A cooling device for polypeptide production

Abstract

The utility model relates to polypeptide production technical field discloses a polypeptide production cooling device, include: frame, fixed mounting on the reaction kettle of frame, cooling assembly and stirring subassembly that cool the reaction kettle. In the utility model, the water pump sends the refrigerated brine in the liquid storage tank to the water pipe no.

Description

Technical Field

[0001] This utility model relates to the field of peptide production technology, specifically to a peptide production cooling device. Background Technology

[0002] Cooling is a crucial step in peptide production (especially solid-phase synthesis SPPS and liquid-phase synthesis), primarily occurring during amino acid condensation (coupling) reactions.

[0003] In peptide production (especially on a medium to large scale), the reaction is typically carried out in a reactor equipped with a cooling jacket or built-in cooling coils. Chilled brine is circulated within the reactor's jacket or coils via a circulating pump, thus indirectly cooling the peptide synthesis materials (including mixtures of amino acids, activators, solvents, etc.) inside the reactor.

[0004] Patent application number CN202322358381.8 discloses a peptide production cooling device, relating to the field of cooling equipment technology. It includes a cooling component at the top of a cabinet, a connecting pipe at the bottom of the cooling component, a mounting plate at the bottom of the connecting pipe, and an air outlet at the bottom of the mounting plate. The cabinet is internally connected to a partition, and a fixed pipe is annularly hinged at the top of the partition at equal intervals. In this invention, a motor drives the rotating pipe to rotate an L-shaped rod and pulleys at the bottom of the placement plate. Simultaneously, the pulleys press against the bottom of the placement plate, causing it to shift via the hinged fixed pipes and support rods. This allows the peptides at the bottom of the placement box at the top of the placement plate to be shaken to the surface, facilitating the application of cold air to the surface of the peptides. Continuous operation of the motor repeatedly shakes the peptides out of the placement box, facilitating the cooling and freeze-drying of the peptides on the inner surface and bottom of the placement box, thus improving the efficiency of peptide cooling and freeze-drying.

[0005] However, this patent requires the peptide to be placed in a placement box and then placed inside a placement tank, which is not suitable for cooling during mass production of peptides.

[0006] Therefore, it is necessary to provide a new technical solution to overcome the above-mentioned defects. Utility Model Content

[0007] The purpose of this invention is to provide a peptide production cooling device that can effectively solve the above-mentioned technical problems.

[0008] To achieve the purpose of this utility model, the following technical solution is adopted: including: a frame; a reaction vessel fixedly installed on the frame; a cooling assembly and a stirring assembly for cooling the reaction vessel; and a cavity formed between the frame and the reaction vessel;

[0009] The cooling assembly includes: a coil fixedly installed on the cavity; a water pipe communicating with the coil; a water pump connected to the water pipe via a flange; and a liquid storage tank fixedly installed on the frame.

[0010] The water pump inlet is connected to a second water pipe; the second water pipe is connected to a storage tank.

[0011] Furthermore, a second motor is fixedly installed on the storage tank; a rotating shaft is fixedly installed on the second motor; and a second stirring blade is fixedly installed on the rotating shaft.

[0012] Furthermore, a turntable is fixedly installed at the end of the rotating shaft; a connecting rod is eccentrically installed on the turntable; a second filter screen is rotatably installed on the connecting rod, and a connecting rod is fixedly installed on the second filter screen; a first filter screen is fixedly installed on the connecting rod.

[0013] Furthermore, a fixing frame is fixedly installed on the liquid storage tank; a drain port is provided on the fixing frame; a limiting block is fixedly installed on the fixing frame; and the first filter screen and the second filter screen are slidably connected to the limiting block.

[0014] Furthermore, the stirring assembly includes: a motor fixedly mounted on the frame; a stirring shaft rotatably mounted on the reactor; and stirring blades fixedly mounted on the stirring shaft.

