Blood cell circulation culture device

Abstract

This utility model relates to the field of cell culture technology, and more particularly to a blood cell circulation culture device, including an insulated box and a base. The insulated box is mounted on the upper side of the base, and a circulation culture mechanism is provided inside the insulated box. The circulation culture mechanism includes a protective sleeve, a circulation hose, a peristaltic pump, and a spiral culture tube. The protective sleeve is located inside the insulated box, and a transparent spiral culture tube is located inside the protective sleeve. The upper and lower ends of the spiral culture tube extend from the upper and lower ends of the protective sleeve, respectively. Both ends of the spiral culture tube are connected to the transparent circulation hose through sealing connectors. A peristaltic pump is mounted on the right side of the insulated box through a fixing bracket, and the right section of the circulation hose is detachably wound inside the peristaltic pump. This utility model drives the circulation of culture medium to accurately simulate the blood flow environment in the human body, providing blood cells with growth conditions closer to those in vivo, thereby improving the quality and effect of blood cell culture.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and more specifically, to a blood cell circulation culture device. Background Technology

[0002] Cell culture is a technique that simulates the physiological environment in vivo by taking living cells from a living organism and placing them in a suitable artificial environment to allow them to grow, multiply, and maintain specific functions. It is one of the fundamental technologies in modern biology, medicine, and biotechnology, and is widely used in basic research, drug development, disease model construction, biopharmaceuticals, tissue engineering, and many other fields.

[0003] In modern biomedical research and cell therapy, blood cell culture is crucial. Currently, most blood cell culture devices employ static culture methods, placing blood cells in a culture medium within a container where they grow in a relatively static environment. However, human blood is constantly circulating, and the static culture environment differs significantly from the actual blood flow environment in the human body. This difference prevents blood cells from obtaining physiological conditions such as substance exchange and shear forces similar to those in vivo during culture, thus affecting their growth, differentiation, metabolism, and other physiological functions. Consequently, the cultured blood cells exhibit significant differences in biological characteristics and function compared to in vivo blood cells, leading to impaired cell function or low culture efficiency. Therefore, there is an urgent need for a circulating culture device that can simulate the human blood flow environment to address these issues. Utility Model Content

[0004] The purpose of this invention is to provide a blood cell circulation culture device that precisely simulates the blood flow environment in the human body by driving the culture medium to circulate, thereby providing blood cells with growth conditions closer to those in the body and improving the quality and effect of blood cell culture.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0006] A blood cell circulation culture device includes an insulated box and a base, wherein the insulated box is installed on the upper side of the base, and a circulation culture mechanism is provided inside the insulated box;

[0007] The circulating culture mechanism includes a protective sleeve, a circulating hose, a peristaltic pump, and a spiral culture tube. The protective sleeve is located inside the insulated box, and a transparent spiral culture tube is located inside the protective sleeve. The upper and lower ends of the spiral culture tube extend from the upper and lower ends of the protective sleeve, respectively. The upper and lower ends of the spiral culture tube are connected to the transparent circulating hose through sealing connectors. The peristaltic pump is installed on the right side of the insulated box through a fixing bracket, and the right section of the circulating hose is detachably wrapped inside the peristaltic pump.

[0008] Furthermore, two sets of supports are provided on the inner left side wall of the insulated box, and the protective sleeve is detachably clamped on the two sets of supports;

[0009] The bracket consists of a fixed support rod and a U-shaped hanger. One end of the fixed support rod is fixedly connected to the inner wall of the insulation box, and the other end is provided with a U-shaped hanger. The specifications of the U-shaped hanger are adapted to the specifications of the protective cylinder. Two sets of positioning baffles are provided on the ring surface of the protective cylinder, and the diameter of the positioning baffles is the same as the diameter of the U-shaped hanger.

[0010] Furthermore, a hose valve is installed near the upper end of the circulation hose.

[0011] Furthermore, the spiral culture tube has an air inlet head on its upper annular surface that communicates with its interior, and the upper end of the air inlet head is inclined outward.

[0012] Furthermore, the protective sleeve is made of medical-grade transparent plastic, and multiple sets of through grooves are evenly formed on its annular surface.

[0013] Furthermore, an openable and closable insulated box door is installed on the front side of the insulated box body.

