An automatic counting device for a cell counter

Abstract

This utility model discloses an automatic cell counting device, relating to the field of biomedical engineering technology. It includes a base with a metering box fixedly connected to the left side of the top of the base. The automatic cell counting device is equipped with a polycarbonate filter membrane, a superhydrophilic coating, and a Venturi tube. In use, a mucus sample is placed on the polycarbonate filter membrane, first contacting the superhydrophilic coating to prevent the adsorption of mucin in the mucus. The sample then flows downwards into the polycarbonate filter membrane, where the mucus is filtered to obtain a single-cell suspension. This suspension flows downwards into the Venturi tube, creating a micro-negative pressure environment as the cells pass through, preventing mechanical shearing forces from damaging the cells. The filtered cells then fall into a collection tray, which is placed on a detection pool. The sample is then imaged using a microscope imaging head, and the images are automatically identified and counted using image processing algorithms. This addresses the problem of devices being unable to handle viscous samples.

Description

Technical Field

[0001] This utility model relates to the field of biomedical engineering technology, specifically to an automatic counting device for a cell counter. Background Technology

[0002] Biomedical engineering technology mainly develops technical equipment for disease diagnosis, treatment monitoring, and life science research. Among them, cell counters use optical imaging such as microscopes, flow cytometry, and intelligent algorithm image recognition and data analysis to achieve automated detection of parameters such as cell number, morphology, and viability. They are widely used in drug development, cancer research, and clinical diagnosis. However, because the samples being tested are different and the quality of single-cell suspensions varies greatly, most current automated cell counters cannot directly count viscous samples.

[0003] Now, a novel automatic cell counter device is proposed to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide an automatic counting device for cell counters to solve the problem mentioned in the background art of being unable to handle viscous samples.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an automatic counting device for a cell counter, comprising a base, a measuring box fixedly connected to the left side of the top of the base, and a mucus processing component fixedly connected to the left side inside the measuring box.

[0006] The mucus treatment assembly includes a support frame fixedly connected to the left side inside the metering chamber. A polycarbonate filter membrane is fixedly connected inside the support frame, and a superhydrophilic coating is fixedly connected to the top of the polycarbonate filter membrane. Multiple sets of Venturi tubes are fixedly connected inside the polycarbonate filter membrane. A receiving tray is provided below the polycarbonate filter membrane. A platform is fixedly connected to the middle position inside the metering chamber, and a detection pool is fixedly connected to the top of the platform. A microscope imaging head is fixedly connected to the top of the metering chamber.

[0007] As a further technical solution of this utility model, the Venturi tubes are arranged at equal intervals, and there is a distance between the polycarbonate filter membrane and the receiving tray.

[0008] As a further technical solution of this utility model, the external shape and size of the receiving plate are consistent with the internal shape and size of the bracket, and the receiving plate can be moved back and forth along the inside of the bracket.

[0009] As a further technical solution of this utility model, the microscope imaging head is aligned with the vertical center line of the detection cell, and the support is connected to the platform.

[0010] As a further technical solution of this utility model, ultraviolet lamps are provided on the left and right sides of the microscope imaging head, a solution tank is fixedly connected inside the platform, a peristaltic pump is provided on the right side of the solution tank, a pull tube is fixedly connected to the front end of the peristaltic pump, and multiple sets of heating tubes are fixedly connected to the left side of the solution tank.

[0011] As a further technical solution of this utility model, the ultraviolet lamp is fixedly connected to the metering box, the peristaltic pump is fixedly connected to the platform, the peristaltic pump is connected to the interior of the solution tank, the peristaltic pump is connected to the interior of the pull tube, and the pull tube is elastic.

[0012] As a further technical solution of this utility model, a miniature water pump is fixedly connected to the right side of the bottom of the metering box, an inlet pipe is fixedly connected to the left side of the miniature water pump, a liquid collection tank is provided on the right side of the metering box, a controller is fixedly connected to the top of the liquid collection tank, and a level gauge is fixedly connected to the bottom of the controller.

[0013] As a further technical solution of this utility model, the micro water pump is connected to the inside of the water inlet pipe, the micro water pump is connected to the inside of the liquid collection tank, the liquid level gauge passes through the liquid collection tank and extends into the inside of the liquid collection tank, and the liquid collection tank is fixedly connected to the base.

