into the tank body through a slip ring. One end of the portion extending into the tank body extends radially towards the circumferential edge along the inner wall of the tank body, while the other end is connected to the rotor interface of the slip ring.

[0027] Specifically, it also includes a circulating temperature control component, which includes: a coil sleeved on the outer wall of the tank and tightly fitted therewith, a constant temperature medium tank, and a circulating temperature control pipeline connecting the coil and the constant temperature medium tank to form a circulating passage for the medium.

[0028] Specifically, the operating module also includes a temperature control module, which adjusts the set temperature of the constant temperature medium tank according to the obtained reaction liquid temperature parameters to achieve temperature control.

[0029] Specifically, the coil is formed by folding a metal tube in half and then spirally coiling it with equal diameter. The coil has two parallel ports arranged adjacent to each other, which are the coil inlet and the coil outlet, respectively.

[0030] Specifically, the coil inlet and the coil outlet are connected to the medium outlet and medium inlet in the circulating temperature control pipeline respectively via slip rings.

[0031] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0032] The in vitro bioreactor system can automate the entire process of feeding, stirring, unloading, and cleaning, reducing the workload and operational knowledge requirements of operators; at the same time, it can control the reaction process based on real-time detection parameters, achieving full-process quality control in a time-saving and labor-saving manner.

[0033] 2. The main ventilation pipe facilitates air exchange between the inside and outside of the tank during the operation of the reaction system. At the same time, the extraction pipe can accelerate the discharge of gas from the tank through the suction components, thereby minimizing the concentration of ethanol gas below the safe threshold.

[0034] 3. The circulating temperature control component maintains constant temperature control of the tank during the operation of the reaction system, ensuring that the reaction liquid remains within the set temperature threshold range.

[0035] 4. The testing department conducts online testing during the operation of the reaction system, monitoring the reaction liquid temperature, ethanol gas concentration, physicochemical parameters of the reaction liquid, and the content of reaction products in real time, thereby achieving full-process quality control.

[0036] 5. The system can automatically manage various parameters in the production process, enabling one-click production and further simplifying operations. At the same time, operators can conduct trial production first, then save the parameters and perform one-click production, realizing the free switching between product sample trial production and mass production. Attached Figure Description

[0037] To more clearly illustrate the technical solution of this utility model, the drawings used in this utility model will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0038] Figure 1 This is an exploded view of the in vitro biosynthesis reaction system.

[0039] Figure 2 This is an assembly and disassembly diagram of the reaction vessel components and some circulating temperature control components.

[0040] Figure 3This is a schematic diagram of the coil.

[0041] Figure 4 This is a schematic diagram showing the assembly and disassembly of the circulating temperature control component.

[0042] Figure 5 This is another schematic diagram of the circulating temperature control component.

[0043] Figure 6 This is a cross-sectional view showing the connection between the liquid detection component and the reaction vessel component.

[0044] Figure 7 This is a cross-sectional view of the main ventilation pipe.

[0045] Figure 8 This is an exploded view of the ethanol gas concentration detection device assembly.

[0046] Figure 9 This is a schematic diagram of an online quantitative detection device for reaction products.

[0047] Figure 10 yes Figure 9 A cross-sectional view of the light source illumination channel in the image.

[0048] The reference numerals in the figures are as follows: 1. Outer shell assembly, 2. Structural support frame, 3. Reactor assembly, 31. Tank body, 32. Through-view monitoring door, 33. End cap, 34. Roller track, 35. Stirring blade, 36. Tail sealing plate, 361. Slip ring connector, 37. Gear connecting plate, 38. Large gear, 39. Slip ring, 4. Gas detection assembly, 41. Main ventilation pipe, 42. Sealed bearing, 43. Main ventilation pipe fixing seat, 44. Suction pipe, 45. Feed connection sleeve, 46. First suction pipe, 47. Transfer detection chamber, 471. Ethanol sensor, 472. Sealing gasket, 48. Second suction pipe, 49. Suction component, 5. Liquid 51. Detection box, 52. Sensor, 53. Float switch, 54. Liquid detection pipeline, 6. Circulation temperature control assembly, 61. Coil, 62. Coil retaining strip, 63. Circulation temperature control pipeline, 64. Constant temperature medium tank, 65. Sponge, 66. Medium circulation pump, 7. Online quantitative detection equipment for reaction products, 71. Detection device, 72. Optical device, 73. Optical path setting, 74. Detection chamber, 75. Light source, 76. Sample flow pipeline, 77. Inlet and outlet sections, 78. Sample detection tube, 8. Feed tube. Detailed Implementation

