An apparatus for the isolation and collection of mouse metabolites

Abstract

The utility model relates to a mouse metabolite's separation and collection device belongs to mouse metabolite separation and collection technical field. The mouse metabolite's separation and collection device, include: the mount pad, the top fixedly connected with the transparent frame of mount pad, the separation mechanism includes fixedly connected in the mount pad top's openwork board, the inner wall of mount pad is clamped to have the filter plate, the bottom fixedly connected with the urine guide board of filter plate, the surface of urine guide board is " V " shape, through openwork board, filter plate and urine guide board mutual cooperation, realized to mouse excretion's urine and excrement and carried out separation, and then avoided urine and excrement mixing, thereby avoided the cross -contamination situation of metabolite sample, guaranteed the accurate analysis to single metabolite, through the filter plate and urine guide board of the angle that the bottom formed with horizontal surface, the excrement and urine of convenient separation after quick slide into sample collection box, reduce the probability that metabolite stays on filter plate and urine guide board.

Description

Technical Field

[0001] This utility model relates to the field of mouse metabolite separation and collection technology, and in particular to a device for separating and collecting mouse metabolites. Background Technology

[0002] Mice have become one of the most widely used model animals in biomedical research due to their high similarity to the human genome, ease of breeding and raising, and clear genetic background. Therefore, metabolic cages are used to collect mouse metabolites in the rearing of mice. Analyzing mouse urine, feces and other metabolites is a key means of assessing their physiological state, disease progression, drug efficacy and toxicity, nutritional metabolism and the impact of environmental exposure.

[0003] In medical research, most existing university research laboratories place mice in metabolic cages with a grid bottom and a tray underneath to collect excrement. This can easily lead to the mixing of urine and feces, resulting in cross-contamination of metabolite samples and affecting the accurate analysis of individual metabolites. Utility Model Content

[0004] Therefore, it is necessary to provide a device for separating and collecting mouse metabolites, which addresses the problem that mice are often placed in metabolic cages with a mesh bottom and a tray underneath to collect excrement, easily leading to the mixing of urine and feces.

[0005] It includes: a mounting base, the top of which is fixedly connected to a transparent frame; a separation mechanism, the separation mechanism including a perforated plate fixedly connected to the top of the mounting base, a filter plate snapped into the inner wall of the mounting base, a catheter plate fixedly connected to the bottom of the filter plate, the surface of the catheter plate being "V" shaped, and the bottom of both the filter plate and the catheter plate forming an angle with the horizontal plane.

[0006] In one embodiment, a water-fixing frame is installed on the top of the mounting base. The positioning frame positions the mouse-specific water bottle so that the water outlet of the water bottle extends through and into the transparent frame, thereby facilitating watering of the mouse inside the transparent frame and enabling the mouse to excrete urine.

[0007] In one embodiment, a blocking plate is fixedly connected to the top of the mounting base, the bottom of the blocking plate forms an angle with the horizontal plane, and the top of the blocking plate is curved into an arc shape.

[0008] In one embodiment, a transparent panel is hinged to the top of the transparent frame.

[0009] In one embodiment, the inner wall of the mounting base is slidably connected with two positioning frames, the surfaces of which are engaged with the inner wall of the filter plate.

[0010] In one embodiment, guide rods are fixedly connected to both the front and rear ends of the mounting base, and the inner wall of the positioning frame is slidably connected to the surface of the guide rods. The cooperation between the guide rods and the positioning frame allows for the disassembly of the filter plate and catheter plate on the mounting base, facilitating cleaning and ensuring their cleanliness, thereby preventing cross-infection during metabolite collection.

[0011] In one embodiment, a spring is fixedly connected to one end of the guide rod, and the other end of the spring is fixedly connected to the end of the positioning frame.

[0012] In one embodiment, a long rod is slidably connected to the upper inner wall of the transparent frame, and the surface of the long rod is engaged with the inner wall of the transparent plate. The interaction between the long rod and the transparent plate effectively seals the opening at the top of the transparent frame, preventing the mouse from escaping and thus ensuring the effective collection of mouse metabolites.

[0013] Beneficial effects

[0014] 1. By using a perforated plate, filter plate, and urine guide plate in combination, the urine and feces excreted by mice can be separated, thus avoiding the mixing of urine and feces and preventing cross-contamination of metabolite samples. This ensures accurate analysis of a single metabolite. The filter plate and urine guide plate, which form an angle between their bottom and the horizontal plane, allow the separated feces and urine to slide quickly into the sample collection box, reducing the probability of metabolites remaining on the filter plate and urine guide plate.

