Embedding box for congenital megacolon specimens

Abstract

The utility model discloses a kind of embedding boxes for congenital megacolon specimen, it is related to the field of tissue embedding technology.It includes box body with filter hole, circular ring, tight knob column, helical segmentation strip and box cover.Circular ring is fixed along inside of side plate, helical segmentation strip is coiled in spiral, one end is connected with the tight knob column of center, the other end is fixed in circular ring, can adjust interval with knob column rotation to hold or release specimen.Tight knob column contains shaft sleeve, shaft cap and split type knob spoon, through hexagonal structure cooperation and the bistable clamping of arc spring and clamping groove, the clutch and stable rotation of knob spoon and shaft cap are realized, control helical strip tightness.Box body, circular ring, helical strip etc.are provided with round hole to ensure reagent penetration, box cover buckle sealing, label area is provided in front.The design realizes specimen integration fixation, maintains morphological continuity, simplifies material taking, embedding process, improves pathological diagnosis efficiency and accuracy.

Description

Technical Field

[0001] This utility model relates to the field of tissue embedding application technology, specifically to an embedding cassette for a specimen of congenital megacolon. Background Technology

[0002] Agonistonic congenital megacolon, also known as Hirschsprung's disease (HSCR), is a common congenital malformation of the digestive tract in children characterized by the complete absence of ganglion cells in a portion of the distal intestine. Due to the absence of ganglion cells, the intestinal segment is in a state of persistent spasm, causing fecal stasis in the proximal colon, leading to thickening and dilation of that segment, resulting in a megacolon. A definitive diagnosis of HSCR requires histopathological examination. The histopathological changes in HSCR mainly involve the absence of ganglion cells in the myenteric plexus and submucosal plexus in the narrowed segment, but with increased thickness and number of exogenous nerve fibers.

[0003] The pathological gross sampling of congenital megacolon specimens is as follows: First, describe the color and texture of the intestinal tract and measure the length and diameter range of the intestinal tract (dilated segment, transition segment, and narrow segment); then, cut the intestinal tract along the longitudinal axis and take a segment about 0.3 cm wide; finally, cut the intestinal tract into several pieces about 1.5 cm long, mark the ends of each piece with staining solution, and pack them into a standard embedding box in sequence from the dilated segment to the narrow segment. The overall appearance and position of the intestinal tract before subsequent operations are recorded by taking pictures or drawing diagrams.

[0004] The main problems with the existing technology are: (1) Each piece of intestinal tissue needs to be stained and marked with the cut ends and embedding surface. After the intestinal specimen undergoes dehydration and other processes, the color will be removed to varying degrees, and the tissue will also be deformed and flipped to varying degrees. As a result, it is difficult to quickly and accurately find the required embedding surface and direction when embedding the tissue. That is, it is necessary to cut it into segments because it cannot be fixed as a whole, and there is displacement during the dehydration and paraffin infiltration process. (2) When embedding the tissue, it is necessary to restore the original lesion of the whole intestinal segment and its position. It is necessary to compare it with the photos or pictures taken or drawn at the time of sampling before embedding. However, sometimes it is difficult to restore the whole appearance due to the different degrees of deformation and flipping of the tissue after dehydration, clearing and paraffin infiltration. (3) When the tissue is cut and spread, there is a need to repeat the comparison process during embedding, which brings a lot of repetitive and tedious work to the whole slide preparation process. Moreover, the whole process may not be able to fully restore the whole appearance of the congenital megacolon specimen, which brings a considerable challenge to the pathological diagnosis of congenital megacolon. Utility Model Content

[0005] To address the problems of cumbersome procedures and the inability to perform a complete overview of congenital megacolon specimens in a single step in existing technologies, the inventors have developed and designed an embedding cassette for congenital megacolon specimens. This cassette enables rapid, integrated fixation of the specimen, maintaining morphological continuity while ensuring sufficient reagent penetration through the pores. This integrated processing method replaces traditional segmented operations, fundamentally solving the problem of comprehensive observation and improving the convenience, efficiency, and accuracy of pathological procedures.

