A pu tube ventilation device in a haematococcus pluvialis algae serum bottle culture

Abstract

The application relates to the technical field of Haematococcus pluvialis culture, and discloses a PU pipe ventilation device in Haematococcus pluvialis algal seed serum bottle culture, which comprises an upper PU straight pipe part, a lower PU straight pipe part and a spiral PU pipe part, and the top end of the spiral PU pipe part is fixedly connected with the bottom end of the lower PU straight pipe part. The application has the following advantages and effects: the circular-hole type air outlet is used to release tiny air bubbles, the air bubbles drive the Haematococcus pluvialis algal seed and the culture solution to flow in the rising process, play a stirring role, prevent the algal seed from sinking to the bottom, make the algal seed uniformly distributed, the triangular-prism-hole type air outlet generates spiral airflow, further mixes the possible sinking algal seed, enhances the flowability of the algal seed and the culture solution, ensures that the algal seed can fully contact nutrient substances and light, creates favorable conditions for high-density culture, effectively solves the problem that the algal seed is prone to precipitation in the Haematococcus pluvialis algal seed serum culture bottle, and the gas purification assembly is convenient to replace, can be recycled, and the cost can be saved.

Description

Technical Field

[0001] This application relates to the field of Haematococcus pluvialis culture technology, and in particular to a PU tube aeration device for Haematococcus pluvialis serum culture. Background Technology

[0002] In the cultivation of Haematococcus pluvialis, serum bottle culture is a common method in laboratories and for small-scale cultivation, which places high demands on the performance of the aeration device. Aeration not only needs to provide the algae with the gases (such as air or carbon dioxide) required for photosynthesis and respiration, but also needs to prevent the algae from settling to the bottom through airflow agitation, ensuring that they are evenly exposed to nutrients and light, thus laying the foundation for high-density cultivation.

[0003] However, existing PU tube aeration devices for Haematococcus pluvialis serum culture have at least the following shortcomings: First, some devices have straight tube aeration components with a single vent design, which can only generate unidirectional airflow. This leads to algal seeds easily depositing at the bottom of the bottle, poor fluidity of the culture medium, uneven distribution of algal seeds, and seriously affects photosynthetic efficiency and biomass accumulation. Second, they lack subsequent gas purification functions, which cannot effectively remove dust, bacteria and other impurities from the air, increasing the risk of algal seed contamination and restricting the algal seed culture effect.

[0004] Therefore, we propose a PU tube aeration device for Haematococcus pluvialis serum bottle culture to solve the above problems. Utility Model Content

[0005] The purpose of this application is to provide a PU tube aeration device for Haematococcus pluvialis serum bottle culture, which can effectively prevent algae from settling to the bottom, ensure uniform distribution of algae, enhance the fluidity of algae and culture medium, ensure that algae can fully contact nutrients and light, create favorable conditions for high-density culture, and facilitate the replacement of gas purification components, thereby achieving the effects of recycling and cost saving.

[0006] The above-mentioned technical objective of this application is achieved through the following technical solution: a PU tube aeration device for Haematococcus pluvialis serum bottle culture, comprising an upper PU straight tube, a lower PU straight tube, and a spiral PU tube. The top end of the spiral PU tube is fixedly connected to the bottom end of the lower PU straight tube, and the spiral PU tube and the lower PU straight tube are integrally formed. The bottom outer wall of the spiral PU tube has multiple evenly distributed circular air outlets. The outer wall of the spiral PU tube has triangular prism-shaped air outlets located above the circular air outlets. The number of triangular prism-shaped air outlets is set to multiple and evenly distributed. The top end of the lower PU straight tube is fixedly connected to a filter cylinder. The top of the filter cylinder is open. A support plate is fixedly installed inside the filter cylinder. The top of the support plate has multiple evenly distributed air vents. A gas purification component is provided on the top of the support plate. A cylinder cover is fixedly fitted onto the upper PU straight tube and is detachably installed and fixed on the top of the filter cylinder.

[0007] A further provision of this application is that the gas purification assembly includes a mixed cellulose ester filter membrane, a polyester fiber filter screen, and filter cotton, wherein the mixed cellulose ester filter membrane is laid on top of the support plate, the polyester fiber filter screen is laid on top of the mixed cellulose ester filter membrane, and the filter cotton is laid on top of the polyester fiber filter screen.

[0008] A further configuration of this application is as follows: the cover includes a cover plate and an external threaded locking ring. The cover plate is fixedly sleeved on the upper PU straight tube. The bottom of the cover plate abuts against the top of the filter cylinder. The external threaded locking ring is fixedly installed at the bottom of the cover plate. The bottom end of the upper PU straight tube extends into the external threaded locking ring. An internal thread is provided on the inner wall of the top of the filter cylinder. The external threaded locking ring is installed in the filter cylinder through the internal thread.

