A cell culture plate

Abstract

The utility model provides a kind of cell culture plate, belong to biological experimental equipment technical field.It solves the problem that the smaller sample spacing multichannel pipettor sampling or sampling cannot be realized on the cell culture multi-well plate with larger single-hole area in prior art.This cell culture plate includes a plurality of row-column distribution culture holes plate body, a plurality of culture holes are divided into multiple groups of culture holes with two columns as a group in sequence, the position of adjacent and parallel two culture holes in each group of culture holes is staggered distribution.The utility model has the advantage that smaller sample spacing multichannel pipettor such as 8 channels or 12 channels pipettor sampling or sampling can be realized on the cell culture multi-well plate with larger single-hole area.

Description

Technical Field

[0001] This utility model belongs to the field of biological experimental equipment technology, and relates to a cell culture plate. Background Technology

[0002] Cell culture is a crucial step in cell biology and molecular biology experiments, and cell culture plates are the most commonly used containers for cell culture. Multi-well cell culture plates have a wide range of applications in biological experiments, such as 6-well, 12-well, 24-well, 48-well, 96-well, and 384-well plates. For 6-well to 48-well plates, due to the relatively small number of sample wells, users typically perform single-well sample addition or removal. For 96-well and 384-well plates, with the larger number of sample wells, they are often used for high-throughput experiments, and users generally use multi-channel pipettes or automated sample pipetting devices.

[0003] For example, a 96-well plate has an 8x12 well layout. There are two types of multichannel pipettes suitable for 96-well plates: 8-channel and 12-channel pipettes. The tip spacing of the 8-channel or 12-channel pipette is the same as the well spacing of a standard 96-well plate, which facilitates sample loading. In plates with larger well areas, such as 24-well plates with a 4x6 layout, the sample spacing of a standard 8-channel pipette is too small and unsuitable. Multi-well operations require unconventional multichannel pipettes, processing four or six samples per row at a time. This is less feasible due to limited availability of equipment and poor compatibility with other conventional multi-well plates; for example, it's difficult to sample samples from a 24-well plate into a 96-well plate. In some applications, we need larger well areas or volumes to accommodate larger volumes of samples for static or shaking cell culture, while also requiring multichannel pipettes, such as 8-channel or 12-channel pipettes, for rapid sample loading and unloading. In this situation, the single-well area or volume of a standard 96-well plate cannot meet the requirements, while 6-well to 48-well plates with larger single-well areas are not compatible with conventional multichannel pipettes with smaller sample spacing. Therefore, we need to improve existing cell culture plates so that they can be used with conventional 8-channel or 12-channel pipettes or unconventional multichannel pipettes with smaller sample spacing for sample addition or removal, while also having a larger single-well area or volume. Summary of the Invention

[0004] The purpose of this invention is to address the aforementioned problems in existing technologies by proposing a cell culture plate. The technical problem this invention aims to solve is how to implement multi-channel pipettes with smaller sample spacing, such as 8-channel or 12-channel pipettes, on a cell culture multi-well plate with a large single-well area for sample addition or removal.

[0005] The objective of this utility model can be achieved through the following technical solutions:

[0006] A cell culture plate includes a plate body having a plurality of culture wells arranged in rows and columns, characterized in that the plurality of culture wells are divided into multiple groups of culture wells in two columns, and the positions of two adjacent and parallel culture wells in each group of culture wells are staggered.

[0007] This cell culture plate uses a staggered arrangement of adjacent and parallel wells in a set of two columns. This ensures that all wells in each group have staggered sections along the column direction, and the line connecting these staggered sections lies on a straight line (a virtual line, not a physical line). Each well in the two columns is arranged staggered along this line, allowing the multi-channel pipette tips to simultaneously reach all wells in each group along the virtual line between the two columns. In other words, when the number of pipette tips in the multi-channel pipette is the same as the number of wells in each group, liquid can be transferred from all wells in each group in a single operation.