[0006] Specifically, this utility model is implemented as follows:

[0007] An embedding cassette for a specimen of congenital megacolon, comprising:

[0008] The box body includes a bottom plate with filter holes and side plates forming the retaining edges.

[0009] A circular ring is installed upright along the inner side of the side panel and is fixedly connected to the box body at the connection point with the bottom plate or side panel.

[0010] The tension knob is vertically mounted at the center of the bottom plate of the box and can rotate around the mounting point;

[0011] The spiral dividing strips are spirally distributed strip structures that are spirally coiled inside the box. One end of the spiral dividing strips is connected to the tightening and loosening knob, and the other end is fixed to the inner side of the circular ring. The spiral dividing strips can be tightened or loosened depending on the direction and number of rotations of the tightening and loosening knob. When the spiral dividing strips are tightened, the spacing between adjacent spiral dividing strips becomes narrower, and when the spiral dividing strips are loosened, the spacing between adjacent spiral dividing strips becomes wider. In the loosened state, a specimen of congenital megacolon can be inserted, and in the tightened state, the inserted specimen of congenital megacolon can be held in place.

[0012] The lid is hinged to the rear side panel and can be fastened to the side panels to securely cover the box.

[0013] Preferably, the top of the tension knob is provided with a screw cap or a nut with a screw groove, which can be operated manually or directly with a tool to rotate the tension knob.

[0014] Preferably, the tension knob post passes through the bottom plate of the box body and extends downward out of the bottom plate. The part located below the bottom plate includes a threaded section and a handle. After being tightened by a threaded fastening ring, the tension knob post is installed on the bottom plate and can be rotated by turning the handle.

[0015] Preferably, the tension / loosening knob includes a bushing, a cap, and a knob key mounted on the center of the base plate. The middle part of the bushing is connected to the lower part of the base plate. The cap can be rotated while mounted on the bushing. The knob key can be inserted into the bushing from the lower part of the base plate and connected to the inner top of the cap at its end. The knob key can drive the cap to rotate synchronously. The rotation of the cap controls the tightness of the interval of the spiral dividing strip connected to the cap.

[0016] Preferably, the knob key and the shaft cap are separate designs. The inner top surface of the shaft cap is a hexagonal nut, and the top of the knob key is a matching hexagonal screw. The inner wall of the shaft sleeve is provided with two annularly distributed first concave grooves at different heights. The outer wall of the knob key is provided with several first arc-shaped spring pieces. The first arc-shaped spring pieces have outward elasticity and are adapted to the first concave grooves, and can be engaged in the first concave grooves. When the first arc-shaped spring pieces are engaged in the uppermost first concave groove, the top of the knob key... The hexagonal screw is inserted into the hexagonal nut; when the first arc-shaped spring is inserted into the first concave groove below, the hexagonal screw on the top of the knob key is disengaged from the hexagonal nut; the lower outer wall of the knob key is provided with several vertically distributed second concave grooves arranged around it, and the bottom edge of the bushing is provided with several second arc-shaped springs that are adapted to the second concave grooves. The second arc-shaped springs have an inward tightening elasticity and are adapted to the second concave grooves, and can be inserted into the second concave grooves to position the knob key.

[0017] Preferably, the circular ring, side plate, box cover, or spiral dividing strip are provided with a number of evenly distributed circular holes.

[0018] Preferably, the spiral dividing strip is a spring steel strip, and its elasticity is set such that under normal conditions, the spiral dividing strip can be rolled up and tightened under elastic force, so that the adjacent gaps become smaller; while when the tightening and loosening knob is subjected to force and rotated in the opposite direction, it can loosen the spiral dividing strip, so that the adjacent gaps become larger.

[0019] Preferably, the lid and the body are fitted with matching fasteners.

[0020] Preferably, a label area is provided on the outer side of the front side panel of the box body, where information strips for marking samples can be affixed or inserted.

[0021] Preferably, the spiral dividing strip is made of medical-grade stainless steel, or of engineering plastic material such as polyetheretherketone or polyphenylene sulfide.

