Aquatic live seedling sample submission sterile sampling bag

Abstract

The utility model discloses aquatic live seedling sends and examines aseptic sampling bag belongs to aquatic live seedling sampling equipment technical field, the sampling bag includes the bag body, the bag body left upper portion is provided with first screw joint, and is equipped with first screw seal cover, first screw joint is used as the sample inlet of breeding water and seedling, and after oxygen is hit, is sealed with first screw seal cover, the bag body right lower portion is provided with second screw joint, and is equipped with second screw seal cover, the bag body top is provided with sealing line, the bag body right side, is provided with filter screen at sealing line and second screw joint, filter screen divides bag body into left cavity and right cavity, left cavity stores breeding water and seedling, right cavity stores breeding water, and filter screen effectively isolates seedling in left cavity, the utility model discloses to the seedling of small, quantity many, convenient separation from breeding water, in addition, adopt pressure -resistant oxygenation structure, and seedling survival rate is promoted, adopt aseptic closed sampling, and support in situ detection.

Description

Technical Field

[0001] This utility model relates to the field of aseptic sampling bags for live aquatic seedlings, belonging to the technical field of live aquatic seedling sampling equipment. Background Technology

[0002] Live aquatic organisms are required for seedling quarantine, genetic breeding research, and cross-border e-commerce introduction. Among these:

[0003] Aquatic seedling quarantine: When sending live specimens for testing, the samples must be kept viable to avoid test errors caused by death during transit;

[0004] Genetic breeding research: Ensure DNA / RNA integrity during transportation (e.g., with the option of providing DNA stabilizing solution);

[0005] Cross-border e-commerce import of live animals: meets WOAH international live animal transportation standards, reducing customs clearance and quarantine risks.

[0006] Therefore, when sampling seedlings, it is necessary to bring the aquaculture water and oxygenation, and then separate the seedlings from the aquaculture water once they arrive at the laboratory. However, for some small (≤3cm) and numerous seedlings, separating them from the aquaculture water is very troublesome. Therefore, it is necessary to design a multifunctional sterile sampling bag suitable for live aquatic specimen testing to facilitate laboratory sampling. Utility Model Content

[0007] To address the above needs, this utility model designs a sterile sampling bag for sending live aquatic seedlings for testing, which aims to facilitate the separation of small and numerous seedlings from aquaculture water.

[0008] The purpose of this utility model is achieved as follows:

[0009] The sterile sampling bag for sending live aquatic seedlings for testing includes the bag body, a first spiral interface, a first spiral sealing cap, a second spiral interface, a second spiral sealing cap, a sealing line, and a filter screen;

[0010] The bag is provided with a first spiral interface on the upper left and equipped with a first spiral sealing cap. The first spiral interface serves as the inlet for aquaculture water and seedlings. After oxygen is introduced, it is sealed with the first spiral sealing cap.

[0011] The bag body is provided with a second spiral interface on the lower right side, and is equipped with a second spiral sealing cap;

[0012] A sealing line is provided at the top of the bag;

[0013] On the right side of the bag, a filter screen is provided at the sealing line and the second spiral interface. The filter screen divides the bag into a left cavity and a right cavity. The left cavity stores aquaculture water and seedlings, while the right cavity stores aquaculture water. The filter screen effectively isolates the seedlings in the left cavity.

[0014] The sterile sampling bags used for submitting live aquatic seedlings for testing have a filter screen of 1-3 mesh.

[0015] The aforementioned sterile sampling bags for live aquatic seedlings are transparent and have graduated lines printed on their surface.

[0016] The aforementioned sterile sampling bags for live aquatic seedlings are provided with handwritten labels on their surfaces.

[0017] The aforementioned sterile sampling bag for live aquatic seedlings has a flat surface at the bottom that allows it to stand upright.

