A platelet-rich plasma preparation kit

Abstract

The utility model relates to medical instrument technical field discloses a kind of platelet-rich plasma preparation kits, including centrifugal tube, liquid guide connector;Centrifugal tube body front upper portion is equipped with the luer taper head of the elastic valve body including slit;Luer taper head includes taper head shell, taper head base, taper head base is connected with centrifugal tube body, taper head shell inside and taper head base form cavity, protruding pipeline is equipped in the middle of taper head base front end, elastic valve body is wrapped in taper head shell, elastic valve body is hollow, and wrapping protruding pipeline;Liquid guide connector is the luer external conical locking connector with rotatable connection's internal thread collar, and the catheter with external conical luer connector is internally arranged, and catheter can be inserted into luer taper head with protruding pipeline communication by the slit of elastic valve body. In the utility model, liquid guide connector and luer taper head are communicated in elastic valve body and form airtight passage, and elastic valve body can close quickly when separating liquid guide connector, effectively prevent the risk of air bacteria to blood pollution.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to a platelet-rich plasma preparation kit. Background Technology

[0002] Platelet-rich plasma (PRP), rich in growth factors and platelets, is widely used in tissue repair, wound healing, and cosmetic applications. Traditional PRP preparation typically involves multiple steps, including blood collection, centrifugation, and plasma extraction. Blood needs to be transferred between multiple containers and connecting devices, many of which are open designs exposed to air, increasing the risk of contamination. For example, peripheral venous blood is usually collected using syringes and then transferred to centrifuge containers, and the conical tip of a typical syringe has an open structure, posing a risk of blood leakage after collection.

[0003] Current PRP preparation technologies mostly employ a two-stage centrifugation method. After the first centrifugation, the blood is separated into a plasma layer, a white membrane layer, and a red blood cell layer. The red blood cell layer is discarded after the first centrifugation, and the white membrane layer and plasma layer are collected. After the second centrifugation, the excess plasma is extracted, and the remaining portion is PRP. However, when collecting the plasma layer and white membrane layer after the first centrifugation, the white membrane layer is relatively thin and comes into direct contact with the red blood cell layer. Current technologies are not precise enough in extracting the white membrane layer, which can easily lead to incomplete collection of the white membrane layer or excessive red blood cell contamination. Furthermore, it requires the coordinated use of various medical consumables, which can easily contaminate the extracted PRP. The process is complex and inefficient. Utility Model Content

[0004] To address the shortcomings of the existing technology, this invention provides a platelet-rich plasma preparation kit, which reduces the risk of exposure during blood transfer in the platelet-rich plasma preparation process, and features simple preparation operation and high purity of the prepared platelet-rich plasma.

[0005] To achieve the above-mentioned technical objectives, the technical solution adopted by this utility model is as follows:

[0006] This utility model provides a platelet-rich plasma preparation kit, including: centrifuge tubes and a liquid delivery connector;

[0007] The centrifuge tube has a Luer cone with a slit-shaped elastic valve body at the upper front end. The Luer cone includes a cone shell and a cone base. The cone base is connected to the centrifuge tube and the cone shell. The inner side of the cone shell and the cone base form a cavity. A protruding tube is provided in the middle of the front end of the cone base. The elastic valve body is enclosed in the cone shell. The outer surface of the elastic valve body is adapted to the shape of the inner surface of the cone shell. The elastic valve body is hollow inside and encloses the protruding tube. The end of the cone shell away from the cone base has an external thread. The inner wall of the centrifuge tube is sealed and slidably connected to a sealing elastic piston. The sealing elastic piston is detachably connected to a core rod.

[0008] The fluid guide connector is a Luer external cone locking connector equipped with a rotatable internal thread retaining ring. It has a conduit with an external cone Luer connector inside. The conduit can be inserted into the Luer cone head through the slit of the elastic valve body and communicate with the protruding pipeline. The retaining ring of the fluid guide connector is connected to the cone head shell. The fluid guide connector has a symmetrical double-head structure.

[0009] As a preferred technical solution, the cone base and the centrifuge tube body are integrally molded from polymer material.

[0010] As a preferred technical solution, the cone base is made of polypropylene, Teflon or polyethylene.

[0011] As a preferred technical solution, the cone base is designed as a second-order frustum; the protrusion on the inner surface of the lower circle of the cone shell is fitted onto the shoulder of the small frustum of the cone base, the lower circle coincides with the large frustum of the cone base, and the cone shell and the cone base are tightly connected.

