Rapid identification device for animal-derived components

Abstract

The utility model relates to a kind of animal-derived component rapid identification device, belong to detection equipment field.The reagent reserve tank bottom is connected with material guide pipe, a first liquid outlet pipe is docked in bifurcation on the material guide pipe, the first liquid outlet pipe is equipped with first liquid outlet control valve, quantitative identification module is provided at the first liquid outlet pipe end, component detection mechanism is provided in the material guide pipe directly below, the component detection mechanism includes stable pedestal, heating incubator is fixed in the stable pedestal top, component detection groove is provided in the heating incubator, a temperature control space is formed between component detection groove and heating incubator, electric heating toroidal pipe is installed in the temperature control space inner wall.The utility model can realize the incubation temperature regulation when the animal-derived component sample to be identified and known antibody binding reaction, improve the sensitivity of animal-derived component enzyme label detection, guarantee the accuracy of identification result.

Description

Technical Field

[0001] This utility model relates to the field of detection equipment technology, and in particular to a rapid identification device for animal-derived components. Background Technology

[0002] With the increasing demand for animal-derived component detection in fields such as food safety, biomedicine, import and export inspection and quarantine, and environmental protection, the rapid and accurate identification of animal-derived components has become a focus of research and application. Currently, the commonly used method for identifying animal-derived components is enzyme-linked immunosorbent assay (ELISA), which has high sensitivity and specificity and is widely used for sample identification under laboratory conditions. However, current ELISA identification of animal-derived components typically requires operators to manually add sample solutions and enzyme-labeled secondary antibody substrate solutions step by step, which is cumbersome and consumes a lot of manpower and time, while also making it difficult to guarantee the accuracy of quantitative addition. Furthermore, the binding reaction between animal-derived components and known antibodies requires a suitable temperature environment, but existing ELISA assays lack corresponding incubation equipment and regulation functions, posing certain limitations and making the identification of animal-derived components by ELISA prone to inaccuracies, thus reducing the accuracy and reliability of the identification results. Utility Model Content

[0003] This invention overcomes the shortcomings of the prior art and provides a rapid identification device for animal-derived components.

[0004] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0005] This invention provides a rapid identification device for animal-derived components, which includes a reagent storage box:

[0006] The reagent storage tank is connected to a feed tube at the bottom. A first liquid outlet conduit is forked and connected to the feed tube. The first liquid outlet conduit is equipped with a first liquid outlet control valve. A quantitative identification module is set at the end of the first liquid outlet conduit. A component detection mechanism is set directly below the feed tube. The component detection mechanism includes a stable base. A heating incubator is fixed on the top of the stable base. A component detection tank is set inside the heating incubator. A temperature-controlled space is formed between the component detection tank and the heating incubator. An electric heating ring is installed on the inner wall of the temperature-controlled space.

[0007] Furthermore, in a preferred embodiment of the present invention, an annular guide groove is provided between the top of the heating incubator and the component detection groove, and the annular guide groove is used to install the temperature control barrier ring plate.

[0008] Furthermore, in a preferred embodiment of the present invention, a guide plate is welded above the temperature control barrier ring plate, and the guide plate and the temperature control barrier ring plate are T-shaped.

[0009] Furthermore, in a preferred embodiment of the present invention, the guide plate has two symmetrically distributed guide holes, each guide hole through which a guide rod passes, and the two guide rods are disposed on the top of the heating incubator.

[0010] Furthermore, in a preferred embodiment of the present invention, a lead screw nut is provided on the guide plate, and the lead screw nut is threadedly engaged with the lead screw.

[0011] Furthermore, in a preferred embodiment of the present invention, the lead screw is mounted on an arc-shaped bracket, the arc-shaped bracket is disposed on the side of the heating incubator, and the end of the lead screw is connected to a servo motor.

[0012] Furthermore, in a preferred embodiment of the present invention, the component detection tank is connected to a second liquid outlet conduit, and the second liquid outlet conduit is equipped with a second liquid outlet control valve.

[0013] Furthermore, in a preferred embodiment of the present invention, the end of the second liquid outlet conduit is provided with the same quantitative identification module.

[0014] Furthermore, in a preferred embodiment of the present invention, the quantitative identification module includes a quantitative storage box, and an identification dropper is provided at the bottom of the quantitative storage box.