[0014] Compared with the prior art, the beneficial effects of this utility model are: the automatic counting device of the cell counter not only realizes the function of mucus pretreatment, but also realizes the function of biological residue decomposition and waste liquid collection.

[0015] (1) By setting up a polycarbonate filter membrane, a superhydrophilic coating and a Venturi tube, when in use, the mucus sample is placed on the polycarbonate filter membrane and first comes into contact with the superhydrophilic coating. The superhydrophilic coating prevents the adsorption of mucin in the mucus. Then it enters the polycarbonate filter membrane to filter the mucus and obtain a single-cell suspension. The single-cell suspension flows downward into the Venturi tube, and a micro-negative pressure environment is generated when the cells pass through to avoid mechanical shear force causing cell damage. Then the filtered cells fall downward into the collection tray for collection. The collection tray is then placed on the detection pool and photographed by the microscope imaging head. Then the image processing algorithm is used to automatically identify and count the cells, thus realizing the mucus pretreatment function.

[0016] (2) By setting up a solution tank and a heating tube, when in use, after the cell counting is completed, the ultraviolet lamp is turned on. The UVC band of the light emitted by the ultraviolet lamp can destroy the DNA structure of microorganisms and achieve a high sterilization rate. After sterilization, the heating tube can be turned on to heat the compound enzyme solution in the solution tank to a certain degree. Then, the compound enzyme solution is drawn from the solution tank to the pull tube and discharged from the pull tube to the detection pool to enzymatically decompose biological residues, thus realizing the function of decomposing biological residues.

[0017] (3) With a collection tank and a level gauge, after sterilization and decomposition are completed, a micro water pump can be started to extract the remaining waste liquid on the test pool through the water inlet pipe. The waste liquid is then drawn into the collection tank and sealed for storage. At the same time, the level gauge detects the liquid volume inside. When the internal liquid level reaches the preset value, the controller issues an automatic warning to prompt the staff to treat the waste liquid, thus realizing the waste liquid collection function. Attached Figure Description

[0018] Figure 1 This is a front view structural diagram of the present utility model;

[0019] Figure 2 This is an enlarged front cross-sectional view of the present invention.

[0020] Figure 3 This is a magnified cross-sectional view of the polycarbonate filter membrane of this utility model.

[0021] Figure 4 This is an enlarged cross-sectional view of the solution tank of this utility model.

[0022] In the diagram: 1. Base; 2. Metering box; 3. Support; 4. Polycarbonate filter membrane; 5. Superhydrophilic coating; 6. Venturi tube; 7. Receiving tray; 8. Platform; 9. Detection cell; 10. Microscope imaging head; 11. Ultraviolet lamp; 12. Solution tank; 13. Peristaltic pump; 14. Pull-out tube; 15. Heating element; 16. Miniature water pump; 17. Inlet pipe; 18. Collection tank; 19. Controller; 20. Level gauge. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figure 1-4 An embodiment of this utility model is provided: an automatic counting device for a cell counter, including a base 1, a measuring box 2 fixedly connected to the left side of the top of the base 1, and a mucus treatment component fixedly connected to the left side inside the measuring box 2.

[0025] Please see Figure 1-4An automatic cell counter device also includes a mucus treatment component. The mucus treatment component includes a support 3, which is fixedly connected to the left side inside the metering chamber 2. A polycarbonate filter membrane 4 is fixedly connected inside the support 3. A superhydrophilic coating 5 is fixedly connected to the top of the polycarbonate filter membrane 4. Multiple sets of Venturi tubes 6 are fixedly connected inside the polycarbonate filter membrane 4. A receiving tray 7 is provided below the polycarbonate filter membrane 4. A platform 8 is fixedly connected to the middle position inside the metering chamber 2. A detection cell 9 is fixedly connected to the top of the platform 8. A microscope imaging head 10 is fixedly connected to the top of the metering chamber 2. The Venturi tubes 6 are arranged at equal intervals. There is a distance between the polycarbonate filter membrane 4 and the receiving tray 7. The external shape and size of the receiving tray 7 are consistent with the internal shape and size of the support 3. The receiving tray 7 can be moved back and forth along the inside of the support 3. The microscope imaging head 10 is aligned with the vertical center line of the detection cell 9. The support 3 is connected to the platform 8 to facilitate the pretreatment of mucus.

