A microbial outer membrane vesicle preparation device

Abstract

The utility model relates to the field of medicine preparation discloses a kind of microbial outer membrane vesicle preparation devices, including high-pressure homogenizer main part and centrifuge, and high-pressure homogenizer main part top one end is provided with culture piece, and high-pressure homogenizer main part top other end is provided with air passage piece;Culture piece includes the culture cylinder of detachable installation in high-pressure homogenizer main part top, and culture cylinder top is threadedly connected with cover, and cover top detachably installs motor, and motor output end is detachably installed with stirring rod through coupling, and stirring rod penetrates cover and is inserted in culture cylinder, and culture cylinder side wall is fixedly installed with first water pump, and high-pressure homogenizer main part side is fixedly installed with first connecting sleeve, and first connecting sleeve and first water pump between detachably installed with first connecting pipe, and high-pressure homogenizer main part detachably installs pressure gauge;The utility model solves the problem of the yield fluctuation of outer membrane vesicle by culture piece in the preparation process of microbial outer membrane vesicle, and the distribution of external environment and culture solution is influenced.

Description

Technical Field

[0001] This utility model relates to the field of pharmaceutical preparation, specifically to a device for preparing microbial outer membrane vesicles. Background Technology

[0002] Microbial outer membrane vesicles are nanoscale vesicles actively secreted by bacteria and other microorganisms. They contain various bioactive molecules such as proteins, nucleic acids, and lipids, and play important roles in intercellular communication, antigen delivery, and immune regulation. Currently, the preparation of microbial outer membrane vesicles is of great significance for in-depth research into their functions and the development of related diagnostic and therapeutic methods.

[0003] A high-pressure homogenizer is a device that uses high pressure to refine, emulsify, disperse, and homogenize materials. It is widely used in the food, pharmaceutical, chemical, and cosmetic industries. Its core principle is to place the material in a high-pressure environment, causing it to undergo intense shearing, impact, and cavitation within a specific homogenizing valve. This breaks down particles or droplets in the material, achieving uniform mixing and refining.

[0004] Existing microbial outer membrane vesicle preparation devices have the following drawbacks: the culture device and the high-pressure homogenizer are independent equipment, the microbial culture medium needs to be manually transferred to the high-pressure homogenizer, and it is easily contaminated by the outside world during the transfer process. In addition, there is no stable stirring mechanism during the culture process, the microorganisms are unevenly distributed in the culture medium, which affects their growth status and leads to fluctuations in the yield of outer membrane vesicles. Utility Model Content

[0005] The purpose of this invention is to provide a device for preparing microbial outer membrane vesicles, so as to solve the problem that the yield of outer membrane vesicles fluctuates due to the influence of the external environment and the distribution of the culture medium during the preparation of microbial outer membrane vesicles in the above-mentioned background technology.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a microbial outer membrane vesicle preparation device, comprising a high-pressure homogenizer body and a centrifuge, wherein a culture element is provided at one end of the top of the high-pressure homogenizer body and a ventilation element is provided at the other end of the top of the high-pressure homogenizer body;

[0007] The culture unit includes a culture cylinder detachably mounted on the top of the high-pressure homogenizer body. A cap is threadedly connected to the top of the culture cylinder, and a motor is detachably mounted on the top of the cap. A stirring rod is detachably mounted on the output end of the motor via a coupling. The stirring rod passes through the cap and is inserted into the culture cylinder. A first water pump is fixedly mounted on the side wall of the culture cylinder. A first connecting sleeve is fixedly mounted on one side of the high-pressure homogenizer body. A first connecting pipe is detachably mounted between the first connecting sleeve and the first water pump. A pressure gauge is detachably mounted on the high-pressure homogenizer body. A pressure regulating valve is detachably mounted on the other side of the high-pressure homogenizer body. A second connecting pipe is detachably mounted on the high-pressure homogenizer body, and a collection device is detachably mounted at the end of the second connecting pipe.

[0008] Preferably, the collecting component includes a filter sleeve inserted into the end of a second connecting pipe, a second water pump detachably mounted on the bottom of the side wall of the filter sleeve, a third connecting pipe detachably mounted on the second water pump, and the end of the third connecting pipe inserted into the centrifuge.

[0009] Preferably, the culture tube has an observation window.

[0010] Preferably, the ventilation component includes a gas filter that is detachably mounted on the top of the high-pressure homogenizer body, and a gas flow meter is detachably mounted between the gas filter and the culture tube.

[0011] Preferably, the output end of the pressure regulating valve is threadedly connected to a second connecting sleeve, and the second connecting sleeve is threadedly connected to a second connecting pipe.

[0012] Preferably, a filter screen for removing impurities is inserted inside the filter sleeve.

