A polypeptide cation exchange layer enzyme cutting device

Abstract

The utility model provides a kind of polypeptide cation exchange layer enzyme cutting device, comprising: motor and inner flow inner rotary blade, the motor front side is equipped with a group of power transmission for the transmission rod, the coupling front side is equipped with a group of transmission case for carrying out right angle power transmission, the transmission case lower end outside is equipped with a group of sealing cover for the tank body is sealed cover, compared with prior art, the utility model has the beneficial effects as follows: polypeptide raw materials are added into the tank body inner cavity by the packing port of sealing cover, ensure that two groups of sealing rubber ring are completely embedded, then four groups of buckles are used to fix sealing cover and tank body, start outer pressure pump to adjust the pressure of inner cavity to set value, electric heating wire is uniformly heated with spiral structure temperature insulation inner shell, vacuum layer maintains temperature stability, motor drives transmission rod by coupling and transmission case, drives inner enzyme cutting blade and inner flow inner rotary blade to rotate, and the cation exchange layer enzyme cutting of polypeptide material is realized.

Description

Technical Field

[0001] This invention belongs to the field of polypeptide enzymatic digestion technology and relates to a polypeptide cation exchange layer enzymatic digestion device. Background Technology

[0002] When using cation exchange layers for peptides in enzymatic digestion, the instability of internal pressure is a significant drawback. This instability leads to fluctuations in the mobile phase flow rate during chromatography, thereby affecting the contact efficiency between the enzyme and substrate and the uniformity of the digestion reaction. The causes of this instability are usually related to the physical properties of the chromatography column, such as the compressibility of the column bed, temperature variations within the column, viscosity variations of the mobile phase, and uneven pressure distribution within the column. Conventional methods to address these drawbacks include optimizing the physical structure of the chromatography column, such as using more uniform packing materials, improving column bed stability, and controlling operating conditions, such as isothermal control and using a viscosity-stable mobile phase. However, these methods have limitations. For example, improving the column bed structure may require expensive materials and complex processes, while isothermal control and a viscosity-stable mobile phase increase the complexity and cost of experiments. Therefore, there is an urgent need for a peptide cation exchange layer enzymatic digestion device to solve these problems. Utility Model Content

[0003] To address the shortcomings of existing technologies, the purpose of this invention is to provide a polypeptide cation exchange layer enzymatic digestion device to solve the problems mentioned in the background section.

[0004] This utility model is achieved through the following technical solution: a polypeptide cation exchange layer enzyme digestion device, comprising: a motor and an inner guide inner swirl vane, a set of couplings for transmitting power to the transmission rod is provided on the front side of the motor, a set of transmission boxes for transmitting right-angle power is provided on the front side of the coupling, and a set of sealing covers for sealing the tank is provided on the outer side of the lower end of the transmission box.

[0005] The lower end of the sealing cap is provided with a set of canisters for preventing polypeptide substances. The canisters include an insulated outer shell and electric heating wires. Inside the insulated outer shell is a vacuum layer for maintaining pressure balance. Inside the vacuum layer is an insulated inner shell for isolating the temperature of the inner and outer sides of the canisters. The insulated inner shell is the same as the insulated outer shell and is made of ceramic fiber material. Inside the insulated inner shell are several sets of electric heating wires for regulating the temperature inside the canisters.

[0006] In a preferred embodiment, the heating wires are evenly arranged and arranged in a spiral structure inside the heat-insulating inner shell. The inner side of the heat-insulating inner shell is provided with a set of cavities for enzymatic digestion of the cation exchange layer in the polypeptide material.

[0007] In a preferred embodiment, a set of sealing rings is provided at the upper end of the tank where it contacts the sealing cap to maintain a stable internal seal. Similarly, a set of sealing rings is provided at the contact position between the sealing cap and the tank to maintain a stable contact between the sealing cap and the tank. The two sets of sealing rings are sealed and fitted together.

[0008] In a preferred embodiment, the upper rear side of the sealing cap is provided with a set of external pressure pumps for adjusting the internal pressure of the inner cavity. Four sets of buckles are evenly distributed on the outer side of the connection between the sealing cap and the tank body to maintain its sealing limit. In actual use, the polypeptide raw material is first added to the inner cavity of the tank through the filling port of the sealing cap. After ensuring that the two sets of sealing rings are fully fitted, the sealing cap and the tank body are fixed with the four sets of buckles. The external pressure pump is started to adjust the internal cavity pressure to the set value. The electric heating wire heats the heat-insulating inner shell evenly in a spiral structure. The vacuum layer maintains temperature stability. The motor drives the transmission rod through the coupling and transmission box to drive the inner enzyme digestion blades and the inner guide inner swirl blades to rotate, thereby realizing the cation exchange layer enzyme digestion of the polypeptide material.

