Microfluidic chip and optical detection device based on natural Eucommia ulmoides fiber waveguide

Abstract

This invention discloses a microfluidic chip and optical detection device based on a natural Eucommia ulmoides fiber optical waveguide. The microfluidic chip includes a substrate, an input optical fiber, an output optical fiber, and an Eucommia ulmoides fiber optical waveguide. At least one through-slot is provided on the substrate, which serves as both an optical alignment channel and a guiding channel for the flow of the detected liquid. The middle portion of the through-slot is inclined along its length. The input optical fiber, the Eucommia ulmoides fiber optical waveguide, and the output optical fiber are correspondingly disposed within one of the through-slots. The two ends of the Eucommia ulmoides fiber optical waveguide are coupled to the input optical fiber and the output optical fiber, respectively, to form a detector. This invention provides a microfluidic optical detection device based on a natural Eucommia ulmoides fiber optical waveguide, which features a novel and environmentally friendly structure, flexible adaptability, and label-free detection capabilities, making it particularly suitable for wearable or portable liquid composition analysis scenarios.

Description

Technical Field

[0001] This utility model specifically relates to a microfluidic chip and optical detection device based on a natural Eucommia ulmoides fiber optical waveguide, belonging to the field of optical detection technology. Background Technology

[0002] In recent years, with the development of wearable technology and flexible electronics, optical sensors have played an increasingly important role in health monitoring, environmental detection, and personalized medicine. Compared with traditional electrochemical sensing methods, optical detection based on fluorescence or optical waveguide principles has significant advantages such as non-contact operation, high sensitivity, and parallel analysis of multiple parameters, making it particularly suitable for integration into micro wearable devices for continuous monitoring. In existing optical detection systems, microstructured optical waveguides or fluorescent conductive elements are typically introduced to achieve effective signal transmission and local amplification. These waveguide materials are mostly synthetic materials, such as polymer optical fibers, silica microwave conductors, and dye-doped nanofibers. However, these conventional waveguide materials still face the following technical bottlenecks in wearable and miniaturized applications: high rigidity and lack of flexibility, making them difficult to adapt to complex dynamic surfaces such as skin; complex manufacturing processes requiring multiple micro-nano fabrication, doping, and encapsulation steps, hindering large-scale, low-cost fabrication; strong dependence on external fluorescent materials or dyes, leading to issues such as photobleaching, toxicity, and insufficient stability; large size and heavy structure, making it difficult to achieve truly ultra-thin, miniaturized, and lightweight designs; and difficulty in integration with flexible substrates, affecting system integration and portability. Furthermore, current micro-detection systems face the dual demands of device miniaturization and functional integration. How to significantly reduce device size and weight while maintaining optical detection performance is one of the core challenges for the continued development of optical sensing technology in the wearable field. Utility Model Content

[0003] In view of the shortcomings of the existing technology, the main purpose of this utility model is to provide a microfluidic chip and optical detection device based on natural Eucommia ulmoides fiber optical waveguide.

[0004] To achieve the aforementioned objectives, the technical solution adopted by this utility model includes:

[0005] The first aspect of this utility model provides a microfluidic chip based on a natural Eucommia ulmoides fiber optical waveguide, comprising: a substrate, an input optical fiber, an output optical fiber, and an Eucommia ulmoides fiber optical waveguide. The substrate has at least one through-slot, which serves as both an optical alignment channel and a guiding channel for guiding the flow of detected liquid. The middle portion of the through-slot is an arc-shaped segment that bends downwards along the depth direction of the through-slot. One input optical fiber, one Eucommia ulmoides fiber optical waveguide, and one output optical fiber are correspondingly disposed within one through-slot. The input optical fiber, the Eucommia ulmoides fiber optical waveguide, and the output optical fiber achieve coaxial optical coupling through the arc-shaped segment.