[0049] The technical solutions of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0050] This invention provides an automated in vitro biosynthesis reaction system, comprising: a reaction vessel assembly 3, a mixing assembly, a feeding assembly, a reaction raw material quantity feedback unit, a detection unit, and a control device communicatively connected to the aforementioned components. The reaction vessel assembly 3 has a vessel body 31 for containing the reaction liquid for in vitro biosynthesis; the reaction mixing assembly is used to mix the reaction liquid; the feeding assembly has a feed pipe 8 connected to the vessel body 31 and a feed pump connected to the feed pipe 8; the reaction raw material quantity feedback unit is used to detect the amount of raw material in the vessel body 31 to obtain raw material quantity parameters; the detection unit is used for... The reaction liquid and / or the gas produced by the reaction are detected to obtain detection parameters. The control device has a feeding module, an operation module, and a mixing module. The feeding module controls the feeding pump to pump the reaction raw materials into the tank through the feeding pipe 8. The operation module obtains the raw material quantity parameters and at least determines whether the start-up conditions are met based on the raw material quantity parameters. If so, it generates a start-up signal and sends it to the mixing module. After receiving the start-up signal, the mixing module starts the mixing component to perform mixing for a predetermined time and speed. The operation module is also connected to the detection unit to receive detection parameters and control the reaction process according to the detection parameters. In this embodiment, the mixing refers to the mixing effect achieved by controlling the mixing component through the mixing module to drive the tank 31 to rotate or to drive the rotatable stirring blade 35 inside the tank 31 to rotate. The fully automatic in vitro biosynthesis reaction system of this application can realize the full automation of the feeding, stirring, unloading, and cleaning process, reducing the workload and operational knowledge requirements of the operators. At the same time, it can also control the reaction process according to real-time detection parameters, achieving full-process quality control in a time-saving and labor-saving manner.

[0051] In one embodiment, a plurality of protruding structures are evenly arranged on the inner wall of the tank 31; and / or a plurality of stirring blades 35 are arranged inside the tank 31, the stirring blades 35 being rotatable or non-rotatable; the non-rotatable stirring blades 35 are spaced apart along the inner peripheral wall of the tank 31, preferably, the stirring blades 35 are provided with a plurality of flow holes, the spacing between adjacent flow holes being equal. Specifically, the main body of the tank 31 is made of stainless steel, and is provided with 3-6 stirring blades 35, the stirring blades 35 being integrally formed from a single piece with a smooth surface, and fixed to the tank wall by welding; polishing at the weld seam can improve the surface smoothness, reduce the obstruction to the flow of the reaction liquid, accelerate the mixing of the reaction liquid, and improve the reaction efficiency. Figure 6As shown, multiple flow holes are arranged on the contour surface of each stirring blade 35. This allows the reaction liquids within the areas divided by the stirring blades 35 to interact through these flow holes, improving the mixing effect. For better mixing efficiency, the loading height of the reaction liquid should not be too high; generally, the height of the stirring blades 35 should be 1-100 mm higher than the reaction liquid.

[0052] In one embodiment, the hybrid assembly includes: a large gear 38, a small gear, and a drive unit. The large gear 38 is radially fixed to the outside of the reactor body 31 via a gear connecting plate 37. The small gear meshes with the large gear 38 and is mounted on the rotating shaft of the drive unit. The drive unit completes the hybridization by driving the small gear to rotate. Specifically, as shown... Figure 1 , 2 As shown, the tank body 31 has a sealing cover assembly at both the front and rear. The rear sealing cover assembly is penetrated by a slip ring 39 and includes a tail sealing plate 36 that seals the rear end of the tank body 31 and a gear connecting plate 37 that connects and fixes a large gear 38 to the tail sealing plate 36. A slip ring connector 361 is also provided in the middle of the tail sealing plate 36 to connect the slip ring 39. The large gear 38 is located at the very end of the rear sealing cover assembly.

[0053] In one embodiment, the large gear 38 and the small gear are helical gears; preferably, the helical gears are 15° helical gears. The large gear 38 and the small gear, as meshing helical gears, have opposite directions of rotation, i.e., one is a left-hand helical gear and the other is a right-hand helical gear. The helical gears are 15° helical gears. The tooth line of a helical gear winds along a cylinder, making it a type of cylindrical gear. Classified by gear shaft, helical gears belong to parallel shaft gears. Helical gear transmissions have advantages such as good meshing performance, high overlap ratio, and high transmission accuracy because helical gears can better eliminate the influence of tooth pitch error on accuracy. The principle is that the torque fluctuation caused by friction is smaller. Spur gears mesh with a straight-line contact, while helical gears mesh with a curve-curve contact; therefore, the principle of friction activation is different. Compared to spur gears, helical gear transmissions have higher accuracy and also have advantages such as high strength or low noise / vibration, ensuring smoother operation of the transmission system.

[0054] In one embodiment, the raw material quantity feedback unit has a weighing sensor located at the bottom of the tank 31, and the weighing sensor is communicatively connected to the operating module. Specifically, during feeding, the operating module reads the current equipment weight from the weighing sensor and compares it with the weight before feeding to determine whether the current raw material quantity parameter has reached the preset feeding amount; similarly, during discharging, the operating module reads the current equipment weight from the weighing sensor and compares it with the weight before discharging to determine whether the current raw material quantity parameter has reached the preset discharging amount.

[0055] In one embodiment, the detection unit includes: a reaction condition detection unit and / or a reaction liquid parameter detection unit. The reaction condition detection unit includes: a reaction liquid temperature detection component that obtains a reaction liquid temperature parameter by detecting the temperature of the reaction liquid and / or a gas detection component 4 that obtains an ethanol gas concentration parameter by detecting the concentration of ethanol gas generated in the tank during the reaction; the reaction liquid parameter detection unit includes: a liquid detection component 5 that obtains physicochemical parameters of the reaction liquid by detecting the physicochemical parameters of the reaction liquid, and / or an online quantitative detection device 7 for reaction products that obtains a reaction product content parameter by detecting the content of reaction products generated in the reaction liquid.