[0018] The beneficial effects of this utility model of sterile sampling bag for live aquatic seedlings are as follows: Compared with the prior art, this application is suitable for seedlings that are small in length (≦3cm) and numerous, making it convenient to separate them from the aquaculture water; in addition, the use of a pressure-resistant oxygen-filling structure increases the seedling survival rate by 40%, and the use of sterile sealed sampling supports in-situ detection. Attached Figure Description

[0019] Figure 1 A schematic diagram of the sterile sampling bag for submitting live aquatic seedlings for testing according to this utility model. Figure 1 .

[0020] Figure 2 A schematic diagram of the sterile sampling bag for submitting live aquatic seedlings for testing according to this utility model. Figure 2 .

[0021] In the diagram: 1. Bag body, 2. First spiral interface, 3. First spiral sealing cap, 4. Second spiral interface, 5. Second spiral sealing cap, 6. Sealing line, 7. Filter screen. Detailed Implementation

[0022] The specific embodiments of this utility model will now be described in further detail with reference to the accompanying drawings. Specific Implementation Method 1

[0024] The schematic diagram of the sterile sampling bag for submitting live aquatic seedlings for testing in this specific implementation method is shown below. Figure 1 As shown, it includes a bag body 1, a first spiral interface 2, a first spiral sealing cap 3, a second spiral interface 4, a second spiral sealing cap 5, a sealing line 6, and a filter screen 7;

[0025] The bag body 1 is provided with a first spiral interface 2 on the upper left and is equipped with a first spiral sealing cap 3. The first spiral interface 2 serves as the inlet for aquaculture water and seedlings. After oxygen is introduced, it is sealed with the first spiral sealing cap 3.

[0026] The bag body 1 is provided with a second spiral interface 4 on the lower right side and is equipped with a second spiral sealing cap 5;

[0027] A sealing line 6 is provided at the top of the bag body 1;

[0028] On the right side of the bag body 1, a filter screen 7 is provided at the sealing line 6 and the second spiral interface 4. The filter screen 7 divides the bag body 1 into a left cavity and a right cavity. The left cavity stores aquaculture water and seedlings, and the right cavity stores aquaculture water. The filter screen 7 effectively isolates the seedlings in the left cavity.

[0029] The working principle is as follows:

[0030] Step a: Seal the second spiral interface 4 with the second spiral sealing cap 5;

[0031] Step b: The culture water and seedlings are put into the bag body 1 through the first spiral interface 2. The filter screen 7 isolates the seedlings in the left cavity, while the culture water fills the entire bag body 1.

[0032] Step c: Inject oxygen into bag 1 through the first spiral interface 2;

[0033] Step d: After oxygenation is completed, seal the first spiral interface 2 with the first spiral sealing cap 3;

[0034] Step e: Open the second spiral sealing cover 5 and release the aquaculture water from the second spiral interface 4, leaving only the seedlings in the left cavity;

[0035] Step f: Cut open the sealing line 6 and collect the seedlings from the left cavity. Specific Implementation Method Two

[0037] The sterile sampling bag for live aquatic seedlings under this specific embodiment, based on Specific Embodiment One, further specifies that the filter screen 7 is 1-3 mesh, as follows:

[0038] Table 1. Sizes and functions of different filter screen specifications

[0039] Specific Implementation Method 3

[0041] The schematic diagram of the sterile sampling bag for submitting live aquatic seedlings for testing in this specific implementation method is shown below. Figure 2 As shown, based on Specific Embodiment 1 or Specific Embodiment 2, the following further limitations are made: the bag body 1 is transparent and has scale lines printed on its surface; a handwritten label is provided on the surface of the bag body 1; and the bottom of the bag body 1 has a flat surface that allows the bag body 1 to stand upright.

[0042] In addition, the following information needs to be explained:

[0043] First, materials and selection

[0044] Sampling bag 1 is made of PP material, which is resistant to acids and alkalis and can withstand high-pressure sterilization (121℃).