[0015] 2. The interceptor plate is used to prevent water leaking from the drinking bottle from sliding down the perforated plate and filter plate onto the urine guide plate, thus avoiding mixing of drinking water and collected urine, ensuring the accuracy of mouse urine collection, and thus ensuring accurate urine analysis. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the separation mechanism of this utility model;

[0019] Figure 3 This utility model Figure 2 Enlarged view of point A in the middle;

[0020] Figure 4 This utility model Figure 2 Enlarged view of point B in the middle;

[0021] Figure 5 This is an exploded view of the filter plate and urine guide plate of this utility model.

[0022] Figure label:

[0023] 100. Mounting base; 200. Transparent frame; 300. Separation mechanism; 301. Filter plate; 302. Urine guide plate; 303. Water-fixing frame; 304. Interception plate; 305. Transparent plate; 306. Positioning frame; 307. Guide rod; 308. Spring; 309. Hollow plate; 310. Long rod. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0025] The following is combined Figures 1-5 This invention describes a device for separating and collecting mouse metabolites.

[0026] In one embodiment, a mouse metabolite separation and collection device includes: a mounting base 100, with a transparent frame 200 fixedly connected to the top of the mounting base 100; a separation mechanism 300, including a perforated plate 309 fixedly connected to the top of the mounting base 100, a filter plate 301 snapped into the inner wall of the mounting base 100, and a urine catheter plate 302 fixedly connected to the bottom of the filter plate 301, the surface of the urine catheter plate 302 being "V" shaped, and the bottoms of both the filter plate 301 and the urine catheter plate 302 forming an angle with the horizontal plane.

[0027] It should be noted that the filter plate 301 is made of nylon, which is soft and has a certain degree of elasticity. It can better conform to the shape of the collected feces, reduce gaps, and prevent feces from leaking onto the urine guide plate 302.

[0028] It can effectively separate mouse feces and urine, meeting the experimental requirements for filtration accuracy.

[0029] Both the catheter plate 302 and the perforated plate 309 are made of stainless steel, which is not easily damaged and can withstand certain external impacts and mouse activity, resulting in a long service life.

[0030] Its smooth surface makes it difficult for bacteria to grow, and it can be thoroughly disinfected through various methods such as high temperature and chemical disinfectants to ensure hygienic experimental conditions.

[0031] The stainless steel urinary guide plate 302 and the perforated plate 309 can resist the corrosion of urine and maintain the stable performance of the urinary guide plate 302 and the perforated plate 309.

[0032] In this embodiment, when students in the high-efficiency scientific research laboratory need to collect mouse metabolites, a urine collection box is placed below the urine catheterization plate 302, and a feces collection box is placed below the filter plate 301. The mice are placed in a transparent frame 200 for rearing. When the mice excrete metabolites, the aperture of the perforated plate 309 is larger than the excreted feces and urine, causing the feces and urine to fall onto the filter plate 301. The filter plate 301 is used to prevent feces from falling onto the urine catheterization plate 302. Urine flows through the filter holes on the filter plate 301 and falls onto the urine catheterization plate 302. Because the surface of the urine catheterization plate 302 is "V" shaped and the bottom forms an angle with the horizontal plane, the urine is quickly guided into the urine collection box. Because the bottom of the filter plate 301 forms an angle with the horizontal plane, the feces falling on the top of the filter plate 301 slide into the feces collection box.

[0033] After collection, the students can conduct relevant studies on the collected fecal and urine samples. The procedures for studying fecal and urine samples are existing well-known techniques and will not be elaborated here.

[0034] like Figure 2 As shown, a water-fixing frame 303 is installed on the top of the mounting base 100, and an interceptor plate 304 is fixedly connected to the top of the mounting base 100. The bottom of the interceptor plate 304 forms an angle with the horizontal plane, and the top of the interceptor plate 304 is curved into an arc shape.

[0035] It should be noted that a screw is fixedly connected to the bottom of the water-fixing frame 303, and a nut is threaded onto the surface of the screw. The water-fixing frame 303 is installed on the top of the mounting base 100 by means of the screw and the nut.

[0036] In this embodiment, a water bottle specifically for mice is placed inside the water-fixing frame 303, and the water outlet at one end of the water bottle is inserted into a pre-reserved round hole on one side of the transparent frame 200, so that the mice inside the transparent frame 200 can drink water at any time, making it convenient for the mice to urinate.

[0037] Such as 2 and Figure 4 As shown, a transparent plate 305 is hinged to the top of the transparent frame 200, and a long rod 310 is slidably connected to the upper end of the inner wall of the transparent frame 200. The surface of the long rod 310 is engaged with the inner wall of the transparent plate 305.

[0038] Both the transparent frame 200 and the transparent plate 305 are acrylic components, which have extremely high transparency and excellent optical stability, allowing researchers to clearly observe the mice excreting metabolites and their activities within the transparent frame 200.