[0056] In another embodiment, the operating module also determines whether the start-up conditions are met based on the reaction solution temperature parameter and / or ethanol gas concentration parameter. Taking in vitro protein synthesis as an example, the bioreactor used for in vitro biosynthesis is extremely sensitive to temperature. Protein raw materials are typically stored at -18 degrees Celsius, and after mixing into a reaction solution, low-temperature storage is also required. Heating is used to promote the reaction, but excessively high temperatures can lead to protein inactivation, while excessively low temperatures can affect reaction efficiency, both impacting the activity of the reaction solution. Current manual temperature monitoring of the reaction solution is limited by human factors, and the temperature detection results cannot be updated in real time, which is detrimental to improving reaction efficiency and controlling reaction quality. The bioreactor releases ethanol gas during the reaction process. Excessively high ethanol gas concentrations can inhibit protein reactions; therefore, stirring the reaction solution is necessary during production to ensure sufficient reaction and ethanol gas release. In summary, ensuring that the ethanol gas concentration in the reaction solution is below a safe threshold and that the temperature of the reaction solution does not exceed a preset temperature threshold range are crucial for improving the quality of most bioreactor stirring reactions. Furthermore, the operating module of this application also determines whether the start-up conditions are met based on the reaction liquid temperature parameters and / or ethanol gas concentration parameters, which is beneficial for strictly controlling the reaction quality at the initial stage and improving reaction efficiency.

[0057] Specifically, in one embodiment, the determination of the start-up conditions includes: whether the raw material quantity parameter reaches the preset feed quantity, whether the reaction liquid temperature parameter is within the preset temperature threshold range, and whether the ethanol gas concentration parameter exceeds the preset upper limit of the ethanol gas concentration threshold. The above conditions must be met simultaneously to generate a start-up signal and send it to the hybrid module.

[0058] In one embodiment, the fully automated in vitro biosynthesis reaction system of this application can also automatically manage various parameters in the production process, enabling one-click production and further simplifying the operation. At the same time, the operator can first conduct trial production, then save the parameters, and then conduct one-click production, realizing the free switching from product sample trial production to mass production.

[0059] In one embodiment, the operating module also determines the activity of the reaction solution based on its physicochemical parameters: when the physicochemical parameters of the reaction solution do not exceed the preset activity range, the reaction solution is active and the reaction continues; when the physicochemical parameters of the reaction solution exceed the preset activity range, the reaction solution is inactive; furthermore, when the duration of the inactive reaction solution does not exceed the preset inactive time range, the reaction does not fail and the reaction continues; if it exceeds the preset inactive time range, the reaction fails.

[0060] Specifically, reactivity is the most critical indicator for judging in vitro biosynthetic reactions. Therefore, selecting appropriate physicochemical parameters of the reaction solution as indicators of its activity is crucial, depending on the type of in vitro biosynthetic reaction. Reactivity can be judged by selecting at least one physicochemical parameter based on different target products; either a single parameter or multiple parameters can be selected. Each physicochemical parameter has a preset activity range corresponding to different target products. Therefore, effective activity means that each physicochemical parameter of the reaction solution corresponding to a preset activity range must be within its corresponding preset activity range. For example, the preset activity ranges might be pH value A, conductivity value B, fluorescence dissolved oxygen value C, and turbidity value D, corresponding to four different physicochemical parameters. Only when each physicochemical parameter of the reaction solution within its preset activity range meets its respective preset activity range is the reaction solution considered effective. Conversely, if any one parameter does not meet the range, the reaction is considered inactive. Furthermore, when determining that the reaction solution has failed, if the preset reaction solution failure time range is no more than 500 seconds, then when the duration of the reaction solution failure does not exceed 500 seconds, the operating module controls the reaction to continue; when the duration of the reaction solution failure exceeds 500 seconds, the operating module determines that the reaction has failed and controls the reaction to terminate.

[0061] In one embodiment, the operating module further regulates the operating conditions based on the reaction liquid temperature parameter and / or ethanol gas concentration parameter: when the reaction liquid temperature parameter and / or ethanol gas concentration parameter do not conform to the preset temperature range and / or preset ethanol concentration range, the operating module activates the corresponding temperature control component and gas regulation component; furthermore, if the duration of non-compliance does not exceed the corresponding preset automatic recovery time range for temperature non-compliance and / or preset automatic recovery time range for ethanol concentration non-compliance, the reaction continues; if the duration of non-compliance exceeds the corresponding preset automatic recovery time range for temperature non-compliance and / or preset automatic recovery time range for gas concentration non-compliance, the reaction stops; even further, if the duration of non-compliance does not exceed the corresponding preset stop time range for temperature non-compliance and / or preset stop time range for ethanol concentration non-compliance, the reaction automatically recovers and continues; if the duration of non-compliance exceeds the corresponding preset stop time range for temperature non-compliance and / or preset stop time range for ethanol concentration non-compliance, it is necessary to determine whether the physicochemical parameters of the reaction liquid conform to the preset activity range; if they conform, the reaction continues; if they do not conform, the reaction fails. Specifically, for example, if the automatic recovery time range for the preset temperature and / or the automatic recovery time range for the preset gas concentration are both no more than 3 seconds, then if the duration of the aforementioned non-compliance is less than 3 seconds, the operation module controls the reaction to continue; if the duration of the aforementioned non-compliance exceeds 3 seconds, the operation module controls the reaction to stop. If the stop time range for the preset temperature and / or the stop time range for the preset gas concentration are both no more than 30 seconds, then if the duration of the aforementioned non-compliance is less than 30 seconds, the operation module controls the reaction to automatically recover; when the duration of the aforementioned non-compliance exceeds 30 seconds, the operation module also needs to determine whether the physicochemical parameters of the reaction solution meet the preset activity range. If they do, the reaction continues; if they do not, the reaction fails.