[0006] A second aspect of this utility model provides a microfluidic optical detection device based on a natural Eucommia ulmoides fiber optical waveguide, comprising:

[0007] The microfluidic chip based on natural Eucommia ulmoides fiber waveguide;

[0008] In addition, a laser source, a filter, a spectrometer, and a liquid supply mechanism are provided. The laser source is optically connected to the input fiber optic cable, the filter is disposed between the output fiber optic cable and the spectrometer, and the liquid supply mechanism is controllably connected to the through-slot of the microfluidic chip.

[0009] Compared with the prior art, the advantages of this utility model include:

[0010] This utility model provides a microfluidic optical detection device based on natural Eucommia ulmoides fiber waveguide. It has a novel structure, is environmentally friendly, and has flexible adaptability and label-free detection capability. It is particularly suitable for wearable or portable liquid composition analysis scenarios.

[0011] This utility model provides a microfluidic optical detection device based on a natural Eucommia ulmoides fiber optical waveguide. It uses a precision groove inside the microfluidic chip as an optical connection structure to achieve glue-free and replaceable docking between the optical fiber and the natural plant waveguide. The optical waveguide is embedded in the microfluidic chip channel to achieve tight coupling between optical sensing and fluid transport. Furthermore, by providing a dedicated waste liquid container and cleaning channel, the waveguide area can be rinsed with water. The combination of filter and spectrometer achieves high signal-to-noise ratio detection. Attached Figure Description

[0012] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a schematic diagram of the overall structure of a microfluidic optical detection device based on a natural Eucommia ulmoides fiber optical waveguide, provided in a typical embodiment of this utility model.

[0014] Figure 2 This is a schematic diagram of the planar structure of a microfluidic chip based on a natural Eucommia ulmoides fiber optical waveguide, provided in a typical embodiment of this utility model.

[0015] Figure 3 This is a schematic diagram of the longitudinal cross-sectional structure of a microfluidic chip based on a natural Eucommia ulmoides fiber optical waveguide, provided in a typical embodiment of this utility model.

[0016] Figure 4 This is a microscopic image of the coupling end between the Eucommia ulmoides fiber optical waveguide and a single-mode optical fiber, provided in a typical embodiment of this utility model.

[0017] Figure 5 This is a schematic diagram of the liquid input structure in a typical embodiment of this utility model;

[0018] Figure 6 This is a schematic diagram of the structure of a Eucommia ulmoides rubber rod that blocks the liquid channel due to heat deformation in a typical embodiment of this utility model. Detailed Implementation

[0019] In view of the shortcomings of the prior art, the inventor of this case, through long-term research and extensive practice, has come up with the technical solution of this utility model. The following will further explain the technical solution, its implementation process, and its principles.

[0020] The first aspect of this utility model provides a microfluidic chip based on a natural Eucommia ulmoides fiber optical waveguide, comprising: a substrate, an input optical fiber, an output optical fiber, and an Eucommia ulmoides fiber optical waveguide. The substrate has at least one through-slot, which serves as both an optical alignment channel and a guiding channel for guiding the flow of detected liquid. The middle portion of the through-slot is an arc-shaped segment that bends downwards along the depth direction of the through-slot. One input optical fiber, one Eucommia ulmoides fiber optical waveguide, and one output optical fiber are correspondingly disposed within one through-slot. The input optical fiber, the Eucommia ulmoides fiber optical waveguide, and the output optical fiber achieve coaxial optical coupling through the arc-shaped segment.

[0021] Furthermore, the through-slot package includes a first slot segment, a second slot segment, and a third slot segment arranged sequentially along its own length direction. The first slot segment serves as the inflow segment for the detection liquid, and the third slot segment serves as the outflow segment for the detection liquid. At least a portion of the input optical fiber is disposed in the first slot segment, and at least a portion of the output optical fiber is disposed in the third slot segment. The Eucommia ulmoides fiber optical waveguide is disposed in the second slot segment. The first slot segment is parallel to the third slot segment, and the second slot segment slopes downward from the first slot segment toward the third slot segment. The downward slope direction of the second slot segment is the longitudinal direction of the through-slot.