[0013] Preferably, the centrifuge is hinged with a top cover, which has an installation hole for inserting a third connecting pipe. The centrifuge has a cavity inside, and a liquid storage dish is rotatably connected inside the cavity. A limit cap is inserted into the mouth of the liquid storage dish, and the limit cap has a through hole for inserting the third connecting pipe.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] 1. In this utility model, the culture medium is directly connected to the main body of the high-pressure homogenizer. The culture medium is automatically transferred from the culture cylinder to the high-pressure homogenizer through the first water pump and the first connecting pipe, eliminating the need for manual operation. This reduces the chance of contact between the external environment and the materials, effectively reducing the risk of contamination and solving the problem in the prior art where "the culture device and the high-pressure homogenizer are independent devices, and manual transfer is easily subject to external contamination." The culture medium is equipped with a motor-driven stirring rod, which can continuously and stably stir the microbial culture medium in the culture cylinder, ensuring that the microorganisms are evenly distributed in the culture medium and avoiding local high concentrations or uneven nutrition, which is conducive to the stable growth of microorganisms. At the same time, the venting component filters the gas entering the culture cylinder through a gas filter and precisely controls the gas flow rate through a gas flow meter, providing a suitable gas environment for microorganisms, further optimizing growth conditions, and thus reducing fluctuations in the production of outer membrane vesicles.

[0016] 2. In this utility model, the device integrates high-pressure homogenization, filtration, and centrifugation functions. After high-pressure homogenization, the material enters the filter sleeve through the second connecting pipe. After impurities are removed by the filter screen, it enters the centrifuge for separation through the second water pump and the third connecting pipe, forming an integrated process of "cultivation-homogenization-filtration-centrifugation". This eliminates the need for multiple material transfers and shortens the preparation cycle. Furthermore, the filtration stage can remove large particulate impurities in advance, reducing the load on the centrifuge and improving the purity of the final outer membrane vesicles. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of a microbial outer membrane vesicle preparation device proposed in this utility model;

[0018] Figure 2 This is a schematic diagram of the internal structure of a centrifuge for preparing microbial outer membrane vesicles according to the present invention.

[0019] In the diagram: 1. High-pressure homogenizer body; 11. Pressure gauge; 12. Pressure regulating valve; 13. Second connecting pipe; 14. First connecting sleeve; 15. First connecting pipe; 16. First water pump; 17. Second connecting sleeve; 2. Culture medium; 21. Culture cylinder; 22. Cap; 23. Motor; 24. Observation window; 25. Stirring rod; 3. Ventilation component; 31. Gas filter; 32. Gas flow meter; 4. Filter sleeve; 41. Filter screen; 5. Centrifuge; 51. Second water pump; 52. Third connecting pipe; 53. Mounting hole; 54. Top cover; 55. Cavity; 56. Storage dish; 57. Limiting cap; 58. Through hole. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Example; please refer to Figure 1 - Figure 2 The diagram shows a device for preparing microbial outer membrane vesicles, including a high-pressure homogenizer body 1 and a centrifuge 5. A culture element 2 is provided at the top left end of the high-pressure homogenizer body 1, and a ventilation element 3 is provided at the top right end of the high-pressure homogenizer body 1. The ventilation element 3 includes a gas filter 31 that is detachably installed on the top of the high-pressure homogenizer body 1, and a gas flow meter 32 that is detachably installed between the gas filter 31 and the culture tube 21. Gas is introduced into the culture tube 21 through the ventilation element 3. After being filtered by the gas filter 31, the gas flow rate is controlled by the gas flow meter 32 to enter the culture tube 21, providing a suitable gaseous environment for microbial growth.

[0022] The culture medium 2 includes a culture cylinder 21 detachably mounted on the top of the high-pressure homogenizer body 1. A cap 22 is threadedly connected to the top of the culture cylinder 21. A motor 23 is detachably mounted on the top of the cap 22. A stirring rod 25 is detachably mounted on the output end of the motor 23 via a coupling. The stirring rod 25 passes through the cap 22 and is inserted into the culture cylinder 21. An observation window 24 is provided on the culture cylinder 21, allowing real-time observation of the culture medium's state. A first water pump 16 is fixedly mounted on the side wall of the culture cylinder 21. A first connecting sleeve 14 is fixedly mounted on the left side of the high-pressure homogenizer body 1. A first connecting pipe 15 is detachably mounted between the first connecting sleeve 14 and the first water pump 16. A pressure gauge 11 is detachably installed on the main body 1 of the high-pressure homogenizer. A pressure regulating valve 12 is detachably installed on the right side of the main body 1 of the high-pressure homogenizer. The output end of the pressure regulating valve 12 is threadedly connected to a second connecting sleeve 17. A second connecting pipe 13 is threadedly connected to the inner end of the second connecting sleeve 17. The homogenization pressure is adjusted by the pressure regulating valve 12. The homogenized material enters the filter sleeve 4 through the second connecting pipe 13. A filter screen 41 for removing impurities is inserted inside the filter sleeve 4. The filter screen 41 is made of stainless steel filter membrane. It is selected according to the target particle size of the outer membrane vesicles to ensure effective removal of impurities such as cell debris. A second connecting pipe 13 is detachably installed on the main body 1 of the high-pressure homogenizer. A collection device is detachably installed at the end of the second connecting pipe 13.