[0012] As a preferred technical solution, the conical head shell and the conical head base are connected by adhesive or welding.

[0013] As a preferred technical solution, the conical head shell and the conical head base are connected by fastening screws.

[0014] As a preferred technical solution, the protruding pipe is tapered.

[0015] As a preferred technical solution, the connection between the centrifuge tube body and the Luer cone head is a conical neck.

[0016] As a preferred technical solution, the core rod is connected to the core rod head via a threaded structure, and the core rod head is connected to the sealing elastic piston bayonet.

[0017] As a preferred technical solution, it also includes a fixing protective sleeve, which is divided into an upper cover and a lower cover. The upper cover is a tube open at one end, with a conical fixing seat at the top inside the upper cover, tube fixing ribs on the inner wall of the upper cover, a stop strip at the open end, and a protruding snap on the outer wall of the stop strip. The lower cover is a tube open at one end and is positioned opposite to the upper cover. A stop is provided at the open end of the lower cover, and a concave snap is provided on the inner wall of the stop. The upper cover and the lower cover are connected by the concave-convex snap structure.

[0018] The beneficial effects of this utility model are as follows:

[0019] This utility model's platelet-rich plasma preparation kit features a Luer cone at the end of a centrifuge tube. The Luer cone is surrounded by a slit-equipped elastic valve body that encloses a raised tube. The conduit of the liquid-conducting connector connects to the raised tube within the sealed elastic valve body, forming a closed passage. This avoids the contamination risk associated with open connections. Furthermore, the elastic valve body can quickly close when the liquid-conducting connector is separated, effectively preventing the risk of airborne bacteria contaminating the blood.

[0020] This utility model's platelet-rich plasma preparation kit features a conical constriction design for the centrifuge tube body and Luer cone tip. This design enhances the aggregation of the white film layer during platelet-rich plasma enrichment and reduces red blood cell contamination during extraction. The conical protrusion can be inserted into the conduit of the liquid connector to form a closed passage, further reducing the liquid flow space. This further improves the aggregation of the white film layer, enabling more precise extraction of the white film layer, enhancing the collection effect, reducing red blood cell contamination, and preventing blood from overflowing and remaining in the cone tip cavity.

[0021] This utility model's platelet-rich plasma preparation kit places centrifuge tubes in a fixed protective sleeve, which can increase the stability of the centrifuge tubes during centrifugation and also prevent the centrifuge tubes from directly contacting non-sterile centrifugation equipment during centrifugation, further improving infection control.

[0022] This utility model's platelet-rich plasma preparation kit is simple, convenient, and reliable in its preparation process, and produces platelet-rich plasma with high efficiency and purity. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a structural diagram of the centrifuge tube of this utility model;

[0025] Figure 2 This is a top view of a centrifuge tube;

[0026] Figure 3 A cross-sectional schematic diagram of the liquid-conducting connector of this utility model;

[0027] Figure 4 For the extraction operation connection structure diagram;

[0028] Figure 5 for Figure 4 Enlarged view of the area within the dashed box;

[0029] Figure 6 This is a structural diagram of the fixed protective sleeve of this utility model;

[0030] Figure 7 This is a bottom view of the top cover of the fixed protective sleeve of this utility model;

[0031] Figure 8 This is a diagram showing the application relationship between centrifuge tubes and fixed protective sleeves.

[0032] Reference numerals: 010-Centrifuge tube, 011-Tube body, 012-Inner wall of tube body, 013-Sealing elastic piston, 014-Core rod, 015-Core rod head, 016-Thread, 017-Bottom flange, 018-Neck, 020-Luer cone, 021-External thread, 022-Slit, 023-Elastic valve body, 024-Cone shell, 025-Cone base, 026-Protruding tubing, 027-Boss, 028-Shoulder of small truncated cone, 030-Fixing protective sleeve, 031-Cone fixing seat, 032-Top cover, 033-Tube body fixing rib, 034-Protruding bayonet, 035-Concave bayonet, 036-Bottom cover, 040-Liquid guide connector, 041-Clamping ring, 042-Internal thread, 043-Tube. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0034] In the description of this application, it should be understood that the orientations or positional relationships indicated by terms such as "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," and "outer" are based on the orientations or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature, unless otherwise explicitly specified.

[0035] A platelet-rich plasma preparation kit, such as Figures 1-8 As shown, it includes: centrifuge tube 010 and liquid delivery connector 040.