[0015] Furthermore, the motor drives the stirring shaft to rotate through a transmission structure.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a peptide production cooling device. A water pump draws frozen brine from the storage tank into the first water pipe through the second water pipe, and then into the coil. When the frozen brine flows in the coil, it transfers temperature to the reaction vessel, thereby cooling the peptides in the reaction vessel. The other end of the coil away from the stirring assembly is connected to the storage tank, so that the frozen brine can return to the storage tank. This completes the cooling of peptides without changing the container, resulting in higher cooling efficiency. When the water pump starts working, the second motor is started simultaneously, which drives the second stirring blade to rotate through the rotating shaft, thereby stirring the frozen brine, making the cooling temperature of the frozen brine more uniform and the cooling effect on the peptides better. Attached Figure Description

[0017] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0018] Figure 1 This is a schematic diagram of the structure of a polypeptide production cooling device according to the present invention;

[0019] Figure 2This is a cross-sectional structural diagram of a polypeptide production cooling device according to the present invention;

[0020] Figure 3 This is a schematic diagram of the fixed frame structure of a polypeptide production cooling device according to the present invention;

[0021] Figure 4 This utility model relates to a cooling device for polypeptide production. Figure 3 Enlarged structural diagram at point A in the middle.

[0022] In the diagram: 1. Frame; 2. Reactor; 3. Stirring assembly; 31. Motor 1; 32. Transmission structure; 33. Stirring shaft; 34. Stirring blade 1; 4. Cavity; 5. Cooling assembly; 51. Coil; 52. Water pipe 1; 53. Water pump; 54. Water pipe 2; 55. Storage tank; 551. Motor 2; 552. Rotating shaft; 553. Stirring blade 2; 554. Turntable; 555. Connecting rod; 556. Filter screen 2; 557. Connecting rod; 558. Filter screen 1; 559. Limiting block; 5510. Fixing frame; 5511. Drain port. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this utility model, but not all embodiments.

[0024] In the description of this utility model, it should be understood that the terms "center," "lateral," "longitudinal," "front," "rear," "left," "right," "upper," "lower," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this utility model. When a mechanism is referred to as being "fixed to" another mechanism, it can be directly on the other mechanism or there may be an intervening mechanism. When a mechanism is considered to be "connected" to another mechanism, it can be directly connected to the other mechanism or there may be an intervening mechanism at the same time. When a mechanism is considered to be "set on" another mechanism, it can be directly set on the other mechanism or there may be an intervening mechanism at the same time. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only.

[0025] like Figures 1 to 4As shown, this utility model discloses a peptide production cooling device, comprising: a frame 1, which is a metal support for supporting and protecting a reaction vessel 2; a reaction vessel 2 fixedly installed on the frame 1, the reaction vessel 2 being the core carrier of the peptide; a cooling component 5 for cooling the reaction vessel 2 and a stirring component 3. When the cooling component 5 cools the peptide in the reaction vessel 2, the stirring component 3 simultaneously starts to stir the peptide, thereby ensuring the uniformity of peptide cooling and avoiding uneven peptide cooling that would affect the finished product.

[0026] A cavity 4 is formed between the frame 1 and the reactor 2. The cooling assembly 5 includes: a coil 51 fixedly installed on the cavity 4, the coil 51 being spirally arranged to increase the contact range with the reactor 2 and improve the cooling effect; a water pipe 52 connected to the coil 51, the end of the coil 51 near the stirring assembly 3 being fixedly connected to the water pipe 52, the water pipe 52 passing through the frame 1; a water pump 53 connected to the flange of the water pipe 52, the water pipe 52 being connected to the outlet of the water pump 53, the water pump 53 being fixedly installed on the storage tank 55, the water pump 53 preferably being a circulating pump; a storage tank 55 fixedly installed on the frame 1, the storage tank 55 storing frozen brine for cooling the peptides, and a cooling plate fixedly installed on the side wall of the storage tank 55 to ensure the temperature of the frozen brine; a water pipe 54 connected to the inlet of the water pump 53; the water pipe 54 being connected to the storage tank 55.