[0015] Furthermore, in a preferred embodiment of the present invention, an I-shaped piston rod is disposed above the identification dropper, the I-shaped piston rod passes through the top of the quantitative liquid storage box, and a return spring is installed between the top surface of the quantitative liquid storage box and the top end of the I-shaped piston rod.

[0016] The beneficial technical effects of this utility model are as follows:

[0017] This invention involves adding the animal-derived component sample to be identified along with a known antibody into a component detection cell for binding reaction, and controlling an electrically heated loop tube to incubate the sample. If the temperature is not reached, a servo motor, driven by a lead screw, rotates and guides a temperature-controlled barrier plate upwards. This gradually removes the barrier between the electrically heated loop tube and the component detection cell, allowing most of the heat generated by the loop tube to flow towards the outer wall of the component detection cell, thereby increasing the internal incubation temperature. The degree of raising and lowering of the temperature-controlled barrier plate is controllable, achieving temperature regulation and effectively reducing the incidence of non-specific binding. This improves the sensitivity of subsequent enzyme-labeled detection of animal-derived components, ensuring the accuracy and reliability of the identification results. Attached Figure Description

[0018] The accompanying drawings, which form part of this specification, are used to provide a further understanding of this utility model. The illustrative embodiments of this utility model and their descriptions are used to explain this utility model and do not constitute an improper limitation of this utility model.

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a partial structural schematic diagram of the present invention;

[0021] Figure 3 This is a cross-sectional structural diagram of the present invention.

[0022] In the picture:

[0023] 1. Reagent storage box; 2. Feed tube; 3. First liquid outlet tube; 4. First liquid outlet control valve; 5. Stabilizing base; 6. Heating incubator; 7. Component detection tank; 8. Electric heating ring tube; 9. Temperature control barrier ring plate; 10. Guide plate; 11. Guide rod; 12. Screw nut; 13. Screw; 14. Bow-shaped support; 15. Servo motor; 16. Second liquid outlet tube; 17. Second liquid outlet control valve; 18. Quantitative liquid storage box; 19. Identification dropper; 20. I-shaped piston rod; 21. Return spring; 22. Colorimetric slide. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] In the description of this utility model, references to "embodiment," "one embodiment," "some embodiments," or "other embodiments" indicate that a specific feature, structure, or characteristic described in connection with an embodiment is included in at least some embodiments, but not necessarily all embodiments. Multiple appearances of "embodiment," "one embodiment," or "some embodiments" do not necessarily refer to the same embodiment. If the specification describes a component, feature, structure, or characteristic as "may," "may," or "can" be included, then that particular component, feature, structure, or characteristic is not required to be included. If the specification or claims refer to an element "a," it does not mean that there is only one element. If the specification or claims refer to "an additional" element, it does not exclude the existence of more than one additional element. Furthermore, specific features, structures, functions, or characteristics can be combined in one or more embodiments in any suitable manner. For example, a first embodiment can be combined with a second embodiment, provided that the specific features, structures, functions, or characteristics associated with the two embodiments are not mutually exclusive.

[0026] In the description of this utility model, unless otherwise specified, ordinal adjectives such as "first," "second," and "third" are used to describe common objects, indicating only different instances of the same object, and not implying that the objects described must be in a given order, whether temporally, spatially, sequentially, or in any other way. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0027] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0028] Example

[0029] like Figure 1-3 As shown, this application provides a rapid identification device for animal-derived components, which includes a reagent storage box 1.

[0030] The reagent storage tank 1 is connected to a feed tube 2 at the bottom. A first liquid outlet conduit 3 is forked and connected to the feed tube 2. A first liquid outlet control valve 4 is installed on the first liquid outlet conduit 3. A quantitative identification module is set at the end of the first liquid outlet conduit 3. A component detection mechanism is set directly below the feed tube 2. The component detection mechanism includes a stable base 5. A heating incubator 6 is fixed on the top of the stable base 5. A component detection tank 7 is set inside the heating incubator 6. A temperature control space is formed between the component detection tank 7 and the heating incubator 6. An electric heating ring tube 8 is installed on the inner wall of the temperature control space.

[0031] Furthermore, in a preferred embodiment of the present invention, an annular guide groove is provided between the top of the heating incubator 6 and the component detection groove 7, and the annular guide groove is used to install the temperature control barrier ring plate 9.