[0036] The centrifuge tube 010 has a Luer cone 020 with a slit 022 and an elastic valve body 023 at the upper front end of the tube body. The Luer cone 020 includes a cone shell 024 and a cone base 025. The cone base 025 is connected to the centrifuge tube body 011 and the cone base 025 is connected to the cone shell 024. The inner side of the cone shell 024 and the cone base 025 form a cavity. A protruding tube 026 is provided in the middle of the front end of the cone base 025. The elastic valve body 023 is enclosed in the cone shell 024. The outer surface of the elastic valve body 023 is adapted to the shape of the inner surface of the cone shell 024. The interior of the elastic valve body 023 is hollow and encloses the protruding tube 026. The end of the cone shell 024 away from the cone base 025 is provided with an external thread 021.

[0037] Preferably, the cone base 025 and the centrifuge tube 010 body 011 are integrally molded from a polymer material, preferably a highly hydrophobic polymer material such as polypropylene, Teflon, or polyethylene, to reduce the adhesion of the container to blood cell components. The cone base 025 is designed as a second-order frustum; the protrusion 027 on the lower inner surface of the cone shell 024 is fitted to the shoulder 028 of the small frustum of the cone base 025, and the lower circle coincides with the large frustum of the cone base 025. The cone shell 024 and the cone base 025 are fastened together, such as by adhesive, welding, or fastening screws.

[0038] The fluid guide connector 040 is a Luer external cone locking connector equipped with a rotatable internal thread 042 retaining ring 041, and contains an external cone Luer connector conduit 043. The retaining ring 041 is rotatably connected to the conduit 043. The fluid guide connector 040 has a symmetrical double-ended structure, such as... Figure 3 As shown. Preferably, the fluid guide connector 040 is injection molded, and the material is preferably a highly transparent polypropylene, Teflon, polyethylene, or other highly hydrophobic polymer material, which reduces the adhesion of the container to blood cell components during the preparation process and facilitates the observation of blood flow inside the tube.

[0039] The elastic deformation of the slit 022 on the Luer cone 020 at the top of the centrifuge tube 010 allows the tubing 043 on the fluid guide connector 040 to be inserted into the cavity of the Luer cone 020, forming a passage with the protruding tubing 026. Rotating the retaining ring 041 on the fluid guide connector 040 locks the external thread 021 of the Luer cone 020 at the top of the centrifuge tube 010 with its internal thread 042, which can be used for blood collection and PRP extraction.

[0040] Preferably, the protruding conduit 026 is tapered, and the protruding conduit 026 can be inserted into the conduit 043 to form a closed passage.

[0041] The connection between the centrifuge tube 010 body 011 and the Luer cone 020 is designed as a conical constriction 018. After centrifugation, platelet-rich plasma is divided into a plasma layer, a white film layer, and a red blood cell layer. The white film layer is where platelets are deposited and is very thin. The conical constriction 018 at the connection between the centrifuge tube body 011 and the Luer cone 020 can enhance the aggregation of the white film layer during platelet-rich plasma enrichment and reduce red blood cell contamination during extraction. The conical protrusion 026 is inserted into the conduit 043 of the liquid connector to form a closed passage, further reducing the liquid flow space, which can further improve the aggregation of the white film layer, extract the white film layer more accurately, improve the collection effect of the white film layer, reduce red blood cell contamination, and prevent blood from overflowing and remaining in the cone cavity.

[0042] When the fluid guide connector 040 separates from the Luer cone 020, the elastic deformation of the slit 022 of the elastic valve body 023 disappears, and it becomes closed, effectively preventing the risk of airborne bacteria contaminating the blood.

[0043] The centrifuge tube 010 has a rolled edge at the bottom (017) designed as a universal syringe rolled edge design. This makes it easier for users to hold the tube when drawing blood and to place it on the work surface after centrifugation.

[0044] Furthermore, the inner wall 012 of the centrifuge tube 010 is slidably connected to a sealing elastic piston 013, and the sealing elastic piston 013 is detachably connected to a core rod 014; preferably, the core rod 014 is connected to a core rod head 015 through a threaded structure 016, and the core rod head 015 is bayoneted with the sealing elastic piston 013, so that while pushing and pulling the core rod 014, the core rod head 015 and the sealing elastic piston 013 can slide along the inner wall 012 of the tube.

[0045] During use, the centrifuge tube 010 is separated from the liquid guide connector 040 after blood collection, and the core rod 014 is separated from the core rod head 015 through the threaded structure 016 to reduce the volume of the centrifuge tube 010 during centrifugation. The core rod head 015 is connected to the sealing elastic piston 013 by a bayonet to prevent the sealing elastic piston from deforming and displacing due to pressure during centrifugation, which would lead to seal failure.