[0022] The working principle of this invention is as follows: A circular ring, fixed to the bottom and side plates, is installed inside the box. A spirally wound segmented strip is connected to a central tightening knob on the bottom plate. Rotating the knob tightens or loosens the segmented strip, thus clamping and fixing the specimen of congenital megacolon. After the specimen is placed between the loosened spiral segmented strips, rotating the knob tightens the strip, fixing the specimen through elasticity or mechanical force and maintaining its morphological continuity. Simultaneously, the filter holes in the bottom plate, side plates, circular ring, and circular holes on the spiral segmented strips ensure sufficient penetration of dehydration, clearing, and paraffin-impregnation reagents, preventing specimen deformation and displacement. This design replaces traditional segmented operations with integrated fixation, allowing the specimen to maintain its original lesion position during embedding and sectioning processes. Combined with label markings and a sealed lid, it enables rapid and accurate overall observation, simplifying cumbersome procedures such as sample collection and embedding, and improving the efficiency and accuracy of pathological diagnosis.

[0023] Compared with the prior art, the working principle and beneficial effects of this utility model are as follows:

[0024] (1) Good specimen fixation and morphology maintenance: The integrated continuous fixation avoids segmental displacement. By tightly clamping the specimen with a spiral dividing strip, different intestinal segments such as dilated segments, transitional segments, and stenotic segments can be fixed as a whole in the box, replacing the traditional method of segmented cutting and individual embedding. This avoids tissue displacement and flipping caused by segmentation during dehydration and paraffin impregnation, maintains the original positional relationship of each segment of the intestine, and ensures that the whole lesion can be restored during pathological observation. The spiral dividing strip is made of spring steel or medical-grade material. The elastic design can distribute the force evenly during clamping and avoid excessive compression of the tissue. After being tightly wrapped, it is fixed by mechanical force, which can stabilize the specimen and reduce the damage to the tissue caused by traditional staining markers, and reduce the risk of deformation.

[0025] (2) Improved reagent penetration and processing efficiency: The porous structure ensures full reagent penetration. The filter holes on the bottom plate, side plates, circular rings, and spiral dividing strips of the box form a continuous structure, allowing dehydrating agents, clearing agents, paraffin, and other reagents to quickly and evenly penetrate to all parts of the specimen. This avoids uneven penetration caused by segmented embedding, shortens processing time, and improves slide preparation efficiency. There is no need to stain and mark the ends of each intestinal segment separately. After the specimen is fixed, the entire process of dehydration and embedding can be performed directly, reducing the need for repeated comparison of photographs or drawing steps. This avoids positioning difficulties caused by tissue deformation and simplifies the tedious segmented operation into "one-time fixation + overall processing", greatly reducing the complexity of pathological work.

[0026] (3) Improved accuracy and convenience of pathological diagnosis: A single slide allows for observation of the entire lesion. After the specimen is fixed as a whole, it can be continuously sliced ​​along the longitudinal axis during embedding, so that the pathological changes of the dilated segment, transition segment, and stenotic segment (such as absence of ganglion cells, thickening of nerve fibers, etc.) are presented on the same slide. There is no need to splice multiple slides, and the migration pattern of the lesion can be directly observed, improving diagnostic efficiency and accuracy. The label area at the front of the box can mark specimen information (such as patient number, intestinal segment location). Combined with the fixed order of the spiral dividing strips (from the dilated segment to the stenotic segment), there is no need to repeatedly compare the sampling photos during embedding. Slices can be directly sliced ​​according to the fixed positional relationship, reducing human judgment error, and is especially convenient for beginners.

[0027] (4) Advantages in saving consumables and practicality: It can reduce the use of embedding cassettes and slides. Traditional methods require cutting the intestinal segment into multiple pieces and packaging them into multiple embedding cassettes. This design only requires one embedding cassette through overall fixation, while reducing the number of slides (the same slide contains multiple lesions), thus reducing the cost of consumables. The spiral segment is made of medical-grade stainless steel or engineering plastics (such as polyetheretherketone, polyphenylene sulfide), which has acid and alkali resistance and corrosion resistance. It can adapt to chemical reagent environments such as dehydration and wax impregnation, and is not easily damaged after repeated use, resulting in low long-term cost.