[0014] Furthermore, the base has a partition inside, a constant temperature electric heater is installed in the right area of ​​the partition, a blower is installed in the left area of ​​the partition, a ventilation slot is opened on the partition, the blower's air outlet is connected to the ventilation slot, a heat dissipation vent is opened on the left side of the base corresponding to the blower, and multiple sets of air outlets are evenly opened on the upper surface of the base.

[0015] Furthermore, a main controller is provided on the front side of the blower, and a control panel is mounted on the front surface of the base. All electrical components are connected to the main controller via wires, and the main controller is electrically connected to the control panel via wires.

[0016] Furthermore, an adjustable exhaust port is installed on the top of the insulated box.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. This utility model uses a circulating culture device to drive the culture medium to circulate, which can simulate the blood flow environment in the human body and provide blood cells with growth conditions that are closer to those in the body. This allows blood cells to obtain material exchange conditions similar to those in the body during the culture process, which is beneficial to cell growth and metabolism, thereby improving the quality and effect of blood cell culture.

[0019] 2. In this invention, an air inlet head is provided on the upper annular surface of the spiral culture tube, which is connected to the interior of the spiral culture tube. The upper end of the air inlet head is inclined outward. The air inlet head facilitates the entry of air into the spiral culture tube, providing the necessary oxygen for cell culture in the culture medium.

[0020] 3. The protective sleeve of this utility model is made of medical transparent plastic, which makes it easy to observe the flow of culture medium in the internal culture tube directly through the protective sleeve. Multiple sets of through grooves are evenly opened on its annular surface to facilitate the entry of hot air from the heat preservation box into the inside of the protective sleeve and to fully contact the spiral culture tube, thereby heating the culture medium in the spiral culture tube. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0022] Figure 2 This is a schematic diagram of the structure of this utility model from another angle.

[0023] Figure 3 This is a schematic diagram of the internal structure of the insulation box in this utility model.

[0024] Figure 4 This is a partial sectional view of the present invention.

[0025] Figure 5 This is a schematic diagram of the circulating culture mechanism in this utility model.

[0026] Figure 6 This is a partial structural diagram of the circulating culture mechanism in this utility model.

[0027] Figure 7 This is a schematic diagram of the internal structure of the protective sleeve in this utility model.

[0028] Figure 8 This is a schematic diagram of the support structure in this utility model.

[0029] In the diagram: 1. Insulation chamber; 2. Insulation chamber door; 3. Base; 4. Control panel; 5. Adjustable exhaust port; 6. Heat dissipation port; 7. Circulation culture mechanism; 71. Protective sleeve; 72. Support; 721. Fixed support rod; 722. U-shaped hanger; 73. Circulation hose; 74. Peristaltic pump; 75. Fixed frame; 76. Positioning baffle; 77. Through groove; 78. Hose valve; 79. Sealing connector; 710. Air inlet; 711. Spiral culture tube; 8. Air outlet; 9. Blower; 10. Main controller; 11. Partition; 12. Connecting ventilation slot; 13. Constant temperature electric heater. Detailed Implementation

[0030] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0031] Example:

[0032] like Figures 1 to 8 As shown, a blood cell circulation culture device includes an insulated box 1 and a base 3. The insulated box 1 is installed on the upper side of the base 3, and a circulation culture mechanism 7 is provided inside the insulated box 1.

[0033] The circulating culture mechanism 7 includes a protective sleeve 71, a circulating hose 73, a peristaltic pump 74, and a spiral culture tube 711. The protective sleeve 71 is located inside the insulated chamber 1. A transparent spiral culture tube 711 is located inside the protective sleeve 71, with its upper and lower ends extending from the upper and lower ends of the protective sleeve 71. Both ends of the spiral culture tube 711 are connected to the transparent circulating hose 73 via sealing connectors 79. The peristaltic pump 74 is installed on the right side of the insulated chamber 1 via a fixing bracket 75. The right section of the circulating hose 73 is detachably wound around the peristaltic pump 74. This design solves the problem that existing blood cell culture devices use static culture methods, which cannot simulate the human blood flow environment, thus affecting the physiological functions of cell growth, differentiation, and metabolism. This results in cultured blood cells having significant differences in biological characteristics and functions compared to in vivo blood cells, leading to impaired cell function or low culture efficiency. This device drives the circulation of the culture medium to accurately simulate the human blood flow environment, providing blood cells with growth conditions closer to those in vivo, thereby improving the quality and effectiveness of blood cell culture.

