A sterile environment maintaining device for cell culture
By introducing a dual ultraviolet sterilization system and a through-structure design into the cell culture device, the problems of poor sealing and inconvenient operation were solved, achieving stable maintenance of the sterile environment and improving the success rate of cell culture.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- ZUNYI BEIKE RONGHUI LIFE TECH CO LTD
- Filing Date
- 2025-07-17
- Publication Date
- 2026-06-19
AI Technical Summary
In existing cell culture devices, poor sealing at the connection between the operating components and the housing, as well as inconvenience in handling pipettes, make the sterile environment susceptible to contamination, affecting the success rate and stability of cell culture.
A sterile environment maintenance device was designed, comprising a maintenance chamber, a pre-sterilization chamber, and an operating rod. It employs a dual ultraviolet sterilization system and connects the pre-sterilization chamber and the maintenance chamber through a through structure to ensure that pipettes can be directly inserted into the maintenance chamber after sterilization. The pipettes are then clamped by a sealed operating rod, forming a closed operating process. Combined with a humidity sensor and a humidifier, a suitable culture environment is maintained.
It effectively reduces the risk of bacterial contamination, ensures the stability of the sterile environment and the success rate of cell culture, reduces the intrusion of external air, and improves the ease of operation and cell viability.
Smart Images
Figure CN224378042U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, and specifically to a sterile environment maintenance device for cell culture. Background Technology
[0002] In the field of cell culture technology, existing cell culture devices typically maintain a sterile environment by installing components such as ultraviolet germicidal lamps and temperature and humidity sensors inside the incubator. Some devices also have a pre-sterilization box on the side of the incubator to perform preliminary sterilization on items entering the incubator. They are also equipped with operating gloves or operating levers to allow for operations such as adding reagents without directly opening the incubator.
[0003] However, there are still obvious shortcomings in the existing technology: on the one hand, during the operation of traditional culture devices, such as when adding reagents by clamping pipettes with the operating rod, the poor sealing effect at the connection between the operating rod and the incubator may allow outside air to enter and introduce bacteria; on the other hand, the through-structure design of the pre-sterilization box and the incubator is not perfect, and there may be cases of incomplete sterilization during the sterilization process. Moreover, the existing operating rod structure design is inconvenient to operate when clamping pipettes, which increases the risk of contamination of the sterile environment and affects the success rate and stability of cell culture. Utility Model Content
[0004] The present invention aims to provide a sterile environment maintenance device for cell culture, so as to solve the problems of poor sealing at the connection between the operating components and the box body and inconvenience in picking up and handling pipettes in existing cell culture devices, which lead to easy contamination of the sterile environment.
[0005] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a sterile environment maintenance device for cell culture, comprising a maintenance box, a chassis, a pre-sterilization box, and multiple operating rods. The maintenance box has a fixed plate at its inner top, and each fixed plate is equipped with a first ultraviolet lamp. A motor is located at the inner bottom of the maintenance box, with its output shaft facing upwards and connected to the chassis. Multiple culture dishes are connected to the chassis. The pre-sterilization box is connected to the outside of the side wall of the maintenance box, with its middle section extending into the maintenance box. A second ultraviolet lamp is located at the inner top of the pre-sterilization box. A placement plate is connected to the middle of the pre-sterilization box, with locking blocks connected to both ends of the placement plate. The locking blocks are located at both ends of the pre-sterilization box. Multiple pipettes are connected to the placement plate. One end of each operating rod penetrates the side wall of the maintenance box and is located inside the maintenance box. The operating rods are used to grip the pipettes to add reagents.
[0006] The working principle of this invention is as follows: A first ultraviolet lamp is mounted on a fixed plate at the top of the maintenance chamber, continuously sterilizing the culture environment with ultraviolet light. A motor at the bottom of the maintenance chamber drives the output shaft to rotate, causing the chassis and connected culture dishes to rotate at a uniform speed, ensuring that the cells in the culture dishes receive uniform light and reagent treatment. A pre-sterilization chamber is connected to the maintenance chamber through a side wall. A second ultraviolet lamp at the top of the pre-sterilization chamber pre-sterilizes the pipettes placed on the placement plate. The locking blocks at both ends of the placement plate are fixed to the ports of the pre-sterilization chamber to ensure the stability of the pipettes during sterilization. When reagents need to be added, the operator uses an operating rod that penetrates the side wall of the maintenance chamber to grasp the pre-sterilized pipettes, moves the pipettes above the culture dishes, and adds reagents to the rotating culture dishes. The entire process does not require opening the maintenance chamber. Through dual ultraviolet sterilization and a closed operating structure, a sterile environment is continuously maintained.