[0008] On the other hand, since the two parallel culture wells in each group are staggered, the volume of a single culture well on the cell culture plate can be maintained with minimal reduction, while the number of culture wells on the plate remains unchanged. This allows the cell culture plate to be better adapted to the operation of existing multichannel pipettes without reducing the number of culture wells or the volume of a single culture well. Furthermore, on cell culture multi-well plates with a larger single-well area, multichannel pipettes with smaller sample spacing can be used for sample addition or removal. Moreover, when the number of pipette tips in a row on the multichannel pipette is the same as the number of culture wells in each group, liquid can be transferred from all culture wells in each of the two columns of culture wells in a single operation, making the operation more convenient and efficient.

[0009] In the cell culture plate described above, each pair of parallel culture wells in each group of culture wells has a partition wall, and a portion of each partition wall protrudes toward one of the culture wells to form a groove one, while the other portion protrudes toward the other culture well to form a groove two.

[0010] The two ends of each partition wall of this cell culture plate are respectively recessed in opposite directions to form two staggered grooves along the column direction. This design ensures that grooves one and two on each group of culture wells are located on both sides of the same vertical plane (i.e., the middle position of the two columns of culture wells). The edges of grooves one and two are staggered along a straight line (a virtual straight line, not a physical straight line) at the middle position of the two columns of culture wells, making the shape of the culture wells on the plate more regular and easier to manufacture. At the same time, it is more conducive to the simultaneous insertion of the multi-channel pipette (device) tips into all the staggered grooves one and two in each group of culture wells along the straight line at the middle position of the two columns of culture wells. That is, when the number of pipette tips in the multi-channel pipette (device) is the same as the number of culture wells in each group of culture wells, the liquid transfer operation in each group of culture wells can be completed faster and better in one operation.

[0011] On the other hand, each culture well in each group of culture wells expands outward by the volume equivalent to groove one or groove two, while each culture well shrinks inward by the volume equivalent to groove two or groove one. This achieves the goal of not significantly reducing the volume of a single culture well on the cell culture plate, and better ensuring that the number of culture wells on the culture plate remains unchanged. Thus, without reducing the number of culture wells or the volume of a single culture well on the cell culture plate, the cell culture plate is better adapted to the operation of existing multichannel pipettes. This further enables the use of multichannel pipettes with smaller sample spacing for sample addition or removal on cell culture multi-well plates with larger single-well areas.

[0012] In the aforementioned cell culture plate, the first and second grooves protrude in opposite directions, and the spacer wall is S-shaped or reverse S-shaped. For example, the edges of the groove openings of the first and second grooves can be semi-circular, or similar to an arc or polygonal shape. The roughly S-shaped or reverse S-shaped spacer wall makes the shape of the spacer wall more regular, further facilitating the processing and manufacturing of the cell culture plate and ensuring that the volume of each culture well remains consistent.

[0013] In the aforementioned cell culture plate, the first and second grooves on the spacer wall are centrally symmetrically arranged. That is, in a group of two columns of several culture wells, adjacent wells in each column are centrally symmetrically arranged. This includes the shape of the wells, the first and second grooves on the spacer wall, and the bottom of the wells. This allows the volumes of two parallel wells in each group to better cancel each other out, resulting in consistent shapes within each column and central symmetry between columns. All the wells are essentially identical. This facilitates the manufacturing of the cell culture plate, and ensures consistent liquid oscillation during cell culture on a shaker, guaranteeing consistent experimental conditions across different wells.

[0014] In the aforementioned cell culture plate, the spacer walls in each group of culture wells have a wave-like structure, and the vertical walls of both the first and second convex grooves are semi-circular. The culture wells on the plate are arranged in a matrix, and the spacer walls in each group of culture wells are connected end-to-end along the column direction. This makes the shape of the culture wells more regular, the semi-circular vertical groove walls easier to manufacture, and also facilitates setting the four corners of the culture well walls to be arc-shaped.

