Spill-resistant cell counting plate

Abstract

The utility model relates to cell counting plate technical field, especially prevent the overflow formula cell counting plate of spilling, including substrate, barrier groove, the top of substrate is provided with the apron, one side of substrate is provided with the observation platform, the top of observation platform is provided with the counting square for observing the count, the edge of observation platform is provided with the barrier groove for storing liquid exhaust, the apron is provided with the liquid injection port for injecting test solution to the observation platform, and the liquid injection port is located at the edge of observation platform one side, the utility model discloses the barrier groove is set up around the observation platform and is stored to the test solution of injection is cut off, and the apron is set up the liquid injection port directly and injects the test solution on the top of observation platform, and the substrate and apron are fixed by fixed assembly and are fixed and limited, prevent the position of liquid injection port from producing deviation, avoid the test solution siphon hydraulic transmission caused by injecting from the apron originally, and along with the apron overflow, and directly injecting the test solution from the liquid injection port can effectively avoid the generation of the bubble caused by uneven distribution of test solution.

Description

Technical Field

[0001] This utility model relates to the field of cell counting plate technology, and in particular to an overflow-proof cell counting plate. Background Technology

[0002] A cell counting chamber is a tool used to count cells, commonly found in biological and medical research. It typically consists of a specially designed glass slide and coverslips. The slide has specific graduations and grids to divide the counting area.

[0003] In the process of using a cell counting chamber, first place a coverslip on top of the slide. The area of ​​the coverslip is often smaller than that of the slide. Then, use a syringe or other pipette to inject the test solution into the slide from the edge of the coverslip, so that the test solution fills the space between the coverslip and the slide. After that, place the coverslip and the slide together under a microscope for observation and counting.

[0004] The method of injecting liquid from the edge of the coverslip is prone to causing the test liquid to overflow along the coverslip and generate air bubbles under the siphon hydraulic transmission. Furthermore, this method makes it difficult for the test liquid to fill all the space between the coverslip and the slide, resulting in air bubbles. When air bubbles are within the observation grid, they will have a negative impact on the observation. In addition, the existing cell counting chamber does not have a fixed coverslip and slide, and the coverslip is prone to moving when the slide is moved, which makes it impossible for the coverslip and slide to fit tightly together. This further causes the test liquid to overflow with the coverslip, resulting in air bubbles. Utility Model Content

[0005] To overcome the problems of injecting liquid from the edge of the coverslip, which easily causes the test solution to overflow along the coverslip and generate air bubbles under the siphon hydraulic transmission, and also makes it difficult for the test solution to fill all the space between the coverslip and the slide, thus generating air bubbles, when the air bubbles are within the observation grid area, they will have an adverse effect on the observation. In addition, the existing cell counting chamber does not fix the coverslip and slide in place, and the coverslip is easily moved when the slide is moved, which makes it impossible for the coverslip and slide to fit tightly together, further causing the test solution to overflow with the coverslip and generate air bubbles.

[0006] The technical solution of this utility model is as follows: an anti-overflow cell counting plate, including a base plate and a barrier groove. A cover plate is provided on the top of the base plate, and an observation stage is provided on one side of the base plate. A counting grid for observing and counting is provided on the top of the observation stage. A barrier groove for storing liquid, venting gas and preventing overflow is provided on the edge of the observation stage. An injection port for injecting test liquid into the observation stage is provided on the cover plate. The injection port is located at one edge of the observation stage.

[0007] Preferably, fixing holes are provided on both sides of the cover plate and the base plate for fixing, and the fixing holes penetrate the base plate and the cover plate.

[0008] Preferably, the cover plate has an exhaust port for venting and draining liquid, and the exhaust port is located on the side of the observation platform away from the liquid injection port.

[0009] Preferably, a positioning post is provided on one side of the cover plate, and the position of the positioning post is consistent with the position of the positioning hole.

[0010] Preferably, the diameter of the positioning hole is the same as that of the positioning post, and the positioning post is fixedly connected to the cover plate.

[0011] Preferably, the height of the upper surface of the observation stage is lower than the height of the upper surface of the substrate, and the height difference is consistent with the general standard specifications, forming a cavity with the cover plate that is connected and communicates with the barrier groove.

[0012] I. This cell counting chamber is made of biologically harmless transparent polyester material with an ultraviolet transmittance of over 80%. Through polyester modification, the counting chamber has good hydrophilic properties.

[0013] Second, the observation stage and bottom of the counting plate adopt a concave design to minimize the thickness of the light source and observation area, thereby reducing material refraction and light loss and better restoring the true morphology of cells.

[0014] Third, this cell counting chamber adopts a high-precision manufacturing process. The cavity formed by the observation stage and the cover plate has a depth of 100um and a manufacturing precision of ±2um. Through precision control, the cell counting CV can be reduced to ≤2%.

[0015] IV. The depth of the cavity formed by the observation stage and cover plate of this cell counting chamber can be designed to be between 30um and 400um, depending on the different cell diameters.

[0016] 5. The counting grid within the cavity formed by the observation platform and cover plate of this cell counting chamber can be equipped with a grid design structure for counting bovine cells and urine sediment to meet the counting functions of different microstructures such as bacteria, microorganisms, and sperm.

