Intestinal flora extraction and concentration device

Abstract

The utility model discloses a kind of intestinal flora extraction, concentration equipment, including stirring barrel, intestinal bacteria filter device for extracting bacteria liquid in stirring barrel, bacteria liquid collection bottle and concentration disc for circulating concentration of bacteria liquid in the bacteria liquid collection bottle that has been collected in order along material supply direction are sequentially communicated, intestinal bacteria filter device is composed of at least two sealed boxes of identical structure and circular section, any two adjacent sealed boxes are connected by two pipelines, each sealed box is built-in filter screen of different mesh, all filter screens in sealed box are arranged from coarse to fine according to the order of filtration, each sealed box is connected into a whole by support, an extraction peristaltic pump is equipped on the pipeline connected to the inlet of each sealed box, a concentration peristaltic pump is equipped on the pipeline connecting bacteria liquid collection bottle outlet to concentration disc inlet. The equipment of the utility model adopts cross-flow extraction disc stack filter to filter and extract intestinal bacteria liquid, with simple structure, high extraction efficiency and reduced filter screen clogging problem.

Description

Technical Field

[0001] This utility model relates to an intestinal flora extraction and concentration device, belonging to the field of medical equipment technology. Background Technology

[0002] Gut microbiota transplantation (GMT) is defined as the transplantation of functional microbiota from the feces of healthy individuals into the intestines of a patient, restoring a new balance of gut microbiota and achieving therapeutic effects for both intestinal and extraintestinal diseases. This technique has achieved excellent results in treating Clostridium difficile infection (CDI), inflammatory bowel disease (IBD), and irritable bowel syndrome (IBS). The gut microecological environment plays a crucial role in human nutrition, metabolism, and immunity. Therefore, standardized fecal microbiota separation systems are of great significance.

[0003] Currently, the domestic market is flooded with various equipment, including those using static extraction followed by centrifugal concentration and those using single-stage cross-flow extraction followed by cross-flow concentration, but none are entirely satisfactory. For example, stirring fails to disperse intestinal contents, resulting in insufficient dispersion of intestinal bacteria in the liquid; static extraction followed by centrifugal concentration leads to significant loss of intestinal bacterial diversity due to centrifugation; cross-flow technology is used in FMT to extract intestinal bacteria from feces, but the flow plate structure of single-stage cross-flow extraction devices is more complex than that of static extraction. Previous technologies all used a single mesh, addressing clogging issues by increasing the mesh area, but the fecal capacity of a single mesh is too small, only 70-100 grams of donor feces per cycle, significantly lower than the normal 200-300 grams of feces from a healthy donor, resulting in high preparation costs (multiple sets of consumables) and significantly reduced efficiency (long preparation time). Summary of the Invention

[0004] The technical problem to be solved by this invention is: how to increase the amount of donor feces prepared in a single-stage crossflow extraction and reduce the problem of filter clogging.

[0005] To solve the above-mentioned technical problems, the present invention provides an intestinal flora extraction and concentration device, characterized in that it includes a stirring tank connected sequentially along the material supply direction, an intestinal flora filtration device for extracting bacterial solution from the stirring tank, a bacterial solution collection bottle, and a concentration plate for circulating and concentrating the bacterial solution in the collected bacterial solution collection bottle. The intestinal flora filtration device consists of at least two sealed boxes with identical structures and circular cross-sections stacked together. Any two adjacent sealed boxes are connected by two pipelines. Each sealed box contains a filter screen with different mesh sizes. All the filter screens in the sealed boxes are arranged from coarse to fine according to the filtration sequence. All the sealed boxes are connected to a support to form a whole. Each sealed box inlet is connected to a pipeline equipped with an extraction peristaltic pump, and a concentration peristaltic pump is installed on the pipeline connecting the inlet of the concentration plate to the outlet of the bacterial solution collection bottle. All the sealed boxes are cross-flow plates.