[0062] In one embodiment, the reaction liquid temperature detection component includes a reaction liquid temperature sensor mounted on the feed pipe 8 near the tank. The reaction liquid temperature sensor sends real-time reaction liquid temperature parameters to the operating module to determine whether temperature control adjustment is necessary. Specifically, the reaction liquid temperature sensor is placed on the pipe closest to the tank 31 to minimize temperature changes caused by heat exchange after the reaction liquid exits the tank 31, thus ensuring measurement accuracy. Additionally, [further details about the component are missing]. Figure 6 As can be seen, during the reaction process, because the liquid detection component 5 is always working, the reaction liquid in the tank 31 is constantly being drawn out from the tank 31 and transported to the sealed detection box 51. The part of the feed pipe 8 that extends into the tank 31 is also used by the liquid sampling pipe of the liquid detection pipeline 54. That is, the reaction liquid temperature sensor near the tank 31 is actually located in the liquid detection pipeline 54. The liquid sampling pipe and the feed pipe 8 share the part that extends into the tank 31. Therefore, the reaction liquid temperature sensor can continuously transmit accurate reaction liquid temperature parameters.

[0063] In one embodiment, the gas detection component 4 includes: a main ventilation pipe 41, a suction pipe 44, and an ethanol gas concentration detection device; such as Figure 7 As shown, the first end of the ventilation main pipe 41 extends into the tank body 31 through the sealed bearing 42, and the port of the second end of the ventilation main pipe 41 is located outside the tank body 31; a portion of the suction pipe 44 is nested inside the ventilation main pipe 41, one end of which extends out of the tank body 31 from the port of the second end of the ventilation main pipe 41 and is connected to the ethanol gas concentration detection device, and the other end extends out from the first end of the ventilation main pipe 41 and is located inside the tank body 31; preferably, the suction pipe 44 extends upward out of the first end; the ethanol gas concentration detection device extracts gas from the tank body through the suction pipe 44 and inputs it into the ethanol gas concentration detection device to detect the ethanol gas concentration in the extracted gas; the ethanol sensor 471 in the ethanol gas concentration detection device sends ethanol gas concentration parameters to the operation module to determine whether to adjust the ethanol gas concentration. Specifically, the main ventilation pipe 41 is first fixed in the hollow part of the slip ring 39 by a sealed bearing 42. When the tank 31 is agitated and rotated, the main ventilation pipe 41 is stationary, and part of the extraction pipe 44 and the feed pipe 8 installed inside the main ventilation pipe 41 are also stationary. The main ventilation pipe 41 constantly exchanges air inside and outside the tank 31, while the extraction pipe 44 extends upward inside the tank 31 to avoid directly drawing in the air that has just been exchanged into the tank 31, which would cause inaccurate detection results from the ethanol gas concentration detection device.

[0064] In one embodiment, such as Figure 8 As shown, the ethanol gas concentration detection device includes a suction component 49, a first suction pipe 46, a transfer detection chamber 47, and a second suction pipe 48 connected in sequence. The transfer detection chamber 47 contains a detection cavity and an ethanol sensor 471. Gas drawn from the suction pipe 44 is introduced through the second suction pipe 48, and the gas detected in the detection cavity is discharged through an outlet, preferably through the suction component 49. Specifically, the portion of the ethanol sensor 471 other than the ethanol concentration detection probe is sealed and separated from the detection cavity by a sealing gasket 472 at the opening of the detection cavity. The suction component 49 is generally a suction motor, also controlled by the operating module. Detecting the gas in the transfer detection chamber during the process of drawing gas from the tank 31 into and out of the sealed chamber avoids the low detection accuracy problem caused by open sampling. Simultaneously, the suction pipe 44 can accelerate the discharge of gas from the tank 31 through the suction component 49, minimizing the ethanol gas concentration below the safe threshold.

