An assembled high-throughput immunochromatographic test strip array sample application device

Abstract

This invention provides an assembled high-throughput immunochromatographic test strip array sample application device, comprising a 96-well plate fixing bracket (1) and a test strip support plate (2); immunochromatographic test strips (3) are arranged and fixed by slots (4) and limiting blocks (5) on the test strip support plate (2), with their sample application areas (6) protruding from the test strip support plate (2); multiple test strip support plates (2) are inserted into the slots (7) of the 96-well plate fixing bracket (1) to form a multi-channel immunochromatographic test strip array sample application device. This device can be used for high-throughput sample application in immunochromatographic detection.

Description

Technical Field

[0001] This invention provides an ultra-high throughput lateral flow immunochromatographic test strip detection device, belonging to the field of high throughput point detection technology. Background Technology

[0002] Lateral flow chromatography is a simple, fast, easy-to-use, and low-cost rapid testing technique that can obtain results on-site in a short time (5-15 minutes). It is widely used in clinical diagnosis, health inspection, epidemic prevention inspection, and military testing. However, with the development of this technology and the continuous expansion of its application scenarios, traditional multi-channel detection techniques are completely unable to meet the high-throughput and high-efficiency testing needs when faced with massive sample testing demands, such as the screening and testing of large numbers of samples during the pandemic.

[0003] Existing high-throughput detection technologies are mostly single-sample, multi-index combined detection, which increases the number of detection items by coating multiple detection reagents on the test strip, such as patents CN111089956A and CN118655309A. The disadvantages are that the antibody loading is limited, generally 3-5, and there is interference between reagents, so its application is relatively limited.

[0004] Another technology increases the number of channels on the test strip, with one sample well corresponding to multiple channels. This is also used for the joint detection of multiple indicators. Generally, the number of channels is 5-10, otherwise the size will be too large, such as patents CN216900570U, CN1768267A, and CN209117696U.

[0005] Another option is a single-channel test card, which allows for independent sample loading and simultaneous testing, and analysis using a multi-channel detector. The commonly used throughput is 8-10 samples, such as patents CN215339892U, CN110568203A, and CN209656713U. However, the sample loading process is very time-consuming.

[0006] When faced with a large number of samples to be tested simultaneously, such as hundreds or even more, the detection efficiency of the above-mentioned technologies will be very low, requiring a large number of repetitive operations, which is quite time-consuming, and consistency is also difficult to guarantee.

[0007] In some high-throughput screening experiments, 96-well plate-based assays are very common, and often multiple 96-well plates are used simultaneously. A typical example is immunoassay techniques such as ELISA, which require the parallel processing of a large number of 96-well plates. The entire process must be carried out under specialized laboratory conditions, which is time-consuming and labor-intensive, and does not meet the needs of real-time testing. Summary of the Invention

[0008] The purpose of this invention is to solve the problems of low efficiency and poor repeatability of ultra-high throughput detection in existing lateral flow detection technology. Based on the 96-well plate commonly used in the detection field, an ultra-high throughput lateral flow chromatography array sample loading device is proposed.

[0009] The technical solution of this utility model is as follows:

[0010] An assembled high-throughput immunochromatographic test strip array sample application device includes a 96-well plate fixing bracket and a test strip support plate; the immunochromatographic test strips are arranged and fixed by the locking and limiting blocks on the test strip support plate, and the test strip application area protrudes from the test strip support plate; multiple test strip support plates are inserted into the slots of the 96-well plate fixing bracket to form a multi-channel immunochromatographic test strip array sample application device.

[0011] The assembly method of this device is to assemble the 96-well plate fixing bracket and the 96-well plate in a vertical or horizontal arrangement, and then insert the test paper support plate into the 96-well plate fixing bracket to complete the assembly.

[0012] The internal frame of the 96-well plate fixing bracket has a width of 80mm, a length of 125mm, and a height of 50mm-100mm. The inner perimeter is provided with a slot, the width of which is adapted to the thickness of the test paper support plate, so that the latter can be stably inserted into it. The test paper support plate is adjustable up and down to adjust the depth of the test paper immersion in the sample interface. When the sample is absorbed and the liquid level drops continuously until it breaks contact with the test paper, the sample addition is completed.

[0013] The test strip support plate is 2-5mm thick and has slots and limiting blocks for fixing the immunochromatographic test strips. The slots are used to fix the immunochromatographic test strips, and their spacing is the same as the width of the immunochromatographic test strips. The limiting blocks are used to ensure that the immunochromatographic test strips are arranged neatly and that the sample application area of ​​the test strip can extend 5-10mm beyond the test strip support plate.

[0014] As a further improvement of this utility model, when the device is assembled with a 96-well plate, the number of detection channels can be changed by adding or removing the test paper support plate. Multiple 96-well plates can also be used together, or it can be used together with a 384-well plate.

[0015] As a further improvement of this invention, the immunochromatographic test strip also includes a lateral flow chromatography test strip for nucleic acid molecules.

[0016] This utility model has at least one of the following beneficial effects:

[0017] The assembled immunochromatographic test strip array sample loading device is installed on a 96-well plate. All sample loading areas of the test strip can simultaneously contact the samples in each well of the 96-well plate. The high efficiency of sample loading and detection of all samples is completed through capillary effect, which has a higher detection throughput than existing technologies. The efficiency is improved by nearly 10 times in the same operation time.

[0018] The depth of the sample application area of ​​the test strip into the sample can be adjusted to control the sample amount, eliminating the need for traditional dripping operations. This not only greatly reduces the workload but also ensures the consistency and repeatability of the sample amount.