[0006] Preferably, the intestinal bacteria filtration device is a two-stage cross-flow extraction disc stacked filter, consisting of two sealed boxes, namely a first-stage sealed box and a second-stage sealed box. A first extraction peristaltic pump is installed on the pipeline connected to the inlet of the first-stage sealed box, and a second extraction peristaltic pump is installed on the pipeline connected to the inlet of the second-stage sealed box. The inlet of the first extraction peristaltic pump is connected to the outlet of the mixing tank of the mixing tank through a pipeline, and the outlet of the first extraction peristaltic pump is connected to the inlet of the first-stage filtrate of the first-stage sealed box through a pipeline. The return outlet of the mixing tank is connected to the outlet of the first-stage filtrate of the first-stage sealed box through a pipeline. The outlet of the first-stage permeate of the first-stage sealed box is connected to the inlet of the second extraction peristaltic pump through a pipeline. The outlet of the second extraction peristaltic pump is connected to the inlet of the second-stage filtrate of the second-stage sealed box through a pipeline. The outlet of the second-stage filtrate of the second-stage sealed box is connected to the return outlet of the first-stage permeate of the first-stage sealed box, and the outlet of the second-stage permeate of the second-stage sealed box is connected to the inlet of the bacterial culture bottle of the bacterial culture collection bottle through a pipeline.

[0007] Preferably, the first extraction peristaltic pump and the second extraction peristaltic pump are both two-channel driven peristaltic pumps; the first extraction peristaltic pump and the second extraction peristaltic pump are respectively connected to a controller that controls the opening and closing of the first extraction peristaltic pump and the second extraction peristaltic pump.

[0008] Preferably, the bacterial liquid collection bottle is a graduated medical plastic bottle with three ports on its cap: a bacterial liquid inlet, a bacterial liquid outlet, and a bacterial liquid return port. The bacterial liquid inlet connects to the outlet of the intestinal bacteria filtration device. The bacterial liquid outlet and bacterial liquid return port are for circulating and concentrating the extracted bacterial liquid. The bacterial liquid outlet is located inside the cap and also has a port connected to a flexible tube extending to the bottom of the bacterial liquid collection bottle. The bacterial liquid outlet and bacterial liquid return port are connected to a concentration tray via flexible tubes, and the flexible tube connected to the bacterial liquid outlet is equipped with a concentration peristaltic pump.

[0009] Preferably, the outlet of the bacterial culture bottle is connected to the inlet of the concentration peristaltic pump via a hose, the outlet of the concentration peristaltic pump is connected to the inlet of the concentration plate via a hose, and the return port of the bacterial culture bottle is connected to the outlet of the concentration plate via a hose.

[0010] Preferably, the bacterial liquid collection bottle is equipped with a second liquid level sensor to detect whether the liquid level in the bacterial liquid collection bottle is higher than a specified position, and the concentration peristaltic pump is connected to a controller that controls the opening and closing of the concentration peristaltic pump according to the signal given by the second liquid level sensor, and the controller is connected to the second liquid level sensor.

[0011] Preferably, the top cover of the mixing tank is connected to an automatic servo water replenishment device. The automatic servo water replenishment device includes a water replenishment tank, a water replenishment peristaltic pump, and a controller. The water replenishment tank and the mixing tank are connected by a pipeline. One end of the pipeline extends into the water replenishment tank near the bottom, and the other end of the pipeline is connected to the mixing tank liquid replenishment port of the mixing tank. The water replenishment peristaltic pump is provided on the pipeline. A first liquid level sensor is provided in the mixing tank to measure whether the liquid level in the mixing tank is lower than the marked position. The water replenishment peristaltic pump is connected to a controller that controls the opening and closing of the water replenishment peristaltic pump according to the signal given by the first liquid level sensor. The controller is connected to the first liquid level sensor.

[0012] Preferably, the mixing tank is a bottom-mounted "toilet-style" motor-driven mixing tank; the cross-section of the mixing tank perpendicular to the center is a trapezoid with a larger top and a smaller bottom.

[0013] Preferably, the concentration tray is a structure that adopts the cross-flow principle and is composed of a plate and frame membrane module.