[0065] In one embodiment, such as Figure 6As shown, the liquid detection component 5 includes: a sealed detection housing 51, multiple physicochemical sensors 52, and a liquid detection pipeline 54; the detection housing 51 is used to contain the reaction liquid from the tank as a detection sample, and allows the probes of the sensors 52 to be inserted to detect the sample; the liquid detection pipeline 54 connects the detection housing 51 and the tank 31 to form a circulating liquid detection path, and the detection sample enters the detection housing 51 from the liquid detection pipeline 54 and then flows back to the tank 31; the physicochemical sensors 52 send the physicochemical parameters of the reaction liquid to the operation module to determine whether to pause, continue, or end the mixing. Specifically, the liquid detection pipeline 54 also includes: a liquid sampling pipe and a liquid return pipe, a feed three-way valve and a discharge three-way valve, a liquid sampling pump and a liquid return pump. The feed three-way valve is connected to the liquid sampling pipe, the raw material tank of the reaction liquid, and the feed pipe 8 of the reaction tank 31, respectively. The discharge three-way valve is connected to the liquid return pipe, the finished product tank of the reaction liquid, and the discharge pipe of the reaction tank 31, respectively. The liquid sampling pipe takes samples from the reaction tank 31 through the liquid sampling pump, and the liquid return pipe extracts the test sample from the test box 51 through the liquid return pump.

[0066] In one embodiment, such as Figure 6 As shown, the liquid detection assembly 5 also includes a float switch 53, which is located inside the detection chamber 51. When the liquid entering the detection chamber 51 reaches the safe liquid level, the float switch 53 is triggered to shut off the liquid extraction pump and simultaneously start the return pump. The safe liquid level is the preset maximum liquid level; exceeding this level will easily cause the detection chamber 51 to overflow with the sample. Based on the flow rate of the return pump and the volume of reaction liquid that the detection chamber 51 can hold, the running time of the return pump can be preset in the corresponding controller. After the running time is reached, the return pump is shut off, allowing the liquid level in the detection chamber 51 to reach the working liquid level, and then the liquid extraction pump is restarted to extract liquid. The working liquid level is the minimum liquid level that allows each sensor to operate.

[0067] In one embodiment, sensor 52 is a dissolved oxygen sensor, pH sensor, turbidity sensor, conductivity sensor, reaction solution concentration sensor, reaction solution temperature sensor, reaction solution flow sensor, or liquid level sensor. Specifically, sensor 52 can provide parameters such as dissolved oxygen, pH, turbidity, conductivity, reaction solution concentration, and reaction solution flow rate of the reaction solution. The operating module determines the activity of the reaction solution based on one or more physicochemical parameters of the reaction solution and their preset corresponding range values. For example, four physicochemical parameters of the reaction solution—dissolved oxygen, pH, turbidity, and conductivity—are preset as indicators for judging the activity of the reaction solution, and appropriate ranges for the above parameters are specified.

[0068] In one embodiment, such as Figure 9-10As shown, the online quantitative detection device 7 for reaction products includes: a detection chamber 74, an optical device 72, a detection device 71, a sample flow line 76, and a sample detection tube 78. The detection chamber 74 has a detection port. The optical device 72 includes a light source 75, located in the detection chamber 74, which excites the reaction liquid from the container 31 to generate light to be detected. The sample detection tube 78 contains the reaction liquid, at least a portion of which is placed in the detection chamber 74 and is transparent. The reaction liquid in this transparent portion is excited by the light source 75 to generate light to be detected. The detection device 71 collects the light to be detected through the detection port and converts it into an electrical signal, which is then transmitted to the image processing module in the detection device 71. The control device of the in vitro biosynthesis reaction system has a module that converts the electrical signal into calculations to obtain the reaction product content parameters in the reaction liquid and displays them in real time so that the user can promptly grasp the concentration of the reaction products.

[0069] In one example, the quantification module provides the reaction product content parameter to the operation module to determine whether to pause, continue, or terminate the mixing. The sample flow line 76 has an inlet section and an outlet section 77 respectively connected to the sample detection tube 778. The reaction solution enters the sample detection tube 78 from the inlet section and flows through the transparent part, and then exits from the outlet section. Specifically, the inlet section and outlet section 77 of the online quantitative detection device for reaction products can also be independently connected to the reaction vessel 31. For substances that can be excited to generate the light to be measured (such as fluorescence) (such as proteins, nucleic acids, etc.) as synthesis products, or substances that can be excited to generate the light to be measured (such as fluorescence) as synthesis substrates, the concentration of substances that can be excited to generate the light to be measured in the reaction solution can be detected online in real time, so as to understand the reaction process and control the reaction, solving the problem that the concentration of the above substances cannot be detected in real time in the prior art.

[0070] For fluorescence detection, after the reaction solution is excited by the light source 75, the fluorescent groups emit fluorescence. An image is then captured by an optical device 72, such as an optical camera. The detection device 71 can analyze the concentration of the fluorescent substance in the reaction solution based on the fluorescence displayed in the image, thus reflecting the amount of reaction product generated. When the light to be detected is Raman light, the optical device 72 also includes an optical path setup 73 that guides the excitation light to the excitation reaction solution and collects the Raman light generated by the excitation reaction solution. The detection device 71 has a detector that can disperse the collected Raman light through a detection port to convert it into an electrical signal to form a Raman spectrum. Many existing optical path setups 73 for Raman spectroscopy detection can be used.

[0071] In one embodiment, a gas sterilization unit is also included, which includes an ultraviolet lamp mounted outside the reaction vessel assembly and inside a housing assembly 1 surrounding the reaction vessel assembly. The ultraviolet lamp is controlled by the operation module and remains on during the hybridization process.