[0039] It can maintain good optical performance during long-term use and is not prone to yellowing or discoloration.

[0040] In this embodiment, the long rod 310 is pulled away from the transparent plate 305, and the transparent plate 305 is pulled to open the top of the transparent frame 200, so that the mouse can be placed in the transparent frame 200. The transparent plate 305 is pushed to flip and touch the transparent frame 200. The long rod 310 is slid into the transparent frame 200 and the transparent plate 305, so that the transparent plate 305 is stably limited in the transparent frame 200.

[0041] like Figure 3 As shown, two positioning frames 306 are slidably connected to the inner wall of the mounting base 100. The surface of the positioning frame 306 is snapped onto the inner wall of the filter plate 301. Guide rods 307 are fixedly connected to both the front and rear ends of the mounting base 100. The inner wall of the positioning frame 306 is slidably connected to the surface of the guide rod 307. A spring 308 is fixedly connected to the end of the guide rod 307. The other end of the spring 308 is fixedly connected to the end of the positioning frame 306.

[0042] In this embodiment, when the filter plate 301 and the catheter plate 302 need to be cleaned, the positioning brackets 306 at both ends are pulled away from the filter plate 301 and the filter plate 301 is pulled away from the mounting base 100, so that the filter plate 301 and the catheter plate 302 can be cleaned.

[0043] After the filter plate 301 has been cleaned and dried, it is secured in the mounting base 100. The positioning frame 306 is loosened, and the elastic force of the spring 308 pushes the positioning frame 306 to move and secure it in the filter plate 301, so that the filter plate 301 is installed on the mounting base 100.

[0044] Working principle: Pull the long rod 310 away from the transparent plate 305, pull the transparent plate 305, and open the top of the transparent frame 200. Then, the mouse can be placed in the transparent frame 200. When the mouse excretes metabolites, because the aperture of the perforated plate 309 is larger than the excreted feces and urine, the feces and urine fall onto the filter plate 301. The filter plate 301 is used to prevent feces from falling onto the urine guide plate 302. Because the bottom of the filter plate 301 forms an angle with the horizontal plane, the feces falling on the top of the filter plate 301 slide into the feces collection box. The urine flows through the filter holes on the filter plate 301 into the urine guide plate 302. Because the surface of the urine guide plate 302 is "V" shaped and the bottom forms an angle with the horizontal plane, the urine is quickly guided into the urine collection box.

[0045] It should be noted that the mounting base 100, transparent frame 200, transparent plate 305, water-fixing frame 303 and hollow plate 309 mentioned above are all devices with relatively mature existing technology. The specific models can be selected according to actual needs, and will not be elaborated here.

[0046] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A device for separating and collecting mouse metabolites, characterized in that, include: Mounting base (100), the top of which is fixedly connected to a transparent frame (200); The separation mechanism (300) includes a perforated plate (309) fixedly connected to the top of the mounting base (100). A filter plate (301) is snapped into the inner wall of the mounting base (100). A urine catheter plate (302) is fixedly connected to the bottom of the filter plate (301). The surface of the urine catheter plate (302) is "V" shaped. The bottom of both the filter plate (301) and the urine catheter plate (302) form an angle with the horizontal plane.

2. The device for separating and collecting mouse metabolites according to claim 1, characterized in that, A water-fixing frame (303) is installed on the top of the mounting base (100).

3. The device for separating and collecting mouse metabolites according to claim 1, characterized in that, An interceptor plate (304) is fixedly connected to the top of the mounting base (100). The bottom of the interceptor plate (304) forms an angle with the horizontal plane, and the top of the interceptor plate (304) is curved into an arc shape.

4. The device for separating and collecting mouse metabolites according to claim 1, characterized in that, A transparent plate (305) is hinged to the top of the transparent frame (200).

5. The device for separating and collecting mouse metabolites according to claim 1, characterized in that, The inner wall of the mounting base (100) is slidably connected to two positioning frames (306), and the surface of the positioning frame (306) is engaged with the inner wall of the filter plate (301).

6. The mouse metabolite separation and collection device according to claim 5, characterized in that, Guide rods (307) are fixedly connected to both the front and rear ends of the mounting base (100), and the inner wall of the positioning frame (306) is slidably connected to the surface of the guide rods (307).

7. The apparatus for separating and collecting mouse metabolites according to claim 6, characterized in that, A spring (308) is fixedly connected to the end of the guide rod (307), and the other end of the spring (308) is fixedly connected to the end of the positioning frame (306).

8. The device for separating and collecting mouse metabolites according to claim 1, characterized in that, A long rod (310) is slidably connected to the upper end of the inner wall of the transparent frame (200), and the surface of the long rod (310) is engaged with the inner wall of the transparent plate (305).

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