[0014] To ensure the standardized fecal microbiota separation system can be truly applied clinically, this invention provides a solution of "extraction disc stacked filtration cross-flow extraction + bacterial solution bottle (intermediate connection) + single concentration disc circulating cross-flow concentration," achieving truly high efficiency, large capacity, and full automation in the extraction and concentration of bacterial solution. This thoroughly perfects standardized fecal microbiota preparation, making it simple, clean, and efficient. Therefore, the entire operation is carried out in a closed space, thus ensuring anaerobic and temperature-controlled processes.

[0015] This invention significantly improves several aspects of the extraction of intestinal bacteria from donor feces. The stirring is carried out by a bottom motor, which completely pulverizes the donor feces and allows the intestinal bacteria to be fully distributed in the liquid. The extraction adopts a cross-flow disc filtration system, with a pump driving the inlet of each filter screen to reduce the problem of filter screen clogging. The filter screens are arranged from coarse to fine, achieving fine filtration, ultrafiltration, and ultrafiltration. The concentration adopts the cross-flow principle, and the concentration disc automatically circulates and concentrates the bacterial solution extracted from the bacterial solution bottle until a certain bacterial solution concentration is reached.

[0016] This invention employs a two-stage cross-flow disc superimposed filtration extraction method, which significantly simplifies the structure of the cross-flow disc and greatly improves the filtration efficiency. This invention can extract up to 300 grams of donor feces in a single extraction, completely covering the normal stool volume of an adult. Compared with existing technologies, the device of this invention, using a cross-flow extraction disc superimposed filtration method to extract intestinal flora, truly achieves a simple structure, high extraction efficiency, and relatively stable diversity of extracted intestinal flora, thus extracting as many intestinal bacteria as possible from the donor feces.

[0017] This invention employs a multi-layer (two or more) cross-flow disc (i.e., sealed box) stacked filtration technology, where multiple screens carry the donor feces to be filtered. This greatly increases the amount of feces extracted in a single filtration, saving time and costs. Because of this multi-screen stacked filtration method, the structure of the cross-flow discs can be designed to be compact. With this method, the amount of feces extracted is large, and because of the circulating cross-flow, the extraction is very clean and efficient.

[0018] The present invention uses a bacterial solution bottle as a transition between the intestinal bacteria filtration device and the concentration plate. In this way, extraction and concentration can be carried out simultaneously. After completion, the bacterial solution bottle contains the extracted and concentrated bacterial solution, which greatly facilitates and simplifies the operation of the preparation personnel.

[0019] Based on the previous net weight of the feces, the controller will automatically set the amount of saline solution injected through the program. When the amount of feces is large, after the extraction module is started, the program will automatically add water to the mixing tank according to the liquid output of the mixing tank to prevent the concentration in the mixing tank from rising suddenly and clogging the bottom screen of the mixing tank, which would cause the extraction to fail. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of a device for extracting and concentrating intestinal flora.

[0021] in:

[0022] P1: Water replenishment peristaltic pump; P2: First extraction peristaltic pump; P3: Second extraction peristaltic pump; P4: Concentration peristaltic pump; J: Stirring tank; B1: Water replenishment tank; B2: Bacterial liquid collection bottle; N: Concentration plate; G: Secondary cross-flow extraction plate stacked filter;

[0023] 1: Mixing tank replenishment port; 2: Mixing tank return port; 3: Mixing tank outlet; 4: First-stage filtrate outlet; 5: First-stage filtrate inlet; 6: First-stage permeate return port; 7: First-stage permeate outlet; 8: Second-stage filtrate outlet; 9: Second-stage filtrate inlet; 10: Second-stage permeate outlet; 11: Bacterial solution bottle inlet; 12: Bacterial solution bottle outlet; 13: Bacterial solution bottle return port; 14: Concentration tray outlet; 15: Concentration tray inlet. Detailed Implementation

[0024] To make this utility model more apparent and understandable, preferred embodiments are described in detail below with reference to the accompanying drawings.