[0015] In the aforementioned cell culture plate, the bottom wall of the culture well is conical. By making the bottom wall of the culture well conical, firstly, after cell culture with shaking, the entire culture plate can be centrifuged in a horizontal centrifuge, collecting the cells at the bottom of the cone for easy collection of supernatant or bottom cells; secondly, in mixed cell culture experiments, multiple cell types can aggregate in a small space under gravity, allowing for close contact and stimulation. It should be noted that the conical shape of the bottom wall refers to a gradually decreasing cross-section, not a strictly geometric cone. The conical edge in the vertical cross-section of the culture well can be a straight line or a curve; for example, a rounded bottom is also a type of cone shape.

[0016] In the aforementioned cell culture plate, the four corners of the well walls are all arc-shaped. By making the four corners of the well walls arc-shaped, it is convenient to add or remove samples using a multi-channel pipette, and the liquid flow in the wells is smoother when the cell culture plate is shaken in a shaker.

[0017] In the aforementioned cell culture plate, a cover plate is further included on the plate body. The outer edge of the plate body has an annular groove that surrounds a plurality of culture wells and is recessed downward. The edge of the cover plate has an annular edge portion that extends downward and an annular protrusion that is located inside the annular edge portion and protrudes downward. An annular recess is formed between the annular edge portion and the annular protrusion. The annular protrusion is embedded in the annular groove. The outer wall of the annular groove is embedded in the annular recess. The annular protrusion, the groove wall of the annular groove, and the wall of the annular recess together form a permeable channel that connects to the culture wells and is arranged in a tortuous manner. This cell culture plate forms a multi-twist ventilation channel connecting the culture wells and the ventilation gaps through the walls of the annular protrusions and annular grooves, as well as the walls of the annular protrusions and annular recesses. This multi-twist ventilation channel reduces airflow during air exchange between the outside and the culture wells inside the plate. Compared to ordinary multi-well plates, the gas exchange rate of this multi-twist ventilation channel is slower, thus better reducing or inhibiting the evaporation of moisture from the culture wells of the cell culture plate.

[0018] In the aforementioned cell culture plate, the outer edge of the plate has an annular stepped portion surrounding the annular groove. A ventilated gap is formed between the lower end face of the annular edge and the stepped surface of the annular stepped portion, connecting the ventilated channel to the outside. The small spacing of the ventilated gap reduces airflow during air exchange between the outside and the culture wells inside the plate. Furthermore, the multi-bend design of the ventilated channel slows down the gas exchange rate compared to ordinary multi-well plates, thus better reducing or inhibiting water evaporation from the culture wells and lowering the risk of cell contamination by external bacteria, fungi, etc.

[0019] In the aforementioned cell culture plate, the cover plate has several upwardly protruding recesses, each corresponding vertically to a culture well. The cross-sectional shape of the recesses matches the cross-sectional shape of the culture wells. The remaining portion of the cover plate forms a blocking portion that protrudes relative to the recesses. This prevents liquid from mixing within different culture wells, thus ensuring the reliability of the experimental results.

[0020] Compared with existing technologies, the advantages of this cell culture plate are as follows: 1. On the one hand, this cell culture plate allows the parallel pipette tips of existing multichannel pipettes to simultaneously extend into all the wells in each group of wells along a straight line between the two columns of wells. That is, when the number of parallel pipette tips on the multichannel pipette is the same as the number of wells in each group of wells, liquid can be transferred from all the wells in each group of wells in one operation. On the other hand, it ensures that the number of wells in each group of wells and the volume of each well remain basically unchanged compared to when the wells are distributed in a matrix. Thus, without reducing the number of wells on the cell culture plate and the volume of each well, the cell culture plate is better adapted to the operation of existing multichannel pipettes.

[0021] 2. This cell culture plate forms a tortuous air channel connecting to the culture wells through the plate body and the cover body. The lower end face of the annular edge abuts against the step surface of the annular step, and an air gap connecting the two forms the air channel, which allows for smooth gas exchange between the inside and outside of the cell culture plate, while also reducing the evaporation of water in the culture wells of the cell culture plate. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the three-dimensional structure of this cell culture plate.