[0009] As a preferred embodiment, each set of buckles has a set of fastening bolts on its inner side for locking the sealing cap and the tank body to form a compression seal. Each set of fastening bolts passes through the inside of the buckle, and the lower end of the fastening bolts has a set of rubber pads for squeezing the upper end of the sealing cap.

[0010] In a preferred embodiment, the lower end of the motor is provided with a set of positioning seats for maintaining the motor's positioning, and the lower end of the transmission box is provided with a set of inner stabilizing columns for maintaining its rotational stability and reducing vibration potential energy. The inner stabilizing columns and the positioning seats are respectively positioned and fitted into the lower end of the transmission box and the lower end of the motor.

[0011] In a preferred embodiment, a set of transmission rods for transmitting motor power is provided at the lower end of the middle position of the transmission box, and an inner enzyme digestion blade for enzymatic digestion of the cation exchange layer in the polypeptide material is provided on the outer side of the middle position of the transmission rod, and the lower end of the inner enzyme digestion blade has a concave structure.

[0012] In a preferred embodiment, the inner side of the inner enzyme digestion blade is provided with a set of inner guiding spiral blades for circulating and guiding the polypeptide material. The inner guiding spiral blades are concave conical structures, and the inside of the inner guiding spiral blades is a concave spiral structure, which guides the polypeptide material from bottom to top in a spiral manner. In actual use, the motor fixed by the positioning seat is started, and the power is transmitted to the transmission rod of the embedded inner stabilizing column through the transmission box, which drives the inner enzyme digestion blades with concave structure to rotate. At the same time, the inner guiding spiral blades push the material from bottom to top in a spiral manner through the conical spiral structure, forming a continuous enzyme digestion environment. During the operation, the pressure in the inner cavity is maintained by an external pressure pump to stabilize the pressure. The electric heating wires are arranged in a spiral manner to precisely control the temperature of the heat-insulating inner shell. After the reaction is completed, the heating system is stopped first. After the temperature drops to a safe range, the fastening bolts are loosened in sequence to release the buckle lock, the bottom drain valve is opened to collect the enzyme digestion product, and finally the sealing cover assembly is disassembled to clean the equipment.

[0013] After adopting the above technical solution, the beneficial effects of this utility model are as follows: the polypeptide raw material is added into the inner cavity of the tank through the filling port of the sealing cap. After ensuring that the two sets of sealing rings are fully fitted, the sealing cap and the tank are fixed with four sets of buckles. The external pressure pump is started to adjust the pressure in the inner cavity to the set value. The electric heating wire heats the heat-insulating inner shell evenly in a spiral structure. The vacuum layer maintains temperature stability. The motor drives the transmission rod through the coupling and transmission box, which drives the inner enzyme digestion blade and the inner guide inner swirl blade to rotate, thereby realizing the enzymatic digestion of the cation exchange layer of the polypeptide material.

[0014] Power is transmitted from the transmission box to the drive rod of the embedded inner stabilizing column, which drives the inner enzyme digestion blades of the concave structure to rotate. At the same time, the inner guide blades push the material from the bottom to the top through the conical spiral structure, forming a continuous enzyme digestion environment. During operation, the pressure in the inner cavity is maintained by an external pressure pump. The electric heating wires are arranged in a spiral pattern to precisely control the temperature of the heat-insulating inner shell. After the reaction is completed, the heating system is stopped first. After the temperature drops to a safe range, the fastening bolts are loosened in sequence to release the buckle lock. The bottom drain valve is opened to collect the enzyme digestion product. Finally, the sealing cover assembly is disassembled to clean the equipment. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the left oblique front view of the enzymatic digestion device for polypeptide cation exchange layers according to this utility model;

[0017] Figure 2This is a top view of the inner stabilizing column and positioning seat in a polypeptide cation exchange layer enzymatic digestion device of the present invention.

[0018] Figure 3 This is a front view of the inner enzyme digestion blade in a polypeptide cation exchange layer enzyme digestion device of the present invention.