[0025] This invention provides an intestinal flora extraction and concentration device for separating, extracting, and concentrating intestinal flora from feces, such as... Figure 1 As shown, it includes a mixing tank J connected sequentially along the material supply direction, an intestinal bacteria filtration device for extracting bacterial liquid in the mixing tank J, a bacterial liquid collection bottle B2, and a concentration plate N for circulating and concentrating the bacterial liquid in the collected bacterial liquid collection bottle B2.

[0026] The mixing tank J is a bottom-mounted "toilet-style" motor-driven agitator. The cross-section of the mixing tank J perpendicular to the center is a trapezoid with a larger top and a smaller bottom. The top cover of the mixing tank J is connected to an automatic servo water replenishment device. Based on the monitoring program, the controller automatically performs the corresponding amount of water replenishment according to various factors such as the amount of feces and the amount of liquid output in the mixing tank J. The purpose is to keep the concentration of the suspension in the mixing tank J relatively constant within the effective extraction time, and to prevent sudden changes in concentration from causing clogging of the bottom screen of the mixing tank J, thereby affecting the extraction efficiency. The automatic servo water replenishment device includes a water replenishment tank B1, a water replenishment peristaltic pump P1, and a controller. The water replenishment tank B1 and the mixing tank J are connected by a pipeline. One end of the pipeline extends into the water replenishment tank B1 near the bottom, and the other end of the pipeline is connected to the mixing tank replenishment port 1 of the mixing tank J. The water replenishment peristaltic pump P1 is installed on the pipeline. The controller is connected to the water replenishment peristaltic pump P1 and the first liquid level sensor. When the first liquid level sensor (i.e., the liquid level gauge) in the mixing tank J detects that the liquid in the mixing tank J is lower than the marked position, the water replenishment peristaltic pump P1 is started to draw brine from the water replenishment tank B1 into the mixing tank J until the marked position is reached.

[0027] The intestinal bacteria filtration device employs a two-stage cross-flow extraction disc stack filter G (containing two sealed boxes) or a multi-stage cross-flow extraction disc stack filter (containing multiple sealed boxes). In this embodiment, the intestinal bacteria filtration device employs a two-stage cross-flow extraction disc stack filter G. The two-stage cross-flow extraction disc stack filter G consists of a first-stage sealed box and a second-stage sealed box. The filter screens within the multiple sealed boxes are arranged in order of filtration, from coarse (large mesh size) to fine (small mesh size), thus dividing the process into fine filtration and ultrafiltration. For the entry of liquid into each stage of the filter screen, a peristaltic pump is used (specifically, a first extraction peristaltic pump P2 and a second extraction peristaltic pump P3) to ensure the smooth flow of the filtrate.

[0028] Because a two-stage filtration system is used, the cross-flow extraction plate differs from the concentration plate and is smaller in size. The stirring tank J and the two-stage cross-flow extraction plate filter G are connected by two 7mm latex tubes. The first extraction peristaltic pump P2 drives the suspension from the stirring tank J into the first-stage sealed box of the two-stage cross-flow extraction plate filter G for circulating cross-flow filtration extraction. After the first-stage sealed box completes the circulating filtration extraction, it is again connected by two 7mm latex tubes. The second extraction peristaltic pump P3 drives the first-stage filtered permeate into the second-stage sealed box of the two-stage cross-flow extraction plate filter G for circulating cross-flow filtration extraction again. Finally, the intestinal bacteria permeate is output from the outlet of the second-stage sealed box of the two-stage cross-flow extraction plate filter G through a single 7mm latex tube to the bacterial collection bottle B2, waiting to be concentrated.