[0023] Figure 2 This is a top view of the structure of this cell culture plate.

[0024] Figure 3 yes Figure 2 Sectional view at point AA.

[0025] Figure 4 yes Figure 3 A magnified view of a portion of point A in the middle.

[0026] Figure 5 This is a three-dimensional structural diagram of the cover plate of this cell culture plate.

[0027] Figure 6 This is a schematic diagram of the structure of the cover plate of this cell culture plate viewed from below.

[0028] Figure 7 This is a schematic diagram of the three-dimensional structure of the cell culture plate.

[0029] Figure 8 This is a top view of the structure of the cell culture plate.

[0030] Figure 9 This is a three-dimensional structural diagram of the cell culture plate viewed from below.

[0031] Figure 10This is a side view of the structure of the cell culture plate.

[0032] Figure 11 yes Figure 10 Sectional view at point BB.

[0033] Figure 12 This is a top view of the structure of the cell culture plate (with eight culture wells for operation).

[0034] Figure 13 This is a top view of the structure of the cell culture plate (with six culture wells for operation).

[0035] In the figure, 1. Plate body; 1a. Culture well; 1b. Annular groove; 1c. Annular stepped part; 1d. Spare wall; 1e. Vertical well wall; 2. Cover plate; 2a. Annular protrusion; 2b. Annular edge part; 2c. Annular recess; 2d. Recessed part; 2e. Blocking part; 3. Ventilation channel; 4. Groove one; 5. Groove two; 6. Ventilation gap; L - Straight line at the middle position of the two rows of culture wells. Detailed Implementation

[0036] The following are specific embodiments of the present invention, which are described in conjunction with the accompanying drawings. However, the present invention is not limited to these embodiments.

[0037] Example 1

[0038] A cell culture plate, as described Figure 1 , Figure 7 , Figure 8 and Figure 9 The plate body 1 includes a plurality of culture wells 1a arranged in rows and columns. The plurality of culture wells 1a are divided into multiple groups of culture wells 1a in pairs. The positions of two adjacent and parallel culture wells 1a in each group of culture wells 1a are staggered. Specifically, each pair of parallel culture wells 1a in each group of culture wells 1a has a partition wall 1d, and a portion of each partition wall 1d protrudes toward one culture well 1a to form a groove 4, while the other portion protrudes toward the other culture well 1a to form a groove 5. It should be noted that in this embodiment, the plurality of culture wells are divided into multiple groups of culture wells in pairs. In actual use, by rotating the culture plate by 90°, the "column" and "row" can be interchanged. From this perspective, the "column" and "row" are only different in name, but are essentially equivalent.

[0039] Furthermore, referring to Figure 1 , Figure 7 , Figure 8 and Figure 9The protrusion directions of the first groove 4 and the second groove 5 are opposite, and the partition wall 1d is "S" shaped or reverse "S" shaped; the first groove 4 and the second groove 5 on the partition wall 1d are centrally symmetrically arranged.

[0040] In this embodiment, it is preferred that the partition walls 1d in each group of culture wells 1a have a wave-like structure, and the vertical groove walls of the first groove 4 and the second groove 5 are both semi-circular. The culture wells 1a on the plate body 1 are arranged in a matrix, and the partition walls 1d in each group of culture wells 1a are connected end to end along the column direction. Preferably, the four corners of the culture well 1a wall are arc-shaped. The bottom wall of the culture well 1a is conical. Any two adjacent culture wells 1a arranged along the length direction of the plate body 1 also share a vertical hole wall 1e, and the vertical hole walls 1e arranged along the width direction of the plate body 1 are connected end to end.