[0027] Pump 53 draws the frozen brine from storage tank 55 into water pipe 52 via water pipe 2 54, and then into coil 51. As the frozen brine flows in coil 51, it transfers heat to reactor 2, thereby cooling the peptides in reactor 2. The other end of coil 51, away from stirring assembly 3, is connected to storage tank 55, allowing the frozen brine to return to storage tank 55. At this time, the frozen brine is cooled by the cooling plate in storage tank 55, ensuring the cooling effect of the frozen brine.

[0028] It should be noted that the temperature of the frozen brine near the cooling plate is low, and there is a temperature difference between the frozen brine and the frozen brine farther away from the cooling plate. This will lead to uneven temperature distribution of the frozen brine, affecting the cooling effect on the peptides. The storage tank 55 described in this application is fixedly installed with a second motor 551, which is a drive motor. A rotating shaft 552 is fixedly installed on the second motor 551. The rotating shaft 552 can be made of duplex stainless steel, which is low in cost and performs well in frozen brine. A stirring blade 553 is fixedly installed on the rotating shaft 552. When the water pump 53 starts working, the second motor 551 is started synchronously, driving the stirring blade 553 to rotate through the rotating shaft 552, thereby stirring the frozen brine, making the cooling temperature of the frozen brine more uniform, and improving the cooling effect on the peptides.

[0029] It should be noted that scale easily forms during the circulation of chilled brine. If left untreated, this scale will clog coil 51 as it circulates, affecting the cooling of the peptides. In this application, a turntable 554 is fixedly mounted at the end of the rotating shaft 552. A connecting rod 555 is eccentrically mounted on the turntable 554, and the connecting rod 555 is rotatably connected to the turntable 554. A second filter screen 556 is rotatably mounted on the connecting rod 555, and a connecting rod 557 is fixedly mounted on the second filter screen 556. A first filter screen 558 is fixedly mounted on the connecting rod 557. The chilled brine is thus filtered twice through both the first filter screen 558 and the second filter screen 556. The system is designed to effectively filter scale from the frozen brine. Both filter screens 558 and 556 are made of stainless steel, offering excellent corrosion resistance. A fixed frame 5510 is fixedly installed on the storage tank 55, and both filter screens 558 and 556 are installed within this frame. A drain port 5511 is provided on the fixed frame 5510. After being filtered by filter screens 558 and 556, the frozen brine enters the storage tank 55 through the drain port 5511. A limiting block 559 is fixedly installed on the fixed frame 5510, and the filter screens 558 and 556 are slidably connected to the limiting block 559.

[0030] When the rotating shaft 552 rotates, it drives the turntable 554 to rotate and simultaneously drives the filter screen 556 to slide back and forth along the limiting block 559 via the connecting rod 555 (the limiting block 559 limits the sliding of the filter screen 556). When the filter screen 556 moves back and forth, it shakes off the salt scale clogging the filter screen 556 through the impact with the chilled brine, thus preventing the filter screen 556 from clogging the mesh and affecting the filtration of the chilled brine. It should be noted that when the filter screen 556 moves, the connecting rod 557 drives the filter screen 558 to move back and forth synchronously along the limiting block 559, which can shake off the salt scale clogging the mesh on the filter screen 558, ensuring the filtration effect of the chilled brine.

[0031] In other words, when the rotating shaft 552 rotates, it drives the stirring blade 553 to rotate, thereby stirring the frozen brine and making the cooling temperature of the frozen brine more uniform and the cooling effect on peptides better. On the other hand, it drives the turntable 554 to rotate, which drives the filter screen 556 to move back and forth through the connecting rod 555. At the same time, the filter screen 558 moves back and forth along the limiting block 559 through the connecting rod 557. This shakes off the salt scale clogging the mesh on the filter screens 558 and 556 to prevent clogging of the mesh and thus affecting the filtration of the frozen brine, ensuring the effectiveness of the filter screens 558 and 556.