[0034] In this embodiment, two sets of brackets 72 are provided on the inner left side wall of the insulated box 1, and the protective sleeve 71 is detachably clamped on the two sets of brackets 72.

[0035] The support frame 72 consists of a fixed support rod 721 and a U-shaped hanger 722. One end of the fixed support rod 721 is fixedly connected to the inner wall of the insulation box 1, and the other end is equipped with a U-shaped hanger 722. The specifications of the U-shaped hanger 722 are compatible with the specifications of the protective sleeve 71. Two sets of positioning baffles 76 are provided on the annular surface of the protective sleeve 71, and the diameter of the positioning baffles 76 is the same as the diameter of the U-shaped hanger 722. This design, through the two sets of supports 72, facilitates the clamping and fixing of the protective sleeve 71, making it easy to fix the protective sleeve 71 inside the insulation box 1.

[0036] In this embodiment, a hose valve 78 is installed near the upper end of the circulation hose 73. The hose valve 78 facilitates the control of the opening and closing of the circulation hose 73 and can also adjust the flow rate of the culture medium in the circulation hose 73.

[0037] In this embodiment, an air inlet 710 communicating with the interior is provided on the upper annular surface of the spiral culture tube 711, with the upper end of the air inlet 710 inclined outward. The air inlet 710 facilitates the entry of air into the spiral culture tube 711, providing the necessary oxygen for cell culture in the culture medium.

[0038] In this embodiment, the protective sleeve 71 is made of medical transparent plastic, which makes it easy to directly observe the flow of culture medium in the internal culture tube through the protective sleeve 71. Multiple sets of through grooves 77 are evenly opened on its annular surface, which makes it easy for hot air in the heat preservation box 1 to enter the interior of the protective sleeve 71 and fully contact the spiral culture tube 711 to heat the culture medium in the spiral culture tube 711.

[0039] In this embodiment, an openable and closable insulated box door 2 is installed on the front side of the insulated box 1. The insulated box door 2 can seal the insulated box 1, which can effectively reduce the heat exchange between the inside of the insulated box 1 and the external environment and reduce the heat loss rate. At the same time, the openable and closable insulated box door 2 allows the staff to easily operate and maintain the circulating culture mechanism 7 inside the insulated box 1.

[0040] In this embodiment, a partition 11 is provided inside the base 3. A constant-temperature electric heater 13 is installed in the right area of ​​the partition 11, and a blower 9 is installed in the left area of ​​the partition 11. A ventilation slot 12 is provided on the partition 11, and the air outlet of the blower 9 is connected to the ventilation slot 12. A heat dissipation vent 6 is provided on the left side of the base 3 corresponding to the blower 9, and multiple sets of air outlets 8 are evenly provided on the upper surface of the base 3. This design, through the use of the constant-temperature electric heater 13 and the blower 9, facilitates the heating of the internal space of the insulation box 1, so that the culture medium in the circulating culture mechanism 7 can be maintained at a suitable temperature.

[0041] In this embodiment, a main controller 10 is provided on the front side of the blower 9, and a control panel 4 is mounted on the front surface of the base 3. All electrical components are connected to the main controller 10 through wires, and the main controller 10 is electrically connected to the control panel 4 through wires.

[0042] In this embodiment, an adjustable exhaust port 5 is installed on the top of the insulated box 1 to maintain the stability of the pressure inside the box and ensure the normal operation of the device.

[0043] The working principle of this blood cell circulation culture device:

[0044] In practical use, when blood cells need to be cultured, first connect one end of the circulating tubing 73 to the lower end of the spiral culture tube 711 via the sealing connector 79. Then close the tubing valve 78 on the circulating tubing 73 and add the culture medium containing blood cells into the spiral culture tube 711 through the upper port. Next, connect the other end of the circulating tubing 73 to the upper port of the spiral culture tube 711 via the sealing connector 79. Then open the incubator door 2, clamp the protective sleeve 71 onto the support 72 to fix the protective sleeve 71, and then wrap the right half of the circulating tubing 73 around the peristaltic pump 74 and open the tubing valve 78. The operating parameters of the peristaltic pump 74, blower 9, and constant-temperature electric heater 13 are adjusted via control panel 4. Then, blower 9 and constant-temperature electric heater 13 are started, setting the heating temperature of constant-temperature electric heater 13 to 37 degrees Celsius. The constant-temperature electric heater 13 generates heat, which is then blown by blower 9 through air outlet 8 into the insulation chamber 1 to heat the culture medium, maintaining the internal temperature of the insulation chamber 1 at 37 degrees Celsius. Next, peristaltic pump 74 is started, driving the culture medium to circulate in the spiral culture tube 711 and circulating tubing 73, simulating the blood flow environment in the human body.