[0022] Furthermore, the channel density of the effective area of ​​the microfluidic chip is 500 channels / cm2.

[0023] Furthermore, the substrate is provided with at least ten through slots.

[0024] Furthermore, the second groove segment is a downwardly curved arc-shaped groove.

[0025] Furthermore, the second groove segment is an S-shaped or spiral groove.

[0026] Furthermore, a filter screen is also provided on the substrate, which covers the opening of the first tank section for the detection liquid to enter, and a knob valve that can be opened and closed is also provided at the tail end of the third tank section.

[0027] Furthermore, the two ends of the Eucommia ulmoides fiber optical waveguide have a coupling structure, and the ends of the input optical fiber and the output optical fiber are embedded in the coupling structure and fixedly coupled to the Eucommia ulmoides fiber optical waveguide. The coupling structure is a microsphere structure formed by melting and deforming the end portion of the Eucommia ulmoides fiber optical waveguide and then solidifying it.

[0028] A second aspect of this utility model provides a microfluidic optical detection device based on a natural Eucommia ulmoides fiber optical waveguide, comprising:

[0029] The microfluidic chip based on natural Eucommia ulmoides fiber waveguide;

[0030] In addition, a laser source, a filter, a spectrometer, and a liquid supply mechanism are provided. The laser source is optically connected to the input fiber optic cable, the filter is disposed between the output fiber optic cable and the spectrometer, and the liquid supply mechanism is controllably connected to the through-slot of the microfluidic chip.

[0031] Furthermore, the liquid supply mechanism is connected to the microfluidic chip via a liquid input structure. The liquid input structure has a through liquid channel and a non-through cavity structure. The cavity structure is radially disposed on one side of the liquid channel and directly communicates with the liquid channel through an opening on the side wall of the liquid channel. The cavity structure contains a Eucommia ulmoides rubber rod, the axis of which intersects the axis of the liquid channel. The length of the cavity structure is slightly greater than or equal to the length of the Eucommia ulmoides rubber rod, and the width of the cavity structure is slightly greater than or equal to the width of the Eucommia ulmoides rubber rod. The Eucommia ulmoides rubber rod can undergo recoverable deformation with changes in ambient temperature. Furthermore, the Eucommia ulmoides rubber rod can bend towards the liquid channel at temperatures above 55°C, with a bending angle of 30°-90°. The bent portion can enter the liquid channel from the opening between the cavity structure and the liquid channel, blocking more than 50% of the cross-section of the liquid channel.

[0032] Furthermore, the contour shape of the radial cross-section of the liquid channel and the cavity structure is T-shaped.

[0033] Furthermore, in the first direction, the length of the cavity structure is greater than the radial length of the liquid channel, and in the second direction, the width of the cavity structure is equal to the radial width of the liquid channel. The first direction is perpendicular to the second direction and parallel to the axial direction of the Eucommia ulmoides adhesive rod.

[0034] Furthermore, the liquid input structure is connected to the first section of the through channel.

[0035] In a more specific implementation, the microfluidic optical detection device based on natural Eucommia ulmoides fiber waveguide further includes: a waste liquid collection container, which is connected to the tail end of the channel and is used to collect the liquid after detection.

[0036] The following will further explain the technical solution, its implementation process and principle in conjunction with the accompanying drawings and specific implementation examples. Unless otherwise specified, the filters, single-mode optical fibers, spectrometers and other components involved in the embodiments of this utility model are all known in the art, and their specific structures and models are not limited here.

[0037] Please refer to a typical implementation case. Figure 1 A microfluidic optical detection device based on natural Eucommia ulmoides fiber optical waveguide includes a laser source 100, a microfluidic chip 200, a filter 300, a spectrometer 400, and a liquid supply mechanism 500. The laser source 100 is optically connected to the microfluidic chip 200, and the microfluidic chip 200 is disposed between the laser source 100 and the spectrometer 400. The filter 300 is disposed between the microfluidic chip 200 and the spectrometer 400. The microfluidic chip 200 is optically connected to both the laser source 100 and the spectrometer 400. The liquid supply mechanism 500 is connected to the microfluidic chip 200.