[0007] The beneficial effects of this invention are as follows: 1. The first ultraviolet lamp at the top of the maintenance chamber and the second ultraviolet lamp at the top of the pre-sterilization chamber form a dual sterilization system, which can respectively sterilize the culture environment inside the maintenance chamber and the pipettes inside the pre-sterilization chamber, effectively reducing the risk of bacterial contamination. 2. The pre-sterilization chamber is connected to the maintenance chamber through a through-hole structure, allowing the pipettes to be directly gripped into the maintenance chamber by the operating rod after pre-sterilization, avoiding the intrusion of external air caused by traditional opening of the chamber, and reducing the possibility of contamination of the sterile environment. 3. The operating rod is set through the side wall of the maintenance chamber, allowing pipette gripping and reagent addition without opening the maintenance chamber. Combined with the overall structural design of the device, a closed aseptic operation process is formed, effectively maintaining the stability of the sterile environment inside the chamber.
[0008] Furthermore, a humidity sensor and a humidifier are connected to the inner wall of the maintenance chamber, and the humidity sensor and the humidifier are electrically connected. The humidity sensor and the humidifier are electrically connected, which can monitor the humidity in the maintenance chamber in real time and automatically start the humidifier to adjust, ensuring a suitable humidity environment required for cell culture, avoiding the impact of unsuitable humidity on cell activity, and improving the culture success rate.
[0009] Furthermore, the base plate has multiple grooves that conform to the shape of the culture dish. The grooves on the base plate, adapted to the shape of the culture dish, can firmly fix the culture dish in place, preventing it from shifting during base plate rotation or operation, ensuring stable position of the culture dish, facilitating precise operation and uniform cell culture.
[0010] Furthermore, a sealing sleeve connects the operating lever to the maintenance chamber. This sealing sleeve effectively enhances the seal at the connection point, preventing outside air and bacteria from entering the maintenance chamber through gaps, thus avoiding contamination of the sterile environment and ensuring the reliability of the cell culture environment.
[0011] Furthermore, the locking block is connected to a handle located on the outside. This externally located handle allows operators to easily push and pull the locking block, moving the placement plate within the pre-sterilization box. This facilitates the replacement or retrieval of straws without frequently opening the pre-sterilization box, reducing the risk of external contamination and improving operational convenience.
[0012] Furthermore, the operating lever includes a connecting rod, a handle, and a V-clamp. The handle is connected to one end of the connecting rod and is located externally. The other end of the connecting rod is connected to the closed end of the V-clamp. The handle and the V-clamp are electrically connected. The operating lever employs a structure of a connecting rod, a handle, and a V-clamp, with the handle electrically connected to the V-clamp. The operator can control the opening and closing of the V-clamp from the outside via the handle, allowing for precise gripping of pipettes. The electrical connection design enables automated control, providing flexible operation and reducing contamination from manual contact, thus improving the accuracy and efficiency of reagent addition.
[0013] Furthermore, the operating lever includes a connecting rod, a linkage rod, a V-shaped clamp, and a pull block. The closed end of the V-shaped clamp is connected to the linkage rod, and the middle of both ends of the V-shaped clamp are rotatably connected to the ends of the connecting rod. The open end of the V-shaped clamp is located to the outside. One end of the connecting rod is connected to a spacer block, and the linkage rod passes through the spacer block and connects to the pull block. A spring is connected to the bottom end of the pull block and the top end of the spacer block. The pull block drives the linkage rod to control the closing of the V-shaped clamp, and the spring returns to its original position to open it. This structure utilizes the principle of mechanical linkage, allowing the operator to easily control the V-shaped clamp to pick up and drop the straw from the outside using the pull block. The operation is effortless and stable. The spring ensures the reliability of the clamping action and reduces the risk of the straw falling or becoming contaminated due to inconvenient operation. Attached Figure Description
[0014] Figure 1 This is a schematic diagram of the structure of a sterile environment maintenance device for cell culture according to the present invention;
[0015] Figure 2 for Figure 1 Top view;
[0016] Figure 3 This is a schematic diagram of the operating lever of a sterile environment maintenance device for cell culture according to the present invention;
[0017] Figure 4 for Figure 3 A schematic diagram of the working state.
