A microfluidic chip device for pretreatment of oral swab nucleic acid detection

Abstract

The utility model relates to biological detection technical field, concretely relates to a kind of for oral swab nucleic acid detection pretreatment microfluidic chip device;Including chip main body, the chip main body is equipped with sequentially communicating liquid addition hole, sample loading area, S type microfluidic flow guide channel, sample release freeze-drying reagent hole, release finished sample chamber, PCR freeze-drying reagent hole, metering chamber, microfluidic capillary valve and fluorescence detection chamber;Between S type microfluidic flow guide channel and sample release freeze-drying reagent hole, between sample release freeze-drying reagent hole and release finished sample chamber, between release finished sample chamber and PCR freeze-drying reagent hole and between PCR freeze-drying reagent hole and metering chamber respectively are equipped with vortex mixing unit;The utility model integrates multiple functional areas on chip main body, simplifies the detection process of oral swab sample, reduces intermediate links such as sample transfer, reduces the pollution risk to oral swab sample, improves the accuracy of detection result.

Description

Technical Field

[0001] This utility model relates to the field of biological detection technology, specifically to a microfluidic chip device for preprocessing nucleic acid detection of oral swabs. Background Technology

[0002] In existing nucleic acid detection technologies, the processing and detection of oral swab samples is relatively complex, often requiring multiple independent devices and steps, including sample transfer, lysis, nucleic acid extraction, and PCR amplification. This not only makes the detection process cumbersome and time-consuming, but also easily introduces contamination during sample transfer and other stages, affecting the accuracy of the test results. Therefore, to solve the above technical problems, this application proposes a microfluidic chip device for the preprocessing of oral swab nucleic acid detection. Utility Model Content

[0003] The purpose of this invention is to provide a microfluidic chip device for pretreatment of oral swab nucleic acid detection, which aims to solve the problems of complex oral swab nucleic acid detection process, susceptibility to contamination, and difficulty in achieving rapid on-site detection in the prior art.

[0004] To achieve the above objectives, the technical solution of this utility model is as follows:

[0005] This invention provides a microfluidic chip device for pretreatment of oral swabs for nucleic acid detection, comprising a chip body 1, wherein the chip body is provided with a liquid inlet 2, a sample loading area 3, an S-shaped microfluidic flow channel 4, a sample release lyophilized reagent well 5, a sample release chamber 6, a PCR lyophilized reagent well 7, a metering chamber 8, a microfluidic capillary valve 9, a fluorescence detection chamber 10, and several vortex mixing units. The liquid inlet 2, sample loading area 3, S-shaped microfluidic flow channel 4, sample release lyophilized reagent well 5, sample release chamber 6, PCR lyophilized reagent well 7, metering chamber 8, microfluidic capillary valve 9, and fluorescence detection chamber 10 are sequentially connected. The plurality of vortex mixing units include vortex mixing unit I 11-1 located between the S-shaped microfluidic guide channel 4 and the sample release lyophilized reagent well 5; vortex mixing unit II 11-2 located between the sample release lyophilized reagent well 5 and the released sample chamber 6; vortex mixing unit III 11-3 located between the released sample chamber 6 and the PCR lyophilized reagent well 7; and vortex mixing unit IV 11-4 located between the PCR lyophilized reagent well 7 and the metering chamber 8. Each vortex mixing unit includes a circular vortex generator used to generate turbulent shear force to rehydrate the lyophilized beads or lyophilized reagents.

[0006] Furthermore, the S-shaped microfluidic channel 4 is designed to ensure that the sample solution can be stably and uniformly delivered into the sample release lyophilized reagent well 5.

[0007] Furthermore, the sample release lyophilization reagent well 5 is provided with lyophilized beads containing lysis buffer and surfactant, which are used to lyse oral epithelial cells and release nucleic acid target fragments.

[0008] Furthermore, the PCR lyophilized reagent well 7 is equipped with a lyophilized enzyme system containing fluorescent probes for PCR amplification and detection of nucleic acid samples.

[0009] Furthermore, the microfluidic capillary valve 9 is used to control the amount of nucleic acid sample dispensed, accurately dispensing the nucleic acid sample into the fluorescence detection chamber 10.