[0032] Furthermore, in a preferred embodiment of the present invention, a guide plate 10 is welded above the temperature control barrier ring plate 9, and the guide plate 10 and the temperature control barrier ring plate 9 are T-shaped.

[0033] Furthermore, in a preferred embodiment of the present invention, the guide plate 10 has two symmetrically distributed guide holes, each guide hole passing through a guide rod 11, and the two guide rods 11 are disposed on the top of the heating incubator 6.

[0034] Furthermore, in a preferred embodiment of the present invention, a lead screw nut 12 is provided on the guide plate 10, and the lead screw nut 12 is threadedly engaged with the lead screw 13.

[0035] Furthermore, in a preferred embodiment of the present invention, the lead screw 13 is mounted on the bow-shaped bracket 14, the bow-shaped bracket 14 is disposed on the side of the heating incubator 6, and the end of the lead screw 13 is connected to the servo motor 15.

[0036] Furthermore, in a preferred embodiment of the present invention, the component detection tank 7 is connected to a second liquid outlet conduit 16, and the second liquid outlet conduit 16 is equipped with a second liquid outlet control valve 17.

[0037] Furthermore, in a preferred embodiment of the present invention, the second liquid outlet conduit 16 is provided with the same quantitative identification module at its end.

[0038] Furthermore, in a preferred embodiment of the present invention, the quantitative identification module includes a quantitative storage box 18, and an identification drop tube 19 is provided at the bottom of the quantitative storage box 18.

[0039] Furthermore, in a preferred embodiment of the present invention, an I-shaped piston rod 20 is disposed above the identification drop tube 19, the I-shaped piston rod 20 passes through the top of the quantitative liquid storage box 18, and a return spring 21 is installed between the top surface of the quantitative liquid storage box 18 and the top end of the I-shaped piston rod 20.

[0040] The working process of this utility model is as follows:

[0041] The reagent storage tank 1 is used to store an appropriate amount of enzyme-labeled secondary antibody substrate reagent. Known antibodies from other animal sources are extracted. The animal-derived component sample to be identified, along with the extracted known antibody, is added to the component detection tank 7. At this time, the electric heating loop 8 is activated. As the energizing time increases, the temperature inside the temperature-controlled space continuously rises, allowing heat to be transferred to the component detection tank 7 through the temperature-controlled barrier ring 9. This achieves the incubation and binding reaction between the animal-derived component sample to be identified and the known antibody. If the incubation temperature is lower than the target temperature, the servo motor 15 is activated during the continuous heating process of the electric heating loop 8. The output shaft of the servo motor 15 drives the lead screw 13 to rotate on the bow-shaped support 14. Under the threaded engagement, the rotation of the lead screw 13 drives the lead screw nut 12 along the lead screw 13. The upward movement of the temperature-controlled barrier ring plate 9, guided by the guide plate 10, causes the temperature-controlled barrier ring plate 9 to rise simultaneously. As the temperature-controlled barrier ring plate 9 rises, the obstruction between the electric heating ring tube 8 and the component detection tank 7 is gradually removed. At this time, a large amount of heat from the electric heating ring tube 8 will flow to the outer wall of the component detection tank 7 in the temperature-controlled space, thereby further increasing the internal incubation temperature of the component detection tank 7. The increase in incubation temperature can be adjusted by controlling the degree of raising and lowering of the temperature-controlled barrier ring plate 9 by the servo motor 15, thus achieving the effect of temperature regulation. This creates a suitable temperature environment for the animal-derived component sample to be identified and the known antibody to recognize and bind to each other in a highly specific manner, reducing the probability of non-specific binding, effectively improving the sensitivity of subsequent enzyme labeling detection, and ensuring the accuracy and reliability of the identification results.