[0029] The secondary cross-flow extraction disc stacked filter G consists of two identical circular sealed boxes (made of medical-grade plastic) stacked together using two latex tubing tubes (7mm outer diameter, 3mm inner diameter) through a double-chain structure. Each sealed box contains a different stainless steel filter screen with mesh sizes ranging from coarse to fine, specifically 140 mesh and 280 mesh. The two sealed boxes are connected as a whole by a bracket. The pipeline between the mixing tank outlet 3 and the first-stage filtrate inlet 5 is driven by the first extraction peristaltic pump P2, and the pipeline between the first-stage permeate outlet 7 and the second-stage filtrate inlet 9 is driven by the second extraction peristaltic pump P3. Both the first extraction peristaltic pump P2 and the second extraction peristaltic pump P3 are two-channel driven peristaltic pumps.

[0030] Specifically, the inlet of the first extraction peristaltic pump P2 is connected to the outlet 3 of the mixing tank J via a pipeline; the outlet of the first extraction peristaltic pump P2 is connected to the inlet 5 of the first-stage filtrate of the first-stage sealed box via a pipeline; the return outlet 2 of the mixing tank J is connected to the outlet 4 of the first-stage filtrate of the first-stage sealed box via a pipeline; the outlet 7 of the first-stage permeate of the first-stage sealed box is connected to the inlet of the second extraction peristaltic pump P3 via a pipeline; the outlet of the second extraction peristaltic pump P3 is connected to the inlet 9 of the second-stage filtrate of the second-stage sealed box via a pipeline; the outlet 8 of the second-stage filtrate of the second-stage sealed box is connected to the return outlet 6 of the first-stage permeate of the first-stage sealed box; and the outlet 10 of the second-stage permeate of the second-stage sealed box is connected to the inlet 11 of the bacterial culture collection bottle B2 via a pipeline.

[0031] Bacterial fluid collection bottle B2 is a 1000ml graduated medical plastic bottle with three ports on its cap: bacterial fluid inlet 11, bacterial fluid outlet 12, and bacterial fluid return port 13. The bacterial fluid outlet 12 and bacterial fluid return port 13 are for circulating and concentrating the extracted bacterial fluid. The bacterial fluid outlet 12 is located inside the cap and also has a tube connected to it, which extends to the bottom of the bacterial fluid collection bottle B2. The purpose is to ensure that the bacterial fluid in the entire bacterial fluid collection bottle B2 is circulated and concentrated.

[0032] The bacterial solution outlet 12 and return outlet 13 of the bacterial solution bottle are connected to the concentration filter plate (i.e., concentration plate N) via flexible hoses. The hose connected to the bacterial solution outlet 12 is driven by a concentration peristaltic pump P4, which drives the bacterial solution from the bacterial solution collection bottle B2 to the concentration plate N for circulation and concentration until a certain bacterial solution concentration is reached, thus completing the bacterial solution preparation. When the second liquid level sensor in the bacterial solution collection bottle B2 detects that there is liquid in the bacterial solution collection bottle B2 above the specified level, the concentration peristaltic pump P4 is activated.

[0033] Specifically, the outlet 12 of the bacterial culture bottle is connected to the inlet of the concentration peristaltic pump P4 via a hose, the outlet of the concentration peristaltic pump P4 is connected to the concentration plate inlet 15 of the concentration plate N via a hose, and the return port 13 of the bacterial culture bottle is connected to the concentration plate outlet 14 of the concentration plate N via a hose.

[0034] The concentration plate N adopts the cross-flow principle and is composed of a plate and frame membrane module. The plate and frame module is the first large-scale ultrafiltration system to be applied. This design originated from the filtration concept and is similar to the design of a conventional plate and frame filter press. The membrane is placed on a porous support plate, and filter paper is placed on the support plate. Two porous support plates are stacked together to form a feed channel space, which constitutes a membrane module (concentration filter plate).

[0035] The system comprises a first liquid level sensor, a second liquid level sensor, a water replenishment peristaltic pump P1, a first extraction peristaltic pump P2, a second extraction peristaltic pump P3, and a concentration peristaltic pump P4, all connected to a controller. The controller controls the starting and stopping of these pumps. The controller receives signals from the first and second liquid level sensors. It controls the start of the water replenishment peristaltic pump P1 based on the signal from the first liquid level sensor, and controls the start of the concentration peristaltic pump P4 based on the signal from the second liquid level sensor. The controller can be a PLC controller.