[0046] Furthermore, it also includes a fixed protective sleeve 030 for accommodating centrifuge tubes 010; the fixed protective sleeve 030 is divided into an upper cover 032 and a lower cover 036. The upper cover 032 is a tube open at one end. A cone-shaped fixing seat 031 is provided at the top inside the upper cover 032. The inner wall of the upper cover 032 is provided with tube fixing ribs 033. A stop bar is provided at the open end. A convex snap 034 is provided on the outer wall of the stop bar. The lower cover 036 is a tube open at one end and is arranged opposite to the upper cover 032. A stop is provided at the open end of the lower cover 036. A concave snap 035 is provided on the inner wall of the stop. The upper cover 032 and the lower cover 036 are connected by the concave-convex snap structure.

[0047] Figure 6 As shown, the centrifuge tube 010 to be centrifuged is placed inside the lower cover 036, and the Luer cone 020 is fixed inside the cone fixing seat 031 of the upper cover 032. The tube body fixing rib 033 is fitted and fixed to the tube body 011 of the centrifuge tube 010, and the convex and concave locking slots 034 and 035 are tightened to prevent the centrifuge tube 010 from shaking. Placing the centrifuge tube 010 in the fixing protective sleeve 030 can increase the stability of the centrifuge tube 010 during centrifugation and can also avoid direct contact between the centrifuge tube 010 and non-sterile centrifugation equipment during centrifugation, further improving infection control.

[0048] This utility model discloses a platelet-rich plasma (PRP) preparation kit for use in the preparation of PRP.

[0049] The method for preparing platelet-rich plasma using the above-mentioned platelet-rich plasma preparation kit includes the following steps:

[0050] Step 1: Sample preparation: Connect the liquid guide connector 040 to the Luer cone 020 at the front end of the first centrifuge tube 010. Use the tubing 043 on the liquid guide connector 040 to pass through the slit 022 on the Luer cone 020 and insert it into the Luer cone 020 to communicate with the protruding tubing 026. The slit 022 seals the connection part through elastic deformation and is locked with a retainer 041. Connect the tubing 043 at the other end of the liquid guide connector 040 to the intravenous infusion needle (not shown) and lock it with a retainer 041. Draw whole blood to be centrifuged. After the drawing is completed, release the retainer 041 and separate the liquid guide connector 040 from the first centrifuge tube 010.

[0051] Step 2: First centrifugation: Remove the core rod 014 of the first centrifuge tube 010, place the first centrifuge tube 010 into the lower cover 036 of the fixed protective sleeve 030, place the Luer cone 020 into the cone fixing seat 031 of the upper cover 032 of the fixed protective sleeve 030 for fixation, and fit the tube body fixing rib 033 with the tube body 011 of the centrifuge tube 010 for fixation, and tighten the convex clamp 034 and concave clamp 035; then place the fixed protective sleeve 030 into the centrifuge and perform the first centrifugation operation, with a centrifugal force of 600-900g and a duration of 8-12 minutes; after centrifugation, the blood is divided into plasma layer, white membrane layer, and red blood cell layer from top to bottom;

[0052] Step 3: Red blood cell separation: Remove the protective sleeve 030 from the centrifuge and take out the first centrifuge tube 010 after centrifugation. Insert the tubing 043 on the liquid delivery connector 040 into the Luer cone 020 of the first centrifuge tube 010, and connect the tubing 043 at the other end of the liquid delivery connector 040 to the Luer cone 020 of the second centrifuge tube 010 in the same way.

[0053] Pull the core rod 014 of the second centrifuge tube 010 to extract the upper plasma layer and white membrane layer from the first centrifuge tube 010. When the white membrane layer has completely entered the second centrifuge tube 010 through the fluid guide connector 040 and catheter 043, which is about 2 / 5 to 3 / 5 of the whole blood volume, stop the extraction and separate the fluid guide connector 040 from the Luer cone 020 of the second centrifuge tube 010.

[0054] Step 4: Second centrifugation: Take the second centrifuge tube 010 from which the plasma layer and white membrane layer were extracted in Step 3, unscrew the core rod 014 of the second centrifuge tube 010 and place the centrifuge tube into the fixing protective sleeve 030. Then place the fixing protective sleeve 030 into the centrifuge and perform a second centrifugation operation. The centrifugal force is 800-1500g and the duration is 8-12 minutes. After centrifugation, the blood is separated into anemic platelet plasma in the upper layer and platelet-rich plasma in the lower layer.