[0028] This invention not only solves the problems of tissue displacement, uneven reagent penetration, and cumbersome slide splicing in traditional technologies, but also achieves "full-view visualization" in pathological diagnosis through structural innovation, fundamentally improving the efficiency and accuracy of pathological processing of congenital megacolon specimens, and has significant clinical application value. Attached Figure Description

[0029] Figure 1 A three-dimensional structural view of an embedding cassette used for a specimen of congenital megacolon;

[0030] Figure 2 A bottom-view three-dimensional structural view of an embedding cassette used for a specimen of congenital megacolon;

[0031] Figure 3 This is a schematic diagram of the exploded structure of an embedding cassette used for a specimen of congenital megacolon.

[0032] Figure 4 A schematic diagram showing the open state of the spiral dividing strips with the gaps widened;

[0033] Figure 5 This is a schematic diagram illustrating the procedure for placing a specimen.

[0034] Figure 6 A schematic diagram showing the tightened state where the gap between the spiral dividing strips narrows;

[0035] Figure 7 A schematic diagram showing the structure with circular holes in the circular ring and the spiral dividing strip;

[0036] Figure 8 This is an exploded view of the embedding box structure in Example 4;

[0037] Figure 9 This is a schematic diagram of the structure of the shaft cap with a hexagonal nut in Example 4;

[0038] Figure 10 This is a perspective view of the knob key structure in Example 4;

[0039] Figure 11 This is a three-dimensional view of the second arc-shaped spring piece on the bushing on the base plate in Embodiment 4;

[0040] Figure 12 This is a schematic cross-sectional view of the structure in Example 4 where the knob key is separated from the shaft cap;

[0041] Figure 13 This is a schematic cross-sectional view of the structure in Example 4 where the knob key and the shaft cap are combined.

[0042] Figure label:

[0043] 1—Box body, 11—Filter hole, 12—Bottom plate, 13—Side plate, 14—Circular ring, 15—Circular hole, 16—Box lid, 17—Snap fastener, 18—Label area;

[0044] 2—Tightening knob post, 20—Fasting ring sleeve, 21—Nut, 22—Handle, 23—Shaft sleeve, 24—Shaft cap, 25—Knob key, 26—Hex nut, 27—Hex screw, 281—First concave groove, 282—First arc-shaped spring, 291—Second concave groove, 292—Second arc-shaped spring;

[0045] 3—Spiral dividing strips;

[0046] 4—Specimen. Detailed Implementation

[0047] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0048] Example 1: An embedding cassette for a specimen of congenital megacolon, comprising: a cassette body 1, including a base plate 12 with filter holes 11 and side plates 13 forming a retaining edge; a circular ring 14, vertically mounted along the inner side of the side plate 13, and fixedly connected to the cassette body 1 at the connection point with the base plate 12 or the side plate 13; a tension knob post 2, vertically mounted at the center of the base plate 12 of the cassette body 1, capable of rotating around the mounting point; and spiral dividing strips 3, a spirally distributed strip structure, spirally coiled inside the cassette body 1, one end of which is connected to the tension knob post 2. The other end is fixed to the inner side of the circular ring 14; the spiral dividing strip 3 can be tightened or loosened according to the rotation direction and number of rotations of the tightening knob 2. When tightened, the distance between adjacent spiral dividing strips 3 becomes narrower, and when loosened, the distance between adjacent spiral dividing strips 3 becomes wider. In the loose state, the congenital megacolon specimen 4 can be placed in, and in the tight state, the placed congenital megacolon specimen 4 can be clamped; the box cover 16 is hinged to the rear side plate 13 and can be fastened with each side plate 13 to firmly cover the box body 1. Among them, the top of the tightening knob 2 is provided with a screw cap or a nut 21 with a screw groove, which can be operated directly by hand or tools to rotate the tightening knob 2.

[0049] When using:

[0050] 1. Preparation stage: Opening the box and loosening the spiral dividing strips

[0051] Operating steps: Release the seal of the buckle on the box body 1 and open the hinged box cover 16; manually or with a tool, in this embodiment a screwdriver is used. The top of the screw cap has a cross-shaped groove. The assistant or experimenter inserts the screwdriver into the cross-shaped groove and rotates the screw cap on the top of the tightening knob 2. The knob is rotated counterclockwise, and the tightening knob 2 rotates in the opposite direction to release the spiraled dividing strip, so that the adjacent spacing of the spiral dividing strip 3 becomes loose. The adjacent spacing increases from small to large, which should be at least wide enough to accommodate the intestinal specimen 4. After the spiral dividing strip 3 is loosened, an open spiral gap is formed. The circular ring 14 and the knob are used as fulcrums to ensure that the gap is expanded evenly, avoid local jamming, and facilitate the insertion of the specimen 4.