[0036] The usage process of this utility model is as follows:

[0037] Case 1: Donor D1, fecal mass 150 grams.

[0038] Place 150 grams of feces in mixing tank J, inject 800 ml of physiological saline, start the stirring motor in mixing tank J, and stir for 3 to 5 minutes. Turn on the first extraction peristaltic pump P2 and the second extraction peristaltic pump P3, which starts the two-stage cross-flow extraction disc filter G. The first extraction peristaltic pump P2 drives the suspension through the latex tube from the mixing tank outlet 3 to the first-stage filtrate inlet 5, and from the first-stage filtrate outlet 4 to the mixing tank return outlet 2, performing a first-stage cross-flow circulation extraction. The second extraction peristaltic pump P3 drives the suspension through the latex tube from the first-stage permeate outlet 7 to the second-stage filtrate inlet 9. The bacterial solution is extracted through a two-stage cross-flow circulation process, from the outlet 8 of the second-stage filtrate to the return outlet 6 of the first-stage permeate, to complete the extraction. The bacterial solution flows from the outlet 10 of the second-stage permeate through the inlet 11 of the bacterial solution bottle into the bacterial solution bottle B2, yielding the extracted bacterial solution. The monitoring program (controller) automatically starts the concentration peristaltic pump P4, which draws the bacterial solution collected in the bacterial solution collection bottle B2 out through the outlet 12 of the bacterial solution bottle and into the concentration plate N through the inlet 15 of the concentration plate for concentration. After concentration, the solution flows back from the outlet 14 of the concentration plate through the return outlet 13 of the bacterial solution bottle into the bacterial solution collection bottle B2. After circulating for 30 to 40 minutes, the concentration is completed, and the entire preparation is finished.

[0039] Specifically, the bacterial solution entering from the first-stage filtrate inlet 5 is filtered by the filter screen in the first-stage sealed box and then splits into two streams. One stream of filtrate exits from the first-stage filtrate outlet 4 and flows through a pipe (i.e., a latex tube) to the return port 2 of the mixing tank, entering the mixing tank J. The other stream of permeate comes from the first-stage permeate outlet 7. The bacterial solution from the first-stage permeate outlet 7 flows to the second-stage filtrate inlet 9 and enters the second-stage sealed box. After being filtered by the filter screen in the second-stage sealed box, it also splits into two streams. One stream of filtrate exits from the second-stage filtrate outlet 8 and flows through a pipe (i.e., a latex tube) to the return port 6 of the first-stage permeate, entering the first-stage sealed box. The other stream of permeate comes from the second-stage permeate outlet 10 and flows through the bacterial solution bottle inlet 11 into the bacterial solution collection bottle B2.

[0040] Case 2: Donor D2, fecal mass of 250 grams.

[0041] 250 grams of feces were placed in the mixing tank J, and 800 ml of physiological saline was injected. The stirring motor in the mixing tank J was started and stirred for 3 to 5 minutes. The first extraction peristaltic pump P2 and the second extraction peristaltic pump P3 were turned on, which started the two-stage cross-flow extraction disc filter G. The first extraction peristaltic pump P2 drove the suspension through the latex tube from the mixing tank outlet 3 to the first-stage filtrate inlet 5, and from the first-stage filtrate outlet 4 to the mixing tank return outlet 2, performing a first-stage cross-flow circulation extraction. The second extraction peristaltic pump P3 drove the suspension through the latex tube from the first-stage permeate outlet 7 to the second-stage filtrate inlet 9, and from the second-stage filtrate outlet 8 to the first-stage permeate return outlet 6, performing a second-stage cross-flow circulation extraction, completing the extraction of bacterial solution. The bacterial solution flowed from the second-stage permeate outlet 10 through the bacterial solution bottle inlet 11 into the bacterial solution bottle B2, obtaining the extracted bacterial solution. Simultaneously, based on the donor fecal mass M, the monitoring program activates the water replenishment peristaltic pump P1 to replenish the corresponding amount of water in the mixing tank J, preventing sudden changes in the concentration of the suspension in the mixing tank J from clogging the filter screen. Upon completion of bacterial solution extraction, the monitoring program automatically activates the concentration peristaltic pump P4, drawing the bacterial solution collected in the bacterial solution collection bottle B2 from the outlet 12 through the inlet 15 into the concentration plate N for concentration. After concentration, the solution flows back from the outlet 14 of the concentration plate to the return outlet 13 of the bacterial solution bottle into the bacterial solution collection bottle B2. After circulating for 30-40 minutes, concentration is complete, and the entire preparation process is finished.