[0072] In one embodiment, such as Figure 2 As shown, an inspection door 32 is provided on the tank body 31. The inspection door 32 is equipped with a door magnetic sensor, which sends a door magnetic signal to the operation module. The operation module also determines whether the start-up conditions are met based on the door magnetic signal. Specifically, the tank body 31 has a sealing cover assembly at both the front and rear. The front sealing cover assembly includes: a monitoring door 32 at the very front, a roller track 34 fitted onto the tank body 31, and an end cover connecting the monitoring door 32 and the roller track 34. The monitoring door 32 has a transparent viewing window in the center, with the window surface having a 5mm arc convex lens effect.

[0073] In one embodiment, the fully automated in vitro biosynthesis reaction system provided by this utility model further includes a discharge assembly, which has a discharge pipe connected to the tank 31 and a discharge pump connected to the discharge pipe. Specifically, after the reaction is completed, the reaction liquid in the tank 31 is pumped out of the tank 31 by the discharge pump through the discharge pipe.

[0074] In one embodiment, a portion of the discharge pipe extends into the tank 31 via a slip ring. One end of the portion extending into the tank 31 extends radially towards the circumferential edge along the inner wall of the tank 31, while the other end connects to the rotor interface of the slip ring 39. Specifically, as shown... Figure 6 As shown, the portion of the discharge pipe extending into the tank 31 is also used for the return pipe of the liquid detection pipeline 54. The slip ring 39 has at least one passage for transmitting the liquid medium, namely one corresponding interface on the rotor and one on the stator, to connect the inside of the rotating tank 31 and the outside of the stationary tank 31, thereby effectively solving the problem of external connection of the tank 31 in the rotating state. When the tank 31 is self-stirred and rotated, the portion of the discharge pipe extending into the tank 31 also rotates with the reaction tank 1. To ensure that the tank 31 can discharge smoothly, when the tank 31 stops rotating, the portion of the discharge pipe extending into the tank 31 should be at the bottom of the tank 31 to empty the reaction liquid. A positioning part needs to be set to ensure that the stopping angle and position of the tank 31 meet the requirements. The positioning unit is used to sense and position the stopping position of the tank 31. The positioning unit is connected to the hybrid module and the operation module to ensure that the stopping angle of the tank 31 can meet the following requirement: the part of the discharge pipe that extends into the tank 31 and the opening of the pipe extending to the circumferential edge is located at the bottom of the tank 31.

[0075] In one embodiment, the fully automated in vitro biosynthesis reaction system provided by this invention further includes a circulating temperature control component 6, such as... Figure 2-5 As shown, the circulating temperature control assembly 6 includes: a coil 61 fitted tightly to the outer wall of the tank, a constant temperature medium tank 64, and a circulating temperature control pipeline 63 connecting the coil 61 and the constant temperature medium tank 64 to form a circulation path for the medium. Specifically, as shown... Figure 3-4As shown, the coil 61 is tightly wrapped around the outer wall of the tank 31, which is a convenient detachable installation that does not occupy the internal space of the tank 31, thus not interfering with the stirring and reaction process. The circulating temperature control assembly 6 also includes at least one coil clamping strip 62, which is axially pressed onto the coil 61 along the outer wall of the tank 31 and is suitable for being fixed integrally with the outer wall of the tank 31. The coil clamping strip 62 can be a single strip or multiple strips, and its two ends can be welded to the tank 31 integrally. On the one hand, this increases the clamping force between the coil 61 and the tank 31; on the other hand, the coil clamping strip 62 itself can also store some heat, increasing the heat conduction area relative to the tank 31 and improving the heat exchange efficiency. The circulating temperature control assembly 6 also includes a heat insulation material layer, such as sponge 65, which fully covers the outer wall of the coil 61. In one embodiment, wrapping the outer wall of the coil 61 with another layer of heat insulation material can shorten the heating time and save energy. The circulating temperature control assembly 6 also includes a medium circulation pump 66, which can adjust the flow rate of the medium in the circulating temperature control pipeline 63.

[0076] In one embodiment, the operating module further includes a temperature control module, which adjusts the set temperature of the constant temperature medium tank 64 according to the obtained reaction liquid temperature parameters to achieve temperature control. Specifically, the medium in the constant temperature medium tank 64 can be selected from water, heat transfer oil, silicone oil, or ethylene glycol aqueous solution, etc. A medium with a set temperature of T℃ (e.g., 30℃, the specific value can be adapted according to the optimal temperature required by the reaction liquid in the tank 31) circulates between the coil 61 and the constant temperature medium tank 64. The coil 61 itself is also heated to T℃, and the inner ring of the coil 61 contacts the outer wall of the tank 31 for heat exchange, thus heating the tank 31 to T℃ as well. The reaction liquid in the tank 31 is also uniformly heated to T℃ through heat exchange. Therefore, the heating process of the reaction liquid is indirect heating through multiple heat exchanges. The reaction liquid does not directly contact the heating pipe or other active heating devices, preventing local overheating and deactivation of the reaction liquid. Because the liquid medium flowing in coil 61 has a relatively stable temperature, the heating will be very uniform when it fully covers the outer wall of tank 31, which is conducive to rapid and efficient protein synthesis, thereby improving protein yield and activity retention.