[0038] Please refer to the following for details. Figure 1 and Figure 2 The microfluidic chip 200, as the core detection function component, includes a substrate 210, an input optical fiber 220, an output optical fiber 240, and a Eucommia ulmoides fiber waveguide 230. A through-slot 211 is provided on the substrate 210, which serves as both an optical alignment channel and a guide channel for guiding the flow of the detection liquid. The middle part of the through-slot 211 is inclined along its own length. The input optical fiber 220, the Eucommia ulmoides fiber waveguide 230, and the output optical fiber 240 are respectively arranged in the through-slot 211. The two ends of the Eucommia ulmoides fiber waveguide 230 are coupled to the input optical fiber 220 and the output optical fiber 240 to form a detector. The laser source 100 is optically connected to the input optical fiber 220, and the spectrometer 400 is optically connected to the output optical fiber 240. The liquid supply mechanism 500 is also connected to the through-slot 211, which serves as the guide channel.

[0039] Specifically, the laser source 100 serves as a fluorescence light source, primarily providing excitation light. Located at one end of the detection device, the laser source 100 is optically connected to the input fiber 220 to provide stable excitation light, exciting the natural fluorescence emission in the Eucommia ulmoides fiber waveguide 230, and serving as passive transmission light. For example, the excitation light provided by the laser source 100 can be light with a wavelength of 440 nm.

[0040] Specifically, the substrate 210 is mainly made of a transparent polymer and serves as a support platform for the detection module. For example, the transparent polymer can be PDMS or PMMA, etc.

[0041] Please refer to the following for details. Figure 2 and Figure 3 The dimensions of the through slot 211 match the connection end face of the Eucommia ulmoides fiber waveguide 230 and the output / output fiber 240. The through slot 211 serves as an optical alignment channel to precisely align the input fiber 220, the output fiber 240 and the Eucommia ulmoides fiber waveguide 230, ensuring effective coupling and transmission of light. At the same time, it serves as a liquid channel 720, allowing the liquid to be tested to contact the Eucommia ulmoides fiber waveguide 230 to form a near-field optical response. More specifically, the through-slot 211 includes a first slot segment 2111, a second slot segment 2112, and a third slot segment 2113 arranged sequentially along its length. The first slot segment 2111 serves as the inflow section for the detection liquid, and the third slot segment 2113 serves as the outflow section for the detection liquid. At least a portion of the input optical fiber 220 is disposed in the first slot segment 2111, and at least a portion of the output optical fiber 240 is disposed in the third slot segment 2113. The Eucommia ulmoides fiber waveguide 230 is disposed in the second slot segment 2112. The first slot segment 2111 and the third slot segment 2113 are parallel. The second slot segment 2112 is inclined downward from the first slot segment 2111 towards the third slot segment 2113, and the downward inclination direction of the second slot segment is the depth direction of the through-slot. Understandably, the input fiber 220 located in the first slot 2111 and the output fiber 240 located in the third slot 2113 are also arranged in parallel. The input fiber 220 and the output fiber 240 are precisely connected to the Eucommia ulmoides fiber waveguide 230 through the through slot 211.

[0042] Specifically, to better fit the Eucommia ulmoides fiber waveguide 230, the second groove segment 2112 is preferably configured as an arc-shaped groove segment that bends downwards along the longitudinal direction of the through groove. The arc-shaped groove allows the Eucommia ulmoides fiber waveguide 230 to fit and be fixed better. At the same time, the downwardly bent second groove segment 2112 can also better allow the liquid to be tested to pass through the second groove segment 2112. More specifically, to improve the contact reaction time between the Eucommia ulmoides fiber waveguide 230 and the liquid to be tested, the shape of the second groove segment 2112 in the transverse direction is preferably configured as S-shaped or spiral.