[0042] Specifically, the bacterial solution entering from the first-stage filtrate inlet 5 is filtered by the filter screen in the first-stage sealed box and then splits into two streams. One stream of filtrate exits from the first-stage filtrate outlet 4 and flows through a pipe (i.e., a latex tube) to the return port 2 of the mixing tank, entering the mixing tank J. The other stream of permeate comes from the first-stage permeate outlet 7. The bacterial solution from the first-stage permeate outlet 7 flows to the second-stage filtrate inlet 9 and enters the second-stage sealed box. After being filtered by the filter screen in the second-stage sealed box, it also splits into two streams. One stream of filtrate exits from the second-stage filtrate outlet 8 and flows through a pipe (i.e., a latex tube) to the return port 6 of the first-stage permeate, entering the first-stage sealed box. The other stream of permeate comes from the second-stage permeate outlet 10 and flows through the bacterial solution bottle inlet 11 into the bacterial solution collection bottle B2.

Claims

1. An intestinal flora extraction and concentration device, characterized in that, The system includes a mixing tank (J) connected sequentially along the material supply direction, an intestinal bacteria filtration device for extracting bacterial solution from the mixing tank (J), a bacterial solution collection bottle (B2), and a concentration plate (N) for circulating and concentrating the bacterial solution in the collected bacterial solution collection bottle (B2). The intestinal bacteria filtration device consists of at least two identical sealed boxes stacked together with circular cross-sections. Any two adjacent sealed boxes are connected by two pipelines. Each sealed box contains a filter screen with different mesh sizes. All the filter screens in the sealed boxes are arranged from coarse to fine according to the filtration sequence. All the sealed boxes are connected to a support to form a whole. Each sealed box inlet is connected to a pipeline with an extraction peristaltic pump. A concentration peristaltic pump (P4) is installed on the pipeline connecting the inlet of the concentration plate (N) to the outlet of the bacterial solution collection bottle (B2). All the sealed boxes are cross-flow plates.

2. The intestinal flora extraction and concentration equipment as described in claim 1, characterized in that, The intestinal bacteria filtration device is a two-stage cross-flow extraction disc stacked filter (G), consisting of two sealed boxes, namely a first-stage sealed box and a second-stage sealed box. A first extraction peristaltic pump (P2) is installed on the pipeline connected to the inlet of the first-stage sealed box, and a second extraction peristaltic pump (P3) is installed on the pipeline connected to the inlet of the second-stage sealed box. The inlet of the first extraction peristaltic pump (P2) is connected to the outlet (3) of the stirring tank (J) through a pipeline, and the outlet of the first extraction peristaltic pump (P2) is connected to the inlet (5) of the first-stage filtrate of the first-stage sealed box through a pipeline. The stirring tank return of the stirring tank (J) is... The liquid outlet (2) is connected to the first-stage filtrate outlet (4) of the first-stage sealed box through a pipeline. The first-stage permeate outlet (7) of the first-stage sealed box is connected to the inlet of the second extraction peristaltic pump (P3) through a pipeline. The outlet of the second extraction peristaltic pump (P3) is connected to the second-stage filtrate inlet (9) of the second-stage sealed box through a pipeline. The second-stage filtrate outlet (8) of the second-stage sealed box is connected to the first-stage permeate return outlet (6) of the first-stage sealed box. The second-stage permeate outlet (10) of the second-stage sealed box is connected to the bacterial culture bottle inlet (11) of the bacterial culture collection bottle (B2) through a pipeline.