[0026] Specifically, such as Figure 1 , Figure 2 and Figure 3 As shown, during use, the mucus sample is placed on the polycarbonate filter membrane 4, first contacting the superhydrophilic coating 5. The superhydrophilic coating 5 prevents the adsorption of mucin in the mucus, and then it flows downward into the polycarbonate filter membrane 4 to filter the mucus, obtaining a single-cell suspension. The single-cell suspension flows downward into the Venturi channel 6, creating a micro-negative pressure environment as the cells pass through, avoiding mechanical shear force that could damage the cells. After filtration, the cells fall downward into the collection tray 7 for collection. The collection tray 7 is then placed on the detection pool 9, and the sample is photographed using the microscope imaging head 10. Subsequently, the image processing algorithm is used to automatically identify and count the cells, facilitating the detection of the mucus sample.

[0027] Ultraviolet lamps 11 are provided on the left and right sides of the microscope imaging head 10. A solution tank 12 is fixedly connected inside the platform 8. A peristaltic pump 13 is provided on the right side of the solution tank 12. A pull tube 14 is fixedly connected to the front end of the peristaltic pump 13. Multiple heating tubes 15 are fixedly connected to the left side of the solution tank 12. The ultraviolet lamps 11 are fixedly connected to the metering box 2. The peristaltic pump 13 is fixedly connected to the platform 8. The peristaltic pump 13 is connected to the inside of the solution tank 12. The peristaltic pump 13 is connected to the inside of the pull tube 14. The pull tube 14 is elastic and convenient for cleaning the inside.

[0028] Specifically, such as Figure 1 and Figure 4As shown, during use, after the cell counting is completed, the ultraviolet lamp 11 is turned on. The UVC band of the light emitted by the ultraviolet lamp 11 can destroy the DNA structure of microorganisms, achieving a high sterilization rate. After sterilization, the heating tube 15 can be turned on to heat the compound enzyme solution in the solution tank 12 to 37 degrees. Then, the compound enzyme solution is drawn from the solution tank 12 to the pull tube 14, and then discharged from the pull tube 14 to the detection pool 9 to enzymatically decompose biological residues and keep the inside of the equipment clean.

[0029] A micro water pump 16 is fixedly connected to the right side of the bottom of the metering box 2. A water inlet pipe 17 is fixedly connected to the left side of the micro water pump 16. A liquid collection tank 18 is set on the right side of the metering box 2. A controller 19 is fixedly connected to the top of the liquid collection tank 18. A liquid level gauge 20 is fixedly connected to the bottom of the controller 19. The micro water pump 16 is connected to the inside of the water inlet pipe 17 and the inside of the liquid collection tank 18. The liquid level gauge 20 passes through the liquid collection tank 18 and extends into the inside of the liquid collection tank 18. The liquid collection tank 18 is fixedly connected to the base 1 for easy collection of waste liquid.

[0030] Specifically, such as Figure 1 and Figure 2 As shown, during use, after sterilization and decomposition are completed, the micro water pump 16 can be started to extract the remaining waste liquid on the detection pool 9 through the water inlet pipe 17, and the waste liquid is drawn into the collection tank 18 for sealed storage. At the same time, the liquid level gauge 20 detects the liquid level inside. When the liquid level inside reaches the preset value, the controller 19 issues an automatic warning to prompt the staff to treat the waste liquid, which facilitates the management of the waste liquid.