[0009] A further provision of this application is that the bottom of the external threaded locking ring abuts against the filter cotton.

[0010] A further provision of this application is that a frustum-shaped bottle stopper is fixedly sleeved on the lower PU straight tube section, and the top of the frustum-shaped bottle stopper is fixedly connected to the bottom of the filter cartridge.

[0011] A further feature of this application is that the frustum-shaped stopper is made of silicone material.

[0012] A further feature of this application is that a gas flow regulating valve located above the cylinder cover is fixedly installed on the upper PU straight pipe section.

[0013] A further feature of this application is that a pipe connector is fixedly connected to the top end of the upper PU straight tube.

[0014] A further feature of this application is that the bottom end of the spiral PU tube is a closed structure, and a sinker steel wire is embedded and fixed at the bottom end of the spiral PU tube.

[0015] This application includes at least one of the following beneficial technical effects:

[0016] 1. This application utilizes round-hole vents to release microbubbles. As these bubbles rise, they cause the Haematococcus pluvialis seed and culture medium to flow, thus agitating the algae and preventing them from settling to the bottom. This ensures the algae are evenly distributed. The triangular-hole vents generate a swirling airflow, further mixing any algae that may have settled to the bottom. This enhances the fluidity of the algae and culture medium, further improving the even distribution of the Haematococcus pluvialis seed and ensuring that the algae can fully contact nutrients and light. This creates favorable conditions for high-density cultivation and effectively solves the problem of easy sedimentation of Haematococcus pluvialis seed in serum culture flasks.

[0017] 2. This application utilizes a gas purification component composed of a mixed cellulose ester filter membrane, a polyester fiber filter screen, and filter cotton. This component can effectively filter dust impurities, bacteria, fungi, and other microorganisms from the air or carbon dioxide gas, ensuring the cleanliness of the gas introduced into the Haematococcus pluvialis serum culture bottle, reducing the risk of contamination by other microorganisms, ensuring the sterility of the algae culture environment, and improving the success rate of algae culture.

[0018] 3. This application utilizes a cylinder cover consisting of a cover plate and an external threaded locking ring to facilitate the replacement of gas purification components. Furthermore, the bottom of the external threaded locking ring is designed to abut against the filter cotton, which can press and fix the mixed cellulose ester filter membrane, polyester fiber filter screen, and filter cotton on the top of the support plate, ensuring that the mixed cellulose ester filter membrane, polyester fiber filter screen, and filter cotton are firmly installed and preventing displacement that could affect the filtration effect.

[0019] 4. This application, by tightly sealing the frustum-shaped stopper at the mouth of the Haematococcus pluvialis serum culture bottle, can prevent unfiltered external air, dust, impurities, and bacteria from entering the bottle. By pulling out the frustum-shaped stopper, the spiral PU tube can be removed from the Haematococcus pluvialis serum culture bottle, facilitating the cleaning and disinfection of components such as the upper PU straight tube, lower PU straight tube, spiral PU tube, filter cartridge, cap, frustum-shaped stopper, and tube connectors, enabling subsequent reuse and achieving the effects of recycling and cost savings. Attached Figure Description

[0020] Figure 1 This is a front-view stereoscopic structural diagram of this embodiment.

[0021] Figure 2 yes Figure 1 A magnified structural diagram of part A in the middle.

[0022] Figure 3This is a front view sectional three-dimensional structural diagram of the filter cartridge.

[0023] Figure 4 This is a schematic diagram of the three-dimensional structure of the support plate.

[0024] Figure 5 This is a three-dimensional structural diagram of the cylinder cap.

[0025] Figure 6 This is a three-dimensional structural diagram of the Haematococcus pluvialis serum culture flask used in this embodiment.

[0026] Figure 7 This is a front view cross-sectional three-dimensional structural diagram of the Haematococcus pluvialis serum culture flask in use according to this embodiment.