[0010] The beneficial effects achieved by this utility model are as follows:

[0011] This invention integrates the sample loading area, S-shaped microfluidic flow channel, sample release lyophilized reagent well, PCR lyophilized reagent well, microfluidic capillary valve, and fluorescence detection chamber onto the chip body, forming a complete microfluidic system for pretreatment of oral swab nucleic acid detection. This simplifies the detection process, reduces intermediate steps such as sample transfer, lowers the risk of contamination, and improves the accuracy of detection results.

[0012] The circular vortex generator of this invention's vortex mixing unit can generate turbulent shear force, which promotes the full reconstitution of the lyophilized beads, ensuring the effectiveness of sample lysis and nucleic acid release, as well as the full reconstitution of PCR lyophilized reagents, providing a reliable guarantee for subsequent nucleic acid detection.

[0013] This novel microfluidic capillary valve can precisely control the distribution of nucleic acid samples. Combined with a real-time fluorescence signal acquisition system, it enables rapid and accurate qualitative analysis of nucleic acids. Furthermore, the device is compact, portable, and easy to operate, meeting the needs of rapid on-site testing and possessing broad application prospects. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the working principle of this utility model.

[0016] In the diagram, 1. Chip body; 2. Liquid filling port; 3. Sample loading area; 4. S-shaped microfluidic flow channel; 5. Sample release lyophilized reagent port; 6. Sample release chamber; 7. PCR lyophilized reagent port; 8. Measurement chamber; 9. Microfluidic capillary valve; 10. Fluorescence detection chamber; 11-1. Vortex mixing unit I; 11-2. Vortex mixing unit II; 11-3. Vortex mixing unit III; 11-14. Vortex mixing unit IV. Detailed Implementation

[0017] The technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0018] It should be noted that if the embodiments of this utility model involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0019] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, if the word "and / or" appears throughout the text, it means including three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution that simultaneously satisfies A and B. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0020] like Figure 1As shown, this utility model provides a microfluidic chip device for pretreatment of oral swab nucleic acid detection, including a chip body 1. The chip body is provided with a liquid inlet 2, a sample loading area 3, an S-shaped microfluidic flow channel 4, a sample release lyophilized reagent well 5, a sample release chamber 6, a PCR lyophilized reagent well 7, a metering chamber 8, a microfluidic capillary valve 9, a fluorescence detection chamber 10, and several vortex mixing units. The liquid inlet 2, sample loading area 3, S-shaped microfluidic flow channel 4, sample release lyophilized reagent well 5, sample release chamber 6, PCR lyophilized reagent well 7, metering chamber 8, microfluidic capillary valve 9, and fluorescence detection chamber 10 are sequentially connected. The plurality of vortex mixing units include vortex mixing unit I11-1 located between the S-shaped microfluidic channel 4 and the sample release lyophilized reagent well 5; vortex mixing unit II11-2 located between the sample release lyophilized reagent well 5 and the released sample chamber 6; vortex mixing unit III11-3 located between the released sample chamber 6 and the PCR lyophilized reagent well 7; and vortex mixing unit IV11-4 located between the PCR lyophilized reagent well 7 and the metering chamber 8. The S-shaped microfluidic channel 4 is designed to ensure that the sample solution can be stably and uniformly delivered into the sample release lyophilized reagent well 5. The sample release lyophilized reagent well 5 contains lyophilized beads containing lysis buffer and surfactant, used to lyse oral epithelial cells and release nucleic acid target fragments. The PCR lyophilized reagent well 7 contains a lyophilized enzyme system containing fluorescent probes, used for PCR amplification and detection of nucleic acid samples. The microfluidic capillary valve 9 is used to control the amount of nucleic acid sample dispensed, accurately dispensing the nucleic acid sample into the fluorescence detection chamber 10. The vortex mixing unit 11 includes a circular vortex generator, used to generate turbulent shear force to rehydrate the lyophilized beads or lyophilized reagents.