[0018] The reference numerals in the accompanying drawings include: handle 1, connecting rod 2, sealing sleeve 3, holding box 4, fixing plate 5, first ultraviolet lamp 6, humidifier 7, humidity sensor 8, pre-sterilization box 9, placement plate 10, second ultraviolet lamp 11, locking block 12, handle 13, straw 14, base 15, motor 16, petri dish 17, V-clamp 18, slide 19, spacer block 20, pull block 21, connecting rod 22, and spring 23. Detailed Implementation
[0019] The following detailed description illustrates the specific implementation method:
[0020] Example 1 is basically as shown in the appendix. Figures 1-2 As shown: A sterile environment maintenance device for cell culture includes a maintenance chamber 4, a base 15, a pre-sterilization chamber 9, and two operating levers. The rear side wall of the maintenance chamber 4 is a movable door. A fixing plate 5 is fixedly connected to the top of the maintenance chamber 4, and each fixing plate 5 is equipped with a first ultraviolet lamp 6. The bottom of the maintenance chamber 4 has two sliding grooves 19. A humidity sensor 8 and a humidifier 7 are fixedly connected to the inner side wall of the maintenance chamber 4. The humidity sensor 8 and the humidifier 7 are electrically connected. A motor 16 is fixedly connected to the bottom of the maintenance chamber 4. The output shaft of the motor 16 is vertically upward and fixedly connected to the center of the base 15. The base 15 has nine grooves, and culture dishes 17 are slidably connected in the grooves. The pre-sterilization chamber 9 is fixedly connected to the outside of the right side wall of the maintenance chamber 4, and the middle of the pre-sterilization chamber 9 is open. Inside the maintenance box 4, a second ultraviolet lamp 11 is installed on the top of the pre-disinfection box 9. A placement plate 10 is slidably connected to the middle of the pre-disinfection box 9. Two locking blocks 12 are vertically fixed to both ends of the placement plate 10. The two locking blocks 12 are located at the two ports of the pre-disinfection box 9, and the size of the locking blocks 12 is adapted to the size of the ports of the pre-disinfection box 9. The bottom end of the locking block 12 is slidably connected to two sliding grooves 19. A handle 13 is fixedly connected to the right locking block 12. The handle 13 is located outside. Eight suction tubes 14 are slidably connected through the placement plate 10. The operating rod includes a connecting rod 2, a handle 1, and a V-shaped clamp 18. The handle 1 is fixedly connected to one end of the connecting rod 2 and is located outside. The other end of the connecting rod 2 is fixedly connected to the closed end of the V-shaped clamp 18. The handle 1 and the V-shaped clamp 18 are electrically connected.
[0021] Example 2 is attached. Figure 3-4 As shown: The operating lever includes a connecting rod 2, a linkage rod 22, a V-shaped clamp 18, and a pull block 21. The closed end of the V-shaped clamp 18 is fixedly connected to the linkage rod 22. The middle of both ends of the V-shaped clamp 18 is rotatably connected to the end of the connecting rod 2. The open end of the V-shaped clamp 18 is located to the outside. A spacer block 20 is fixedly connected to one end of the connecting rod 2. The linkage rod 22 passes through the spacer block 20 and is fixedly connected to the pull block 21. A spring 23 is fixedly connected to the bottom end of the pull block 21 and the top end of the spacer block 20.