[0043] It should be noted that, Figure 2 The image only shows a substrate structure with one through-slot. Of course, more through-slots can be set on the substrate. The multiple through-slots have the same structure and the same direction. More specifically, the substrate can have at least ten through-slots. The through-slot density of the effective area of ​​the microfluidic chip is 500 slots / cm2.

[0044] Specifically, to prevent large particulate impurities from the liquid or the environment from affecting the detection results, a filter screen is also provided on the substrate 210 of the microfluidic chip 200. The filter screen covers the inlet of the first section for the detection liquid to enter, and a rotary valve that can be opened and closed is provided at the end of the third section. It should be noted that the material and porosity of the filter screen can be set according to specific needs and are not limited here. Similarly, the structure and model of the rotary valve can be selected according to specific needs and are not limited here.

[0045] Specifically, the Eucommia ulmoides fiber waveguide 230 is derived from natural Eucommia ulmoides trees, contains trans-polyisoprene, and is in the form of slender strips with a diameter of tens of micrometers. For example, the diameter of the Eucommia ulmoides fiber waveguide 230 is 1-10 μm, and its length is 1-5 cm, matching the fiber spacing. It is fixed in the middle region of the through-slot 211 and connected to two single-mode optical fibers through pre-alignment to form a complete optical waveguide channel. Specifically, through through-slot distribution optimization, the microfluidic chip can achieve a 1 cm... 2 One or more (e.g., 500) independent detection units can be integrated within the effective area, with the center-to-center spacing of the through-slots of each detection unit ≤200 μm. The test liquid comes into contact with the surface of the Eucommia ulmoides fiber, altering its optical environment and causing changes in fluorescence intensity or spectrum, reflecting the liquid's physicochemical properties (such as pH, ion concentration, etc.). Under 440 nm laser excitation, the Eucommia ulmoides fiber can generate natural fluorescence, which propagates along the waveguide to the output end and is detected by a spectrometer. Simultaneously, the refractive index and composition changes of the liquid in contact with the waveguide surface are sensed through the evanescent wave effect, enabling highly sensitive detection. Specifically, such as... Figure 4 As shown, the two ends of the Eucommia ulmoides fiber waveguide 230 have coupling structures. The ends of the input fiber 220 and the output fiber 240 are embedded in the coupling structures and fixedly coupled to the Eucommia ulmoides fiber waveguide 230. The coupling structures are microsphere structures formed by melting, deforming, and solidifying the end portions of the Eucommia ulmoides fiber waveguide 230. Specifically, both the input fiber 220 and the output fiber 240 are single-mode fibers, and the portions of the input fiber 220 and the output fiber 240 coupled to the Eucommia ulmoides fiber waveguide 230 are sharp, tapered sections.

[0046] Specifically, filter 300 is used to selectively filter out excitation light, allowing only fluorescence signals to pass through, thereby improving the detection signal-to-noise ratio and accuracy. For example, filter 300 can be a bandpass optical filter 300. Specifically, spectrometer 400 receives fluorescence and transmission optical signals via a single-mode optical fiber, detecting the intensities of the fluorescence and transmission optical signals from the Eucommia ulmoides fiber waveguide at two different wavelengths, thus achieving liquid state identification.