[0021] The working principle of this utility model is as follows:

[0022] First, the oral swab sample solution is added to the sample loading area 3 of the chip body 1 through the liquid filling hole 2. Under the action of gravity or other driving force, the sample solution flows along the S-shaped microfluidic guide channel 4. Since the length and shape of the S-shaped microfluidic guide channel are optimized, the sample solution can be stably and uniformly delivered to the sample release lyophilized reagent well 5.

[0023] When the sample solution reaches the sample release lyophilized reagent well 5, it comes into contact with the lyophilized beads inside the well. At this point, the circular vortex generators of vortex mixing unit I 11-1 and vortex mixing unit II 11-2 begin to operate, generating turbulent shear force to promote the complete resolution of the lyophilized beads (containing lysis buffer and surfactant). Under the action of the lysis buffer and surfactant, the oral epithelial cells lyse and release the nucleic acid target fragments.

[0024] After release, the nucleic acid sample, propelled by turbulent shear force, enters the PCR lyophilization reagent well 7 through the released sample chamber 6. The lyophilized enzyme system containing the fluorescent probe in the PCR lyophilization reagent well 7 is then fully reconstituted by vortex mixing units III 11-3 and IV 11-4 under the influence of the nucleic acid sample. The reconstituted mixture is then precisely dispensed into the fluorescence detection chamber 10 via a microfluidic capillary valve 9. A real-time fluorescence signal acquisition system (not shown in the figure) corresponds to the fluorescence detection chamber 10 and acquires real-time fluorescence signals from the nucleic acid sample within 10. Based on the acquired fluorescence signals, qualitative analysis of the nucleic acid is performed using appropriate analysis algorithms and software, thus obtaining the detection results.

[0025] The above description is only an optional embodiment of the present utility model and does not limit the patent scope of the present utility model. All equivalent structural transformations made under the inventive concept of the present utility model using the contents of the present utility model specification and drawings, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present utility model.

Claims

1. A microfluidic chip device for preprocessing nucleic acid detection in oral swabs, characterized in that: The chip body includes a liquid filling hole (2), a sample loading area (3), an S-shaped microfluidic flow channel (4), a sample release lyophilized reagent hole (5), a released sample chamber (6), a PCR lyophilized reagent hole (7), a metering chamber (8), a microfluidic capillary valve (9), a fluorescence detection chamber (10), and several vortex mixing units. The liquid filling hole (2), sample loading area (3), S-shaped microfluidic flow channel (4), sample release lyophilized reagent hole (5), released sample chamber (6), PCR lyophilized reagent hole (7), metering chamber (8), microfluidic capillary valve (9), and fluorescence detection chamber (10) are connected in sequence. The plurality of vortex mixing units include vortex mixing unit I (11-1) located between the S-shaped microfluidic guide channel (4) and the sample release lyophilized reagent well (5), vortex mixing unit II (11-2) located between the sample release lyophilized reagent well (5) and the released sample chamber (6), vortex mixing unit III (11-3) located between the released sample chamber (6) and the PCR lyophilized reagent well (7), and vortex mixing unit IV (11-4) located between the PCR lyophilized reagent well (7) and the metering chamber (8).

2. The microfluidic chip device for preprocessing oral swab nucleic acid detection according to claim 1, characterized in that: The sample release lyophilized reagent well (5) contains lyophilized beads containing lysis buffer and surfactant, which are used to lyse oral epithelial cells and release nucleic acid target fragments.

3. The microfluidic chip device for preprocessing oral swab nucleic acid detection according to claim 1, characterized in that: The PCR lyophilized reagent well (7) contains a lyophilized enzyme system with fluorescent probes, which is used for PCR amplification and detection of nucleic acid samples.

4. The microfluidic chip device for preprocessing oral swab nucleic acid detection according to claim 1, characterized in that: The microfluidic capillary valve (9) is used to control the amount of nucleic acid sample dispensed, accurately dispensing the nucleic acid sample into the fluorescence detection chamber (10).

5. A microfluidic chip device for preprocessing oral swab nucleic acid detection according to claim 1, characterized in that: The vortex mixing unit (11) includes a circular vortex generator for generating turbulent shear force to reconstitute the lyophilized beads or lyophilized reagents.

Images