[0052] 2. Specimen Placement: Positioning and Continuity Maintenance

[0053] Operating Procedures: The assistant or experimenter keeps the screwdriver stationary. The experimenter, or another hand, places the congenital megacolon specimen 4, cut along the longitudinal axis, into the gaps of the spiral dividing strip 3. The specimen should consist of intestinal segments approximately 0.3 cm wide and 1.5 cm long, or the entire intestinal segment marked in sections. The segments should be arranged from dilated to constricted, with the inner wall of the intestinal segment (e.g., mucosal surface or specific observation surface) facing upwards, depending on the sampling requirements. Ensure that both ends of the specimen 4 are aligned with the axis of the box 1, i.e., consistent with the spiral direction of the dividing strip 3, avoiding folding or twisting, and maintaining the original positional relationship of each intestinal segment. For example, dilated segments should be near the knob post, and constricted segments near the circular ring 14. The spiral direction of the dividing strip 3 is adapted to the longitudinal axis of the intestinal segment, forming a uniform and fixable structure along the length of the intestinal segment. The circular ring 14 and side plates 13 limit the extension limit of the spiral dividing strip, while the filter holes 11 of the bottom plate 12 support the bottom of the specimen 4, preventing sagging and deformation.

[0054] 3. Clamping and fixing: Tightening the knob and evenly distributing pressure.

[0055] Operating steps: Rotate the tension knob 2 clockwise, or turn it clockwise using the bottom handle 22. In this embodiment, a screwdriver is used to rotate it clockwise, causing the spiral dividing strip 3 to wind tightly and tighten the tension knob 2. The specific installation design of the tension knob 2 is existing technology. For example, using a partially threaded design, after rotating it clockwise to tighten, the tension knob 2 remains firmly installed on the base plate 12, and at the same time, it can wind the spiral dividing strip 3 several times to achieve tightening. At this time, the adjacent spacing of the spiral dividing strip 3 gradually decreases to the limit, so that the spiral dividing strip 3 fits tightly against both sides of the intestinal tube, achieving fixation. Then, the tension knob 2 can be loosened; observe the state of the specimen 4 to ensure that there is no displacement or excessive compression. When the spiral dividing strip 3 is tightened, it generates radial clamping force, fixing the intestinal tube in the spiral gap. The threaded section of the knob 3 cooperates with the fastening ring of the base plate 12 to prevent loosening after rotation and ensure fixation stability.

[0056] 4. Sealing and Marking: Lid Closure and Information Recording

[0057] Operating steps: Fasten the lid 16 to the box body 1 along the hinge, and secure the buckles of the side plate 13 to form a sealed space; insert or affix the specimen 4 information strip (such as patient number, intestinal segment type, sampling time, etc.) to the label area 18 at the front of the box body 1 to ensure that it corresponds to the photo record taken at the time of sampling.

[0058] 5. Post-processing: Reagent permeation and integrated operation

[0059] Operating Procedures: Place the embedded cassette containing specimen 4 into a dehydrator, clearing agent, or paraffin infiltration device, and process according to standard procedures. Reagents permeate through the filter holes 11 of the base plate 12, the round holes 15 of the side plate 13, the round holes 15 of the circular ring 14, and the round holes 15 of the spiral dividing strip 3 to all parts of specimen 4, completing dehydration, clearing, and paraffin infiltration. During embedding, open the cassette lid 16, loosen the spiral dividing strip 3, and remove specimen 4 for direct embedding, or slice along the spiral direction, ensuring that the same slice contains continuous intestinal segments. The porous structure forms a three-dimensional permeation channel, avoiding the permeation dead zones of traditional segmented embedding. The specimen 4, fixed as a whole, can be sliced ​​directly in its original order during embedding, eliminating the need for segmented comparison and improving slide preparation efficiency.