[0041] In this embodiment, the bottom wall of the culture well 1a is preferably conical. After cell shaking culture, the entire culture plate can be centrifuged in a horizontal centrifuge, collecting the cells at the conical bottom for easy collection of supernatant or bottom cells. In cell co-culture experiments, this allows multiple cell types to aggregate in a small space under gravity, ensuring close contact and stimulation. The total number of culture wells 1a on plate 1 can be set differently depending on the required culture volume. For example... Figure 1 , Figure 7 , Figure 8 and Figure 9 The culture plate shown contains two sets of culture wells, each set containing 8 culture wells, for a total of 16 culture wells. Although each column of the culture plate has only 4 culture wells, the 8 culture wells in each set can be simultaneously added or removed by an 8-channel pipette through 8 staggered grooves 1 and 2 along the middle of the two columns of culture wells. The 8-channel pipette can also be used to transfer samples to the 96-well plate.

[0042] For example, refer to Figure 12 , Figure 13 The culture plates shown are those with 8 culture wells or those with 6 culture wells. Figure 12 and Figure 13 The text also briefly illustrates the operation of a multichannel pipette (device). For example, when a culture plate has 6 culture wells (refer to...). Figure 13 The two columns of culture wells shown in the figure are arranged in a column orientation (it should be noted that...). Figure 13Rotating 90° will arrange the two columns of culture wells in a row-oriented configuration (at this point, rows and columns are equivalent, only the names differ, and there is no substantial difference). A 6-channel pipette can simultaneously add or remove samples from six wells in two staggered grooves arranged in a straight line. Similarly, the above operation can be repeated multiple times to transfer samples from a standard 24-well (4x6-well) culture plate. Furthermore, while the dimensions of this cell culture plate are generally designed based on those of conventional culture plates, different dimensions can be used in actual use. For example, the volume of a single culture well can be increased by increasing the height of the culture plate, thus creating a deep-well plate.

[0043] Example 2

[0044] When ordinary multi-well plates, such as 6-well plates, 12-well plates, and similar plates, are used for shaking culture of cells in a shaker, the water evaporates quickly, which is not conducive to long-term cell culture. This embodiment addresses the above-mentioned background by improving existing multi-well plates to reduce the amount of water evaporation.

[0045] Specifically, a cell culture plate, as referenced Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 10 and Figure 11 It includes a plate body 1 with several culture wells 1a and a cover plate 2 covering the plate body 1. The cover plate 2 can be similar to the cover plate of a common multi-well plate, with a cover plate with an edge covering the plate body 1. In addition, the cover plate 2 and the plate body 1 can also use additional matching protrusions and grooves to improve the moisture retention and anti-pollution performance of the cover plate 2 and the plate body 1. Specifically, the outer edge of the plate body 1 has an annular groove 1b that surrounds a plurality of culture holes 1a and is recessed downward. The edge of the cover plate 2 has an annular edge portion 2b that extends downward and an annular protrusion 2a that is located inside the annular edge portion 2b and protrudes downward. An annular recess 2c is formed between the annular edge portion 2b and the annular protrusion 2a. The annular protrusion 2a is embedded in the annular groove 1b. The outer groove wall of the annular groove 1b is embedded in the annular recess 2c. The annular protrusion 2a, the groove wall of the annular groove 1b and the wall of the annular recess 2c together form a permeable channel 3 that connects to the culture holes 1a and is arranged in a tortuous manner.

[0046] In this embodiment, the outer edge of the plate 1 preferably has an annular step portion 1c surrounding the annular groove 1b. The lower end face of the annular edge portion 2b and the step surface of the annular step portion 1c form a ventilation channel 3 and a ventilation gap 6 connecting the ventilation channel 3 and the outside. Preferably, the distance between the inner top wall of the cover plate 2 and the top wall of the plate 1 is greater than the distance between the annular protrusion 2a and the groove wall of the annular groove 1b, so that the gas exchange inside and outside the cell culture plate is not hindered. It should be noted that the distance between the inner top wall of the cover plate 2 and the top wall of the plate 1, as well as the distance between the annular protrusion 2a and the groove wall of the annular groove 2c, can be formed by a small amount of irregular deformation during the plastic product manufacturing process. Alternatively, some small protrusions can be set on the top of the annular protrusion 2a or the bottom of the annular groove 2c so that the two cannot be completely fitted together, thus forming a gap. It is precisely because of the existence of the above-mentioned gap that the gas exchange inside and outside the cell culture plate can proceed smoothly.