[0047] For details, please refer to Figure 5 and Figure 6 The liquid supply mechanism 500 is connected to the microfluidic chip 200 via a liquid input structure 700, specifically to the first segment of the through-slot 211. The liquid input structure 700 has a through liquid channel 720 and a non-through cavity structure 710. The cavity structure 710 is radially disposed on one side of the liquid channel 720 and directly communicates with it via an opening on the side wall of the liquid channel 720. Furthermore, the cavity structure 710 contains a Eucommia ulmoides rubber rod 800, the axis of which intersects the axis of the liquid channel 720. The length of the cavity structure 710 is slightly greater than or equal to the length of the Eucommia ulmoides rubber rod 800. The width of 710 is slightly greater than or equal to the width of the Eucommia ulmoides rubber rod 800. The Eucommia ulmoides rubber rod 800 can undergo recoverable deformation with changes in its ambient temperature. Furthermore, within a specific temperature range, the Eucommia ulmoides rubber rod 800 can bend and deform. A portion of the Eucommia ulmoides rubber rod 800 can enter the liquid channel 720 through the opening between the cavity structure 710 and the liquid channel 720 and block the liquid channel 720. Specifically, the Eucommia ulmoides rubber rod can bend and deform towards the liquid channel at temperatures above 55°C, with a bending angle of 30°-90°. The bent portion can enter the liquid channel through the opening between the cavity structure and the liquid channel and block more than 50% of the cross-section of the liquid channel. Exemplarily, the liquid supply mechanism 500 can be a storage solution or an injector, etc., without particular limitation.

[0048] Specifically, the radial cross-sectional contours of the liquid channel 720 and the cavity structure 710 are T-shaped. In the first direction, the length of the cavity structure 710 is greater than the radial length of the liquid channel 720. In the second direction, the width of the cavity structure 710 is equal to the radial width of the liquid channel 720. The first and second directions are in the same plane and perpendicular to each other. The first and second directions are perpendicular to the longitudinal direction of the channel, and the first direction is parallel to the axial direction of the Eucommia ulmoides gum rod 800. For example, in the first direction, the ratio of the length of the cavity structure 710 to the radial length of the liquid channel 720 is 3:1, and the ratio of the length to the width of the cavity structure 710 is 3:0.5. Specifically, Eucommia ulmoides gum has thermo-reversible deformation characteristics, and can undergo a transformation from a crystalline to an amorphous state within a set temperature range, accompanied by softening, expansion, or bending of the material. Based on this property, this invention uses Eucommia ulmoides gum to make Eucommia ulmoides gum rods as a temperature control valve structure, realizing automatic liquid opening and closing control without power supply.

[0049] For details, please refer to the following document again. Figure 1 The microfluidic optical detection device based on natural Eucommia ulmoides fiber waveguide also includes a waste liquid collection container. This container is connected to the tail end of the through-slot 211 and is used to collect the liquid after detection, thus preventing contamination of the detection area. After testing, the microfluidic detection chip can be rinsed with clean water to ensure the cleanliness and accuracy of repeated detections.

[0050] This invention provides a microfluidic optical detection device based on a natural Eucommia ulmoides gum fiber optical waveguide. This device is a flexible, small-volume optical sensing device. It utilizes natural Eucommia ulmoides gum fiber as an optical waveguide to realize a biosensor. The gum fiber comes from the bark of the Eucommia ulmoides tree, known as "plant gold," and is widely available, renewable, and biodegradable. The bark has a unique structure with many interconnected transparent gum fibers. The main component of these fibers is polyisoprene. Each fiber has a uniform diameter, a smooth surface, and almost no optical pores, making it suitable for use as an optical waveguide. A single Eucommia ulmoides gum fiber optical waveguide can be easily prepared using a physical pulling method. The gum fiber itself possesses natural fluorescence emission capabilities, eliminating the need for external dyes. Laser-induced fluorescence emission is used.

[0051] Specifically, the outer sheath and polymer layer of the single-mode fiber are removed to expose the inner core. Then, the fiber is tapered using a flame melting method to form a tapered structure. Next, the fiber surface is cleaned with acetone, alcohol, and deionized water to ensure cleanliness. Finally, the treated fiber is dried in a 45°C oven and then embedded in a through-slot 211 and then into a Eucommia ulmoides fiber waveguide 230. The through-slot 211 design facilitates coaxial connection between the single-mode fiber and the Eucommia ulmoides fiber waveguide 230, greatly improving optical coupling efficiency. When the test solution flows through the microfluidic channel across the surface of the plant fiber waveguide, target molecules in the solution enter the evanescent wave interaction region, thus affecting the fluorescence intensity of the plant fiber. By monitoring the changes in the fluorescence signal output from the other end of the plant fiber using a spectrometer 400, real-time, label-free detection of the solution composition or concentration can be achieved. Eucommia ulmoides fiber has a soft structure and good mechanical flexibility and skin compliance. It can be integrated with microfluidic chip 200 and attached to human skin to realize wearable sensing function. Combined with the evanescent wave mechanism in the waveguide, it can perform highly sensitive optical detection of trace liquids (such as sweat and biological samples) that are in contact with or close to the waveguide surface.