[0022] The specific implementation process is as follows: The cell culture dish 17 is placed and fixed in the groove of the base plate 15. After the power is turned on, the first ultraviolet lamp 6 at the top of the maintenance chamber 4 sterilizes the environment inside the chamber, and the second ultraviolet lamp 11 at the top of the pre-sterilization chamber 9 pre-sterilizes the pipettes 14 on the placement plate 10. The operator pulls the locking block 12 through the handle 13 to remove the placement plate 10 along with the pipettes 14 from the port of the pre-sterilization chamber 9. After placing a new pipette 14, it is pushed back, and the locking block 12 secures both ends of the pre-sterilization chamber 9. The humidity sensor 8 monitors the humidity inside the maintenance chamber 4 in real time. When the humidity is insufficient, the electrically connected humidifier 7 automatically starts to adjust. When reagents need to be added, the operator holds the handle 1 of the operating lever. If it is an electrical connection structure, pressing the switch on handle 1 energizes the V-clamp 18 to close and clamp the pre-sterilized pipette 14. If it is a linkage rod 22 structure, pulling down the pull block 21 drives the V-clamp 18 to close and clamp the pipette 14 via the linkage rod 22. The spring 23 returns to its original position after releasing the pull block 21, causing the V-clamp 18 to open. The operating lever passes through the sealing sleeve 3 on the side wall of the maintenance box 4, ensuring that outside air cannot enter when clamping the pipette 14. The pipette 14 is moved into the maintenance box 4 through the through-structure between the pre-sterilization box 9 and the maintenance box 4. The motor 16 is started, and the output shaft drives the base 15 to rotate, and the culture dish 17 rotates accordingly. The operator controls the pipette 14 to evenly add reagents to the culture dish 17 through the operating lever. After adding the reagents, the pipette 14 is placed back into the pre-sterilization box 9 or removed. Throughout the process, the first and second ultraviolet lamps 11 continue to work to maintain a sterile environment.
[0023] The above descriptions are merely embodiments of this utility model, and common knowledge regarding specific structures and characteristics is not elaborated upon here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of this utility model, and these should also be considered within the scope of protection of this utility model. These modifications will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.
Claims
1. A sterile environment maintaining device for cell culture, characterized by: The device includes a maintenance box, a chassis, a pre-sterilization box, and multiple operating levers. The maintenance box has a fixed plate at its top interior, with a first ultraviolet lamp mounted on each plate. A motor is located at the bottom interior of the maintenance box, with its output shaft facing upwards and connected to the chassis. Multiple culture dishes are connected to the chassis. The pre-sterilization box is connected to the outside of the side wall of the maintenance box, extending through the middle into the maintenance box. A second ultraviolet lamp is located at the top interior of the pre-sterilization box. A placement plate is connected to the middle of the pre-sterilization box, with locking blocks at both ends. Multiple pipettes are connected to the placement plate. One end of each operating lever penetrates the side wall of the maintenance box and is located inside the box. The operating levers are used to grip the pipettes to add reagents.
2. The sterile environment maintaining device for cell culture according to claim 1, wherein: A humidity sensor and a humidifier are connected to the inner wall of the maintenance box, and the humidity sensor and the humidifier are electrically connected.
3. The sterile environment maintenance device for cell culture according to claim 2, characterized in that: The base plate has multiple grooves that are adapted to the shape of the petri dish.
4. The sterile environment maintenance device for cell culture according to claim 3, characterized in that: A sealing sleeve connects the operating lever to the holding box.
5. The sterile environment maintenance device for cell culture according to claim 4, characterized in that: The card block is connected to a handle, which is located on the outside.
6. The sterile environment maintenance device for cell culture according to claim 5, characterized in that: The operating lever includes a connecting rod, a handle, and a V-shaped clamp. The handle is connected to one end of the connecting rod and is located outside the external environment. The other end of the connecting rod is connected to the closed end of the V-shaped clamp. The handle and the V-shaped clamp are electrically connected.
7. The sterile environment maintenance device for cell culture according to claim 5, characterized in that: The operating lever includes a connecting rod, a linkage rod, a V-shaped clamp, and a pull block. The closed end of the V-shaped clamp is connected to the linkage rod, and the middle of both ends of the V-shaped clamp is rotatably connected to the end of the connecting rod. The open end of the V-shaped clamp is located to the outside. One end of the connecting rod is connected to a spacer block. The linkage rod passes through the spacer block and is connected to the pull block. The bottom end of the pull block is connected to the top end of the spacer block with a spring.