[0045] The first spiral interface 2 is made of PP material, and the first spiral sealing cap 3 uses a GL45 universal interface, which can collect sample volumes of 1-20L. It can be used as an inlet for aquaculture water and seedlings, and can be sealed after oxygenation. The remaining parts are sealed with a sealing line.

[0046] The second spiral interface 4 is made of PP material;

[0047] The sampling bag 1 can be further subdivided into an upper living chamber and a lower water environment maintenance chamber. The upper living chamber is made of transparent flexible TPU material with a built-in honeycomb buffer layer to prevent fish fry from being damaged by collision. The lower water environment maintenance chamber contains water quality stabilizers (such as dissolved oxygen slow-release particles and ammonia nitrogen adsorbents), which are exchanged with the living chamber through a microporous membrane. A one-button rapid oxygenation interface can also be set on the bag body 1, which can maintain dissolved oxygen >5mg / L for more than 12 hours in actual tests.

[0048] Second, biosafety design

[0049] The bag has a pre-installed sterile sealed sampling tube on one side wall for direct water sampling and testing, avoiding contamination upon opening. It is also equipped with a slot for pathogen detection test strips, such as a rapid test strip for shrimp white spot virus.

[0050] Third, temperature visualization design

[0051] The bag body 1 has a built-in temperature-sensitive color-changing label that can monitor the transportation environment in real time within the range of 15℃-30℃.

[0052] Fourth, ecological compatibility design

[0053] The bag body 1 is made of biodegradable material: the outer layer is made of PBAT / PLA biodegradable composite material, and the inner layer is coated with medical-grade silicone, which meets the GB4806 food contact standard; an algal polysaccharide slow-release layer is added to the inner wall as an anti-stress coating to reduce the stress response of fish fry.

[0054] Fifth, technical parameters

[0055] Table 2. Technical parameters of the sterile sampling bag for live aquatic seedlings submitted for testing according to this utility model.

[0056]

[0057] Sixth, Application Scenarios

[0058] Table 3. List of application scenarios for the sterile sampling bag for submitting live aquatic seedlings of this utility model.

[0059]

[0060] Seventh, innovative comparison

[0061] Table 4 Comparison of traditional sampling bags and sampling bags of this utility model

[0062]

Claims

1. A sterile sampling bag for submitting live aquatic seedlings for testing, comprising a bag body (1), characterized in that, It also includes a first spiral interface (2), a first spiral sealing cap (3), a second spiral interface (4), a second spiral sealing cap (5), a sealing line (6), and a filter screen (7); The bag body (1) is provided with a first spiral interface (2) on the upper left and equipped with a first spiral sealing cap (3). The first spiral interface (2) serves as the inlet for aquaculture water and seedlings. After oxygen is introduced, it is sealed with the first spiral sealing cap (3). The bag body (1) is provided with a second spiral interface (4) on the lower right side and is equipped with a second spiral sealing cap (5); A sealing line (6) is provided at the top of the bag body (1); On the right side of the bag body (1), a filter screen (7) is provided at the sealing line (6) and the second spiral interface (4). The filter screen (7) divides the bag body (1) into a left cavity and a right cavity. The left cavity stores aquaculture water and seedlings, and the right cavity stores aquaculture water. The filter screen (7) effectively isolates the seedlings in the left cavity.

2. The aseptic sampling bag for submitting live aquatic seedlings for testing according to claim 1, characterized in that, The filter screen (7) is 1-3 mesh.

3. The aseptic sampling bag for submitting live aquatic seedlings for testing according to claim 1, characterized in that, The bag (1) is transparent and has scale lines printed on its surface.

4. The aseptic sampling bag for submitting live aquatic seedlings for testing according to claim 1, characterized in that, A handwritten label is provided on the surface of the bag (1).

5. The aseptic sampling bag for submitting live aquatic seedlings for testing according to claim 1, characterized in that, The bottom of the bag (1) has a flat surface that allows the bag (1) to stand upright.

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