[0027] In the diagram: 1. Upper PU straight tube section; 2. Lower PU straight tube section; 3. Spiral PU tube section; 4. Round hole-type air outlet; 5. Triangular prism-type air outlet; 6. Filter cylinder; 7. Support plate; 8. Ventilation hole; 9. Mixed cellulose ester filter membrane; 10. Polyester fiber filter screen; 11. Filter cotton; 12. Cylinder cap; 121. Cover plate; 122. External threaded locking ring; 13. Frustum-shaped bottle stopper; 14. Gas flow regulating valve; 15. Pipe connector; 16. Sinking steel wire; 17. Haematococcus pluvialis serum culture bottle. Detailed Implementation

[0028] The technical solution of this application will be clearly and completely described below with reference to specific embodiments. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0029] See Figures 1-7This application provides a PU tube aeration device for Haematococcus pluvialis serum culture, comprising an upper PU straight tube section 1, a lower PU straight tube section 2, and a spiral PU tube section 3. The top end of the spiral PU tube section 3 is fixedly connected to the bottom end of the lower PU straight tube section 2, and the spiral PU tube section 3 and the lower PU straight tube section 2 are integrally formed. Multiple evenly distributed circular air outlet holes 4 are provided on the bottom outer wall of the spiral PU tube section 3. Triangular prism-shaped air outlet holes 5 are provided on the outer wall of the spiral PU tube section 3 above the circular air outlet holes 4. The number of triangular prism-shaped air outlet holes 5 is set to multiple, and the multiple triangular prism-shaped air outlet holes 5 are evenly distributed. By designing multiple evenly distributed circular air outlet holes 4, microbubbles are formed in the culture medium during aeration. These bubbles, as they rise, drive the flow of Haematococcus pluvialis and the culture medium. The stirring mechanism ensures uniform distribution of Haematococcus pluvialis in the culture medium, effectively preventing sedimentation of the algae in the Haematococcus pluvialis serum culture bottle 17. Multiple evenly distributed triangular prism-shaped vents 5 generate a swirling airflow during ventilation, mixing the sedimented algae in the Haematococcus pluvialis serum culture bottle 17, further increasing the fluidity of the Haematococcus pluvialis and the culture medium, and further enhancing the uniform distribution of the algae. This allows the algae to fully contact nutrients and light, laying a good foundation for high-density cultivation and improving algal biomass and astaxanthin accumulation. A filter cylinder 6 is fixedly connected to the top of the lower PU straight tube 2. The top of the filter cylinder 6 is open, and a support plate 7 is fixedly installed inside the filter cylinder 6. The top of the support plate 7 has multiple evenly distributed vent holes 8.

[0030] In this embodiment, a gas purification component is provided on the top of the support plate 7. The gas purification component is designed to effectively remove dust, bacteria, and other impurities from the air or carbon dioxide gas. The gas purification component includes a mixed cellulose ester filter membrane 9, a polyester fiber filter screen 10, and filter cotton 11. The mixed cellulose ester filter membrane 9 is laid on the top of the support plate 7, the polyester fiber filter screen 10 is laid on top of the mixed cellulose ester filter membrane 9, and the filter cotton 11 is laid on top of the polyester fiber filter screen 10. The filter cotton 11 is used to filter out large particulate dust impurities from the air or carbon dioxide gas, the polyester fiber filter screen 10 is used to filter out fine particulate dust impurities from the air or carbon dioxide gas, and the mixed cellulose ester filter membrane 9 is used to trap and filter bacteria, fungi, and other microorganisms from the air or carbon dioxide gas. This ensures that the gas introduced into the Haematococcus pluvialis serum culture bottle 17 is clean, reduces the risk of bacterial contamination, ensures the sterility of the algae culture environment, and improves the success rate of algae culture.

[0031] In this embodiment, a cylinder cover 12 is fixedly sleeved on the upper PU straight tube 1. The cylinder cover 12 is used to seal the top opening of the filter cylinder 6. The cylinder cover 12 is detachably installed and fixed on the top of the filter cylinder 6. The cylinder cover 12 includes a cover plate 121 and an external threaded locking ring 122. The cover plate 121 is fixedly sleeved on the upper PU straight tube 1. The bottom of the cover plate 121 abuts against the top of the filter cylinder 6. The external threaded locking ring 122 is fixedly installed at the bottom of the cover plate 121. The bottom end of the upper PU straight tube 1 extends into the external threaded locking ring 122. An internal thread is provided on the top inner wall of the filter cylinder 6. The external threaded locking ring 122 is installed in the filter cylinder 6 through the internal thread. By using the external threaded locking ring 122 to connect and match with the internal thread in the filter cylinder 6, it is convenient to disassemble and install the cylinder cover 12, thereby facilitating the replacement of the mixed cellulose ester filter membrane 9, polyester fiber filter screen 10 and filter cotton 11. The operation is simple and convenient.