[0055] Step 5: Final sampling: Remove the protective sleeve 030 from the centrifuge, take out the second centrifuge tube 010 after the second centrifugation, insert a standard disposable sterile syringe (not shown) into the Luhr cone 020 of the second centrifuge tube 010, extract the upper layer of anemic platelet plasma, and shake the remaining liquid well to obtain the prepared platelet-rich plasma.

[0056] Of course, there may be other embodiments of this utility model. Without departing from the spirit and essence of this utility model, those skilled in the art can make various corresponding changes and modifications based on this utility model, but these corresponding changes and modifications should all fall within the protection scope of the appended claims of this utility model.

Claims

1. A platelet-rich plasma preparation kit, characterized in that: include: Centrifuge tubes (010), liquid delivery connectors (040); The centrifuge tube (010) has a Luer cone (020) with a flexible valve body (023) containing a slit (022) at the upper front end of the tube body; the Luer cone (020) includes a cone shell (024) and a cone base (025), the cone base (025) is connected to the tube body (011) of the centrifuge tube (010), the cone base (025) is connected to the cone shell (024), the inner side of the cone shell (024) and the cone base (025) form a cavity, and a protruding pipe (026) is provided in the middle of the front end of the cone base (025). The elastic valve body (023) is enclosed in the conical shell (024). The outer surface of the elastic valve body (023) is adapted to the shape of the inner surface of the conical shell (024). The elastic valve body (023) is hollow inside and encloses the protruding pipe (026). The end of the conical shell (024) away from the conical base (025) is provided with an external thread (021). The inner wall (012) of the centrifuge tube (010) is sealed and slidably connected to the sealing elastic piston (013). The sealing elastic piston (013) is detachably connected to the core rod (014). The liquid guide connector (040) is a Luer external cone locking connector equipped with a rotatable internal thread (042) retainer (041), and a conduit (043) with an external cone Luer connector inside. The conduit (043) can be inserted into the Luer cone (020) through the slit (022) of the elastic valve body (023) and communicate with the protruding pipeline (026). The retainer (041) of the liquid guide connector (040) is connected to the cone shell (024). The liquid guide connector (040) has a symmetrical double-head structure.

2. The platelet-rich plasma preparation kit according to claim 1, characterized in that: The cone base (025) and the centrifuge tube (010) body (011) are integrally molded from polymer material.

3. The platelet-rich plasma preparation kit according to claim 2, characterized in that: The cone base (025) is made of polypropylene, Teflon or polyethylene.

4. The platelet-rich plasma preparation kit according to claim 1, characterized in that: The cone base (025) is designed as a second-order frustum; the boss (027) on the inner surface of the lower circle of the cone shell (024) is assembled to the shoulder (028) of the small frustum of the cone base (025), and the lower circle coincides with the large frustum of the cone base (025). The cone shell (024) and the cone base (025) are tightly connected.

5. The platelet-rich plasma preparation kit according to claim 4, characterized in that: The cone-shaped outer shell (024) and the cone-shaped base (025) are connected by adhesive or welding.

6. The platelet-rich plasma preparation kit according to claim 4, characterized in that: The cone head housing (024) and the cone head base (025) are connected by fastening screws.

7. The platelet-rich plasma preparation kit according to claim 1, characterized in that: The protruding pipe (026) is tapered.

8. The platelet-rich plasma preparation kit according to claim 7, characterized in that: The connection between the centrifuge tube body (011) and the Luer cone (020) is a conical neck (018).

9. The platelet-rich plasma preparation kit according to claim 1, characterized in that: The core rod (014) is connected to the core rod head (015) via a threaded structure (016), and the core rod head (015) is connected to the sealing elastic piston (013) via a bayonet.

10. The platelet-rich plasma preparation kit according to any one of claims 1 to 9, characterized in that: It also includes a fixed protective sleeve (030), which is divided into an upper cover (032) and a lower cover (036). The upper cover (032) is a tube with one end open. A cone head fixing seat (031) is provided at the top inside the upper cover (032). The inner wall of the upper cover (032) is provided with a tube body fixing rib (033). A stop strip is provided at the open end. A convex snap (034) is provided on the outer wall of the stop strip. The lower cover (036) is a tube with one end open and is arranged opposite to the upper cover (032). A stop is provided at the open end of the lower cover (036). A concave snap (035) is provided on the inner wall of the stop. The upper cover (032) and the lower cover (036) are connected by a concave-convex snap structure.

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