[0045] Once the culture time has reached the preset value or the cell culture has achieved the expected results, press the stop button on control panel 4 to turn off all electrical components. Open the incubator door 2, detach the culture container from the circulation tubing 73, and remove the cultured blood cells for subsequent experiments or analysis.

[0046] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating this utility model, and are not intended to limit the implementation of this utility model. For those skilled in the art, other variations or modifications can be made based on the above description. It is impossible to exhaustively list all the implementation methods here. Any obvious variations or modifications derived from the technical solutions of this utility model are still within the protection scope of this utility model.

Claims

1. A blood cell circulation culture device, characterized in that: It includes an insulated box (1) and a base (3), with the insulated box (1) installed on the upper side of the base (3), and a circulating culture mechanism (7) provided inside the insulated box (1). The circulating culture mechanism (7) includes a protective sleeve (71), a circulating hose (73), a peristaltic pump (74), and a spiral culture tube (711). The protective sleeve (71) is located inside the heat preservation box (1). A transparent spiral culture tube (711) is provided inside the protective sleeve (71), and the upper and lower ends of the spiral culture tube (711) extend from the upper and lower ends of the protective sleeve (71). The upper and lower ends of the spiral culture tube (711) are connected to the transparent circulating hose (73) through a sealing connector (79). The peristaltic pump (74) is installed on the right side of the heat preservation box (1) through a fixing frame (75). The right section of the circulating hose (73) is detachably wrapped inside the peristaltic pump (74).

2. The blood cell circulation culture device according to claim 1, characterized in that: The heat preservation box (1) has two sets of brackets (72) on the inner left side wall, and the protective sleeve (71) is detachably clamped on the two sets of brackets (72); The bracket (72) consists of a fixed support rod (721) and a U-shaped hanger (722). One end of the fixed support rod (721) is fixedly connected to the inner wall of the insulation box (1), and the other end is provided with a U-shaped hanger (722). The specifications of the U-shaped hanger (722) are compatible with the specifications of the protective tube (71). Two sets of positioning baffles (76) are provided on the ring surface of the protective tube (71), and the diameter of the positioning baffles (76) is the same as the diameter of the U-shaped hanger (722).

3. The blood cell circulation culture device according to claim 1, characterized in that: A hose valve (78) is installed near the upper end of the circulation hose (73).

4. The blood cell circulation culture device according to claim 3, characterized in that: The spiral culture tube (711) has an air inlet (710) on its upper ring surface that is connected to its interior, and the upper end of the air inlet (710) is inclined outward.

5. The blood cell circulation culture device according to claim 4, characterized in that: The protective sleeve (71) is made of medical transparent plastic, and multiple sets of through grooves (77) are evenly opened on its annular surface.

6. The blood cell circulation culture device according to claim 1, characterized in that: The insulated box (1) has an openable insulated box door (2) installed on the front side.

7. The blood cell circulation culture device according to claim 1, characterized in that: The base (3) is provided with a partition (11) inside. A constant temperature electric heater (13) is installed in the right area of ​​the partition (11). A blower (9) is installed in the left area of ​​the partition (11). A ventilation slot (12) is opened on the partition (11). The blower (9) is connected to the ventilation slot (12). A heat dissipation vent (6) is opened on the left side of the base (3) corresponding to the blower (9). Multiple sets of air outlets (8) are evenly opened on the upper surface of the base (3).

8. The blood cell circulation culture device according to claim 7, characterized in that: The blower (9) is equipped with a main controller (10) on the front side, and a control panel (4) is mounted on the front surface of the base (3). All electrical components are connected to the main controller (10) through wires, and the main controller (10) is electrically connected to the control panel (4) through wires.

9. The blood cell circulation culture device according to claim 1, characterized in that: An adjustable exhaust port (5) is installed on the top of the insulated box (1).

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