[0047] Furthermore, referring to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 10 and Figure 11 The cover plate 2 has a plurality of upwardly protruding recesses 2d, the recesses 2d being vertically corresponding to the culture holes 1a, the cross-sectional shape of the recesses 2d matching the cross-sectional shape of the culture holes 1a, and the remaining parts of the cover plate 2 forming blocking parts 2e that protrude relative to the recesses 2d.

[0048] It should be noted that the contact between the plate 1 and the cover plate 2 only needs to have one point of contact. For example, a protrusion can be provided on the lower end face of the annular edge 2b or the step surface of the annular step 1c, so that the protrusion abuts against the lower end face of the annular edge 2b or the step surface of the annular step 1c, while the rest of the lower end face of the annular edge 2b and the step surface of the annular step 1c do not abut. Alternatively, a small amount of irregular deformation during the plastic product manufacturing process can naturally form a structure in which the lower end face of the annular edge 2b or the step surface of the annular step 1c abuts, thus forming a ventilation channel 3. Or, a gap can be left by designing the dimensions of the plate 1 and the cover plate 2 to form a ventilation channel 3.

[0049] Alternatively, depending on actual usage requirements, the annular step 1c may not be provided on the outside of the plate 1. That is, the annular edge 2b of the cover plate 2 covers the outside of the plate 1, and the ventilation channel 3 opens directly downwards. Without the annular step 1c, a protrusion can be provided along the path of the ventilation channel 3, so that the plate 1 and the cover plate 2 partially abut against each other without blocking the ventilation channel 3, thereby preserving breathability.

[0050] Example 3

[0051] A cell culture plate comprises a plate body 1 and a cover plate 2 as described in Examples 1 and 2. The cell culture plate described in this embodiment is generally made of a transparent material, such as polystyrene, and can be sterilized by irradiation or other methods when used for cell culture. The plate body 1 is generally produced by one-piece injection molding, but it can also be formed by an outer frame and multiple inserts, similar to some enzyme-linked immunosorbent assay (ELISA) multi-well plates. The two rows of insert culture wells can also be made into a culture plate conforming to the structure and application of this invention. The spacers between the culture wells can be solid or have hollow portions to reduce the probability of liquid flowing from one culture well to an adjacent culture well.

[0052] It should be noted that in this embodiment, the culture wells are divided into multiple groups of two columns each. In actual use, the "column" and "row" can be interchanged by rotating the culture plate by 90°. From this perspective, the "column" and "row" are equivalent. For example, when two columns of culture wells are grouped together, the first and second convex grooves are arranged along the width of the plate; while when two rows of culture wells are grouped together, the first and second convex grooves are arranged along the length. The culture plate described in this embodiment may contain one or more groups of culture wells (with two columns of culture wells as a group). In addition, the adjacent side of the first convex groove is a groove, and the adjacent side of the second convex groove is also a groove. For one side it is a convex groove, and for the other side it is a groove. From this perspective, the "convex groove" and "groove" are also equivalent.