[0023] The collection component includes a filter sleeve 4 inserted into the right end of the second connecting pipe 13. A second water pump 51 is detachably installed on the bottom right side of the filter sleeve 4. A third connecting pipe 52 is detachably installed on the second water pump 51. The end of the third connecting pipe 52 is inserted into the centrifuge 5. A top cover 54 is hinged to the centrifuge 5. The top cover 54 has an installation hole 53 for the third connecting pipe 52 to be inserted. A cavity 55 is opened inside the centrifuge 5. A liquid storage dish 56 is rotatably connected inside the cavity 55. A limit cap 57 is inserted into the mouth of the liquid storage dish 56. A through hole 58 for the third connecting pipe 52 to be inserted is opened on the limit cap 57.

[0024] Working principle:

[0025] Microbial culture medium is injected into culture cylinder 21, and cap 22 is placed on top. Motor 23 is started, driving stirring rod 25 to stir the culture medium, so that the microorganisms are evenly distributed. At the same time, gas is introduced into culture cylinder 21 through venting device 3. After being filtered by gas filter 31, the gas flow rate is controlled by gas flow meter 32 to enter culture cylinder 21, providing a suitable gas environment for microbial growth. The state of the culture medium can be observed in real time through observation window 24. After the culture is completed, the first water pump 16 is started, and the culture medium enters the high-pressure homogenizer body 1 through the first connecting pipe 15 and the first connecting sleeve 14. The homogenization pressure is adjusted by pressure regulating valve 12 and the pressure value is monitored by pressure gauge 11, so that the material undergoes shearing, impact and other actions under high pressure, realizing the release and homogenization of microbial outer membrane vesicles.

[0026] After homogenization, the material enters the filter sleeve 4 through the second connecting pipe 13 and is filtered by the filter screen 41 to remove impurities. The second water pump 51 is started, and the filtered material enters the storage dish 56 of the centrifuge 5 through the third connecting pipe 52. The top cover 54 of the centrifuge 5 is closed, and the third connecting pipe 52 is kept connected through the mounting hole 53 and the through hole 58. The centrifuge 5 is started, and the storage dish 56 rotates at high speed in the cavity 55. The centrifugal force is used to separate the outer membrane vesicles from other components, and the preparation and collection of microbial outer membrane vesicles are finally completed.

[0027] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus.

[0028] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for preparing microbial outer membrane vesicles, comprising a high-pressure homogenizer body (1) and a centrifuge (5), characterized in that: The high-pressure homogenizer body (1) has a culture element (2) at one end of its top and a ventilation element (3) at the other end of its top. The culture medium (2) includes a culture cylinder (21) detachably mounted on the top of the high-pressure homogenizer body (1). A cap (22) is threadedly connected to the top of the culture cylinder (21). A motor (23) is detachably mounted on the top of the cap (22). A stirring rod (25) is detachably mounted on the output end of the motor (23) via a coupling. The stirring rod (25) passes through the cap (22) and is inserted into the culture cylinder (21). A first water pump (16) is fixedly mounted on the side wall of the culture cylinder (21). The high-pressure homogenizer... A first connecting sleeve (14) is fixedly installed on one side of the homogenizer body (1). A first connecting pipe (15) is detachably installed between the first connecting sleeve (14) and the first water pump (16). A pressure gauge (11) is detachably installed on the high-pressure homogenizer body (1). A pressure regulating valve (12) is detachably installed on the other side of the high-pressure homogenizer body (1). A second connecting pipe (13) is detachably installed on the high-pressure homogenizer body (1). A collection component is detachably installed at the end of the second connecting pipe (13).

2. The microbial outer membrane vesicle preparation device according to claim 1, characterized in that: The collection device includes a filter sleeve (4) inserted into the end of a second connecting pipe (13), a second water pump (51) is detachably installed on the bottom of the side wall of the filter sleeve (4), a third connecting pipe (52) is detachably installed on the second water pump (51), and the end of the third connecting pipe (52) is inserted into a centrifuge (5).

3. The microbial outer membrane vesicle preparation device according to claim 1, characterized in that: An observation window (24) is provided on the culture tube (21).

4. The microbial outer membrane vesicle preparation device according to claim 1, characterized in that: The ventilation component (3) includes a gas filter (31) that is detachably installed on the top of the high-pressure homogenizer body (1), and a gas flow meter (32) is detachably installed between the gas filter (31) and the culture tube (21).

5. The microbial outer membrane vesicle preparation device according to claim 1, characterized in that: The output end of the pressure regulating valve (12) is threadedly connected to a second connecting sleeve (17), and the second connecting sleeve (17) is threadedly connected to a second connecting pipe (13).

6. The microbial outer membrane vesicle preparation device according to claim 2, characterized in that: The filter sleeve (4) is fitted with a filter screen (41) for removing impurities.

7. The apparatus for preparing microbial outer membrane vesicles according to claim 2, characterized in that: The centrifuge (5) is hinged with a top cover (54), and the top cover (54) has an installation hole (53) for inserting a third connecting pipe (52). The centrifuge (5) has a cavity (55) inside, and a liquid storage dish (56) is rotatably connected inside the cavity (55). A limit cap (57) is inserted into the mouth of the liquid storage dish (56), and the limit cap (57) has a through hole (58) for inserting the third connecting pipe (52).

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