[0060] The screw spacing is controlled by rotating the knob, enabling flexible operation of "loosening-placing-tightening," adapting to intestinal specimens of different sizes. The clamping force of the dividing strip can be finely adjusted by the knob to suit the clamping strength, fixing it with appropriate force to avoid tissue damage. The axial support structure formed by the circular ring 14 and the knob ensures that the specimen 4 is fixed along the longitudinal axis of the intestinal tract, maintaining the spatial continuity of the lesion segment. At the same time, the circular hole 15 design of all components ensures that reagents can penetrate without dead corners, avoiding incomplete dehydration or uneven paraffin impregnation. From sampling and fixation to embedding and sectioning, the specimen 4 does not need to be segmented, reducing tedious steps such as staining, marking, and photographic comparison. The structural design of the embedding box directly enables visualization of the entire lesion, which is especially suitable for continuous pathological observation of the "stenotic segment-transitional segment-dilated segment" of congenital megacolon.

[0061] Example 2: Based on Example 1, further optimization is made. The tension / loosening knob 2 passes through the bottom plate 12 of the housing 1 and extends downward beyond the bottom plate 12. The portion located below the bottom plate 12 includes a threaded section and a handle 22. After tightening with a threaded fastening ring 20, the tension / loosening knob 2 is installed on the bottom plate 12, and can be rotated by turning the handle 22. At this time, it is not necessary to use a screwdriver to operate the tension / loosening knob 2 from above, but can be operated from the bottom. It should be noted that the bottom plate 12 and the side plate 13 are not flush with each other. The bottom plate 12 has an inward recessed design, providing height space for the threaded section and the handle 22.

[0062] In the preferred embodiment, the circular ring 14, the side plate 13, the box cover 16, or the spiral dividing strip 3 are all provided with a number of evenly distributed circular holes 15, which can increase the number of permeation channels.

[0063] Example 3: Based on Example 1 or 2, the difference lies in that the spiral dividing strip 3 is made of spring steel with an elastic design. After heat treatment, the spring steel strip has a high elastic limit. Under normal conditions without external force, it remains curled due to internal stress, causing the adjacent gaps of the spiral dividing strip 3 to automatically decrease. When the tension knob 2 is rotated in the opposite direction, the external force overcomes the elastic restoring force of the spring steel, forcing the spiral dividing strip 3 to be stretched and unfolded, increasing the gap. After the external force is removed, the spring steel automatically returns to its curled state, thus achieving a tight fixation. This self-tightening state can generate a continuous clamping force on the inserted intestinal specimen 4, preventing the specimen 4 from shifting or flipping due to liquid flow or operational shaking during dehydration, paraffin impregnation, etc., maintaining its morphological continuity. The spiral dividing strip 3 is made of medical-grade stainless steel, but can also be made of engineering plastic materials such as polyetheretherketone or polyphenylene sulfide.

[0064] Example 4 differs from Example 3 in that:

[0065] The tension / loosening knob 2 includes a bushing 23, a cap 24, and a knob key 25 mounted on the center of the base plate 12. The middle of the bushing 23 extends to the lower part of the base plate 12. The cap 24 can be fitted onto the bushing 23 and rotate. The knob key 25 can be inserted into the bushing 23 from the lower part of the base plate 12 and is connected to the inner top of the cap 24 at its end. The knob key 25 can drive the cap 24 to rotate synchronously. The rotation of the cap 24 controls the tightness of the interval of the spiral dividing strips 3 connected to the cap 24. At this time, the end of the knob key 25 is fixedly connected to the cap 24. In use, the knob key 25 needs to be turned by hand to drive the cap 24 to rotate around the bushing 23. After the gap of the spiral dividing strips 3 is loosened to an appropriate distance, the specimen 4 is placed in with the other hand or by another operator. Then, the knob key 25 is slowly released, and the spiral dividing strips 3 automatically tighten and fix the specimen 4. Then, the specimen can be released.