3. The intestinal flora extraction and concentration equipment as described in claim 2, characterized in that, The first extraction peristaltic pump (P2) and the second extraction peristaltic pump (P3) are both two-channel driven peristaltic pumps; the first extraction peristaltic pump (P2) and the second extraction peristaltic pump (P3) are respectively connected to the controller that controls the opening and closing of the first extraction peristaltic pump (P2) and the second extraction peristaltic pump (P3).

4. The intestinal flora extraction and concentration equipment as described in claim 1, characterized in that, The bacterial liquid collection bottle (B2) is a graduated medical plastic bottle with three ports on its cap: bacterial liquid bottle inlet (11), bacterial liquid bottle outlet (12), and bacterial liquid bottle return port (13). The bacterial liquid bottle inlet (11) is connected to the outlet of the intestinal bacteria filtration device. The bacterial liquid bottle outlet (12) and bacterial liquid bottle return port (13) are outlets and return ports for circulating and concentrating the extracted bacterial liquid. The bacterial liquid bottle outlet (12) is located inside the cap and also has a port. A flexible tube is connected to this port and extends to the bottom of the bacterial liquid collection bottle (B2). The bacterial liquid bottle outlet (12) and bacterial liquid bottle return port (13) are respectively connected to the concentration plate (N) through flexible tubes. The flexible tube connected to the bacterial liquid bottle outlet (12) is equipped with a concentration peristaltic pump (P4).

5. The intestinal flora extraction and concentration equipment as described in claim 4, characterized in that, The outlet (12) of the bacterial liquid bottle is connected to the inlet of the concentration peristaltic pump (P4) through a hose, the outlet of the concentration peristaltic pump (P4) is connected to the inlet (15) of the concentration plate (N) through a hose, and the return port (13) of the bacterial liquid bottle is connected to the outlet (14) of the concentration plate (N) through a hose.

6. The intestinal flora extraction and concentration equipment as described in claim 1, characterized in that, The bacterial liquid collection bottle (B2) is equipped with a second liquid level sensor to detect whether the liquid in the bacterial liquid collection bottle (B2) is higher than a specified position. The concentration peristaltic pump (P4) is connected to a controller that controls the opening and closing of the concentration peristaltic pump (P4) according to the signal given by the second liquid level sensor. The controller is connected to the second liquid level sensor.

7. The intestinal flora extraction and concentration equipment as described in claim 1, characterized in that, The top cover of the mixing tank (J) is connected to an automatic servo water replenishment device. The automatic servo water replenishment device includes a water replenishment tank (B1), a water replenishment peristaltic pump (P1), and a controller. The water replenishment tank (B1) and the mixing tank (J) are connected by a pipeline. One end of the pipeline extends into the water replenishment tank (B1) near the bottom, and the other end of the pipeline is connected to the mixing tank liquid replenishment port (1) of the mixing tank (J). The water replenishment peristaltic pump (P1) is installed on the pipeline. A first liquid level sensor is installed in the mixing tank (J) to measure whether the liquid in the mixing tank (J) is lower than the marked position. The water replenishment peristaltic pump (P1) is connected to a controller that controls the opening and closing of the water replenishment peristaltic pump (P1) according to the signal given by the first liquid level sensor. The controller is connected to the first liquid level sensor.

8. The intestinal flora extraction and concentration equipment as described in claim 1, characterized in that, The mixing tank (J) is a bottom-mounted "toilet-style" motor-driven mixing tank; the cross-section of the mixing tank (J) perpendicular to the center is a trapezoid with a larger top and a smaller bottom.

9. The intestinal flora extraction and concentration equipment as described in claim 1, characterized in that, The concentration plate (N) is a structure that adopts the cross-flow principle and is composed of plate and frame membrane modules.

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