[0019] Figure 4 This is a front view schematic diagram of the internal structure of the tank in the polypeptide cation exchange layer enzymatic digestion device of this utility model;

[0020] In the diagram: 100-motor, 110-coupling, 120-sealing cover, 130-fastening bolt, 140-clasp, 150-tank body, 160-positioning seat, 170-inner stabilizing column, 180-shaft hole, 190-transmission rod, 200-inner enzyme digestion blade, 210-inner guide vane;

[0021] 15a - Thermal insulation outer shell, 15b - Vacuum layer, 15c - Thermal insulation inner shell, 15d - Electric heating wire. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Please see Figures 1-4 As the first embodiment of this utility model:

[0024] A polypeptide cation exchange layer enzymatic digestion device includes: a motor 100 and an inner guide vane 210. A set of couplings 110 for transmitting power to a transmission rod 190 is provided on the front side of the motor 100. A set of transmission boxes for transmitting power at right angles is provided on the front side of the couplings 110. A set of sealing caps 120 for sealing a tank 150 is provided on the outer side of the lower end of the transmission boxes.

[0025] The lower end of the sealing cap 120 is provided with a set of canisters 150 for preventing polypeptide substances. The canisters 150 include an insulated outer shell 15a and an electric heating wire 15d. Inside the insulated outer shell 15a, there is a set of vacuum layers 15b for maintaining pressure balance. Inside the vacuum layers 15b, there is a set of insulated inner shells 15c for isolating the temperature between the inside and outside of the canisters 150. The insulated inner shells 15c have the same specifications as the insulated outer shells 15a and are both made of ceramic fiber material. Inside the insulated inner shells 15c, there are several sets of electric heating wires 15d for regulating the internal temperature of the canisters 150.

[0026] The electric heating wires 15d are evenly arranged and arranged in a spiral structure inside the heat-insulating inner shell 15c. The inner side of the heat-insulating inner shell 15c is provided with a set of inner cavities for enzymatic digestion of the cation exchange layer in the polypeptide material.

[0027] A set of sealing rings is provided at the contact point between the upper end of the tank body 150 and the sealing cover 120 to maintain a stable internal seal. A set of sealing rings is also provided at the contact point between the sealing cover 120 and the tank body 150 to maintain a stable contact between the sealing cover 120 and the tank body 150. The two sets of sealing rings are sealed and fitted together.

[0028] A set of external pressure pumps for adjusting the internal pressure of the inner cavity is provided on the rear side of the upper end of the sealing cover 120. Four sets of buckles 140 are evenly distributed on the outer side of the connection between the sealing cover 120 and the tank body 150 to maintain its sealing limit. In actual use, the peptide raw material is first added to the inner cavity of the tank body 150 through the filling port of the sealing cover 120. After ensuring that the two sets of sealing rings are fully fitted, the sealing cover 120 and the tank body 150 are fixed with the four sets of buckles 140. The external pressure pump is started to adjust the pressure of the inner cavity to the set value. The electric heating wire 15d heats the heat-insulating inner shell 15c evenly in a spiral structure. The vacuum layer 15b maintains the temperature stability. The motor 100 drives the transmission rod 190 through the coupling 110 and the transmission box, which drives the inner enzyme digestion blade 200 and the inner guide inner swirl blade 210 to rotate, so as to realize the cation exchange layer enzyme digestion of the peptide material.

[0029] Please see Figures 1-4 As a second embodiment of the present invention: based on the description in the above embodiments, further, each set of buckles 140 is provided with a set of fastening bolts 130 for locking the sealing cap 120 and the tank body 150 by compression sealing. Each set of fastening bolts 130 penetrates the inside of buckles 140, and the lower end of the fastening bolts 130 is provided with a set of rubber pads for compressing the upper end of the sealing cap 120.

[0030] The lower end of the motor 100 is provided with a set of positioning seats 160 for maintaining the positioning of the motor 100, and the lower end of the transmission box is provided with a set of inner stabilizing columns 170 for maintaining its rotational stability and reducing vibration potential energy. The inner stabilizing columns 170 and the positioning seats 160 are respectively positioned and fitted into the lower end of the transmission box and the lower end of the motor 100.

[0031] A set of transmission rods 190 for transmitting power from motor 100 is provided at the lower middle position of the transmission box. An inner enzyme digestion blade 200 for enzymatic digestion of the cation exchange layer in polypeptide material is provided on the outer side of the middle position of the transmission rods 190. The lower end of the inner enzyme digestion blade 200 has a concave structure.