[0053] Example 4

[0054] A cell culture plate includes a plate body 1 and a cover plate 2. In this embodiment, the culture wells distributed on the plate body 1 are ordinary round wells arranged in rows and columns. The bottom of the culture wells can be a plain flat bottom, a conical bottom, a U-shaped bottom, or a round bottom. The outer edge of the plate body 1 has a downwardly recessed annular groove 1b. The edge of the cover plate 2 has a downwardly extending annular edge portion 2b and an annular protrusion 2a located inside the annular edge portion 2b and protruding downward. An annular recess 2c is formed between the annular edge portion 2b and the annular protrusion 2a. The annular protrusion 2a is embedded in the annular groove 1b, and the outer wall of the annular groove 1b is embedded in the annular recess 2c. The annular protrusion 2a, the groove wall of the annular groove 1b, and the wall of the annular recess 2c together form a tortuous air passage 3 that connects to the culture wells 1a. In this embodiment, the outer edge of the plate 1 preferably has an annular step portion 1c surrounding the annular groove 1b, and a ventilation channel 3 and a ventilation gap 6 connecting the lower end face of the annular edge portion 2b and the step surface of the annular step portion 1c are formed.

[0055] The specific embodiments described herein are merely illustrative examples illustrating the spirit of this utility model. Those skilled in the art to which this utility model pertains may make various modifications or additions to the described specific embodiments or use similar methods to substitute them, without departing from the spirit of this utility model or exceeding the scope defined by the appended claims.

Claims

1. A cell culture plate comprising a plate body (1) having a number of culture wells (1a) arranged in rows and columns, characterized in that The culture wells (1a) are divided into multiple groups of culture wells (1a) in two columns, and the positions of two adjacent and parallel culture wells (1a) in each group of culture wells (1a) are staggered.

2. The cell culture plate of claim 1, wherein, Each pair of culture wells (1a) in each group of culture wells (1a) has a partition wall (1d), and a portion of each partition wall (1d) protrudes toward one of the culture wells (1a) to form a first groove (4), and the other portion protrudes toward the other culture well (1a) to form a second groove (5).

3. The cell culture plate of claim 2, wherein, The protrusion directions of the first groove (4) and the second groove (5) are opposite, and the partition wall (1d) is "S" shaped or reverse "S" shaped.

4. The cell culture plate of claim 3, wherein, The first groove (4) and the second groove (5) on the partition wall (1d) are arranged in a centrally symmetrical manner.

5. A cell culture plate according to claim 4, wherein, The partition walls (1d) in each group of culture wells (1a) are in a wave-like structure, and the vertical groove walls of the first groove (4) and the second groove (5) are both semi-circular. The culture wells (1a) on the plate (1) are distributed in a matrix, and the partition walls (1d) in each group of culture wells (1a) are connected end to end along the column direction.

6. A cell culture plate according to claim 1, characterized in that, The bottom wall of the culture well (1a) is conical.

7. The cell culture plate of claim 1, wherein, The four corners of the culture well (1a) are all arc-shaped.

8. The cell culture plate according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7, wherein, It also includes a cover plate (2) covering the plate body (1). The outer edge of the plate body (1) has an annular groove (1b) that surrounds a plurality of culture holes (1a) and is recessed downward. The edge of the cover plate (2) has an annular edge portion (2b) that extends downward and an annular protrusion (2a) that is located inside the annular edge portion (2b) and protrudes downward. An annular recess (2c) is formed between the annular edge portion (2b) and the annular protrusion (2a). The annular protrusion (2a) is embedded in the annular groove (1b). The outer wall of the annular groove (1b) is embedded in the annular recess (2c). The annular protrusion (2a), the groove wall of the annular groove (1b), and the wall of the annular recess (2c) together form a permeable channel (3) that connects to the culture hole (1a) and is arranged in a tortuous manner.

9. A cell culture plate according to claim 8, wherein, The outer edge of the plate (1) has an annular step portion (1c) surrounding the annular groove (1b), and a ventilation gap (6) connecting the ventilation channel (3) and the outside is formed between the lower end face of the annular edge portion (2b) and the step surface of the annular step portion (1c).

10. The cell culture plate of claim 8, wherein, The cover plate (2) has a plurality of upwardly protruding recesses (2d), the recesses (2d) and the culture holes (1a) are positioned vertically to each other, the cross-sectional shape of the recesses (2d) matches the cross-sectional shape of the culture holes (1a), and the remaining parts of the cover plate (2) form blocking parts (2e) that protrude relative to the recesses (2d).

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