[0042] After the incubation reaction is complete, rotate the first outlet control valve 4 to open the first outlet conduit 3, allowing the secondary antibody substrate reagent stored in the reagent storage tank to be added through the feed tube 2 to the quantitative storage box 18 connected to the end of the first outlet conduit 3. At this time, pull the I-shaped piston rod 20 of the quantitative storage box 18 upward, and the piston at the bottom of the I-shaped piston rod 20 will be lifted upward, thereby opening the outlet of the identification drop tube 19 blocking the bottom of the quantitative storage box 18, so that the secondary antibody substrate reagent is quantitatively dripped onto the chromogenic slide 22 directly below. After the quantitative addition is completed, remove the force of pulling the I-shaped piston rod 20 upward. Under the action of the return spring 21, the I-shaped piston rod 20 returns to its original position downward, and the piston at the bottom re-blocks the outlet of the identification drop tube 19, completing the addition step of the enzyme-catalyzed chromogenic substrate. At this point, the chromogenic slide 22, with the secondary antibody substrate reagent added, is moved to the area below the quantitative identification module connected to the second outlet conduit 16. The second outlet control valve 17 is rotated, allowing the binding solution of the animal-derived component to be identified, after the incubation and binding reaction is complete, to enter the connected quantitative storage box 18 along the second outlet conduit 16. Similarly, the I-shaped piston rod 20 on the quantitative storage box 18 is pulled upwards, causing the binding solution to fall onto the chromogenic slide 22 through the opened identification dropper 19. The binding solution then reacts with the pre-added secondary antibody substrate reagent. If the corresponding antigen is present in the animal-derived component to be identified, it will bind to the known antibody, and a response color will appear through the secondary antibody substrate reagent. This allows for rapid determination of the presence of a specific animal-derived component in the sample, achieving rapid identification of animal-derived components. This device can replace the cumbersome steps of traditional manual identification, significantly improving the identification speed, ensuring the accuracy and reliability of the final identification results, and simultaneously meeting the requirements for quantitative and controllable identification operations, avoiding component identification errors and resource waste.

[0043] It should be noted that the temperature control barrier ring plate is a metal matrix composite material made of aluminum plate as the base and ceramic particles added. It combines the good processing performance of aluminum with the high thermal conductivity of ceramic particles. When necessary, its thermal conductivity can reach 180-220 W / (m·K). It has good thermal conductivity and high temperature resistance, which can make the heat transfer from the electric heating ring tube to the component detection tank more uniform and reasonable, and ensure the accuracy and stability of the incubation temperature.

[0044] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of this technical solution, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A rapid identification device for animal-derived components, the rapid identification device for animal-derived components comprising a reagent storage tank, characterized in that: The reagent storage tank is connected to a feed tube at the bottom. A first liquid outlet conduit is forked and connected to the feed tube. The first liquid outlet conduit is equipped with a first liquid outlet control valve. A quantitative identification module is set at the end of the first liquid outlet conduit. A component detection mechanism is set directly below the feed tube. The component detection mechanism includes a stable base. A heating incubator is fixed on the top of the stable base. A component detection tank is set inside the heating incubator. A temperature-controlled space is formed between the component detection tank and the heating incubator. An electric heating ring is installed on the inner wall of the temperature-controlled space.

2. The animal-derived component rapid identification device according to claim 1, characterized by A circular guide groove is provided between the top of the heating incubator and the component detection tank, and the circular guide groove is used to install the temperature control barrier ring plate.

3. The animal-derived component rapid identification device according to claim 2, characterized by A guide plate is welded above the temperature control barrier ring plate, and the guide plate and the temperature control barrier ring plate are T-shaped.

4. The animal-derived component rapid identification device according to claim 3, wherein The guide plate has two symmetrically distributed guide holes, each guide hole through which a guide rod passes, and the two guide rods are located at the top of the heating incubator.

5. The animal-derived component rapid identification device according to claim 4, wherein The guide plate is provided with a lead screw nut, which is threadedly engaged with the lead screw.

6. The animal-derived component rapid identification device according to claim 5, wherein The lead screw is mounted on an arc-shaped bracket, which is located on the side of the heating incubator, and the end of the lead screw is connected to a servo motor.

7. The animal-derived component rapid identification device according to claim 1, wherein The component detection tank is connected to a second liquid outlet conduit, and the second liquid outlet conduit is equipped with a second liquid outlet control valve.

8. The animal-derived component rapid identification device according to claim 7, characterized by The second liquid outlet conduit is equipped with the same quantitative identification module at its end.

9. The animal-derived component rapid identification device according to claim 1, wherein The quantitative identification module includes a quantitative liquid storage box, and an identification drop tube is provided at the bottom of the quantitative liquid storage box.

10. The animal-derived component rapid identification device according to claim 9, wherein An I-shaped piston rod is positioned above the identification dropper, and the I-shaped piston rod extends through the top of the quantitative liquid storage box. A return spring is installed between the top surface of the quantitative liquid storage box and the top of the I-shaped piston rod.

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