[0032] In this embodiment, the bottom of the external threaded locking ring 122 abuts against the filter cotton 11. By designing the bottom of the external threaded locking ring 122 to abut against the filter cotton 11, the mixed cellulose ester filter membrane 9, the polyester fiber filter screen 10, and the filter cotton 11 can be pressed and fixed above the support plate 7, ensuring that the mixed cellulose ester filter membrane 9, the polyester fiber filter screen 10, and the filter cotton 11 are firmly installed and avoiding displacement that would affect the filtration effect.

[0033] In this embodiment, a frustum-shaped bottle stopper 13 is fixedly sleeved on the lower PU straight tube 2. The top of the frustum-shaped bottle stopper 13 is fixedly connected to the bottom of the filter cylinder 6. The frustum-shaped bottle stopper 13 is made of silicone material. The frustum-shaped bottle stopper 13 made of silicone material has good sealing performance and can be tightly sealed at the mouth of the Haematococcus pluvialis serum culture bottle 17 to prevent external air, dust impurities, and bacteria from entering the bottle without filtration.

[0034] In this embodiment, a gas flow regulating valve 14 is fixedly installed on the upper PU straight pipe section 1 above the cylinder cover 12. The gas flow regulating valve 14 is designed to precisely control the amount of air or carbon dioxide gas introduced according to the needs of Haematococcus pluvialis at different growth stages, so as to meet the gas requirements of algae at different growth stages.

[0035] In this embodiment, a pipe connector 15 is fixedly connected to the top end of the upper PU straight pipe section 1. The design of the pipe connector 15 facilitates connection to a gas source (air or carbon dioxide gas).

[0036] In this embodiment, the bottom end of the spiral PU tube 3 is a closed structure, and a sinking steel wire 16 is installed and fixed at the bottom end of the spiral PU tube 3. The design of the sinking steel wire 16 can make the bottom end of the spiral PU tube 3 stably sink to the bottom of the Haematococcus pluvialis serum culture bottle 17, avoiding positional displacement due to buoyancy and other factors, and ensuring stable ventilation position.

[0037] In this embodiment, as Figure 6 and Figure 7 As shown, by pouring the Haematococcus pluvialis strain and culture medium into the Haematococcus pluvialis serum culture bottle 17 in the prescribed ratio, then inserting the spiral PU tube 3 from the top of the Haematococcus pluvialis serum culture bottle 17, and then sealing the frustum-shaped stopper 13 into the bottle mouth, the Haematococcus pluvialis strain can be cultured.

[0038] With the above structure, when the PU tube ventilation device for Haematococcus pluvialis serum culture provided in this application is used, during the cultivation of Haematococcus pluvialis, according to the needs, by fixing the tube connector 15 to the outlet port of the external air storage bottle or carbon dioxide storage bottle, opening the gas flow regulating valve 14 and controlling its opening degree, air or carbon dioxide gas can be passed sequentially through the upper PU straight tube 1, filter cylinder 6, lower PU straight tube 2 and spiral PU tube 3, and finally the gas is divided into two paths, one path is discharged from multiple round hole-type air outlets 4, and the other path is discharged from multiple triangular prism-type air outlets 5. Then, according to the cultivation needs, by supplementing air or carbon dioxide into the Haematococcus pluvialis serum culture bottle 17, the gas requirements of Haematococcus pluvialis photosynthesis and respiration can be met, promoting its metabolism and ensuring the normal growth and reproduction of Haematococcus pluvialis;

[0039] Air or carbon dioxide gas passes through multiple layers of filtration in the filter cartridge 6, including filter cotton 11, polyester fiber filter screen 10, and mixed cellulose ester filter membrane 9. Filter cotton 11 first filters out large dust impurities in the gas, polyester fiber filter screen 10 further filters out fine dust impurities, and mixed cellulose ester filter membrane 9 traps and filters bacteria, fungi, and other microorganisms, thereby keeping the gas entering the lower PU straight pipe section 2 clean. This allows clean gas to be discharged from multiple round hole-type air outlets 4 and multiple triangular prism-type air outlets 5, avoiding contamination of the algae cultivation environment.

[0040] The purified gas enters the spiral PU tube section 3 through the lower straight PU tube section 2, and then exits from the round hole type 4 and the triangular prism type vent type 5 on the spiral PU tube section 3. During this process, the round hole type vent type 4 at the bottom releases tiny bubbles. As the bubbles rise, they drive the Haematococcus pluvialis seed and culture medium to flow, which plays a stirring role, prevents the algae seed from sinking to the bottom, and makes the algae seed evenly distributed. The triangular prism type vent type 5 at the top generates a swirling airflow, which further mixes any algae seed that may have settled to the bottom, enhances the fluidity of the algae seed and culture medium, and ensures that the algae seed can fully contact nutrients and light, creating favorable conditions for high-density culture. This effectively solves the problem of easy precipitation of algae seed in Haematococcus pluvialis serum culture bottles.