[0019] It can be configured in combination to flexibly adjust the detection throughput, and can measure multiple indicators in the same sample or measure the same indicator in multiple samples. It has a short detection time and high efficiency, and is widely applicable to scenarios that require the immediate detection of a large number of samples, such as clinical testing, health testing, epidemic prevention testing, scientific research or drug high-throughput screening. It can also be placed in a 96-well automated mobile testing platform to complete the automated detection of ultra-high throughput samples. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of this utility model.

[0021] Figure 2 Top view of this utility model.

[0022] Figure 3 Side view of this utility model.

[0023] Figure 4 Front view of this utility model.

[0024] Figure 5 Schematic diagram of the 96-hole plate fixing bracket of this utility model.

[0025] Figure 6 Schematic diagram of the test paper support plate structure of this utility model.

[0026] In the diagram: 1. 96-well plate fixing bracket; 2. Test strip support plate; 3. Immunochromatographic test strip; 4. Test strip support plate locking position; 5. Test strip support plate limiting block; 6. Test strip sample application area; 7. 96-well plate fixing bracket slot; 8. 96-well plate. Detailed Implementation

[0027] To more clearly illustrate the embodiments of this utility model, the specific implementation methods of this utility model will be described below with reference to the accompanying drawings. Obviously, those skilled in the art can obtain other drawings and other implementation methods based on these drawings without any creative effort. Example

[0028] Reference Figures 1-6This invention proposes an assembled high-throughput immunochromatographic test strip array sample loading device, comprising a 96-well plate fixing bracket and 12 test strip support plates. Eight immunochromatographic test strips are fixedly arranged on the test strip support plates by means of locking and limiting the test strip support plates, and the eight test strip loading areas protrude 10mm from the test strip support plates. The 12 test strip support plates are inserted side by side into the slots of the 96-well plate fixing bracket and fixed to form a 96-channel immunochromatographic test strip array sample loading device. The assembled array sample loading device is installed on the 96-well plate, and the 96 test strip loading areas simultaneously contact the samples in the 96 wells of the 96-well plate, completing high-throughput sample loading and detection through the capillary effect of the test strips.

[0029] In this embodiment, the immunochromatographic test strip is a standard 8cm strip, with the sample application area extending approximately 3.5mm into the sample liquid surface, and the sample volume is approximately 100μL. Example

[0030] This invention proposes an assembled high-throughput immunochromatographic test strip array sample loading device, comprising a 96-well plate fixing bracket and eight test strip support plates. Twelve immunochromatographic test strips are arranged and fixed on the test strip support plates through locking and limiting obstructions. The twelve test strip loading areas protrude 5 mm from the test strip support plates. The eight test strip support plates are inserted side by side into the slots of the 96-well plate fixing bracket for fixation, forming a 96-channel immunochromatographic test strip array sample loading device. The assembled array sample loading device is installed on the 96-well plate, and the 96 test strip loading areas simultaneously contact the samples in the 96 wells of the 96-well plate, completing high-throughput sample loading and detection through the capillary effect of the test strips.

[0031] In this embodiment, the immunochromatographic test strip is a standard 6cm short strip, with the sample application area extending approximately 2mm into the sample liquid surface, and the sample volume is approximately 56μL.

[0032] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. An assembled high-throughput immunochromatographic test strip array sample application device, characterized in that: It includes a 96-well plate fixing bracket (1) and a test strip support plate (2); the immunochromatographic test strips (3) are arranged and fixed through the slots (4) and limiting blocks (5) on the test strip support plate (2), and the test strip sample application area (6) protrudes from the test strip support plate (2); multiple test strip support plates (2) are inserted into the slots (7) of the 96-well plate fixing bracket (1) to form a multi-channel immunochromatographic test strip array sample application device.

2. The assembled high-throughput immunochromatographic test strip array sample application device according to claim 1, characterized in that: The assembly method is to assemble the 96-well plate fixing bracket (1) and the 96-well plate (8) in a vertical or horizontal manner, and then insert the test paper support plate (2) into the 96-well plate fixing bracket (1) to complete the assembly.

3. The assembled high-throughput immunochromatographic test strip array sample application device according to claim 1, characterized in that: The 96-well plate fixing bracket (1) has an internal frame with a width of 80mm, a length of 125mm, and a height of 50mm-100mm; the inner perimeter is provided with a slot (7) with a width that matches the thickness of the test paper support plate (2), so that the latter can be stably inserted into it; The test paper support plate (2) is adjustable up and down to adjust the depth of the test paper immersion in the sample interface. When the sample is absorbed and the liquid level drops continuously until it breaks contact with the test paper, the sample addition is completed.

4. The assembled high-throughput immunochromatographic test strip array sample application device according to claim 1, characterized in that: The test strip support plate (2) is 2-5mm thick and has a locking position (4) for fixing the immunochromatographic test strip (3) and a limiting block (5). The locking position (4) is used to fix the immunochromatographic test strip (3) and the spacing between them is the same as the width of the immunochromatographic test strip (3). The limiting block (5) is used to ensure that the immunochromatographic test strip (3) is arranged neatly and that the test strip sample application area (6) can extend 5-10mm beyond the test strip support plate (2).

5. The assembled high-throughput immunochromatographic test strip array sample application device according to claim 1, characterized in that: The number of detection channels can be changed by increasing or decreasing the number of test strip support plates (2).

6. The assembled high-throughput immunochromatographic test strip array sample application device according to claim 1, characterized in that: The immunochromatographic test strip (3) also includes a lateral flow chromatography test strip for nucleic acid molecules.

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