[0032] Finally, the stirring assembly 3 includes: a motor 31 fixedly mounted on the frame 1, preferably a drive motor; a stirring shaft 33 rotatably mounted on the reactor 2; and stirring blades 34 fixedly mounted on the stirring shaft 33. The motor 31 drives the stirring shaft 33 to rotate through a transmission structure 32. The transmission structure 32 can be any of the existing transmission structures 32, such as chain drive or belt drive. When using chain drive, the output shaft of the motor 31 and the stirring shaft 33 are fixedly connected to two sets of sprockets respectively, and then the two sets of sprockets are connected by a chain. If belt drive is used, the above motion mechanism can be referred to, and the sprockets can be replaced with pulleys, and the chain can be replaced with a belt.

[0033] When the cooling component 5 cools the peptide, the synchronous motor 31 drives the stirring shaft 33 to rotate through the transmission structure 32. Then, the stirring shaft 33 drives the stirring blade 34 to stir the peptide, so that the peptide is heated evenly and the temperature difference between peptides is avoided, which would affect the cooling of the peptide.

[0034] Working principle:

[0035] When cooling the peptides, water pump 53 is started to pump the chilled brine in storage tank 55 into water pipe 52 through water pipe 2 54, and then into coil 51. When the chilled brine flows in coil 51, it transfers the temperature to reactor 2, thereby cooling the peptides in reactor 2. Then, motor 2 551 is started to drive stirring blade 2 553 to rotate through rotating shaft 552, thereby stirring the chilled brine. At the same time, it drives turntable 554 to rotate. Through connecting rod 555, filter screen 2 556 slides back and forth along limiting block 559. When filter screen 2 556 moves, filter screen 1 558 moves back and forth along limiting block 559 synchronously through connecting rod 557, thereby shaking off the salt scale clogging the mesh on filter screen 1 558 and filter screen 2 556. Finally, motor 1 31 is started to drive stirring shaft 33 to rotate through transmission structure 32. Then, stirring blade 1 34 is driven by stirring shaft 33 to stir the peptides, so that the peptides are heated evenly.

[0036] All standard parts used in this utility model can be purchased from the market, and irregular parts can be customized according to the description and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art. In addition, the circuit connection adopts conventional connection methods in the prior art, which will not be described in detail here. The contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0037] It should be understood that those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims.

Claims

1. A peptide production cooling device, characterized in that, include: frame; A reaction vessel fixedly mounted on the frame; Cooling components and stirring components for cooling the reaction vessel; A cavity is formed between the frame and the reactor; The cooling assembly includes: a coil fixedly installed on the cavity; a water pipe communicating with the coil; and a water pump connected to the water pipe via a flange. A liquid storage tank fixedly installed on the frame; The water pump inlet is connected to a second water pipe; the second water pipe is connected to a storage tank.

2. The polypeptide production cooling apparatus as described in claim 1, characterized in that, A second motor is fixedly installed on the liquid storage tank; a rotating shaft is fixedly installed on the second motor; and a second stirring blade is fixedly installed on the rotating shaft.

3. The polypeptide production cooling apparatus as described in claim 2, characterized in that, A turntable is fixedly installed at the end of the rotating shaft; a connecting rod is eccentrically installed on the turntable; a second filter screen is rotatably installed on the connecting rod; a connecting rod is fixedly installed on the second filter screen; and a first filter screen is fixedly installed on the connecting rod.

4. The polypeptide production cooling apparatus as described in claim 3, characterized in that, A fixed frame is fixedly installed on the liquid storage tank; a drain port is opened on the fixed frame; a limit block is fixedly installed on the fixed frame; the first filter screen and the second filter screen are slidably connected to the limit block.

5. The polypeptide production cooling apparatus as described in claim 1, characterized in that, The stirring assembly includes: a motor fixedly mounted on the frame; a stirring shaft rotatably mounted on the reactor; and stirring blades fixedly mounted on the stirring shaft.

6. The polypeptide production cooling apparatus as described in claim 5, characterized in that, The motor drives the stirring shaft to rotate through a transmission structure.

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