[0066] In a preferred embodiment, the knob key 25 and the shaft cap 24 are designed as separate parts. The inner top surface of the shaft cap 24 is provided with a hexagonal nut 26, and the top of the knob key 25 is a matching hexagonal screw 27. The inner wall of the bushing 23 is provided with two annularly distributed first concave grooves 281 at different heights. The outer wall of the knob key 25 is provided with a plurality of first arc-shaped spring pieces 282. The first arc-shaped spring pieces 282 have outward elasticity and are adapted to the first concave grooves 281, and can be inserted into the first concave grooves 281.

[0067] When the first arc-shaped spring 282 is inserted into the uppermost first concave slot 281, the hexagonal screw 27 on the top of the knob key 25 is inserted into the hexagonal nut 26.

[0068] When the first arc-shaped spring piece 282 is inserted into the first concave groove 281 below, the hexagonal screw 27 on the top of the knob key 25 is dislodged from the hexagonal nut 26.

[0069] The lower outer wall of the knob key 25 is provided with a plurality of vertically distributed second concave grooves 291 arranged around it. The bottom edge of the bushing 23 is provided with a plurality of second arc-shaped spring pieces 292 that are adapted to the second concave grooves 291. The second arc-shaped spring pieces 292 have an inward tightening elasticity and are adapted to the second concave grooves 291, and can be inserted into the second concave grooves 291 to position the knob key 25.

[0070] In actual use, the operation can be completed by a single person. Hold the box 1 with one hand and the knob key 25 with the other, pushing it upwards so that an arc-shaped spring clip engages in the uppermost first recessed slot 281. Rotating the knob key 25 at this time causes the hexagonal screw 27 at the top of the knob key 25 to engage in the hexagonal nut 26, thus driving the shaft cap 24 to rotate synchronously. During rotation, the second arc-shaped spring clip 292 of the bushing 23 continuously moves from one second recessed slot 291 to the next... The purpose of the adjacent second concave slots 291 is to prevent the knob key 25 from retracting and rotating when the wrist angle is adjusted by releasing the knob key 25 midway. This acts like a ratchet, allowing the hand holding the knob key 25 to be released after continuous twisting to loosen the gap of the spiral dividing strip 3 to an appropriate distance. The knob key 25 and the shaft cap 24 are limited and fixed by the action of the second arc-shaped spring 292 and will not automatically tighten, so that the specimen 4 can be inserted. After specimen 4 is placed in place, pinch the knob key 25 and pull it down. When the first arc-shaped spring piece 282 is disengaged from the first concave groove 281 above and disengaged from the first concave groove 281 below, the hexagonal screw 27 on the top of the knob key 25 is disengaged from the hexagonal nut 26. The nut 21 loses its retaining force, and the spiral dividing strip 3 drives the nut 21 to rotate synchronously under its own elasticity, gradually tightening the gap, thereby fixing specimen 4. When it is necessary to remove the sample, the hexagonal screw 27 on the top of the knob key 25 is pushed back into the hexagonal nut 26 to engage, which will cause it to rotate in reverse and loosen.

[0071] In the daily work of the pathology department, batch operations can be achieved by quickly inserting and removing the knob key 25. The spiral dividing strips 3 of multiple embedding boxes can be pre-adjusted to a loose state for batch placement of specimens 4, thus improving work efficiency.

[0072] The above-described specific examples are for illustrative purposes only and are not intended to limit the scope of this invention. Those skilled in the art can make various simple deductions, modifications, or substitutions based on the concept of this invention.

Claims

1. An embedding cassette for a specimen of congenital megacolon, characterized in that... include: The box body (1) includes a bottom plate (12) with filter holes (11) and side plates (13) forming the baffles. A circular ring (14) is installed upright along the inner side of the side plate (13) and is fixedly connected to the box body (1) at the connection point with the bottom plate (12) or the side plate (13); The tension knob post (2) is vertically installed at the center of the bottom plate (12) of the box body (1) and can rotate around the mounting point; The spiral dividing strip (3) is a spirally distributed strip structure that is spirally coiled inside the box (1). One end of the spiral dividing strip (3) is connected to the tightening and loosening knob (2), and the other end is fixed to the inner side of the circular ring (14). The spiral dividing strip (3) can be tightened or loosened according to the rotation direction and number of rotations of the tightening and loosening knob (2). When the spiral dividing strip (3) is tightened, the distance between adjacent spiral dividing strips (3) becomes narrower. When the spiral dividing strip (3) is loosened, the distance between adjacent spiral dividing strips (3) becomes wider. In the loosened state, a specimen of congenital megacolon (4) can be placed in. In the tightened state, the specimen of congenital megacolon (4) can be clamped. The lid (16) is hinged to the rear side panel (13) and can be fastened to the side panel (13) to securely cover the box body (1).