[0032] The inner side of the inner enzyme digestion blade 200 is provided with a set of inner guiding spiral blades 210 for circulating and guiding the polypeptide material. The inner guiding spiral blades 210 are concave conical structures, and the interior of the inner guiding spiral blades 210 is a concave spiral structure, which guides the polypeptide material spirally from bottom to top. In actual use, the motor 100 fixed by the starting positioning seat 160 is started, and the power is transmitted to the transmission rod 190 of the embedded inner stabilizing column 170 through the transmission box, which drives the inner enzyme digestion blades 200 with concave structure to rotate. The internal guide vane 210 uses a conical spiral structure to circulate and push the material from the bottom to the top, creating a continuous enzymatic digestion environment. During operation, the internal pressure is maintained by an external pressure pump. The electric heating wire 15d is arranged in a spiral pattern to precisely control the temperature of the heat-insulating inner shell 15c. After the reaction is completed, the heating system is stopped first. After the temperature drops to a safe range, the fastening bolts 130 are loosened to release the locking buckle 140. The bottom drain valve is opened to collect the enzymatic digestion product. Finally, the sealing cover 120 assembly is disassembled to clean the equipment.

[0033] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A polypeptide cation exchange layer enzymatic digestion device, comprising: The motor (100) and the inner guide vane (210) are characterized in that: a set of couplings (110) for transmitting power to the transmission rod (190) are provided on the front side of the motor (100), a set of transmission boxes for transmitting right-angle power are provided on the front side of the coupling (110), and a set of sealing covers (120) for sealing the tank (150) is provided on the outer side of the lower end of the transmission box. The lower end of the sealing cap (120) is provided with a set of cans (150) for preventing polypeptide substances. The cans (150) include a heat-insulating outer shell (15a) and an electric heating wire (15d). The heat-insulating outer shell (15a) is provided with a set of vacuum layers (15b) for maintaining pressure balance. The vacuum layer (15b) is provided with a set of heat-insulating inner shells (15c) for isolating the temperature inside and outside the cans (150). The heat-insulating inner shells (15c) are the same as the heat-insulating outer shells (15a) and are both made of ceramic fiber material. The heat-insulating inner shells (15c) are provided with several sets of electric heating wires (15d) for regulating the temperature inside the cans (150).

2. The polypeptide cation exchange layer enzymatic digestion device according to claim 1, characterized in that: The electric heating wires (15d) are evenly arranged and arranged in a spiral structure inside the heat-insulating inner shell (15c). The inner side of the heat-insulating inner shell (15c) is provided with a set of inner cavities for enzymatic digestion of the cation exchange layer in the polypeptide material.

3. The polypeptide cation exchange layer enzymatic digestion device according to claim 1, characterized in that: The upper end of the tank (150) is provided with a set of sealing rings at the contact point with the sealing cap (120) to maintain the internal sealing stability. The sealing cap (120) is also provided with a set of sealing rings at the contact point with the tank (150) to maintain the contact stability between the sealing cap (120) and the tank (150). The two sets of sealing rings are sealed and fitted together.

4. The polypeptide cation exchange layer enzymatic digestion device according to claim 3, characterized in that: The upper rear side of the sealing cover (120) is provided with a set of external pressure pumps for adjusting the internal pressure of the inner cavity. Four sets of buckles (140) are evenly distributed on the outer side of the connection between the sealing cover (120) and the tank body (150) to maintain its sealing limit.

5. The polypeptide cation exchange layer enzymatic digestion device according to claim 4, characterized in that: Each set of buckles (140) has a set of fastening bolts (130) inside for locking the sealing cap (120) and the tank body (150) with a squeeze seal. Each set of fastening bolts (130) passes through the inside of the buckle (140), and the lower end of the fastening bolts (130) has a set of rubber pads for squeezing the upper end of the sealing cap (120).

6. The polypeptide cation exchange layer enzymatic digestion device according to claim 1, characterized in that: The lower end of the motor (100) is provided with a set of positioning seats (160) for maintaining the positioning of the motor (100), and the lower end of the transmission box is provided with a set of inner stabilizing columns (170) for maintaining its rotational stability and reducing vibration potential energy. The inner stabilizing columns (170) and the positioning seats (160) are respectively positioned and fitted with the lower end of the transmission box and the lower end of the motor (100).

7. The polypeptide cation exchange layer enzymatic digestion device according to claim 6, characterized in that: The transmission box is provided with a set of transmission rods (190) at the lower end of the middle position for transmitting the power of the motor (100). The transmission rods (190) are provided with inner enzyme digestion blades (200) on the outer side of the middle position for enzymatic digestion of the cation exchange layer in the polypeptide material. The lower end of the inner enzyme digestion blades (200) is a concave structure.

8. The polypeptide cation exchange layer enzymatic digestion device according to claim 7, characterized in that: The inner side of the inner enzyme digestion blade (200) is provided with a set of inner guide inner spiral blades (210) for circulating and guiding polypeptide substances. The inner guide inner spiral blade (210) is a concave conical structure, and the interior of the inner guide inner spiral blade (210) is a concave spiral structure, which guides the polypeptide substances from bottom to top in a spiral.

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