[0017] VI. The base plate of this cell counting plate is designed to be relatively thick, ranging from 0.5 to 2 mm, while the cover plate is relatively thin, ranging from 0.1 to 1 mm.

[0018] Preferably, the counting grid uses the standard Tommy or Healy format.

[0019] The beneficial effects of this utility model are:

[0020] By creating barrier grooves around the observation platform to store and isolate the injected test solution, and by creating an injection port on the cover plate to directly inject the test solution above the observation platform, the fixing assembly fixes and limits the substrate and the cover plate to prevent the position of the injection port from shifting. This avoids the test solution siphon hydraulic transmission caused by injecting from the cover plate and overflows along the cover plate. Furthermore, injecting the test solution directly from the injection port can effectively avoid the generation of bubbles due to uneven distribution of the test solution. Attached Figure Description

[0021] Figure 1 The diagram shown is a three-dimensional structural schematic of this utility model;

[0022] Figure 2 The diagram shown is a schematic representation of the split structure of this utility model.

[0023] Figure 3 The diagram shown is a three-dimensional structural schematic of the cover plate of this utility model;

[0024] Figure 4 The diagram shown is a three-dimensional cross-sectional view of the present invention.

[0025] Explanation of reference numerals in the attached drawings: 1. Cover plate; 2. Base plate; 3. Positioning hole; 101. Injection port; 102. Exhaust port; 201. Observation platform; 202. Barrier groove; 301. Positioning post. Detailed Implementation

[0026] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0027] Please see Figures 1-4 This utility model provides an embodiment of an anti-overflow cell counting plate, including a base plate 2 and a barrier groove 202. A cover plate 1 is provided above the base plate 2, and an observation platform 201 is provided on one side of the base plate 2. A counting grid for observation and counting is provided above the observation platform 201. The edge of the observation platform 201 is provided with a barrier groove 202 for storing liquid, venting gas and preventing overflow. The cover plate 1 is provided with a liquid injection port 101 for injecting test liquid into the observation platform 201. The liquid injection port 101 is located at one edge of the observation platform 201. By opening the barrier groove 202 around the observation platform 201 to store and isolate the injected test liquid, and opening the liquid injection port 101 on the cover plate 1 to directly inject the test liquid above the observation platform 201, the uneven distribution of test liquid can effectively avoid the generation of bubbles caused by uneven distribution of test liquid.

[0028] Please see Figure 2 In this embodiment, positioning holes 3 for fixing are provided on both sides of the cover plate 1 and the base plate 2. The positioning holes 3 penetrate the base plate 2 and the cover plate 1. The cover plate 1 is provided with an exhaust port 102 for venting and draining liquid. The exhaust port 102 is located on the side of the observation table 201 away from the liquid injection port 101. The positioning holes 3 are used to fix the base plate 2 and the cover plate 1 with the positioning post 301. The exhaust port 102 is used to balance the internal and external air pressure of the chamber between the base plate 2 and the cover plate 1 so that the test liquid can enter smoothly.

[0029] In use, first place the cover plate 1 on top of the substrate 2 and insert the positioning post 301 of the cover plate 1 into the positioning hole 3 to connect and fix the substrate 2 and the cover plate 1. Then, use a pipette to inject the test liquid from the injection port 101 into the space formed above the observation stage 201 and the cover plate 1. Excess test liquid will enter the barrier tank 202 and be stored to prevent the test liquid from overflowing into the space between the substrate 2 and the cover plate 1 under capillary action and siphon effect. Then, place the substrate 2 and the cover plate 1 together under a microscope for observation and counting.

[0030] Through the above steps, the injected test solution is stored and isolated by opening a barrier groove 202 around the observation platform 201, and the test solution is directly injected into the top of the observation platform 201 by opening a liquid injection port 101 on the cover plate 1. Directly injecting the test solution from the liquid injection port 101 can effectively avoid the generation of bubbles caused by uneven distribution of the test solution.

Claims

1. An anti-spill flow cell counting plate comprising a base plate (2); characterised in that: It also includes a barrier groove (202), a cover plate (1) is provided above the substrate (2), an observation platform (201) is provided on one side of the substrate (2), a counting grid for observation and counting is provided above the observation platform (201), a barrier groove (202) for storing liquid, venting gas and preventing overflow is provided on the edge of the observation platform (201), and a liquid injection port (101) for injecting test liquid into the observation platform (201) is provided on the cover plate (1), and the liquid injection port (101) is located at the edge of one side of the observation platform (201).

2. The anti-overflow cell counting plate according to claim 1, wherein: A positioning hole (3) for fixing is provided on one side of the substrate (2), and the positioning hole (3) penetrates the substrate (2).

3. The anti-overflow cell counting plate of claim 1, wherein: The cover plate (1) has an exhaust port (102) for venting and draining liquid. The exhaust port (102) is located on the side of the observation platform (201) away from the injection port (101).

4. The anti-overflow cell counting plate according to claim 2, wherein: A positioning post (301) is provided on one side of the cover plate (1), and the position of the positioning post (301) is consistent with the position of the positioning hole (3).

5. The anti-overflow cell counting plate according to claim 4, wherein: The diameter of the positioning hole (3) is the same as that of the positioning post (301), and the positioning post (301) is fixedly connected to the cover plate (1).

6. The anti-overflow cell counting plate of claim 1, wherein: The counting grid uses the standard Tommy or Healy format.

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