[0077] In one embodiment, the coil 61 is formed by folding a metal tube in half and then spirally coiling it to the same diameter. The coil 61 has two parallel ports arranged adjacent to each other, which are the coil inlet and the coil outlet, respectively. Specifically, the pipes of the coil 61 are folded back to form two parallel lines. One line connects to the medium outlet, and its temperature is generally higher. The other line connects to the medium inlet, and its temperature is lower due to heat dissipation along the way. Therefore, arranging the coil inlet and coil outlet adjacent to each other and arranging the two lines in parallel and coiling them together helps to ensure a uniform temperature distribution throughout the coil 61, thereby ensuring uniform heating of the reaction liquid.

[0078] In one embodiment, the coil inlet and outlet are connected to the medium outlet and medium inlet of the circulating temperature control pipeline 63, respectively, via a slip ring 39. Specifically, since the coil 61 is relatively stationary relative to the tank 31, a slip ring 39 is required between the stationary part (circulating temperature control pipeline 63) and the moving part (coil 61) to prevent leakage of the medium liquid when rotating with the tank 31. The slip ring 39 facilitates the switching between stationary and moving parts. This allows the circulating temperature control component 6 of this embodiment to adapt to the rotation of the tank 31.

[0079] The above description is only a preferred embodiment of the present utility model and is not intended to limit the scope of protection of the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the scope of protection of the present utility model.

Claims

1. An automated in vitro biosynthesis reaction system, characterized in that, include: The reactor assembly, mixing assembly, feeding assembly, reactant quantity feedback unit, detection unit, and control device communicating with the aforementioned components, wherein... The reaction vessel assembly has a vessel for containing reaction liquid for in vitro biological reactions; The mixing component is used to achieve mixing of the reaction liquid; The feeding assembly has a feeding pipe connected to the tank body and a feeding pump connected to the feeding pipe; The reaction raw material quantity feedback unit is used to detect the amount of raw material in the tank to obtain raw material quantity parameters; The detection unit is used to detect the reaction liquid and / or the gas produced by the reaction to obtain detection parameters; The control device includes a feeding module, an operation module, and a hybrid module; wherein... The feeding module is used to control the feeding pump to pump the reaction raw materials into the tank through the feeding pipe; The operating module is used to receive the raw material quantity parameters and to send a start signal to the hybrid module when the start conditions are met; The hybrid module is used to start the hybrid component to perform hybridization for a predetermined time and speed after receiving the start signal; The operating module is also communicatively connected to the detection unit to receive the detection parameters and to control the reaction process based on the detection parameters.

2. The reaction system according to claim 1, characterized in that, The inner wall of the tank is uniformly arranged with multiple protruding structures.

3. The reaction system according to claim 1 or 2, characterized in that, The tank is equipped with multiple stirring blades, which may or may not rotate.

4. The reaction system according to claim 3, characterized in that, The non-rotatable stirring blades are arranged at intervals along the inner circumferential wall of the tank.

5. The reaction system according to claim 3, characterized in that, The stirring blade is provided with multiple flow holes, and the spacing between each adjacent flow hole is equal.

6. The reaction system according to claim 1, characterized in that, The hybrid assembly includes: a large gear, a small gear, and a drive unit. The large gear is radially fixed around the outside of the reaction vessel via a gear connecting plate. The small gear meshes with the large gear and is mounted on the rotating shaft of the drive device; The drive unit completes the hybridization by driving the pinion to rotate.

7. The reaction system according to claim 1, characterized in that, The reaction material quantity feedback unit has a weighing sensor installed at the bottom of the tank, and the weighing sensor is communicatively connected to the operating module.

8. The reaction system according to claim 1, characterized in that, The detection unit includes: a reaction condition detection unit and / or a reaction solution parameter detection unit; The reaction condition detection unit includes: a reaction liquid temperature detection component that obtains the reaction liquid temperature parameter by detecting the temperature of the reaction liquid and / or a gas detection component that obtains the ethanol gas concentration parameter by detecting the concentration of ethanol gas generated in the reaction in the tank. The reaction liquid parameter detection unit includes: a liquid detection component for obtaining the physicochemical parameters of the reaction liquid by detecting the physicochemical parameters of the reaction liquid, and / or an online quantitative detection device for reaction products for obtaining the content parameter of reaction products by detecting the content of reaction products generated in the reaction liquid.

9. The reaction system according to claim 8, characterized in that, The operating module is also used to receive the temperature parameters of the reaction liquid and the concentration parameters of the ethanol gas, and to send a start signal to the hybrid module when the start conditions are met.

10. The reaction system according to claim 8 or 9, characterized in that, The reaction liquid temperature detection component includes a reaction liquid temperature sensor installed on the feed pipe near the tank. The reaction liquid temperature sensor sends the reaction liquid temperature parameters in real time to the operating module, which can be used to determine whether to perform temperature control adjustment.

11. The reaction system according to claim 8 or 9, characterized in that, The gas detection assembly includes: a main ventilation pipe, a suction pipe, and an ethanol gas concentration detection device; The first end of the main ventilation pipe extends into the reaction vessel through a sealed bearing, and the second end of the main ventilation pipe is located outside the reaction vessel. The extraction pipe is partially nested inside the main ventilation pipe. One end of the extraction pipe extends out of the reaction vessel from the second end of the main ventilation pipe and is connected to the ethanol gas concentration detection device. The other end of the extraction pipe extends out from the first end of the main ventilation pipe and is located inside the reaction vessel. The ethanol gas concentration detection device extracts gas from the tank through the extraction pipe and inputs it into the ethanol gas concentration detection device to detect the ethanol gas concentration in the extracted gas. The ethanol sensor in the ethanol gas concentration detection device sends the ethanol gas concentration parameter to the operating module, which can be used to determine whether to adjust the ethanol gas concentration.