[0031] Working Principle: In use, the mucus sample is first placed on the polycarbonate filter membrane 4, initially contacting the superhydrophilic coating 5. This coating prevents the adsorption of mucin proteins in the mucus. The sample then flows downwards into the polycarbonate filter membrane 4, where it is filtered to obtain a single-cell suspension. This suspension flows downwards into the Venturi channel 6, creating a slightly negative pressure environment as the cells pass through, preventing mechanical shearing damage. The filtered cells then fall into the collection tray 7 for collection. The collection tray 7 is then placed on the detection pool 9, and the sample is imaged using a microscope imaging head 10. The image processing algorithm then automatically identifies and counts the cells. After cell counting is complete, the ultraviolet lamp 11 is activated. The UVC band of light emitted by the external lamp tube 11 can destroy the DNA structure of microorganisms, achieving a high sterilization rate. After sterilization, the heating tube 15 can be activated to heat the compound enzyme solution in the solution tank 12 to 37 degrees Celsius. Then, the compound enzyme solution is drawn from the solution tank 12 to the pull tube 14 and discharged from the pull tube 14 to the detection pool 9 to enzymatically decompose biological residues. When in use, after sterilization and decomposition, the micro water pump 16 can be activated to draw the remaining waste liquid on the detection pool 9 through the water inlet pipe 17 and collect the waste liquid into the collection tank 18 for sealed storage. At the same time, the liquid level gauge 20 detects the liquid level inside. When the liquid level inside reaches the preset value, the controller 19 issues an automatic warning to prompt the staff to treat the waste liquid.

[0032] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A cell counter automated counting device comprising a base (1) characterised in that: A metering box (2) is fixedly connected to the left side of the top of the base (1), and a mucus treatment component is fixedly connected to the left side inside the metering box (2). The mucus treatment assembly includes a support (3), which is fixedly connected to the left side inside the metering box (2). A polycarbonate filter membrane (4) is fixedly connected inside the support (3). A superhydrophilic coating (5) is fixedly connected to the top of the polycarbonate filter membrane (4). Multiple sets of Venturi tubes (6) are fixedly connected inside the polycarbonate filter membrane (4). A receiving tray (7) is provided below the polycarbonate filter membrane (4). A platform (8) is fixedly connected to the middle position inside the metering box (2). A detection pool (9) is fixedly connected to the top of the platform (8). A microscope imaging head (10) is fixedly connected to the top inside the metering box (2).

2. The automatic counting device of a cell counter according to claim 1, wherein: The Venturi tubes (6) are arranged at equal intervals, and there is a distance between the polycarbonate filter membrane (4) and the receiving plate (7).

3. The automatic counting device of a cell counter according to claim 1, wherein: The external shape and size of the receiving plate (7) are consistent with the internal shape and size of the bracket (3), and the receiving plate (7) can be moved back and forth along the inside of the bracket (3).

4. The automatic counting device of a cell counter according to claim 1, wherein: The microscope imaging head (10) is aligned with the vertical center line of the detection cell (9), and the support (3) is connected to the platform (8).

5. The automatic counting device of a cell counter according to claim 1, wherein: Ultraviolet lamps (11) are provided on the left and right sides of the microscope imaging head (10). A solution tank (12) is fixedly connected inside the platform (8). A peristaltic pump (13) is provided on the right side of the solution tank (12). A pull tube (14) is fixedly connected to the front end of the peristaltic pump (13). Multiple sets of heating tubes (15) are fixedly connected to the left side of the solution tank (12).

6. The automatic counting device of a cell counter according to claim 5, wherein: The ultraviolet lamp (11) is fixedly connected to the metering box (2), the peristaltic pump (13) is fixedly connected to the platform (8), the peristaltic pump (13) is connected to the interior of the solution tank (12), the peristaltic pump (13) is connected to the interior of the pull tube (14), and the pull tube (14) is elastic.

7. The automatic counting device of a cell counter according to claim 1, wherein: A micro water pump (16) is fixedly connected to the right side of the bottom of the metering box (2). A water inlet pipe (17) is fixedly connected to the left side of the micro water pump (16). A liquid collection tank (18) is provided on the right side of the metering box (2). A controller (19) is fixedly connected to the top of the liquid collection tank (18). A level gauge (20) is fixedly connected to the bottom of the controller (19).

8. The automatic counting device of a cell counter according to claim 7, wherein: The micro water pump (16) is connected to the inside of the water inlet pipe (17), the micro water pump (16) is connected to the inside of the liquid collection tank (18), the liquid level gauge (20) passes through the liquid collection tank (18) and extends into the inside of the liquid collection tank (18), and the liquid collection tank (18) is fixedly connected to the base (1).

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