[0052] The working principle of the microfluidic optical detection device based on the optical waveguide of natural Eucommia ulmoides filaments provided in this embodiment is as follows: First, the fluorescence of Eucommia ulmoides filaments is excited by laser, and then the detection of the test liquid is achieved by utilizing the influence of the test liquid on the fluorescence. The wavelength of the fluorescence is inconsistent with the wavelength of the excitation light.

[0053] This utility model provides a microfluidic optical detection device based on natural Eucommia ulmoides fiber waveguide. It has a novel structure and is environmentally friendly. The microfluidic chip has been tested and found to have a bending radius of up to 5 mm, which can be adapted to the curvature of human skin (average curvature radius of 10-20 mm). It has flexible adaptability and label-free detection capability, and is particularly suitable for wearable or portable liquid component analysis scenarios.

[0054] The application areas of the microfluidic optical detection device based on natural Eucommia ulmoides fiber waveguide provided by this utility model embodiment include: pH sensing, where the surface of the Eucommia ulmoides fiber waveguide is in direct contact with the liquid, and its evanescent wave field is sensitive to changes in the hydrogen ion concentration in the liquid, which is reflected in the fluorescence intensity or spectrum; achieving real-time, label-free, and highly sensitive acid-base detection; specifically, it can be applied to the monitoring of sweat, biological samples, drinking water, and industrial liquids.

[0055] The potential application areas of the microfluidic optical detection device based on the natural Eucommia ulmoides fiber waveguide provided by this utility model embodiment include: bacterial sensing and human health monitoring. Eucommia ulmoides is a traditional Chinese medicine containing natural bioactive components (such as chlorogenic acid and lignans), which may specifically interact with certain bacteria or metabolites. The waveguide surface adsorption or response mechanism can be utilized to design it to selectively adsorb bacteria or their metabolites on the surface. The presence and concentration of bacteria can be indirectly determined by fluorescence intensity or transmitted light intensity. It is expected to realize a label-free, biocompatible pathogen monitoring platform.

[0056] It should be understood that the above embodiments are merely illustrative of the technical concept and features of this utility model, and are intended to enable those skilled in the art to understand the content of this utility model and implement it accordingly. They should not be construed as limiting the scope of protection of this utility model. All equivalent changes or modifications made in accordance with the spirit and essence of this utility model should be covered within the scope of protection of this utility model.

Claims

1. A microfluidic chip based on a natural Eucommia ulmoides fiber waveguide, characterized in that, include: The device comprises a substrate, an input optical fiber, an output optical fiber, and a Eucommia ulmoides fiber waveguide. The substrate has at least one through-slot, which serves as both an optical alignment channel and a guide channel for guiding the flow of the detection liquid. The middle portion of the through-slot is an arc-shaped groove segment that bends downwards along the depth direction of the through-slot. One input optical fiber, one Eucommia ulmoides fiber waveguide, and one output optical fiber are correspondingly disposed within one through-slot. The input optical fiber, the Eucommia ulmoides fiber waveguide, and the output optical fiber achieve coaxial optical coupling through the arc-shaped groove segment.