12. The reaction system according to claim 11, characterized in that, The extraction pipe is partially nested inside the main ventilation pipe. One end of the extraction pipe extends out of the reaction vessel from the second end of the main ventilation pipe and is connected to the ethanol gas concentration detection device. The other end extends upward from the first end of the main ventilation pipe and is located inside the reaction vessel.

13. The reaction system according to claim 11, characterized in that, The ethanol gas concentration detection device includes a suction component, a first suction tube, a transfer detection chamber, and a second suction tube connected in sequence.

14. The reaction system according to claim 13, characterized in that, The transfer detection chamber includes a detection cavity and an ethanol sensor. The gas drawn out by the gas detection assembly is introduced through the second suction tube, and the gas detected by the detection cavity is discharged through the discharge port.

15. The reaction system according to claim 13, characterized in that, The transfer detection chamber includes a detection cavity and an ethanol sensor. The gas drawn out by the suction tube of the gas detection component is introduced from the second suction tube, and the gas detected by the detection cavity is discharged through the suction component.

16. The reaction system according to claim 8 or 9, characterized in that, The liquid detection assembly includes: a sealed detection housing, multiple physicochemical sensors, and liquid detection tubing; The detection box is used to contain the reaction liquid from the tank as a detection sample, and to allow the sensor probe to be inserted to detect the sample. The liquid detection pipeline connects the detection box and the tank to form a circulating liquid detection path, and the detection sample enters the detection box from the liquid detection pipeline and then flows back to the tank; The physicochemical sensor sends the physicochemical parameters of the reaction solution to the operating module, which can be used to determine whether to pause, continue, or end the mixing.

17. The reaction system according to claim 16, characterized in that, The sensors are dissolved oxygen sensors, pH sensors, turbidity sensors, conductivity sensors, reaction solution concentration sensors, reaction solution temperature sensors, reaction solution flow sensors, or liquid level sensors.

18. The reaction system according to claim 8 or 9, characterized in that, The online quantitative detection equipment for reaction products includes: a detection chamber, an optical device, a detection device, a sample flow pipeline, and a sample detection tube; The testing chamber is equipped with a testing port; The optical device includes a light source located in the detection chamber, and the light source is used to excite the reaction liquid from the tank to generate light to be detected. The sample detection tube is used to contain the reaction solution, at least a portion of the sample detection tube is placed in the detection chamber and the at least a portion is a transparent portion, and the reaction solution is excited by the light source in the transparent portion to generate the light to be detected; The detection device collects the light to be detected through the detection port and converts it into an electrical signal; the electrical signal transmits the reaction product content parameter to the operation module, which can be used to determine whether to pause, continue or end the mixing. The sample flow pipeline has an inlet section and an outlet section that are respectively connected to the sample detection tube. The reaction solution enters the sample detection tube from the inlet section and flows through the transparent part, and then exits from the outlet section.

19. The reaction system according to claim 1, characterized in that, It also includes a gas sterilization unit, which includes an ultraviolet lamp installed outside the reaction vessel assembly and inside a housing assembly surrounding the reaction vessel assembly. The ultraviolet lamp is controlled by the operation module and remains on during the hybridization process.

20. The reaction system according to claim 1, characterized in that, The tank is equipped with an inspection door, which is fitted with a door magnetic sensor. The door magnetic sensor sends a door magnetic signal to the operating module, which can determine whether the start-up conditions are met based on the door magnetic signal.

21. The reaction system according to claim 1, characterized in that, It also includes a discharge assembly, which has a discharge pipe connected to the tank body and a discharge pump connected to the discharge pipe.

22. The reaction system according to claim 21, characterized in that, A portion of the discharge pipe extends into the tank body through a slip ring. One end of the portion extending into the tank body extends radially toward the circumferential edge along the inner wall of the tank body, while the other end is connected to the rotor interface of the slip ring.

23. The reaction system according to claim 10, characterized in that, It also includes a circulating temperature control component, which includes: a coil sleeved on the outer wall of the tank and tightly fitted therewith, a constant temperature medium tank, and a circulating temperature control pipeline that connects the coil and the constant temperature medium tank to form a circulating passage for the medium.

24. The reaction system according to claim 23, characterized in that, The operating module also includes a temperature control module, which adjusts the set temperature of the constant temperature medium tank according to the obtained reaction liquid temperature parameters to achieve temperature control.

25. The reaction system according to claim 24, characterized in that, The coil is formed by folding a metal tube in half and then spirally coiling it with equal diameter. The coil has two parallel ports arranged adjacent to each other, which are the coil inlet and the coil outlet, respectively.

26. The reaction system according to claim 24, characterized in that, The coil inlet and the coil outlet are connected to the medium outlet and the medium inlet in the circulating temperature control pipeline respectively via slip rings.

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