2. The embedding cassette according to claim 1, characterized in that, The top of the tension knob (2) is provided with a screw cap or a nut (21) with a screw groove, which can be operated manually or directly with a tool to rotate the tension knob (2).

3. The embedding cassette according to claim 1, characterized in that, The tension knob (2) passes through the bottom plate (12) of the box (1) and extends downward out of the bottom plate (12). The part located below the bottom plate (12) includes a threaded section and a handle (22). After being tightened by a threaded fastening ring (20), the tension knob (2) is installed on the bottom plate (12) and can be rotated by turning the handle (22).

4. The embedding cassette according to claim 1, characterized in that, The tension / tightening knob (2) includes a bushing (23), a cap (24), and a knob key (25) installed on the center of the base plate (12). The middle part of the bushing (23) is connected to the lower part of the base plate (12). The cap (24) can be fitted onto the bushing (23) and rotated. The knob key (25) can be inserted into the bushing (23) from the lower part of the base plate (12) and connected to the inner top of the cap (24) at the end. The knob key (25) can drive the cap (24) to rotate synchronously. The rotation of the cap (24) controls the tightness of the interval of the spiral dividing strip (3) connected to the cap (24).

5. The embedding cassette according to claim 4, characterized in that, The knob key (25) and the shaft cap (24) are designed separately. The inner top surface of the shaft cap (24) is set as a hexagonal nut (26), and the top of the knob key (25) is a matching hexagonal screw (27). The inner wall of the bushing (23) is provided with two annularly distributed first concave grooves (281) at different heights. The outer wall of the knob key (25) is provided with a number of first arc-shaped spring pieces (282). The first arc-shaped spring pieces (282) have outward elasticity and are adapted to the first concave grooves (281) and can be inserted into the first concave grooves (281). When the first arc-shaped spring piece (282) is inserted into the uppermost first concave slot (281), the hexagonal screw (27) on the top of the knob key (25) is inserted into the hexagonal nut (26); When the first arc-shaped spring piece (282) is inserted into the first concave groove (281) below, the hexagonal screw (27) on the top of the knob key (25) is dislodged from the hexagonal nut (26); The lower outer wall of the knob key (25) is provided with a plurality of vertically distributed second concave grooves (291) arranged around it. The bottom edge of the bushing (23) is provided with a plurality of second arc-shaped spring pieces (292) that are adapted to the second concave grooves (291). The second arc-shaped spring pieces (292) have an inward tightening elasticity and are adapted to the second concave grooves (291), and can be inserted into the second concave grooves (291) to position the knob key (25).

6. The embedding cassette according to any one of claims 1-4, characterized in that, The circular ring (14), side plate (13), box cover (16) or spiral dividing strip (3) are all provided with a number of evenly distributed circular holes (15).

7. The embedding cassette according to claim 1, characterized in that, The spiral dividing strip (3) is a spring steel strip. Its elasticity is set so that under normal conditions, the spiral dividing strip (3) can be rolled up and tightened under elastic force, so that the adjacent gaps become smaller; while when the tightening and loosening knob (2) is subjected to force and rotates in the opposite direction, it can loosen the spiral dividing strip (3), so that the adjacent gaps become larger.

8. The embedding cassette according to claim 1, characterized in that, The lid (16) and the body (1) are fitted with matching fasteners (17).

9. The embedding cassette according to claim 1, characterized in that, The front side panel (13) of the box body (1) is provided with a label area (18) on the outside, which can be used to attach or insert information strips for marking samples.

10. The embedding cassette according to claim 1, characterized in that, The spiral dividing strip (3) is made of medical-grade stainless steel or engineering plastic material such as polyether ether ketone or polyphenylene sulfide.

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