2. The microfluidic chip based on natural Eucommia ulmoides fiber waveguide according to claim 1, characterized in that: The through-slot includes a first segment, a second segment, and a third segment arranged sequentially along its length. The first segment serves as the inflow segment for the detection liquid, and the third segment serves as the outflow segment for the detection liquid. At least a portion of the input optical fiber is disposed in the first segment, and at least a portion of the output optical fiber is disposed in the third segment. The Eucommia ulmoides fiber waveguide is disposed in the second segment. The first segment and the third segment are parallel. The second segment slopes downward from the first segment to the third segment, and the downward slope of the second segment is the depth direction of the through-slot. And / or, the channel density of the effective area of ​​the microfluidic chip is 500 channels / cm2; And / or, the substrate is provided with at least ten through slots.

3. The microfluidic chip based on natural Eucommia ulmoides fiber waveguide according to claim 2, characterized in that: The second groove segment is a downward-curving arc-shaped groove.

4. The microfluidic chip based on natural Eucommia ulmoides fiber waveguide according to claim 2, characterized in that: The second groove segment is an S-shaped or spiral groove.

5. The microfluidic chip based on natural Eucommia ulmoides fiber waveguide according to claim 2, characterized in that: The substrate is also provided with a filter screen, which covers the opening of the first tank section for the detection liquid to enter, and a rotary valve that can be opened and closed is also provided at the tail end of the third tank section.

6. The microfluidic chip based on natural Eucommia ulmoides fiber waveguide according to claim 1, characterized in that: The two ends of the Eucommia ulmoides fiber optical waveguide have a coupling structure. The ends of the input optical fiber and the output optical fiber are embedded in the coupling structure and fixedly coupled to the Eucommia ulmoides fiber optical waveguide. The coupling structure is a microsphere structure formed by melting and deforming the end portion of the Eucommia ulmoides fiber optical waveguide and then solidifying it.

7. A microfluidic optical detection device based on a natural Eucommia ulmoides fiber optical waveguide, characterized in that, include: The microfluidic chip based on natural Eucommia ulmoides fiber waveguide as described in any one of claims 1-6; In addition, a laser source, a filter, a spectrometer, and a liquid supply mechanism are provided. The laser source is optically connected to the input fiber optic cable, the filter is disposed between the output fiber optic cable and the spectrometer, and the liquid supply mechanism is controllably connected to the through-slot of the microfluidic chip.

8. The microfluidic optical detection device based on natural Eucommia ulmoides fiber waveguide according to claim 7, characterized in that: The liquid supply mechanism is connected to the microfluidic chip via a liquid input structure. The liquid input structure has a through liquid channel and a non-through cavity structure. The cavity structure is radially disposed on one side of the liquid channel and directly communicates with the liquid channel through an opening on the side wall of the liquid channel. The cavity structure contains a Eucommia ulmoides rubber rod, the axis of which intersects the axis of the liquid channel. The length of the cavity structure is slightly greater than or equal to the length of the Eucommia ulmoides rubber rod, and the width of the cavity structure is slightly greater than or equal to the width of the Eucommia ulmoides rubber rod. The Eucommia ulmoides rubber rod can undergo recoverable deformation with changes in ambient temperature. Furthermore, the Eucommia ulmoides rubber rod can bend towards the liquid channel at temperatures above 55°C, with a bending angle of 30°-90°. The bent portion can enter the liquid channel from the opening between the cavity structure and the liquid channel, blocking more than 50% of the cross-section of the liquid channel.

9. The microfluidic optical detection device based on natural Eucommia ulmoides fiber waveguide according to claim 8, characterized in that: The contour shape of the radial cross section of the liquid channel and the cavity structure is T-shaped; And / or, in a first direction, the length of the cavity structure is greater than the radial length of the liquid channel, and in a second direction, the width of the cavity structure is equal to the radial width of the liquid channel, the first direction is perpendicular to the second direction, and the first direction is parallel to the axial direction of the Eucommia ulmoides adhesive rod; And / or, the liquid input structure is connected to the first section of the through channel.

10. The microfluidic optical detection device based on natural Eucommia ulmoides fiber waveguide according to claim 8, characterized in that, Also includes: A waste liquid collection container is connected to the tail end of the through-channel and is used to collect the liquid after testing.

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