[0041] After the later stage of cultivation, the spiral PU tube 3 can be pulled out of the Haematococcus pluvialis serum culture bottle 17 by removing the frustum-shaped stopper 13. The cap 12 can be disassembled by unscrewing the external threaded locking ring 122 out of the filter cylinder 6. Then, the filter cotton 11, polyester fiber filter screen 10 and mixed cellulose ester filter membrane 9 can be removed and replaced in sequence using tweezers or other tools. The upper PU straight tube 1, lower PU straight tube 2, spiral PU tube 3, filter cylinder 6, cap 12, frustum-shaped stopper 13, and tube connector 15 can be cleaned and disinfected for subsequent reuse, thereby achieving the effect of recycling and saving costs.

Claims

1. A PU tube aeration device for serum bottle culture of Haematococcus pluvialis, characterized in that, The device includes an upper PU straight tube section (1), a lower PU straight tube section (2), and a spiral PU tube section (3). The top end of the spiral PU tube section (3) is fixedly connected to the bottom end of the lower PU straight tube section (2), and the spiral PU tube section (3) and the lower PU straight tube section (2) are integrally formed. The bottom outer wall of the spiral PU tube section (3) is provided with multiple evenly distributed round hole-type air outlets (4). The outer wall of the spiral PU tube section (3) is provided with triangular prism-type air outlets (5) located above the round hole-type air outlets (4). The number of ) is set to multiple, and the multiple triangular prism-type air outlets (5) are evenly distributed. The top end of the lower PU straight tube (2) is fixedly connected to the filter cylinder (6). The top of the filter cylinder (6) is an open structure. The filter cylinder (6) is fixedly installed inside the filter cylinder (6). The top of the support plate (7) is provided with multiple evenly distributed air vents (8). The top of the support plate (7) is provided with a gas purification component. The upper PU straight tube (1) is fixedly fitted with a cylinder cover (12). The cylinder cover (12) is detachably installed and fixed on the top of the filter cylinder (6).

2. The PU tube aeration device for Haematococcus pluvialis serum culture according to claim 1, characterized in that: The gas purification assembly includes a mixed cellulose ester filter membrane (9), a polyester fiber filter screen (10), and a filter cotton (11). The mixed cellulose ester filter membrane (9) is laid on top of the support plate (7), the polyester fiber filter screen (10) is laid on top of the mixed cellulose ester filter membrane (9), and the filter cotton (11) is laid on top of the polyester fiber filter screen (10).

3. The PU tube aeration device for Haematococcus pluvialis serum culture according to claim 2, characterized in that: The cover (12) includes a cover plate (121) and an external threaded locking ring (122). The cover plate (121) is fixedly sleeved on the upper PU straight tube (1). The bottom of the cover plate (121) abuts against the top of the filter cylinder (6). The external threaded locking ring (122) is fixedly installed at the bottom of the cover plate (121). The bottom end of the upper PU straight tube (1) extends into the external threaded locking ring (122). An internal thread is provided on the inner wall of the top of the filter cylinder (6). The external threaded locking ring (122) is installed in the filter cylinder (6) through the internal thread.

4. The PU tube aeration device for Haematococcus pluvialis serum culture according to claim 3, characterized in that: The bottom of the external threaded locking ring (122) abuts against the filter cotton (11).

5. The PU tube aeration device for Haematococcus pluvialis serum culture according to claim 1, characterized in that: A frustum-shaped bottle stopper (13) is fixedly sleeved on the lower PU straight tube (2), and the top of the frustum-shaped bottle stopper (13) is fixedly connected to the bottom of the filter cylinder (6).

6. The PU tube aeration device for Haematococcus pluvialis serum culture according to claim 5, characterized in that: The frustum-shaped stopper (13) is made of silicone material.

7. The PU tube aeration device for Haematococcus pluvialis serum culture according to claim 1, characterized in that: A gas flow regulating valve (14) is fixedly installed on the upper PU straight pipe section (1) above the cylinder cover (12).

8. The PU tube aeration device for Haematococcus pluvialis serum culture according to claim 1, characterized in that: The top end of the upper PU straight tube (1) is fixedly connected to a pipe connector (15).

9. The PU tube aeration device for Haematococcus pluvialis serum culture according to claim 1, characterized in that: The bottom end of the spiral PU tube (3) is a closed structure, and a sinker steel wire (16) is installed and fixed at the bottom end of the spiral PU tube (3).

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